CN106645737A - S-100 chemiluminescence immunoassay kit and preparation method thereof - Google Patents
S-100 chemiluminescence immunoassay kit and preparation method thereof Download PDFInfo
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Abstract
The invention discloses an S-100 chemiluminescence immunoassay kit and a preparation method thereof. The S-100 chemiluminescence immunoassay kit comprises an S-100 monoclonal antibody coated carboxylated magnetic particles and a statin monoclonal antibody marked chemiluminescent marker. The kit can be used for completing S-100 detection by using a full-automatic chemiluminescence immunity analyzer as a detecting tool. Experiments prove that the S-100 chemiluminescence immunoassay kit has a detection sensitivity of 0.005mu g/L, which is at least improved by 10 times in comparison with a traditional S-100 detection method. The S-100 chemiluminescence immunoassay kit has a relatively high detection accuracy.
Description
Technical field
The present invention relates to vitro detection field, more particularly to a kind of S-100 chemiluminescence immune detection reagent kits and its system
Preparation Method.
Background technology
People's S-100 albumen is concentrated mainly in the astroglia of central nervous system and corresponding tumour cell, point
Son amount is relatively low, belongs to one group of acid calbindin, because this material is soluble in the saturated ammonium sulfate solution of pH=7,
Therefore it is referred to as S-100 albumen.S-100 is most activated member in central nervous system, mainly prosperous in the snow of nervous system
In Schwann Cells and Deiter's cells, at the same exist in Langerhans cell, cartilage cell, adrenal gland satellite cell and
In the Non nervous systems such as melanic cell.
At present, clinic detects that discriminating tumour originates from using S-100 protein monoclonal antibodies to clinical samples,
Improve diagnosis of glioma correctness.The distribution of S-100 albumen is relatively broad, is currently used primarily in central nerve neuroma
Antidiastole, S-100 can as differentiate tumour be whether ectodermal origin important indicator.To in 95 astrocytomas
The expression of S-100 albumen is analyzed, and as a result shows that S-100 Protein Subtypes have expression in astrocytoma, and
Its expression is with malignancy in negative correlation.
At present the common methods of clinical detection people's S-100 albumen have enzyme linked immunosorbent assay, radio immunoassay, enzyme
Promote chemoluminescence method, but these methods all have some shortcomings part.
First, enzyme linked immunosorbent assay
Enzyme linked immunosorbent assay (ELISA) is widely used, but the method there is also following weak points：
(1) using 12 × 8 types, 6 × 8 types, 8 × 12 types or the hole Special micro porous plate of complete plate 96 as antigen coat apparatus and anti-
Container is answered, 12 batches, 6 batches, 8 batches or whole plate first use can only be divided into when in use, it is impossible to carried out independent, single
The detection of person-portion；
(2) reagent type used by quantitative determining is more, and each detection reagent will be contained with reagent bottle, and often be made
It is required for changing imbibition nozzle to be filled into respectively in the micropore of microwell plate during with a kind of reagent, not only reagent bottle species is more, filling
The operation of reagent is also extremely loaded down with trivial details；
(3) lack the corresponding mark to detection information, can only just will appreciate that by checking the mark of kit external packing box or know
The product batch number and term of validity information of detection reagent are known, and the information known is uncontrolled in detection process, with very big
Randomness；
(4) detection reagent easily causes the cross pollution between various reagents and shadow in open space in detection process
Ring the accuracy of testing result；
(5) dosage more than detection process using manual operations, reagent or sample is not bery accurate, and operating process is extremely loaded down with trivial details and multiple
It is miscellaneous, bust is susceptible to, the degree of accuracy of testing result and precision are poor；
(6) item number × 48/96 person-portion is in the quantity configuration of detection project reagent set and using on, if necessary to examine
10 projects are surveyed, then the configuration of reagent and the use of number must be 10 × 48/96 person-portions, if only a sample needs detection 10
Individual different project, it is also desirable to configure the reagent of 10 × 48/96 person-portions, haves the shortcomings that inadequate economical rationality.
