CN106577731B - The antibacterial application of luminous energy transition-metal ions titanium - Google Patents
The antibacterial application of luminous energy transition-metal ions titanium Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
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Abstract
The present invention relates to anti-biotic materials, more particularly to a kind of antibacterial application of luminous energy transition-metal ions titanium, the luminous energy transition-metal ions titanium has broad-spectrum antibacterial action, the growth and breeding of the bacteriums such as Escherichia coli, Lou Che Shi streptomycete, bacterium Agrobacterium tumefaciems, pantoea agglomerans and Ralstonia solanacearum can effectively be inhibited, using luminous energy transition-metal ions titanium as broad-spectrum sterilization antibacterial agent be the equal of with green safe high-tech product substitute high poison high residue chemical pesticide, to ensure food safety, reduce environmental pollution, therefore can be widely applied in agricultural production.
Description
Technical field
The present invention relates to anti-biotic materials, and in particular to a kind of purposes of luminous energy transition-metal ions titanium antibiosis.
Background technique
China is large agricultural country, and the use of pesticide ensure that the yield and quality of crops.But as crops are caused a disease
The generation of bacterium drug resistance and largely apply the reasons such as the unification of chemical pesticide and Pesticide use, cause China's pesticide excessive
It is serious to apply phenomenon, and then causes soil, water pollution and food, Practice for Pesticide Residue in Agricultural Products excessive.Therefore, environment is developed and used
Friendly new pesticide is had very important significance with substituting harmful pesticide.
Titanium elements (Titanium, Ti) are widely distributed in nature, and content accounts for 0.64% in the earth's crust, organism
It is also widely present in interior, water body.More and more researches show that titanium can promote plant to propose the absorption and operating of some nutrients
The activity of a variety of enzymes in high plant improves the content of plant leaf blade Determination of Chlorophyll and improves the yield of crop, and can obviously change
Kind crop quality.But titanium elements usually with oxide, silicate reference state or ilmenite presence, therefore, it is difficult to for plant it is direct
It utilizes.
Currently, titanium paddy (Tianjin) Science and Technology Ltd. produces a kind of luminous energy transition-metal ions titanium (Titanium ion of
Variable valence with light energy, TIVL), titanium elements therein are to exist in the form of an ion, through studying
Show that luminous energy transition-metal ions titanium is soluble in water, is easy to plant absorption utilization.In fact, TIVL is in resistant to hydrolysis stabilizing ion titanium
It is improved and is obtained on the basis of the production technology of (Anti-hydrolyze Stabilized Ionic Titanium, ASIT)
The more stable product of the one kind obtained.Field experiment shows that spraying for the TIVL of low concentration has the advantages that many aspects: 1) improving
The germination percentage of vegetable seeds promotes root system of plant flourishing, and improves the yield and quality of crop；2) pesticide and chemical fertilizer are reduced
Usage amount.TIVL may be the characteristic for being titanium ion itself and there is energy level to jump over the reason of above effect, can be by luminous energy
It is transformed into chemical energy, then by enzymic transformation is biological energy source, to makes the gene of crop give full expression to, to promote plant growth.
But in terms of the disease specific prevention and treatment of crop, the effect in relation to luminous energy transition-metal ions titanium not yet has report at present
Road.
Goal of the invention
The object of the present invention is to provide a kind of antibacterial applications of luminous energy transition-metal ions titanium, with broad-spectrum antibacterial action.
Preferably, the antibacterial application is to inhibit Escherichia coli, Lou Che Shi streptomycete, Agrobacterium tumefaciems, pantoea agglomerans
And the growth and breeding of the bacteriums such as Ralstonia solanacearum.
The antibacterial new application of luminous energy transition-metal ions titanium disclosed by the invention provides safely and effectively novel for agricultural production
Broad-spectrum sterilization antibacterial agent.
