CN106525826B - Molecular smart phone rapid test method based on color dominant wavelength and complementary color wavelength - Google Patents
Molecular smart phone rapid test method based on color dominant wavelength and complementary color wavelength Download PDFInfo
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Abstract
A quick test method for a molecular smart phone based on a color dominant wavelength and a complementary color wavelength is used for preparing a sample capable of generating a color development product, collecting a color development product picture, and displaying the color dominant wavelength or the complementary color wavelength corresponding to different solutions by a smart phone detection program for the color dominant wavelength and the complementary color wavelength of the solutions. The method is used for detecting the dominant wavelength and complementary color wavelength of the color of the smart phone through colorimetric analysis, realizes the rapid quantitative detection of biochemical molecules, and has the advantages of simple and convenient operation, low cost, wide application range, suitability for wide users and the like.
Description
Technical Field
The invention relates to a quick test method of a smart phone for colorimetric analysis, in particular to a method for quantitatively detecting relevant parameters of a sample by injecting a color development product solution obtained by mixing a sample to be detected and a color developing agent into a clean and transparent reaction vessel, then placing the reaction vessel under an LED (light-emitting diode) surface light source for photographing by using the smart phone and calculating the dominant wavelength or complementary wavelength of the color of the solution to be detected by a smart phone detection program.
Background
The water quality detection method is characterized in that the water quality detection method is provided, wherein the water quality standard of the drinking water mainly considers the influence on human health, and comprises some physical indexes, chemical indexes and microorganism indexes, the pH and the nitrite concentration are two important indexes, different indexes have different standards, such as the pH range of a water sample is required to be 6.5 ~ 8.5.5, the toxicology index of the nitrite is 1mg/L, and the like.
In recent years, colorimetric methods have been widely used for rapid detection. Colorimetric methods are quantitative analytical methods, generally comprising two steps: firstly, selecting a proper color developing agent to react with a component to be detected to form a colored compound; and secondly, comparing or measuring the color depth of the colored compound so as to determine the content of the component to be measured. In the research of rapid detection and early general selection, a new colorimetric analysis method, namely a digital picture colorimetric method, is more and more widely applied, and the principle of the colorimetric analysis method comprises two parts, namely image acquisition and picture processing. Common image acquisition tools include scanners, CCD or CMOS imagers, digital cameras, smart phones, and the like; and the picture processing selects and uses different color models according to the characteristics of the color reaction, and the process is finished by professional picture processing software or writing of a mobile phone APP. Compared with a visual colorimetry and a photoelectric colorimetry, the colorimetry is simple and convenient to operate, consumes less time and is low in cost, and the detection precision is between that of the visual colorimetry and that of the photoelectric colorimetry, so that the application of the colorimetry is widely concerned.
In the digital photo colorimetry, researchers have developed several rapid detection methods in recent years. Quantitative detection of pesticide residues can be accomplished, for example, by Meng et al (X. Meng, C.W. Schultz, C. Cui, X. Li, H. Yu, On-site chip-based colorimetric estimation of organic pesticides using an organic scanner, Sensors and activators B: Chemical, 2015, 215: 577-. When Li et al (Li X, Tian J F, Shen W. Anal Bioanal Chem, 2010, 396(1): 495-501.) detect the nitrite ion concentration in a water sample, the picture is converted from an RGB mode to a gray mode in Photoshop, and then the relationship between the gray intensity and the nitrite ion concentration is analyzed. Richard et al (Murdock R C, Shen L, Optimization of a paper-based ELISA for a human performance biobased analyzer. analytical chemistry, 2013, 85(23): 11634-.
However, the above detection method has a limited application range, and is not suitable for analyzing RGB values, gray-scale values, or CMY values in a color reaction in which a color tone is significantly changed. Abe et al (K. Abe, K. Suzuki, D. Citterio, Inkjet-printed microfluidic analytical paper, analytical chemistry, 2008, 80(18): 6928-6934) prepares a microfluidic channel on a filter paper by using an Inkjet printing technology, collects a picture of color development on the filter paper by a scanner, and analyzes the color by a Lab colorimetric system, thereby realizing detection of various components in human urine. Shen et al (Shen L, Hagen J A, Papathsky I, Point-of-care colorimetric detection with a smartphone [ J]Lab on a Chip, 2012, 12(21): 4240-. Yetisen et al (Yetisen A K, Martinez-Hurtado J L, Garcia-Melenediz A, A smartphone algorithm with inter-photoresistance for the analysis of colorimetric tests [ J]Sensors and activators B: Chemical, 2014, 196: 156-. Lissian et al (Lissian, Zhang Zhengliang, Lidaichun: colorimetric quantitative determination based on dominant and complementary wavelengths of color; academic paper of analytical tests, 2016, 11) used dominant and complementary wavelengths of color in combination with MATLAB to determine pH and NO of solution2 -1And (5) carrying out quantitative detection on the concentration.
The operation method has multiple operation steps and long detection time, and the image acquisition and the image processing are carried out separately, so the operation method is not suitable for on-site rapid detection, and the work of water quality detection is difficult to be carried out in some remote areas or laggard areas. With the widespread use of smart phones, more and more researchers apply the smart phones to detection and integrate image acquisition and image processing. For example, Lopez-Ruiz et al (N, Lopez-Ruiz, V.F. Curto, M. MERENas, Smartphone-based biochemical pH and nitrate colorimetric chromatography for use in a micro fluidic device, Analytical chemistry, 2014, 86(19): 9554-9562) uses a cell phone to collect a picture of color development in a micro channel on a filter paper, and then uses cell phone software to analyze the picture to realize quantitative detection of pH value and nitrous acid. Shen et al (L, Shen, J. A. Hagen, I.Papathsky, Point-of-care colorimetric detection with a smartphone Lab on a chip, 2012, 12(21): 4240-. St prefani et Al (Andrad S I E, Lima M B, Barretoi S, A digital image-based flow-batch analyzer for determining Al (III) and Cr (VI) in water. Microchemical Journal, 2013, 109: 106-. It has the advantages of low cost and relatively short time consumption, but is relatively complex to process. Based on the digital picture colorimetry based on the smart phone and utilizing the color dominant wavelength or complementary color wavelength, the smart phone is utilized and the APP is compiled, so that the detection result is directly displayed on a mobile phone interface, and the detection time is greatly shortened.
Disclosure of Invention
The invention aims to provide a molecular smart phone rapid test method based on a color dominant wavelength and a complementary color wavelength, which realizes rapid and accurate detection of a biochemical sample based on a smart phone anytime and anywhere and has the characteristics of simple operation, low cost and wide application range.
The detection reaction vessel mentioned in the invention is a glass cuvette, a PDMS microchannel or other clean transparent vessel. The technical scheme adopted for achieving the purpose is as follows.
A quick test method for a molecular smart phone based on a dominant color wavelength and a complementary color wavelength is carried out according to the following steps:
for sample preparation capable of generating a chromogenic product;
a device for collecting a picture of a color-developed product;
the smart phone detection program is used for detecting the dominant color wavelength and complementary color wavelength of the solution color and displaying the dominant color wavelength or complementary color wavelength corresponding to different solutions;
further, the additional technical scheme is as follows:
the sample for generating the color development product is prepared from the sample which can generate the color development product and has obvious color change.
The color development product picture is obtained by taking a picture of a camera picture reaction vessel of the smart phone under the LED surface light source.
The rapid detection method comprises the steps that a smart phone App is used for photographing a reaction vessel under the condition that a sample can generate complete chromogenic reaction in a detection sample reaction vessel, the mobile phone App intercepts a reaction to obtain a picture, the picture is analyzed, and rapid quantitative detection is realized.
The method for rapidly measuring the dominant wavelength and complementary color wavelength of the color of the smart phone based on colorimetric analysis comprises the steps of mixing a sample to be measured and a corresponding color developing agent in a reaction vessel, then placing the reaction vessel under an LED (light-emitting diode) area light source, taking a picture by using a camera of the smart phone, analyzing and fitting the coded APP to obtain a calibration equation to obtain a detection result of a solution of the sample to be measured, and displaying the result on a result interface. The method realizes the detection of the relevant parameters of the solution by using the dominant wavelength and the complementary color wavelength of the color and using the detection program of the smart phone. The rapid detection method has the characteristics of low cost, strong operability, short detection time and the like. Therefore, the method has good application prospect in the fields of environment detection, water quality detection and the like, and can be particularly applied to detection of environment water quality and the like in remote areas.
Drawings
FIG. 1 is a transparent substrate layer of a detection microchannel of the invention.
FIG. 2 is a transparent channel layer of a detection microchannel of the present invention.
FIG. 3 is a transparent cover layer of the detection microchannel of the present invention.
FIG. 4 shows the addition of a chromogenic product according to the invention.
Fig. 5 is a smartphone detection program operating interface of the present invention.
In the figure: 1: a transparent substrate layer; 2: a transparent channel layer; 3: a transparent cover layer; 3-1: a chromogenic product addition well; 3-2: vent holes for the color product; 4: a liquid transferring gun; 5: detecting a program running interface by the smart phone; 5-1: a main interface; 5-2: previewing an interface; 5-3: the result is an interface.
Detailed Description
The following examples are presented to further illustrate the practice of the invention.
Example 1
A method for quickly testing a molecular smart phone based on a dominant color wavelength and a complementary color wavelength is implemented, and comprises the following steps:
(1) preparing solutions with different pH values: a0.2 mol/L sodium dihydrogen phosphate solution is prepared by using anhydrous sodium dihydrogen phosphate, a 0.1mol/L solution is prepared by using citric acid, and then the two solutions are mixed according to different proportions to obtain a solution with a pH value of 4.0 to 8.5 (pH interval is 0.5). Sodium bicarbonate solution of 0.1mol/L and sodium carbonate solution of 0.1mol/L are prepared respectively by sodium bicarbonate and sodium carbonate, and the two solutions are mixed according to different proportions to obtain solution with pH values of 9.0, 9.5 and 10.0. The pH of all solutions was determined by a STARTER 3100pH meter.
(2) preparing a pH color developing agent: 0.01g of thymol blue, 0.32g of methyl red, 1.20g of bromothymol blue and 1.20g of phenolphthalein are respectively taken, mixed and ground uniformly, 200mL of 95% absolute ethyl alcohol is taken to be dissolved fully, and 150mL of ultrapure water is added for dilution. And finally, neutralizing the solution to be just green by using a 0.1mol/L sodium hydroxide solution prepared from sodium hydroxide, and adding ultrapure water to the solution to reach the volume of 400 mL.
(3) Mixing solutions with different pH values with a color developing agent: 3mL of buffer solutions with different pH values are respectively placed in a colorimetric tube, 0.1mL of color development liquid is added into the colorimetric tube, and the colorimetric tube is shaken up to fully generate color development reaction. Subsequently, 1mL of the chromogenic product was injected into the designed microchannel.
(4) Uniform illumination of the sample: the PDMS microchannels described above were placed under an LED area light source (18 x 18cm, 20W).
(5) The smart phone detection program reading process comprises the following steps: firstly, the smart phone is fixed in front of a cuvette under an LED surface light source. Clicking an icon on a mobile phone screen to enter a main interface, wherein the main interface comprises four buttons, namely Start, History, Instructions and Quit. Clicking a Start button to enter a mobile phone camera preview interface, displaying a large red rectangular frame on the preview interface at the moment, distributing six red narrow rectangular frames in the rectangular frames, aligning each rectangular frame to a bar frame on the preview interface, clicking a Capture button, automatically intercepting a picture in the red rectangular frame by a program, storing the picture in a mobile phone, then clicking an analysis button, automatically calculating the dominant wavelength of the color in each bar frame by the program, fitting a calibration equation according to the pH value of standard liquid and the corresponding dominant wavelength of the color, calculating the pH value of a sample solution to be detected by combining the calibration equation and the dominant wavelength of the color corresponding to the solution to be detected, and finally directly displaying a detection result on the interface.
Example 2
The method of example 1 was used to detect the concentration of nitrite ions in water, and includes the following steps:
(1) preparing sodium nitrite solutions with different concentrations: 0.025 g of sodium nitrite powder was dissolved in 25 mL of ultrapure water to obtain 1000 mg/L of a sodium nitrite solution. Using the solution as mother liquor, preparing sodium nitrite solution with concentration of 0, 1, 2, 5, 10, 12, 15, 20, 25, 30 and 40 mg/L respectively.
(2) Preparing a nitrite display agent: 0.1722 g of sulfanilamide, 0.0518 g of naphthyl ethylenediamine hydrochloride and 0.317g of citric acid are dissolved in 20 mL of methanol and stored at low temperature and in dark before use.
(3) Mixing sodium nitrite and color developing agent with different concentrations: 3mL of sodium nitrite solutions with different concentrations are respectively placed in a colorimetric tube, 0.7mL of color development liquid is added into the colorimetric tube, and the colorimetric tube is shaken up to fully generate color development reaction. Subsequently, 1mL of the chromogenic product was injected into the designed microchannel.
(4) Uniform illumination of the sample: the PDMS microchannels described above were placed under an LED area light source (18 x 18cm, 20W).
(5) The smart phone detection program reading process comprises the following steps: firstly, the smart phone is fixed in front of a cuvette under an LED surface light source. Clicking an icon on a mobile phone screen to enter a main interface, wherein the main interface comprises four buttons, namely Start, History, Instructions and Quit. Clicking a Start button to enter a mobile phone camera preview interface, displaying a large red rectangular frame on the preview interface, distributing six red narrow bar-shaped frames in the rectangular frame, aligning each rectangular frame to the bar-shaped frame on the preview interface, clicking a Capture button, automatically intercepting the picture in the red rectangular frame by a program, storing the picture in the mobile phone, clicking an analysis button, automatically calculating the complementary color wavelength of the color in each bar-shaped frame by the program, and according to the NO of standard liquid2 -1Fitting the concentration and the corresponding color complementary wavelength to obtain a calibration equation, and calculating the NO of the sample solution to be detected by combining the calibration equation and the corresponding color complementary wavelength of the solution to be detected2 -1And finally, directly displaying the detection result on an interface.
Claims (1)
1. A quick test method for a molecular smart phone based on a dominant color wavelength and a complementary color wavelength is carried out according to the following steps:
for sample preparation capable of generating a chromogenic product;
the device is used for collecting the images of the color development products and is used for photographing the smart phone to collect the images;
the smart phone detection program is used for detecting the dominant color wavelength and complementary color wavelength of the solution color and displaying the dominant color wavelength or complementary color wavelength corresponding to different solutions;
the specific method for rapidly measuring is carried out according to the following specific steps:
(1) the method is used for preparing a sample capable of generating a color development product, and consists of the sample of which the color development product has obvious change along with the change of relevant parameters of the sample;
(2) the device is used for putting a sample to be detected under the device for collecting the chromogenic product to realize the chromogenic reaction of the quantitative detection of the sample, the color of the sample reaction vessel can be read out corresponding data results by the mobile phone reading device respectively, the picture information after the reaction is intercepted, the obtained picture is analyzed, and the detection result is displayed by comparing with the comparison result;
(3) according to the smart phone detection program for the dominant wavelength and complementary color wavelength of the solution color, the dominant wavelength or complementary color wavelength of the color can be automatically calculated by the smart phone detection program, a calibration equation is fitted according to the pH value of the standard solution and the corresponding dominant color wavelength, the pH value of the sample solution to be detected is calculated by combining the calibration equation and the dominant color wavelength corresponding to the solution to be detected, or the pH value of the sample solution to be detected is calculated according to the NO of the standard solution2 -1Fitting the concentration and the corresponding color complementary wavelength to obtain a calibration equation, and calculating the NO of the sample solution to be detected by combining the calibration equation and the corresponding color complementary wavelength of the solution to be detected2 -1Concentration, displaying a detection result, and writing and running the detection result on an Android mobile phone platform by using Java language;
the reaction vessel is a cuvette, PDMS microchannel or other transparent device;
the device for collecting the pictures of the color development products is used for photographing the pictures of the smart phone to collect the pictures, and is completed by placing the reaction vessel filled with the color development products under an LED surface light source;
the rapid detection method comprises the steps of taking a picture of a reaction vessel after color reaction by using a mobile phone App under the condition that a sample has complete color reaction in the sample reaction vessel, intercepting the reaction vessel according to the mobile phone App to obtain a picture, analyzing the picture, and realizing rapid quantitative detection.
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| CN107917876B (en) * | 2017-10-16 | 2020-12-22 | 太原理工大学 | Antibiotic detection device and method based on nanogold-aptamer structure |
| CN109254000A (en) * | 2018-10-25 | 2019-01-22 | 太原理工大学 | Array urine multiple determination apparatus and method based on smart machine colorimetric analysis |
| CN114264604A (en) * | 2021-12-23 | 2022-04-01 | 国家体育总局体育科学研究所 | Athlete urine color analysis system |
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