CN106578961A - Method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using fermentation method - Google Patents

Method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using fermentation method Download PDF

Info

Publication number
CN106578961A
CN106578961A CN201611146609.5A CN201611146609A CN106578961A CN 106578961 A CN106578961 A CN 106578961A CN 201611146609 A CN201611146609 A CN 201611146609A CN 106578961 A CN106578961 A CN 106578961A
Authority
CN
China
Prior art keywords
semen
fermentation
peptide
oral liquid
semen phaseoli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201611146609.5A
Other languages
Chinese (zh)
Inventor
张莉莉
姚鑫淼
卢淑雯
季妮娜
管立军
李哲滨
赵蕊
张英蕾
沈卉芳
周野
崔怡娟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
FOOD PROCESSING INST OF HEILONGJIANG PROV AGRICULTURAL SCIENCES ACADEMY
Original Assignee
FOOD PROCESSING INST OF HEILONGJIANG PROV AGRICULTURAL SCIENCES ACADEMY
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by FOOD PROCESSING INST OF HEILONGJIANG PROV AGRICULTURAL SCIENCES ACADEMY filed Critical FOOD PROCESSING INST OF HEILONGJIANG PROV AGRICULTURAL SCIENCES ACADEMY
Priority to CN201611146609.5A priority Critical patent/CN106578961A/en
Publication of CN106578961A publication Critical patent/CN106578961A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Medicines Containing Plant Substances (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to a method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using a fermentation method. Small red beans are fermented by aspergillus oryzae, functional peptide and DPP-4 are obtained by a microbial fermentation means, and the red bean peptide oral liquid is prepared. The method has the advantages that in the preparation process of the red bean peptide, the flavor of substances generated by microbial fermentation is better than the flavor of substances generated by enzymatic hydrolysis, the safety is high, the peptide yield is high, and the shortcoming that functional substances are bitter in a process of enzymatic hydrolysis is further overcome; and compared with enzymatic hydrolysis, microbial fermentation is shorter in period, easier to control and lower in cost.

