CN106617050A - Fucoxanthin nano-emulsion and preparation method Thereofthereof - Google Patents
Fucoxanthin nano-emulsion and preparation method Thereofthereof Download PDFInfo
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- CN106617050A CN106617050A CN201610980586.1A CN201610980586A CN106617050A CN 106617050 A CN106617050 A CN 106617050A CN 201610980586 A CN201610980586 A CN 201610980586A CN 106617050 A CN106617050 A CN 106617050A
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- fucoxanthin
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- SJWWTRQNNRNTPU-ABBNZJFMSA-N fucoxanthin Chemical compound C[C@@]1(O)C[C@@H](OC(=O)C)CC(C)(C)C1=C=C\C(C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)C(=O)C[C@]1(C(C[C@H](O)C2)(C)C)[C@]2(C)O1 SJWWTRQNNRNTPU-ABBNZJFMSA-N 0.000 title claims abstract description 67
- AQLRNQCFQNNMJA-UHFFFAOYSA-N fucoxanthin Natural products CC(=O)OC1CC(C)(C)C(=C=CC(=CC=CC(=CC=CC=C(/C)C=CC=C(/C)C(=O)CC23OC2(C)CC(O)CC3(C)C)C)CO)C(C)(O)C1 AQLRNQCFQNNMJA-UHFFFAOYSA-N 0.000 title claims abstract description 67
- 239000007908 nanoemulsion Substances 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000003381 stabilizer Substances 0.000 claims abstract description 31
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 22
- 239000002904 solvent Substances 0.000 claims abstract description 22
- 239000003921 oil Substances 0.000 claims description 89
- 235000019198 oils Nutrition 0.000 claims description 89
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 69
- 239000000203 mixture Substances 0.000 claims description 15
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
- 239000011435 rock Substances 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- -1 sucrose ester Chemical class 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000000839 emulsion Substances 0.000 claims description 3
- 159000000000 sodium salts Chemical class 0.000 claims description 3
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 2
- KKAJSJJFBSOMGS-UHFFFAOYSA-N 3,6-diamino-10-methylacridinium chloride Chemical compound [Cl-].C1=C(N)C=C2[N+](C)=C(C=C(N)C=C3)C3=CC2=C1 KKAJSJJFBSOMGS-UHFFFAOYSA-N 0.000 claims description 2
- 235000005979 Citrus limon Nutrition 0.000 claims description 2
- 244000131522 Citrus pyriformis Species 0.000 claims description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- 235000019486 Sunflower oil Nutrition 0.000 claims description 2
- 235000010323 ascorbic acid Nutrition 0.000 claims description 2
- 239000011668 ascorbic acid Substances 0.000 claims description 2
- 229960005070 ascorbic acid Drugs 0.000 claims description 2
- 235000005687 corn oil Nutrition 0.000 claims description 2
- 239000002285 corn oil Substances 0.000 claims description 2
- 229960001484 edetic acid Drugs 0.000 claims description 2
- 210000002969 egg yolk Anatomy 0.000 claims description 2
- 150000003904 phospholipids Chemical class 0.000 claims description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 2
- 235000013824 polyphenols Nutrition 0.000 claims description 2
- 229920000136 polysorbate Polymers 0.000 claims description 2
- JAJWGJBVLPIOOH-IZYKLYLVSA-M sodium taurocholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 JAJWGJBVLPIOOH-IZYKLYLVSA-M 0.000 claims description 2
- 229940083466 soybean lecithin Drugs 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- 239000002600 sunflower oil Substances 0.000 claims description 2
- 241000195493 Cryptophyta Species 0.000 claims 2
- 244000269722 Thea sinensis Species 0.000 claims 1
- 235000014113 dietary fatty acids Nutrition 0.000 claims 1
- 229930195729 fatty acid Natural products 0.000 claims 1
- 239000000194 fatty acid Substances 0.000 claims 1
- 125000005456 glyceride group Chemical group 0.000 claims 1
- 235000015112 vegetable and seed oil Nutrition 0.000 claims 1
- 239000008158 vegetable oil Substances 0.000 claims 1
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 239000012071 phase Substances 0.000 abstract 8
- 239000008346 aqueous phase Substances 0.000 abstract 2
- 230000000694 effects Effects 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000001681 protective effect Effects 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 235000021466 carotenoid Nutrition 0.000 description 2
- 150000001747 carotenoids Chemical class 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- BGHCVCJVXZWKCC-UHFFFAOYSA-N tetradecane Chemical compound CCCCCCCCCCCCCC BGHCVCJVXZWKCC-UHFFFAOYSA-N 0.000 description 2
- ZGONASGBWOJHDD-UHFFFAOYSA-N 1-ethyl-2-nitro-1-nitrosoguanidine Chemical compound CCN(N=O)C(N)=N[N+]([O-])=O ZGONASGBWOJHDD-UHFFFAOYSA-N 0.000 description 1
- 208000031261 Acute myeloid leukaemia Diseases 0.000 description 1
- 241000199899 Alariaceae Species 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 206010061825 Duodenal neoplasm Diseases 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
- 208000002454 Nasopharyngeal Carcinoma Diseases 0.000 description 1
- 206010061306 Nasopharyngeal cancer Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 1
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
