CN106623406B - A method of joint repairs waste water and acid soil - Google Patents
A method of joint repairs waste water and acid soil Download PDFInfo
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- CN106623406B CN106623406B CN201611206579.2A CN201611206579A CN106623406B CN 106623406 B CN106623406 B CN 106623406B CN 201611206579 A CN201611206579 A CN 201611206579A CN 106623406 B CN106623406 B CN 106623406B
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- 239000002351 wastewater Substances 0.000 title claims abstract description 48
- 239000002689 soil Substances 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 20
- 239000002253 acid Substances 0.000 title claims abstract description 18
- 230000008439 repair process Effects 0.000 title claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims abstract description 25
- 241000195493 Cryptophyta Species 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000002054 inoculum Substances 0.000 claims abstract description 14
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 10
- 239000002131 composite material Substances 0.000 claims abstract description 10
- 150000001875 compounds Chemical class 0.000 claims abstract description 9
- 238000003973 irrigation Methods 0.000 claims abstract description 5
- 230000002262 irrigation Effects 0.000 claims abstract description 5
- 238000002474 experimental method Methods 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 9
- 239000002609 medium Substances 0.000 claims description 7
- 239000006916 nutrient agar Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 241000131970 Rhodospirillaceae Species 0.000 claims description 2
- 238000005286 illumination Methods 0.000 claims 1
- 239000013049 sediment Substances 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 238000012545 processing Methods 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 2
- 235000011941 Tilia x europaea Nutrition 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 239000004571 lime Substances 0.000 description 2
- 239000011572 manganese Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000005067 remediation Methods 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241001122767 Theaceae Species 0.000 description 1
- AZDRQVAHHNSJOQ-UHFFFAOYSA-N alumane Chemical class [AlH3] AZDRQVAHHNSJOQ-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012958 reprocessing Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/30—Wastewater or sewage treatment systems using renewable energies
- Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Botany (AREA)
- Ecology (AREA)
- Soil Sciences (AREA)
- Processing Of Solid Wastes (AREA)
- Fertilizers (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The present invention provides a kind of methods that joint repairs waste water and acid soil, specifically comprise the following steps: (1) phycomycete culture: algae strain and compound bacteria are seeded in the container waste water to be processed for filling waste water to be processed, it is cultivated under solar irradiation and greenhouse experiment, until the pH of water reaches 10 or more in container；It is 0.05-0.07 that the inoculum concentration of the algae strain, which is initial OD 680,；The inoculum concentration of the compound bacteria is 10mL composite bacteria agent/1L waste water；(2) irrigation soils: acid soil is irrigated with the water that phycomycete culture is handled, the pH of soil is made to reach 6 or more.Waste water and acid soil can be repaired simultaneously by this method, and repairing quality is satisfactory.Compared with the method for individually repairing waste water and acid soil at present, this method has the advantages that put into that low, output is high, do not need chemicals, does not generate that sediment, sustainability are strong and quality is high.
Description
Technical field
The invention belongs to waste water and soil ecology to repair field, in particular to a kind of joint repairs the side of waste water and acid soil
Method.
Background technique
The cultivable land of global nearly half has that acidity limits crop-producing power, corrects soil acid for a long time
The main method of property is liming.But liming there are higher cost, slow effect and only to topsoil effectively etc. ask
Topic.There is presently no the methods and techniques of satisfactory correction soil acidity.On the other hand, the production of global range internal cause and life
The raw a large amount of waste water of life birth, the method for handling waste water at present needs expensive equipment, material and the energy, and produces during processing
Raw a large amount of toxic sediments, cause secondary pollution, therefore wastewater treatment faces technical challenge.For example, China has every year
6000000000 tons of Pig raising wastewater is not appropriately processed and discharges.As can joint reparation is carried out to waste water and acid soil, it can be to greatest extent
Reduction investment, increase output, avoid generating processing sediment, and repairing quality can be improved.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide the side of a kind of joint reparation waste water and acid soil
Method.
The purpose of the present invention is achieved through the following technical solutions: a kind of joint repairs the side of waste water and acid soil
Method specifically comprises the following steps:
(1) phycomycete culture: algae strain and compound bacteria are seeded in the container waste water to be processed for filling waste water to be processed, In
It is cultivated under solar irradiation and greenhouse experiment, until the pH of water reaches 10 or more in container；The inoculum concentration of the algae strain is first
Beginning OD680 is 0.05-0.07；The inoculum concentration of the compound bacteria is 10mL composite bacteria agent/1L waste water；
(2) irrigation soils: acid soil is irrigated with the water that phycomycete culture is handled, the pH of soil is made to reach 6 or more.
Further, the preparation process of the composite bacteria agent is as follows: waste water being taken to be added separately to Van Niel Purple Non-sulfur
Enrichment of bacteria culture medium, minimal medium, beef-protein medium, the training for containing high concentration (> 500mg/L) ammonium nitrogen
It supports in base, the volume ratio of waste water and culture medium is 1:20, and culture dish is inverted in (25-35 DEG C) constant temperature incubation 24-48h；From upper
It states and picks them separately the maximum single bacterium colony center portion thallus of volume in plate on the plate of different culture mediums, in nutrient agar
Flat lining out purifying, until being transferred respectively with 2-6% inoculum concentration in nutrient agar slopes after purebred, setting (25-35 DEG C) culture
24-48h, then every bacterial strain is added in sterilized wastewater after expansion culture with 2-6% inoculum concentration and is prepared into composite bacteria agent.
Further, the preparation process of the algae strain is as follows: waste water is diluted step by step, until single form algae strain is obtained,
Then it is expanded culture on BG11 culture medium, until obtaining purifying algae strain.
Further, step (1) medium temperature chamber condition are as follows: temperature is 20.5 DEG C -37 DEG C, relative humidity 36-
86.5%.
Beneficial effects of the present invention are as follows: the present invention carries out restoration of the ecosystem to waste water with production alkali algae-bacterium culture systems first,
Dissolved organic carbon, ammonia nitrogen, phosphorus, heavy metal and pathogeny bacterium are removed, and wastewater pH is made to be increased to 10 or more, then with treated
Acid soil is irrigated in water outlet.Since alkaline waste water can flow into rapidly the soil body, OH in water-It can be with the H in soil+、Al3+And Mn2+Reaction, because
This can improve rapidly soil pH, reduce the content of activated aluminum and manganese, and the algal biomass in water is withheld with other organic matters and is existed
In soil, increase soil fertility.Due to Soil water interactions, the pH of water declines, and algal biomass and dissolved organic carbon can in water
It is completely removed, significantly improves water quality.Compared with the method for individually repairing waste water and acid soil at present, this method has investment
It is low, output is high, do not need chemicals, does not generate the advantage that sediment, sustainability are strong and quality is high.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.Without departing from the spirit and substance of the case in the present invention, to method of the invention, step
Or the modifications or substitutions that condition is done, the scope of the invention is belonged to, unless otherwise specified, technological means used in embodiment is
Conventional means familiar to those skilled in the art.
Embodiment:
The present embodiment repairs Pig raising wastewater (waste water generated in live pig production process) and acid soil with combined remediation method.
(1) preparation of composite bacteria agent: 5mL waste water is taken to be added separately to the Van Niel purple nonsulfur bacteria enrichment of 100mL
Culture medium, beef-protein medium, contains in the culture medium of high concentration (> 500mg/L) ammonium nitrogen minimal medium,
With spreading rod even spread, and culture dish is inverted in 25-35 DEG C of constant temperature incubation 24-48h.From the flat of above-mentioned different culture medium
The maximum single bacterium colony center portion thallus of volume in plate is picked them separately on plate, purifying of crossing on nutrient agar panel, directly
To after purebred, transferred respectively with 5% inoculum concentration in nutrient agar slopes, set 25-35 DEG C of culture 24-48h, then every bacterial strain is with 2-
6% inoculum concentration is prepared into composite bacteria agent in being added in sterilized wastewater after expansion culture.
(2) preparation of algae strain: waste water is diluted step by step (from 10-1To 10-3), three kinds of various concentrations take 0.5ml microscopy,
Until obtaining single form algae strain, then expanded culture on BG11 culture medium, until obtaining purifying algae strain.
(3) phycomycete culture: pig farm is taken treated biogas slurry (waste water i.e. to be processed) in 23L transparent plastic bottle, by algae
Strain and compound bacteria are seeded in the transparent plastic bottle for filling waste water to be processed, wherein the inoculum concentration of compound bacteria is 10mL compound bacteria
Composite bacteria agent/1L waste water, it is 0.05-0.07 that the inoculum concentration of algae strain, which is initial OD 680,.Under solar irradiation, temperature be 20.5 DEG C-
37 DEG C, relative humidity be 36-86.5% under conditions of, cultivate about 33 days；Pass through the assimilation of algae and the biodegrade removal of bacterium
Pollutant, while alkali volatilization ammonia, precipitation of heavy metals and the kill pathogenic bacteria generated by system, until water quality reaches pre- calibration
The pH of standard, water reaches 10 or more, and the water quality parameter for cultivating front and back is shown in Table 1.
