CN106668110A - Artemisia argyi extract, eupatilin, jaceosidin and preparation method and application thereof - Google Patents
Artemisia argyi extract, eupatilin, jaceosidin and preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of medicine and relates to preparation of Artemisia argyi active extract and active monomer component and medicinal development and application in the aspects of gastric mucosa protection, anti-gastric ulcer and alcoholism prevention. The invention specifically provides an Artemisia argyi active extract and a preparation method thereof, jaceosidin, eupatilin and a preparation method thereof, and also relates to application of a composition thereof in the preparation of medicines, health-care products, functional food and food for gastric mucosa protection, anti-gastric ulcer and alcoholism prevention. The active extraction comprises the following steps: extracting Artemisia argyi with alcohol, concentrating the extract, recovering a solvent, drying to obtain an Artemisia argyi total extract, separating and purifying the total extract through macroporous resin chromatography to obtain an Artemisia argyi refined extract; or dispersing the above total extract in water, extracting with an isometric organic solvent, and recovering the solvent so as to obtain an Artemisia argyi refined extract.
Description
Technical field
The invention belongs to pharmaceutical technology field, comprising Folium Artemisiae Argyi activity extract, active monomer component
Preparation and protection gastric mucosa, anti-gastric-ulcer, relieve the effect of alcohol aspect drug development with application.
Background technology
Folium Artemisiae Argyi, from feverfew Chinese mugwort Artemisia argyi Levl.et Vant..Summer is spent not
Pluck when opening, remove impurity, dry.There are warming the meridian for stopping bleeding, dispersing cold for relieving pain, external removing dampness to relieve itching
Effect.It is mainly used in spitting blood, epistaxis, metrorrhagia, menorrhagia, vaginal bleeding during pregnancy hematochezia, few abdomen are cold
Bitterly, coldness and unbalance in meridians, the treatment of uterus cold infertile;External treatment skin pruritus.Vinegar Chinese mugwort charcoal warming the meridian for stopping bleeding,
For deficient cold type bleeding.Although Folium Artemisiae Argyi has great medical value, domestic and international even to this day
Still without any relevant its relevant report in terms of protecting gastric mucosa, anti-gastric-ulcer, greatly limit
Its application.Find in our early-stage Studies, this plant extract has preferably protection stomach
Biological activity in terms of mucosa, anti-gastric-ulcer, and the effect relieved the effect of alcohol.
Eupatilin (Eupatilin), the main active in Folium Artemisiae Argyi, pain relieving of radiating,
Warming the meridian for stopping bleeding, has treatment gastric ulcer and antispasmodic effect, moreover it is possible to regulating and controlling blood sugar.
Yellow powder, mp.231-233 DEG C.Ferric chloride-potassium ferricyanide reacting positive, points out
There is phenolic hydroxyl group;Hydrochloric acid-magnesium powder reacting positive, aluminum chloride reacting positive point out the chemical combination
Thing is flavone compound.1H-NMR (600MHz, DMSO-d6) in spectrum, δ 3.89
(3H, s ,-OCH3), 3.86 (3H, s ,-OCH3), 3.79 (3H, s ,-OCH3), 13.05 (1H,
S, 5-OH), 10.67 (1H, s, 7-OH), 7.52 (1H, br s, H-2 '), 7.08 (1H,
D, J=8.5Hz, H-5 '), 7.63 (1H, dd, J=8.5Hz, 1.0Hz, H-6 '), 6.91 (1H,
S, H-8), 6.62 (1H, s, H-3).
