CN106645154A - Enrichment and observation apparatus and method for parasite eggs - Google Patents
Enrichment and observation apparatus and method for parasite eggs Download PDFInfo
- Publication number
- CN106645154A CN106645154A CN201611209149.6A CN201611209149A CN106645154A CN 106645154 A CN106645154 A CN 106645154A CN 201611209149 A CN201611209149 A CN 201611209149A CN 106645154 A CN106645154 A CN 106645154A
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- China
- Prior art keywords
- substrate
- enrichment
- thin film
- film chip
- finder
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides an enrichment and observation apparatus and method for parasite eggs; the apparatus comprises a film chip and a sliding table; the film chip is composed of a substrate and transparent film with the edge sealed to the substrate, a liquid storage cavity is formed between the transparent film and the substrate, a guide post is arranged in the sliding table, a slider is mounted on the guide post, a handle is arranged above the slider, a slide plate is arranged below the slider, a distance from the bottom end of the slide plate to the substrate is less than a distance from the highest point of the liquid storage cavity to the substrate, the film chip is provided with adjustable height support legs, a sample under test is mixed well by moving the slider side to side, the sample then binds specifically to parasite eggs through magnetic beads, the parasite eggs are enriched and purified on the chip under the action of a magnetic field, and subsequent microscope detection is facilitated. The apparatus and method are applicable to the enrichment of parasite eggs from discharges such as excrement and urine; by using the apparatus and method, the specific enrichment for parasite eggs is achieved, and the apparatus and method are directly applicable to microscopic observation, simple to perform and easy to automate.
Description
Technical field
The present invention relates to Bioexperiment equipment technical field, more particularly, to a kind of enrichment observation of parasite egg
Apparatus and method.
Background technology
In field of parasitosis diagnosis, the goldstandard of its clinical diagnosis is extracted simultaneously from host's body or in host's excreta
It was found that parasite egg.For example to wreaking havoc the detection of the Japanese schistosomiasis in the Changjiang river Dongting Lake waters, classical detection means
It is to carry out faecal egg detection（Also known as Kato-Katz methods）.However, traditional excrement extract worm's ovum method relate generally to homogenate,
The steps such as filtration, cleaning and smear, not only cumbersome time-consuming, extraction efficiency is low, and easily causes the false negative for detecting, with
And cause environmental pollution.
For the problems referred to above, having occurred correlation technique or device at present is used to improve the efficiency that worm's ovum is extracted in excrement,
These technologies or device are mostly put forth effort in the homogenate dilution operation of excrement, and with this extraction efficiency of worm's ovum is improved.However, whole
The steps such as excrement inspection flow process is further related to filter, cleaned, smear, and these steps still need independent instrument or equipment is aided in
Operation, can just complete whole excrement inspection flow process.Meanwhile, enrichment of the conventional method after worm's ovum is separated with excrement, purifying and smear
There is certain loss in operation, cause the inspection sensitivity of conventional method relatively low.In the face of above-mentioned deficiency, clinical examination is compeled to be essential
Want a kind of and easily realize automation, the purifying of high-sensitive parasite egg, the integrated apparatus for being enriched with and detecting.
The content of the invention
The technical problem to be solved is the drawbacks described above for overcoming prior art to exist, there is provided a kind of parasite worm
Worm's ovum is enriched with finder.
Second object of the present invention is to provide the method for carrying out parasite egg enrichment, observation using described device.
The purpose of the present invention is achieved by the following technical programs：
A kind of enrichment finder of parasite egg, including thin film chip and the slide unit above thin film chip are described thin
Transparent membrane of the membrane DNA chip by substrate and edge seal in substrate is constituted, and liquid storage cavity is formed between transparent membrane and substrate,
It is provided with the slide unit laterally, and the guide pillar being parallel to each other, equipped with the slide block that can be slided along guide pillar on guide pillar, the slide block
Top is provided with handle, and lower section is provided with slide plate, and the slide plate bottom is less than liquid storage cavity peak with substrate with the distance of substrate
Distance, the thin film chip is provided with the adjustable leg of height.