2nd, radio immunoassay
Radioimmunoassay method radioactive pollution is big, load procedure is loaded down with trivial details, complex operation, operating time length, measurement result not
The stable, weak point such as the reagent holding time is short, kit operating automation degree is low, necessary instrument is expensive.
2nd, chemoluminescence method
Chemoluminescence method can be divided into direct chemiluminescence and enzyme-catalyzed chemical luminescence by principle of luminosity.
Enzyme-catalyzed chemical luminescence mainly has horseradish peroxidase（HRP）With two kinds of alkaline phosphatase, but there is certain office
Sex-limited, horseradish peroxidase major defect is：Luminol, also can be by H in the case of existing without horseradish peroxidase2O2
Oxidation itself lights, and background is of a relatively high, affects signal to noise ratio, and kinetics is complicated, and influence factor is more, is as a result not sufficiently stable,
The substrate for obtaining sensitivity height and plateau length is not easy.Alkaline phosphatase major defect is：Substrate reach plateau when
Between long, substrate high cost, cause testing cost high, patient burden's weight.
Acridinium ester compares enzyme-catalyzed chemical luminescence and has detailed advantage, main performance as the direct chemiluminescence of label
：Reaction does not need catalyst, as long as alkaline environment can be carried out, is swift in response, and background luminescence is low, and signal to noise ratio is high, disturb because
Element is few, and reagent stability is good, can be simple with two-point calibration, system, exciting liquid low cost, acridinium ester easily and protein bind, and
Photon yield is not reduced after connection.
The content of the invention
Based on this, it is necessary to provide a kind of higher S-100 chemiluminescence immune detection reagent kits of detection sensitivity and its
Preparation method.
A kind of S-100 chemiluminescence immune detection reagent kits, including：The coated carboxylated of S-100 monoclonal antibodies
The chemiluminescent labels of magnetic particle and inhibin labeling of monoclonal antibody.
In one embodiment, in the magnetic particle of the coated carboxylated of the S-100 monoclonal antibodies, the S-100
The ratio of the magnetic particle of monoclonal antibody and the carboxylated is 1：25~35.
In one embodiment, in the chemiluminescent labels of the inhibin labeling of monoclonal antibody, the S-100
The ratio of monoclonal antibody and the chemiluminescent labels is 50：1~10.
In one embodiment, the particle diameter of the magnetic particle of the carboxylated is 0.05 μm ~ 1 μm.
In one embodiment, the chemiluminescent labels are luminol, different luminol, tris (bipyridine) ruthenium or acridine
Ester.
In one embodiment, also including Chemoluminescent substrate, the Chemoluminescent substrate includes A liquid and B liquid.
In one embodiment, the A liquid is H2O2Solution, the B liquid is NaOH solution.
In one embodiment, also product are calibrated including S-100.
In one embodiment, the S-100 calibrations product are respectively 00 μ g/L, 0.01 μ g/L, 0.2 μ g/L, 3 μ for concentration
The solution of the S-100 of g/L, 10 μ g/L and 40 μ g/L.
A kind of preparation method of above-mentioned S-100 chemiluminescence immune detection reagent kits, comprises the steps：
The suspension of the magnetic particle of carboxylated is taken, Magneto separate goes after supernatant to use MES buffer solutions resuspended, is subsequently added into the EDC aqueous solution,
The surface carboxyl groups of the magnetic particle of activated carboxyl, are subsequently added into S-100 monoclonal antibodies, suspension 2h ~ 10h, Magneto separate under room temperature
It is resuspended with Tris buffer solutions after removal supernatant, obtain the magnetic particle of the coated carboxylated of S-100 monoclonal antibodies；And
S-100 monoclonal antibodies are taken, is added and mixed after carbonate buffer solution, be subsequently adding after chemiluminescent labels and mix, room
Removal of impurities after the lower lucifuge reaction 1h ~ 2h of temperature, the chemiluminescent labels of the plain labeling of monoclonal antibody that is inhibited.
