CN106615629A - Bee larva probiotic oral liquid for animal healthcare - Google Patents
Bee larva probiotic oral liquid for animal healthcare Download PDFInfo
- Publication number
- CN106615629A CN106615629A CN201611205874.6A CN201611205874A CN106615629A CN 106615629 A CN106615629 A CN 106615629A CN 201611205874 A CN201611205874 A CN 201611205874A CN 106615629 A CN106615629 A CN 106615629A
- Authority
- CN
- China
- Prior art keywords
- animal
- queen bee
- oral liquid
- fermentation
- bee tire
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001465754 Metazoa Species 0.000 title claims abstract description 35
- 239000007788 liquid Substances 0.000 title claims abstract description 22
- 239000006041 probiotic Substances 0.000 title abstract description 8
- 235000018291 probiotics Nutrition 0.000 title abstract description 8
- 230000000529 probiotic effect Effects 0.000 title abstract description 4
- 238000000855 fermentation Methods 0.000 claims abstract description 20
- 230000004151 fermentation Effects 0.000 claims abstract description 16
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 26
- 241000894006 Bacteria Species 0.000 claims description 22
- 230000036541 health Effects 0.000 claims description 15
- 235000014655 lactic acid Nutrition 0.000 claims description 13
- 239000004310 lactic acid Substances 0.000 claims description 13
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 12
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 9
- 239000008101 lactose Substances 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000009928 pasteurization Methods 0.000 claims description 5
- 241000186000 Bifidobacterium Species 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 210000000936 intestine Anatomy 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 235000013406 prebiotics Nutrition 0.000 claims description 4
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 238000010564 aerobic fermentation Methods 0.000 claims description 3
- 241000194108 Bacillus licheniformis Species 0.000 claims description 2
- 241000194032 Enterococcus faecalis Species 0.000 claims description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 2
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 2
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 2
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 2
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 2
- 206010012735 Diarrhoea Diseases 0.000 abstract description 7
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 6
- 102000004169 proteins and genes Human genes 0.000 abstract description 6
- 108090000623 proteins and genes Proteins 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 3
- 239000003674 animal food additive Substances 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 239000000796 flavoring agent Substances 0.000 abstract 2
- 235000019634 flavors Nutrition 0.000 abstract 2
- 239000013543 active substance Substances 0.000 abstract 1
- 230000000975 bioactive effect Effects 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 235000012907 honey Nutrition 0.000 abstract 1
- 230000000717 retained effect Effects 0.000 abstract 1
- 241000256844 Apis mellifera Species 0.000 description 45
- 238000012360 testing method Methods 0.000 description 14
- 102000004641 Fetal Proteins Human genes 0.000 description 4
- 108010003471 Fetal Proteins Proteins 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 230000000968 intestinal effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 229920002101 Chitin Polymers 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000013401 experimental design Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000007413 intestinal health Effects 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 229940109850 royal jelly Drugs 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 206010002198 Anaphylactic reaction Diseases 0.000 description 1
- 241000256837 Apidae Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 241000193468 Clostridium perfringens Species 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 241000293871 Salmonella enterica subsp. enterica serovar Typhi Species 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 241000607764 Shigella dysenteriae Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 230000002009 allergenic effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000036783 anaphylactic response Effects 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 238000003975 animal breeding Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000009341 apiculture Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000014590 basal diet Nutrition 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000006189 buccal tablet Substances 0.000 description 1
- 229940046011 buccal tablet Drugs 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 230000032677 cell aging Effects 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 230000004992 fission Effects 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 201000007094 prostatitis Diseases 0.000 description 1
- 210000000664 rectum Anatomy 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 229940007046 shigella dysenteriae Drugs 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/515—Animalis
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A bee larva probiotic oral liquid for animal healthcare is prepared by fermenting bee larva homogenate pretreated with animal probiotics disclosed in Lists of Feed Additives by Department of Agriculture, the original nutritional composition and functional active substances in bee honey are retained, and the bee larva proteins are fermented by animal probiotics and hydrolyzed into small-molecular bioactive peptide so that the health-care function of the oral liquid is enhanced. In addition, rich flavor matters are produced during fermentation, the flavor is improved, and good attracting effect is provided. Animal experiments prove that the bee larva probiotic oral liquid for animal healthcare is capable of lowering the chances of piglet diarrhea significantly and decreasing feed-gain ratio and input-gain ratio significantly, and average daily weight increase is increased.