- 241000015177 Saccharina japonica Species 0.000 description 1
- 206010054184 Small intestine carcinoma Diseases 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 241001261505 Undaria Species 0.000 description 1
- 241001261506 Undaria pinnatifida Species 0.000 description 1
- 206010046851 Uveitis Diseases 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000010495 camellia oil Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 201000000312 duodenum cancer Diseases 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 201000011216 nasopharynx carcinoma Diseases 0.000 description 1
- 229960003104 ornithine Drugs 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000003334 potential effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses fucoxanthin nano-emulsion and a preparation method Thereofthereof. The preparation method of the fucoxanthin nano-emulsion comprises the following steps; step 1), taking fucoxanthin and dissolving the taken fucoxanthin into a solvent at 30-70 DEG C so as to prepare fucoxanthin oil, wherein the mass-volume fraction of the fucoxanthin in the solvent is 0.5%~-50%; step 2), mixing the fucoxanthin oil with an oil phase so as to form a first mixed oil phase, wherein the percentage by weight of the fucoxanthin oil in the first mixed oil phase is 0.1%--45%; steps 3), adding an emulsifier into the first mixed oil phase and carrying out uniform mixing so as to form a second mixed oil phase, wherein the temperature of the first mixed oil phase is controlled at 30-55 DEG C and the weight ratio of the emulsifier to the first mixed oil phase is 3: 4; and 4), adding an aqueous phase containing a water-soluble stabilizer into the second mixed oil phase and carrying out uniform mixing, wherein the weight of the aqueous phase containing the water-soluble stabilizer accounts for 91-95.8% of the total weight. The fucoxanthin nano-emulsion has improved fucoxanthin stability, and is small in energy consumption and low in cost, so that the fucoxanthin nano-emulsion is suitable for llarge-scale promotion.
Description
Technical field
The present invention relates to the preparation method of fucoxanthin carrier, in particular, be a kind of fucoxanthin nanoemulsions and
Its preparation method.
Background technology
Fucoxanthine (fucoxanthin) is also known as brown alga flavine, be from edible brown alga, such as undaria pinnitafida (Alariaceae,
Undaria pinnatifida), the natural carotenoids that extract in sea-tangle (Laminaria japonica Aresch)
Element, has respectively active 5, the 6- epoxy unsaturation allene bond of a chemical property at the two ends of its rigid alltrans long-chain
Structure, thus and different from other carotenoid molecules, with very strong biologically active.In recent years, its various biological is active
It is proved, among some potential activity are also actively being sought by scientists, at present oneself becomes current marine drug research
With one of the main attack focus of exploitation.
Fucoxanthine has the multiple biological activities such as antitumor, anti-inflammatory, anti-oxidant, fat-reducing.First, in anti-tumor aspect,
The reported firsts such as nineteen ninety Okuzumi, the μ g/mL of fucoxanthine 10 can reduce by 62% Ct Characteristics of Nasopharyngeal Carcinoma Involving The Pterygopatatine after being incubated 3 days
The propagation of cell line (GOTO).The research such as Okuztllni confirms that fucoxanthine can suppress to promote cancer thing tetradecane phthalein Buddhist by strong skin
The de- shuttle enzymatic activity of mouse skin ornithine of ripple acetic acid cruel (TPA) induction strengthens, and speculates that it may have suppression to cutaneum carcinoma accordingly
Effect.The report such as okuzumi in 1993, duodenal cancer shape of the fucoxanthine to N- ethyl-N '-nitro-nitroso guanidine induction
Into inhibited.Masashi etc. reports effect of the fucoxanthine to acute myeloid leukaemia HL-60 cell lines, as a result
Show that fucoxanthine can play significant inhibited proliferation to HL-60 cells.The research such as Elichi finds that fucoxanthine can be bright
It is aobvious to reduce prostate cancer cell survival rate, and inducing cell apoptosis.Swadesh K et al. researchs find fucoxanthine to human liver cancer
The growth of HePG2 cells is inhibited, therefore, there is fucoxanthine different degrees of suppression to make to kinds of tumor cells
With.Secondly, research finds that fucoxanthine also has antioxidation activity in vitro, and its antioxidation activity is even stronger than vitamin C and E.This
Outward, Kenjis etc. has found that matter suppresses the rat uveitis (Elu) of endotaxin induction, and its antiinflammatory action and Ni Songlong phases
When.Particularly Recent study finds, fucoxanthine can significant burn fat cell, the effect of elimination fat accumulation, from
And significant fat-reducing effect is played, more make fucoxanthine that consequence is occupied in slimming drugs market, therefore, fucoxanthine is
One broad-spectrum marine active substance.
