CN106609224B - Preparation method of cordyceps militaris wine - Google Patents
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Abstract
The invention relates to a preparation method of cordyceps militaris wine, which is characterized by comprising the following steps: step 1, in the solid culture stage of cordyceps militaris, separating part of solid culture medium and the sporophores growing in the solid culture medium from the whole culture medium according to the shape of a container; step 2, placing the separated culture medium and the sporophore bundles growing in the culture medium into a container; step 3, fixing the solid culture medium at the bottom of the container by using a support; step 4, injecting wine into the container; and step 5, fixing for a certain time, and taking out the support; wherein the solid culture medium is a culture medium containing grains as main components. The cordyceps militaris wine prepared by the method has high nutritive value and unique artistic aesthetic feeling.
Description
Technical Field
The invention relates to a preparation method of cordyceps militaris wine, in particular to a method for producing cordyceps militaris wine growing in a wine jar by using a specific grain culture medium, and belongs to the field of medical food.
Background
Cordyceps militaris (Cordyceps militaris) is a model species of Ascomycotina, Clavicipitales, Cordyceps, and has a scientific name of Cordyceps militaris (Vuill.) Fr. Modern science proves that the cordyceps militaris not only has special nutritional value, but also has obvious medicinal value. Wherein the extract mainly contains cordycepic acid, cordycepin, adenosine, and Cordyceps polysaccharide. Has effects in tonifying lung yin and kidney yang, and can be used for treating kidney deficiency, sexual impotence, spermatorrhea, soreness of waist and knees, and asthenia after illness. The traditional Chinese medicine considers that the traditional Chinese medicine has the effects of strengthening body resistance and consolidating constitution, has obvious curative effects on senile chronic bronchitis and pulmonary heart disease, can improve the detoxifying capability of the liver, has the effect of protecting the liver, and improves the antiviral and anti-radiation capability of the body. The traditional Chinese medicine considers that the cordyceps sinensis enters the lung and kidney two channels, can tonify lung yin and kidney yang, is mainly used for treating kidney deficiency, impotence and spermatorrhea, soreness and pain of waist and knees, weakness after illness, chronic cough and weakness, cough with phlegm and blood, spontaneous perspiration and night sweat and the like, and is the only traditional Chinese medicine capable of balancing and adjusting yin and yang simultaneously.
The cordyceps militaris soaked in the wine is a commonly adopted nutrition absorption method, and the soaking process is as follows: putting Cordyceps militaris (or soaking together with other medicinal materials such as Ginseng radix and fructus Lycii) in a container, adding Chinese liquor, sealing, and standing for a proper number of days for drinking. By adopting the brewing method, the cordyceps militaris is placed in a container by utilizing ready-cultured sporostalk bundles, scattered and some cordyceps militaris float on the surface layer of the wine, the active ingredients of the cordyceps militaris are not completely soaked in the wine, the good growth situation of the cordyceps militaris in the wine is not shown, and the visual aesthetic feeling of the cordyceps militaris is not highlighted. Does not present the characteristics of the ancient wine culture in China.
Disclosure of Invention
The invention aims to provide a preparation method of cordyceps militaris wine, which not only has higher nutritive value, but also has unique artistic aesthetic feeling.
The purpose of the invention is realized by the following technical scheme:
a preparation method of cordyceps militaris wine comprises the following steps:
step 1, in the solid culture stage of cordyceps militaris, separating part of solid culture medium and the sporophores growing in the solid culture medium from the whole culture medium according to the shape of a container;
step 2, placing the separated culture medium and the sporophore bundles growing in the culture medium into a container;
step 3, fixing the solid culture medium at the bottom of the container by using a support;
step 4, injecting wine into the container; and
wherein the solid culture medium is a culture medium containing grains as main components.
Further, the grain is selected from one or more of wheat, corn, rice, millet, soybean meal, buckwheat, barley, oat, brown rice and polished round-grained rice.
Further, the coremium harvested in step 1 is a coremium grown to maturity.
Further, the area of the solid culture medium obtained in the step 1 can be slightly smaller than the area required by the solid culture medium which can be put into a container, the shape is not limited, and the specific shape is based on the principle of beauty.
Further, step 2, placing the solid culture medium in front of a wine container, and thinning the culture medium to ensure that the sporophore bundles growing in the culture medium are not scattered; further, thinning the culture medium to a thickness of less than 2 cm; further, thinning the culture medium to a thickness of less than 1 cm; further, the medium is thinned to a thickness of less than 0.5 cm.