Jaceosidin (Jaceosidin), another main active in Folium Artemisiae Argyi,
Yellow powder, mp.229-230 DEG C.Ferric chloride-potassium ferricyanide reacting positive, points out
There is phenolic hydroxyl group;Hydrochloric acid-magnesium powder reacting positive, aluminum chloride reacting positive point out the chemical combination
Thing is flavone compound.1H-NMR (300MHz, DMSO-d6) in spectrum, δ 3.89
(3H, s ,-OCH3), 3.74 (3H, s ,-OCH3), 13.07 (1H, s, 5-OH),
7.56~7.54 (2H, m, H-5 ', 6 '), 6.94 (1H, d, J=9.0Hz, H-2 '), 6.58
(1H, s, H-8), 6.87 (1H, s, H-3).13C-NMR (75MHz, DMSO-d6):
δ 163.9 (C-2), 102.8 (C-3), 182.3 (C-4), 152.9 (C-5), 131.5 (C-6),
157.4 (C-7), 94.3 (C-8), 152.5 (C-9), 104.2 (C-10), 121.7 (C-1 '),
110.3 (C-2 '), 148.2 (C-3 '), 150.8 (C-4 '), 115.9 (C-5 '), 120.5 (C-6 '),
60.1(OCH3), 56.1 (OCH3)。
The content of the invention
It is an object of the invention to provide Folium Artemisiae Argyi activity extract and preparation method thereof, brown Centaurea cyanus
Element, eupatilin and preparation method thereof, further relate to combinations thereof thing and stick in preparation protection stomach
Film, answering in anti-gastric-ulcer, the medicine of aspect of relieving the effect of alcohol, health product, functional food, food
With.
The Folium Artemisiae Argyi extract that the present invention is provided, is to be prepared by the following method to obtain,
Take and be dried Folium Artemisiae Argyi (Artemisia argyi Levl.et Vant.) medical material, Jing alcohol extractions will
Extracting solution is concentrated, recycling design, is dried, is obtained Folium Artemisiae Argyi total extract.
The further Jing macroporous resin chromatography of total extract is isolated and purified, and obtains Folium Artemisiae Argyi essence extract;
Or above-mentioned total extract is dispersed in water, extracted with isopyknic organic solvent, recycling design,
Obtain Folium Artemisiae Argyi essence extract.
Wherein, alcohol-water solution of the alcohol of the extraction for 60-95%, preferably 75-95% alcohol water
Solution, extracting mode be dipping, percolation, be heated to reflux, laser heating backflow (Sharpe extraction
Method), supersound extraction.
Described Folium Artemisiae Argyi medical material includes feverfew Chinese mugwort Artemisia argyi L é vl.et Vant.'s
Dried leaves, stem or whole aerial partss.
Described alcohol is methanol or ethanol;Extraction time is 2-3 time;Solvent load and medical material matter
The ratio of amount is 1:4-1:12, the wherein ratio of each solvent load of seepage pressure effects and quality of medicinal material
For 1:4-1:20;The time of Soakage extraction is each 4-20 hours, be heated to reflux, or it is continuous plus
Hot reflux (Sharpe extraction method), or ultrasonic extracting method each extraction time be 0.5-4 hours;
The condensing mode is air-distillation or vacuum distillation, and drying mode is room temperature heat drying
Or drying under reduced pressure.
The macroporous adsorbent resin is isolated and purified, and is to use water successively, and volume ratio is 40-60%
The alcohol-water eluting of ethanol/water, 70-95%, is enriched with the ethanol/water eluent of 70-95%.Institute
State in step, it is air-distillation and vacuum distillation to reclaim condensing mode.
It is described extraction adopt organic solvent include chloroform (or dichloromethane), ethyl acetate,
One or two.
Two kinds of monomeric compound eupatilins, Jaceosidins that the present invention is provided, are to pass through
Following methods are prepared,
Above-mentioned Folium Artemisiae Argyi extract, Jing silica gel column chromatographies, using solvent gradient elution, prepare different pool
Blue flavin, Jaceosidin.
The method of gradient elution is:With volume ratio 50:1-10:1 dichloromethane (or chloroform)-
Methanol, preferred volume ratio 30:1-12.5:1 dichloromethane (or chloroform)-methanol system gradient
Eluting, obtains eupatilin, Jaceosidin monomeric compound;Or adopt volume ratio for
12:1-3:1 petroleum ether (or normal hexane)-acetone, preferred volume ratio 10:1-5:1 petroleum ether (or
Normal hexane)-acetone system gradient elution, obtain eupatilin, Jaceosidin singulation
Compound.
