CN106645062A - Method for laten fingerprint fluorescence development by applying aptamer and lysozyme for specific recognition and formation of G-quadruplex - Google Patents
Method for laten fingerprint fluorescence development by applying aptamer and lysozyme for specific recognition and formation of G-quadruplex Download PDFInfo
- Publication number
- CN106645062A CN106645062A CN201611082336.2A CN201611082336A CN106645062A CN 106645062 A CN106645062 A CN 106645062A CN 201611082336 A CN201611082336 A CN 201611082336A CN 106645062 A CN106645062 A CN 106645062A
- Authority
- CN
- China
- Prior art keywords
- fingerprint
- fluorescence
- aptamer
- specific recognition
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
Abstract
The invention relates to the technical field of laten fingerprint development, and in particular relates to a method for detecting laten fingerprints by fluorescence by applying aptamer and lysozyme recognition for formation of G-quadruplex cottage fluorescent molecules. The method comprises the following steps: (1) pressing a fingerprint: obtaining a latent perspiration fingerprint on the surface of a sample; (2) preparing a fluorescent development solvent: dissolving DNA into a Tris-HCl buffer solvent containing NaCl, MgCl2 and KCl, then adding an NMM solvent and completely mixing; (3) developing the laten fingerprint by fluorescence: dropwise applying the solvent obtained in the step (2) onto the surface of the laten fingerprint sample of the step (1) for reaction, quickly washing the dried sample by deionized water, and radiating under a ultraviolet source at a dark environment, so as to obtain a fingerprint pattern with clear lines, and obtaining an image of the fingerprint thorough a camera. The method provided by the invention has the advantages that the operation is simple, the cost is low, high efficiency and quickness are realized, the image is clear and easy to store and the like, and has larger application prospect in the field of criminal investigation.
Description
Technical field
The present invention relates to latent fingerprint appearing technique field, specifically a kind of to know with lysozyme with aptamer
Not, the method that the serobila cuttage fluorescence molecules of G tetra- carry out the latent fingerprint of fluoroscopic examination is formed.
Background technology
Fingerprint, because many factors such as inhereditary material, skin texture, pathology and trauma affect determine fingerprint ridge
With specificity, diversity, stability, can reach and personnel identity is carried out by the analysis to fingerprint ridge, identification, identification
Differentiate and assert.Fingerprint detection is one of relatively reliable method of person identification, and public security department can demonstrate,prove by fingerprint identification
Real crime and some styles of offence, have Great significance in related criminal investigation work.Generally fingerprint after stamp,
The material for staying in object surface is less, about 0.11mg, wherein 99% moisture can evaporate rapidly, in the material of residue about l%,
About 50% is the inorganic matters such as sodium chloride, potassium chloride, and they are not almost worth to fingerprint manifestation；Only remaining grease, amino
The purpose of fingerprint manifestation is can be only achieved after the organic principles such as acid and fluorescent material effect, therefore is found and fingerprint organic residue work
It is an important process for improving fingerprint manifestation rate and definition with the high reagent of sensitivity.According to the difference for manifesting principle, dive
Can be divided into following three major types in fingerprint manifestation method：Physisorphtion, chemical appearance method, optics appearance method.Physisorphtion
Because adsorbent and the combination being revealed between material are relatively unstable, the streakline for manifesting easily is destroyed, and easily forms some right
Human body has the dust of harm.Common chemical appearance method has silver nitrate method, ninhydrin method, " 502 " glue method, DFO methods etc..But by
To manifest label to react with some inorganic substances in finger mark in its principle, and such inorganic substances not finger mark is peculiar
And it is unstable, so its fingerprint for showing is difficult the reaction fingerprint unique characteristics of objective reality.Optics appearance method is to fingerprint
Strengthen presentation capability limited, it is impossible to independently solve the problem run in all practices, and in many cases, optics manifests required
Apparatus expensive, it is impossible to popularize.Application of micron to developing latent finger printss field has also obtained desk study, and various nano materials are
Jing is successfully applied in developing latent finger printss field, but the nanometer technology for manifesting latent fingerprint also has preparation process complexity, takes
The problems such as using high, it is impossible to substantial amounts of to be applied in social practice, therefore also need to explore new method for the aobvious of fingerprint of diving
It is existing.
