CN1066290A - Nutritive element yeast production technique - Google Patents
Nutritive element yeast production technique Download PDFInfo
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- CN1066290A CN1066290A CN 92104003 CN92104003A CN1066290A CN 1066290 A CN1066290 A CN 1066290A CN 92104003 CN92104003 CN 92104003 CN 92104003 A CN92104003 A CN 92104003A CN 1066290 A CN1066290 A CN 1066290A
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- yeast
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- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The present invention is a kind of nutritive element yeast production technique that is used to produce microbial product.It adopts and selects anti-zinc yeast strain or anti-iron yeast strain for use in the strain culturing process, in seeding tank or commodity jar inoculation culture process, in substratum, add zinc sulfate or ferrous sulfate respectively,, obtain Zinc-rich saccharomyces cerevisiae or ferrum-rich saccharomyces cerevisiae through corresponding technological process; Perhaps select rich ergosterol yeast strain for use, carry out the rich ergosterol yeast that makes moistening and the screening processing,, obtain vitamins D through uviolizing and cryodrying process in the strain culturing process
2The yeast finished product.The advantage that has the simple to operate and good process repeatability of production process with the present invention.
Description
The present invention relates to a kind of production technique of microbial product, particularly a kind of nutritive element yeast production technique.
The yeast that prior art is produced, the content of its zinc and iron is very low, can not satisfy special requirement; Vitamin D2 occurs with the crystalline form mostly simultaneously, and its production technique is miscellaneous, technological process length consuming time, and product is difficult to store.
The objective of the invention is to provide a kind of nutritive element yeast production technique, adopt this technology can produce Zinc-rich saccharomyces cerevisiae and ferrum-rich saccharomyces cerevisiae, can also produce the vitamin D2 yeast product of easy storage simultaneously, its operating process is simple, good process repeatability.
The object of the present invention is achieved like this: it adopts technological processs such as strain culturing, F bottle graft kind, Carlsberg's flask inoculation, the inoculation of pure culture jar, seeding tank inoculation, the inoculation of commodity jar, separating, washing, filter press, self-dissolving insulation, spraying drying and finished product packing.When producing Zinc-rich saccharomyces cerevisiae, what the strain culturing process adopted is the anti-zinc yeast strain of process screening, and seeding tank inoculation and commodity jar seeded process are added zinc sulfate respectively in substratum; Perhaps when producing ferrum-rich saccharomyces cerevisiae, what the strain culturing process adopted is the anti-iron yeast strain of process screening, and seeding tank inoculation and commodity jar seeded process are added ferrous sulfate respectively in substratum; Perhaps when producing the vitamin D2 yeast, what the strain culturing process adopted is the rich ergosterol yeast strain of process screening, carry out moistening as work in-process and the screening processing with the rich ergosterol yeast of making,, obtain vitamin D2 yeast finished product through uviolizing and cryodrying process.18 hours time in seeding tank inoculation culture process, was that the master makes substratum with molasses, and stream adds ammonium sulfate (NH simultaneously
4)
2SO
4, concentration is 0.1-3mol/m
3Zinc sulfate ZnSO
47H
2O, phosphorus source and trace element, 30 ℃ to 31 ℃ of culture temperature, incubation time 30 hours; In commodity jar inoculation culture process, be that main body is made substratum with molasses, stream adds ammonium sulfate (NH simultaneously
4)
2SO
4, concentration is 0.1-3mol/m
3Zinc sulfate ZnSO
47H
2O, phosphorus source and trace element; The fermented liquid of commodity jar is carried out separating, washing; Carrying out press filtration with plate-and-frame filter press handles; Self-dissolving in the self-dissolving jar is incubated 1 hour down in 95 ℃ of states, finishes the self-dissolving insulating process; Spray-dried, the water content of Zinc-rich saccharomyces cerevisiae is controlled at below 8%, last, the finished product packing process is finished in the packing instructions of pressing food or pharmaceutical raw material.
