CN1065691A - Method for removing blood stain and yellow spot of silkworm cocoon (or silk) - Google Patents
Method for removing blood stain and yellow spot of silkworm cocoon (or silk) Download PDFInfo
- Publication number
- CN1065691A CN1065691A CN 91107176 CN91107176A CN1065691A CN 1065691 A CN1065691 A CN 1065691A CN 91107176 CN91107176 CN 91107176 CN 91107176 A CN91107176 A CN 91107176A CN 1065691 A CN1065691 A CN 1065691A
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- CN
- China
- Prior art keywords
- silk
- acid
- washing
- cocoon
- time
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000008280 blood Substances 0.000 title claims abstract description 13
- 210000004369 blood Anatomy 0.000 title claims abstract description 13
- 238000000034 method Methods 0.000 title claims abstract description 11
- 241000255789 Bombyx mori Species 0.000 title abstract description 8
- 238000005406 washing Methods 0.000 claims abstract description 8
- 150000001413 amino acids Chemical class 0.000 claims abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 230000007062 hydrolysis Effects 0.000 claims description 7
- 238000006460 hydrolysis reaction Methods 0.000 claims description 7
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 claims description 6
- 238000007654 immersion Methods 0.000 claims description 6
- 210000002189 macula lutea Anatomy 0.000 claims description 6
- 230000007935 neutral effect Effects 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 4
- 239000004094 surface-active agent Substances 0.000 claims description 4
- 238000005342 ion exchange Methods 0.000 claims description 2
- 238000005185 salting out Methods 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 238000005903 acid hydrolysis reaction Methods 0.000 claims 3
- 239000003344 environmental pollutant Substances 0.000 abstract description 3
- 231100000719 pollutant Toxicity 0.000 abstract description 3
- 238000002791 soaking Methods 0.000 abstract 2
- 239000000413 hydrolysate Substances 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 8
- 239000007864 aqueous solution Substances 0.000 description 7
- 108091005804 Peptidases Proteins 0.000 description 4
- 239000004365 Protease Substances 0.000 description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 150000002148 esters Chemical class 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 125000005233 alkylalcohol group Chemical group 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 229920000191 poly(N-vinyl pyrrolidone) Polymers 0.000 description 1
- -1 polyoxyethylene Polymers 0.000 description 1
- 229940051841 polyoxyethylene ether Drugs 0.000 description 1
- 229920000056 polyoxyethylene ether Polymers 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
Landscapes
- Chemical Or Physical Treatment Of Fibers (AREA)
Abstract
The invention belongs to a method for removing blood stain and yellow spots of silkworm cocoons (or silks), which is characterized in that the blood stain or yellow spot silkworm cocoons or silks are subjected to the procedures of soaking, hydrolyzing, washing and the like under proper conditions, and pollutants are thoroughly removed on the premise of ensuring the strength and the quality of the silks to be unchanged, so that the silk can be reeled to obtain certified silks, and the yield is improved by 3-6 times. More than ten kinds of amino acids can be recovered from the soaking solution and the hydrolysate. And the method is simple and is suitable for popularization and application in silk factories, silkworm cocoon purchasing departments or silk companies.
Description
The invention belongs to blood stains or macula lutea etc. outer silk cocoon (or silk) remove the method that pollutant becomes certified products silk cocoon (or silk).
Silk cocoon can damage by pressure lacking the part silkworm chrysalis in production, storage and transport process inevitably, and the body fluid of pupa oozes out on silk cocoon, forms blood stains.Last excreta also might fall below it earlier (or) and does on the silk cocoon of cocoon before silkworms spin silk, forms macula lutea, and these pollutants bond mutually with between the silk in the cocoon and the silk, make it to be used for filature, can only the much lower long Tuhe recotti of productive value.
Task of the present invention is: in the intensity that guarantees silk, under the prerequisite that performance is not affected, with gentle way, with the dirt on the cocoon, thoroughly remove, make it can reel out the certified products the same with common silk cocoon (good) silk.
In order to realize removing the purpose of dirt, the present invention proposes following technical solution, and the first step is carried out sorting with blood cocoon and yellow spotted cocoon.In second step, immersion is soaked 10～40h with the yellow silk cocoon of blood stains (or silk) in following solution, and this solution includes 2～10% neutral salt, 0～0.1% CHCl
3With the surfactant of 0.01～0.29% 18＞HLB＞13, wherein, neutral salt comprises NaCl, Na
2SO
4In one or more, surfactant comprises the rare ester of poly alkyl alcohol oxygen second, alkane phenolic group polyoxyethylene ether, fatty alcohol sulphuric acid receive or the emulsifying agent of other 13＞HLB＜16 in one or more.
The 3rd step: pull silk cocoon (or silk) washing one out to secondary.
The 4th step: enzyme hydrolysis be 30～50 ℃ with the cocoon (or silk) through washing in temperature, and concentration is hydrolysis 2～50h in the aqueous solution of the neutral salt of protease, 10～60g/l of 10～120 units/ml and 0.01～0.3% surfactant.
