CN106547099A - A kind of flow cytometer beam shaping system based on polarized light - Google Patents
A kind of flow cytometer beam shaping system based on polarized light Download PDFInfo
- Publication number
- CN106547099A CN106547099A CN201710044727.3A CN201710044727A CN106547099A CN 106547099 A CN106547099 A CN 106547099A CN 201710044727 A CN201710044727 A CN 201710044727A CN 106547099 A CN106547099 A CN 106547099A
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- Prior art keywords
- flow cytometer
- polarized light
- polarization state
- system based
- shaping system
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
- G02B27/0938—Using specific optical elements
- G02B27/095—Refractive optical elements
- G02B27/0955—Lenses
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/28—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising
- G02B27/286—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising for controlling or changing the state of polarisation, e.g. transforming one polarisation state into another
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
- G02B7/02—Mountings, adjusting means, or light-tight connections, for optical elements for lenses
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of flow cytometer beam shaping system based on polarized light, the orthopedic systems include laser instrument, polarization state generator and shaping lens, also including quarter wave plate and the components and parts of cancellation polarization.Change the polarization state of laser beam using polarization state generator, and carry out shaping to form unicellular illumination spot to which using shaping lens.The present invention proposes the polarization state for changing flow cytometer laser beam, form the polarization hot spot of unicellular illumination, polarization hot spot is introduced in cell illumination and fluorescence excitation, the beam-splitting structure and detection light spectrum of follow-up scattered light and fluorescence detection are separated and have active influence, impact of the veiling glare to the two is effectively reduced.
Description
Technical field
The present invention relates to the instrument field based on flow cytometry, and in particular to a kind of flow cytometer based on polarized light
Beam shaping system.
Background technology
Laser beam quality not only affects the overall performance of laser instrument, also greatly affects the application level of laser technology.
Generally the spatial intensity distribution of the laser beam that laser instrument sends is in Gauss distribution, i.e. Gaussian laser beam.In many applications, it is desirable to
Laser beam is to be uniformly distributed.Therefore, carrying out shaping to laser beam becomes particularly important, thereby produces various laser beams whole
Type technology, for example, LCD space light modulator, double lens reflect combined, cylindrical mirror integer etc..
The diameter of fluorescence excitation light beam and light distribution there are certain requirements in flow cytometer, existing commercial laser device
This particular/special requirement cannot be met, therefore integer need to be carried out to the outgoing beam of existing laser instrument and can adapt to liquid fluid system
Size and successful activated cell suspension in the cell to be measured that is labeled.Tested cell in laser beam illumination Cell sap stream is simultaneously
Excite fluorescence.Light distribution determines the degree that excites of tested cell, and beam sizes determine the number of cells while being excited,
The two can be had a direct impact to follow-up measurement, and the light distribution of illuminating bundle meets Gauss, during edge strength is about
The 13.5% of heart intensity, it is 78% that radius is the light intensity increase at 1/3, is that 1/10 light intensity increases as 98% at radius.At present,
The illumination spot major part of flow cytometer is all the oval-shaped beam after a kind of compromise, and size is in 60 μm of 20 μ m, its short axle
Direction is parallel with liquid stream exit direction.This light beam can provide time sense on short-axis direction, be exactly only to allow one every time
Individual cell passes through, and short axle is shorter, and the speed that cell can pass through is faster, but higher to the performance requirement of subsequent optical path detection system,
Relatively uniform illumination can be provided for the tested cell for deviateing Ye Liu centers on long axis direction.
At present, the illumination spot of flow cytometer is realized that cylindrical mirror is a kind of more by two orthogonally located cylinder microscope groups
Special Aspheric optics, its x-axis are different with the radius of y-axis, therefore lens are shaped as cylinder or semicylinder, and
Only single optical axis has image enlargement ratio, and on other direction, which functions as plate glass.Using the single of cylindrical mirror
Optical axis image amplification can carry out independent certain size integer to a certain axle of circular light spot.
The content of the invention
A kind of flow cytometer beam shaping system based on polarized light of the present invention adopts polarization optical system, using polarization
The shaping of flow cytometer illumination spot is realized in the analyzing of state generator.
The technical scheme is that：A kind of flow cytometer beam shaping system based on polarized light, the laser are whole
Shape system includes laser instrument, polarization state generator and the shaping lens being sequentially arranged along laser optical path direction.
Preferably, the position relationship of the polarization state generator and shaping lens is that polarization state generator is placed in shaping lens
Front or polarization state generator are placed in shaping lens rear.
Preferably, the light beam through the polarization state generator outgoing is full polarisation or partial polarized light.
Preferably, the shaping lens are common no polarization characteristics or with polarization characteristic.
Preferably, there is quarter wave plate in front of described polarization state generator.
Preferably, described shaping lens are made up of two non-spherical lenses, and two non-spherical lenses are mutually matched, wherein
One for aspherical concave mirror, another is aspheric surface convex mirror or is both non-spherical concave and convex mirrors.
Preferably, described laser shaping system also includes the optics for cancelling polarization state.
Preferably, described beam shaping system also includes auxiliary device, and the auxiliary device includes shell, base plate, peace
Dress laser device, installation polarization state generator and installation shaping lens device；The base plate is fixed on outer casing bottom, installs and swashs
Light device device, installation polarization state generator and installation shaping lens device are flexibly connected with base plate by securing member respectively；Install
Laser device, installation polarization state generator and installation shaping lens device are respectively equipped with through hole, the geometric center of the through hole
On same straight line.
The invention has the beneficial effects as follows：A kind of flow cytometer beam shaping system based on polarized light of the present invention is using inclined
Shake optical system, realize the polarization of flow cytometer illumination spot.The present invention proposes to change flow cytometer laser beam
Polarization state, form the polarization hot spot of unicellular illumination, polarization hot spot be introduced in cell illumination and fluorescence excitation, to follow-up
The beam-splitting structure and detection light spectrum of scattered light and fluorescence detection is separated and has active influence, can effectively reduce veiling glare to the two
Impact.
Description of the drawings
With reference to the accompanying drawing enclosed, the present invention more purpose, function and advantages are by by the as follows of embodiment of the present invention
Description is illustrated, wherein：
Fig. 1 illustrates a kind of flow cytometer beam shaping system supplymentary device design sketch based on polarized light of the present invention；
Fig. 2 illustrates CCD intensity profile discretization Gauss curve fitting figure of the present invention；
Fig. 3 schematically shows a kind of structural representation of the flow cytometer beam shaping system based on polarized light of the present invention
Figure；
Fig. 4 schematically shows a kind of 2 structure of flow cytometer beam shaping system embodiment based on polarized light of the present invention
Schematic diagram；
Fig. 5 schematically shows a kind of flow cytometer beam shaping system embodiment 3 based on polarized light of the present invention and illustrates
Figure.
Specific embodiment
By reference to one exemplary embodiment, the purpose of the present invention and function and the side for realizing these purposes and function
Method will be illustrated.However, the present invention is not limited to one exemplary embodiment disclosed below；Can by multi-form come
Which is realized.The essence of description is only to aid in the detail of the various equivalent modifications Integrated Understanding present invention.
Hereinafter, embodiments of the invention will be described with reference to the drawings.In the accompanying drawings, identical reference represents identical
Or similar part, or same or like step.
Fig. 1 is a kind of auxiliary device design sketch of the flow cytometer beam shaping system based on polarized light of the present invention.Such as
Shown in Fig. 1, light beam is sent by laser instrument, and into orthopedic systems, orthopedic systems realize two processes of shaping and polarization；Light beam is entered
System first carries out reforming process and then carries out polarization process, and can also first carry out polarization process carries out reforming process again.
Auxiliary device includes base plate 101, shell 102, installs laser device 103 as shown in Figure 1, installs polarization state and occurs
Device device 104 and installation shaping lens device 105.Laser device 103 is installed, polarization state generator device 104 and peace are installed
Dress shaping lens device 105 is fixed on above base plate 101 by removable fasteners respectively.
Base plate 101 and 102 material of shell are lightweight metal material, and base plate 101 and shell 102 are rectangle, and the base plate is put
The bottom of shell 102 is fixed on inside shell 102 and by nut.
Laser device 103 is installed and is provided with manhole, match with the outside dimension of laser instrument；Polarization state generator is installed
Device 104 is U-shaped framework structure, and the U-shaped framework is provided with rectangular through-hole, the overall dimensions of clear size of opening and polarization state generator
Matching, is easy to transmitted light beam；Shaping lens device 105 is installed for U-shaped framework structure, the U-shaped framework is provided with rectangular through-hole, just
In transmitted light beam.The geometric center of described manhole and two rectangular through-holes is on same straight line.
Fig. 2 is CCD intensity profile discretization Gauss curve fitting figure of the present invention.
The section hot spot of light beam is obtained using CCD camera, the image for collecting further is entered using Matlab softwares
Row is processed, and analyzes its size and intensity distributions.
Concrete processing procedure is to read the gray value of every pixel that CCD is collected and draw, by gray value curve discrete
Change is further carried out Gauss curve fitting and obtains CCD intensity profile discretization Gauss curve fitting figures as shown in Figure 2.Fitting shown in Fig. 2
Curve meets normal distyribution function.Matched curve is reached with initial data and is approached well, and is not affected by light intensity saturation, energy
The actual light intensity degree (not less than saturation value 255) of saturation region is estimated enough.Speckle analysis are carried out according to Gaussian parameter, according to certain
Light intensity calculates spot diameter.
Embodiment 1
In the present embodiment, laser instrument 301 is Nd:YAG fixed lasers, shaping lens are two aspheric surface convex mirror combinations
The shaping lens of formation.
Fig. 3 is a kind of structural representation of the flow cytometer beam shaping system based on polarized light of the present invention.Such as Fig. 3 institutes
Show, a kind of flow cytometer beam shaping system based on polarized light includes laser instrument 301, polarization state generator 302 and shaping
Lens 303.
Laser instrument 301 sends laser beam, and hot spot generally circular in shape changes the inclined of laser beam using polarization state generator 302
Polarization state, outgoing beam are oval hot spot, and carry out shaping to form unicellular illumination spot to which using shaping lens 303.
Embodiment 2
Fig. 4 is a kind of structural representation of the flow cytometer beam shaping system based on polarized light of the present invention.Such as Fig. 3 institutes
Show, a kind of flow cytometer beam shaping system based on polarized light includes that laser instrument 301, polarization state generator 302, shaping are saturating
Mirror 303 and cancellation polarization state optics 304.Relatively difference is the present embodiment compared with Example 1：After shaping lens 303
The optics 304 for cancelling polarization state is installed in face, is cancelled such as scrambler of optics 304 of polarization state, is disappeared using scrambler
Except polarization related damage.
Embodiment 3
In the present embodiment, laser instrument 501 is Nd:YAG fixed lasers, shaping lens 504 are aspherical concave mirror and aspheric
Face convex mirror combines the shaping lens to be formed.
Fig. 5 is a kind of structural representation of the flow cytometer beam shaping system based on polarized light of the present invention.Such as Fig. 5 institutes
Show, a kind of flow cytometer beam shaping system based on polarized light includes that laser instrument 501, quarter wave plate 502, polarization state occur
Device 503 and shaping lens 504.Relatively difference is the present embodiment compared with Example 1：Set in 503 dead ahead of polarization state generator
There is a quarter wave plate 502, the polarization direction of incident beam adjusted using quarter wave plate 502, make the polarization direction of light beam rotate θ/4,
Change the shapes and sizes of hot spot.
With reference to the explanation and practice of the present invention for disclosing here, the other embodiment of the present invention is for those skilled in the art
All will be readily apparent and understand.Illustrate and embodiment be to be considered only as it is exemplary, the present invention true scope and purport it is equal
It is defined in the claims.
Claims (8)
1. a kind of flow cytometer beam shaping system based on polarized light, the beam shaping system are included along laser optical path side
To the laser instrument, polarization state generator and shaping lens that are sequentially arranged.
2. a kind of flow cytometer beam shaping system based on polarized light according to claim 1, it is characterised in that institute
State that polarization state generator is placed in front of shaping lens or polarization state generator is placed in shaping lens rear.
3. a kind of flow cytometer beam shaping system based on polarized light according to claim 1, it is characterised in that：Jing
The light beam for crossing the polarization state generator outgoing is full polarisation or partial polarized light.
4. a kind of flow cytometer beam shaping system based on polarized light according to claim 1, it is characterised in that：Institute
It is common no polarization characteristic or with polarization characteristic to state shaping lens.
5. a kind of flow cytometer beam shaping system based on polarized light according to claim 1, it is characterised in that：Institute
State.
6. a kind of flow cytometer beam shaping system based on polarized light according to claim 1, it is characterised in that：Institute
The shaping lens stated are mutually matched by two non-spherical lenses, and for aspherical concave mirror, another is aspheric surface convex surface for one of them
Mirror is both non-spherical concave and convex mirrors.
7. a kind of flow cytometer beam shaping system based on polarized light according to claim 1, it is characterised in that：Institute
The laser shaping system stated also includes the optics for cancelling polarization state.
8. a kind of flow cytometer beam shaping system based on polarized light according to claim 1, it is characterised in that institute
The beam shaping system stated also includes auxiliary device, and the auxiliary device includes shell, base plate, installs laser device, installs
Polarization state generator and installation shaping lens device；The base plate is fixed on outer casing bottom, installs laser device, installs polarization
State generator and installation shaping lens device are flexibly connected with base plate by securing member respectively；Laser device is installed, installs inclined
Polarization state generator and installation shaping lens device are respectively equipped with through hole, and the geometric center of the through hole is on same straight line.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610951876 | 2016-11-02 | ||
| CN2016109518763 | 2016-11-02 |
Publications (1)
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| CN106547099A true CN106547099A (en) | 2017-03-29 |
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| CN201710044727.3A Pending CN106547099A (en) | 2016-11-02 | 2017-01-19 | A kind of flow cytometer beam shaping system based on polarized light |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113256630A (en) * | 2021-07-06 | 2021-08-13 | 深圳中科飞测科技股份有限公司 | Light spot monitoring method and system, dark field defect detection equipment and storage medium |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5631165A (en) * | 1994-08-01 | 1997-05-20 | Abbott Laboratories | Method for performing automated hematology and cytometry analysis |
| CN101084429A (en) * | 2004-12-17 | 2007-12-05 | 卢米尼克斯股份有限公司 | Systems, illumination subsystems, and methods for increasing fluorescence emitted by a fluorophore |
| CN101153868A (en) * | 2006-09-30 | 2008-04-02 | 深圳迈瑞生物医疗电子股份有限公司 | Stream type cell analyzer |
| CN102933964A (en) * | 2010-01-15 | 2013-02-13 | 雅培制药有限公司 | Method for determining volume and hemoglobin content of individual red blood cells |
| CN103293089A (en) * | 2013-06-13 | 2013-09-11 | 长春迪瑞医疗科技股份有限公司 | Reticulocyte analyzer |
| CN103389261A (en) * | 2013-07-23 | 2013-11-13 | 清华大学深圳研究生院 | Spatial parallel type flow cytometry measurement instrument and method |
| CN104535481A (en) * | 2014-12-29 | 2015-04-22 | 中国科学院长春光学精密机械与物理研究所 | Imaging flow cytometer |
-
2017
- 2017-01-19 CN CN201710044727.3A patent/CN106547099A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5631165A (en) * | 1994-08-01 | 1997-05-20 | Abbott Laboratories | Method for performing automated hematology and cytometry analysis |
| CN101084429A (en) * | 2004-12-17 | 2007-12-05 | 卢米尼克斯股份有限公司 | Systems, illumination subsystems, and methods for increasing fluorescence emitted by a fluorophore |
| CN101153868A (en) * | 2006-09-30 | 2008-04-02 | 深圳迈瑞生物医疗电子股份有限公司 | Stream type cell analyzer |
| CN102933964A (en) * | 2010-01-15 | 2013-02-13 | 雅培制药有限公司 | Method for determining volume and hemoglobin content of individual red blood cells |
| CN103293089A (en) * | 2013-06-13 | 2013-09-11 | 长春迪瑞医疗科技股份有限公司 | Reticulocyte analyzer |
| CN103389261A (en) * | 2013-07-23 | 2013-11-13 | 清华大学深圳研究生院 | Spatial parallel type flow cytometry measurement instrument and method |
| CN104535481A (en) * | 2014-12-29 | 2015-04-22 | 中国科学院长春光学精密机械与物理研究所 | Imaging flow cytometer |
Non-Patent Citations (1)
| Title |
|---|
| 乐家新 等: "血细胞分析技术进展与临床应用", 《继续医学教育》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113256630A (en) * | 2021-07-06 | 2021-08-13 | 深圳中科飞测科技股份有限公司 | Light spot monitoring method and system, dark field defect detection equipment and storage medium |
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Application publication date: 20170329 |