Embodiment two: when producing ferrum-rich saccharomyces cerevisiae, at first screening an anti-iron yeast strain, is that substratum carries out test tube slant cultivation, 30 ℃ of culture temperature, incubation time 48 hours with the malt agar; With the wort is that substratum carries out the cultivation of F bottle graft kind, 30 ℃ of culture temperature, incubation time 48 hours; With wort and molasses mixed solution is that substratum carries out the Carlsberg's flask inoculation culture, 30 ℃ of culture temperature, incubation time 48 hours; With molasses is that substratum carries out pure culture jar inoculation culture, incubation time 18 hours; In seeding tank inoculation culture process, be that the master makes substratum with molasses, stream adds ammonium sulfate (NH simultaneously
4)
2SO
4, concentration is 0.1-3mol/m
3Ferrous sulfate FeSO
47H
2O, phosphorus source and trace element, 30 ℃ to 31 ℃ of culture temperature, incubation time 30 hours; Connect wherein at the commodity jar, when producing the vitamin D2 yeast, rich ergosterol yeast water or alcohol carry out moistening processing, make moisture content or ethanol content reach 5% to 80%; Adopt 40 eye mesh screens to sieve processing; With the uviolizing of 280nm to 310nm 30 minutes to 90 minutes; Under 50 ℃ to 60 ℃ temperature, finish the cryodrying process.
The present invention has selected anti-iron yeast strain owing to selected anti-zinc yeast strain for use when producing Zinc-rich saccharomyces cerevisiae for use when producing ferrum-rich saccharomyces cerevisiae, thereby makes nutritive element yeast such as producing rich zinc and Fu Tie become possibility; Simultaneously because in seeding tank inoculation and commodity jar seeded process, interpolation zinc sulfate or ferrous sulfate in substratum respectively are so improved the content of zinc in the yeast or iron effectively; In addition owing to directly accept the method for uviolizing after adopting the moistening and screening of rich ergosterol, so simplified vitamin D2 zymic production process.The present invention also has simple to operate and advantage good process repeatability.
Specific embodiment of the present invention is provided by following embodiment.
Embodiment one: when producing Zinc-rich saccharomyces cerevisiae, at first screening an anti-zinc yeast strain, is that substratum carries out test tube slant cultivation, 30 ℃ of culture temperature, incubation time 48 hours with the malt agar; With the wort is that substratum carries out 30 ℃ of the cultivation of F bottle graft kind, culture temperature, incubation time 48 hours; With wort and molasses mixed solution is that substratum carries out the Carlsberg's flask inoculation culture, 30 ℃ of culture temperature, incubation time 48 hours; With molasses is that substratum carries out pure culture jar inoculation culture, cultivate in kind of the culturing process, and be that main body is made substratum with molasses, stream adds ammonium sulfate (NH simultaneously
4)
2SO
4, concentration is 0.1-3mol/m
3Ferrous sulfate FeSO
47H
2O, phosphorus source and trace element; The fermented liquid of commodity jar is carried out separating, washing, carry out press filtration with plate-and-frame filter press and handle; Self-dissolving in the self-dissolving jar is incubated 1 hour down in 95 ℃ of states, finishes the self-dissolving insulating process; Spray-dried, the water content of ferrum-rich saccharomyces cerevisiae is controlled at below 8%, last, the finished product packing process is finished in the packing instructions of pressing food or pharmaceutical raw material.
Embodiment three: when producing the vitamin D2 yeast, at first screening the yeast strain of a rich ergosterol, is that substratum carries out test tube slant cultivation, 30 ℃ of culture temperature, incubation time 48 hours with the malt agar; With the wort is that substratum carries out the cultivation of F bottle graft kind, 30 ℃ of culture temperature, incubation time 48 hours; With wort and molasses mixed solution is that substratum carries out the Carlsberg's flask inoculation culture, 30 ℃ of culture temperature, incubation time 48 hours; With molasses is that substratum carries out pure culture jar inoculation culture, incubation time 18 hours; In seeding tank inoculation culture process, be the main substratum of doing with molasses, increase and ventilate, add nitrogenous source less, increase the phosphorus source, 30 ℃ to 31 ℃ of culture temperature, incubation time 30 hours; In commodity jar inoculation culture process, be that main body is made substratum with molasses, increase and ventilate, add less nitrogenous source, increase phosphorus source; The fermented liquid of commodity jar is carried out separating, washing, carry out press filtration with plate-and-frame filter press and handle; Self-dissolving in the self-dissolving jar is incubated 1 hour down in 95 ℃ of states, finishes the self-dissolving insulating process; Spray-dried, rich ergosterol zymic water content is controlled at below 8%, get rich ergosterol yeast; With rich ergosterol yeast water or to spill essence evenly moistening, sieve processing with 40 eye mesh screens, make its moisture content or ethanol content reach 5% to 80%, be laid on yeast on the travelling belt equably again, the travelling belt top is provided with quartz burner, slow moving conveyor belt, it simultaneously is the uviolizing 30 minutes to 90 minutes of 280nm to 310nm with the wavelength, again yeast is placed and carry out cryodrying under 50 ℃ to 60 ℃ the state of temperature and handle, the vitamin D2 yeast that obtains by food or pharmaceutical packing requirement, is finished the finished product packing process.