For water quality parameter before and after the processing, (data are that the average values of 3 reprocessings add standard deviation to table 1, and O-W is that waste water is former
Sample, 23 liters of the position 23L-A-E phycomycete treated waste water, S-L are the percolate after soil treatment.AD is algae density, and DOC is solvable
Property organic carbon)
(4) irrigation soils: the acidity fetched from one tea place of Hangzhou is irrigated with the phycomycete culture solution of 1.5L/kg soil dosage
Soil, the water quality parameter after Irrigation regime are shown in Table 1, and soil property before and after the processing is shown in Table 2.
Table 2 be with deionized water, lime, after phycomycete culture wastewater treatment 14 days soil property, data be 3 times repetition at
The average value of reason adds standard deviation
| pH | Exchangeable Al mg/kg | Exchangeability Mn mg/kg | |
| Deionized water | 4.92±0.11 | 263.6±11.8 | 41.1±2.4 |
| Lime | 5.66±0.20 | 80.9±4.9 | 18.0±4.1 |
| Phycomycete culture waste water | 6.16±0.16 | 60.4±1.3 | 11.9±1.0 |
Tables 1 and 2 the result shows that, combined remediation method can repair Pig raising wastewater and acid soil simultaneously, useless after reparation
The livestock breeding wastewater discharge standard GB 18596-2001 that Environmental Protection Department is issued, the soil after reparation is fully achieved in water water quality
PH reaches 6 or more, and the content of exchangeable aluminum and manganese is remarkably decreased, therefore repairing quality is satisfactory.
Claims (1)
1. a kind of method that joint repairs waste water and acid soil, which is characterized in that specifically comprise the following steps:
(1) phycomycete culture: algae strain and compound bacteria are seeded in the container waste water to be processed for filling waste water to be processed, in the sun
It is cultivated under illumination and greenhouse experiment, until the pH of water reaches 10 or more in container；The inoculum concentration of the algae strain is initial
OD680 is 0.05-0.07；The inoculum concentration of the compound bacteria is 10mL composite bacteria agent/1L waste water；
The preparation process of the algae strain is as follows: waste water being diluted step by step, until obtaining single form algae strain, is then cultivated in BG11
It is expanded culture on base, until obtaining purifying algae strain；
The preparation process of the composite bacteria agent is as follows: waste water being taken to be added separately to Van Niel purple nonsulfur bacteria enrichment culture
Base, minimal medium, beef-protein medium, in the culture medium containing high concentration ammonium nitrogen, the concentration of ammonium nitrogen >
The volume ratio of 500mg/L, waste water and culture medium is 1:20, and culture dish is inverted in 25-35 DEG C of constant temperature incubation 24-48 h；From upper
It states and picks them separately the maximum single bacterium colony center portion thallus of volume in plate on the plate of different culture mediums, in nutrient agar
Flat lining out purifying, until being transferred respectively with 2-6% inoculum concentration in nutrient agar slopes after purebred, setting 25-35 DEG C of culture 24-
48 h, then every bacterial strain is added in sterilized wastewater after expansion culture with 2-6% inoculum concentration and is prepared into composite bacteria agent；
Greenhouse experiment are as follows: temperature is 20.5 °C -37 °C, and relative humidity is 36-86.5 %；
(2) irrigation soils: acid soil is irrigated with the water that phycomycete culture is handled, the pH of soil is made to reach 6 or more.
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| CN201611206579.2A CN106623406B (en) | 2016-12-23 | 2016-12-23 | A method of joint repairs waste water and acid soil |
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| CN106623406B true CN106623406B (en) | 2019-10-29 |
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| WO2021243625A1 (en) * | 2020-06-04 | 2021-12-09 | 浙江大学 | Preparation method for algae powder and use of algae powder in improvement of desert soil |
| CN115490332B (en) * | 2022-09-05 | 2024-03-29 | 湖南农业大学 | Method for improving pH value of acidic water/soil by utilizing crusted algae |
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