CN106610522A - Three-dimensional microscopic imaging device and method - Google Patents
Three-dimensional microscopic imaging device and method Download PDFInfo
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- CN106610522A CN106610522A CN201510705023.7A CN201510705023A CN106610522A CN 106610522 A CN106610522 A CN 106610522A CN 201510705023 A CN201510705023 A CN 201510705023A CN 106610522 A CN106610522 A CN 106610522A
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/362—Mechanical details, e.g. mountings for the camera or image sensor, housings
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Abstract
The invention discloses a three-dimensional microscopic imaging device and method. The device sequentially comprises an LED array, a sample carrying table, an objective lens, a red-blue color filter, a lens and a camera from the bottom to the top, and also comprises a computer and a red-blue stereo glasses. The center of the LED array is located on the central axis of the objective lens and the lens. The LED array and the camera are respectively connected with the computer. The method comprises the steps: placing a to-be-imaged sample on the sample carrying table; enabling the computer to transmit a control signal to the LED array, and enabling the LED array to generate white circular transparent light, wherein the light passes through the sample carrying table to be collected by the objective lens, and then is amplified for imaging, and finally enters the lens through the red-blue color filter; enabling the computer to drive the lens to carry out the sampling of a sample image passing through the lens, and enabling the camera to input the collected sample image into the computer through a data line for display; and carrying out the three-dimensional microscopic observation of the sample image displayed by the computer through the red-blue stereo glasses. The device is simple in structure, is low in cost, is good in effect, and can achieve the clear observation of the three-dimensional structure of a sample.
Description
Technical field
The invention belongs to optical microphotograph technical field of imaging, particularly a kind of stereo micrography imaging device and method.
Background introduction
Microscope is already penetrated in all trades and professions of ours at one's side as a kind of general observation, metrical instrument, its application,
It is today's society " materialization judge ", is one " exploring the key of microcosmoss " again.As the mankind observe material generation
Boundary is strided forward towards micron, nanoscale, promotes sending out for stereo micrography imaging technology to the needs of small items three-dimensional observation
Exhibition.Stereoscopic microscope can be described as stereomicroscope or referred to as anatomic microscope again, be widely used for biology, chemistry, thing
Reason, it is metallurgical, brewage, the various scientific research activities such as medical science, have great impact to the development and progress of the mankind.
Existing conventional stereo microscope, its core thinking are that the binocular parallax for simulating human eye forms three-dimensional imaging.Human perception
Nature depth is that have slight difference to cause due to images of left and right eyes viewing real world, the eyes of people have 4～6cm away from
From so image when actually we have seen that object in two eyes is differentiated, left eye sees object left surface
Composition is more, and right eye sees that the composition of object right flank is more.The two are as can just distinguish object after brain is comprehensive
Before and after, it is far and near, so as to produce stereoscopic vision, it is seen that be the image for having the depth of field.People are stood to the three-dimensional of object
Body vision is produced by visual disparity, so all can be such that Optical devices or structure that human eye produces visual disparity just produce
Raw three-dimensional stereoscopic visual.Conventional stereo microscope by a shared primary object lens to image objects after, through two groups of multiplying powers
The middle objective lens that can adjust separate, and then through the eyepiece group imaging of 12 degree～15 degree of angle, such words just can be with
There is provided angle different images respectively for right and left eyes, after the integrated treatment of brain, seen by person is exactly that a width has scape
Deep 3 D visual image.
Traditional stereoscopic microscope advantage is apparent：It is easy to use, it is easy to operate.Cannot but the problem that it is present is also
Make up：The angle of the cylinder mirror of (1) two eyepiece is 12 degree～15 degree, stereoscopic angle after such microscope batch production
It is certain.But due to human eye interpupillary distance individual variation in actual life, the stereoscopic angle of human eye is differed and is established a capital in this angle
In the range of, can so cause the undesirable of personal viewing effect；(2) dual pathways light path not only makes instrument heavy but also cost
Can also increase；(3) depth of focus can not be adjusted, observer cannot Autonomous Control institute observing samples observation thickness；(4) into
As second-rate, it is not possible to watch the details of object.
The content of the invention
It is an object of the invention to provide the good stereo micrography imaging device and method of a kind of simple structure, low cost, effect,
To realize clearly watching sample stereochemical structure.