Description
Technical field
The invention discloses a kind of animal health queen bee tire probio oral liquid, belongs to feed addictive and animal-breeding
Field.
Background technology
Queen bee tire, also known as queen bee nit, is that the embryonated egg Jing workers bee of honeybee are fed with the larva that new fresh royal jelly grows
Body.Nutritional studies show that queen bee tire is rich in protein, amino acid, chitin polysaccharide, vitamin, trace element, enzyme, nucleic acid, Huang
Ketone etc., nutritive value is high.Bioactivity research shows that queen bee mold has suppression or delaying cell aging, enhancing organism to exempt from
The health-care efficacies such as epidemic disease function.Therefore, it is a kind of natural health resource of worth Devoting Major Efforts To Developing.
China is time-honored bee-keeping big country, and bee colony number and bee product yield are world prostatitis, have possessed 800 at present
Many ten thousand groups of honeybees, queen bee tire annual production is up to thousands of tons of.But used as the byproduct of production royal jelly, the whole nation is annual to be processed profit
Queen bee tire only has 50 tons or so, causes the significant wastage of resource.In food processing field, minority is had at present to queen bee tire
Developmental research, such as University Of Agriculture and Forestry In Fujian Liu Xiu is beautiful and Zhu Haibo is also carried out to the preparation of queen bee fetus oral liquid and buccal tablet
Corresponding research.Domestic patent ZL93100883.2 and CN01107658.5 disclose respectively the related invention of queen bee foetus wine.And incite somebody to action
Queen bee tire is used for field of fodder, there is not yet relevant report.
The invention discloses a kind of animal health queen bee tire probio oral liquid based on the queen bee tire that ferments.Queen bee tire
After being fermented, protein is broken down into small-molecular peptides with amino acid, is more beneficial for human consumption's absorption, and overcomes queen bee tire
The problem of the easy thermal denaturation of protein.Importantly, fermentation energy makes the acid amides bond fission of anaphylactogen, its molecular mass is reduced,
And then reduction anaphylaxis;Fermentation can also change the tertiary structure of allergen epitope so as to be blanked or eliminate so as to reduce sensitization
Property.Additionally, queen bee tire probio oral liquid also has two important functions to ensure intestine of young pigs health, reduce diarrhoea:1)Wherein
Bacillus in animal body especially enteron aisle back segment can produce a large amount of endogenous enzymes, enteron aisle leading portion can not digested completely
Nutrient fully carries out secondary digestion, to improve digesting and assimilating for nutrient, it is to avoid intestines back segments(Such as colon, rectum)Eutrophy
Change, it is to avoid the breeding of harmful bacteria here produces toxin, affects body health;2)Peptides antibacterial is produced after the hydrolysis of queen bee fetoprotein
Matter, can directly kill such as C.perfringens harmful bacteria, it is ensured that intestinal health.
For health care of the animal probio to animal, academic circles at present is reached common understanding.Research shows that animal is prebiotic
Bacterium is entered after animal intestinal, on the one hand can promote the propagation of intrinsic beneficial bacterium in enteron aisle, on the other hand can effectively suppress grape
The pathogenic bacteria such as coccus, candida albicans, Pseudomonas aeruginosa, Escherichia coli, shigella dysenteriae and Salmonella typhi and the breeding of spoilage organisms, so as to
Ensure animal intestinal health.Therefore, people develop a series of treatments or prevent all kinds of animal probiotics preparations of intestines problem.
But have not yet to see the research and development of the animal probiotic products with queen bee fetoprotein as fermentation substrate.
The content of the invention
Its health-care effect and biological characteristics are waved for fully exploitation queen bee tire resource concurrence, the present invention provides a kind of animal and protects
Strong to use queen bee tire probio oral liquid, its preparation process is as follows:
1 adds lactose in queen bee tire homogenate, and pasteurization is carried out after mixing.Wherein Inclusion of Lactose is in mass ratio:Honeybee
King's tire homogenate:Lactose=100:1~100:2;Pasteurisation parameter is 55-60 DEG C, minute time 25-30.
2 per milliliter of pretreated queen bee tire homogenates add animal probio based on total plate count 105~107 CFU.It is dynamic
Thing probio includes bacillus(It is aerobic)The bacillus licheniformis and bacillus subtilis of class, and lactic acid bacteria(Anaerobism)Class
Enterococcus faecalis, lactoenterococcus, lactobacillus acidophilus, Lactobacillus plantarum, animal bifidobacteria.Fermented bacterium is above-mentioned bud in use
At least include a kind of bacillus and a kind of lactic acid bacteria in spore bacillus and the combination of lactic acid bacteria, i.e. fermented bacterium.
3 carry out aerobic fermentation, and round sealed thermal insulating, this stage does not ventilate in addition, Bacillus(It is aerobic)Using and disappear
The oxygen in round is consumed, is next stage to cause anaerobic environment.30 ~ 35 DEG C of fermentation temperature, 16 ~ 24h of fermentation time.
4 carry out anaerobic fermentation, lactic acid bacteria(Anaerobism)Being consumed in the round of oxygen in early stage carries out anaerobism
Ferment.35 ~ 37 DEG C of fermentation temperature, 18 ~ 20h of fermentation time.
The principle of institute's foundation of the present invention is:Add appropriate fermentable saccharide after queen bee tire is homogenized(Lactose), using agricultural
Portion《Catalogue of feed additive varieties》The animal probio of middle announcement is fermented to pretreated queen bee tire homogenate, early stage
During aerobic fermentation, breaks down proteins into small-molecular peptides, are generated biologically active peptide by bacillus, while allergenic protein of degrading.
Under anaerobic, lactic acid bacteria can growth and breeding wherein so as to ferment, carbohydrate is hydrolyzed into into lactic acid etc., remove polypide fishy smell.
Meanwhile, inherently a kind of important beneficial bacteria of intestinal tract of lactic acid bacteria reaches animal intestinal position after intake, there is regulating intestinal canal flora
The effect of balance.
Compared with traditional simple animal probiotics preparation, beneficial effects of the present invention have it is following some:
(1)Queen bee fetoprotein by fermentation after, albumen therein is broken down into amino acid and small peptide, it is easier to inhaled by animal
Receive;
(2)Queen bee tire itself contained bioactivator such as chitin polysaccharide, vitamin, trace element, nucleic acid, flavones etc. are able to
Retain and become a part for prebiotics.
(3)First double section fermentation methods of aerobic anaerobism again, on the one hand allow Bacillus and lactic acid bacteria all sufficiently to be grown generation
Thank, on the other hand save extra anaerobic environment regulation and control, reduce preparation cost.
Specific embodiment
Technical scheme is further elaborated with reference to embodiment.
Embodiment 1
The step of present embodiments providing a kind of animal health queen bee tire probio oral liquid, the present embodiment is as follows:
(1)Pretreatment:Weigh 6kg queen bee tire homogenates, add 97g lactose, pasteurize 30 minutes at 55 DEG C after mixing.
(2)Pretreated queen bee tire homogenate is cooled to into less than 40 DEG C, the animal of the CFU/g of 6g 1.5 × 106 is added
The bacillus subtilis of Bifidobacterium powder, the CFU/g lactoenterococcus powder of 3 g 3.2 × 106 and 1 × 107CFU/g of 4g,
Stir.
(3)Postvaccinal queen bee tire homogenate is poured in closed round, in 34 DEG C of environment bottom fermentation 20h.So
After adjust the temperature to 37 DEG C, carry out anaerobic fermentation 18h, obtain the prebiotic oral liquid finished product of animal health queen bee tire.
Embodiment 2
Present embodiments provide application of the animal health of present invention announcement with queen bee tire probio oral liquid on piglet feed
1 materials and methods
1.1 test material
Queen bee tire probio oral liquid, main component queen bee fetoprotein zymotic fluid, containing animal bifidobacteria, lactoenterococcus and withered
Careless bacillus, total viable count 5.0 × 1011 CFU/L。
Experimental animal:The healthy tri-crossbreeding of body weight about 5.0kg 44, integrally sees that pig is in a good state of health.
1.2 experimental design
44 test pig parity are close, body weight is close, and the principle of male and female half and half is divided into 2 groups, per group of 3 repetitions, often repeatedly 6 ~
8.Control group fed basal diet(Original formula);Test group(Queen bee tire probio oral liquid group)Feed in addition and drink 100mL
/ kg body weight queen bee tire probio oral liquids.Experimental design scheme is shown in Table 1.
1.3 test daily rations
Test daily ration is groove feed for pigling, feed composition and trophic level(Slightly).
1.4 feeding and management
Test is carried out altogether 10 days.On-test is directly entered just tries the phase, does not do transition, feeds testing material.Conventinal breeding is managed,
Free choice feeding and drinking-water, immune disinfectant program is carried out according to pig farm conventional method.During test, observation each group swinery feeding daily
And health condition, accurate recording feed intake and diarrhoea situation.During off-test, weigh on an empty stomach by repetition.Calculating average daily gain,
Equal day feeding, feed-weight ratio and diarrhea rate.
1.5 data processing
Data processing and statistical analysis are carried out using SPSS statistical softwares, result of the test is represented with mean+SD, significantly
Property inspection adopt duncan's new multiple range method.
2 test results and analysis
Queen bee tire probio oral liquid is shown in Table 2 to the impact result of the test of Production Performance of Weaning Pigs and diarrhea rate
From table 2:Control group is compared, and queen bee tire probio oral liquid group feed-weight ratio and money meat ratio reduce respectively 15%(P<
0.01) with 9.11% (P<0.01), diarrhea rate significantly reduces 41.78% (P<0.01), average daily gain raises 19.2% (P<
0.05)。
3. conclusion
This result of the test shows, with queen bee tire probio oral liquid control group is substituted, and significantly improves the average daily gain of piglet,
Feed-weight ratio and money meat ratio are significantly reduced, and grice diarrhoea is substantially reduced.
Finally it should be noted that:Above specific description of embodiments of the present invention is not intended to limit the present invention, the technology of this area
Personnel carry out various modifications and improvements under the correlated condition of the present invention, should be included within the scope of the present invention.
Claims (5)
1. a kind of animal health queen bee tire probio oral liquid, is characterized in that, its preparation process is as follows:
1), in queen bee tire homogenate add lactose, pasteurization is carried out after mixing;
2), per milliliter of pretreated queen bee tire homogenate add animal probio based on total plate count 105~107 CFU;
3), carry out aerobic fermentation, round sealed thermal insulating, this stage does not ventilate in addition, and Bacillus utilize and consume fermentation
Oxygen in container, is next stage to cause anaerobic environment.
2. 30 ~ 35 DEG C of fermentation temperature, 16 ~ 24h of fermentation time;
4), carry out anaerobic fermentation, lactic acid bacteria has been consumed in the round of oxygen and has carried out anaerobic fermentation in early stage.
3. 35 ~ 37 DEG C of fermentation temperature, 18 ~ 20h of fermentation time.
4. animal health according to claim 1 queen bee tire probio oral liquid, is characterized in that described animal is prebiotic
Bacterium bag includes the bacillus licheniformis and bacillus subtilis of bacillus category, and enterococcus faecalis, the lactic acid intestines ball of lactic acid bacteria class
Bacterium, lactobacillus acidophilus, Lactobacillus plantarum, animal bifidobacteria, fermented bacterium is above-mentioned bacillus and lactic acid bacteria in use
Combination, i.e., at least include a kind of bacillus and a kind of lactic acid bacteria in fermented bacterium.
5. animal health according to claim 1 queen bee tire probio oral liquid, is characterized in that described pretreated
Queen bee tire homogenate, pretreatment mode is to add lactose in queen bee tire homogenate, and pasteurization, wherein lactose are carried out after mixing
Addition is in mass ratio:Queen bee tire homogenate:Lactose=100:1~100:2;Pasteurisation parameter is 55-60 DEG C, time 25-
30 minutes.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611205874.6A CN106615629A (en) | 2016-12-23 | 2016-12-23 | Bee larva probiotic oral liquid for animal healthcare |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611205874.6A CN106615629A (en) | 2016-12-23 | 2016-12-23 | Bee larva probiotic oral liquid for animal healthcare |
Publications (1)
Publication Number | Publication Date |
---|---|
CN106615629A true CN106615629A (en) | 2017-05-10 |
Family
ID=58828146
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201611205874.6A Pending CN106615629A (en) | 2016-12-23 | 2016-12-23 | Bee larva probiotic oral liquid for animal healthcare |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106615629A (en) |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2001321094A (en) * | 2000-05-12 | 2001-11-20 | Kenji Tokunaga | Method for producing lactic bacterium-containing royal jelly tablet |
US20060251633A1 (en) * | 2002-02-28 | 2006-11-09 | Salvadori Bruna B | Dietetic and/or pharmaceutical compositions for human and/or animal use based on probiotic microbial preparations |
CN103598654A (en) * | 2013-11-19 | 2014-02-26 | 山东省农业科学院农产品研究所 | Pollen active probiotic drink and preparation method thereof |
