CN106587197B - Nano biological circulating water treatment agent - Google Patents
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Abstract
The invention relates to a nano biological circulating water treatment agent which comprises the following raw materials in parts by mass: 10-20 parts of sepiolite fibers; pure diatom, 100 and 150 portions; 10-20 parts of volcanic charcoal; 60-100 parts of attapulgite; 6-10 parts of zeolite; 10-20 parts of growth promoting enzyme; 0.02-0.07 part of magnesium hydroxide; 0.02-0.07 part of ferric oxide; 30-50 parts of glucose; 50-80 parts of aerobic microbial inoculum; 20-30 parts of anaerobic microbial inoculum; 10-20 parts of a nitrifying bacteria agent; 10-20 parts of denitrifying bacteria agent; 10-20 parts of a phosphorus-accumulating microbial inoculum; 10-20 parts of hydrolytic acidification microbial inoculum; and fully mixing the materials weighed according to the mass proportion, and packaging to obtain the finished product. Can be applied to the treatment of various industrial wastewater which is difficult to degrade, has excellent degradation and removal effects on various pollutants, and reduces the water content of sludge.
Description
Technical Field
The invention relates to a sewage circulating treatment agent.
Background
At present, water consumption of countries in the world is sharply increased, water resource crisis is increasingly severe, and various environmental regulations (water pollution prevention and control law) are formulated successively and are increasingly strict, so that rapid development of environmental protection technology, especially sewage treatment technology, is promoted. The water treatment agent plays a very important role in the water treatment process, and although the water treatment technology comprises a biochemical method, a physical method and a physical-chemical method and continuously has new processes and technologies to be put into the market, the water treatment agent is difficult to completely replace chemical agents. Therefore, water treatment agents and water treatment devices are known as the two wings of the water treatment industry.
With the rapid development of the manufacturing industry in China and the great improvement of the living standard of people, the pollution of natural water caused by excessive water resource development and utilization, industrial production and domestic sewage is more and more serious, and the components of water pollutants are more and more complex, and comprise various organic pollutants, heavy metal elements, high-concentration salts and the like. Although some water treatment agents such as coagulant, coagulant aid and the like are put into use at present, the existing water treatment requirements cannot be met only by the addition of the agents, and particularly, the treatment effect is not ideal for the problems of unstable colloids, ammonia nitrogen and heavy metal ion exceeding standards. Meanwhile, some water treatment agents can generate secondary pollution in the production and use processes, and increase harmful components in the water body. Therefore, it is necessary to develop a sewage treatment agent with high efficiency, wide application range, environmental protection and safety.
Disclosure of Invention
The invention aims to provide a nano biological circulating water treatment agent which can be recycled, has wide applicability, does not cause secondary pollution and reduces the water content of sludge.
The technical scheme adopted by the invention for solving the problems is as follows: a nano biological circulating water treatment agent comprises the following raw materials in parts by mass: 10-20 parts of sepiolite fibers; pure diatom, 100 and 150 portions; 10-20 parts of volcanic charcoal; 60-100 parts of attapulgite; 6-10 parts of zeolite; 10-20 parts of growth promoting enzyme; 0.02-0.07 part of magnesium hydroxide; 0.02-0.07 part of ferric oxide; 30-50 parts of glucose; 50-80 parts of aerobic microbial inoculum; 20-30 parts of anaerobic microbial inoculum; 10-20 parts of a nitrifying bacteria agent; 10-20 parts of denitrifying bacteria agent; 10-20 parts of a phosphorus-accumulating microbial inoculum; 10-20 parts of hydrolytic acidification microbial inoculum; and fully mixing the materials weighed according to the mass proportion, and packaging to obtain the finished product.
