CN106579328A - Compound polysaccharide antioxidant - Google Patents
Compound polysaccharide antioxidant Download PDFInfo
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- CN106579328A CN106579328A CN201611153014.2A CN201611153014A CN106579328A CN 106579328 A CN106579328 A CN 106579328A CN 201611153014 A CN201611153014 A CN 201611153014A CN 106579328 A CN106579328 A CN 106579328A
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- polysaccharide
- antioxidant
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- composite antioxidant
- herba
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- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 70
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 70
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 41
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 38
- -1 Compound polysaccharide Chemical class 0.000 title claims abstract description 15
- 150000004676 glycans Chemical class 0.000 claims abstract description 58
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 8
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 8
- 244000269722 Thea sinensis Species 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 14
- 239000002131 composite material Substances 0.000 claims description 13
- 238000001035 drying Methods 0.000 claims description 13
- 241001247821 Ziziphus Species 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 241001660920 Agrocybe chaxingu Species 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 6
- UAJTZZNRJCKXJN-UHFFFAOYSA-M sodium;2-dodecoxy-2-oxoethanesulfonate Chemical compound [Na+].CCCCCCCCCCCCOC(=O)CS([O-])(=O)=O UAJTZZNRJCKXJN-UHFFFAOYSA-M 0.000 claims description 6
- 206010040844 Skin exfoliation Diseases 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 5
- 238000007560 sedimentation technique Methods 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 3
- 241000251468 Actinopterygii Species 0.000 claims 1
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 235000009024 Ceanothus sanguineus Nutrition 0.000 claims 1
- 240000003553 Leptospermum scoparium Species 0.000 claims 1
- 235000015459 Lycium barbarum Nutrition 0.000 claims 1
- 239000012141 concentrate Substances 0.000 claims 1
- 235000020995 raw meat Nutrition 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 7
- 235000013373 food additive Nutrition 0.000 abstract description 2
- 239000002778 food additive Substances 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 244000045069 Agrocybe aegerita Species 0.000 abstract 1
- 230000002508 compound effect Effects 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 229940092258 rosemary extract Drugs 0.000 abstract 1
- 235000020748 rosemary extract Nutrition 0.000 abstract 1
- 239000001233 rosmarinus officinalis l. extract Substances 0.000 abstract 1
- 235000006708 antioxidants Nutrition 0.000 description 23
- 238000007254 oxidation reaction Methods 0.000 description 10
- 239000003795 chemical substances by application Substances 0.000 description 9
- 230000003647 oxidation Effects 0.000 description 9
- BGNXCDMCOKJUMV-UHFFFAOYSA-N Tert-Butylhydroquinone Chemical compound CC(C)(C)C1=CC(O)=CC=C1O BGNXCDMCOKJUMV-UHFFFAOYSA-N 0.000 description 8
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 8
- 229960004756 ethanol Drugs 0.000 description 8
- 230000003449 preventive effect Effects 0.000 description 8
- 239000000523 sample Substances 0.000 description 6
- 235000013616 tea Nutrition 0.000 description 6
- 230000031700 light absorption Effects 0.000 description 4
- 239000004250 tert-Butylhydroquinone Substances 0.000 description 4
- 235000019281 tert-butylhydroquinone Nutrition 0.000 description 4
- 239000012488 sample solution Substances 0.000 description 3
- 229960000935 dehydrated alcohol Drugs 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000007760 free radical scavenging Effects 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 201000010235 heart cancer Diseases 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 208000024348 heart neoplasm Diseases 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 235000020124 milk-based beverage Nutrition 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention discloses a compound polysaccharide antioxidant and belongs to the technical field of food additives. The compound polysaccharide antioxidant comprises 40-60 parts of polysaccharides, 20-30 parts of a rosemary extract and 10-20 parts of tea polyphenols. The polysaccharides are water-soluble polysaccharides and one or more of herba houttuyniae polysaccharides or agrocybe cylindracea polysaccharides or red date polysaccharides. The prepared antioxidant has the same antioxidant effect as synthetic antioxidants and at the same time increases the nutritional value of food. The compound effects of the provided compound antioxidant are not simply superimposed and the compound antioxidant has the synergistic effects.
Description
Technical field
The present invention relates to a kind of antioxidant and preparation method thereof, belongs to technical field of food additives.
Background technology
The antioxidant all more or less contained in milk beverage on the market, tea beverage and fruit drink, conventional antioxygen
Agent has BHA, BHT, PG, TBHQ, but these antioxidants are all synthetized oxidation preventive agents, as people's property safe to food is required
Raising and the security incident doubt of chemosynthesis antioxidant is increased, people wait in expectation and develop safety and efficient natural
Antioxidant, the current Natural antioxidant has vitamin A, C, E, P, polyphenol, flavone etc., and these antioxidants can help the mankind
The various diseases of prevention heart disease and cancer etc., and mental, slow down aging can be promoted, but the Natural antioxidant is added in food
Imperfection is gone back in research, and the Natural antioxidant can't reach the antioxidant effect that synthetized oxidation preventive agent has, and limits natural anti-
The use of oxidant, prevents it from substituting synthetized oxidation preventive agent completely.
The content of the invention
It is an object of the invention to provide a kind of antioxidant and preparation method thereof, solves the Natural antioxidant in prior art
The technical problem of synthetized oxidation preventive agent can not completely be substituted.
A kind of polysaccharide composite antioxidant, the polysaccharide comprising 40-60 parts, the Herba Rosmarini Officinalis extract of 20-30 parts and 10-20 parts
Tea polyphenols.
The polysaccharide is water soluble polysaccharide.
The polysaccharide is one or more of Herba Houttuyniae polysaccharide either Methods of Polysaccharide From Agrocybe Chaxingu or jujube polysaccharide.
The Herba Houttuyniae polysaccharide, Methods of Polysaccharide From Agrocybe Chaxingu, the extracting mode of jujube polysaccharide are decoction and alcohol sedimentation technique；
The de- core of Fructus Jujubae Jing peelings；Herba Houttuyniae is cleaned up；Agrocyb eaegerita removes mushroom foot, cleans up；The Jing peelings take off core
Fructus Jujubae, the Herba Houttuyniae for cleaning up and remove mushroom foot and the agrocyb eaegerita for cleaning up, dried respectively, crush, obtain jujube polysaccharide,
Herba Houttuyniae polysaccharide and Methods of Polysaccharide From Agrocybe Chaxingu；
The drying is to dry 12-24h with 40-50 DEG C of baking oven, and 80 mesh sieves are crossed after the crushing.
The extracting method of the Herba Rosmarini Officinalis extract is size-reduced to take Herba Rosmarini Officinalis cured leaf, sieves, and is extracted with ethanol solution, carries
Take liquid it is concentrated, dry Herba Rosmarini Officinalis extract.
Described to sieve to cross 80 mesh sieves, the ethanol solution concentration is 60-70%, and solid-liquid ratio is 1:(5-10) it is, described dense
50-60 DEG C of concentrating under reduced pressure is condensed to, the drying adopts lyophilization.
Beneficial effects of the present invention：The present invention prepares antioxidant, and antioxidant effect is suitable with synthetized oxidation preventive agent, and energy
Long-time ensures to act on the oxidation protection of food, while increased nutritive value of food, the TBHQ of 1mg/mL to DPPH from
By clearance rate be 78.12%, be 75.36% to the clearance rate of hydroxyl radical free radical, antioxidant prepared by the present invention is same dense
When spending, 86.74% is up to the free clearance rate of DPPH, 89.87% is up to the clearance rate of hydroxyl radical free radical, with synthesis
The antioxidant effect of antioxidant quite, can completely substitute synthetized oxidation preventive agent and be added in food, simultaneously as adding many
Sugar, improves nutritive value of food.
Specific embodiment
The assay method of DPPH free radical scavenging activities
The measure of DPPH free radical scavenging activities adds sample solution and 0.1mmol/L DPPH solution each in test tube
2mL, vortex oscillation is mixed, and 30min is reacted in darkroom, and the absorbance surveyed at 517nm wavelength simultaneously calculates clearance rate.
It is blank：Dehydrated alcohol replaces DPPH, water to replace sample solution；A1For the light absorption value of sample；A0It is that sample is replaced with water
The light absorption value of the control experiment of solution；A2It is the light absorption value of the sample interference experiment for replacing DPPH solution with dehydrated alcohol.
The measure of hydroxy radical (OH) clearance rate
H2O2With Fe2+Generation Fenton's reaction generates OH, so as to orthophenanthroline-Fe2+It is oxidized to orthophenanthroline-Fe3 +[98], when there is antioxidant in system, this oxidation reaction can be made suppressed, sequentially add orthophenanthroline in 10mL test tubes
(5mmol/L) 1.0mL, PBS (0.2mol/L pH7.4) 2.0mL, sample solution 1.0mL, H2O2(0.1%v/v)
1.0mL, 37 DEG C of water-bath 1h.
A0：It is blank that distilled water replaces sample；A1：The light absorption value of sample；A2：Distilled water replaces in addition to sample
Material；Ultraviolet-visible spectrophotometer wavelength is 536nm.
Embodiment 1：
A kind of polysaccharide composite antioxidant, the polysaccharide comprising 50 parts, 30 parts of Herba Rosmarini Officinalis extract and 20 parts of tea polyphenols.
The polysaccharide is water soluble polysaccharide.
The polysaccharide is jujube polysaccharide.
The extracting mode of the jujube polysaccharide is decoction and alcohol sedimentation technique；
The de- core of Fructus Jujubae Jing peelings, drying is crushed, and obtains jujube polysaccharide；
The drying is to dry 16h with 50 DEG C of baking ovens, and 80 mesh sieves are crossed after the crushing.
The extracting method of the Herba Rosmarini Officinalis extract is size-reduced to take Herba Rosmarini Officinalis cured leaf, sieves, and is extracted with ethanol solution, carries
Take liquid it is concentrated, dry Herba Rosmarini Officinalis extract.
Described to sieve to cross 80 mesh sieves, the ethanol solution concentration is 65%, and solid-liquid ratio is 1:8, the concentration is 50 DEG C
Concentrating under reduced pressure, the drying adopts lyophilization.
Embodiment 2：
A kind of polysaccharide composite antioxidant, the polysaccharide comprising 60 parts, 30 parts of Herba Rosmarini Officinalis extract and 10 parts of tea polyphenols.
The polysaccharide is water soluble polysaccharide.
The polysaccharide is Herba Houttuyniae polysaccharide.
The extracting mode of the Herba Houttuyniae polysaccharide is decoction and alcohol sedimentation technique；
Herba Houttuyniae is cleaned up, drying, is crushed, and obtains Herba Houttuyniae polysaccharide；
The drying is to dry 20h with 45 DEG C of baking ovens, and 80 mesh sieves are crossed after the crushing.
The extracting method of the Herba Rosmarini Officinalis extract is size-reduced to take Herba Rosmarini Officinalis cured leaf, sieves, and is extracted with ethanol solution, carries
Take liquid it is concentrated, dry Herba Rosmarini Officinalis extract.
Described to sieve to cross 80 mesh sieves, the ethanol solution concentration is 60%, and solid-liquid ratio is 1:5, the concentration is 50 DEG C
Concentrating under reduced pressure, the drying adopts lyophilization.
Embodiment 3：
A kind of polysaccharide composite antioxidant, the polysaccharide comprising 50 parts, 30 parts of Herba Rosmarini Officinalis extract and 20 parts of tea polyphenols.
The polysaccharide is water soluble polysaccharide.
The polysaccharide is Methods of Polysaccharide From Agrocybe Chaxingu.
The extracting mode of Methods of Polysaccharide From Agrocybe Chaxingu is decoction and alcohol sedimentation technique；
Agrocyb eaegerita removes mushroom foot, cleans up, drying, crushes, Methods of Polysaccharide From Agrocybe Chaxingu；
The drying is to dry 12h with 50 DEG C of baking ovens, and 80 mesh sieves are crossed after the crushing.
The extracting method of the Herba Rosmarini Officinalis extract is size-reduced to take Herba Rosmarini Officinalis cured leaf, sieves, and is extracted with ethanol solution, carries
Take liquid it is concentrated, dry Herba Rosmarini Officinalis extract.
Described to sieve to cross 80 mesh sieves, the ethanol solution concentration is 70%, and solid-liquid ratio is 1:10, the concentration is 60 DEG C
Concentrating under reduced pressure, the drying adopts lyophilization.
Embodiment 4：Antioxidant activity is contrasted
Take TBHQ, jujube polysaccharide, Herba Houttuyniae polysaccharide, Methods of Polysaccharide From Agrocybe Chaxingu, Herba Rosmarini Officinalis extract, tea polyphenols and embodiment 1,
Antioxidant prepared by embodiment 2 and embodiment 3, is all configured to the solution of 1mg/mL, determines them to DPPH, hydroxyl radical free radical
Clearance rate evaluating its antioxidant activity.As a result it is as shown in the table：
As seen from the table, the antioxidant antioxidant effect prepared by the present invention is imitated with synthetized oxidation preventive agent TBHQ antioxidation
Fruit quite, can completely substitute synthetized oxidation preventive agent and be added in beverage, and the effect of above-mentioned antioxidant compounding is not simple
Superposition, but there occurs synergistic function.
Above preferred embodiment is merely to illustrate present disclosure, and in addition, the present invention also has other embodiment,
In every case those skilled in the art are because of technology enlightenment involved in the present invention, and adopt what equivalent or equivalent deformation mode were formed
Technical scheme is all fallen within protection scope of the present invention.
Claims (8)
1. a kind of polysaccharide composite antioxidant, it is characterised in that the polysaccharide comprising 40-60 parts, the Herba Rosmarini Officinalis extract of 20-30 parts
With the tea polyphenols of 10-20 parts.
2. a kind of polysaccharide composite antioxidant according to claim 1, it is characterised in that the polysaccharide is that water solublity is more
Sugar.
3. a kind of polysaccharide composite antioxidant according to claim 2, it is characterised in that the polysaccharide is that Herba Houttuyniae is more
One or more in sugar, Methods of Polysaccharide From Agrocybe Chaxingu or jujube polysaccharide.
4. a kind of polysaccharide composite antioxidant according to Claims 2 or 3, it is characterised in that the Herba Houttuyniae polysaccharide, tea
Tree mushroom polysaccharide, the extracting mode of jujube polysaccharide are decoction and alcohol sedimentation technique, are concretely comprised the following steps：
The de- core of Fructus Jujubae Jing peelings；Herba Houttuyniae is cleaned up；Agrocyb eaegerita removes mushroom foot, cleans up；The Fructus Jujubae of the de- core of Jing peeling,
The Herba Houttuyniae that cleans up and mushroom foot and the agrocyb eaegerita for cleaning up are removed, dried respectively, crushed, obtain jujube polysaccharide, fish raw meat
Grass polysaccharide and Methods of Polysaccharide From Agrocybe Chaxingu；
5. a kind of polysaccharide composite antioxidant according to claim 4, it is characterised in that the drying is with 40-50 DEG C
Baking oven dries 12-24h, and 80 mesh sieves are crossed after the crushing.
6. a kind of polysaccharide composite antioxidant according to claim 1, it is characterised in that the Herba Rosmarini Officinalis extract is carried
It is size-reduced to take Herba Rosmarini Officinalis cured leaf to take method, sieves, and is extracted with ethanol solution, and extracting solution is concentrated, dry that Herba Rosmarini Officinalis is extracted
Thing.
7. a kind of polysaccharide composite antioxidant according to claim 1, it is characterised in that described to sieve to cross 80 mesh sieves,
The ethanol solution concentration is 60-70%, and solid-liquid ratio is 1:(5-10) it is, described to concentrate as 50-60 DEG C of concentrating under reduced pressure, the drying
Using lyophilization.
8. according to claim 1-3, the application of the arbitrary described polysaccharide composite antioxidants of 5-7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611153014.2A CN106579328A (en) | 2016-12-14 | 2016-12-14 | Compound polysaccharide antioxidant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611153014.2A CN106579328A (en) | 2016-12-14 | 2016-12-14 | Compound polysaccharide antioxidant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106579328A true CN106579328A (en) | 2017-04-26 |
Family
ID=58802425
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611153014.2A Pending CN106579328A (en) | 2016-12-14 | 2016-12-14 | Compound polysaccharide antioxidant |
Country Status (1)
| Country | Link |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1466971A (en) * | 2002-07-08 | 2004-01-14 | 贵州昱华实业有限公司产品技术研究所 | Production process for extracting liposoluble and water soluble antioxidant from rosemary |
| JP2006282740A (en) * | 2005-03-31 | 2006-10-19 | Mitsubishi Chemicals Corp | Deterioration-preventive agent |
| CN101803787A (en) * | 2010-04-15 | 2010-08-18 | 周杰超 | High-efficiency compound food antioxidant |
| CN103478235A (en) * | 2013-09-17 | 2014-01-01 | 南京通泽农业科技有限公司 | Strawberry anti-staling agent |
| CN103767042A (en) * | 2014-01-03 | 2014-05-07 | 广东广益科技实业有限公司 | Natural antioxidant and preparation method natural antioxidant |
| CN104027479A (en) * | 2014-05-26 | 2014-09-10 | 广东粤微食用菌技术有限公司 | Glossy ganoderma-tea polyphenol composite antioxidant |
| CN104757694A (en) * | 2015-05-06 | 2015-07-08 | 赵志坚 | Natural antioxidant |
-
2016
- 2016-12-14 CN CN201611153014.2A patent/CN106579328A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1466971A (en) * | 2002-07-08 | 2004-01-14 | 贵州昱华实业有限公司产品技术研究所 | Production process for extracting liposoluble and water soluble antioxidant from rosemary |
| JP2006282740A (en) * | 2005-03-31 | 2006-10-19 | Mitsubishi Chemicals Corp | Deterioration-preventive agent |
| CN101803787A (en) * | 2010-04-15 | 2010-08-18 | 周杰超 | High-efficiency compound food antioxidant |
| CN103478235A (en) * | 2013-09-17 | 2014-01-01 | 南京通泽农业科技有限公司 | Strawberry anti-staling agent |
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