CN103622026A (en) * | 2013-12-18 | 2014-03-12 | 青岛根源生物技术集团有限公司 | Nutritional oral solution containing probiotics and preparation method thereof |
CN104472887A (en) * | 2014-12-29 | 2015-04-01 | 邳州市小河科技发展有限公司 | Probiotic feed additive |
CN105076413A (en) * | 2015-07-28 | 2015-11-25 | 天津中天精科科技有限公司 | Fruit and vegetable tablet containing lactic acid bacteria and preparation method of fruit and vegetable tablet |
CN105707439A (en) * | 2016-03-17 | 2016-06-29 | 中国科学院合肥物质科学研究院 | Preparation method of animal health-care nutrient solution rich in probiotics |
-
2016
- 2016-12-23 CN CN201611205874.6A patent/CN106615629A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2001321094A (en) * | 2000-05-12 | 2001-11-20 | Kenji Tokunaga | Method for producing lactic bacterium-containing royal jelly tablet |
US20060251633A1 (en) * | 2002-02-28 | 2006-11-09 | Salvadori Bruna B | Dietetic and/or pharmaceutical compositions for human and/or animal use based on probiotic microbial preparations |
CN103598654A (en) * | 2013-11-19 | 2014-02-26 | 山东省农业科学院农产品研究所 | Pollen active probiotic drink and preparation method thereof |
CN103622026A (en) * | 2013-12-18 | 2014-03-12 | 青岛根源生物技术集团有限公司 | Nutritional oral solution containing probiotics and preparation method thereof |
CN104472887A (en) * | 2014-12-29 | 2015-04-01 | 邳州市小河科技发展有限公司 | Probiotic feed additive |
CN105076413A (en) * | 2015-07-28 | 2015-11-25 | 天津中天精科科技有限公司 | Fruit and vegetable tablet containing lactic acid bacteria and preparation method of fruit and vegetable tablet |
CN105707439A (en) * | 2016-03-17 | 2016-06-29 | 中国科学院合肥物质科学研究院 | Preparation method of animal health-care nutrient solution rich in probiotics |
Non-Patent Citations (1)
Title |
---|
米春桃: ""发酵饲料对育肥猪屠宰后肉品质"", 《猪营养与饲料科技》 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106834163A (en) | Feed fermentation agent, fermented feed and preparation method thereof | |
Missotten et al. | Fermented liquid feed for pigs | |
CN1281147C (en) | Microbial fodder additive and its preparing method and use | |
CN102228129B (en) | Fermented feed | |
CN103798553B (en) | Environment-friendly pig feed additive and preparation method thereof | |
CN105851492A (en) | Traditional Chinese medicine probiotic fermented feed and preparation method | |
CN105475624A (en) | Microbial fermentation feed as well as production method and application thereof | |
CN110604226A (en) | Diarrhea-preventing growth-promoting composite feed additive for weaned piglets and preparation method thereof | |
WO2011115306A1 (en) | Feed-use-efficiency improver for ruminant animals | |
CN101268804B (en) | Microbial feed additive, preparation method and application thereof | |
CN108813123A (en) | It is a kind of improve intestine of young pigs health enteral nutrition packet and application | |
KR20210043877A (en) | Method for preparing fermented total mixed ration using microbial strain complex | |
CN104012803A (en) | Preparation method of fermented feed capable of preventing cow mastitis | |
CN102373162A (en) | Lactobacillus salivarius M6 and antibacterial composition containing same | |
CN105360615A (en) | Fermented feed raw material preparation as well as preparation method and application thereof | |
CN110506862A (en) | A kind of solid state fermentation feed and its preparation method and application improving weanling pig intestinal health and growth performance | |
CN102630844A (en) | Preparation method of biological feed for ruminants | |
Kore et al. | Effect of feeding traditionally prepared fermented milk dahi (curd) as a probiotics on nutritional status, hindgut health and haematology in dogs | |
CN106578354A (en) | Piglet compound premix, preparation method and daily ration for piglet | |
CN109198209A (en) | A kind of production method and its application of fermented maize | |
CN105285399A (en) | Liquid state composite microecological preparation for pigling and preparation method thereof | |
KR101709248B1 (en) | Probiotics for feed additives using a palm oil mesocarp, method for preparing, and utilizing the same | |
CN107319173A (en) | A kind of utilization marc mycoprotein promotes the feed of young goose growth | |
CN104738311B (en) | A kind of fish slurry fermented bean dregs and its production method and application | |
CN106819625A (en) | A kind of mandarin fish fermented feed and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170510 |
|
WD01 | Invention patent application deemed withdrawn after publication |