CN106554918A - A kind of microorganism cultivates integration apparatus - Google Patents
A kind of microorganism cultivates integration apparatus Download PDFInfo
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- CN106554918A CN106554918A CN201610313017.1A CN201610313017A CN106554918A CN 106554918 A CN106554918 A CN 106554918A CN 201610313017 A CN201610313017 A CN 201610313017A CN 106554918 A CN106554918 A CN 106554918A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12M31/00—Means for providing, directing, scattering or concentrating light
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention discloses a kind of microorganism cultivates integration apparatus, including chamber and the constant temperature gas bath circulating generator being arranged in chamber, air cleaner, ozone generator, uviol lamp, operation table apparatus and oscillation device and control system etc..Operation table apparatus are movably set in chamber and for carrying out inoculation operation, launch to form the platform being suspended from chamber under first state, and the side wall for being laminated on the chamber is received under the second state；Oscillation device is moved repeatedly for driving the blake bottle of microbe inoculation to carry out level.Control system is used to control above-mentioned each device action to realize that automatic intelligent regulates and controls.The integration apparatus of the present invention can carry out various operations, it is ensured that the continuity of microbial cultivation process, it is to avoid microbiological contamination, improve the success rate of microorganism culture.
Description
Technical field
The present invention relates to a kind of equipment, more particularly to a kind of microorganism culture integration apparatus.
Background technology
Microorganism culture has great importance in the field such as biology, medicine, food, sewage disposal, is most basic modal technological means in the operating process such as microbiological research, bio-pharmaceuticals, food inspection, water quality detection.The process complexity of microorganism culture is loaded down with trivial details, generally requires step-by-step processing, and the required equipment for using is more and needs to change between distinct device.Due in microbial cultivation process to temperature, humidity, cleanliness factor etc. require it is very harsh, between different devices change when easily microbiological contamination and cause culture failure.In addition, plurality of devices does not only take up a large amount of spaces, also need to operating personnel skillfully using each equipment and timing these equipment are carried out maintaining, are keeped in repair, spend substantial amounts of human and material resources and financial resources, also bring greatly using difficult to user, thus, it is the active demand of industry development by equipment integration, intellectuality.
The content of the invention
The invention provides a kind of microorganism cultivates integration apparatus, the weak point existing for the microbial cultivation equipment of prior art is which overcomed.
The technical solution adopted for the present invention to solve the technical problems is：A kind of microorganism cultivates integration apparatus, including：
Chamber, the chamber are provided with Removable Form；Constant temperature gas bath circulating generator, is arranged at the chamber bottom, for producing constant temperature circulating air-flow to remain adapted to the temperature of microorganism culture in chamber；Air cleaner, is arranged at the top of the chamber, for filtering into the constant temperature circulating air-flow in chamber；Ozone generator, is arranged at the chamber bottom, and for producing ozone, the ozone of generation is in constant temperature circulating air-flow carrying out ozone dissipation to chamber；Uviol lamp, is arranged at the top of the chamber, for carrying out ultraviolet dissipation to chamber；Operation table apparatus, are movably set in chamber and for carrying out inoculation operation, and the operating desk is installed under a first state and launches to form the platform being suspended from chamber, and the side wall for being laminated on the chamber is received under one second state；Oscillation device, is arranged in chamber, and the oscillation device is provided with some fixed seats for placing blake bottle, is moved with driving the blake bottle of microbe inoculation to carry out level repeatedly；Control system, for controlling constant temperature gas bath circulating generator, air cleaner, ozone generator, uviol lamp and the oscillation device action；
Microorganism culture is carried out using the equipment to comprise the following steps：Launch operation table apparatus in chamber and place equipment needed for microbial inoculant；Removable Form is closed, the constant temperature gas bath circulating generator is started successively by control system and is produced that constant temperature circulating air-flow, the air cleaner carry out filtering to constant temperature circulating air-flow, ozone generator carries out ozone dissipation；Ozone generator is closed, the blake bottle equipped with culture medium is put into after 2-3 hours and Removable Form is closed, starting uviol lamp carries out dissipation；Dissipation terminates to be inoculated with chamber, and operation table apparatus are packed up after inoculation, and postvaccinal blake bottle is placed in the fixed seat of oscillation device, controls oscillation device by control system and is vibrated.
Preferably, the wall body of the chamber is made up of corrosion resistant plate, and the Removable Form is the slide type glass forms that can adjust open degree up and down.
Preferably, it is described operation table apparatus include an operation panel and two adjustable shelfs, the operation panel side is rotationally connected with the wall of chamber side, two adjustable shelfs be hingedly connected to operation panel with respect to opposite side two ends and chamber side wall between；The adjustable shelf includes two connecting rods for rotating connection, and two connecting rod is turned in 180 ° so that the operation panel horizontal development in the first state, and two connecting rod turns to overlap so that the operation panel receives folded laminating chamber side wall in this second state.
Preferably, the buckle being mutually matched is provided with the operation panel opposite side and correspondence chamber side wall, operation panel is mutually fixed with chamber side wall by buckle in the second state.
Preferably, the operation table apparatus include an operation panel and two poles, and the operation panel side is rotationally connected with the wall of chamber side, and two poles can be articulated with the chamber side wall below operation panel in which horizontally rotate；Two pole turns to and operation panel vertical with chamber side wall and is set up on pole in the first state, and two pole turns to laminating chamber side wall, the sagging chamber side wall of fitting of the operation panel in this second state.
Preferably, the oscillation device includes carrying platform and for driving carrying platform to do the drive mechanism of horizontal reciprocating movement, and on carrying platform, the drive mechanism connects and is controlled by the control system fixed seat.
Preferably, the carrying platform offers some through holes.
Preferably, the control system includes main control module, power module and input module；The instruction of main control module receives input module input simultaneously accordingly controls constant temperature gas bath circulating generator, air cleaner, ozone generator, uviol lamp and oscillation device action；Power module is used to power.
Preferably, the main control module is micro-control unit, and the input module is touch screen.
Preferably, fluorescent lamp is additionally provided with the chamber.
Compared to prior art, the invention has the advantages that：
First, constant temperature gas bath circulating generator, air cleaner, ozone generator and uviol lamp etc. are provided with the chamber of the integration apparatus of the present invention, for the sterilization that carries out disinfection, the environment of dustless, the aseptic ultra-clean and constant temperature of maintenance in chamber is made；Oscillation device and mobilizable operation table apparatus are provided with chamber, operation table apparatus launch to be convenient for the operation such as inoculation, stacked to be received on the wall of side when shaken cultivation is subsequently carried out, and are not take up space, postvaccinal blake bottle can be placed directly within oscillation device, be prevented effectively from microbiological contamination；The integration apparatus of the present invention can carry out various operations, it is ensured that the continuity of microbial cultivation process, improve the success rate of microorganism culture.
Which two, by the work of the units such as control system control cabinet constant indoor temperature gas bath circulating generator, oscillation device, and connect main control unit to be operated by touch screen, the parameters such as temperature, the duration of the programs such as dissipation, vibration can be set according to the habit of specified microorganisms, intelligence degree is high, can automatic regulation, it is easy to use.
Third, integration apparatus reduce multiple equipment taking up room, simplifying operating personnel needs the number of devices of operation, reduces manual work's amount, is suitable to popularization and application.
The present invention is described in further detail below in conjunction with drawings and Examples；But a kind of microorganism culture integration apparatus of the present invention are not limited to embodiment.
Description of the drawings
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the structural representation (side-looking) under one embodiment of the invention operation table apparatus first state；
Fig. 3 is Fig. 2 operation table apparatus structural representations (side-looking) in the second condition；
Fig. 4 is the structural representation (side-looking) under another embodiment of the present invention operation table apparatus first state；
Fig. 5 is the front view that Fig. 4 operates table apparatus；
Fig. 6 is the structural representation (side-looking) under Fig. 4 operation the second states of table apparatus；
Fig. 7 is the circuit module schematic diagram of the present invention.
Specific embodiment
Embodiment, it is shown in Figure 1, a kind of microorganism culture integration apparatus of the present invention include a chamber 1, the wall body of chamber 1 is made up of corrosion resistant plate and is provided with moveable forms, the forms are the slide type glass forms structures that can adjust open degree up and down, it is excessive without regard to opening to the height requirement that can meet operation in order to adjust, in order to avoid the cleanliness factor inside destruction chamber.The bottom of chamber 1 is provided with constant temperature gas bath circulating generator 2 and ozone generator 3, and top is provided with air cleaner 4 and uviol lamp 5.Constant temperature gas bath circulating generator 2 is for producing constant temperature circulating air-flow to remain adapted to the temperature of microorganism culture in chamber 1；Air cleaner 4 is for filtering into the constant temperature circulating air-flow in chamber 1；Ozone generator 3 is used to produce ozone, and the ozone of generation is in constant temperature circulating air-flow carrying out ozone dissipation to chamber 1；Uviol lamp 5 is for carrying out ultraviolet dissipation to chamber 1.By said apparatus, make in chamber, to maintain the environment of ultra-clean and constant temperature.
Operation table apparatus 6 and oscillation device 7 are provided with chamber.Operation table apparatus 6 are movably set in chamber 1 and for carrying out inoculation operation, and the operation table apparatus 6 launch to form the platform being suspended from chamber 1 under a first state, and the side wall for being laminated on chamber 1 is received under one second state.Oscillation device 7 is moved repeatedly for driving the blake bottle of microbe inoculation to carry out level.
In the present embodiment, referring to figs. 2 to Fig. 4, operation table apparatus 6 include an operation panel 61 and two adjustable shelfs 62, and the side of operation panel 61 is rotationally connected with 1 side wall of chamber to be overturn relative to 1 side wall of chamber；Two adjustable shelfs 62 are hingedly connected between the 1 side wall of two ends and chamber of 1 relative opposite side of operation panel.Specifically, adjustable shelf 62 includes two connecting rods for rotating connection, two connecting rods are turned in 180 ° so as to 61 horizontal development of operation panel be hung in top by adjustable shelf 62 in the first state, a triangular structure of right angle, and adjustable shelf 62 are now formed between operation panel 61, adjustable shelf 62 and chamber side wall as the hypotenuse of right angled triangle.61 horizontal development of operation panel forms operating desk, in order to place the equipment such as the required alcolhol burner of inoculation, lighter, alcohol watering can, oese, cotton ball soaked in alcohol, liquid-transfering gun and pipette tips box, and the action that can be inoculated with.After the completion of inoculation, inoculation equipment is taken out, two connecting rods are turned to and overlap and fit in 1 side wall of chamber in the second condition, you can the vertical receipts of operation panel 61 is folded with 1 side wall of chamber of fitting.The buckle (not shown) being mutually matched is provided with 61 opposite side of operation panel and correspondence 1 side wall of chamber, operation panel 61 is vertically received poststack in the second state and mutually fixed with chamber side wall by buckle, you can concede the space in chamber 1.
In another embodiment, with reference to Fig. 5 to Fig. 6, operation table apparatus 6 include an operation panel 61 and two poles 63, and the side of operation panel 61 is rotationally connected with 1 side wall of chamber to be overturn relative to 1 side wall of chamber；Two poles 63 can be articulated with the 1 side wall of chamber below operation panel 61 in which horizontally rotate.Operation panel 61 is lifted in the first state, two poles 63 are turned to vertical with chamber side wall, operation panel 61 both can level frame be located at pole upper 63 and is used to place the required equipment of inoculation and carries out inoculation operation, it is preferred that two poles 63 can be respectively positioned at 61 bottom two ends of operation panel increasing stable degree.Two poles 63 are turned to into laminating chamber side wall in the second condition, operation panel 61 naturally droops laminating chamber side wall and packs up, and can both concede the space in chamber 1.
Oscillation device 7 includes carrying platform and for driving carrying platform to do the drive mechanism of horizontal reciprocating movement.Carrying platform may be disposed at the lower section of operation panel 61 and be provided with some fixed seats 71 for placing blake bottle, and the blake bottle of microbe inoculation is placed in fixed seat 711 and carries out shaken cultivation.As blake bottle has certain height, will operation table apparatus 6 be designed as can folding, you can concede space, reduce the height of chamber 1, at the same be easy to vibrate and subsequent operation carrying out.Some through holes are offered on carrying platform 71, is passed through in order to constant temperature circulating air-flow.
The integration apparatus of the present invention also include operating system, for controlling constant temperature gas bath circulating generator, air cleaner, ozone generator, uviol lamp and the oscillation device action.Control system includes main control module, power module and input module.The instruction of main control module receives input module input simultaneously accordingly controls constant temperature gas bath circulating generator, air cleaner, ozone generator, uviol lamp and oscillation device action；Power module is used to power.Main control module is micro-control unit 8, and input module is touch screen 9 in order to operate.
In one embodiment, fluorescent lamp 10 is additionally provided with chamber for illumination.Fluorescent lamp 10 equally connects and is controlled by control system.
Microorganism culture is carried out using the said equipment to comprise the following steps：
The first step, prepares culture medium in blake bottle by technological requirement, and pipette tips are filled in pipette tip box, standby Jing after high pressure steam sterilization；
Second step, opens the power supply of integration apparatus, and by touch screen arrange parameter, the parameter including but not limited to temperature of microorganism culture, duration, oscillator rotating speed, ozone generator dissipation duration, uviol lamp open duration etc..
3rd step, launches the operation panel of operation table apparatus, and the equipment such as the alcolhol burner needed for inoculation, lighter, alcohol watering can, oese, cotton ball soaked in alcohol, liquid-transfering gun and pipette tips box are positioned on operation panel.
4th step, starts constant temperature gas bath circulating generator by control system and produces constant temperature circulating gas bath, and thermostatically controlled temp is microorganism cultivation temperature ± 1 degree；Start air cleaner, the constant temperature gas bath into chamber is carried out air filtration to ensure bacteria-and dust-free；Start ozone generator, the ozone of generation carries out ozone dissipation to chamber and surrounding in the constant temperature gas bath circulatory system, now micro-control unit MCU proceeds by ozone dissipation timing, reach ozone dissipation duration and then close ozone generator, next step is carried out after 2-3 hours and is operated to ensure that ozone voluntarily decomposes completely.
5th step, keeps constant temperature gas bath circulating generator and air cleaner to be in running status, to keep the clean state in the chamber after dissipation in bacteria-and dust-free.Removable Form is pushed upwards open to the height for meeting operational requirements, the blake bottle that will be equipped with sterilising medium is placed on operation panel, starting uviol lamp carries out ultraviolet dissipation, and uviol lamp is closed after the completion of dissipation, opens fluorescent lamp lighting.
6th step, is inoculated with bacterium solution in blake bottle by microbial sterility inoculation operation requirement.After inoculation terminates, inoculation equipment is taken out, pack up the operation panel of operation table apparatus, blake bottle is placed in the fixed seat of oscillation device, start oscillation device.
7th step, according to growth of microorganism characteristic, terminates culture after reaching culture duration, oscillation device is closed successively by operating system, constant temperature gas bath circulating generator is closed, air cleaner is closed, Removable Form is opened, is turned off after taking out blake bottle, finally opening uviol lamp carries out dissipation to chamber.
Above-described embodiment is only used for a kind of microorganism culture integration apparatus for further illustrating the present invention; but the invention is not limited in embodiment; any simple modification, equivalent variations and modification that every technical spirit according to the present invention is made to above example, each fall within the protection domain of technical solution of the present invention.
Claims (10)
1. a kind of microorganism cultivates integration apparatus, it is characterised in that include：
Chamber, the chamber are provided with Removable Form；
Constant temperature gas bath circulating generator, is arranged at the chamber bottom, for producing constant temperature circulating gas in chamber
Flow to remain adapted to the temperature of microorganism culture；
Air cleaner, is arranged at the top of the chamber, for carrying out into the constant temperature circulating air-flow in chamber
Filter；
Ozone generator, is arranged at the chamber bottom, and for producing ozone, the ozone of generation is followed into constant temperature
Carrying out ozone dissipation to chamber in ring air-flow；
Uviol lamp, is arranged at the top of the chamber, for carrying out ultraviolet dissipation to chamber；
Operation table apparatus, are movably set in chamber and for carrying out inoculation operation, and the operating desk is installed on one the
Launch to form the platform being suspended from chamber under one state, the side wall for being laminated on the chamber is received under one second state；
Oscillation device, is arranged in chamber, and the oscillation device is provided with some fixed seats for placing blake bottle,
Moved with driving the blake bottle of microbe inoculation to carry out level repeatedly；
Control system, for controlling constant temperature gas bath circulating generator, air cleaner, ozone generator, the purple
Outer lamp and oscillation device action；
Microorganism culture is carried out using the equipment to comprise the following steps：Launch operation table apparatus in chamber and put
Put equipment needed for microbial inoculant；Removable Form is closed, the constant temperature gas bath circulation is started successively by control system and is sent out
Raw device produce constant temperature circulating air-flow, the air cleaner constant temperature circulating air-flow is carried out filtering, ozone generator carry out it is smelly
Oxygen dissipation；Ozone generator is closed, the blake bottle equipped with culture medium is put into after 2-3 hours and Removable Form is closed,
Starting uviol lamp carries out dissipation；Dissipation terminates to be inoculated with chamber, and operation table apparatus are packed up after inoculation, will connect
Blake bottle after kind is placed in the fixed seat of oscillation device, controls oscillation device by control system and is vibrated.
2. microorganism according to claim 1 cultivates integration apparatus, it is characterised in that：The wall body of the chamber
It is made up of corrosion resistant plate, the Removable Form is the slide type glass forms that can adjust open degree up and down.
3. microorganism according to claim 1 cultivates integration apparatus, it is characterised in that：The operation table apparatus
Including an operation panel and two adjustable shelfs, the operation panel side is rotationally connected with the wall of chamber side, and two adjustable shelfs are respectively articulated with
Between the two ends of the relative opposite side of operation panel and chamber side wall；The adjustable shelf includes two connecting rods for rotating connection,
Under the first state, two connecting rod is turned in 180 ° so that the operation panel horizontal development, in this second state
Two connecting rod turns to overlap so that the operation panel receives folded laminating chamber side wall.
4. microorganism according to claim 3 cultivates integration apparatus, it is characterised in that：The operation panel is another
The buckle being mutually matched is provided with side and correspondence chamber side wall, operation panel passes through buckle and chamber in the second state
Side wall is mutually fixed.
5. microorganism according to claim 1 cultivates integration apparatus, it is characterised in that：The operating desk dress
Put including an operation panel and two poles, the operation panel side is rotationally connected with the wall of chamber side, and two poles can horizontally rotate
Be articulated with the chamber side wall below operation panel；Two pole turns to vertical with chamber side wall in the first state
And operation panel is set up on pole, two pole turns to laminating chamber side wall, the operation panel in this second state
Sagging laminating chamber side wall.
6. microorganism according to claim 1 cultivates integration apparatus, it is characterised in that：The oscillation device
Including carrying platform and for driving carrying platform to do the drive mechanism of horizontal reciprocating movement, the fixed seat is located at carrying
On platform, the drive mechanism connects and is controlled by the control system.
7. microorganism according to claim 6 cultivates integration apparatus, it is characterised in that：The carrying platform is opened
It is provided with some through holes.
8. microorganism according to claim 1 cultivates integration apparatus, it is characterised in that：The control system bag
Include main control module, power module and input module；The instruction of main control module receives input module input is simultaneously accordingly controlled permanent
Wet bath circulating generator, air cleaner, ozone generator, uviol lamp and oscillation device action；Power module is used
In power supply.
9. microorganism according to claim 8 cultivates integration apparatus, it is characterised in that：The main control module
It is micro-control unit, the input module is touch screen.
10. microorganism according to claim 1 cultivates integration apparatus, it is characterised in that：In the chamber also
It is provided with fluorescent lamp.
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| CN201610313017.1A CN106554918B (en) | 2016-05-12 | 2016-05-12 | A kind of microculture integration apparatus |
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| CN201610313017.1A CN106554918B (en) | 2016-05-12 | 2016-05-12 | A kind of microculture integration apparatus |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110468044A (en) * | 2019-09-06 | 2019-11-19 | 重庆沐联环境工程有限公司 | A kind of microorganism constant temperature incubation integration apparatus and application method |
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| CN110468044A (en) * | 2019-09-06 | 2019-11-19 | 重庆沐联环境工程有限公司 | A kind of microorganism constant temperature incubation integration apparatus and application method |
| CN111635857A (en) * | 2020-06-19 | 2020-09-08 | 黑龙江省农业科学院土壤肥料与环境资源研究所 | Integrated equipment for avoiding contamination based on microbial cultivation |
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| CN112410213A (en) * | 2020-06-22 | 2021-02-26 | 维根技术(北京)有限公司 | Incubator and incubator sterilization method |
| CN112410213B (en) * | 2020-06-22 | 2022-10-18 | 维根技术(北京)有限公司 | Incubator and incubator sterilization method |
| DE102021110509B4 (en) | 2020-06-22 | 2023-12-14 | Wiggens Technology (Beijing) Co., Ltd. | An incubator and a sterilization process thereof |
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