The operation principle of device of the present invention：Sample solution to be checked and magnetic particle solution are added to into liquid storage cavity
It is interior, while slip slide plate causes the solution in liquid storage cylinder body to be sufficiently mixed, magnet is placed in into one end of thin film chip, now magnetic
The compound of particle and worm's ovum is specifically bound, and heightens the height of leg, slowly moves slide block so that slide plate is rested on just
Isolate the position of magnetic-particle and the compound of parasite egg well；Finally whole worm's ovum finder is placed on microscope and is seen
Examine.
Because containing iron phosphorus compound in the shell of parasite egg, but this compound does not have paramagnetism, it is impossible to direct quilt
External magnetic is controlled, but can be attracted each other with magnetic-particle and combined to form compound, such that it is able to be indirectly controlled parasite worm
The enrichment procedure of ovum.Iron phosphorus compound is rich in especially for shells such as japonice ovums, its concentration effect is more preferably.
Sample to be checked and magnetic particle solution are added in thin film chip for convenience, it is preferable that set in the substrate
There is through hole, plug is housed on through hole, it is highly preferred that the plug is elastic plug.
In order to be more convenient observation, it is preferable that the substrate is substrate of glass；The thin film chip is by colourless, transparent material
Make.
Operate for convenience, the handle is protruded from outside slide unit；The slide unit both sides are additionally provided with supporting leg.
The present invention also provides a kind of method for carrying out worm's ovum observation using the parasite egg finder, including following
Step：
S1. sample solution to be checked is added in thin film chip, while adding magnetic particle solution, it is ensured that sample solution to be checked
2/3rds of liquid storage cavity volume is less than with the synthesis of the volume of magnetic particle solution；
S2. slide block is moved to by the side of slide unit one by handle, slidably reciprocate handle so that the solution in thin film chip is abundant
Mix；
S3. magnet is placed under a side of thin film chip, while sliding handle, waits magnetic-particle with parasite egg
Compound is enriched near the region of magnet；
S4. the height of leg is heightened, slide block is slowly moved so that slide plate rests on isolation magnetic-particle just and parasite worm
The position of the compound of ovum；
S5. whole worm's ovum finder is placed on microscope and is observed.
Compared with prior art, the invention has the advantages that：
The invention provides the enrichment finder and method of a kind of parasite egg, including thin film chip and positioned at thin film chip
The slide unit of top, transparent membrane of the thin film chip by substrate and edge seal in substrate constitute, transparent membrane and substrate
Between form liquid storage cavity, be provided with the slide unit laterally, and the guide pillar being parallel to each other, equipped with can slide along guide pillar on guide pillar
Slide block, be provided with handle above the slide block, lower section is provided with slide plate, and the slide plate bottom is less than liquid storage cylinder with the distance of substrate
The distance of body peak and substrate, the thin film chip is provided with the adjustable leg of height；By moving left and right for slide unit inner slide
Mixing operation is carried out to sample to be tested, the specific binding of magnetic bead and parasitic ovum is then passed through, and under the influence of a magnetic field, it is real
Existing worm's ovum enriching and purifying on chip, conveniently carries out follow-up microscopic examination.The present invention is applied to the row such as excrement, urine simultaneously
The parasitic ovum enrichment of thing is let out, especially schistosome ovum, because schistosome ovum shell is rich in iron, is combined more firm with magnetic-particle.
Therefore, the present invention not only realizes extraction of the parasitic ovum in complex samples, and is enriched with the shape that result can be directly used for clinic
State inspections and examinations.
Description of the drawings
Fig. 1 is the dimensional structure diagram that finder is enriched with described in embodiment 1（Perspective）；
Fig. 2 is the side sectional view that finder is enriched with described in embodiment 1；
Fig. 3 is to carry out sample to be checked using enrichment finder described in embodiment 1 to mix operation chart.
Fig. 4 is that finder carries out sample to be checked and magnetic particle solution mixes operation and shows using being enriched with described in embodiment 1
It is intended to.
Fig. 5 is the operation chart that magnetic enrichment is carried out using enrichment finder described in embodiment 1.
Description of reference numerals：1- slide units；2- handles；3- guide pillars；4- thin film chips；5- transparent membranes；6- plugs；7- is slided
Piece；8- slide blocks；9- magnet.