Description

A kind of fermentation method makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect
Technical field
The present invention relates to a kind of Semen Phaseoli peptide oral liquid manufacture method, and in particular to a kind of fermentation method makes has reducing pressure and sugar The Semen Phaseoli peptide oral liquid method of effect.
Background technology
At present preparations of country's Semen Phaseoli peptide be by way of enzymolysis obtaining, but using enzymatic isolation method with as follows Shortcoming:
1. during adopting the Semen Phaseoli peptide of enzyme hydrolysiss legal system, bitter substance saponin can be supervened.
2. enzymolysis process is wayward.
3. the Semen Phaseoli peptide yield by enzyme digestion reaction generation is relatively low.
The content of the invention
The purpose of the present invention is for deficiency of the prior art, there is provided a kind of fermentation method makes has reducing pressure and sugar effect Semen Phaseoli peptide oral liquid method, the cycle is shorter, is more easy to manipulation, and cost is lower.
For achieving the above object, the invention discloses following technical scheme:
A kind of fermentation method makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, is fermented by aspergillus oryzae red little Bean, using the means of fermentable functional peptide and DPP-4 are obtained, and Semen Phaseoli peptide oral liquid is obtained.
Further, Semen Ormosiae Hosiei is made into Semen Ormosiae Hosiei mud before fermenting.
Further, comprising the following steps that for Semen Ormosiae Hosiei mud is made:
Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, 100-150 DEG C of autoclave, 20-40min sterilizings is placed on.
Further, aspergillus oryzae is carried out into process activation before fermenting.
Further, comprising the following steps that for aspergillus oryzae is activated:Aspergillus oryzae is activated under conditions of 30-35 DEG C 40- 50h。
Further, the aspergillus oryzae after activation is diluted in the case of without miscellaneous bacteria and is sprayed on Semen Ormosiae Hosiei mud, 20-35 DEG C Fermentation 72h, takes 50-80 parts fermented product and mixes with 120-180 part distilled water and stir evenly, and 80-100 DEG C is heated 20-45min, cooled Filtering must contain the solution of Semen Phaseoli peptide, wherein, fermented product and distilled water are measured using parts by weight.
Further, comprise the following steps that:
Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, pH value is adjusted to 6.0,121 DEG C of autoclave, 30min is placed on Sterilizing, water-bath be cooled to 30 DEG C it is standby, using PDA slant culture, aspergillus oryzae strain is taken into 3 inoculating loops and is inoculated in culture medium, 31 DEG C, it is inoculated in triangular flask after culture 48h and is enlarged culture, then uses the aspergillus oryzae for having activated in the case of without miscellaneous bacteria Physiological saline solution washes lower spore, and concentration is adjusted to into 108Individual/mL, sprays on Semen Ormosiae Hosiei mud, and spray flux is every 1000g Semen Ormosiae Hosiei mud sprays 3mL bacterium solutions, 30 DEG C of Jing fermentation 72h, takes 75 parts of fermented products and mixes with 150 parts of distilled water and stirs evenly, and 90 DEG C are heated 30min, cold filtration must contain the solution of Semen Phaseoli peptide, wherein, fermented product and distilled water are measured using parts by weight.
A kind of fermentation method disclosed by the invention makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, with following Beneficial effect:
1. in the preparation process of Semen Phaseoli peptide, fermentable is more preferable than the material local flavor that enzyme digestion reaction is produced, safety Higher, peptide yield is higher, while also solving the shortcoming of functional mass during enzyme digestion reaction with bitterness.
2. fermentable is compared with enzyme digestion reaction, and the cycle is shorter, is more easy to manipulation, and cost is lower.
Description of the drawings
Fig. 1 is the process chart of the present invention.
Specific embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described enforcement Example is only a part of embodiment of the invention, rather than the embodiment of whole.Based on the embodiment in the present invention, this area is common The every other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model of present invention protection Enclose.
The core of the present invention is to provide a kind of fermentation method and makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its Safe, peptide yield is high, the functional oral liquid feature containing Semen Ormosiae Hosiei peptide is made with it and local flavor is more preferable.
Refer to Fig. 1.
Embodiment 1
A kind of fermentation method makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, is fermented by aspergillus oryzae red little Bean, using the means of fermentable functional peptide and DPP-4 are obtained, and Semen Phaseoli peptide oral liquid is obtained.
As specific embodiment, Semen Ormosiae Hosiei is made into Semen Ormosiae Hosiei mud before fermentation, make comprising the following steps that for Semen Ormosiae Hosiei mud: Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, 100 DEG C of autoclave, 20min sterilizings is placed on.
As specific embodiment, aspergillus oryzae is carried out into process activation before fermentation, activate comprising the following steps that for aspergillus oryzae:Will Aspergillus oryzae activates 40h under conditions of 30 DEG C.
As specific embodiment, the aspergillus oryzae after activation is diluted in the case of without miscellaneous bacteria and is sprayed on Semen Ormosiae Hosiei mud, 20 DEG C of fermentation 72h, take 50 parts of fermented products and mix with 120 parts of distilled water and stir evenly, and 80 DEG C are heated 20min, and cold filtration must contain Semen Phaseoli The solution of peptide, wherein, fermented product and distilled water are measured using parts by weight.
As specific embodiment, comprise the following steps that:Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, PH is adjusted Be worth to 6.0, be placed on 100 DEG C of autoclave, 20min sterilizings, water-bath be cooled to 30 DEG C it is standby, using PDA slant culture, by aspergillus oryzae Strain takes 3 inoculating loops and is inoculated in culture medium, 30 DEG C, is inoculated in triangular flask after culture 40h and is enlarged culture, then will activation Good aspergillus oryzae washes lower spore in the case of without miscellaneous bacteria with physiological saline solution, and its concentration is adjusted to into 108Individual/mL, spray Drench on Semen Ormosiae Hosiei mud, spray flux is every 1000g Semen Ormosiae Hosiei mud spray 3mL bacterium solutions, 20 DEG C of fermentation 72h of Jing take 50 parts of fermented products Mix with 120 parts of distilled water and stir evenly, 80 DEG C of heating 20min, cold filtration must contain the solution of Semen Phaseoli peptide, wherein, fermented product and steaming Distilled water is measured using parts by weight.
Embodiment 2
A kind of fermentation method makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, is fermented by aspergillus oryzae red little Bean, using the means of fermentable functional peptide and DPP-4 are obtained, and Semen Phaseoli peptide oral liquid is obtained.
As specific embodiment, Semen Ormosiae Hosiei is made into Semen Ormosiae Hosiei mud before fermentation, make comprising the following steps that for Semen Ormosiae Hosiei mud: Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, 150 DEG C of autoclave, 40min sterilizings is placed on.
As specific embodiment, aspergillus oryzae is carried out into process activation before fermentation, activate comprising the following steps that for aspergillus oryzae:Will Aspergillus oryzae activates 50h under conditions of 35 DEG C.
As specific embodiment, the aspergillus oryzae after activation is diluted in the case of without miscellaneous bacteria and is sprayed on Semen Ormosiae Hosiei mud, 35 DEG C of fermentation 72h, take 80 parts of fermented products and mix with 180 parts of distilled water and stir evenly, and 100 DEG C are heated 45min, and cold filtration must contain Semen Phaseoli The solution of peptide, wherein, fermented product and distilled water are measured using parts by weight.
As specific embodiment, comprise the following steps that:Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, PH is adjusted Be worth to 6.0, be placed on 150 DEG C of autoclave, 40min sterilizings, water-bath be cooled to 30 DEG C it is standby, using PDA slant culture, by aspergillus oryzae Strain takes 3 inoculating loops and is inoculated in culture medium, 35 DEG C, is inoculated in triangular flask after culture 50h and is enlarged culture, then will activation Good aspergillus oryzae washes lower spore in the case of without miscellaneous bacteria with physiological saline solution, and its concentration is adjusted to into 108Individual/mL, spray Drench on Semen Ormosiae Hosiei mud, spray flux is every 1000g Semen Ormosiae Hosiei mud spray 3mL bacterium solutions, 30 DEG C of fermentation 72h of Jing take 80 parts of fermented products Mix with 180 parts of distilled water and stir evenly, 100 DEG C of heating 45min, cold filtration must contain the solution of Semen Phaseoli peptide, wherein, fermented product and steaming Distilled water is measured using parts by weight.
Embodiment 3
A kind of fermentation method makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, is fermented by aspergillus oryzae red little Bean, using the means of fermentable functional peptide and DPP-4 are obtained, and Semen Phaseoli peptide oral liquid is obtained.
As specific embodiment, Semen Ormosiae Hosiei is made into Semen Ormosiae Hosiei mud before fermentation, make comprising the following steps that for Semen Ormosiae Hosiei mud: Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, 121 DEG C of autoclave, 30min sterilizings is placed on.
As specific embodiment, aspergillus oryzae is carried out into process activation before fermentation, activate comprising the following steps that for aspergillus oryzae:Will Aspergillus oryzae activates 48h under conditions of 31 DEG C.
As specific embodiment, the aspergillus oryzae after activation is diluted in the case of without miscellaneous bacteria and is sprayed on Semen Ormosiae Hosiei mud, 30 DEG C of fermentation 72h, take 75 parts of fermented products and mix with 150 parts of distilled water and stir evenly, and 90 DEG C are heated 30min, and cold filtration must contain Semen Phaseoli The solution of peptide, wherein, fermented product and distilled water are measured using parts by weight.
As specific embodiment, comprise the following steps that:Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, PH is adjusted Be worth to 6.0, be placed on 121 DEG C of autoclave, 30min sterilizings, water-bath be cooled to 30 DEG C it is standby, using PDA slant culture, by aspergillus oryzae Strain takes 3 inoculating loops and is inoculated in culture medium, 31 DEG C, is inoculated in triangular flask after culture 48h and is enlarged culture, then will activation Good aspergillus oryzae washes lower spore in the case of without miscellaneous bacteria with physiological saline solution, and its concentration is adjusted to into 108Individual/mL, spray Drench on Semen Ormosiae Hosiei mud, spray flux is every 1000g Semen Ormosiae Hosiei mud spray 3mL bacterium solutions, 30 DEG C of fermentation 72h of Jing take 75 parts of fermented products Mix with 150 parts of distilled water and stir evenly, 90 DEG C of heating 30min, cold filtration must contain the solution of Semen Phaseoli peptide, wherein, fermented product and steaming Distilled water is measured using parts by weight.
The content introduced in background technology is compared, more preferably, safety is higher, peptide for Semen Phaseoli peptide material local flavor prepared by the present invention Yield is higher, while the shortcoming of functional mass during enzyme digestion reaction with bitterness is also solved, and compared with enzyme digestion reaction, this The invention cycle is shorter, is more easy to manipulation, and cost is lower.
The above is only the preferred embodiment of the present invention, rather than a limitation;Although it should be pointed out that with reference to above-mentioned each Embodiment has been described in detail to the present invention, it will be understood by those within the art that, it still can be to above-mentioned each Technical scheme described in embodiment is modified, or carries out equivalent to which part or all technical characteristic;And this A little modifications and replacement, do not make the scope of the essence disengaging various embodiments of the present invention technical scheme of corresponding technical scheme.

Claims (7)

1. a kind of fermentation method makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, it is characterised in that by aspergillus oryzae Fermentation Semen Ormosiae Hosiei, using the means of fermentable functional peptide and DPP-4 are obtained, and Semen Phaseoli peptide oral liquid is obtained.
2. a kind of fermentation method according to claim 1 makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its It is characterised by, Semen Ormosiae Hosiei is made into Semen Ormosiae Hosiei mud before fermentation.
3. a kind of fermentation method according to claim 2 makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its It is characterised by, makes comprising the following steps that for Semen Ormosiae Hosiei mud:
Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, 100-150 DEG C of autoclave, 20-40min sterilizings is placed on.
4. a kind of fermentation method according to claim 3 makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its It is characterised by, aspergillus oryzae is carried out into process activation before fermentation.
5. a kind of fermentation method according to claim 4 makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its It is characterised by, activates comprising the following steps that for aspergillus oryzae:Aspergillus oryzae is activated under conditions of 30-35 DEG C 40-50h.
6. a kind of fermentation method according to claim 5 makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its It is characterised by, the aspergillus oryzae after activation is diluted in the case of without miscellaneous bacteria and is sprayed on Semen Ormosiae Hosiei mud, 20-35 DEG C of fermentation 72h, Take 50-80 parts fermented product and mix with 120-180 part distilled water and stir evenly, 80-100 DEG C of heating 20-45min, cold filtration must contain red The solution of bean peptide, wherein, fermented product and distilled water are measured using parts by weight.
7. a kind of fermentation method according to claim 6 makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its It is characterised by, comprises the following steps that:
Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, pH value is adjusted to 6.0,121 DEG C of autoclave is placed on, 30min goes out Bacterium, water-bath be cooled to 30 DEG C it is standby, using PDA slant culture, aspergillus oryzae strain is taken into 3 inoculating loops and is inoculated in culture medium, 31 DEG C, it is inoculated in triangular flask after culture 48h and is enlarged culture, then the aspergillus oryzae for having activated is used into nothing in the case of without miscellaneous bacteria The lower spore of bacterium physiology salt washing, and its concentration is adjusted to into 108Individual/mL, sprays on Semen Ormosiae Hosiei mud, and spray flux is every 1000g Semen Ormosiae Hosiei mud sprays 3mL bacterium solutions, 30 DEG C of Jing fermentation 72h, takes 75 parts of fermented products and mixes with 150 parts of distilled water and stirs evenly, and 90 DEG C are heated 30min, cold filtration must contain the solution of Semen Phaseoli peptide, wherein, fermented product and distilled water are measured using parts by weight.
CN201611146609.5A 2016-12-13 2016-12-13 Method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using fermentation method Pending CN106578961A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201611146609.5A CN106578961A (en) 2016-12-13 2016-12-13 Method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using fermentation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201611146609.5A CN106578961A (en) 2016-12-13 2016-12-13 Method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using fermentation method