This S-100 chemiluminescence immune detection reagent kits can be with Full-automatic chemiluminescence immunoassay analysis meter as detection
Instrument, completes this S-100 chemiluminescence immune detection reagent kits of detection of S-100, and through experiment, its detection sensitivity reaches
To 0.05 μ g/L, the detection method sensitivity relative to traditional S-100 at least improves 10 times, and this S-100 chemistry is sent out
The accuracy of detection of light immunity detection reagent is higher.
Description of the drawings
Fig. 1 is the flow chart of the preparation method of the S-100 chemiluminescence immune detection reagent kits of an embodiment；
Fig. 2 is the S-100 canonical plottings that embodiment 3 is obtained.
Specific embodiment
It is understandable to enable the above objects, features and advantages of the present invention to become apparent from, it is below in conjunction with the accompanying drawings and concrete real
Apply example to be described in detail the specific embodiment of the present invention.Elaborate many details in the following description in order to
Fully understand the present invention.But the present invention can be implemented with being much different from alternate manner described here, art technology
Personnel can do similar improvement in the case of without prejudice to intension of the present invention, therefore the present invention is not embodied as by following public
Restriction.
The S-100 chemiluminescence immune detection reagent kits of one embodiment, including：S-100 monoclonal antibodies are coated
The magnetic particle of carboxylated and the chemiluminescent labels of inhibin labeling of monoclonal antibody.
Preferably, in the magnetic particle of the coated carboxylated of S-100 monoclonal antibodies, S-100 monoclonal antibodies and carboxyl
The ratio of the magnetic particle of change is 1：25~35.
Preferably, in the chemiluminescent labels of inhibin labeling of monoclonal antibody, S-100 monoclonal antibodies with chemistry
The ratio of luminous marker is 50：1~10.
Preferably, the particle diameter of the magnetic particle of carboxylated is 0.05 μm ~ 1 μm.
Chemiluminescent labels can be luminol, different luminol, tris (bipyridine) ruthenium or acridinium ester.Wherein, chemiluminescence
Label is preferably acridinium ester.
In other examples, above-mentioned S-100 chemiluminescence immune detection reagent kits also include chemical luminous substrate
Liquid.
Chemoluminescent substrate includes A liquid and B liquid.A liquid can be H2O2Solution, B liquid can be NaOH solution.
In the present embodiment, A liquid is the H that concentration is 0.1mol/L2O2Solution, B liquid is that concentration is molten for the NaOH of 0.25mol/L
Liquid.
In other examples, above-mentioned S-100 chemiluminescence immune detection reagent kits also include that S-100 calibrates product.
S-100 calibration product are respectively 0 μ g/L, 0.01 μ g/L, 0.2 μ g/L, 3 μ g/L, 10 μ g/L and 40 μ g/L for concentration
The solution of S-100.
Specifically, S-100 calibration product can using standard items buffer solution by S-100 be configured to concentration be respectively 0 μ g/L,
The solution of the S-100 of 0.01 μ g/L, 0.2 μ g/L, 3 μ g/L, 10 μ g/L and 40 μ g/L.
When this S-100 chemiluminescence immune detection reagent kits are detected for S-100, exempted from using Full-automatic chemiluminescence
Epidemic disease analyzer detects that drafting calibration curve is built in computer software to S-100 calibration product；Then actual sample is tested,
Concentration of specimens is calculated according to sample luminous value；Finally performance is carried out to S-100 automatic chemiluminescence immunoassays system（Spirit
Sensitivity, linear, precision, interference）Evaluation.