The content of the invention
It is an object of the invention to provide a kind of use aptamer with lysozyme specific recognition and form the serobilas of G tetra-
Row fluorescence manifests the method for latent fingerprint, the method have it is simple to operate, it is with low cost, efficiently quick, imaging clearly it is easy to maintain etc.
Advantage.
A kind of utilization aptamer and lysozyme specific recognition simultaneously form the serobilas of G tetra- and carry out fluorescence and manifest latent fingerprint
Method, comprises the following steps：
1. fingerprint is restrained：Invisible fingerprints of sweat is obtained in sample surfaces；
2. fluorescence developing solution is prepared：DNA is dissolved in comprising NaCl, MgCl2With the Tris-HCl cushioning liquid of KCl
In, it is subsequently adding NMM solution and is sufficiently mixed；
3. fluorescence manifests latent fingerprint：Latent fingerprint sample surface reaction of the solution drop coating that 2. step is obtained to step 1.
Afterwards, the dried sample of deionized water Rapid Cleaning, is under ultraviolet source in dark and irradiates, you can obtain lines clearly
Fingerprint pattern, by camera the fingerprint image is obtained.
1. middle sample is glass, tinfoil paper, plastics or sheet metal to the step.
The DNA sequence dna is AP1：5’-TgggTATgTCTTTATCAgggCTAAAgAg- 3 ' and AP2：5’-TgCAgAgTTACTTAgTTTgACATgggTAgggCggg-3’.Wherein, two segment DNA sequences of horizontal line part are altogether bacteriolyzes
The aptamers sequence of enzyme, and two parts sequence of italicized item is altogether the sequence of the serobilas of G tetra-.
The mol ratio of DNA and NMM is 1 in the fluorescence developing solution:5～1:8.
The mol ratio of DNA and NMM is 1 in the fluorescence developing solution:5～1:8, preferably 1:6.
The DNA concentration is 10～50 μM, preferably 20～40 μM, more preferably 30 μM；The concentration of the NMM is 50～300 μ
M；The concentration of the Tris-HCl cushioning liquid be 30～50mM, preferred 40mM, add NaCl concentration be 150～250mM,
MgCl2It is 40～60mM for 5～15mM, KCl, preferred NaCl is 200mM, MgCl2It is 50mM for 10mM, KCl.
3. the middle reaction time is 30～60min to the step.
The step 3. middle ultraviolet source wavelength be 300～400nm.
The technological principle of the present invention：Aptamer is artificial screening to the object (bacteriolyze in line sweat residue
Enzyme) there is the affinity and selective specific dna sequence of height.Therefore, the lysozyme in fingerprint ridge residue can be suitable with it
Ligand specificity combines, and makes two sections of aptamers DNA (i.e. AP1, AP2) of fracture further, two sections of rich G sequences of aptamers end
Also further therewith, in the presence of potassium ion, form the stranded structures of G tetra-, so as to cuttage NMM, send fluorescence signal, and free
NMM does not have fluorescence, therefore, only can just there is fluorescence in the position for having fingerprint residues thing, can be taken turns under the exciting of uviol lamp
It is wide substantially, lines clearly fingerprint fluorescent image.
Beneficial effects of the present invention：
(1) DNA aptamers used in the present invention need not be modified, with low cost.
(2) present invention can manifest fingerprint in the sample surfaces of unlike material, and range of application is wide.
(3) present invention can be shown latent fingerprint by fluorescence at short notice, it is not necessary to expend too many manpower
The resources such as material resources, can quickly recognize personal information.
(4) present invention to latent fingerprint have preferably manifest effect, have the advantages that it is simple, quick, easy to use,
Criminal investigation field has larger application prospect.