Specific embodiment
Patent of the present invention is further described with reference to specification drawings and specific embodiments.Wherein, accompanying drawing
Being for illustration only property illustrates that expression is only schematic diagram, rather than pictorial diagram, it is impossible to be interpreted as the restriction to this patent；In order to more
Illustrate the embodiment of patent of the present invention well, accompanying drawing some parts have omission, zoom in or out, and do not represent actual product
Size；It will be understood by those skilled in the art that some known features and its possible omission of explanation will be understood by accompanying drawing.
Embodiment 1
A kind of finder of parasite egg, including thin film chip 4 and the slide unit 1 positioned at the top of thin film chip 4, the film
Transparent membrane 5 of the chip 4 by substrate and edge seal in substrate is constituted, and liquid storage cavity is formed between transparent membrane 5 and substrate,
It is provided with the slide unit 1 laterally, and the guide pillar 3 being parallel to each other, it is described equipped with the slide block 8 that can be slided along guide pillar 3 on guide pillar 3
The top of slide block 8 is provided with handle 2, and lower section is provided with slide plate 7, and the bottom of the slide plate 7 is less than liquid storage cavity highest with the distance of substrate
The distance with substrate is put, the thin film chip 4 is provided with the adjustable leg of height.
The operation principle of device of the present invention：Sample solution to be checked and magnetic particle solution are added to into liquid storage cavity
It is interior, while slip slide plate 7 causes the solution in liquid storage cylinder body to be sufficiently mixed, magnet 9 is placed in into one end of thin film chip, now magnetic
Property particle and the compound of worm's ovum specifically bound, heighten the height of leg, slow mobile slide block 8 so that slide plate 7 is stopped
In the position of the compound of isolation magnetic-particle and parasite egg just；Finally whole worm's ovum finder is placed in into microscope
Upper observation.
Sample to be checked and magnetic particle solution are added in thin film chip 4 for convenience, in the present embodiment, the substrate
Through hole is provided with, plug 6 is housed on through hole, the plug is elastic plug.
In order to be more convenient observation, in the present embodiment, the substrate is substrate of glass；The thin film chip 4 is transparent by colourless
Material is made.
Operate for convenience, the handle 2 is protruded from outside slide unit 1；The both sides of the slide unit 1 are additionally provided with supporting leg.
Embodiment 2
The present invention a kind of utilization embodiment 1 is also provided described in the parasite egg finder method that carries out worm's ovum observation, including
Following steps：
S1. sample solution to be checked is added in thin film chip 4, while adding magnetic particle solution, it is ensured that sample solution to be checked
2/3rds of liquid storage cavity volume is less than with the synthesis of the volume of magnetic particle solution；
S2. slide block 8 is moved to by the side of slide unit 1 one by handle 2, slidably reciprocate handle 2 so that the solution in thin film chip 4
Fully mix；
S3. magnet 9 is placed under a side of thin film chip 4, while sliding handle 2, waits magnetic-particle and parasite egg
Compound be enriched in region near magnet 9；
S4. the height of leg is heightened, slow mobile slide block 8 so that slide plate 7 rests on isolation magnetic-particle and parasite just
The position of the compound of worm's ovum；
S5. whole worm's ovum finder is placed on microscope and is observed.
Claims (6)
1. the enrichment finder of a kind of parasite egg, it is characterised in that including thin film chip（4）With positioned at thin film chip
（4）The slide unit of top（1）, the thin film chip（4）By the transparent membrane of substrate and edge seal in substrate（5）Composition, thoroughly
Bright film（5）Liquid storage cavity, the slide unit are formed between substrate（1）Inside it is provided with laterally, and the guide pillar being parallel to each other（3）, lead
Post（3）Upper being equipped with can be along guide pillar（3）The slide block of slip（8）, the slide block（8）Top be provided with handle（2）, lower section is provided with cunning
Piece（7）, the slide plate（7）Bottom is less than the distance of liquid storage cavity peak and substrate, the thin film chip with the distance of substrate
（4）It is provided with the adjustable leg of height.
2. the enrichment finder of parasite egg according to claim 1, it is characterised in that the substrate is provided with logical
Hole, is equipped with plug on through hole（6）.
3. the enrichment finder of parasite egg according to claim 1, it is characterised in that the thin film chip is by nothing
Color, transparent material is made.
4. the enrichment finder of parasite egg according to claim 1, it is characterised in that the handle protrudes from cunning
Outside platform.
5. the enrichment finder of parasite egg according to claim 1, it is characterised in that the slide unit both sides also set
There is supporting leg.
6. the method that parasite egg enrichment finder described in a kind of any one of utilization claim 1 to 5 carries out worm's ovum observation,
Characterized in that, comprising the following steps：
S1. sample solution to be checked is added to into thin film chip（4）In, while adding magnetic particle solution, it is ensured that sample to be checked is molten
The synthesis of the volume of liquid and magnetic particle solution is less than 2/3rds of liquid storage cavity volume；
S2. handle is passed through（2）By slide block（8）Move to slide unit（1）One side, slidably reciprocate handle（2）So that thin film chip
（4）Interior solution is fully mixed；
S3. by magnet（9）It is placed in thin film chip（4）A side under, while sliding handle（2）, magnetic-particle is waited with parasitism
The compound of worm worm's ovum is enriched near magnet（9）Region；
S4. the height of leg is heightened, slide block is slowly moved（8）So that slide plate（7）Rest on and isolate magnetic-particle and post just
The position of the compound of infested worm's ovum；
S5. whole worm's ovum finder is placed on microscope and is observed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611209149.6A CN106645154A (en) | 2016-12-23 | 2016-12-23 | Enrichment and observation apparatus and method for parasite eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611209149.6A CN106645154A (en) | 2016-12-23 | 2016-12-23 | Enrichment and observation apparatus and method for parasite eggs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106645154A true CN106645154A (en) | 2017-05-10 |
Family
ID=58828381
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611209149.6A Pending CN106645154A (en) | 2016-12-23 | 2016-12-23 | Enrichment and observation apparatus and method for parasite eggs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106645154A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107271441A (en) * | 2017-07-07 | 2017-10-20 | 扬州大学 | A kind of method of utilization dark field microscope direct visual perception Cryptosporidium |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4494479A (en) * | 1982-10-14 | 1985-01-22 | Coulter Electronics, Inc. | Slide preparation apparatus |
| DE3936093A1 (en) * | 1988-11-04 | 1990-05-31 | Schwerin Bezirksnervenklinik | Cell concn. for microscopical investigation - by applying slide and retarding motion of cells by magnetic field with absorbent paper concentrating cells by extracting liq. |
| US20150056695A1 (en) * | 2013-08-26 | 2015-02-26 | Rarecyte, Inc. | Apparatus for spreading a fluid across a substrate and method of using the same |
| CN104697841A (en) * | 2015-03-30 | 2015-06-10 | 北京热景生物技术有限公司 | Magnetic particle separating and transferring device and method and application thereof |
| WO2016057374A1 (en) * | 2014-10-07 | 2016-04-14 | 3M Innovative Properties Company | Method of detecting an analyte using chromatographic enrichment |
| CN206339420U (en) * | 2016-12-23 | 2017-07-18 | 中山大学 | A kind of enrichment observation device of parasite egg |
-
2016
- 2016-12-23 CN CN201611209149.6A patent/CN106645154A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4494479A (en) * | 1982-10-14 | 1985-01-22 | Coulter Electronics, Inc. | Slide preparation apparatus |
| DE3936093A1 (en) * | 1988-11-04 | 1990-05-31 | Schwerin Bezirksnervenklinik | Cell concn. for microscopical investigation - by applying slide and retarding motion of cells by magnetic field with absorbent paper concentrating cells by extracting liq. |
| US20150056695A1 (en) * | 2013-08-26 | 2015-02-26 | Rarecyte, Inc. | Apparatus for spreading a fluid across a substrate and method of using the same |
| WO2016057374A1 (en) * | 2014-10-07 | 2016-04-14 | 3M Innovative Properties Company | Method of detecting an analyte using chromatographic enrichment |
| CN104697841A (en) * | 2015-03-30 | 2015-06-10 | 北京热景生物技术有限公司 | Magnetic particle separating and transferring device and method and application thereof |
| CN206339420U (en) * | 2016-12-23 | 2017-07-18 | 中山大学 | A kind of enrichment observation device of parasite egg |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107271441A (en) * | 2017-07-07 | 2017-10-20 | 扬州大学 | A kind of method of utilization dark field microscope direct visual perception Cryptosporidium |
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Application publication date: 20170510 |