This S-100 chemiluminescence immune detection reagent kits can be with Full-automatic chemiluminescence immunoassay analysis meter as detection
Instrument, completes this S-100 chemiluminescence immune detection reagent kits of detection of S-100, and through experiment, its detection sensitivity reaches
To 0.005 μ g/L, the detection method sensitivity relative to traditional S-100 at least improves 10 times, and this S-100 chemistry is sent out
The accuracy of detection of light immunity detection reagent is higher.
Additionally, this S-100 chemiluminescence immune detection reagent kits have further the advantage that：
1st, acridinium ester being selected as marker material, and being applied to chemiluminescence immunoassay system, the luminescence system is directly change
Learn luminous, compared with traditional enzyme-catalyzed chemical luminescence, the reaction does not need the participation of enzyme, more cost-effective；
2nd, from the chemiluminescence immunoassay system range of linearity width of acridinium ester, 0.005 μ g/L ~ 40 μ g/L can be reached, and is passed
The inspection range of linearity of the detection method of the S-100 of system is 0.05 μ g/L ~ 10 μ g/L；
3rd, acridinium ester chemiluminescent immunoassay system repeatability is high, and within 5%, this is other chemistry to batch interior and difference between batch
Luminescence immunoassay system is unapproachable；
4th, chemiluminescence immunoassay system has realized the quantitative of sample, by built-in calibration curve to test software, only needs to survey
Sample originally can directly obtain the concentration value of sample；
5th, chemiluminescence immunoassay system can realize that the addition of full-automation, reagent and sample has instrument to complete entirely, operation
It is easier, reduce artificial error.
The preparation method of above-mentioned S-100 chemiluminescence immune detection reagent kits as shown in Figure 1, comprises the steps：
The suspension of the magnetic particle of carboxylated is taken, Magneto separate goes after supernatant to use MES buffer solutions resuspended, is subsequently added into the EDC aqueous solution,
The surface carboxyl groups of the magnetic particle of activated carboxyl, are subsequently added into S-100 monoclonal antibodies, suspension 2h ~ 10h, Magneto separate under room temperature
It is resuspended with Tris buffer solutions after removal supernatant, obtain the magnetic particle of the coated carboxylated of S-100 monoclonal antibodies.
MES（2- (N- morpholines) ethyl sulfonic acid）The concentration of buffer solution is 0.02M, and pH is 5.5.
The concentration of Tris buffer solutions is 0.1M and contains 2%BSA, and pH is 8.0.
EDC（1- ethyl -3- (3- dimethyl aminopropyls)-carbodiimides）The concentration of the aqueous solution is 10mg/mL ~ 20mg/
The ratio of the magnetic particle of mL, EDC and carboxylated is 0.05：0.1~1.
Preferably, in the magnetic particle of the coated carboxylated of S-100 monoclonal antibodies, S-100 monoclonal antibodies and carboxyl
The ratio of the magnetic particle of change is 1：25~35.
Preferably, the particle diameter of the magnetic particle of carboxylated is 0.05 μm ~ 1 μm.
S-100 monoclonal antibodies are taken, is added and mixed after carbonate buffer solution, be subsequently adding after chemiluminescent labels and mix
It is even, removal of impurities after lucifuge reaction 1h ~ 2h, the chemiluminescent labels of the plain labeling of monoclonal antibody that is inhibited under room temperature.
Carbonate buffer solution concentration is 0.1M, and pH is 9.0 ~ 9.5,
The operation of removal of impurities is centrifugation desalting column desalination, and concrete operations are：First respectively with pure water and TBS buffer solutions（40 mM
Tris-HCl, 0.5% BSA, 1% NaCl, pH 8.0）Centrifugation desalting column is processed, the S-100 monoclonals for obtaining is eventually adding and is resisted
The solution of the magnetic particle of the coated carboxylated of body, finally collects the liquid in centrifuge tube.
Preferably, in the chemiluminescent labels of inhibin labeling of monoclonal antibody, S-100 monoclonal antibodies with chemistry
The ratio of luminous marker is 50：1~10.