The 5th step will be in the aqueous solution of 0～15ml/l in acid content through the silk cocoon (or silk) of enzyme hydrolysis, hydrolysis 2～48 hours, and the kind of acid can be C
nH
2n+1COOH(n is 0,1,2), glycolic acid, CH
mCl
3-mCOOH(m is 0,1,2,3) in the sulfuric acid, hydrochloric acid one or more.
The 6th step: be washed to PH6～7.
Now in conjunction with the embodiments this is further described invention.
Embodiment 1, the first step: sorting blood cocoon and yellow spotted cocoon, so that handle respectively.
Second step: the aqueous solution that is mixed with following concentration: 2～10% NaCl, 0～3% CHCl
3, 0.5% phenolic group polyoxyethylene ester in the ninth of the ten Heavenly Stems is soaked 10～30h with the blood stains silk cocoon in this solution.
The 3rd step:, again this batch silk cocoon is put into that 30～40 ℃ of temperature degree contain the NaCl of 20～30g/l and the aqueous solution of protease of 10～115 units/ml soaks 8～18h the 4th step with flushing with clean water 1～2 time.
The 5th step: stirring under the companion, adding H by every premium on currency solution
2SO
4, 5～10ml, hydrolysis 6～40h,
In the 6th step, pull silk cocoon out and be washed to PH6～7.0, filature routinely.
Embodiment 2, the first step, and sorting blood cocoon and yellow spotted cocoon are so that handle respectively.Second step: the aqueous solution of preparing following concentration: 3～5% Na
2SO
4, 0.07% CHCl
3, 0.10% ester fat alcohol sodium sulphate, stir evenly, yellow silkworm cocoon is soaked 15～20h inside, the 3rd step was pulled washing one out to secondary.The 4th step: prepare the following aqueous solution, 10～112 units/ml protease, the Na of 30g/l
2SO
4, 5～10h will be soaked through the yellow cocoon of above-mentioned processing in temperature adjustment to 34～36 ℃ in this solution.The 5th step added glycolic acid 7ml/l and stirs evenly in this solution, behind 10～18h, pull silk cocoon out
The 6th step was washed to PH6.5～7, and at this moment the macula lutea of silkworm cocoon removes, but the more solito filature.
Embodiment 3, and the aqueous solution that will have yellow silk to put into the peregal of 3～5% NaCl and 0.1% soaks 6～40h taking-up, washes 1～2 time, it is put into the Na of 5～90,000 units/l protease and 3～5% again
2SO
4In 35～40 ℃ of constant temperature 40h, take out out and wash 1～2 time in the solution, be the certified products silk.
Contain a large amount of amino acid in immersion liquid and the hydrolyzate, as ASP, Asn, Cyz, Glu, Gly, His, Leu, Lys, Ser, Tnr, Tyr, Val ... available accent PI or ion exchange column also or salting out method reclaimed and purified.
Claims (7)
1, a kind of silk cocoon (or silk) blood stains macula lutea is removed method, it is characterized in that: blood stains macula lutea silk cocoon (or silk) is washed successively enzyme hydrolysis, acid hydrolysis, operations such as washing for the second time through immersion, the first time.
2,, it is characterized by and contain 2～10% neutral salt, 0～0.1% CHCl in this liquid by the described immersion liquid of claim 1
3With 18＞HLB＞13, consumption is 0.01～0.2% any kind of surfactant, and wherein, neutral salt comprises NaCl, Na
2SO
4In one or more.
3, wash requirement for washing immersion liquid off the described first time by claim 1.
4, by the described enzyme hydrolysis of claim 1, it is characterized in that every premium on currency solution inner protein enzyme 1～120,000 unit, neutral salt 1～60g, and soak 2～48h at 30～50 ℃ of constant temperature.
5, by the described acid hydrolysis of claim 1, it is characterized in that every premium on currency solution contains acid 0～15ml, the kind of acid can be C
nH
2n+1COOH(n is 0,1,2), glycolic acid, CH
mCl
3-mCOOH(m is 0,1,2,3) sulfuric acid, hydrochloric acid ... in one or more, the acid hydrolysis time is 2～48h.
6, by the described washing second time of claim 1, it is characterized in that washing with water to PH6～7.
7, the amino acid in immersion liquid and the hydrolyzate, available accent PI method, ion-exchange or salting out method are reclaimed and are purified.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91107176 CN1065691A (en) | 1991-04-11 | 1991-04-11 | Method for removing blood stain and yellow spot of silkworm cocoon (or silk) |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91107176 CN1065691A (en) | 1991-04-11 | 1991-04-11 | Method for removing blood stain and yellow spot of silkworm cocoon (or silk) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1065691A true CN1065691A (en) | 1992-10-28 |
Family
ID=4908533
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 91107176 Pending CN1065691A (en) | 1991-04-11 | 1991-04-11 | Method for removing blood stain and yellow spot of silkworm cocoon (or silk) |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1065691A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109234814A (en) * | 2018-07-23 | 2019-01-18 | 安徽三利丝绸集团有限公司 | It is a kind of to guarantee that the two of cocoon quality rushes drying means |
-
1991
- 1991-04-11 CN CN 91107176 patent/CN1065691A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109234814A (en) * | 2018-07-23 | 2019-01-18 | 安徽三利丝绸集团有限公司 | It is a kind of to guarantee that the two of cocoon quality rushes drying means |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |