CN106645702A - Rapid detection device for genetically modified ingredients in grain and oils - Google Patents
Rapid detection device for genetically modified ingredients in grain and oils Download PDFInfo
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- CN106645702A CN106645702A CN201710064520.2A CN201710064520A CN106645702A CN 106645702 A CN106645702 A CN 106645702A CN 201710064520 A CN201710064520 A CN 201710064520A CN 106645702 A CN106645702 A CN 106645702A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
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Abstract
The invention specifically discloses a rapid detection device for genetically modified ingredients in grain and oils. The rapid detection device comprises a sample feeding device, a plurality of hollow cellulose polypropylene membranes and a porous plate body, wherein primary antibodies, which are specifically combined with genetically modified proteins in total proteins, are attached to the hollow cellulose polypropylene membranes, so that the genetically modified proteins are combined with the primary antibodies and are attached onto the hollow cellulose polypropylene membranes. The rapid detection device disclosed by the invention is simple in design, convenient to use and easy to operate. According to the rapid detection device disclosed by the invention, the antibodies of genetically modified expression proteins are fixed on the cellulose polypropylene membranes, so that whether the genetically modified proteins exist in raw materials or not is detected through enabling the total proteins of raw materials to be detected to flow through the detection device, and whether the genetically modified proteins exist or not can be effectively detected. The rapid detection device is provided with two sample feeding holes at the same time and two groups of the hollow cellulose polypropylene membranes are arranged, so that the device can be used for detecting two groups of samples at the same time.
Description
Technical field
The present invention relates to food transgene component rapid detection technical field, in particular it relates to transgenosis in a kind of grain and oil
The device for fast detecting of composition.
Background technology
In order to strengthen the ability of crop insect pest, antiviral etc.；Agricultural product storage property is improved, increases crop yield.Turn base
Because crop is the new focus in present breeding field, one or more allogenic genes are transferred to by certain by technique for gene engineering
In specific organism, and it is set effectively to give expression to corresponding product (polypeptide or protein), this process is transgenosis.Turn base
Because biological safety evaluation is the premise of genetically modified organism and products thereof marketization and commercialization, in safety evaluation transgenosis into
Point detection be an important content, set up that genetically modified organism is quick, easy, accurate detection method is safety evaluation and management
Lay the foundation.
The detection of vegetalitas GM food mainly adopts following two technology paths, and one is using PCR, Southern etc.
The foreign gene of technology for detection insertion, two is the albumen for using the detection transgene expression such as Elisa, Western.Protein level
Detection, the immunological method that antigen and antibody interact is mainly based upon, for external source in genetically modified plants and products thereof
The specific proteins of destination gene expression carries out qualitative and quantitative determination, and its result is relatively more accurate, and reliability is also more preferable.But
It is that current detection means is substantially molecular biology method, it usually needs the laboratory that dependence possesses good experiment condition is entered
OK, and complex steps, it usually needs the experimenter of specialty is operated, obtain experimental result process and compare man, it is impossible to be full
Enough some need the mechanism of Rapid Detection transgene component.
The content of the invention
For the deficiency in prior art, the present invention provides a kind of device for fast detecting of transgene component in grain and oil.
In order to solve above-mentioned technical problem, the present invention is achieved by the following technical programs：
The device for fast detecting of transgene component in a kind of grain and oil, including sample adding device, many hollow cellulose polypropylene
Film and porous plate body, are attached with and the transgenic protein specific binding in total protein on the doughnut polypropylene screen
One resists, and the sample adding device includes the 2nd porous plate body, and the cone of a formed cavity, institute are provided with the 2nd porous plate body
State cone diameter to be gradually reduced from down to up, one end of many doughnut polypropylene screens is fixed on the 2nd porous
Plate body and connect with the hole on the 2nd porous plate body, the other end of many doughnut polypropylene screens is fixed on institute
State the 1st porous plate body and connect with the hole on the 1st porous plate body, when total protein is by the detection means, closing
Hole on the 1st porous plate body, so that the flowing of total protein occurs over just the one of many doughnut polypropylene screens
Between end and the fenestra of the doughnut polypropylene screen, make described in the transgenic protein merges with a resistive connection and be attached to
On doughnut polypropylene screen.