Description of the drawings
Fig. 1 (a), Fig. 1 (b), Fig. 1 (c) are respectively embodiment 1 10 μm of ol/L of different DNA concentrations, 30 μ on masking foil
The developing latent finger printss design sketch of mol/L, 50 μm of ol/L.
Fig. 2 is developing latent finger printss figure of the embodiment 2 on glass.
Fig. 3 is developing latent finger printss figure of the embodiment 3 on preservative film.
Fig. 4 is developing latent finger printss figure of the embodiment 4 on plastics.
Fig. 5 is developing latent finger printss figure of the embodiment 5 on coin.
Specific embodiment
With reference to embodiment, the present invention is described further.
Embodiment 1：The developing latent finger printss experiment that the DNA of variable concentrations is used on masking foil
1. fingerprint is restrained：To be hand-washed totally with hand cleanser, and PE gloves be put on after drying and seals sweat 10 minutes, with finger with appropriateness
Strength restrain and obtain invisible fingerprints of sweat in masking foil surface；
2. fluorescence developing solution is prepared：DNA is dissolved in comprising 200mM NaCl, 10mM MgCl2With 50mM KCl's
In Tris-HCl (40mM) cushioning liquid, 10 μM are made into, the solution of 30 μM and 50 μM concentration is then respectively adding 60 μM, 180 μM
NMM with 300 μM, obtains fluorescence developing solution.
3. fluorescence manifests latent fingerprint：Latent fingerprint sample surface of the solution drop coating that 2. step is obtained to step 1., reaction
After 40min, the dried sample of deionized water Rapid Cleaning is under ultraviolet source in dark and irradiates, and is obtained by camera
The fluoroscopic image of the fingerprint, is shown in Fig. 1 (a), Fig. 1 (b), Fig. 1 (c).
As can be seen from the figure when the concentration of DNA adaptations liquid solution is different, also different, the concentration mistake that manifests effect of latent fingerprint
It is low or it is too high be all unfavorable for manifesting for latent fingerprint, this is because concentration is too low, fluorescence is too weak, developing latent finger printss not substantially, and
Concentration increases, Fluorescence Increasing, but concentration it is excessive when, non-specific adsorption strengthens, and causes background fluorescence also to strengthen, therefore concentration
When too high, the contrast of fingerprint lines declines on the contrary, that is, the effect that manifests of fingerprint of diving declines.So, manifest the best DNA of effect
Concentration is 30 μM.
Embodiment 2：Developing latent finger printss experiment on glass
1. fingerprint is restrained：To be hand-washed totally with hand cleanser, and PE gloves be put on after drying and seals sweat 10 minutes, with finger with appropriateness
Strength restrain and obtain invisible fingerprints of sweat in glass surface；
2. fluorescence developing solution is prepared：DNA is dissolved in comprising 200mM NaCl, 10mM MgCl2With 50mM KCl's
In Tris-HCl (40mM) cushioning liquid, the solution of 30 μM of concentration is made into, is then respectively adding 150 μM of NMM, obtained fluorescence and show
Color solution.
3. fluorescence manifests latent fingerprint：Latent fingerprint sample surface of the solution drop coating that 2. step is obtained to step 1., reaction
After 50min, the dried sample of deionized water Rapid Cleaning is under ultraviolet source in dark and irradiates, and is obtained by camera
The fluoroscopic image of the fingerprint, is shown in Fig. 2.
As can be seen from Figure 2 the method can also show the latent fingerprint restrained on glass, and reduce the ratio of NMM and contain
The lighter of fingerprint image after amount, brightness is dimmed, but remains to be clearly visible that the lines of latent fingerprint.
Embodiment 3：Developing latent finger printss experiment on preservative film
1. fingerprint is restrained：To be hand-washed totally with hand cleanser, and PE gloves be put on after drying and seals sweat 12 minutes, with finger with appropriateness
Strength restrain and obtain invisible fingerprints of sweat in preservative film surface；
2. fluorescence developing solution is prepared：DNA is dissolved in comprising 200mM NaCl, 10mM MgCl2With 50mM KCl's
In Tris-HCl (40mM) cushioning liquid, the solution of 30 μM of concentration is made into, is then respectively adding 170 μM of NMM, obtained fluorescence and show
Color solution.
3. fluorescence manifests latent fingerprint：Latent fingerprint sample surface of the solution drop coating that 2. step is obtained to step 1., reaction
After 30min, the dried sample of deionized water Rapid Cleaning is under ultraviolet source in dark and irradiates, and is obtained by camera
The fluoroscopic image of the fingerprint, is shown in Fig. 3.
As can be seen from Figure 3 the method can show the fingerprint restrained on preservative film surface, can clearly show
The lines of latent fingerprint.
Embodiment 4：Developing latent finger printss experiment on plastics
1. fingerprint is restrained：To be hand-washed totally with hand cleanser, and PE gloves be put on after drying and seals sweat 8 minutes, with finger with appropriateness
Strength restrain and obtain invisible fingerprints of sweat in frosting；
2. fluorescence developing solution is prepared：DNA is dissolved in comprising 200mM NaCl, 10mM MgCl2With 50mM KCl's
In Tris-HCl (40mM) cushioning liquid, the solution of 30 μM of concentration is made into, is then respectively adding 200 μM of NMM, obtained fluorescence and show
Color solution.
3. fluorescence manifests latent fingerprint：Latent fingerprint sample surface of the solution drop coating that 2. step is obtained to step 1., reaction
After 45min, the dried sample of deionized water Rapid Cleaning is under ultraviolet source in dark and irradiates, and is obtained by camera
The fluoroscopic image of the fingerprint, is shown in Fig. 4.
Because plastics are hydrophobic surfaces, DNA fluorescence developing solution to sprawl effect poor, cause to manifest effect
It is less desirable, but from fig. 4, it can be seen that the latent fingerprint on plastics can also be displayed with the method.
Embodiment 5：Developing latent finger printss experiment on coin
1. fingerprint is restrained：To be hand-washed totally with hand cleanser, and PE gloves be put on after drying and seals sweat 10 minutes, with finger with appropriateness
Strength restrain and obtain invisible fingerprints of sweat in unitary coin surface；
2. fluorescence developing solution is prepared：DNA is dissolved in comprising 200mM NaCl, 10mM MgCl2With 50mM KCl's
In Tris-HCl (40mM) cushioning liquid, the solution of 40 μM of concentration is made into, is then respectively adding 240 μM of NMM, obtained fluorescence and show
Color solution.
3. fluorescence manifests latent fingerprint：Latent fingerprint sample surface of the solution drop coating that 2. step is obtained to step 1., reaction
After 35min, the dried sample of deionized water Rapid Cleaning is under ultraviolet source in dark and irradiates, and is obtained by camera
The fluoroscopic image of the fingerprint, is shown in Fig. 5.
Although coin uneven surface, as can be seen from Figure 5 the method can show the fingerprint restrained on coin,
This explanation the method can be not only used for the developing latent finger printss of flat material surface, it is also possible to for nonplanar material surface
Developing latent finger printss.
Claims (8)
1. a kind of utilization aptamer and lysozyme specific recognition and form the serobilas of G tetra- and carry out the side that fluorescence manifests latent fingerprint
Method, it is characterised in that comprise the following steps：
1. fingerprint is restrained：Invisible fingerprints of sweat is obtained in sample surfaces；
2. fluorescence developing solution is prepared：DNA is dissolved in comprising NaCl, MgCl2In the Tris-HCl cushioning liquid of KCl, then
NMM solution is added to be sufficiently mixed；
3. fluorescence manifests latent fingerprint：After latent fingerprint sample surface of the solution drop coating that 2. step is obtained to step 1. is reacted, use
The dried sample of deionized water Rapid Cleaning, is under ultraviolet source in dark and irradiates, you can obtain lines clearly fingerprint
Pattern, by camera the fingerprint image is obtained.
2. one kind according to claim 1 uses aptamer with lysozyme specific recognition and forms the serobilas of G tetra-
Row fluorescence manifests the method for latent fingerprint, it is characterised in that 1. middle sample is glass, tinfoil paper, plastics or sheet metal to the step.
3. one kind according to claim 1 uses aptamer with lysozyme specific recognition and forms the serobilas of G tetra-
Row fluorescence manifests the method for latent fingerprint, it is characterised in that the DNA sequence dna is AP1：5’-
TgggTATgTCTTTATCAgggCTAAAgAg- 3 ' and AP2：5’-TgCAgAgTTACTTAgTTTgACA TgggTAgggCggg-
3’。
4. the one kind according to claim 1-3 any one is with aptamer is with lysozyme specific recognition and is formed
The serobilas of G tetra- carry out the method that fluorescence manifests latent fingerprint, it is characterised in that the mol ratio of DNA and NMM in the fluorescence developing solution
For 1:5～1:8.
5. the one kind according to claim 1-3 any one is with aptamer is with lysozyme specific recognition and is formed
The serobilas of G tetra- carry out the method that fluorescence manifests latent fingerprint, it is characterised in that the DNA concentration is 10～50 μM, and the NMM's is dense
To spend for 50～300 μM, the concentration of the Tris-HCl cushioning liquid is 30～50mM, the concentration for adding NaCl is 150～
250mM、MgCl2It is 40～60mM for 5～15mM, KCl.
6. one kind according to claim 5 uses aptamer with lysozyme specific recognition and forms the serobilas of G tetra-
Row fluorescence manifests the method for latent fingerprint, it is characterised in that the mol ratio of DNA and NMM is 1 in the fluorescence developing solution:6, institute
DNA concentration is stated for 30 μM, the concentration of the Tris-HCl cushioning liquid is 40mM.
7. one kind according to claim 1 uses aptamer with lysozyme specific recognition and forms the serobilas of G tetra-
Row fluorescence manifests the method for latent fingerprint, it is characterised in that 3. the middle reaction time is 30～60min to the step.
8. one kind according to claim 1 uses aptamer with lysozyme specific recognition and forms the serobilas of G tetra-
Row fluorescence manifests the method for latent fingerprint, it is characterised in that the step 3. middle ultraviolet source wavelength be 300～400nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611082336.2A CN106645062B (en) | 2016-11-30 | 2016-11-30 | With aptamer and lysozyme specific recognition and forms tetra- serobila of G and carry out the method that fluorescence shows latent fingerprint |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611082336.2A CN106645062B (en) | 2016-11-30 | 2016-11-30 | With aptamer and lysozyme specific recognition and forms tetra- serobila of G and carry out the method that fluorescence shows latent fingerprint |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106645062A true CN106645062A (en) | 2017-05-10 |
| CN106645062B CN106645062B (en) | 2019-03-19 |
Family
ID=58814413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611082336.2A Expired - Fee Related CN106645062B (en) | 2016-11-30 | 2016-11-30 | With aptamer and lysozyme specific recognition and forms tetra- serobila of G and carry out the method that fluorescence shows latent fingerprint |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106645062B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110464361A (en) * | 2019-08-06 | 2019-11-19 | 中南民族大学 | A kind of method that enzymatic reaction system shows for latent fingerprint |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130078622A1 (en) * | 2011-09-26 | 2013-03-28 | The Regents Of The University Of California | Electronic device for monitoring single molecule dynamics |
| CN103630517A (en) * | 2013-11-15 | 2014-03-12 | 南京邮电大学 | Thrombin detection method based on splitter adapter and water-soluble conjugated polymer |
| CN103992788A (en) * | 2014-05-13 | 2014-08-20 | 中国科学院长春应用化学研究所 | Coronene derivative probe and preparation method thereof, and protein detection method based on coronene derivative probe and aptamer |
-
2016