Chemiluminescent labels can be luminol, different luminol, tris (bipyridine) ruthenium or acridinium ester.Wherein, chemiluminescence
Label is preferably acridinium ester.
The magnetic particle of the coated carboxylated of S-100 monoclonal antibodies for obtaining and the change of inhibin labeling of monoclonal antibody
Learn luminous marker combination and above-mentioned S-100 chemiluminescence immune detection reagent kits are obtained.
This S-100 chemiluminescence immune detection reagent kits are when in use, in addition it is also necessary to Chemoluminescent substrate and S-100
Calibration product.
Chemoluminescent substrate and S-100 calibration product can voluntarily be prepared and obtained.
Chemoluminescent substrate includes A liquid and B liquid.A liquid can be H2O2Solution, B liquid can be NaOH solution.
In the present embodiment, A liquid is the H that concentration is 0.1mol/L2O2Solution, B liquid is that concentration is molten for the NaOH of 0.25mol/L
Liquid.
Specifically, S-100 calibration product can using standard items buffer solution by S-100 be configured to concentration be respectively 0 μ g/L,
The solution of the S-100 of 0.01 μ g/L, 0.2 μ g/L, 3 μ g/L, 10 μ g/L and 40 μ g/L.
The preparation method of this S-100 chemiluminescence immune detection reagent kits is simple and convenient, and obtained S-100 chemistry is sent out
The detection sensitivity of light immunity detection reagent is higher, has a good application prospect.
It is below specific embodiment.
Embodiment 1：The preparation of S-100 chemiluminescence immune detection reagent kits
（1）The preparation of the magnetic particle of the coated carboxylated of S-100 monoclonal antibodies：
Take the magnetic particle for 0.05 μm ~ 1 μm of carboxylated containing 50mg particle diameters（MagnaBind21353）Suspension, Magneto separate goes
Supernatant, uses 0.02 M, pH to be that 5.5 MES buffer solutions are resuspended, adds the EDC aqueous solution of the 10mg/mL of the new configurations of 1mL, activates magnetic
Bead surface carboxyl, adds 4mgS-100 monoclonal antibodies（Biorbyt, article No. orb48780）, be suspended 6h under room temperature, Magneto separate,
Supernatant is removed, with the 0.1M containing 2%BSA, pH is that 8.0 Tris buffer solutions are resuspended to 1mg/mL, obtains S-100 monoclonal antibodies
The magnetic particle of coated carboxylated, every bottle of 5mL packing be stored in 4 DEG C it is standby.
（2）The preparation of the acridinium ester of inhibin labeling of monoclonal antibody：
The S-100 monoclonal antibodies that 50 μ L concentration are 25mg/mL are taken, the carbon that 150 μ L concentration are that 0.1M, pH are 9.0 ~ 9.5 is added
Phthalate buffer, mixes, and is subsequently adding the acridine ester solution that 1.5 μ L concentration are 5mg/mL and mixes, lucifuge reaction, 1.5h under room temperature
After take out, desalting column desalting processing is centrifuged with the zeba of 2mL, entered with pure water and TBS buffer solutions respectively first in desalination processes
Row is processed, and is eventually adding the acridine ester solution of the inhibin labeling of monoclonal antibody for obtaining, and collects the liquid in centrifuge tube to guarantor
Deposit the acridinium ester for being in control inhibin labeling of monoclonal antibody, every bottle of 5mL packing be stored in 4 DEG C it is standby.
（3）S-100 calibrates the preparation of product：
Use standard items buffer solution（40 mM Tris-HCl, 0.5% BSA, 1% NaCl, pH 8.0）S-100 is configured to into concentration
For 0 μ g/L, 0.01 μ g/L, 0.2 μ g/L, 3 μ g/L, 10 μ g/L and 40 μ g/L, per bottle of 0.5 mL packing is lyophilized, and 4 DEG C of preservations are standby
With.