Above-mentioned Folium Artemisiae Argyi total extract, essence extract, eupatilin, Jaceosidin or it
With acceptable excipient, adjuvant in medicine, food, functional food and health product etc. etc.
The compositionss of composition, can be used to preparing the medicine of antitumor, gout and inhibiting hyperuricemia,
The application of health product, functional food and food.
Specific embodiment
The following examples will be further described to the present invention, but therefore not limited
The present invention.
Embodiment 1
Folium Artemisiae Argyi 4kg, with 12 times after shredding, heats at 10 times and 8 times of 80 DEG C of 60% alcohol and carries
Take three times, (3 hours for the first time, second 2 hours, third time 2 hours) filter, close
And filtrate, recycling design obtains total extract, yield about 15.5%;With eupatilin and palm fibre arrow
The total content meter of car centautin, content are about 1.45%.
Embodiment 2
Folium Artemisiae Argyi 4kg, with 20 times after crushing, at 8 times of 85 DEG C of 95% alcohol, warm macerating is extracted twice,
(4 hours for the first time, second 3 hours) filter, and merging filtrate, recycling design are obtained and always carried
Thing is taken, yield is about 8.3%;In terms of the total content of eupatilin and Jaceosidin, contain
Amount about 2.55%.
Embodiment 3
Folium Artemisiae Argyi 4kg, with 12 times after crushing, heats at 10 times and 8 times of 80 DEG C of 75% alcohol and carries
Take three times, (3 hours for the first time, second 2 hours, third time 0.5 hour) filter,
Merging filtrate, recycling design obtain extract, and yield is about 12.6%;With eupatilin and palm fibre
The total content meter of cyanidin, content are about 1.25%.
Embodiment 4
Folium Artemisiae Argyi 4kg, with 12 times after shredding, 10 times and 8 times of 80% alcohol dipping extracts three
Secondary (10 hours for the first time, second 6 hours, third time 2 hours), filter, merge filter
Liquid, recycling design obtain total extract, yield about 8.6%;With eupatilin and brown Centaurea cyanus
The total content meter of element, content are about 1.55%.
Embodiment 5
Folium Artemisiae Argyi 4kg, with 10 times after shredding, 8 times of 95% alcohol dipping extracts secondary (first
Secondary 10 hours, second 8 hours), filter, merging filtrate, recycling design obtain total extract,
Yield about 7.2%;In terms of the total content of eupatilin and Jaceosidin, content is about
2.30%.
Embodiment 6
Folium Artemisiae Argyi 4kg, with 12 times after shredding, 10 times, 8 times of 60% alcohol dipping is extracted three times
(10 hours for the first time, second 8 hours, third time 4 hours), filter, merging filtrate,
Recycling design obtains total extract, yield about 9.1%;With eupatilin and Jaceosidin
Total content meter, content are about 1.38%.
Embodiment 7
Folium Artemisiae Argyi 4kg, is extracted with 20 liter of 95% alcohol percolation after shredding, and is filtered, merging filtrate,
Recycling design obtains total extract, yield about 6.6%;With eupatilin and Jaceosidin
Total content meter, content are about 1.65%.
Embodiment 8
Folium Artemisiae Argyi 4kg, is extracted with 40 liter of 60% alcohol percolation after shredding, and is filtered, merging filtrate,
Recycling design obtains total extract, yield about 6.2%;With eupatilin and Jaceosidin
Total content meter, content are about 1.25%.
Embodiment 9
Folium Artemisiae Argyi 4kg, is extracted with 16 liter of 75% alcohol percolation after shredding, and is filtered, merging filtrate,
Recycling design obtains total extract, yield about 5.2%;With eupatilin and Jaceosidin
Total content meter, content are about 1.30%.
Embodiment 10
Folium Artemisiae Argyi 4kg, with 10 times after shredding, 8 times of 85% ethanol ultrasonic extraction secondary twice (
One time 1 hour, second 0.5 hour), filter, merging filtrate, recycling design are obtained and are always carried
Take thing, yield about 11.6%;In terms of the total content of eupatilin and Jaceosidin, content
About 1.98%.
Embodiment 11
Take Folium Artemisiae Argyi total extract to be dispersed in water, respectively with isopyknic petroleum ether, chloroform extraction,
Recycling design, obtains chloroform extract, and Folium Artemisiae Argyi essence extract is obtained after being dried, and yield is 3.9%;
In terms of the total content of eupatilin and Jaceosidin, its content in essence extract is about
6.9%.
Embodiment 12
Take Folium Artemisiae Argyi total extract to be dispersed in water, respectively with isopyknic petroleum ether, ethyl acetate
Extraction, recycling design obtain acetic acid ethyl ester extract, obtain Folium Artemisiae Argyi essence extract, receive after being dried
Rate is 4.5%;In terms of the total content of eupatilin and Jaceosidin, which is in essence extract
In content be about 5.6%.
Embodiment 13
Take Folium Artemisiae Argyi total extract to be dispersed in water, extracted with isopyknic petroleum ether, n-butyl alcohol respectively
Take, recycling design, obtain n-butyl alcohol extract, after being dried, obtain Folium Artemisiae Argyi essence extract, yield is
5.0%;In terms of the total content of eupatilin and Jaceosidin, its containing in essence extract
Amount about 4.3%.
Embodiment 14
Take Folium Artemisiae Argyi total extract, Jing HPD100 type macroporous adsorbent resin colors, successively with water, body
Product ratio is 60% ethanol/water, 95% alcohol-water eluting, and 95% ethanol/water eluent is dense
Contracting drying, obtains Folium Artemisiae Argyi essence extract, and yield is 3.7%;With eupatilin and palm fibre arrow car
The total content meter of centautin, its content in essence extract are about 6.7%.
Embodiment 15
Take Folium Artemisiae Argyi total extract, Jing AB-8 type macroporous adsorbent resin colors, successively with water, volume
Ratio is 40% ethanol/water, 70% alcohol-water eluting, 70% ethanol/water eluent concentration
It is dried, obtains Folium Artemisiae Argyi essence extract, yield is 4.2%;With eupatilin and brown Centaurea cyanus
The total content meter of element, its content in essence extract are about 4.0%.
Embodiment 16
Take Folium Artemisiae Argyi total extract, Jing D101 type macroporous adsorbent resin colors, successively with water, volume
Ratio is 55% ethanol/water, 80% alcohol-water eluting, 80% ethanol/water eluent concentration
It is dried, obtains Folium Artemisiae Argyi essence extract, yield is 3.9%;With eupatilin and brown Centaurea cyanus
The total content meter of element, its content in essence extract are about 5.8%.
Embodiment 17
Take Folium Artemisiae Argyi total extract, Jing silica gel column chromatographies, using methylene chloride-methanol mixed solvent
50:1、30:1、25:1、20:1、10:1 gradient elution, wherein from 25:1 eluting position, point
From two monomeric compound eupatilins and Jaceosidin is obtained, purity is respectively
99.0%th, 98.5%.
Embodiment 18
Take Folium Artemisiae Argyi total extract, Jing silica gel column chromatographies, using chloroform-methanol mixed solvent 40:1、
25:1、12.5:1 gradient elution, wherein from 25:1 eluting position, isolated two singulation
Compound eupatilin and Jaceosidin, purity are respectively 99.0%, 98.5%.
Embodiment 19
Take Folium Artemisiae Argyi essence extract, Jing silica gel column chromatographies, using chloroform-methanol mixed solvent 30:1、
25:1、20:1、12.5:1 gradient elution, wherein from 30:1 eluting position, isolated different pool
Blue flavin, purity are divided into 98.5%;From 25:1 eluting position, isolated Jaceosidin,
Purity is 99.0%.
Embodiment 20
Take Folium Artemisiae Argyi essence extract, Jing silica gel column chromatographies, using petroleum ether-acetone mixed solvent
12:1、10:1、8:1、5:1 gradient elution, wherein from 10:1 eluting position, it is isolated different
Eupatorin and Jaceosidin, purity are 98.5%.
Embodiment 21
Take Folium Artemisiae Argyi essence extract, Jing silica gel column chromatographies, using normal hexane-acetone mixed solvent
10:1、8:1、5:1、3:1 gradient elution, wherein from 8:1 eluting position, isolated different pool
Blue flavin and Jaceosidin, purity are 98.5%.
Embodiment 22
Folium Artemisiae Argyi extract and its active component eupatilin, Jaceosidin are to gastric mucosa injury
Protective effect (ethanol cause Ulcer Models).
(1) prepared by gastric mucosa injury model:After Rat Fast 24,30gL-1Pentobarbital
Sodium (4mgkg-1) intraperitoneal anesthesia, with l mL dehydrated alcohol gavages, after l h, median abdominal incision enters
Enter abdominal cavity, stomach is cut off at greater gastric curvature, and carries out every experimental index measure.
(2) it is administered and is grouped:SD rats 55 are taken, 5 groups are randomly divided into, per group 11
Only.I groups:Sham operated rats, as normal control;II groups:Damage model group, as damage
Control;III groups:Folium Artemisiae Argyi crude extract group, IV groups:Essence extract.Filled with dehydrated alcohol
Before stomach 30min, gavage Folium Artemisiae Argyi extract (200mgkg-1);V groups:Eupatilin group.
Before with dehydrated alcohol gavage 30min, tail vein injection eupatilin (20mgkg-1);
VI groups:Jaceosidin.Before with dehydrated alcohol gavage 30min, the different palm fibre of tail vein injection
Cyanidin (20mgkg-1)。
(3) Gastric mucosal injury index (Ulcer index, UI) judges:Calculate by Guth standards juice
Ulcer index, puts to death animal, takes out stomach and duodenum, after cutting off along big curved example, flattening,
Given and scored by ulcer or rotten to the corn size:It is mottled rotten to the corn for 1 point, it is rotten to the corn<1mm is
2 points, erosion is 3 points between 1-2mm, and rotten to the corn is 4 points for 2-4mm, rotten to the corn>
4mm is 5 points.
(4) histological observation:Full stomach is fixed on into formalin solution, paraffin section is made,
HE is dyeed, and light Microscopic observation, evaluation criteria are as follows:(1) downright bad material:Have no as 0, only
Have a little for (+) in ulcer bottom edge, it is (++) that thin layer covers whole ulcer bottom, thick-layer is covered
For (+++) (2) neutrophil infiltration:Have no as 0, accidental (+), hence it is evident that (+++), between rear
It is between the two (++).
As a result:
(1) UI is determined:Damage model group rat UI (25.33 ± 2.47) compares Normal group
Significantly raised (the P of UI (0)<0.01);Folium Artemisiae Argyi extract group, eupatilin group and Jaceosidin
Group is with damage model group than substantially reducing (P<0.01).
(2) gastric tissue observation:The visible a large amount of sphaceluss in damage model group rat ulcer bottom
Matter, in surrounding tissue, neutrophil infiltration is obvious;It is Folium Artemisiae Argyi extract group (III, IV), different
Neutral grain in eupatorin group and Jaceosidin group ulcer bottom necrosis material and surrounding tissue
Cellular infiltration substantially mitigates (P than damage model group<0.01).The results are shown in Table l.
Table l each group rat pipe film injury indexes
Change and histological observation (n=11, X ± S)
Note:Compare * * P with II group<0.01
Conclusion:Folium Artemisiae Argyi crude extract and essence extract, and its main component eupatilin,
Jaceosidin has significant gastric mucosal protective effect, can be used for resisting ethanol etc. it is various because
Damage of the element to gastric mucosa, and there are prevention and treatment to gastric ulcer.
Embodiment 23
Folium Artemisiae Argyi extract and its active component eupatilin, Jaceosidin are to gastric mucosa injury
Protective effect (water Stress model).