But fucoxanthine non-refractory high pressure, so the application of fucoxanthine is restricted.In system in prior art
When having pass fucoxanthine carrier, typically fucoxanthine oil is dissolved in into acetone and other organic solvent, and adopt high pressure homogenizer with
And Ultrasonic Cell Disruptor is from extraneous input energy, because fucoxanthine does not tolerate in itself HTHP, so with especially violent bar
Part production can cause the loss in production process.
If not using the said equipment, can directly be chelated with large biological molecules such as cyclodextrin or fucoxanthine is directly molten
Solution in the organic solvent that can be applied in food or health products, such as ethanol.But after large biological molecule chelating
Protective effect to fucoxanthine is not fine, because the porous of structure of biological macromolecule, secondly large biological molecule is in storage
It is unstable during depositing；Ethanol is readily volatilized, and preparation can be caused unstable, and is not very to the protective effect of fucoxanthine
By force, in addition solubility of the fucoxanthine in water is relatively low, causes its absorptivity and bioavilability in the gastrointestinal tract not high, together
Easily there is isomery and degraded under the environment such as Shi Guang, soda acid, cause its stability poor.
The content of the invention
It is not enough in order to make up the above, the invention provides a kind of fucoxanthin nanoemulsions and its preparation are taken precautions against, to solve
The problem that fucoxanthine solubility in water is low and its stability is not high in above-mentioned background technology.
The solution of the present invention is：
A kind of preparation method of fucoxanthin nanoemulsions, including step in detail below：
Step 1) take fucoxanthin solvent is incorporated under the conditions of 30～70 DEG C and make fucoxanthin oil, the fucoxanthin exists
Quality volume fraction in solvent is 0.5%～50%；
Step 2) by step 1) in made by fucoxanthin oil mix with oil phase after the mixing oil phase of formation first, and the rock
The weight of phycoxanthine oil is the 0.1%～45% of the first mixing oil phase weight；
Step 3) by step 2) in made by add emulsifying agent to be well mixed to form the second miscella in the first mixing oil phase
Phase, at 30～55 DEG C, it is 3 that the emulsifying agent mixes the weight ratio of oil phase with described first to the first miscella temperature control:
4；
Step 4) to step 3) in made by add the water phase containing water soluble stabilizer in the second mixing oil phase, stirring is equal
It is even, wherein the water containing water soluble stabilizer mutually accounts for the 91%～95.8% of gross weight.
Used as preferred scheme, the solvent is the one of which of tea oil, sunflower oil, corn oil and lemon.
As preferred scheme, the emulsifying agent be soybean lecithin, yolk phospholipid, sodium taurocholate, Bile Salts, tween and
The mixture of one or more in sucrose ester.
Used as preferred scheme, the water is mutually using distilled water or distilled water and glycerol mixture product.
Used as preferred scheme, the oil phase is the mixed of one or two in straight-chain fatty acid ester and fatty glyceride
Compound.
As preferred scheme, the step 4) in, add in the described second mixing oil phase and contain water soluble stabilizer
Water phase when, the water containing water soluble stabilizer is mutually added dropwise over.
Used as preferred scheme, the water soluble stabilizer is ascorbic acid or its sodium salt, ethylenediamine tetra-acetic acid or its sodium
The mixture of one or more in salt, Tea Polyphenols.
As preferred scheme, quality of the water soluble stabilizer in water phase in the water phase containing water soluble stabilizer
Volume fraction is 0.0005%～10%.