Embodiment 2：S-100 chemical luminous immune detection methods
With Full-automatic chemiluminescence immunoassay analysis meter（YHLO, article No. iFlash3000）To detect instrument, methodology pattern is double
Antibody sandwich, i.e. instrument sequentially add the sample of 50 μ L, the magnetic of the coated carboxylated of S-100 monoclonal antibodies of 50 μ L
The S-100 treatment fluids of particulate and 50 μ L, reaction 20 min after, then add 50 μ L the coated acridinium esters of S-100, reaction 20
After min, Magneto separate is carried out, reactant mixture is sent into darkroom by instrument, sequentially add luminous substrate A liquid（H2O2）And B liquid（NaOH）
Luminescence-producing reaction is carried out, luminous value is finally recorded.
Embodiment 3：S-100 chemiluminescence immune detection reagent kit performance evaluations
S-100 calibration product are detected using the method in embodiment 2, obtains drafting calibration curve as shown in Figure 2.
Then to then testing actual sample, concentration of specimens is calculated according to sample luminous value.
The detection of sensitivity：
With reference to CLSI EP17-A file recommendation experimental programs, the sensitive of S-100 chemiluminescence immune detection reagent kits is calculated
Degree, the sensitivity tried to achieve is 10pg/ mL.
Linear detection：
Linear analysis is done for 0 μ g/L, 0.01 μ g/L, 0.2 μ g/L, 3 μ g/L, 10 μ g/L and 40 μ g/L standard items to concentration, is calculated
Linearly dependent coefficient, r=0.9996, in addition, the kit is 10 μ g/L ~ 1300 μ to the range of linearity of S-100 sample detections
g/L。
Precision is determined：
Concentration is taken for two S-100 samples of 0.2 μ g/L and 20 μ g/L, each sample each concentration respectively do 3 it is parallel, with three batches
Kit is detected, calculated in kit batch and difference between batch, as a result shown in the kit batch and difference between batch is respectively less than 5%.
Interference is tested：
Taking pooled serum and adding chaff interference respectively includes：Combined with bilirubin, unconjugated bilirubin, hemoglobin, ascorbic acid, glycerine
Ester, adding proportion is according to 1：20 are carried out, and are determined pooled serum respectively and be with the addition of the measured value of pooled serum after various chaff interferences,
Deviation therebetween is calculated, with ± 10% as tolerance interval.As a result show, interference reaches the files-designated of NCCLS
Standard, can be used for the accurate evaluation of clinical labororatory's S-100 situations.
The contrast experiment of embodiment 4, S-100 chemiluminescence immune detection reagent kits
It is respectively 0 μ g/L, 0.01 μ g/L, 0.2 μ g/ to concentration with chemical luminescence detection method and traditional enzyme linked immunosorbent assay
The S-100 samples of L, 3 μ g/L, 10 μ g/L and 40 μ g/L detect that two methods detection sensitivity is compared, data such as following table institute
Show：
As can be seen from the above table, the sensitivity of chemical luminescence detection method improves about 20 times compared with enzyme linked immunosorbent assay.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more concrete and detailed, but can not
Therefore it is interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art,
Without departing from the inventive concept of the premise, some deformations and improvement can also be made, these belong to the protection model of the present invention
Enclose.Therefore, the protection domain of patent of the present invention should be defined by claims.
Claims (10)
1. a kind of S-100 chemiluminescence immune detection reagent kits, it is characterised in that include：S-100 monoclonal antibodies are coated
The magnetic particle of carboxylated and the chemiluminescent labels of inhibin labeling of monoclonal antibody.
2. S-100 chemiluminescence immune detection reagent kits according to claim 1, it is characterised in that the S-100 is mono-
In the magnetic particle of the coated carboxylated of clonal antibody, the ratio of the magnetic particle of the S-100 monoclonal antibodies and the carboxylated
For 1：25~35.
