CN106589162A - Preparation method of chitosan - Google Patents
Preparation method of chitosan Download PDFInfo
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- CN106589162A CN106589162A CN201510686329.2A CN201510686329A CN106589162A CN 106589162 A CN106589162 A CN 106589162A CN 201510686329 A CN201510686329 A CN 201510686329A CN 106589162 A CN106589162 A CN 106589162A
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- China
- Prior art keywords
- preparation
- shitosan
- naoh solution
- chitosan
- neutrality
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to the field of organic polymer compounds, in particular to a preparation method of chitosan. The chitosan can be obtained by conducting drying and crushing, inorganic salt removal, protein removal and acetyl removal on carapace waste. A traditional chitosan preparation method is improved, a simple, convenient and practical chitosan preparation technology is provided, the reaction temperature is raised, the dosage of NaOH is greatly reduced, and the production cost is lowered. In addition, by means of the carapace waste, chitin is purified, chitosan is prepared, the production cost is reduced, the comprehensive utilization value of resources is improved, the environmental pollution is reduced, and environmental protection is promoted.
Description
Technical field
The present invention relates to field of organic polymer compound, and in particular to a kind of preparation method of shitosan.
Background technology
Shitosan is that the chitin being widely present by nature is obtained through deacetylation, is white or faint yellow sheet
Translucent solid, water insoluble and organic solvent, in dissolving in dilute acid soln.Chitin is widely present in shellfish
As in shrimp, the shell of crab, chitin content is 20%～25% wherein in shrimp shell, and chitin content is 15%～20% in crab shell.
China is aquatic products big producing country, and annual discarded shrimp shell, crab shell are a lot, how to purify first from crust discarded object
Shell is plain, prepare shitosan, is the problem that current each scientist competitively studies.
The content of the invention
To solve prior art problem, a kind of preparation method of shitosan is we provided.
The technical solution adopted in the present invention is comprised the following steps：
Step one drying and crushing：Carapace raw material is rinsed well, is crushed with pulverizer after drying naturally；
Step 2 takes off inorganic salts：Dilute HCl is added in crushed material, stirring and leaching under room temperature is filtered with mesh screen, will
Gained filtrate is repeatedly rinsed to neutrality with appropriate distilled water；
Step 3 deproteinization：NaOH solution, heating stirring extraction is added to be filtered with mesh screen in filtrate,
Gained filtrate is repeatedly rinsed to neutrality with appropriate distilled water；
Step 4 deacetylation：NaOH solution is added in filtrate, uses nitrogen soak, reaction to cool down after terminating
To room temperature, screen filtration, neutrality is washed to appropriate distillation, the product Jing high temperature drying after high speed centrifugation is shitosan.
Preferably, in step one, the carapace raw material includes shrimp shell, shrimp tiger shell and crab shell.
Preferably, in step 2, dilute HCl concentration is 1%～10%, and extraction time is 1～2h.
Preferably, in step 3, NaOH solution concentration is 5%～10%, and heating-up temperature is 85～95 DEG C, extraction time
For 2～3h.
Preferably, in step 4, NaOH solution concentration is 60%～80%, and holding temperature is 120～140 DEG C, insulation
Time is 1～2h.
Preferably, in step 2 three or four, filtration mesh screen aperture is 10 mesh.
The beneficial effect that the present invention brings is：
1st, traditional method for preparing chitosan is improved, there is provided more simple and practical Preparing Technology for Chinosan, by improving
Reaction temperature, greatly reduces the usage amount of NaOH, reduces production cost.
2nd, purify chitin, prepare shitosan using crust discarded object, not only save production cost, and improve money
Source comprehensive utilization value, reduces environmental pollution, is conducive to environmental protection.
Specific embodiment
In conjunction with specific embodiment, the present invention will be described in detail：
The example is preferred version, from shrimp shell as preparing raw material, specifically includes following steps：
Step one drying and crushing：Carapace raw material is rinsed well, is crushed with pulverizer after drying naturally；
Step 2 takes off inorganic salts：5%HCl is added in crushed material, stirring and leaching 1.5h under room temperature is entered with 10 mesh mesh screens
Row is filtered, and gained filtrate is repeatedly rinsed to neutrality with appropriate distilled water；
Step 3 deproteinization：7%NaOH solution, 90 DEG C of stirring and leachings 2.5h, with 10 mesh nets are added in filtrate
Sieve is filtered, and gained filtrate is repeatedly rinsed to neutrality with appropriate distilled water；
Step 4 deacetylation：70%NaOH solution is added in filtrate, with 130 DEG C of insulation 1.5h of nitrogen, reaction
Room temperature is cooled to after end, 10 mesh screen filtrations are washed to neutrality, the product Jing high temperature after high speed centrifugation with appropriate distillation
Drying is shitosan.
Above are only a specific embodiment of the present invention, it is clear that the invention is not restricted to above example, can also have
Many deformations.All deformations that one of ordinary skill in the art can directly derive from present disclosure or associate,
It is considered as protection scope of the present invention.
Claims (6)
1. a kind of preparation method of shitosan, it is characterised in that comprise the following steps：
Step one drying and crushing：Carapace raw material is rinsed well, is crushed with pulverizer after drying naturally；
Step 2 takes off inorganic salts：Dilute HCl is added in crushed material, stirring and leaching under room temperature is filtered with mesh screen, will
Gained filtrate is repeatedly rinsed to neutrality with appropriate distilled water；
Step 3 deproteinization：NaOH solution, heating stirring extraction is added to be filtered with mesh screen in filtrate,
Gained filtrate is repeatedly rinsed to neutrality with appropriate distilled water；
Step 4 deacetylation：NaOH solution is added in filtrate, uses nitrogen soak, reaction to cool down after terminating
To room temperature, screen filtration, neutrality is washed to appropriate distillation, the product Jing high temperature drying after high speed centrifugation is shitosan.
2. the preparation method of shitosan according to claim 1, it is characterised in that in the step one, crust is former
Material includes shrimp shell, shrimp tiger shell and crab shell.
3. the preparation method of shitosan according to claim 1, it is characterised in that in the step 2, dilute HCl
Concentration is 1%～10%, and extraction time is 1～2h.
4. the preparation method of shitosan according to claim 1, it is characterised in that in the step 3, NaOH
Solution concentration is 5%～10%, and heating-up temperature is 85～95 DEG C, and extraction time is 2～3h.
5. the preparation method of shitosan according to claim 1, it is characterised in that in the step 4, NaOH
Solution concentration is 60%～80%, and holding temperature is 120～140 DEG C, and temperature retention time is 1～2h.
6. the preparation method of shitosan according to claim 1, it is characterised in that in the step 2 three or four, mistake
Filter mesh screen aperture is 10 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510686329.2A CN106589162A (en) | 2015-10-20 | 2015-10-20 | Preparation method of chitosan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510686329.2A CN106589162A (en) | 2015-10-20 | 2015-10-20 | Preparation method of chitosan |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106589162A true CN106589162A (en) | 2017-04-26 |
Family
ID=58555372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510686329.2A Pending CN106589162A (en) | 2015-10-20 | 2015-10-20 | Preparation method of chitosan |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106589162A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108192122A (en) * | 2017-12-15 | 2018-06-22 | 浙江海洋大学 | A kind of preparation method of MULTILAYER COMPOSITE preservative film |
| CN108586830A (en) * | 2017-12-15 | 2018-09-28 | 浙江海洋大学 | A kind of preparation method of chitosan-gelatin edible freshness-keeping thin coat |
| CN112029014A (en) * | 2020-09-24 | 2020-12-04 | 山东汉方制药有限公司 | Method for extracting chitosan from centipede dregs |
-
2015
- 2015-10-20 CN CN201510686329.2A patent/CN106589162A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108192122A (en) * | 2017-12-15 | 2018-06-22 | 浙江海洋大学 | A kind of preparation method of MULTILAYER COMPOSITE preservative film |
| CN108586830A (en) * | 2017-12-15 | 2018-09-28 | 浙江海洋大学 | A kind of preparation method of chitosan-gelatin edible freshness-keeping thin coat |
| CN108192122B (en) * | 2017-12-15 | 2021-04-20 | 浙江海洋大学 | Preparation method of multilayer composite preservative film |
| CN112029014A (en) * | 2020-09-24 | 2020-12-04 | 山东汉方制药有限公司 | Method for extracting chitosan from centipede dregs |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170426 |