The present invention also provides a kind of fucoxanthin nanoemulsions, is the first mixing oil phase, emulsifying agent, stablizes containing water-soluble
The oil-in-water shape emulsion that the water of agent is mutually formed, wherein, the water containing water soluble stabilizer mutually accounts for the 91%～95.8% of gross weight,
The first mixing oil phase, the weight ratio of emulsifying agent are 4: 3；The first mixing oil phase includes salt phycoxanthine oil and oil phase, and
The weight of the fucoxanthin oil is the 0.1%～45% of the first mixing oil phase weight, and the fucoxanthin oil includes salt
Phycoxanthine and solvent, and fucoxanthin quality volume fraction in a solvent is 0.5%～50%.
As preferred scheme, quality of the water soluble stabilizer in water phase in the water phase containing water soluble stabilizer
Volume fraction is 0.0005%～10%.
As a result of above-mentioned technical proposal, a kind of preparation method of fucoxanthin nanoemulsions, including walking in detail below
Suddenly：Step 1) take fucoxanthin incorporate under the conditions of 30～75 DEG C solvent make fucoxanthin oil, the fucoxanthin is in solvent
In quality volume fraction be 0.5%～50%；Step 2) by step 1) in made by fucoxanthin oil mix with oil phase after shape
Into the first mixing oil phase, and the weight of fucoxanthin oil is the 0.1%～45% of the first mixing oil phase weight；Step
3) by step 2) in made by first mixing oil phase in add emulsifying agent be well mixed to form the second mixing oil phase, described first mix
Close oil temperature to control at 30～55 DEG C, it is 3: 4 that the emulsifying agent mixes the weight ratio of oil phase with described first；Step 4) to step
3) the water phase containing water soluble stabilizer is added in the second mixing oil phase made by, is stirred, wherein containing water-soluble steady
The water for determining agent mutually accounts for the 91%～95.8% of gross weight.
Advantages of the present invention：A process for preparing the oil-in-water type nano-emulsion liquid that particle is tiny, long-term dynamics are stable, and
Energy consumption is little, low cost, without HTHP and need not using high pressure homogenizer or sonicator it is same from extraneous input energy
When hydrophobic oil phase and fucoxanthin oil are mixed well, fucoxanthin is embedded in closely spherical emulsion, can be fine
Protective effect is provided to fucoxanthin.
Specific embodiment
In order that technological means, creation characteristic, reached purpose and effect that the present invention is realized are easy to understand, tie below
Specific embodiment is closed, the present invention is expanded on further.
Embodiment one：
Step 1) take fucoxanthin incorporate under the conditions of 30 DEG C solvent make fucoxanthin oil, the fucoxanthin is in solvent
In quality volume fraction be 0.5%；
Step 2) by step 1) in made by fucoxanthin oil mix with oil phase after the mixing oil phase of formation first, and the rock
The weight of phycoxanthine oil is the 0.1%% of the first mixing oil phase weight；
Step 3) by step 2) in made by add emulsifying agent to be well mixed to form the second miscella in the first mixing oil phase
Phase, at 30 DEG C, it is 3: 4 that the emulsifying agent mixes the weight ratio of oil phase with described first to the first miscella temperature control；
Step 4) to step 3) in made by add the water phase containing water soluble stabilizer in the second mixing oil phase, stirring is equal
It is even, wherein the water containing water soluble stabilizer mutually accounts for the 91% of gross weight.
Embodiment two：
Step 1) take fucoxanthin incorporate under the conditions of 70 DEG C solvent make fucoxanthin oil, the fucoxanthin is in solvent
In quality volume fraction be 50%；
Step 2) by step 1) in made by fucoxanthin oil mix with oil phase after the mixing oil phase of formation first, and the rock
The weight of phycoxanthine oil is the 45% of the first mixing oil phase weight；
Step 3) by step 2) in made by add emulsifying agent to be well mixed to form the second miscella in the first mixing oil phase
Phase, at 30～55 DEG C, it is 3 that the emulsifying agent mixes the weight ratio of oil phase with described first to the first miscella temperature control:
4；
Step 4) to step 3) in made by add the water phase containing water soluble stabilizer in the second mixing oil phase, stirring is equal
It is even, wherein the water containing water soluble stabilizer mutually accounts for the 95.8% of gross weight.
Embodiment three：
Step 1) take fucoxanthin incorporate under the conditions of 30 DEG C solvent make fucoxanthin oil, the fucoxanthin is in solvent
In quality volume fraction be 5%；
Step 2) by step 1) in made by fucoxanthin oil mix with oil phase after the mixing oil phase of formation first, and the rock
The weight of phycoxanthine oil is the 5% of the first mixing oil phase weight；
Step 3) by step 2) in made by add emulsifying agent to be well mixed to form the second miscella in the first mixing oil phase
Phase, at 50 DEG C, it is 3: 4 that the emulsifying agent mixes the weight ratio of oil phase with described first to the first miscella temperature control；
Step 4) to step 3) in made by add the water phase containing water soluble stabilizer in the second mixing oil phase, stirring is equal
It is even, wherein the water containing water soluble stabilizer mutually accounts for the 94% of gross weight.
Example IV：
Step 1) take fucoxanthin incorporate under the conditions of 35 DEG C solvent make fucoxanthin oil, the fucoxanthin is in solvent
In quality volume fraction be 1%；
Step 2) by step 1) in made by fucoxanthin oil mix with oil phase after the mixing oil phase of formation first, and the rock
The weight of phycoxanthine oil is the 2% of the first mixing oil phase weight；
Step 3) by step 2) in made by add emulsifying agent to be well mixed to form the second miscella in the first mixing oil phase
Phase, at 55 DEG C, it is 3: 4 that the emulsifying agent mixes the weight ratio of oil phase with described first to the first miscella temperature control；
Step 4) to step 3) in made by add the water phase containing water soluble stabilizer in the second mixing oil phase, stirring is equal
It is even, wherein the water containing water soluble stabilizer mutually accounts for the 95% of gross weight.
General principle, principal character and advantages of the present invention that the above only depicts the present invention.The skill of the industry
Simply explanation of the art personnel it should be appreciated that the present invention is not restricted to the described embodiments, described in above-described embodiment and specification
The principle of the present invention, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, these
Changes and improvements are both fallen within scope of the claimed invention.The claimed scope of the invention by appending claims and
Its equivalent circle..
Claims (10)
1. a kind of preparation method of fucoxanthin nanoemulsions, it is characterised in that including step in detail below：
Step 1) take fucoxanthin incorporate under the conditions of 30～70 DEG C solvent make fucoxanthin oil, the fucoxanthin is in solvent
In quality volume fraction be 0.5%～50%；
Step 2) by step 1) in made by fucoxanthin oil mix with oil phase after form the first mixing oil phase, and rock algae Huang
The weight of vegetable oil is the 0.1%～45% of the first mixing oil phase weight；
Step 3) by step 2) in made by add emulsifying agent to be well mixed to form the second mixing oil phase, institute in the first mixing oil phase
The first miscella temperature control is stated at 30～55 DEG C, it is 3: 4 that the emulsifying agent mixes the weight ratio of oil phase with described first；
Step 4) to step 3) in made by second mixing oil phase in add the water phase containing water soluble stabilizer, stir,
Wherein the water containing water soluble stabilizer mutually accounts for the 91%～95.8% of gross weight.
2. a kind of preparation method of fucoxanthin nanoemulsions as claimed in claim 1, it is characterised in that the solvent is tea
The one of which of tree oil, sunflower oil, corn oil and lemon.
3. a kind of preparation method of fucoxanthin nanoemulsions as claimed in claim 1, it is characterised in that：The emulsifying agent is
The mixture of one or more in soybean lecithin, yolk phospholipid, sodium taurocholate, Bile Salts, tween and sucrose ester.
4. a kind of preparation method of fucoxanthin nanoemulsions as claimed in claim 1, it is characterised in that：The water is mutually adopted
Distilled water or distilled water and glycerol mixture product.
5. a kind of preparation method of fucoxanthin nanoemulsions as claimed in claim 1, it is characterised in that：The oil phase is straight
One or two mixture in chain fatty acid ester and fatty glyceride.
6. a kind of preparation method of fucoxanthin nanoemulsions as claimed in claim 1, it is characterised in that：The step 4)
In, when adding the water phase containing water soluble stabilizer in the described second mixing oil phase, the water containing water soluble stabilizer
Mutually it is added dropwise over.
7. a kind of preparation method of fucoxanthin nanoemulsions as claimed in claim 1, it is characterised in that：It is described water-soluble steady
Agent is determined for the mixture of one or more in ascorbic acid or its sodium salt, ethylenediamine tetra-acetic acid or its sodium salt, Tea Polyphenols.
8. a kind of preparation method of salt phycoxanthine nanoemulsions as claimed in claim 1, it is characterised in that：It is described containing water-soluble
Property stabilizer water phase in quality volume fraction of the water soluble stabilizer in water phase be 0.0005%～10%.
9. a kind of fucoxanthin nanoemulsions, it is characterised in that：For the first mixing oil phase, emulsifying agent, contain water soluble stabilizer
The oil-in-water shape emulsion that mutually formed of water, wherein, the water containing water soluble stabilizer mutually accounts for the 91%～95.8% of gross weight, institute
State the first mixing oil phase, the weight ratio of emulsifying agent is 4: 3；The first mixing oil phase includes salt phycoxanthine oil and oil phase, and institute
The weight for stating fucoxanthin oil is the 0.1%～45% of the first mixing oil phase weight, and the fucoxanthin oil includes salt algae
Flavine and solvent, and fucoxanthin quality volume fraction in a solvent is 0.5%～50%.
10. a kind of fucoxanthin nanoemulsions as claimed in claim 9, it is characterised in that：It is described containing water soluble stabilizer
Water phase in quality volume fraction of the water soluble stabilizer in water phase be 0.0005%～10%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109588711A (en) * | 2019-01-25 | 2019-04-09 | 集美大学 | A kind of fucoxanthin-oyster peptide nanoparticles and its preparation method and application |
| CN115708787A (en) * | 2022-11-18 | 2023-02-24 | 大连工业大学 | Emulsion of fucoxanthin for inhibiting melanin, preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1164387A (en) * | 1996-03-11 | 1997-11-12 | 巴斯福股份公司 | Stable carotenoid emulsions suitable for parenteral administration |
| KR20090096256A (en) * | 2008-03-07 | 2009-09-10 | 주식회사 코씨드바이오팜 | A method for producing extract of fermented fucoxanthin and Cosmetic composition containing thereof |
| CN102892414A (en) * | 2010-05-18 | 2013-01-23 | 富士胶片株式会社 | Carotenoid-containing composition and production method therefor |
| CN103379893A (en) * | 2011-03-30 | 2013-10-30 | 富士胶片株式会社 | Carotenoid-containing composition and method for producing same |
| CN104042568A (en) * | 2014-06-23 | 2014-09-17 | 青岛优度生物工程有限公司 | Astaxanthin nanoemulsion preparation and preparation method thereof |
| CN105078894A (en) * | 2015-07-13 | 2015-11-25 | 桂林莱茵生物科技股份有限公司 | Preparation method of water-soluble fucoxanthine dry powder |
-
2016
- 2016-11-08 CN CN201610980586.1A patent/CN106617050A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1164387A (en) * | 1996-03-11 | 1997-11-12 | 巴斯福股份公司 | Stable carotenoid emulsions suitable for parenteral administration |
| KR20090096256A (en) * | 2008-03-07 | 2009-09-10 | 주식회사 코씨드바이오팜 | A method for producing extract of fermented fucoxanthin and Cosmetic composition containing thereof |
| CN102892414A (en) * | 2010-05-18 | 2013-01-23 | 富士胶片株式会社 | Carotenoid-containing composition and production method therefor |
| CN103379893A (en) * | 2011-03-30 | 2013-10-30 | 富士胶片株式会社 | Carotenoid-containing composition and method for producing same |
| CN104042568A (en) * | 2014-06-23 | 2014-09-17 | 青岛优度生物工程有限公司 | Astaxanthin nanoemulsion preparation and preparation method thereof |
| CN105078894A (en) * | 2015-07-13 | 2015-11-25 | 桂林莱茵生物科技股份有限公司 | Preparation method of water-soluble fucoxanthine dry powder |
Non-Patent Citations (3)
| Title |
|---|
| 徐丽青等: "高压微射流制备岩藻黄素纳米乳液的影响因素及其理化分析", 《食品科学》 * |
| 王迪等: "β-胡萝卜素乳状液研究进展", 《中国食品添加剂》 * |
| 陈晓利等: "岩藻黄素在水、油模型体系中稳定性的研究", 《食品工业科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109588711A (en) * | 2019-01-25 | 2019-04-09 | 集美大学 | A kind of fucoxanthin-oyster peptide nanoparticles and its preparation method and application |
| CN115708787A (en) * | 2022-11-18 | 2023-02-24 | 大连工业大学 | Emulsion of fucoxanthin for inhibiting melanin, preparation method and application thereof |
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