The principle and the function of the configuration of each raw material
Sepiolite fibers, volcanic charcoal, attapulgite, zeolite and the like have good adsorption, filtration and other performances, and can effectively remove turbidity, chromaticity, heavy metal ions, organic matters, oils and other pollutants in water; the pure diatom has good adsorption and filtration properties, can promote flocculation and precipitation of pollutants, can effectively improve the property of sludge, enables the sludge formed in the water treatment process to have good dehydration performance, reduces the water content of the sludge, does not need to additionally add a sludge treatment agent in the subsequent treatment, and reduces the subsequent sludge treatment cost; the magnesium hydroxide and the ferric oxide can enhance the flocculation precipitation function; aerobic bacteria, anaerobic bacteria, hydrolytic acidification bacteria and the like degrade pollutants in water through microbial metabolism, nitrobacteria and denitrifying bacteria are mainly used for denitrification, and phosphorus accumulating bacteria are mainly used for phosphorus removal; glucose provides energy for microbial metabolism; the growth promoting enzyme agent is used for promoting the metabolism of microorganisms.
Sepiolite fibers: sepiolite content > 85%, particle size: 250 to 300 mesh, density 2 to 2.5g/cm3，CaO＜1.5%，Fe2O3＜0.03%；
Pure diatom: SiO2 is more than or equal to 88, and the true density is 2.3g/cm3A bulk density of 0.20 to 0.30 g/cm3Fineness: 100-500 meshes;
volcanic charcoal; particle size: 1-2mm, density 0.75g/cm3；
Concave-convex rod: the content of effective substances is more than or equal to 90 percent, and the granularity is as follows: 100 to 200 mesh, and a bulk density of 0.5 + -1 g/cm3The water content is less than or equal to 8 percent;
zeolite: clinoptilolite, particle size: 180-200 mesh, density 1.92g/cm3The ammonia absorption value is more than 100mg equivalent/100 g, and the water content is less than or equal to 1.8 percent.
The preparation method of the aerobic bacterial agent comprises the following steps,
(1) respectively activating and expanding aerobic bacteria in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, wherein the culture temperature is 25-30 ℃ and the culture time is 24-48 hours;
the aerobic bacteria comprise bacillus licheniformis, pseudomonas aeruginosa, nitrosomonas bacterium and sulfur bacterium bailii, and each strain is cultured independently to obtain corresponding bacteria liquid;
(2) respectively measuring each bacterial liquid and inoculating the bacterial liquids into a mixed strain culture medium, wherein the inoculation amount of each bacterial liquid is as follows according to volume percentage: 2% of bacillus licheniformis, 3% of pseudomonas aeruginosa, 2% of nitrosomonas and 4% of sulfobacillus beiensis; the mixed culture temperature is 25-30 ℃, and the culture time is 24-48 hours, so that aerobic bacteria mixed bacterial liquid is obtained;
the mixed strain culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) drying the obtained aerobic bacteria liquid to obtain the aerobic bacteria agent. The drying is centrifugal separation drying or freeze drying.
The preparation method of the anaerobic microbial inoculum comprises the following steps,
(1) respectively activating and expanding anaerobic bacteria strains in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, wherein the culture temperature is 25-30 ℃ and the culture time is 24-48 hours;
the anaerobic bacteria comprise methanogen, methanogen coccus and desulfurization vibrio, and each strain is independently cultured to obtain corresponding bacteria liquid;
(2) respectively measuring each bacterial liquid and inoculating the bacterial liquids into a mixed strain culture medium, wherein the inoculation amount of each bacterial liquid is as follows according to volume percentage: 2% of methanogen bacillus, 3% of methanogen coccus and 2% of desulfurization vibrio; and (3) carrying out mixed culture at the temperature of 25-30 ℃ for 24-48 hours to obtain the anaerobic bacteria mixed bacterial liquid. The mixed strain culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) drying the obtained anaerobic bacteria liquid to obtain the anaerobic bacteria agent.
The preparation method of the nitrifying bacteria agent comprises the following steps,
(1) respectively activating and expanding the nitrobacteria strains in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, wherein the culture temperature is 25-30 ℃ and the culture time is 24-48 hours;
(2) measuring the bacterial liquid and inoculating the bacterial liquid into a strain culture medium, wherein the inoculation amount of the bacterial liquid is 5% by volume; culturing at the temperature of 25-30 ℃ for 24-48 hours to obtain nitrobacteria liquid;
the mass ratio of the strain culture medium is as follows: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of waterSterilizing the nutrient medium at 121 ℃ for 20 minutes;
(3) drying the obtained nitrobacteria liquid to obtain the nitrobacteria agent.