(1) prepared by gastric mucosa injury model:Before test, Rat Fast 12 hours, rat is tied
It is tied in special template, is put in 25 DEG C of water-baths, make the flat xiphoid-process of the water surface, puts to death after 18h
Animal, median abdominal incision enter abdominal cavity, cut off stomach at greater gastric curvature, and carry out every experiment and refer to
Mapping is fixed.
(2) it is administered and is grouped:SD rats 55 are taken, 5 groups are randomly divided into, per group 11
Only.I groups:Blank group, as normal control;II groups:Damage model group is right as damaging
According to;III groups:Folium Artemisiae Argyi crude extract group, IV groups:Essence extract.Prepare in water Stress model
First 7 days, daily gavage Folium Artemisiae Argyi extract (200mgkg-1);V groups:Eupatilin group.
Prepare first 7 days in water Stress model, daily tail vein injection eupatilin (20mgkg-1);
VI groups:Jaceosidin.Prepare first 7 days in water Stress model, daily tail vein injection is different
Jaceosidin (20mgkg-1)。
(3) Gastric mucosal injury index (Ulcer index, UI) judges:Calculate by Guth standards juice
Ulcer index, puts to death animal, takes out stomach and duodenum, after cutting off along big curved example, flattening,
Given and scored by ulcer or rotten to the corn size:It is mottled rotten to the corn for 1 point, it is rotten to the corn<1mm is
2 points, erosion is 3 points between 1-2mm, and rotten to the corn is 4 points for 2-4mm, rotten to the corn>
4mm is 5 points.
(4) histological observation:Full stomach is fixed on into formalin solution, paraffin section is made,
HE is dyeed, and light Microscopic observation, evaluation criteria are as follows:(1) downright bad material:Have no as 0, only
Have a little for (+) in ulcer bottom edge, it is (++) that thin layer covers whole ulcer bottom, thick-layer is covered
For (+++) (2) neutrophil infiltration:Have no as 0, accidental (+), hence it is evident that (+++), between rear
It is between the two (++).
As a result:
(1) UI is determined:Damage model group rat UI (19.68 ± 1.74) compares Normal group
Significantly raised (the P of UI (0)<0.01);Folium Artemisiae Argyi extract group (runic, essence are carried), eupatilin
Group and Jaceosidin group are with damage model group than substantially reducing (P<0.01).
(2) gastric tissue observation:The visible a large amount of sphaceluss in damage model group rat ulcer bottom
Matter, in surrounding tissue, neutrophil infiltration is obvious;Folium Artemisiae Argyi extract group (runic, essence are carried),
It is neutral in eupatilin group and Jaceosidin group ulcer bottom necrosis material and surrounding tissue
Granulocyte infiltration substantially mitigates (P than damage model group<0.01).The results are shown in Table 2.
2 each group rat pipe film injury index of table
Change and histological observation (n=11, X ± S)
Note:Compare * * P with II group<0.01
Conclusion:Folium Artemisiae Argyi crude extract and essence extract, and its main component eupatilin,
Jaceosidin to water stress caused by gastric mucosa injury there is significant protective effect, Ke Yiyong
In the damage to many factors such as anxieties to gastric mucosa, and there are prevention and treatment to gastric ulcer
Effect.
Claims (10)
1. Folium Artemisiae Argyi extract, it is characterised in that extract by the following method:
Take and be dried Folium Artemisiae Argyi medical material, extracting solution is concentrated, recycling design by Jing alcohol extractions, be dried, obtain Folium Artemisiae Argyi total extract.
2. Folium Artemisiae Argyi extract as claimed in claim 1, it is characterised in that Folium Artemisiae Argyi total extract Jing macroporous resin chromatography is isolated and purified and obtains Folium Artemisiae Argyi essence extract;Described macroporous adsorbent resin selects non-polar resin, low pole resin or middle polarity resin, described non-polar resin is selected from XAD-4, Diaion HP-20, D101, D102, D401, D1, D2, D3, D4, HPD-100 or X-5, described low pole resin is selected from D-201, HPD-300 or AB-8, described middle polarity resin is selected from XAD-6, XAD-7 or XAD-8, eluting uses water successively, volume ratio is 40-60% ethanol/waters, the alcohol-water eluting of 70-95%, the ethanol/water eluent of enrichment 70-95%, obtains Folium Artemisiae Argyi essence extract.
3. Folium Artemisiae Argyi extract as claimed in claim 1, it is characterized in that, Folium Artemisiae Argyi total extract is dispersed in water, Jing after petroleum ether or n-hexane extraction ungrease treatment, extracted with isopyknic organic solvent, recycling design, obtains Folium Artemisiae Argyi essence extract, extracts adopted organic solvent includes in chloroform/dichloromethane, ethyl acetate one or two.
4. the Folium Artemisiae Argyi extract according to claim 1-3 any one, it is characterised in that:The Folium Artemisiae Argyi medical material ends for feverfewArtemisia argyi Lévl.et
Vant. dried leaves, stem or whole aerial partss.
5. the Folium Artemisiae Argyi extract according to claim 1-4 any one, it is characterised in that:The determining alcohol be 60%-95% alcohol-water solution, preferred 75-95%, mode be dipping, percolation, be heated to reflux, or laser heating flow back(Sharpe extraction method), or supersound extraction.
6. the Folium Artemisiae Argyi extract according to claim 1-5 any one, it is characterised in that:Alcohol used by extracting is methanol or ethanol;Extraction time is 2-3 time;The ratio of solvent load and quality of medicinal material is 1:4-1:12, the wherein ratio of each solvent load of seepage pressure effects and quality of medicinal material is 1:4-1:20;The time of Soakage extraction be each 4-20 hours, be heated to reflux, or laser heating backflow(Sharpe extraction method), or ultrasonic extracting method each extraction time be 0.5-4 hours;Condensing mode is air-distillation or vacuum distillation, and drying mode is room temperature heat drying or drying under reduced pressure.
7. the preparation method of eupatilin, Jaceosidin, it is characterised in that by the Folium Artemisiae Argyi extract Jing silica gel column chromatographies described in claim 1-6 any one, using solvent gradient elution, obtain monomeric compound。
8. preparation method according to claim 7, it is characterised in that:The method of gradient elution is:With volume ratio 50:1-10:1 dichloromethane(Or chloroform)- methanol, preferred volume ratio 30:1-12.5:1 dichloromethane(Or chloroform)- methanol system gradient elution, obtains eupatilin, Jaceosidin monomeric compound;Or volume ratio is adopted for 12:1-3:1 petroleum ether(Or normal hexane)- acetone, preferred volume ratio 10:1-5:1 petroleum ether(Or normal hexane)- acetone system gradient elution, obtains eupatilin, Jaceosidin monomeric compound.
9. pharmaceutical composition, comprising acceptable excipient in the eupatilin described in the Folium Artemisiae Argyi extract described in claim 1-6 any one or claim 7-8 any one, Jaceosidin, with pharmacy, functional food, health product or food.
10. the pharmaceutical composition described in eupatilin or Jaceosidin or claim 9 described in Folium Artemisiae Argyi extract described in claim 1-6 any one or claim 7-8 any one is preparing gastric mucosal protection, anti-gastric-ulcer, the application in the medicine of the aspect such as relieve the effect of alcohol, health product, functional food and food.
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CN107550965A (en) * | 2017-08-28 | 2018-01-09 | 杨凌萃健生物工程技术有限公司 | A kind of preparation method of Folium Artemisiae Argyi extract |
CN110859864A (en) * | 2019-12-05 | 2020-03-06 | 江苏食品药品职业技术学院 | Preparation process of folium artemisiae argyi extract and application of folium artemisiae argyi extract in preparation of acute kidney injury medicine |
CN113827629A (en) * | 2021-06-07 | 2021-12-24 | 沈阳药科大学 | Application of folium Artemisiae Argyi in treating and preventing stomach diseases caused by helicobacter pylori |
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CN113827629A (en) * | 2021-06-07 | 2021-12-24 | 沈阳药科大学 | Application of folium Artemisiae Argyi in treating and preventing stomach diseases caused by helicobacter pylori |
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