Relative to prior art, beneficial effects of the present invention：
1st, present invention design is simple, easy to use, it is easy to operate.
2nd, the antibody of transgene expression albumen is creatively fixed on fiber polypropylene film by the present invention, so as to pass through to make to treat
The total protein of detection raw material whether there is transgenic protein in flowing through the detection means to detect raw material, can be effectively fast
Detect whether there is transgene protein fastly.
3rd, the present invention arranges two wells simultaneously, two groups of doughnut polypropylene screens, thus the device of the present invention can be with
Carry out two groups of sample detections simultaneously.
4th, device of the invention detection transgenic product, the degree of accuracy is higher, reaches 96% or so, although be also not reaching to glimmering
The degree of accuracy of Fluorescent Quantitative PCR, but the detection of the detection means of the present invention is quick, and detecting step is easy, time-consuming short, for precision will
Seeking the device of not high field quick detection, the present invention can meet completely.It is inclined that the detection means of the present invention is detected for its 10 times
Rate shows preferable using the device testing result stability of the present invention 7% or so, and its testing result has more objectivity.
Description of the drawings
Accompanying drawing herein is merged in specification and constitutes the part of this specification, shows the enforcement for meeting the present invention
Example, and be used to explain the principle of the present invention together with specification.
Fig. 1 is the structural representation of the present invention.
Specific embodiment
With the following Examples the invention will be further described.
Fig. 1 is the structural representation of the device for fast detecting of transgene component in a kind of grain and oil shown in exemplary embodiment
Figure, as shown in Figure 1：
The device for fast detecting of transgene component in a kind of grain and oil, including sample adding device, many hollow cellulose polypropylene
Film and porous plate body, are attached with and the transgenic protein specific binding in total protein on the doughnut polypropylene screen
One resists, and the sample adding device includes the 2nd porous plate body, and the cone of a formed cavity, institute are provided with the 2nd porous plate body
State cone diameter to be gradually reduced from down to up, one end of many doughnut polypropylene screens is fixed on the 2nd porous
Plate body and connect with the hole on the 2nd porous plate body, the other end of many doughnut polypropylene screens is fixed on institute
State the 1st porous plate body and connect with the hole on the 1st porous plate body, when total protein is by the detection means, closing
Hole on the 1st porous plate body, so that the flowing of total protein occurs over just the one of many doughnut polypropylene screens
Between end and the fenestra of the doughnut polypropylene screen, make described in the transgenic protein merges with a resistive connection and be attached to
On doughnut polypropylene screen.
Preferably, the cone arranges 2, and each cone arranges one group of corresponding doughnut polypropylene
Film, each group of doughnut polypropylene screen is independently arranged, and detects multiple testing samples simultaneously on one side.
Preferably, each group of doughnut polypropylene screen arranges 5-15 roots, the long 10-20cm per root；Every doughnut gathers
Propylene film oneself is formed with a chamber, a diameter of 10～20mm of every doughnut polypropylene screen.
It is highly preferred that in the above grain and oil transgene component device for fast detecting, can be used for corn, paddy rice, height
The transgene component quick detection of fine strain of millet, wheat and soybean, it is also possible to for the processed goods of corn, paddy rice, Chinese sorghum, wheat and soybean
Transgene component quick detection.
The antibody of transgene expression albumen is creatively fixed on fiber polypropylene film by the present invention, so as to be checked by making
The total protein of survey raw material whether there is transgenic protein in flowing through the detection means to detect raw material, can be effectively quick
Detect whether there is transgene protein.Two wells of setting simultaneously of the invention, two groups of doughnut polypropylene screens, thus this
The device of invention can simultaneously carry out two groups of sample detections.