The technical solution for realizing the object of the invention is：A kind of stereo micrography imaging device, including LED array, sample
Product object stage, object lens, red blue light filter, lens, camera, computer and red blue anaglyph spectacleses；Wherein LED array,
Sample stage, object lens, red blue light filter, lens, camera are set gradually from bottom to up, and the center of LED array
On object lens, the central axis of lens；LED array and camera are connected with computer；
Sample to be imaged is placed in into sample stage, computer sends control signal to LED array, makes LED array
The white circle illumination light that radius is R pixel is produced, the illumination light is collected by object lens through sample stage, object lens
The illumination light of collection being amplified after imaging and lens being incident to through red blue light filter, computer drives camera is to through thoroughly
The sample image of mirror is sampled, and the sample image of collection is shown by camera through data wire input computer, is passed through
Red blue anaglyph spectacleses carry out stereomicroscopy viewing to the sample image of Computer display.
Preferably, the LED array adopts P4 series LED arrays, P4 series LEDs array provide red, green,
Blueness, cyan, pink colour, yellow, the illumination light of seven kinds of colors of white light, the present invention adopt white illumination light；P4 is serial
The unit board size of LED array be 128mm*128mm, number of pixels be 32*32, pel spacing 4mm, each picture
Vegetarian refreshments individually can be lighted.
Preferably, the LED array is 75～85mm apart from the distance of sample stage upper surface.
Further, the red blue light filter is circular light transmission piece, and the optical filter is divided into two semicircles, and this two and half
Circle is respectively Red lightscreening plate and blue color filter；Red blue light filter is arranged on the back focal plane of object lens, by formed by object lens
As separating.
Further, the CCD camera lenses of the camera are located on the back focal plane of lens.
Further, the solid colour of the red blue anaglyph spectacleses and red blue light filter.
A kind of stereo micrography imaging method, step are as follows：
Sample to be imaged is placed in sample stage by step 1, and computer sends control signal to LED array, makes
LED array produces radius for the white circle illumination light of R, numbers of the wherein R for pixel；
Step 2, the illumination light that step 1 is produced are collected by object lens through sample stage, and the illumination light of collection is entered by object lens
Row is zoomed into and is incident to lens through red blue light filter as after；
Step 3, computer drives camera are sampled to the sample image through lens, the sample image that camera will be gathered
Shown through data wire input computer；
Step 4, carries out stereomicroscopy viewing to the sample image of Computer display by red blue anaglyph spectacleses, while passing through
Produced by computer regulated LED array, the radius R of white circle illumination light, changes illumination numerical aperture angle right to realize
The imaging of sample different-thickness layer.
Further, by the radius R of white circle illumination light produced by computer regulated LED array described in step 4,
Change illumination numerical aperture angle to realize the imaging to sample different-thickness layer, wherein illumination numerical aperture angle θNAAnd circle
Relation between the radius R of illumination light is as follows：
θNA=arctan R/H
Wherein, H is distance of the LED array apart from sample stage upper surface.
Compared with prior art, its remarkable advantage is the present invention：(1) it is red by adding on microscope objective back focal plane
Blue light filter, so as to replace the microscopical double light path of conventional stereo, simplifies system design, not only makes imaging system easy
And effective reduces cost；(2) by being used as lighting source using LED array, realize to the flexible of microscope depth of focus
It is adjustable；(3) by by collected by camera to image pass on computer display, such that it is able to realize tie three-dimensional to sample
The clear viewing of structure；(4) light source with system itself, the restricted reduction of observation condition less by external interference,
Can be watched under dark background.
Description of the drawings
Fig. 1 is the structural representation of stereo micrography imaging device of the present invention.
Fig. 2 is the white circle illumination light schematic diagram of LED array generation in the present invention.
Fig. 3 is the structural representation of red blue light filter in the present invention.
Fig. 4 is the sample drawing shown by embodiment of the present invention Computer.
Specific embodiment
Below in conjunction with the accompanying drawings and specific embodiment is described in further detail to the present invention.
With reference to Fig. 1, stereo micrography imaging device of the present invention, including LED array 1, sample stage 2, object lens 3,
Red blue light filter 4, lens 5, camera 6, computer 7 and red blue anaglyph spectacleses 8；Wherein LED array 1, sample is carried
Thing platform 2, object lens 3, red blue light filter 4, lens 5, camera 6 are set gradually from bottom to up, and LED array 1
It is centrally located on object lens 3, the central axis of lens 5；LED array 1 and camera 6 are connected with computer 7；
Sample to be imaged is placed in into sample stage 2, computer 7 sends control signal to LED array 1, makes LED
Array 1 produces the white circle illumination light that radius is R pixel, as shown in Fig. 2 the illumination light passes through sample loading
Platform 2 is collected by object lens 3, and object lens 3 are incident to through red blue light filter 4 after the illumination light of collection is amplified imaging
Mirror 5, computer 7 drive 6 pairs of sample images through lens 5 of camera to sample, the sample drawing that camera 6 will be gathered
As being shown through data wire input computer 7, sample image computer 7 shown by red blue anaglyph spectacleses 8
Carry out stereomicroscopy viewing.
Preferably, the LED array 1 adopts P4 series LED arrays, P4 series LEDs array to provide red, green
Color, blueness, cyan, pink colour, yellow, the illumination light of seven kinds of colors of white light, the present invention adopt white illumination light source；P4
The unit board size of series LED array be 128mm*128mm, number of pixels be 32*32, pel spacing 4mm,
Each pixel individually can be lighted.
Preferably, the LED array 1 is 75～85mm apart from the distance of 2 upper surface of sample stage.
Further, as shown in figure 3, the red blue light filter 4 is circular light transmission piece, the optical filter is divided into two
Semicircle, two semicircles are respectively Red lightscreening plate and blue color filter；Red blue light filter 4 is arranged at rear Jiao of object lens 3
On face, 3 imaging of object lens is separated.
Further, the CCD camera lenses of the camera 6 are located on the back focal plane of lens 5.
Further, the solid colour of the red blue anaglyph spectacleses 8 and red blue light filter 4.
Stereo micrography imaging method of the present invention, step are as follows：
Sample to be imaged is placed in sample stage 2 by step 1, and computer 7 sends control signal to LED array 1,
LED array 1 is made to produce the white circle illumination light that radius is R, numbers of the wherein R for pixel；
Step 2, the illumination light that step 1 is produced are collected by object lens 3 through sample stage 2, the photograph that object lens 3 will be collected
Mingguang City is incident to lens 5 through red blue light filter 4 after being amplified imaging；
Step 3, computer 7 drive 6 pairs, camera to sample through the sample images of lens 5, and camera 6 will be gathered
Sample image is shown through data wire input computer 7；
Step 4, carries out stereomicroscopy viewing to the sample image that computer 7 shows by red blue anaglyph spectacleses 8, while
The radius R of circular illumination light produced by LED array 1 is adjusted by computer 7, changes illumination numerical aperture angle with reality
The now imaging to sample different-thickness layer, wherein illumination numerical aperture angle θNAAnd the pass between the radius R of circular illumination light
System is as follows：
θNA=arctan R/H
Wherein, H is distance of the LED array 1 apart from 2 upper surface of sample stage.
Embodiment 1
The step of invention device being discussed in detail below in conjunction with the accompanying drawings and realizes to sample stereos copic viewing.
(1) combine accompanying drawing and the invention device is discussed in detail：
With reference to Fig. 1, the stereo micrography imaging device of the present invention, including P4 series LEDs array 1, sample stage 2,
Object lens 3, red blue light filter 4, lens 5, camera 6, computer 7 and red blue anaglyph spectacleses 8.Wherein P4 series LEDs
Array 1 is installed on below sample stage 2, away from 2 upper surface of sample stage about 80mm or so and which is centrally located at thing
On the central axis of mirror 3 and lens 5.The control P4 series LEDs of computer 7 array 1 produces required radius R (these
In radius R refer to pixel number) white illumination light, the white illumination light transmission sample stage 2 of generation is by object lens
3 collect, then by red 4 light splitting of blue light filter after processing and amplifying imaging, then the further imaging processing of Jing lens 5,
Under the control of computer 7, drive camera 6 to adopt figure.Computer 7 is connected with P4 series LEDs array 1 and camera 6,
Realize controlling which by software system.
P4 series LEDs array 1, can provide redness, green, blueness, cyan, pink colour, yellow, seven kinds of white light
The illumination light of color.P4 series LEDs array 1 (unit board size 128mm*128mm, number of pixels 32*32, as
Plain spacing 4mm) it is placed directly below sample stage 2, which is apart from object stage upper surface apart from H typically in 80mm
Left and right, and the center of P4 series LEDs array 1 is on the optical axis of microscope optical system.Each pixel
Individually light, the circular radius R that shows on P4 series LED cell boards, color controllable, central coordinate of circle are set in LED
The middle position of array, as shown in Figure 2.The radius R and color of lighting source, this enforcement are controlled by computer 7
Example has only used white light.Red blue optical filter 4 is put on the back focal plane of microscope objective, so just can realize to institute into
As carrying out light splitting, as shown in Figure 3.Lens 5 are tube lens, realize the further amplification and process to imaging.
Camera 6 gathers image under the control of computer 7, is displayed on the display screen of computer 7, and observer wears red blue vertical
Body glasses 8 can just realize the stereos copic viewing to sample.
(2) realize that the concrete steps of the three-dimensional body viewing presented to the device include：
Sample to be imaged is placed in sample stage 2 by step 1, and computer 7 sends control signal to LED array 1,
LED array 1 is made to produce the white circle illumination light that radius is R, numbers of the wherein R for pixel；
Step 2, the illumination light that step 1 is produced are collected by object lens 3 through sample stage 2, the photograph that object lens 3 will be collected
Mingguang City is incident to lens 5 through red blue light filter 4 after being amplified imaging；
Step 3, computer 7 drive 6 pairs, camera to sample through the sample images of lens 5, and camera 6 will be gathered
Sample image is shown through data wire input computer 7；
Step 4, carries out stereomicroscopy viewing to the sample image that computer 7 shows by red blue anaglyph spectacleses 8, while
The radius R of circular illumination light produced by LED array 1 is adjusted by computer 7, changes illumination numerical aperture angle with reality
The now imaging to sample different-thickness layer, wherein illumination numerical aperture angle θNAAnd the pass between the radius R of circular illumination light
System is as follows：
θNA=arctan R/H
Wherein, H is distance of the LED array 1 apart from 2 upper surface of sample stage, and the present embodiment takes 80mm.
Fig. 4 is the display image of the diffraction optical element sample photographed using this device, including in Computer display
On device, and wear red blue anaglyph spectacleses clearly axonometric chart can be observed.
To sum up, it is of the invention by red blue light filter is added on microscope objective back focal plane, so as to replace conventional stereo micro-
The double light path of mirror, simplifies system design, reduces cost, improves image quality；By (single using LED array
First board size 128mm*128mm, number of pixels 32*32, pel spacing 4mm) as lighting source, realize to aobvious
Flexible adjustable, the system automatic light source of micro mirror depth of focus itself is less by external interference；By the image for arriving collected by camera
Pass to display screen and can realize the clear viewing to sample stereochemical structure.
Claims (8)
1. a kind of stereo micrography imaging device, it is characterised in that including LED array (1), sample stage (2),
Object lens (3), red blue light filter (4), lens (5), camera (6), computer (7) and red blue anaglyph spectacleses (8)；
Wherein LED array (1), sample stage (2), object lens (3), red blue light filter (4), lens (5), camera
(6) set gradually from bottom to up, and LED array (1) is centrally located at object lens (3), the central shaft of lens (5)
On line；LED array (1) and camera (6) are connected with computer (7)；
Sample to be imaged is placed in into sample stage (2), computer (7) sends control letter to LED array (1)
Number, make LED array (1) produce the white circle illumination light that radius is R pixel, the illumination light is carried through sample
Thing platform (2) is collected by object lens (3), and object lens (3) are amplified after imaging the illumination light of collection through red blue colour filter
Piece (4) is incident to lens (5), and computer (7) drives camera (6) to carry out the sample image through lens (5)
The sample image for gathering is shown by sampling, camera (6) through data wire input computer (7), by red blue vertical
Body glasses (8) carry out stereomicroscopy viewing to the sample image that computer (7) shows.
2. stereo micrography imaging device according to claim 1, it is characterised in that the LED array (1)
Using P4 series LED arrays, P4 series LEDs array provide redness, green, blueness, cyan, pink colour, yellow,
The illumination light of seven kinds of colors of white light, the present invention adopt white illumination light；The unit board size of P4 series LED arrays is
128mm*128mm, number of pixels are 32*32, pel spacing 4mm, and each pixel individually can light.
3. stereo micrography imaging device according to claim 1, it is characterised in that the LED array (1)
It is 75～85mm apart from the distance of sample stage (2) upper surface.
4. stereo micrography imaging device according to claim 1, it is characterised in that the red blue light filter (4)
For circular light transmission piece, the optical filter is divided into two semicircles, and two semicircles are respectively Red lightscreening plate and blue filter
Piece；Red blue light filter (4) is arranged on the back focal plane of object lens (3), will be object lens (3) imaging separate.
5. stereo micrography imaging device according to claim 1, it is characterised in that the CCD of the camera (6)
Camera lens is located on the back focal plane of lens (5).
6. stereo micrography imaging device according to claim 1, it is characterised in that the red blue anaglyph spectacleses (8)
With the solid colour of red blue light filter (4).
7. a kind of stereo micrography imaging method based on stereo micrography imaging device described in claim 1, it is characterised in that
Step is as follows：
Sample to be imaged is placed in sample stage (2) by step 1, and computer (7) is sent to LED array (1)
Control signal, makes LED array (1) produce the white circle illumination light that radius is R, numbers of the wherein R for pixel；
Step 2, the illumination light that step 1 is produced are collected by object lens (3) through sample stage (2), and object lens (3) will
The illumination light of collection is incident to lens (5) through red blue light filter (4) after being amplified imaging；
Step 3, computer (7) drive camera (6) to sample the sample image through lens (5), camera (6)
The sample image of collection is shown through data wire input computer (7)；
Step 4, carries out stereomicroscopy viewing to the sample image that computer (7) shows by red blue anaglyph spectacleses (8),
Adjust the radius R of circular illumination light produced by LED array (1) simultaneously by computer (7), change illumination numerical
Angular aperture is realizing the imaging to sample different-thickness layer.
8. stereo micrography imaging method according to claim 7, it is characterised in that by calculating described in step 4
Machine (7) adjusts the radius R of white circle illumination light produced by LED array (1), change illumination numerical aperture angle with
Realize the imaging to sample different-thickness layer, wherein illumination numerical aperture angle θNAAnd the radius R of circular illumination light between
Relation is as follows：
θNA=arctan R/H
Wherein, H is distance of the LED array (1) apart from sample stage (2) upper surface.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510705023.7A CN106610522A (en) | 2015-10-26 | 2015-10-26 | Three-dimensional microscopic imaging device and method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510705023.7A CN106610522A (en) | 2015-10-26 | 2015-10-26 | Three-dimensional microscopic imaging device and method |
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| CN201510705023.7A Pending CN106610522A (en) | 2015-10-26 | 2015-10-26 | Three-dimensional microscopic imaging device and method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109167891A (en) * | 2018-08-23 | 2019-01-08 | 谢明仿 | A kind of touch screen type has the image processing apparatus of support construction |
| CN109270670A (en) * | 2018-10-31 | 2019-01-25 | 上海理鑫光学科技有限公司 | LED array light source, without lens microscope and image processing method |
| CN110554495A (en) * | 2018-05-30 | 2019-12-10 | 香港理工大学 | Light field optical microscope and light field microscopic imaging analysis system thereof |
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| CN101528113A (en) * | 2006-08-30 | 2009-09-09 | 威神斯扣普技术有限公司 | Portable imaging system employing a miniature endoscope |
| CN102823230A (en) * | 2011-01-17 | 2012-12-12 | 松下电器产业株式会社 | Imaging device |
| CN104765138A (en) * | 2015-04-17 | 2015-07-08 | 南京理工大学 | Multi-mode micro-imaging system and method based on LED array |
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| CN1202239A (en) * | 1995-11-14 | 1998-12-16 | 摩西·拉宗 | Computer stereo vision system and method |
| CN101528113A (en) * | 2006-08-30 | 2009-09-09 | 威神斯扣普技术有限公司 | Portable imaging system employing a miniature endoscope |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110554495A (en) * | 2018-05-30 | 2019-12-10 | 香港理工大学 | Light field optical microscope and light field microscopic imaging analysis system thereof |
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| CN109167891A (en) * | 2018-08-23 | 2019-01-08 | 谢明仿 | A kind of touch screen type has the image processing apparatus of support construction |
| CN109270670A (en) * | 2018-10-31 | 2019-01-25 | 上海理鑫光学科技有限公司 | LED array light source, without lens microscope and image processing method |
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Application publication date: 20170503 |