Further, the support in step 3 is a cylindrical structure made of wood, glass, stainless steel or any other material which does not react with the base wine, such as a wood rod, a glass rod, a stainless steel rod and the like; the height of the container is slightly less than the height from the bottom of the container to the opening of the container.
Further, the height of the wine injection in the step 4 is higher than that of the coremium; furthermore, the height of the wine injection is more than 1cm higher than the sporophores.
Further, the fixed time of step 5 is within 45 days; further, the fixed time is within 35 days; further, the fixation time is within 30 days.
Further, taking out the support in the step 5 and continuing to soak for more than 60 days; further, continuously soaking for more than 80 days; further, the soaking was continued for 100 days.
The culture process of the cordyceps militaris provided by the invention comprises the following steps: performing liquid culture on cordyceps militaris strains for amplification; and performing solid culture on the amplified strains. The liquid culture and solid culture methods can refer to the culture method of Cordyceps militaris disclosed in the prior art. The liquid culture aims at strain amplification, and can adopt a conventional strain amplification culture method, including any one or combination of several methods of slant culture, shake culture, seeding tank culture and fermentation tank culture; the culture medium is conventional liquid culture medium in the art, such as PSA culture medium or yeast extract powder-compound amino acid-sucrose culture medium, yeast extract powder-white sugar-soybean protein hydrolysate culture medium, bran decoction-white sugar culture medium, etc.; further preferably a PSA culture medium or a yeast extract powder-compound amino acid-sucrose culture medium; furthermore, the PSA culture medium comprises the following components in proportion: 15-25% of potato, 1-5% of cane sugar and 1-5% of agar; the yeast extract powder-compound amino acid-sucrose culture medium comprises the following components in proportion: 0.5-2% of yeast extract powder, 0.2-1% of compound amino acid and 2-5% of cane sugar. Furthermore, the PSA culture medium comprises the following components in proportion: 20% of potato, 2% of cane sugar and 2% of agar; the yeast extract powder-compound amino acid-sucrose culture medium comprises the following components in proportion: 1% of yeast extract powder, 0.5% of compound amino acid and 3.5% of cane sugar. In the solid culture process, shading culture is adopted in the growth stage of the mycelium, the culture temperature is 22-24 ℃, and the relative humidity is 65-70%; the cultivation is carried out by illumination from the beginning of the coremium to the harvesting stage, the cultivation temperature is 20-22 ℃, the relative humidity is 65-70%, and the illumination intensity is ensured to be 100-200Lx when the coremium is started.
The Cordyceps militaris (cordyces militaris (Vuill.) Fr.) used in the present invention is a broad-spread species. The Cordyceps militaris is distributed in provinces such as Hubei, Hebei, Jilin, Shaanxi, Yunnan, Guangxi, Guizhou, Sichuan and Taiwan. The strain can be obtained according to the collection method of common Chinese medicinal materials, is a strain known in the prior art, and can be purchased from commercial approaches (such as edible fungus factories and research institutes).
The invention has the beneficial effects that:
① the invention puts the sporophores bunch and solid culture medium into the wine jar, it is supported by the support, it prevents the sporophores bunch from floating, after the month, after the osmotic pressure of sporophores bunch and wine is balanced, the support is removed, the sporophores bunch and culture medium will be stable at the bottom of the bottle, it will not float, it keeps beautiful situation, ② the content of nutrition component is high, the invention cordyceps militaris wine is cultivated for about 100 days, its adenosine content reaches 28.3 mug/ml, 1.77 times of the long-scale infusion method, HEA (N6- (2-hydroxyethyl adenosine, also called callosone, is the peculiar component of cordyceps sinensis, has been regarded as one of the quality control index of cordyceps products) content reaches 54.12 mug/ml, 1.87 times of cordyceps militaris wine infused by the conventional method.
Drawings
FIG. 1 tool for cutting solid media
FIG. 2 shows Cordyceps militaris wine prepared by the method of the invention
FIG. 3 is a graph showing the relationship between adenosine content in Cordyceps militaris wine and time
FIG. 4 is a graph showing the relationship between HEA content and time in Cordyceps militaris wine
Experimental example 1 examination of solid Medium
1. Experimental methods
The Cordyceps militaris strain which is subjected to scale-up culture in example 1 is taken as a solid culture medium, the materials listed in Table 1 are respectively used for solid culture according to the method of example 9, the cordyceps militaris strain is harvested and fixed according to the method of example 10 (the experiment number of each culture medium is 20 bottles), the support is removed after 45 days, and the states of each group of the sporophores and the culture medium are observed.