- 2016-11-30 CN CN201611082336.2A patent/CN106645062B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130078622A1 (en) * | 2011-09-26 | 2013-03-28 | The Regents Of The University Of California | Electronic device for monitoring single molecule dynamics |
| CN103630517A (en) * | 2013-11-15 | 2014-03-12 | 南京邮电大学 | Thrombin detection method based on splitter adapter and water-soluble conjugated polymer |
| CN103992788A (en) * | 2014-05-13 | 2014-08-20 | 中国科学院长春应用化学研究所 | Coronene derivative probe and preparation method thereof, and protein detection method based on coronene derivative probe and aptamer |
Non-Patent Citations (3)
| Title |
|---|
| 昌珍珍: "基于核酸适配体的非标记荧光检测方法研究", 《中国优秀硕士学位论文全文数据库》 * |
| 李名慧等: "富G寡核苷酸-N-甲基卟啉二丙酸荧光法测定铀", 《应用化工》 * |
| 李志豪等: "稀土上转换发光纳米探针用于潜指纹中***的检测", 《分析科学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110464361A (en) * | 2019-08-06 | 2019-11-19 | 中南民族大学 | A kind of method that enzymatic reaction system shows for latent fingerprint |
| CN110464361B (en) * | 2019-08-06 | 2022-04-05 | 中南民族大学 | Method for potential fingerprint display by enzymatic reaction system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106645062B (en) | 2019-03-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bumbrah et al. | Emerging latent fingerprint technologies: a review | |
| Jin et al. | A tetraphenylethene-based dye for latent fingerprint analysis | |
| Yang et al. | Studies on the development of latent fingerprints by the method of solid–medium ninhydrin | |
| Chuena et al. | Forensic light sources for detection of biological evidences in crime scene investigation: a review | |
| CN106974659A (en) | A kind of latent fingerprint detection method based on red fluorescence carbon point material | |
| Edwards | Probing history with Raman spectroscopy | |
| CN105907389A (en) | Preparation methods of noble-metal nanocluster development reagents and sweat fingerprint development method | |
| Malik et al. | Advances in conjugated polymers for visualization of latent fingerprints: a critical perspective | |
| CN103431867A (en) | Method for displaying latent fingerprints on fluorogold nano-cluster protected by protein | |
| Xu et al. | Non-destructive enhancement of latent fingerprints on stainless steel surfaces by electrochemiluminescence | |
| Barros et al. | Fluorescent benzazole dyes for bloodstain detection and bloody fingermark enhancement | |
| Cadd et al. | The non-contact detection and identification of blood stained fingerprints using visible wavelength hyperspectral imaging: Part II effectiveness on a range of substrates | |
| CN109557066B (en) | Preparation method of copper nano-cluster sweat latent fingerprint developing reagent and sweat latent fingerprint developing method | |
| CN103784149B (en) | A kind of many metal deposition technique manifest the method for laten fingerprints | |
| CN106645062B (en) | With aptamer and lysozyme specific recognition and forms tetra- serobila of G and carry out the method that fluorescence shows latent fingerprint | |
| Yang et al. | Handy, rapid and multiplex detection of tumor markers based on encoded silica–hydrogel hybrid beads array chip | |
| CN108315011A (en) | Fingerprint imaging composition and its application | |
| CN104337522A (en) | Method for displaying latent fingerprints by adopting fluorescent dye-modified nano particles | |
| Haan | Physics and fingerprints | |
| Hong et al. | Development of dry-origin latent footwear impression on non-porous and semi-porous surfaces using a 5-methylthioninhydrin and L-alanine complex | |
| Zarate et al. | A fluorogenic method for lifting, enhancing, and preserving bloody impression evidence | |
| Fabiszak | Cyanoacrylate fuming and white powder suspension together in one sequence for nonporous surfaces | |
| Fan et al. | Strongly fluorescent conjugated polymer nanoparticles in aqueous colloidal solution for universal, efficient and effective development of sebaceous and blood fingerprints | |
| Van Der Spil | Evaluating the efficiency and sensitivity of one-step cyanoacrylate fuming methods in the detection and visualisation of latent fingerprints on the adhesive side of tape | |
| Kent et al. | Sequential treatment and enhancement |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190319 Termination date: 20191130 |