The preparation method of the denitrifying bacteria agent comprises the following steps,
(1) respectively activating and expanding the denitrifying bacteria strains in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, wherein the culture temperature is 25-30 ℃ and the culture time is 24-48 hours;
(2) measuring the bacterial liquid and inoculating the bacterial liquid into a strain culture medium, wherein the inoculation amount of the bacterial liquid is 5% by volume; culturing at the temperature of 25-30 ℃ for 24-48 hours to obtain a denitrifying bacteria liquid;
the mass ratio of the strain culture medium is as follows: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) drying the obtained denitrifying bacteria liquid to obtain the denitrifying bacteria agent.
The preparation method of the polyphosphate microbial inoculum comprises the following steps,
(1) respectively activating and expanding the phosphorus-accumulating bacteria strains in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, wherein the culture temperature is 25-30 ℃ and the culture time is 24-48 hours;
(2) measuring the bacterial liquid and inoculating the bacterial liquid into a strain culture medium, wherein the inoculation amount of the bacterial liquid is 5% by volume; culturing at the temperature of 25-30 ℃ for 24-48 hours to obtain phosphorus-accumulating bacteria liquid;
the mass ratio of the strain culture medium is as follows: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) and drying the obtained phosphorus-accumulating bacterium liquid to obtain the phosphorus-accumulating bacterium agent.
The preparation method of the hydrolytic acidification microbial inoculum comprises the following steps,
(1) respectively activating and expanding the strains of the hydrolytic acidification bacteria in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, wherein the culture temperature is 25-30 ℃ and the culture time is 24-48 hours;
the hydrolytic acidification bacteria strains comprise cellulomonas flavigena, clostridium amyloliquefaciens, bacillus cereus and bacteroides succinogenes, and each strain is cultured independently to obtain corresponding bacteria liquid;
(2) respectively measuring each bacterial liquid and inoculating the bacterial liquids into a mixed strain culture medium, wherein the inoculation amount of each bacterial liquid is as follows according to volume percentage: 2% of cellulomonas flavigena, 3% of clostridium amyloliquefaciens, 2% of bacillus cereus and 4% of bacteroides succinogenes; the mixed culture temperature is 25-30 ℃, and the culture time is 24-48 hours, so as to obtain a mixed bacterial liquid of hydrolytic acidification bacteria;
the mixed strain culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) and drying the obtained hydrolytic acidification bacteria liquid to obtain the hydrolytic acidification bacteria agent.
The growth promoting enzyme agent comprises 1-2 parts of organic carbon source, 1-2 parts of organic nitrogen source, 0.5-1 part of vitamin complex and 1-2 parts of organic acid, and the balance of biological enzyme, wherein the organic acid is one or more than two of citric acid, lactic acid, acetic acid, gluconic acid, malic acid, kojic acid, propionic acid, succinic acid, ascorbic acid and salicylic acid; the biological enzyme is protease, lipase or cellulase. The growth promoting enzyme agent provides nutrient substances for the microorganisms, promotes the growth and the propagation of the microorganisms, can improve the activity of the microorganisms, enables the microorganisms to grow and propagate in a large amount rapidly in a severe environment to form good zoogloea, and improves the degradation capability of the microorganisms on pollutants.
Compared with the prior art, the invention has the advantages that:
(1) various technical principles are integrated, and the application range is wide. Some water treatment agents in the current market have single technical principle and application range, and the treatment effect is not very ideal. The product combines biological, physical and chemical degradation technologies, not only adds professional fungus components suitable for degrading wastewater with various properties, but also has the functions of strengthening chemical reaction, adsorption reaction and flocculation precipitation. Can be applied to the treatment of various industrial wastewater which is difficult to degrade, and has excellent degradation and removal effects on various pollutants.
(2) Green water treatment agent and no secondary pollution. Water treatment agents with similar functions such as polyacrylamide, polyaluminium chloride, various disinfectants and the like in the current market may contain heavy metal elements, residual chlorine, toxic organic solutions and various harmful derivatives in the production process and the use process. The product is safe and reliable, has stable performance, does not produce secondary pollution, belongs to the technical category of green water treatment agents, and accords with the development trend of the current innovative environment-friendly water treatment agents.