3. S-100 chemiluminescence immune detection reagent kits according to claim 1, it is characterised in that the inhibin list
In the chemiluminescent labels of clonal antibody mark, the ratio of the S-100 monoclonal antibodies and the chemiluminescent labels
For 50：1~10.
4. S-100 chemiluminescence immune detection reagent kits according to claim 1, it is characterised in that the carboxylated
The particle diameter of magnetic particle is 0.05 μm ~ 1 μm.
5. S-100 chemiluminescence immune detection reagent kits according to claim 1, it is characterised in that the chemiluminescence
Label is luminol, different luminol, tris (bipyridine) ruthenium or acridinium ester.
6. S-100 chemiluminescence immune detection reagent kits according to claim 1, it is characterised in that also send out including chemistry
Light substrate solution, the Chemoluminescent substrate includes A liquid and B liquid.
7. S-100 chemiluminescence immune detection reagent kits according to claim 6, it is characterised in that the A liquid is H2O2
Solution, the B liquid is NaOH solution.
8. S-100 chemiluminescence immune detection reagent kits according to claim 1, it is characterised in that also including S-100
Calibration product.
9. S-100 chemiluminescence immune detection reagent kits according to claim 8, it is characterised in that the S-100 determines
Mark product are respectively the solution of the S-100 of 0 μ g/L, 0.01 μ g/L, 0.2 μ g/L, 3 μ g/L, 10 μ g/L and 40 μ g/L for concentration.
10. the preparation side of S-100 chemiluminescence immune detection reagent kit of the one kind according to any one of claim 1 ~ 9
Method, it is characterised in that comprise the steps：
The suspension of the magnetic particle of carboxylated is taken, Magneto separate goes after supernatant to use MES buffer solutions resuspended, is subsequently added into the EDC aqueous solution,
The surface carboxyl groups of the magnetic particle of activated carboxyl, are subsequently added into S-100 monoclonal antibodies, suspension 2h ~ 10h, Magneto separate under room temperature
It is resuspended with Tris buffer solutions after removal supernatant, obtain the magnetic particle of the coated carboxylated of S-100 monoclonal antibodies；And take S-
100 monoclonal antibodies, add and mixed after carbonate buffer solution, are subsequently adding after chemiluminescent labels and mix, lucifuge under room temperature
Removal of impurities after reaction 1h ~ 2h, the chemiluminescent labels of the plain labeling of monoclonal antibody that is inhibited.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610503817.XA CN106645737A (en) | 2016-06-30 | 2016-06-30 | S-100 chemiluminescence immunoassay kit and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610503817.XA CN106645737A (en) | 2016-06-30 | 2016-06-30 | S-100 chemiluminescence immunoassay kit and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109633162A (en) * | 2018-11-28 | 2019-04-16 | 浙江聚康生物工程有限公司 | S-100 β protein detection kit |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102331502A (en) * | 2011-08-31 | 2012-01-25 | 内蒙古科慧生物科技有限责任公司 | Quantitative measurement kit and detection method for human S100 protein (S-100) |
| CN104655855A (en) * | 2015-01-23 | 2015-05-27 | 宁波大学 | Preparation method and application of tumor marker electrochemiluminescence immunoassay sensor based on multifunctional carbon nitride material |
-
2016
- 2016-06-30 CN CN201610503817.XA patent/CN106645737A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102331502A (en) * | 2011-08-31 | 2012-01-25 | 内蒙古科慧生物科技有限责任公司 | Quantitative measurement kit and detection method for human S100 protein (S-100) |
| CN104655855A (en) * | 2015-01-23 | 2015-05-27 | 宁波大学 | Preparation method and application of tumor marker electrochemiluminescence immunoassay sensor based on multifunctional carbon nitride material |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109633162A (en) * | 2018-11-28 | 2019-04-16 | 浙江聚康生物工程有限公司 | S-100 β protein detection kit |
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Application publication date: 20170510 |