Specific scheme is Escherichia coli, Lou Che Shi streptomycete, bacterium Agrobacterium tumefaciems, pantoea agglomerans and Solanaceae Lei Er
The bacteria suspension concentration of Salmonella is 2 × 103~104Under conditions of between CFU/mL, luminous energy transition-metal ions titanium is in each bacterium bacteria suspension
Effective Mlc is 4-16mg/L.
Preferably, luminous energy transition-metal ions titanium is 2 × 10 in concentration3~104Escherichia coli bacteria suspension between CFU/mL/at
Effective Mlc in the general bacterium bacteria suspension of group is 8-16mg/L.
Preferably, luminous energy transition-metal ions titanium is 2 × 10 in concentration3~104Lou Che Shi streptomycete bacterium between CFU/mL is outstanding
Effective Mlc in liquid/bacterium Agrobacterium tumefaciems bacteria suspension is 4-8mg/L.
Preferably, luminous energy transition-metal ions titanium is 2 × 10 in concentration3~104Ralstonia solanacearum bacteria suspension between CFU/mL
In effective Mlc be 16mg/L.
Crop securify production and environmental protection are country and common people's focus of attention, are concerning people's safety and health
Key factor.Therefore, high poison high residue chemical pesticide is substituted with green safe high-tech product, ensures food safety, reduces ring
Border pollution, this is the inexorable trend of development environment friendly agricultural.Applicant shows luminous energy transition-metal ions titanium through a large amount of experiment
Have to Escherichia coli, Lou Che Shi streptomycete, bacterium Agrobacterium tumefaciems pantoea agglomerans, Ralstonia solanacearum and significantly inhibits to make
With according to result we determined that luminous energy transition-metal ions titanium has broad-spectrum antibacterial effect.
The feature of five kinds of bacteriums such as above-described Escherichia coli, Lou Che Shi streptomycete is as follows:
Escherichia coli (Escherichia coli): belonging to Gram-negative bacteria, and rodlike brevibacterium is present in human body and animal
In enteron aisle.Most Escherichia coli be it is harmless, to maintain human body and animal intestinal tract balance play an important role, a small number of large intestines
Bacillus can lead to enterogastritis, urinary tract infections and neonatal meningitis, virulent type coli strain can lead to intestinal tissue necrosis and
Perforation.As the common bacterial strain in laboratory, Escherichia coli are type strain of the various substances of detection to bacterium antibacterial action.
Lou Che Shi streptomycete (Streptomyces rochei): streptomyces bacterium is that one obtained is separated from soil
Kind actinomyces.
Agrobacterium tumefaciems (Agrobacterium tumefaciens): crown gall pathogenic bacteria are to induce various plants
The pathogen of tumour, while being also the medium that genetic transformation is used in plant science research.
Pantoea agglomerans (Pantoe aagglomerans): belong to Gram-negative bacteria, body surface or endobiosis are in various plants
In, it is vegetative bacteria canker pathogenic bacteria, the especially main pathogenic bacteria of Poplar INA Bacterial Canker, is the weight of poplar development
Want restriction factor, which even infects human body, cause bloodstream infection, Soft Tissue Abscess: An Experimental, bone or the infection of joint, urinary tract infections,
The diseases such as peritonitis, serious person have pleural effusion, expiratory dyspnea, respiratory failure etc..
Ralstonia solanacearum (Ralstonia solanacearum): belonging to Gram-negative bacteria, be bacterial wilt pathogenic bacteria, can
The various crops such as tobacco, tomato, eggplant, ginger are infected, host range is extremely extensive, more than 40 sections totally 200 various crop can be endangered,
That a kind of crushing soil passes pathogenic bacteria, the bacterial wilt extent of injury of initiation is big, caused by economic loss in bacterial diseases of plants
In come out at the top.There is not strong measure to the prevention and treatment of bacterial wilt at present, based on enhancing field management, it is anti-to be aided with biology
Control that combine with chemical prevention be the most common comprehensive preventive health measures of bacterial wilt.
Detailed description of the invention
Fig. 1 is the upgrowth situation of Escherichia coli in the culture medium of the transition-metal ions titanium of luminous energy containing various concentration；
Fig. 2 is histogram of the various concentration luminous energy transition-metal ions titanium to the inhibiting effect of Escherichia coli；
Fig. 3 is the upgrowth situation of Lou Che Shi streptomycete in the culture medium of the transition-metal ions titanium of luminous energy containing various concentration；
Fig. 4 is histogram of the various concentration luminous energy transition-metal ions titanium to the inhibiting effect of Lou Che Shi streptomycete；
Fig. 5 is the upgrowth situation of Agrobacterium tumefaciems in the culture medium of the transition-metal ions titanium of luminous energy containing various concentration；
Fig. 6 is histogram of the various concentration luminous energy transition-metal ions titanium to the inhibiting effect of Agrobacterium tumefaciems；
Fig. 7 is the upgrowth situation of pantoea agglomerans in the culture medium of the transition-metal ions titanium of luminous energy containing various concentration；
Fig. 8 is histogram of the various concentration luminous energy transition-metal ions titanium to the inhibiting effect of pantoea agglomerans；
Fig. 9 is the upgrowth situation of Ralstonia solanacearum in the culture medium of the transition-metal ions titanium of luminous energy containing various concentration；
Figure 10 is histogram of the various concentration luminous energy transition-metal ions titanium to the inhibiting effect of Ralstonia solanacearum.
Specific embodiment
1-2 and attached drawing are further described technical solution disclosed by the invention with reference to embodiments.But these
Specific experiment and attached drawing are exemplary, and cannot treat them as limitation of the present invention.
Embodiment 1: the processing of strain
1, the preparation of strain
Strain: Escherichia coli (E.coli) bacterial strain JM109, Lou Che Shi streptomycete bacterial strain BK7 (S.rochei BK7), crown gall
Agrobacterium (A.tumefaciens) bacterial strain EHA105, these three bacterium are preserved in Chengdu Inst. of Biology, Chinese Academy of Sciences；It is agglomerating general
Bacterium (P.agglomerans), is isolated from Poplar INA Bacterial Canker, is managed by the Chinese Academy of Forestry's Microbiological Culture Collection
Center (China Forestry Culture Collection Center, CFCC) provides；Ralstonia solanacearum
(R.solanacearum), it is isolated from Cucumber wilt, is provided by Plant Protection institute, Chinese Academy of Agricultral Sciences.Described five
Kind of bacterium is saved using being preceding placed in 4 DEG C of refrigerator.
2, actication of culture
Escherichia coli, Lou Che Shi streptomycete and Agrobacterium tumefaciems are crossed respectively and are forwarded to corresponding LB solid medium
On, pantoea agglomerans, Ralstonia solanacearum are crossed respectively to be forwarded on corresponding nutrient culture medium, Escherichia coli and Lou Che Shi
Streptomycete is cultivated at 37 DEG C, and Agrobacterium tumefaciems, pantoea agglomerans and Ralstonia solanacearum are cultivated at 28 DEG C, grows to single colonie
It is spare；
Wherein, the formula of LB solid medium are as follows: tryptone 10.0g/L, yeast extract 5.0g/L, NaCl
10.0g/L, the pH of agar powder 15.0g/L, LB solid medium are 7.2；
The formula of nutrient culture medium are as follows: peptone 10.0g/L, beef extract 3.0g, NaCl 10.0g/L, agar powder
15.0g/L, the pH of nutrient culture medium are 7.0.
3, the preparation of bacteria suspension
1) each single colonie of picking is allowed to be uniformly dispersed respectively in 2mL aseptic deionized water with sample loading gun piping and druming, this is original
Liquid；
2) the EP centrifuge tube for choosing 10mL, is diluted to 10 for each stoste with aseptic deionized water with 10 times of relationship step by step4
Times, the bacterium solution of diluted graded concentrations is withed a hook at the end；
3) LB solid medium and nutrient culture medium, high pressure sterilization under conditions of 121 DEG C, 0.1MPa are prepared
20min is placed on super-clean bench and is cooled to 50 ± 2 DEG C, and inverted plate, solidification dries up condensed water, is respectively put into 10 into each culture medium
Sterilizing bead；
4) take the 100 μ L of bacterium solution of graded concentrations on LB solid medium respectively, bead rolls coating 2-3min to equal
It is even, bead is poured out, is sealed, constant temperature inversion under the conditions of 28 DEG C of the culture medium containing Escherichia coli and Lou Che Shi streptomycete was cultivated
Night, constant temperature under the conditions of 37 DEG C of the culture medium containing Agrobacterium tumefaciems, pantoea agglomerans and Ralstonia solanacearum are inverted overnight incubation, are remained
Remaining graded concentrations bacteria suspension is placed in 4 DEG C of refrigerators and saves backup, and observes bacterium colony upgrowth situation, chooses suitable bacterial concentration ladder
Degree.
According to flat-plate bacterial colony growing state, when determining that diluted concentration is 100-1000 times after 5 kinds of bacterium picking single colonies, bacterium colony
Essentially single colonie and medium density, i.e., the bacteria suspension concentration of preferably 5 kinds bacterium is 2 × 103~104Between CFU/mL, embodiment 2
The bacteria suspension concentration of selection is tested i.e. in the concentration range.
Embodiment 2: the antibacterial experiment of ion titanium
1, the preparation of anti-biotic material
Anti-biotic material: luminous energy transition-metal ions titanium (Titanium ion of variable valence with light
Energy, TIVL), abbreviation ion titanium is produced by titanium paddy (Tianjin) Science and Technology Ltd., from titanium paddy (Tianjin) Science and Technology Ltd.
And its agency " Liangshan Mountain Tai Tu agricultural science and technology Co., Ltd " can directly buy.Other metallic elements are not added with, ion titanium is female
Liquid concentration is 4mg/mL.Actual production lot number used in the present embodiment is 2016.10-002.
Applicant understands from titanium paddy (Tianjin) Science and Technology Ltd. and knows, luminous energy transition-metal ions titanium used in the present invention is practical
On exactly entitled " a kind of luminous energy transition-metal ions liquid titanium compound and preparation method thereof " (application No. is 201510773354.4)
Titanium ion chelate solution disclosed in patent of invention, agents useful for same of the present invention be titanium paddy (Tianjin) Science and Technology Ltd. according to
The product of the production of method disclosed in the patent of invention, i.e., be diluted to titaniferous amount of element with pure water for titanium ion chelate solution
For the solution (i.e. the ion titanium mother liquid concentration of luminous energy transition-metal ions titanium is 4mg/mL) of 4g/L.The present embodiment reagent and patent of invention
Luminous energy transition-metal ions liquid titanium compound compare, luminous energy transition-metal ions titanium of the present invention is the equal of a kind of sterling, i.e., wherein
Other metallic elements such as zinc, iron, manganese are not added.
2, the preparation of plate
With 150mL triangular flask, LB solid medium and nutrient culture are prepared respectively according to the formula that embodiment 1 is recorded
Base, 60mL/ bottles, high pressure sterilization is cooled to 50 ± 2 DEG C, add respectively mother liquid concentration be 0 μ L of ion titanium of 4mg/mL, 60 μ L,
120 μ L, 240 μ L, making the final concentration of each culture medium intermediate ion titanium is not respectively 0mg/L, 4mg/L, 8mg/L, 16mg/L, not plus ion
The culture medium of titanium (final concentration of 0mg/L) be reference substance, shake up, inverted plate, every 60mL culture medium fall 3 plates (i.e. every kind from
The culture medium of sub- titanium concentration has the plate as 3), solidification dries up condensed water, is respectively put into 10 sterilizing beades.
3, anti-biotic material is added
1) concentration retained in 1 step 4) of embodiment is chosen 2 × 103~104Each bacterium bacteria suspension between CFU/mL, often
Kind bacterium is blown and beaten respectively with sample loading gun mixes and is packed as 4 parts, every part of 1mL, is separately added into the ion titanium that mother liquid concentration is 4mg/mL
0mL, 1mL, 2mL and 4mL, making bacterium intermediate ion titanium final concentration is 0mg/L, 4mg/L, 8mg/L, 16mg/L；
2) it will be blown and beaten and be mixed added with the bacteria suspension of ion titanium with the sample loading gun of 200 μ L, and take 100 μ L bacteria suspensions to containing respectively
On the suitable plate of respective concentration ion titanium (i.e. step 2 be prepared plate), plate is rocked, bead rolling is applied to
It is even.It should be noted that the bacteria suspension of Escherichia coli and Lou Che Shi streptomycete is the LB solid medium for being coated on respective concentration
On plate, Agrobacterium tumefaciems, pantoea agglomerans and Ralstonia solanacearum are the nutrient cultures for being coated on respective concentration
On the plate that base falls.
3) Escherichia coli and Lou Che Shi streptomycete are inverted culture under the conditions of temperature is 37 DEG C, Agrobacterium tumefaciems, agglomerating general
Bacterium and Ralstonia solanacearum are inverted culture under conditions of temperature is 28 DEG C, observe statistics after 24-120h.
4, bacterium colony counting and variance analysis
The clump count in each plate is counted, the fungistatic effect of ion titanium is analyzed.Mean ± SD the expression of flat-plate bacterial colony number, from
Sub- titanium indicates the fungistatic effect of 5 kinds of bacterium with bacteriostasis rate, bacteriostasis rate=((control group average colony number-experimental group average colony
Number)/control group average colony number) × 100%.With t check analysis group difference, P < 0.05 is considered as significant difference, P < 0.01
It is extremely significant to be considered as difference.
1) antibacterial action of the ion titanium to Escherichia coli
As shown in Figs. 1-2, observation coli strain JM109 bacterium colony is cultivated in the culture medium of the titanium of ion containing various concentration
For 24 hours, the growing state of 48h, 72h, as the result is shown: when culture medium intermediate ion titanium concentration is 4mg/L, having to Escherichia coli certain
Inhibiting effect, but it is not significant；When culture medium intermediate ion titanium concentration is 8mg/L, almost without bacterium colony growth, compared with the control, resist
Bacterium effect is extremely significant；After culture for 24 hours, control group average colony number is 470 ± 19, and ion titanium concentration is the culture of 8mg/L
Average colony number is 4 ± 1 in base, and up to 99.15%, and as incubation time extends to 72h, ion titanium concentration is bacteriostasis rate
There is no new bacterium colony to grow in the culture medium of 8mg/L, shows that concentration is that the ion titanium of 8mg/L can effectively inhibit Escherichia coli
Growth, specifically combine shown in Fig. 1-2 and table 1；To the fungistatic effect and concentration of Escherichia coli when ion titanium concentration is 16mg/L
For the essentially identical of 8mg/L, therefore the concrete outcome that ion titanium concentration is 16mg/L is not included in chart；T check analysis table
Bright, compared with, the difference of each ion titanium experimental group and control group is extremely significant, P < 0.01 * *.
2) antibacterial action of the ion titanium to Lou Che Shi streptomycete
As shown in Figure 3-4, observation Lou Che Shi streptomycete bacterial strain BK7 bacterium colony is trained in the culture medium of the titanium of ion containing various concentration
Support for 24 hours, the growing state of 48h, 72h, as the result is shown: culture for 24 hours after, that is, observe apparent difference, be in ion titanium concentration
The growth of Lou Che Shi streptomycete is significantly inhibited when 4mg/L, and control group average colony number is 1050 ± 153, and ion titanium is dense
Degree is respectively that the clump count in the culture medium of 4mg/L, 8mg/L is respectively 28 ± 3 and 20 ± 1, and bacteriostasis rate is respectively
97.33% and 98.10%；With the extension of incubation time, although ion titanium concentration is respectively in the culture medium of 4mg/L, 8mg/L
Bacterium colony is grown up, but rare new bacterium colony is grown；After cultivating 72h, there is a little new bacterium in the culture medium that ion titanium concentration is 4mg/L
It falls and grows, and ion titanium concentration grows for the still not new bacterium colony of the plate of 8mg/L and (is detailed in Fig. 3-4 and table 1)；Ion titanium is dense
It is the essentially identical consistent of 8mg/L to the fungistatic effect of Lou Che Shi streptomycete and concentration when degree is 16mg/L, therefore ion titanium is dense
Degree is that the concrete outcome of 16mg/L is not included in chart；T check analysis shows the difference pole of ion titanium experimental group and control group
Significantly, P < 0.01 * *, but experimental group of the ion titanium concentration between 4-8mg/L and control group do not have notable difference, illustrate ion
Titanium concentration is the growth that 4mg/L can effectively inhibit Lou Che Shi streptomycete.
3) antibacterial action of the ion titanium to Agrobacterium tumefaciems
It because Agrobacterium tumefaciems growth is slower, therefore is counted after cultivating 48h, as seen in figs. 5-6, observes Agrobacterium tumefaciens strain
EHA105 bacterium colony cultivates the growing state of 48h, 72h in the culture medium of the titanium of ion containing various concentration, as a result with Lou Che Shi strepto-
Mushroom is seemingly: after culture 48h, control group average colony number is 286 ± 15, and ion titanium concentration is respectively the training of 4mg/L, 8mg/L
Supporting in base clump count in clump count is respectively 84 ± 9 and 31 ± 3, and bacteriostasis rate is respectively 70.63% and 89.16, %, i.e., from
Fungistatic effect is more preferable when sub- titanium concentration is 8mg/L；When ion titanium concentration is 16mg/L to the fungistatic effect of Agrobacterium tumefaciems with it is dense
Degree is the essentially identical consistent of 8mg/L, therefore the concrete outcome that ion titanium concentration is 16mg/L is not included in chart；T is examined
Analysis shows ion titanium experimental group and the difference of control group are extremely significant, P < 0.01 * *.
4) antibacterial action of the ion titanium to pantoea agglomerans
As Figure 7-8, observation pantoea agglomerans bacterium colony cultivated in the culture medium of the titanium of ion containing various concentration 48h, 72h,
The growing state of 96h, as the result is shown: after culture 48h, control group average colony number is 211 ± 11, the concentration of titanium containing different ions
Culture medium in clump count difference it is obvious, specifically, ion titanium concentration is respectively in the culture medium of 4mg/L, 8mg/L, 16mg/L
Clump count is respectively 91 ± 5,49 ± 3 and 26 ± 2, and bacteriostasis rate is respectively 56.87%, 76.78%, 87.68%；It is training
After supporting 72h and 96h, the bacterium colony that each plate has only a few new is grown, to the antibacterial of pantoea agglomerans when ion titanium concentration is 16mg/L
Effect is preferably (being detailed in Fig. 7-8 and table 1)；T check analysis shows that the difference of ion titanium experimental group and control group is extremely significant, * * P <
0.01。
5) antibacterial action of the ion titanium to Ralstonia solanacearum
As shown in figs. 9-10, observation Ralstonia solanacearum bacterium colony cultivated in the culture medium of the titanium of ion containing various concentration 72h,
The growing state of 96h, 120h, as the result is shown: after culture 72h, control group average colony number is 320 ± 15, and ion titanium is dense
Degree is that 4mg/L and 8mg/L has certain inhibiting effect to bacterium colony growth, and average colony number is respectively 245 ± 11 and 214
± 9, bacteriostasis rate is respectively 23.44% and 33.13%, and compared with the control group, the fungistatic effect of both concentration ion titaniums is poor
Different significant (P < 0.05)；After cultivating 72h, ion titanium concentration is clump count 83 ± 5 in the culture medium of 16mg/L, and bacteriostasis rate is
74.06%, compared with the control group, fungistatic effect difference is extremely significant (P < 0.01), shows that the ion titanium that concentration is 16mg/L can
Effectively inhibit the growth of Ralstonia solanacearum；After cultivating 96h and 120h, each plate has a small number of new bacterium colonies to grow, overall
The ion titanium that concentration is 16mg/L for upper is most obvious ((being detailed in Fig. 9-10 and table 1)) to the antibacterial action of Ralstonia solanacearum.
By the above experimental result it is found that different bacterium is inconsistent to the susceptibility of ion titanium, but exist in ion titanium concentration
The growth of five kinds of bacteriums such as Escherichia coli, Lou Che Shi streptomycete can be significantly limited within the scope of 16mg/L, as shown in table 1.
Influence of the 1 various concentration luminous energy transition-metal ions titanium of table to 5 kinds of bacteriums
Note: * significant difference (P < 0.05), * * difference are extremely significant (P < 0.01).
In conclusion luminous energy transition-metal ions titanium has broad-spectrum antibacterial effect, killed using luminous energy transition-metal ions titanium as wide spectrum
Bacterium antibacterial agent is the equal of substituting high poison high residue chemical pesticide with green safe high-tech product, therefore can be in agricultural production
In be widely used on crop, reduce the disease incidence and occurring degree of crop.
Claims (6)
1. a kind of antibacterial application of luminous energy transition-metal ions titanium, it is characterised in that: the antibacterial application of luminous energy transition-metal ions titanium has wide
Compose antibacterial action.
2. the antibacterial application of luminous energy transition-metal ions titanium according to claim 1, it is characterised in that: the antibacterial application is
Inhibit the growth and breeding of Escherichia coli, Lou Che Shi streptomycete, bacterium Agrobacterium tumefaciems, pantoea agglomerans and Ralstonia solanacearum.
3. the antibacterial application of luminous energy transition-metal ions titanium according to claim 1, it is characterised in that: the bacteria suspension to antibacterial is dense
Degree is 2 × 103~104Under conditions of between CFU/mL, effective Mlc of the luminous energy transition-metal ions titanium in each bacterium bacteria suspension is
4-16mg/L。
4. the antibacterial application of luminous energy transition-metal ions titanium according to claim 1, it is characterised in that: luminous energy transition-metal ions titanium exists
Concentration is 2 × 103~104Effective Mlc in Escherichia coli bacteria suspension/pantoea agglomerans bacteria suspension between CFU/mL is 8-
16mg/L。
5. the antibacterial application of luminous energy transition-metal ions titanium according to claim 1, it is characterised in that: luminous energy transition-metal ions titanium exists
Concentration is 2 × 103~104It is effective antibacterial in Lou Che Shi streptomycete bacteria suspension/bacterium Agrobacterium tumefaciems bacteria suspension between CFU/ml
Concentration is 4-8mg/L.
6. the antibacterial application of luminous energy transition-metal ions titanium according to claim 1, it is characterised in that: luminous energy transition-metal ions titanium exists
Concentration is 2 × 103~104Effective Mlc in Ralstonia solanacearum bacteria suspension between CFU/mL is 16mg/L.
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| Synthesis, characterization and biochemical studies of some mixed ligand complexes of Ti(IV) ion complexes;Sharma, R. N.;《Journal Chemtracks》;20151231;第133-136页 * |
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