(3) Can realize sludge reduction. The generation of a large amount of excess sludge in urban sewage plants is another problem in the field of environmental protection at present, the sludge generated in the general water treatment process is very difficult to dehydrate, the water content of the sludge is very high, the treatment difficulty of the sludge is increased, a medicament needs to be added for treatment, and the problem of secondary pollution exists. If the water treatment agent is added in the treatment process, the flocculation and precipitation speed can be accelerated, the sludge is rapidly coagulated, the dehydration performance is improved, the sludge reduction is realized by 5-10%, harmful substances generated by adding the water treatment agent do not exist in the sludge, and any chemical agent does not need to be added in the sludge treatment process.
(4) The recycling is realized, and the energy consumption is reduced. Some water treatment agent products are large-dose consumables, such as various flocculants, disinfectants and the like, and are continuously consumed in the using process and need to be continuously and quantitatively added. The product can be recycled after being added once, only a small amount of consumption exists in the using process, and only a small amount of medicament needs to be supplemented discontinuously, so that the aims of reducing the water treatment cost, saving energy and reducing consumption while ensuring the water treatment quality requirement are fulfilled.
Detailed Description
The present invention will be described in further detail with reference to examples.
The biological circulating water treating agent mainly comprises sepiolite fibers, pure diatom, volcanic charcoal, attapulgite, zeolite, a growth promoting enzyme agent, magnesium hydroxide, iron oxide, glucose, an aerobic microbial agent, an anaerobic microbial agent, a nitrifying microbial agent, a denitrifying microbial agent, a polyphosphate microbial agent and a hydrolytic acidification microbial agent.
The specific raw material ratios of the examples are shown in table 1.
TABLE 1 Water treatment agent ingredient Table (parts by weight)
The preparation method of the nano biological circulating water treatment agent is characterized in that all prepared raw materials are fully and uniformly mixed and packaged, and the nano biological circulating water treatment agent is directly put into sewage when in use.
The specific culture methods of the aerobic microbial inoculum, the anaerobic microbial inoculum, the nitrifying microbial inoculum, the denitrifying microbial inoculum, the polyphosphate microbial inoculum and the hydrolytic acidification microbial inoculum in the embodiment are as follows
Firstly, a preparation method of an aerobic microbial inoculum comprises the following steps,
(1) respectively activating and expanding aerobic bacteria in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, culturing at the temperature of 28 ℃ for 36 hours;
the aerobic bacteria comprise bacillus licheniformis, pseudomonas aeruginosa, nitrosomonas bacterium and sulfur bacterium bailii, and each strain is cultured independently to obtain corresponding bacteria liquid;
(2) respectively measuring each bacterial liquid and inoculating the bacterial liquids into a mixed strain culture medium, wherein the inoculation amount of each bacterial liquid is as follows according to volume percentage: 2% of bacillus licheniformis, 3% of pseudomonas aeruginosa, 2% of nitrosomonas and 4% of sulfobacillus beiensis; the mixed culture temperature is 25-30 ℃, and the culture time is 24-48 hours, so that aerobic bacteria mixed bacterial liquid is obtained;
the mixed strain culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) drying the obtained aerobic bacteria liquid to obtain an aerobic bacteria agent, and drying by centrifugal separation or freeze drying.
Secondly, the preparation method of the anaerobic microbial inoculum comprises the following steps,
(1) respectively activating and expanding anaerobic bacteria strains in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, culturing at the temperature of 28 ℃ for 36 hours;
the anaerobic bacteria comprise methanogen, methanogen coccus and desulfurization vibrio, and each strain is independently cultured to obtain corresponding bacteria liquid;
(2) respectively measuring each bacterial liquid and inoculating the bacterial liquids into a mixed strain culture medium, wherein the inoculation amount of each bacterial liquid is as follows according to volume percentage: 2% of methanogen bacillus, 3% of methanogen coccus and 2% of desulfurization vibrio; and (3) carrying out mixed culture at the temperature of 25-30 ℃ for 24-48 hours to obtain the anaerobic bacteria mixed bacterial liquid. The mixed strain culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) drying the obtained anaerobic bacteria liquid to obtain the anaerobic bacteria agent.