Claims (2)
1, a kind of nutritive element yeast production technique, it comprises following technological process: strain culturing, F bottle graft kind, Carlsberg's flask inoculation, the inoculation of pure culture jar, seeding tank inoculation, the inoculation of commodity jar, separating, washing, filter press, self-dissolving insulation, spraying drying and finished product packing, it is characterized in that: when producing Zinc-rich saccharomyces cerevisiae, what the strain culturing process adopted is the anti-zinc yeast strain of process screening, and seeding tank inoculation and commodity jar seeded process are added zinc sulfate respectively in substratum; Perhaps when producing ferrum-rich saccharomyces cerevisiae, what the strain culturing process adopted is the anti-iron yeast strain of process screening, and seeding tank inoculation and commodity jar seeded process are added ferrous sulfate respectively in substratum; Perhaps when producing the vitamin D2 yeast, what the strain culturing process adopted is the rich ergosterol yeast strain of process screening, carry out moistening as work in-process and the screening processing with the rich ergosterol yeast of making,, obtain vitamin D2 yeast finished product through uviolizing and cryodrying process.
2, nutritive element yeast production technique according to claim 1 is characterized in that: when producing the vitamin D2 yeast, rich ergosterol yeast water or alcohol carry out moistening processing, and moisture content or ethanol content reach 5% to 80%; Adopt tool 40 eye mesh screens to sieve processing; With wavelength is the uviolizing 30 minutes to 90 minutes of 280nm to 310nm; Under 50 ℃ to 60 ℃ temperature, finish the cryodrying process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92104003 CN1066290A (en) | 1992-05-21 | 1992-05-21 | Nutritive element yeast production technique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92104003 CN1066290A (en) | 1992-05-21 | 1992-05-21 | Nutritive element yeast production technique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1066290A true CN1066290A (en) | 1992-11-18 |
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ID=4940560
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92104003 Pending CN1066290A (en) | 1992-05-21 | 1992-05-21 | Nutritive element yeast production technique |
Country Status (1)
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|---|---|
| CN (1) | CN1066290A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322109C (en) * | 2002-10-15 | 2007-06-20 | 湖北安琪酵母股份有限公司 | Drying production technique of fluidized bed by starch adsorption of dry oceanic rhodotorula |
| CN101575579B (en) * | 2008-05-06 | 2012-01-04 | 安琪酵母股份有限公司 | Ferrum-rich saccharomyces cerevisiae and production method thereof |
| CN105861480A (en) * | 2015-01-19 | 2016-08-17 | 安琪酵母股份有限公司 | Yeast enriching vitamin D2 and production technology thereof |
-
1992
- 1992-05-21 CN CN 92104003 patent/CN1066290A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322109C (en) * | 2002-10-15 | 2007-06-20 | 湖北安琪酵母股份有限公司 | Drying production technique of fluidized bed by starch adsorption of dry oceanic rhodotorula |
| CN101575579B (en) * | 2008-05-06 | 2012-01-04 | 安琪酵母股份有限公司 | Ferrum-rich saccharomyces cerevisiae and production method thereof |
| CN105861480A (en) * | 2015-01-19 | 2016-08-17 | 安琪酵母股份有限公司 | Yeast enriching vitamin D2 and production technology thereof |
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| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |