CN106544337A - A kind of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier and preparation method thereof - Google Patents
A kind of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier and preparation method thereof Download PDFInfo
- Publication number
- CN106544337A CN106544337A CN201611056500.2A CN201611056500A CN106544337A CN 106544337 A CN106544337 A CN 106544337A CN 201611056500 A CN201611056500 A CN 201611056500A CN 106544337 A CN106544337 A CN 106544337A
- Authority
- CN
- China
- Prior art keywords
- activated carbon
- herba menthae
- zymotic fluid
- carrier
- menthae powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier, its compositing formula is：Contain bacillus subtilis (Bacillus subtilis RN 61) LB culture medium zymotic fluid 375g, bacillus licheniformis (Bacillus lincheniformis RN 66) LB culture medium zymotic fluid 150g, Pseudomonas alba (Pseudomonas fluorescens RN 88) LB culture medium zymotic fluid 150g and Lactobacillus plantarum (Lactobacillus plantarum RN 53) LB culture medium zymotic fluid 75g in per 1kg finished products；Herba Menthae powder 10g, activated carbon 240g.The viable bacteria concentration of each zymotic fluid is 8,000,000,000/mL, and the diameter of activated carbon is 0.15 0.20mm, and the diameter of Herba Menthae powder is less than 0.0025mm.Invention also provides the preparation method containing Herba Menthae powder microbial bacterial agent that should be with activated carbon as carrier.The present invention solves the dust pollution question that activated carbon granule is likely to result in, high value-added product is obtained, this microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier contains a large amount of beneficial bacteriums and antipathogenic composition, absorption affinity is strong, plays the role of improved soil, pre- disease prevention, improves utilization rate of fertilizer.
Description
Technical field
The present invention relates to a kind of microbial bacterial agent, especially a kind of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier
And preparation method thereof.
Background technology
Pore structure of the activated carbon with prosperity, high adsorption capacity, intensity are big, stable chemical performance, are a kind of good
Carrier, has been applied to chemical metallurgy, oil electric power, beverage at present and has drunk water purification, the recovery of organic solvent and sewage
The aspects such as process.The loose ventilative structure of activated carbon and powerful adsorption capacity can provide attachment site and space for microorganism,
Still there are huge potentiality the aspect such as the gas permeability of soil and improved soil is improved.It is an object of the invention to provide one kind is with activity
Charcoal is microbial bacterial agent containing Herba Menthae powder of carrier and preparation method thereof, not only solves the dust dirt that activated carbon granule is likely to result in
Dye problem, and the surcharge of activated carbon can be improved, generation is conducive to soil improvement and suppresses the new of growth of pathogenic bacteria
Microbial bacterial agent.
The content of the invention
It is an object of the invention to provide a kind of microbial bacterial agent containing Herba Menthae powder and its preparation side with activated carbon as carrier
Method.
In order to solve the above problems, the present invention provide a kind of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier and its
Preparation method, its compositing formula is：Train containing bacillus subtilis (Bacillus subtilis RN-61) LB in per 1kg finished products
Foster base zymotic fluid 375g, bacillus licheniformis (Bacillus lincheniformis RN-66) LB culture medium zymotic fluid 150g,
Pseudomonas alba (Pseudomonasfluorescens RN-88) LB culture medium zymotic fluid 150g and Lactobacillus plantarum
(Lactobacillus plantarum RN-53) LB culture medium zymotic fluid 75g, Herba Menthae powder 10g, activated carbon 240g, it is described each
The viable bacteria concentration of zymotic fluid is 8,000,000,000/mL.
Invention also provides should preparation method containing Herba Menthae powder microbial bacterial agent with activated carbon as carrier, including with
Lower step：Activated carbon is made into the particle of a diameter of 0.15-0.20mm, peppermint is crushed, and crosses 500 mesh sieves, obtains diameter and be less than
The powder of 0.0025mm；By the bacterial classification of the bacillus subtilis, bacillus licheniformis, Pseudomonas alba and the Lactobacillus plantarum that preserve
It is inoculated in the LB culture mediums of sterilizing respectively, after 37 DEG C of culture 12h, ultracentrifugation, it is sending out for 8,000,000,000/mL to be adjusted to viable bacteria concentration
Zymotic fluid, takes bacillus subtilis fermentation liquor 375g, the lichen bacillus ferments liquid 150g, Pseudomonas alba zymotic fluid 150g and plant
Thing lactobacillus ferment liquid 75g, is well mixed, and by cold nebulization, is well mixed with 240g activated carbons and 10g Herba Menthae powders, makes
Containing Herba Menthae powder microbial bacterial agents of the 1kg with activated carbon as carrier.
The invention has the advantages that：The dust pollution question that activated carbon granule is likely to result in is solved, has obtained high additional
The product of value；Containing beneficial microbe and antipathogenic composition, there is improved soil, prevent the effect of soil-borne disease；After being administered in soil,
Its powerful adsorption function advantageously reduces fertilizer loss, improves utilization rate of fertilizer.
Specific embodiment
Embodiment 1
A kind of preparation method containing Herba Menthae powder microbial bacterial agent with activated carbon as carrier, comprises the following steps：
Activated carbon is made into the particle of a diameter of 0.15-0.20mm, peppermint is crushed, and crosses 500 mesh sieves, obtains diameter and be less than
The powder of 0.0025mm；By the bacterial classification of the bacillus subtilis, bacillus licheniformis, Pseudomonas alba and the Lactobacillus plantarum that preserve
It is inoculated in the LB culture mediums of sterilizing respectively, after 37 DEG C of culture 12h, ultracentrifugation, it is sending out for 8,000,000,000/mL to be adjusted to viable bacteria concentration
Zymotic fluid, takes bacillus subtilis fermentation liquor 375g, the lichen bacillus ferments liquid 150g, Pseudomonas alba zymotic fluid 150g and plant
Thing lactobacillus ferment liquid 75g, is well mixed, and by cold nebulization, is well mixed with 240g activated carbons and 10g Herba Menthae powders, makes
The pulvis of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier.
Embodiment 2
Microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier prepares suspending agent, comprises the following steps：
Activated carbon is made into the particle of a diameter of 0.15-0.20mm, peppermint is crushed, and crosses 500 mesh sieves, obtains diameter and be less than
The powder of 0.0025mm；By the bacterial classification of the bacillus subtilis, bacillus licheniformis, Pseudomonas alba and the Lactobacillus plantarum that preserve
It is inoculated in the LB culture mediums of sterilizing respectively, after 37 DEG C of culture 12h, ultracentrifugation, it is sending out for 8,000,000,000/mL to be adjusted to viable bacteria concentration
Zymotic fluid, takes bacillus subtilis fermentation liquor 375g, the lichen bacillus ferments liquid 150g, Pseudomonas alba zymotic fluid 150g and plant
Thing lactobacillus ferment liquid 75g, is well mixed, and by cold nebulization, is well mixed with 240g activated carbons and 10g Herba Menthae powders, adds
Pull open powder 30g, isopropanol 50g, wgwin D88200g, water 300g, are well mixed, make suspending agent.
Claims (4)
1. a kind of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier, its compositing formula is：Bud containing withered grass in per 1kg finished products
Spore bacillus (Bacillus subtilis RN-61) LB culture medium zymotic fluid 375g, bacillus licheniformis (Bacillus
Lincheniformis RN-66) LB culture medium zymotic fluid 150g, Pseudomonas alba (Pseudomonas fluorescens
RN-88) LB culture mediums zymotic fluid 150g and Lactobacillus plantarum (Lactobacillus plantarum RN-53) LB culture mediums are sent out
Zymotic fluid 75g, Herba Menthae powder 10g, activated carbon 240g, the viable bacteria concentration of each zymotic fluid is 8,000,000,000/mL.
2. the preparation method of new microbe agent as claimed in claim 1 with activated carbon as carrier, it is characterised in that include
Following steps：Activated carbon is made into the particle of a diameter of 0.15-0.20mm, peppermint is crushed, and crosses 500 mesh sieves, obtains diameter and be less than
The powder of 0.0025mm；By the bacterial classification of the bacillus subtilis, bacillus licheniformis, Pseudomonas alba and the Lactobacillus plantarum that preserve
It is inoculated in the LB culture mediums of sterilizing respectively, after 37 DEG C of culture 12h, ultracentrifugation, it is sending out for 8,000,000,000/mL to be adjusted to viable bacteria concentration
Zymotic fluid；Take bacillus subtilis fermentation liquor 375g, the lichen bacillus ferments liquid 150g, Pseudomonas alba zymotic fluid 150g and plant
Thing lactobacillus ferment liquid 75g, is well mixed, and by cold nebulization, is well mixed with 240g activated carbons and 10g Herba Menthae powders, makes
Containing Herba Menthae powder microbial bacterial agents of the 1kg with activated carbon as carrier.
3. the activated carbon in the microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier as claimed in claim 1, it is characterized by
Diameter is 0.15-0.20mm.
4. the Herba Menthae powder in the microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier as claimed in claim 1, it is characterized by
Diameter is less than 0.0025mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611056500.2A CN106544337A (en) | 2016-11-28 | 2016-11-28 | A kind of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611056500.2A CN106544337A (en) | 2016-11-28 | 2016-11-28 | A kind of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106544337A true CN106544337A (en) | 2017-03-29 |
Family
ID=58395140
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611056500.2A Pending CN106544337A (en) | 2016-11-28 | 2016-11-28 | A kind of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106544337A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107177528A (en) * | 2017-05-31 | 2017-09-19 | 安徽永志环能科技有限公司 | A kind of fermentation of plant straws agent and its application method |
| CN107556099A (en) * | 2017-09-06 | 2018-01-09 | 甘肃农业大学 | Special hybrid bacterial strain microbial manure of a kind of sainfoin and preparation method thereof |
| CN109331045A (en) * | 2018-11-23 | 2019-02-15 | 福建省微生物研究所 | It is a kind of containing Bee Pollen, the probiotic composition of active carbon and preparation method thereof |
| CN114015619A (en) * | 2021-12-09 | 2022-02-08 | 北京市畜牧业环境监测站 | Straw fermentation composite bacterium preparation and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101205527A (en) * | 2007-11-30 | 2008-06-25 | 上海绿乐生物科技有限公司 | Composite microbial agents and production method thereof |
| CN102115347A (en) * | 2010-12-12 | 2011-07-06 | 夏学德 | Mixture fertilizer and preparation method thereof |
| CN102212476A (en) * | 2011-03-07 | 2011-10-12 | 杭州向天歌生物科技有限公司 | Method for cleanly and efficiently producing microorganism bactericide |
| CN105254445A (en) * | 2015-10-26 | 2016-01-20 | 广西农垦国有立新农场 | Biological insecticidal organic fertilizer and preparation method thereof |
-
2016
- 2016-11-28 CN CN201611056500.2A patent/CN106544337A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101205527A (en) * | 2007-11-30 | 2008-06-25 | 上海绿乐生物科技有限公司 | Composite microbial agents and production method thereof |
| CN102115347A (en) * | 2010-12-12 | 2011-07-06 | 夏学德 | Mixture fertilizer and preparation method thereof |
| CN102212476A (en) * | 2011-03-07 | 2011-10-12 | 杭州向天歌生物科技有限公司 | Method for cleanly and efficiently producing microorganism bactericide |
| CN105254445A (en) * | 2015-10-26 | 2016-01-20 | 广西农垦国有立新农场 | Biological insecticidal organic fertilizer and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刁治民等: "《农业微生物工程学》", 31 December 2007, 青海人民出版社 * |
| 花草游戏编辑部: "《10节必学的栽培课：园艺达人养成指南》", 1 July 2016, 中原农民出版社 * |
| 范永强: "《现代中国花生栽培》", 30 November 2014, 山东科学技术出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107177528A (en) * | 2017-05-31 | 2017-09-19 | 安徽永志环能科技有限公司 | A kind of fermentation of plant straws agent and its application method |
| CN107556099A (en) * | 2017-09-06 | 2018-01-09 | 甘肃农业大学 | Special hybrid bacterial strain microbial manure of a kind of sainfoin and preparation method thereof |
| CN109331045A (en) * | 2018-11-23 | 2019-02-15 | 福建省微生物研究所 | It is a kind of containing Bee Pollen, the probiotic composition of active carbon and preparation method thereof |
| CN114015619A (en) * | 2021-12-09 | 2022-02-08 | 北京市畜牧业环境监测站 | Straw fermentation composite bacterium preparation and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106544337A (en) | A kind of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier and preparation method thereof | |
| CN110330979B (en) | Preparation method of modified biochar, composite material and soil remediation agent | |
| CN101177671B (en) | Biocontrol bacteria strain preventing and curing plant disease | |
| CN102976801A (en) | Method for producing functional microorganism organic fertilizer by using food residue | |
| CN105002221B (en) | The method of intensive Vegetable area reclaiming organic waste efficient anaerobic fermentation production biogas | |
| CN104230589A (en) | Soil improvement biofertilizer and preparation method of soil improvement biofertilizer | |
| CN102992915B (en) | Method for preparing environment-friendly and high-efficiency biological organic fertilizer by using distilled spirit vinasse | |
| WO2019061961A1 (en) | Ultra-high-temperature aerobic fermentation bacteria preparation prepared by using municipal sludge, and preparation method therefor | |
| CN105523870A (en) | Method for preparing seaweed biological bacterial fertilizer | |
| CN107512977A (en) | A kind of preparation method and applications of EM nano biologicals charcoal base soil conditioner | |
| CN104450583A (en) | Preparation method of petroleum-degrading acinetobacter junii bacillus | |
| CN110616170A (en) | Sulfonamide antibiotic polluted soil in-situ microorganism digestion preparation, preparation method and application | |
| CN106987536A (en) | Complex micro organism fungicide for river regulation and its preparation method and application | |
| CN104403973A (en) | Digestive lactobacillus alimentris with function of removing patulin and application of digestive lactobacillus | |
| CN110042072A (en) | A kind of aflatoxin degradation B1Bacterial strain and its application | |
| CN109603514A (en) | A kind of microbial deodorant and preparation method thereof | |
| CN101676242A (en) | Manufacturing method for producing anti-disease microbial fertilizer by utilizing corncob waste residue | |
| CN104152432A (en) | Petroleum hydrocarbon degrading bacteria carrier material and preparation method and purpose thereof | |
| CN1326995C (en) | Method for raising green trichoderma cellulase active by biological surfae active agent | |
| CN107410869A (en) | Cut down the method for heavy metal cadmium in rice meal using lactobacillus reuteri, lactobacillus fermenti and Lactobacillus plantarum mixed fermentation | |
| CN113321548B (en) | Organic fertilizer prepared by comprehensively utilizing wastes generated in beer production and preparation method thereof | |
| CN105586286A (en) | Method for producing heavy metal absorbent through microbiological treatment on lignite and related compound microbial agent | |
| CN102492750A (en) | Method for converting 3-cyanopyridine into nicotinic acid by using gibberella intermedia CA3-1 | |
| CN104846050A (en) | Preparation method of bacterial cellulose with antimicrobial property | |
| CN103172415B (en) | Paenibacillus polymyxa microbial fertilizer produced through resource production of surplus sludge |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170329 |
|
| RJ01 | Rejection of invention patent application after publication |