Thirdly, the preparation method of the nitrifying bacteria agent comprises the following steps,
(1) respectively activating and expanding the nitrobacteria strains in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, culturing at the temperature of 28 ℃ for 36 hours;
(2) measuring the bacterial liquid and inoculating the bacterial liquid into a strain culture medium, wherein the inoculation amount of the bacterial liquid is 5% by volume; culturing at the temperature of 25-30 ℃ for 24-48 hours to obtain nitrobacteria liquid;
the mass ratio of the strain culture medium is as follows: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) drying the obtained nitrobacteria liquid to obtain the nitrobacteria agent.
Fourthly, the preparation method of the denitrifying bacteria agent comprises the following steps,
(1) respectively activating and expanding the denitrifying bacteria strains in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, culturing at the temperature of 28 ℃ for 36 hours;
(2) measuring the bacterial liquid and inoculating the bacterial liquid into a strain culture medium, wherein the inoculation amount of the bacterial liquid is 5% by volume; culturing at the temperature of 25-30 ℃ for 24-48 hours to obtain a denitrifying bacteria liquid;
the mass ratio of the strain culture medium is as follows: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) drying the obtained denitrifying bacteria liquid to obtain the denitrifying bacteria agent.
Fifth, the preparation method of the polyphosphate bacterial agent comprises the following steps,
(1) respectively activating and expanding the phosphorus-accumulating bacteria strains in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutesThe culture temperature is 28 ℃, and the culture time is 36 hours;
(2) measuring the bacterial liquid and inoculating the bacterial liquid into a strain culture medium, wherein the inoculation amount of the bacterial liquid is 5% by volume; culturing at the temperature of 25-30 ℃ for 24-48 hours to obtain phosphorus-accumulating bacteria liquid;
the mass ratio of the strain culture medium is as follows: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) and drying the obtained phosphorus-accumulating bacterium liquid to obtain the phosphorus-accumulating bacterium agent.
Sixthly, a preparation method of the hydrolytic acidification microbial inoculum comprises the following steps,
(1) respectively activating and expanding the strains of the hydrolytic acidification bacteria in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, culturing at the temperature of 28 ℃ for 36 hours;
the hydrolytic acidification bacteria strains comprise cellulomonas flavigena, clostridium amyloliquefaciens, bacillus cereus and bacteroides succinogenes, and each strain is cultured independently to obtain corresponding bacteria liquid;
(2) respectively measuring each bacterial liquid and inoculating the bacterial liquids into a mixed strain culture medium, wherein the inoculation amount of each bacterial liquid is as follows according to volume percentage: 2% of cellulomonas flavigena, 3% of clostridium amyloliquefaciens, 2% of bacillus cereus and 4% of bacteroides succinogenes; the mixed culture temperature is 25-30 ℃, and the culture time is 24-48 hours, so as to obtain a mixed bacterial liquid of hydrolytic acidification bacteria;
the mixed strain culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) and drying the obtained hydrolytic acidification bacteria liquid to obtain the hydrolytic acidification bacteria agent.
The sewage treatment effect of the circulating water treatment agent of the present invention is shown in Table 2
The treatment test is carried out on the waste water of the biochemical pool of the urban comprehensive sewage plant, and the results are shown in the following table:
in addition to the above embodiments, the present invention also includes other embodiments, and any technical solutions formed by equivalent transformation or equivalent replacement should fall within the scope of the claims of the present invention.
Claims (7)
1. A nanometer biological circulating water treatment agent is characterized in that: the raw materials comprise the following components in percentage by mass: 10-20 parts of sepiolite fibers; pure diatom, 100 and 150 portions; 10-20 parts of volcanic charcoal; 60-100 parts of attapulgite; 6-10 parts of zeolite; 10-20 parts of growth promoting enzyme; 0.02-0.07 part of magnesium hydroxide; 0.02-0.07 part of ferric oxide; 30-50 parts of glucose; 50-80 parts of aerobic microbial inoculum; 20-30 parts of anaerobic microbial inoculum; 10-20 parts of a nitrifying bacteria agent; 10-20 parts of denitrifying bacteria agent; 10-20 parts of a phosphorus-accumulating microbial inoculum; 10-20 parts of hydrolytic acidification microbial inoculum;
fully mixing the materials weighed according to the mass proportion and packaging to obtain a finished product;
the preparation method of the aerobic bacterial agent comprises the following steps,
(1) respectively activating and expanding aerobic bacteria in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, wherein the culture temperature is 25-30 ℃ and the culture time is 24-48 hours;
the aerobic bacteria comprise bacillus licheniformis, pseudomonas aeruginosa, nitrosomonas bacterium and sulfur bacterium bailii, and each strain is cultured independently to obtain corresponding bacteria liquid;
(2) respectively measuring each bacterial liquid and inoculating the bacterial liquids into a mixed strain culture medium, wherein the inoculation amount of each bacterial liquid is as follows according to volume percentage: 2% of bacillus licheniformis, 3% of pseudomonas aeruginosa, 2% of nitrosomonas and 4% of sulfobacillus beiensis; the mixed culture temperature is 25-30 ℃, and the culture time is 24-48 hours, so that aerobic bacteria mixed bacterial liquid is obtained;
the mixed strain culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) drying the obtained aerobic bacteria liquid to obtain an aerobic bacteria agent;
the preparation method of the anaerobic microbial inoculum comprises the following steps,
(1) respectively activating and expanding anaerobic bacteria strains in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, wherein the culture temperature is 25-30 ℃ and the culture time is 24-48 hours;
the anaerobic bacteria comprise methanogen, methanogen coccus and desulfurization vibrio, and each strain is independently cultured to obtain corresponding bacteria liquid;
(2) respectively measuring each bacterial liquid and inoculating the bacterial liquids into a mixed strain culture medium, wherein the inoculation amount of each bacterial liquid is as follows according to volume percentage: 2% of methanogen bacillus, 3% of methanogen coccus and 2% of desulfurization vibrio; the mixed culture temperature is 25-30 ℃, and the culture time is 24-48 hours, so that an anaerobic bacteria mixed bacterial liquid is obtained;
the mixed strain culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) drying the obtained anaerobic bacteria liquid to obtain the anaerobic bacteria agent.
2. The nano biological circulating water treatment agent according to claim 1, characterized in that: the preparation method of the nitrifying bacteria agent comprises the following steps,
(1) respectively activating and expanding the nitrobacteria strains in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, wherein the culture temperature is 25-30 ℃ and the culture time is 24-48 hours;
(2) measuring the bacterial liquid and inoculating the bacterial liquid into a strain culture medium, wherein the inoculation amount of the bacterial liquid is 5% by volume; culturing at the temperature of 25-30 ℃ for 24-48 hours to obtain nitrobacteria liquid;
the mass ratio of the strain culture medium is as follows: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) drying the obtained nitrobacteria liquid to obtain the nitrobacteria agent.
3. The nano biological circulating water treatment agent according to claim 1, characterized in that: the preparation method of the denitrifying bacteria agent comprises the following steps,
(1) respectively activating and expanding the denitrifying bacteria strains in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, wherein the culture temperature is 25-30 ℃ and the culture time is 24-48 hours;
(2) measuring the bacterial liquid and inoculating the bacterial liquid into a strain culture medium, wherein the inoculation amount of the bacterial liquid is 5% by volume; culturing at the temperature of 25-30 ℃ for 24-48 hours to obtain a denitrifying bacteria liquid;
the mass ratio of the strain culture medium is as follows: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) drying the obtained denitrifying bacteria liquid to obtain the denitrifying bacteria agent.
4. The nano biological circulating water treatment agent according to claim 1, characterized in that: the preparation method of the polyphosphate microbial inoculum comprises the following steps,
(1) mixing phosphorus-accumulating bacteria strainRespectively carrying out activation and amplification culture in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, wherein the culture temperature is 25-30 ℃ and the culture time is 24-48 hours;
(2) measuring the bacterial liquid and inoculating the bacterial liquid into a strain culture medium, wherein the inoculation amount of the bacterial liquid is 5% by volume; culturing at the temperature of 25-30 ℃ for 24-48 hours to obtain phosphorus-accumulating bacteria liquid;
the mass ratio of the strain culture medium is as follows: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) and drying the obtained phosphorus-accumulating bacterium liquid to obtain the phosphorus-accumulating bacterium agent.