2. Results of the experiment
The results are shown in Table 1.
TABLE 1 State of fixation of the 45 d-spore-peduncle on different types of solid culture media
The result shows that the grain culture medium can reach the balance within 45 days of fixation and does not float upwards any more; while other types of solid media are not stable within 45 days of fixation and are not suitable as solid media for the method of the present invention.
Detailed Description
EXAMPLE 1 liquid culture
Slant culture: inoculating the separated and purified strains into a slant test tube, and then putting the slant test tube inoculated with the strains into an incubator at 22 ℃, wherein the culture time is 7 days, and when hyphae grow over the test tube;
and (3) shake flask culture: placing 200m1 liquid culture medium into a 500m1 triangular flask, sterilizing for 30 minutes under 0.11Mpa, cooling to room temperature, inoculating strains of 1 inclined test tube to the culture medium of the 500m1 triangular flask, and culturing in a constant temperature shaking incubator at 22 +/-1 ℃ and 150 rpm for 3 days;
seed tank culture: charging 20L liquid culture medium into 50L airlift fermentation tank, wherein the temperature of the culture medium is higher than 95 deg.C, adding edible defoaming agent in an amount of 0.03 wt% of the culture medium, introducing steam under 0.11Mpa, heating to 121 deg.C, and maintaining the pressure for 30-45 min; after sterilization, when the mixture is cooled to 20 ℃, 4 bottles of the cultured 500m1 shake flask strains are inoculated into a culture medium, the culture temperature is 22 +/-1 ℃, and the ventilation volume is 1: 0.5V/V min, and maintaining the pressure of 4.903-7.0845 × 104Pa, culturing for 3 days in an aerated manner to achieve the aim ofAfter several growth periods, the final culture volume was 20L.
Culturing in a fermentation tank: charging 200L liquid culture medium into 500L airlift fermentation tank, wherein the temperature of the culture medium is higher than 95 deg.C, adding edible defoaming agent in an amount of 0.03 wt% of the culture medium, introducing steam under 0.11Mpa, heating to 121 deg.C, and maintaining the pressure for 30-45 min; after sterilization, when the seed is cooled to 20 ℃, the seed in the 200L seed tank cultured as above is inoculated and cultured, the culture temperature is 22 +/-1 ℃, and the ventilation volume is 1: 0.5V/V min, and maintaining the pressure of 4.903-7.0845 × 104Pa, after 3 days aeration culture, the logarithmic growth phase is reached. The final culture volume was 200L.
Slant tube medium: every 1 liter of liquid contains 200 g of potato decoction, 20 g of cane sugar, 20 g of agar and the balance of water to 1000 ml, and the pH value is 6.5;
liquid culture medium in shake flasks, seed tanks and fermentors: contains yeast extract powder 1%, compound amino acid 0.5%, white sugar 3.5%, and water to 100% and has pH of 6.5.
Example 2 liquid culture
Slant culture: inoculating the separated and purified strains into a slant test tube, and then putting the slant test tube inoculated with the strains into an incubator at 22 ℃, wherein the culture time is 7 days, and when hyphae grow over the test tube;
and (3) shake flask culture: placing 200m1 liquid culture medium into a 500m1 triangular flask, sterilizing for 30 minutes under 0.11Mpa, cooling to room temperature, inoculating strains of 1 inclined test tube to the culture medium of the 500m1 triangular flask, and culturing in a constant temperature shaking incubator at 22 +/-1 ℃ and 150 rpm for 3 days;
seed tank culture: charging 20L liquid culture medium into 50L airlift fermentation tank, wherein the temperature of the culture medium is higher than 95 deg.C, adding edible defoaming agent in an amount of 0.03 wt% of the culture medium, introducing steam under 0.11Mpa, heating to 121 deg.C, and maintaining the pressure for 30-45 min; after sterilization, when the mixture is cooled to 20 ℃, 4 bottles of the cultured 500m1 shake flask strains are inoculated into a culture medium, the culture temperature is 22 +/-1 ℃, and the ventilation volume is 1: 0.5V/V min, and maintaining the pressure of 4.903-7.0845 × 104Pa, 3 days aeration culture to achieve logarithmic growthAfter this time, the final culture volume was 20L.
Slant tube medium: every 1 liter of liquid contains 200 g of potato decoction, 20 g of cane sugar, 20 g of agar and the balance of water to 1000 ml, and the pH value is 6.5;
shake flask and seed tank culture medium: each 1 liter of liquid contains 30 g of yeast extract powder, 30 g of white granulated sugar and 5 g of soybean protein hydrolysate, and water is added to supplement the volume to 1000 ml, and the pH value is 6.5.
EXAMPLE 3 liquid culture
Slant culture: inoculating the separated and purified strains into a slant test tube, and then putting the slant test tube inoculated with the strains into an incubator at 22 ℃, wherein the culture time is 7 days, and when hyphae grow over the test tube;
seed tank culture: filling 20L of liquid culture medium into a 50L airlift seeding tank, wherein the temperature of the culture medium is higher than 95 ℃, adding an edible antifoaming agent, the adding amount is 0.03 percent of the weight of the culture medium, introducing steam under the pressure of 0.11Mpa, heating to 121 ℃, and maintaining the pressure for 30-45 minutes; after sterilization, when the test tube is cooled to 20 ℃, 4 bottles of strains in the slant test tube are inoculated into a culture medium, the culture temperature is 22 +/-1 ℃, and the ventilation volume is 1: 0.5V/V min, and maintaining the pressure of 4.903-7.0845 × 104Pa, after 3 days aeration culture, the culture volume is 20L after reaching the logarithmic growth phase.
Culturing in a fermentation tank: charging 200L liquid culture medium into 500L airlift fermentation tank, wherein the temperature of the culture medium is higher than 95 deg.C, adding edible defoaming agent in an amount of 0.03 wt% of the culture medium, introducing steam under 0.11Mpa, heating to 121 deg.C, and maintaining the pressure for 30-45 min; after sterilization, when the seed is cooled to 20 ℃, the seed in the 200L seed tank cultured as above is inoculated and cultured, the culture temperature is 22 +/-1 ℃, and the ventilation volume is 1: 0.5V/V min, and maintaining the pressure of 4.903-7.0845 × 104Pa, after 3 days aeration culture, the logarithmic growth phase is reached. The final culture volume was 200L.
Slant tube medium: every 1 liter of liquid contains 200 g of potato decoction, 20 g of cane sugar, 20 g of agar and the balance of water to 1000 ml, and the pH value is 6.5;
seeding tank and fermentation tank culture medium: every 1L of the liquid contains 40 g of bran cooking juice, 30 g of white granulated sugar and the balance of supplementary water to 1000 ml, and the pH value is 6.5.
Example 4 solid culture
Inoculation: irradiating the culture container with ultraviolet light in a superclean bench or a hundred-level laminar flow hood (below) for 0.5h before inoculation; the strain obtained by the scale-up culture in examples 1 to 3 was inoculated into the solid medium of any one of examples 4 to 9 in an amount of 7% per culture vessel, and the inoculated vessel was placed in a culture chamber for culture.
The culture conditions are as follows: the temperature of the culture room is 22-24 ℃, and the relative humidity of air is 65-70%; first, the cultivation room is shielded from light, and the spawn running room is basically dark. And (5) when the mycelium grows over the feed surface (indoor culture for 3-5 days), switching to illumination culture. Periodically checking after inoculation, and timely removing culture containers with growth potential differences and infected infectious microbes. Then, in the stage of inducing the formation of the coremium, the temperature of the culture room is kept at 20-22 ℃, the relative air humidity is 65-70%, and the scattered light (100 Lx-200 Lx) is ensured to induce the formation of the coremium. The culture room needs ventilation at the right time to keep the air of the spawn running room fresh.
Example 5 solid Medium
After wheat is cleaned and water is controlled, a proper amount of water is added, and the weight ratio of the wheat to the water is 1: 1.4, mixing uniformly; placing into a sealed polypropylene box (a hole is formed in the upper cover of the box, and a breathable film is stuck in the hole); placing the culture container filled with culture medium in a sterilizing pot, and sterilizing at 121 deg.C for 30-50 min; after sterilization, the culture vessel was transferred into a buffer chamber, naturally cooled to 24 ℃ or lower, and transferred into an inoculation chamber.
Example 6 solid Medium
Washing rice, controlling water, and adding a proper amount of water, wherein the weight ratio of the rice to the water is 1: 1.3, uniformly mixing; mixing, and placing into a sealed polypropylene box (with a hole in the upper cover and a gas-permeable membrane in the hole); placing the culture container filled with culture medium in a sterilizing pot, and sterilizing at 121 deg.C for 30-50 min; after sterilization, the culture vessel was transferred into a buffer chamber, naturally cooled to 24 ℃ or lower, and transferred into an inoculation chamber.
Example 7 solid Medium
After brown rice is cleaned and water is controlled, a proper amount of water is added, and the weight ratio of brown rice is as follows: 1 part of water: 1.5, mixing uniformly; mixing, and placing into a sealed polypropylene box (with a hole in the upper cover and a gas-permeable membrane in the hole); placing the culture container filled with culture medium in a sterilizing pot, and sterilizing at 121 deg.C for 30-50 min; after sterilization, the culture vessel was transferred into a buffer chamber, naturally cooled to 24 ℃ or lower, and transferred into an inoculation chamber.
Example 8 solid Medium
After millet is cleaned and water is controlled, a proper amount of water is added, wherein the weight ratio of the millet to the water is 1: 1.3, uniformly mixing; mixing, and placing into a sealed polypropylene box (with a hole in the upper cover and a gas-permeable membrane in the hole); placing the culture container filled with culture medium in a sterilizing pot, and sterilizing at 121 deg.C for 30-50 min; after sterilization, the culture vessel was transferred into a buffer chamber, naturally cooled to 24 ℃ or lower, and transferred into an inoculation chamber.
Example 9 solid Medium
69% of corncobs, 16% of cottonseed hulls, 11% of sawdust, 3% of gypsum, 1% of lime and solid materials: water 1: 1.5 (according to the water content of the raw materials), uniformly mixing, and then putting into a sealed polypropylene box (a hole is formed in the upper cover of the box, and a breathable film is stuck in the hole); placing the culture container filled with culture medium in a sterilizing pot, and sterilizing at 121 deg.C for 30-50 min; after sterilization, the culture vessel was transferred into a buffer chamber, naturally cooled to 24 ℃ or lower, and transferred into an inoculation chamber.
Example 10 solid Medium
Crop straw culture medium: 50% of corn straw powder, 20% of wheat straw powder, 20% of cotton straw, 5% of soybean meal, 5% of corn flour and solid materials: water 1: 1.5 (according to the water content of the raw materials), uniformly mixing, and then putting into a sealed polypropylene box (a hole is formed in the upper cover of the box, and a breathable film is stuck in the hole); placing the culture container filled with culture medium in a sterilizing pot, and sterilizing at 121 deg.C for 30-50 min; after sterilization, the culture vessel was transferred into a buffer chamber, naturally cooled to 24 ℃ or lower, and transferred into an inoculation chamber.
Example 11 solid Medium
Crop husk culture medium: 35% of wheat bran, 20% of rice hull powder, 35% of rapeseed hull powder, 5% of soybean meal, 5% of corn flour and a solid material: water 1: 1.5 (according to the water content of the raw materials), uniformly mixing, and then putting into a sealed polypropylene box (a hole is formed in the upper cover of the box, and a breathable film is stuck in the hole); placing the culture container filled with culture medium in a sterilizing pot, and sterilizing at 121 deg.C for 30-50 min; after sterilization, the culture vessel was transferred into a buffer chamber, naturally cooled to 24 ℃ or lower, and transferred into an inoculation chamber.
Example 12 solid Medium
The method comprises the following steps of (1) culturing an economic forest branch or stem culture medium: 70% of mulberry twig powder, 20% of elm branch powder, 5% of soybean meal, 5% of corn flour and solid materials: water 1: 1.5 (according to the water content of the raw materials), uniformly mixing, and then putting into a sealed polypropylene box (a hole is formed in the upper cover of the box, and a breathable film is stuck in the hole); placing the culture container filled with culture medium in a sterilizing pot, and sterilizing at 121 deg.C for 30-50 min; after sterilization, the culture vessel was transferred into a buffer chamber, naturally cooled to 24 ℃ or lower, and transferred into an inoculation chamber.
Example 13 Chlorella harvesting and fixation
The liquid culture of the strain of example 1 was inoculated on the wheat medium prepared in example 5, solid culture was carried out as in example 4, and a vertically growing, uniformly growing and highly uniform bundle of sporophores was selected for harvest. Cutting off the sporophore bunch and the solid culture medium together along the direction perpendicular to the culture medium by using a special stainless steel cylinder (one end of which is polished to be sharp and is shown in figure 1) with the diameter of two ends slightly larger than that of a bottle mouth, and then cutting off the culture medium at the lower part by using a paper cutter, wherein the residual thickness is about 0.5cm, and the sporophore bunch cannot be broken in the process. And then placing the coremium fortunei and the solid culture medium in the middle of the wine jar, scattering the coremium fortunei in four directions, and fixing the coremium fortunei at the bottom of the bottle by using a small bamboo stick. Slowly pouring base wine into the jar with culture medium along the bottle wall until the top of the bundle of sporophores is covered by 1-1.5cm, soaking in clean dark room, and aging. After 25 days, the support is removed, and the sporophore bunch and the culture medium can not float upwards again, so that the cordyceps militaris wine with high ornamental value is obtained (see figure 2).
Example 14 Chlorella harvesting and fixation
The seed culture obtained in example 2 was inoculated on the rice medium prepared in example 6, solid culture was carried out as in example 4, and a vertically grown, uniformly grown and highly uniform bundle of sporophores was selected for harvest. The harvesting and fixation method is the same as example 13, except that the solid culture medium is thinned to about 1.0cm, and the support is a glass rod; after 30 days, the support is removed, and the sporophore bundle and the culture medium can not float upwards again, so that the cordyceps militaris wine with high ornamental value is obtained.
Example 15 Chlorella harvesting and fixation
The seed culture obtained in example 3 was inoculated on the brown rice medium prepared in example 7, solid culture was carried out as in example 4, and a vertically growing, uniformly growing and highly uniform bundle of sporophores was selected for harvest. The harvesting and fixation method is the same as example 13, except that the solid medium is thinned to about 1.5cm, and the support is a stainless steel rod; after 35 days, the support is removed, and the sporophore bundle and the culture medium can not float upwards again, so that the cordyceps militaris wine with high ornamental value is obtained.
Example 16 Chlorella harvesting and fixation
The seed culture obtained in example 1 was inoculated on the grain culture medium prepared in example 9, solid culture was carried out as in example 4, and a vertically grown, uniformly grown and highly uniform bundle of sporophores was selected for harvest. The harvesting and fixing method is the same as example 13, after 35 days, the support is removed, the coremium and the culture medium can not float upwards again, and the cordyceps militaris wine with high ornamental value is obtained.
Example 17 Chlorella harvesting and fixation
The strain obtained in example 2 by liquid culture was inoculated on the straw culture medium prepared in example 10, solid culture was carried out as in example 4, and a vertically growing, uniformly growing and highly uniform bundle of sporophores was selected for harvest. The harvesting and fixing method is the same as that of example 14, after 55 days, the support is removed, the coremium and the culture medium can not float upwards again, and the cordyceps militaris wine with high ornamental value is obtained.
Example 18 Chlorella harvesting and fixation
The seed culture obtained in example 3 was inoculated on the husk culture medium prepared in example 11, solid culture was carried out as in example 4, and a vertically grown, uniformly grown and highly uniform bundle of sporophores was selected for harvest. The collecting and fixing method is the same as that in example 15, the support is removed in 60 days, and the coremium and the culture medium can not float upwards again, so that the cordyceps militaris wine with high ornamental value is obtained.
Example 19 Chlorella harvesting and fixation
The strain obtained in example 1 by liquid culture was inoculated on the stalk culture medium prepared in example 12, solid culture was carried out as in example 4, and a vertically grown, uniformly grown and highly uniform bundle of sporophores was selected for harvest. The collecting and fixing method is the same as example 13, the support is removed in 60 days, the coremium and the culture medium can not float upwards again, and the cordyceps militaris wine with high ornamental value is obtained.
Example 20 after ripening
And (3) continuously soaking the cordyceps militaris wine obtained in the embodiment 13-19 for 45-100 days, and taking out of the cellar to obtain the cordyceps militaris wine with high content of nutrient components.
Example 21 comparison of the content of effective ingredients in Cordyceps militaris wine prepared by the method of the present invention and that of the wine prepared by the prior art
Inoculating the strain cultured by the liquid in the example 1 to the wheat culture medium prepared in the example 5, carrying out solid culture according to the example 4, and selecting the sporophores which grow vertically, grow uniformly and have consistent height for harvesting;
sample 1: directly taking off the sporophore bundle, and placing in a wine jar to obtain Cordyceps militaris wine;
sample 2: harvesting and fixing according to the method of the embodiment 13 of the invention to prepare cordyceps militaris wine;
the sample base wine is Gujinggong 50-degree wine.
The contents of adenosine and HEA in samples 1 and 2 were measured periodically (adenosine measuring method is referred to in "high performance liquid chromatography for adenosine measuring method", Baihong Ming's eds. "method for measuring health food efficacy", Chinese medicinal Press, 2011, p214-215 "; HEA measuring method is referred to in" N in Cordyceps militaris fruiting body6Separation and purification of- (2-hydroxyethyl) -adenosine and anti-tumor effect, reported in edible fungi 2013.20 (1): 62-65'). The results are shown in FIGS. 3 to 4.
From FIG. 3, it can be seen that the adenosine content of samples 1 and 2 is highest and level at about 122 days, but the adenosine content of sample 2 is significantly higher than that of sample 1;
from fig. 4 it can be seen that the HEA levels of both samples 1, 2 reached a maximum and leveled off around 105 days, but the HEA level in sample 2 was significantly higher than in sample 1.
As can be seen from fig. 3 and 4, the preparation method of the present invention can leach out the effective components in the sporophores as soon as possible while achieving the ornamental effect, and fig. 3 and 4 can clearly show that the effective component content of sample 2 is significantly higher than that of sample 1 when the effective components are soaked for the same time.
Claims (5)
1. The preparation method of the cordyceps militaris wine is characterized by comprising the following steps of:
step 1, in the solid culture stage of cordyceps militaris, separating part of solid culture medium and the sporophore bundles growing in the solid culture medium from the whole culture medium according to the shape suitable for being placed in a container;
step 2, placing the separated culture medium and the sporophore bundles growing in the culture medium into a container;
step 3, fixing the solid culture medium at the bottom of the container by using a support;
step 4, injecting wine into the container; and
step 5, fixing for 30 days or 35 days, and taking out the support;
wherein the solid culture medium is any one of rice, millet and brown rice culture medium; step 2, placing the solid culture medium in front of a wine container, and thinning the culture medium to the thickness of 1.0cm or 1.5 cm; and 5, taking out the support and continuing to soak for more than 60 days.
2. The method of claim 1, wherein the coreopsis of step 1 is a coreopsis grown to maturity.
3. The method of claim 1, wherein the support in step 3 is a cylindrical structure made of wood, glass, stainless steel or any other material that does not react with the base wine and has a height less than the height from the bottom of the container to the mouth of the container.
4. The method of claim 1, wherein the step 5 comprises removing the support and continuing the soaking for more than 100 days.
5. Cordyceps militaris wine prepared by the method of any one of claims 1 to 4.
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| CN1162015A (en) * | 1997-03-31 | 1997-10-15 | 汪景山 | Production method of wine with shaped Chinese caterpillar fungus soaked in and product thereof |
| CN1954063A (en) * | 2004-07-09 | 2007-04-25 | 李爱玲 | Fresh living cordyceps wine and its production method |
| CN201587938U (en) * | 2009-11-25 | 2010-09-22 | 张笑容 | Wine holding container |
| CN102242044A (en) * | 2010-05-14 | 2011-11-16 | 浙江泛亚生物医药股份有限公司 | Cordyceps cicadae wine and preparation method thereof |
| CN104419602A (en) * | 2013-08-20 | 2015-03-18 | 浙江泛亚生物医药股份有限公司 | Cordyceps sinensis liquor, preparation and application thereof |
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2015
- 2015-10-22 CN CN201510602357.1A patent/CN106609224B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1162015A (en) * | 1997-03-31 | 1997-10-15 | 汪景山 | Production method of wine with shaped Chinese caterpillar fungus soaked in and product thereof |
| CN1954063A (en) * | 2004-07-09 | 2007-04-25 | 李爱玲 | Fresh living cordyceps wine and its production method |
| CN201587938U (en) * | 2009-11-25 | 2010-09-22 | 张笑容 | Wine holding container |
| CN102242044A (en) * | 2010-05-14 | 2011-11-16 | 浙江泛亚生物医药股份有限公司 | Cordyceps cicadae wine and preparation method thereof |
| CN104419602A (en) * | 2013-08-20 | 2015-03-18 | 浙江泛亚生物医药股份有限公司 | Cordyceps sinensis liquor, preparation and application thereof |
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