Publications (1)

Publication Number Publication Date
CN106578961A true CN106578961A (en) 2017-04-26

Family

ID=58801170

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201611146609.5A Pending CN106578961A (en) 2016-12-13 2016-12-13 Method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using fermentation method

Country Status (1)

Country Link
CN (1) CN106578961A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111067108A (en) * 2019-12-31 2020-04-28 北京农学院 Rose tartary buckwheat polypeptide oral liquid and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1786182A (en) * 2005-11-09 2006-06-14 天津科技大学 Method of preparing product containing sogabean peptide by microorganism fermentation process
CN101250575A (en) * 2008-03-07 2008-08-27 秋生堂生物科技(北京)有限公司 Method for preparing vanadium-containing peptides and products produced thereby
CN102470166A (en) * 2009-08-03 2012-05-23 株式会社钟化 Dipeptidyl peptidase-4 inhibitor
CN104293869A (en) * 2014-09-10 2015-01-21 内蒙古天奇生物科技有限公司 Bitter-free red bean polypeptide and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1786182A (en) * 2005-11-09 2006-06-14 天津科技大学 Method of preparing product containing sogabean peptide by microorganism fermentation process
CN101250575A (en) * 2008-03-07 2008-08-27 秋生堂生物科技(北京)有限公司 Method for preparing vanadium-containing peptides and products produced thereby
CN102470166A (en) * 2009-08-03 2012-05-23 株式会社钟化 Dipeptidyl peptidase-4 inhibitor
CN104293869A (en) * 2014-09-10 2015-01-21 内蒙古天奇生物科技有限公司 Bitter-free red bean polypeptide and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111067108A (en) * 2019-12-31 2020-04-28 北京农学院 Rose tartary buckwheat polypeptide oral liquid and preparation method thereof

Similar Documents

Publication Publication Date Title
CN102038046B (en) Production method of Pu'er tea ferment liquid, ferment powder and ferment beverage
CN102422934B (en) Method for preparing schizophyllumcommuneh fermented tea
CN108823261A (en) A kind of Ultra-low molecular weight Dendrobium officinale polysaccharide and its preparation and application
CN108244432A (en) A kind of fermentation Cordyceps militaris probiotic beverage and preparation method thereof
CN102408979B (en) Method for preparing wild papaya fruit vinegar and wild papaya fruit vinegar prepared by method
CN104531795A (en) Method for producing high-purity gamma-aminobutyric acid
CN110423658A (en) It is a kind of using sweet wormwood as the preparation method of the health liquor of major ingredient and health liquor
CN106720752A (en) Camellia chrysantha beverage and preparation method thereof
CN105124597A (en) Preparation method and application of functional monascus-fermented corn bran food
CN110339251B (en) Preparation method of fermented gardenia fermented soybean mycoplasm
CN102816667B (en) Ganoderma lucidum polysaccharide nutrition sweet wine and production process
CN106578961A (en) Method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using fermentation method
CN105482952A (en) Making method for folium artemisiae argyi and honey wine
CN106434116B (en) A kind of production technology of Lenlinus edodes red yeast rice rice wine
CN102703405A (en) Method for preparing lactase by taking bran as raw material
CN104164351B (en) A kind of liquid fermentation dividing method of apple vinegar
CN104509921A (en) Fructus momordicae beverage preparation method
CN105287848A (en) Fermented traditional Chinese medicinal preparation for treating newcastle disease
CN103525684B (en) Deep glossy ganoderma fermenting liquid prepares the method for Ganoderma vinegar
JPH11169135A (en) Medicinal ginseng processing sour liquid and its production
CN106167531B (en) A kind of method of enzyme process assisted extraction longan polysaccharide
CN109207304A (en) One kind, which is refreshed oneself, supports smart health-care rice wine and preparation method thereof
CN108893224A (en) A kind of preparation method of gynostemma pentaphylla red bayberry hydromel
CN107603810A (en) A kind of health liquor and preparation method thereof
CN106754030A (en) A kind of brewage process of auxotype glutinous rice sealed wine

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20170426

RJ01 Rejection of invention patent application after publication