5. The nano biological circulating water treatment agent according to claim 1, characterized in that: the preparation method of the hydrolytic acidification microbial inoculum comprises the following steps,
(1) respectively activating and expanding the strains of the hydrolytic acidification bacteria in a culture medium: the culture medium comprises the following components in percentage by mass: 1% of yeast extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5%、K2HPO40.5 percent of water and the balance of water, sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes, wherein the culture temperature is 25-30 ℃ and the culture time is 24-48 hours;
the hydrolytic acidification bacteria strains comprise cellulomonas flavigena, clostridium amyloliquefaciens, bacillus cereus and bacteroides succinogenes, and each strain is cultured independently to obtain corresponding bacteria liquid;
(2) respectively measuring each bacterial liquid and inoculating the bacterial liquids into a mixed strain culture medium, wherein the inoculation amount of each bacterial liquid is as follows according to volume percentage: 2% of cellulomonas flavigena, 3% of clostridium amyloliquefaciens, 2% of bacillus cereus and 4% of bacteroides succinogenes; the mixed culture temperature is 25-30 ℃, and the culture time is 24-48 hours, so as to obtain a mixed bacterial liquid of hydrolytic acidification bacteria;
the mixed strain culture medium comprises the following components in percentage by mass: yeast1% of extract, 1.5% of peptone, 0.5% of glucose and NH4Cl20.5 percent of water and the balance of water, and sterilizing the culture medium at the high temperature of 121 ℃ for 20 minutes;
(3) and drying the obtained hydrolytic acidification bacteria liquid to obtain the hydrolytic acidification bacteria agent.
6. The nano biological circulating water treatment agent according to claim 1, characterized in that: every 10 parts of growth promoting enzyme agent comprises 1-2 parts of organic carbon source, 1-2 parts of organic nitrogen source, 0.5-1 part of vitamin complex, 1-2 parts of organic acid, and the balance of biological enzyme,
the organic acid is one or more than two of citric acid, lactic acid, acetic acid, gluconic acid, malic acid, kojic acid, propionic acid, succinic acid, ascorbic acid and salicylic acid;
the biological enzyme is protease, lipase or cellulase.
7. The nano biological circulating water treatment agent according to claim 1, characterized in that:
sepiolite fibers: sepiolite content > 85%, particle size: 250 to 300 mesh, density 2 to 2.5g/cm3，CaO＜1.5%，Fe2O3＜0.03%；
Pure diatom: SiO2 is more than or equal to 88, and the true density is 2.3g/cm3A bulk density of 0.20 to 0.30 g/cm3Fineness: 100-500 meshes;
volcanic charcoal: particle size: 1-2mm, density 0.75g/cm3；
Concave-convex rod: the content of effective substances is more than or equal to 90 percent, and the granularity is as follows: 100 to 200 mesh, and a bulk density of 0.5 + -1 g/cm3The water content is less than or equal to 8 percent;
zeolite: clinoptilolite, particle size: 180-200 mesh, density 1.92g/cm3The ammonia absorption value is more than 100mg equivalent/100 g, and the water content is less than or equal to 1.8 percent.
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| CN109502725A (en) * | 2018-11-30 | 2019-03-22 | 江西绿鄱环保科技有限公司 | A kind of waste water dephosphorization agent of high-efficiency low-toxicity and preparation method thereof |
| CN111718008A (en) * | 2020-06-11 | 2020-09-29 | 中石大蓝天(青岛)石油技术有限公司 | Water environment pollution control method |
| CN112573669A (en) * | 2020-11-20 | 2021-03-30 | 广州绵源环境技术有限公司 | Complex microbial inoculant |
| CN115180725A (en) * | 2022-08-02 | 2022-10-14 | 中绿时代(北京)生物科技有限公司 | Biological agent for sewage treatment BEBR (biological aerated biofilm reactor) system and application |
| CN114956338B (en) * | 2022-08-02 | 2022-10-21 | 中绿时代(北京)生物科技有限公司 | Biological nitrogen and phosphorus removal composition for sewage treatment BEBR (biological nitrogen and phosphorus removal) system and application |
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