Introduce to following exemplary a kind of quick detection of transgene component in grain and oil of the detection means using the present invention
Method, comprises the following steps：
S1. the gross protein of testing sample is extracted；
S2. the gross protein injection well for S1 being obtained, closes the hole on the first porous plate body, so that total egg
White flowing occur over just one end of many doughnut polypropylene screens and the doughnut polypropylene screen fenestra it
Between, the transgenic protein is made with an anti-binding and then be attached on the doughnut polypropylene screen；
S3 opens the hole on the first porous plate body, washs the residue in the chamber of the doughnut polypropylene screen；
S4 closes again the hole on the first porous plate body, by specifically binding with the transgenic protein and
With the markd two anti-wells for being expelled to the detection means, two are made to resist by the detection means；
S5. the hole being again turned on the first porous plate body, in washing the chamber of the doughnut polypropylene screen
Residue；
S6. detected using DAB horseradish peroxidase colour reagents box and be whether there is on the doughnut polypropylene screen
Described two anti-marks, if there is the mark, then it is assumed that there is the transgene protein in the total protein, and judge the raw material
In there is transgene component.
The device for fast detecting of transgene component in grain and oil of the present invention, during the first antibody of transgenic protein is attached to
On hollow fiber polypropylene screen, so as to be in by making the total protein of raw material to be detected and flowing through the detection means to detect raw material
It is no to there is transgenic protein, and protein fluid is added in the detection means of the present invention by syringe, it is easy to grasp
Make, the device of the present invention can effectively and rapidly detect whether there is transgene protein, can quickly examine according to the method for the present invention
Transgene protein is surveyed, the method for the present invention is quick for the detection of transgene protein, accurate, efficiency high.
Experimental example
Due to the concept is that combined with its antibody with transgene product protein, and then detect whether to exist outer
Source gene.In order to verify the feasibility and accuracy and stability of the present invention, this experimental example is simultaneously using in higher sensitivity glimmering
Fluorescent Quantitative PCR is verified.Same testing sample is taken, is detected using the device and quantitative fluorescent PCR of the present invention respectively,
Apparatus of the present invention are experimental group, and quantitative fluorescent PCR is control group, and each process is repeated 3 times, and results averaged is calculated, as a result
As shown in table 1：
Table 1
| Sample | The experimental group degree of accuracy | The control group degree of accuracy | 10 deviation ratios |
| Flour | 95.7% | 99.3% | 5.9% |
| Rice | 96.5% | 99.1% | 7.1% |
| Corn | 97.2% | 98.9% | 6.5% |
Consolidated statement 1 can be known, using the device detection transgenic product of the present invention, the degree of accuracy is higher, reaches 96% or so,
Although being also not reaching to the degree of accuracy of quantitative fluorescent PCR, the detection means detection of the present invention is quick, and detecting step is easy,
Time-consuming short, not high for required precision field quick detection, the device of the present invention can meet completely.The detection dress of the present invention
The deviation ratio of its 10 times detections is put 7% or so, shows preferable using the device testing result stability of the present invention, its detection knot
Fruit has more objectivity.
Finally it should be noted that above example is only illustrating technical scheme, rather than to present invention guarantor
The restriction of shield scope, although having made to explain to the present invention with reference to preferred embodiment, one of ordinary skill in the art should
Work as understanding, technical scheme can be modified or equivalent, without deviating from the reality of technical solution of the present invention
Matter and scope.
Claims (3)
1. in a kind of grain and oil transgene component device for fast detecting, it is characterised in that including sample adding device, many doughnuts
Plain polypropylene screen and porous plate body, are attached with special with the transgenic protein in total protein on the doughnut polypropylene screen
Property combine one resist, the sample adding device include the 2nd porous plate body, a formed cavity is provided with the 2nd porous plate body
Cone, the cone diameter is gradually reduced from down to up, and one end of many doughnut polypropylene screens is fixed on institute
State the 2nd porous plate body and connect with the hole on the 2nd porous plate body, the other end of many doughnut polypropylene screens
It is fixed on the 1st porous plate body and connects with the hole on the 1st porous plate body, when total protein is by the detection means
When, the hole on the 1st porous plate body is closed, so that the flowing of total protein occurs over just many doughnut polypropylene
Between the fenestra of one end of film and the doughnut polypropylene screen, the transgenic protein is set to merge attachment with a resistive connection
To on the doughnut polypropylene screen.
2. in grain and oil according to claim 1 transgene component device for fast detecting, it is characterised in that the cone
2 are arranged, each cone arranges one group of corresponding doughnut polypropylene screen, each group of doughnut polypropylene screen is only
Erect and put, detect multiple testing samples simultaneously on one side.
3. in grain and oil according to claim 2 transgene component device for fast detecting, it is characterised in that each group is hollow
Fiber polypropylene film arranges 5-15 roots, the long 10-20cm per root；Every doughnut polypropylene screen oneself is formed with a chamber, often
A diameter of 10～20mm of root doughnut polypropylene screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710064520.2A CN106645702A (en) | 2017-02-04 | 2017-02-04 | Rapid detection device for genetically modified ingredients in grain and oils |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710064520.2A CN106645702A (en) | 2017-02-04 | 2017-02-04 | Rapid detection device for genetically modified ingredients in grain and oils |
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| Publication Number | Publication Date |
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| CN106645702A true CN106645702A (en) | 2017-05-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710064520.2A Pending CN106645702A (en) | 2017-02-04 | 2017-02-04 | Rapid detection device for genetically modified ingredients in grain and oils |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1438484A (en) * | 2003-03-25 | 2003-08-27 | 江苏省农业科学院经济作物研究所 | Detection of transfer Bt gene anti-pest cotton by gold marking immuno-adsorption method |
| US20090133161A1 (en) * | 2007-11-20 | 2009-05-21 | Pioneer Hi-Bred International, Inc. | Maize Ethylene Signaling Genes and Modulation of Same for Improved Stress Tolerance in Plants |
| CN102645535A (en) * | 2012-04-12 | 2012-08-22 | 北京奥瑞金种业股份有限公司 | Method for detecting roundup ready protein G2-aroA and special enzyme linked immune kit |
| CN104020296A (en) * | 2014-06-09 | 2014-09-03 | 无锡福阳生物科技有限公司 | Quantitative detection method of transgenosis protein CP4EPSPS |
| CN104569432A (en) * | 2015-01-06 | 2015-04-29 | 通标标准技术服务有限公司 | Method and device for detecting transgenic ingredients in food |
| CN106324258A (en) * | 2016-08-29 | 2017-01-11 | 黎志春 | Auxiliary method for detecting whether genetically modified protein exists in food |
-
2017
- 2017-02-04 CN CN201710064520.2A patent/CN106645702A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1438484A (en) * | 2003-03-25 | 2003-08-27 | 江苏省农业科学院经济作物研究所 | Detection of transfer Bt gene anti-pest cotton by gold marking immuno-adsorption method |
| US20090133161A1 (en) * | 2007-11-20 | 2009-05-21 | Pioneer Hi-Bred International, Inc. | Maize Ethylene Signaling Genes and Modulation of Same for Improved Stress Tolerance in Plants |
| CN102645535A (en) * | 2012-04-12 | 2012-08-22 | 北京奥瑞金种业股份有限公司 | Method for detecting roundup ready protein G2-aroA and special enzyme linked immune kit |
| CN104020296A (en) * | 2014-06-09 | 2014-09-03 | 无锡福阳生物科技有限公司 | Quantitative detection method of transgenosis protein CP4EPSPS |
| CN104569432A (en) * | 2015-01-06 | 2015-04-29 | 通标标准技术服务有限公司 | Method and device for detecting transgenic ingredients in food |
| CN106324258A (en) * | 2016-08-29 | 2017-01-11 | 黎志春 | Auxiliary method for detecting whether genetically modified protein exists in food |
Non-Patent Citations (1)
| Title |
|---|
| 鲍时翔等: "A蛋白中空纤维膜吸附分离单克隆抗体", 《膜科学与技术》 * |
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