CN106520665A - Culture medium for rhodiola rosea cell - Google Patents
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- CN106520665A CN106520665A CN201611030210.0A CN201611030210A CN106520665A CN 106520665 A CN106520665 A CN 106520665A CN 201611030210 A CN201611030210 A CN 201611030210A CN 106520665 A CN106520665 A CN 106520665A
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- culture medium
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- radix rhodiolae
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
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- C12N2500/76—Undefined extracts from plants
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Abstract
The invention provides a culture medium for a rhodiola rosea cell. According to the technical scheme, a nutritional condition is improved aiming at a metabolism characteristic of the rhodiola rosea cell, plant hormones such as 6-BA, NAA, BA and IBA are added on the basis of an MS culture medium, and the culture medium is used as an inductor and a precusor substance in a synthetic medium, so that the glycoside substance secretion volume of the rhodiola rosea cell is promoted, and a culture effect of the rhodiola rosea cell is further improved through further adding a certain amount of natural medicines on the basis. An experiment discovers that after the synthetic medium provided by the invention cultures the rhodiola rosea cell, the rhodioloside secretion volume is obviously improved, and meanwhile, the cell culture density can be increased to a certain extent. The culture medium for the rhodiola rosea cell provided by the invention is improved through an innovative technology so as to realize a favorable technical effect, and is lower in cost and easy to implement at the same time so as to have a favorable popularization prospect.
Description
Technical field
The present invention relates to plant cell culture technology, designs further to the nutritional condition of plant cell, and in particular to
A kind of culture medium for Radix Rhodiolae cell.
Background technology
Plant cell culture technology is with vitro plant cell or protoplast as object, with cell concentration amplification and target generation
Thank to the culture process for the purpose of product accumulation.For the cell culture for the purpose of obtaining specific metabolite, which is main
Process procedure is not only in that the amplification of cell concentration, meanwhile, the induction synthesis of target metabolic product is even more important to cultivating benefit.
In the incubation of plant cell, condition of culture and nutritional condition are the most important factors for affecting yield, and wherein condition of culture is
Refer to the control of the environmental conditions such as temperature, dissolved oxygen, illumination, pH, and nutritional condition refers mainly to the culture medium of plant cell.Due in envelope
Close the sole nutrition source that culture medium in culture environment is plant cell, therefore growth water of the composition of culture medium to plant cell
Flat, metabolite classification has extremely important impact, particularly with the cell culture for the purpose of obtaining specific metabolite
For, culture medium often affects the key factor of culture efficiency.
Rhodiola Crassulaceae herbaceos perennial, is one of important Tibetan medicine medicinal plants. mainly originate in China Tibet,
The high altitude localitiess such as Yunnan, Sichuan.In vivo containing multiple compounds such as distinctive rhodioloside, butyl alcohols, rhodioloside has anti-tired
The remarkable efficacies such as labor, defying age, resisting microwave radiation, antiviral and antitumor.Studied in recent years and also found, rhodioloside was in military affairs
Also there is in medical science, aerospace medicine and sports medical science highly important value.But due to China wild Radix Rhodiolae resource very
Limited, Radix Rhodiolae again more is grown in height above sea level area higher, is often difficult to obtain more propagating materialss, in addition long-term predatoriness
Excavate and rhizome putrefactive phenomenon is extremely serious when introducing a fine variety so that Radix Rhodiolae has become Endangered species.Therefore, Radix Rhodiolae is on the spot
Preserve and ex-situ cultivation all becomes more difficult.Techniques of in Vitro Culture collects amplification in plant germplasm and middle or short term preserves and serves
Great role, but be limited by culture technique and relatively lag behind, especially the design of Radix Rhodiolae cytotrophy delivery system is not reasonable, makes
Radix Rhodiolae isolated cells that more slow, rhodioloside yield is grown under condition of suspension culture is relatively low.
The content of the invention
It is contemplated that for the technological deficiency of prior art, there is provided for the culture medium of Radix Rhodiolae cell, existing to solve
There is the technical problem of Radix Rhodiolae cell culture effect on driving birds is not good in technology.
The invention solves the problems that another technical problem be prior art Radix Rhodiolae cell cultivation process in rhodioloside produce
Amount is relatively low.
To realize above technical purpose, the present invention is employed the following technical solutions：
A kind of culture medium for Radix Rhodiolae cell, the culture medium contain the composition of MS culture medium, while also containing plant
Hormone 6-BA 1mg/L, phytohormone NAA 2mg/L, phytohormone BA 3mg/L, phytohormone IBA 1mg/L, tyrosinase 15～
10mg/L, 0.5～1mmol/L of vitamin C, 10～50mg/L of Phenylalanine, 10～50mg/L of cinnamic acid, salicylic acid 0.1～
1mg/L, 200～500mg/L of peptone, salicylic acid 0.1mg/L.
Preferably, Caulis Bauhihiae Championii squeezing juice of the culture medium also containing 1g/L.
Preferably, Herba Mimosae Pudicae squeezing juice of the culture medium also containing 1g/L.
Preferably, weldering dish squeezing juice of the culture medium also containing 1g/L.
Preferably, river Pennisetum centrasiaticum Tzvel squeezing juice of the culture medium also containing 1g/L.
Preferably, the culture medium contains the composition of MS culture medium, while also containing phytohormone 6-BA 1mg/L, plant
Hormone NAA 2mg/L, phytohormone BA 3mg/L, phytohormone IBA 1mg/L, L-Tyrosine 7mg/L, vitamin C 0.8mmol/
L, phenylalanine-3,4-quinone 0mg/L, cinnamic acid 30mg/L, salicylic acid 0.6mg/L, peptone 350mg/L, salicylic acid 0.1mg/L, cross hilllock
Imperial squeezing juice 1g/L, Herba Mimosae Pudicae squeezing juice 1g/L, weld dish squeezing juice 1g/L, river Pennisetum centrasiaticum Tzvel squeezing juice 1g/L.
In above technical scheme, the MS culture medium can be prepared according to the general technology general knowledge of this area, when
The MS culture medium of formula in detail below can also so be adopted：NH4NO31650mg/L, KNO31900mg/L, CaCl2·2H2O
440mg/L, MgSO4·7H2O 370mg/L, KH2PO4170mg/L, KI 0.83mg/L, H3BO36.2mg/L, MnSO4·4H2O
22.3mg/L, ZnSO4·7H2O 8.6mg/L, Na2MoO4·2H2O 0.25mg/L, CuSO4·5H2O 0.025mg/L,
CoCl2·6H2O 0.025mg/L, FeSO4·7H2O 27.8mg/L, Na2-EDTA·2H2O 37.3mg/L, inositol 100mg/L,
Nicotinic acid 0.5mg/L, vitamin B6 0.5mg/L, vitamin B1 0.1mg/L, glycine 2mg/L, water surplus.
In above technical scheme, Caulis Bauhihiae Championii, Herba Mimosae Pudicae, weldering dish, river Pennisetum centrasiaticum Tzvel refer to its fresh herb respectively.Institute in the present invention
The squeezing juice stated, refers to the Fresh sap of each raw material obtained using physical method, including but not limited to squeezing, crushing centrifugation etc.
Conventional method.
The invention provides a kind of culture medium for Radix Rhodiolae cell, the technical scheme is for Radix Rhodiolae cellular metabolism spy
Property improves its nutritional condition, with the addition of the phytohormone such as 6-BA, NAA, BA, IBA, using which as conjunction on MS medium bases
Elicitor and precursor substance in culture medium, promote the glycoside material secretory volume of Radix Rhodiolae cell, on this basis further
A certain amount of natural drug be with the addition of so that the culture effect of Radix Rhodiolae cell is had been further upgraded.Experiment discovery, works as profit
After being cultivated to Radix Rhodiolae cell with synthetic medium of the present invention, rhodioloside secretory volume is obviously improved, while can be certain
Degree promotes cell culture density.The present invention good technique effect is realized with the technological improvement of novelty, while cost compared with
It is low, be easily achieved, therefore move back promotion prospect with good.
Specific embodiment
Hereinafter the specific embodiment to the present invention is described in detail.In order to avoid excessive unnecessary details,
Will not be described in detail to belonging to known structure or function in following examples.In addition to being defined, institute in following examples
Technology and scientific terminology are with the identical meanings being commonly understood by with those skilled in the art of the invention.
Embodiment 1
A kind of culture medium for Radix Rhodiolae cell, the culture medium contain the composition of MS culture medium, while also containing plant
Hormone 6-BA 1mg/L, phytohormone NAA 2mg/L, phytohormone BA 3mg/L, phytohormone IBA 1mg/L, L-Tyrosine
5mg/L, vitamin C 0.5mmol/L, Phenylalanine 10mg/L, cinnamic acid 10mg/L, salicylic acid 0.1mg/L, peptone
200mg/L, salicylic acid 0.1mg/L.
Embodiment 2
A kind of culture medium for Radix Rhodiolae cell, the culture medium contain the composition of MS culture medium, while also containing plant
Hormone 6-BA 1mg/L, phytohormone NAA 2mg/L, phytohormone BA 3mg/L, phytohormone IBA 1mg/L, L-Tyrosine
10mg/L, vitamin C 1mmol/L, Phenylalanine 50mg/L, cinnamic acid 50mg/L, salicylic acid 1mg/L, peptone 500mg/L,
Salicylic acid 0.1mg/L.
Embodiment 3
A kind of culture medium for Radix Rhodiolae cell, the culture medium contain the composition of MS culture medium, while also containing plant
Hormone 6-BA 1mg/L, phytohormone NAA 2mg/L, phytohormone BA 3mg/L, phytohormone IBA 1mg/L, L-Tyrosine
7mg/L, vitamin C 0.8mmol/L, phenylalanine-3,4-quinone 0mg/L, cinnamic acid 30mg/L, salicylic acid 0.6mg/L, peptone
350mg/L, salicylic acid 0.1mg/L, Caulis Bauhihiae Championii squeezing juice 1g/L.
Embodiment 4
A kind of culture medium for Radix Rhodiolae cell, the culture medium contain the composition of MS culture medium, while also containing plant
Hormone 6-BA 1mg/L, phytohormone NAA 2mg/L, phytohormone BA 3mg/L, phytohormone IBA 1mg/L, L-Tyrosine
7mg/L, vitamin C 0.8mmol/L, phenylalanine-3,4-quinone 0mg/L, cinnamic acid 30mg/L, salicylic acid 0.6mg/L, peptone
350mg/L, salicylic acid 0.1mg/L, Herba Mimosae Pudicae squeezing juice 1g/L.
Embodiment 5
A kind of culture medium for Radix Rhodiolae cell, the culture medium contain the composition of MS culture medium, while also containing plant
Hormone 6-BA 1mg/L, phytohormone NAA 2mg/L, phytohormone BA 3mg/L, phytohormone IBA 1mg/L, L-Tyrosine
7mg/L, vitamin C 0.8mmol/L, phenylalanine-3,4-quinone 0mg/L, cinnamic acid 30mg/L, salicylic acid 0.6mg/L, peptone
350mg/L, salicylic acid 0.1mg/L, weld dish squeezing juice 1g/L.
Embodiment 6
A kind of culture medium for Radix Rhodiolae cell, the culture medium contain the composition of MS culture medium, while also containing plant
Hormone 6-BA 1mg/L, phytohormone NAA 2mg/L, phytohormone BA 3mg/L, phytohormone IBA 1mg/L, L-Tyrosine
7mg/L, vitamin C 0.8mmol/L, phenylalanine-3,4-quinone 0mg/L, cinnamic acid 30mg/L, salicylic acid 0.6mg/L, peptone
350mg/L, salicylic acid 0.1mg/L, river Pennisetum centrasiaticum Tzvel squeezing juice 1g/L.
Embodiment 7
A kind of culture medium for Radix Rhodiolae cell, the culture medium contain the composition of MS culture medium, while also containing plant
Hormone 6-BA 1mg/L, phytohormone NAA 2mg/L, phytohormone BA 3mg/L, phytohormone IBA 1mg/L, L-Tyrosine
7mg/L, vitamin C 0.8mmol/L, phenylalanine-3,4-quinone 0mg/L, cinnamic acid 30mg/L, salicylic acid 0.6mg/L, peptone
350mg/L, salicylic acid 0.1mg/L, Caulis Bauhihiae Championii squeezing juice 1g/L, Herba Mimosae Pudicae squeezing juice 1g/L weld dish squeezing juice 1g/L, river Pennisetum centrasiaticum Tzvel
Squeezing juice 1g/L.
Embodiment 8
The present embodiment be used to evaluating culture medium that above example 1～7 provided as during synthetic medium to Radix Rhodiolae
The impact of rhodioloside yield in cell, experimental result are as shown in table 1.
1 different culture media of table is used as impact during synthetic medium to rhodioloside yield in Radix Rhodiolae cell
When as can be seen here, by the use of culture medium provided by the present invention as synthetic medium culture Radix Rhodiolae cell, to which
Rhodioloside yield has definite facilitation.
Above embodiments of the invention are described in detail, but the content have been only presently preferred embodiments of the present invention,
Not to limit the present invention.All any modification, equivalent and improvement made in the application range of the present invention etc., all should
It is included within protection scope of the present invention.
Claims (6)
1. a kind of culture medium for Radix Rhodiolae cell, it is characterised in that the culture medium contains the composition of MS culture medium, while also
Containing phytohormone 6-BA 1mg/L, phytohormone NAA 2mg/L, phytohormone BA 3mg/L, phytohormone IBA 1mg/L,
Tyrosinase 15～10mg/L, 0.5～1mmol/L of vitamin C, 10～50mg/L of Phenylalanine, 10～50mg/L of cinnamic acid, bigcatkin willow
Acid 0.1～1mg/L, 200～500mg/L of peptone, salicylic acid 0.1mg/L.
2. a kind of culture medium for Radix Rhodiolae cell according to claim 1, it is characterised in that the culture medium also contains
The Caulis Bauhihiae Championii squeezing juice of 1g/L.
3. a kind of culture medium for Radix Rhodiolae cell according to claim 1, it is characterised in that the culture medium also contains
The Herba Mimosae Pudicae squeezing juice of 1g/L.
4. a kind of culture medium for Radix Rhodiolae cell according to claim 1, it is characterised in that the culture medium also contains
The weldering dish squeezing juice of 1g/L.
5. a kind of culture medium for Radix Rhodiolae cell according to claim 1, it is characterised in that the culture medium also contains
The river Pennisetum centrasiaticum Tzvel squeezing juice of 1g/L.
6. a kind of culture medium for Radix Rhodiolae cell according to claim 1, it is characterised in that the culture medium contains MS
The composition of culture medium, while also contain phytohormone 6-BA 1mg/L, phytohormone NAA 2mg/L, phytohormone BA 3mg/L,
Phytohormone IBA 1mg/L, L-Tyrosine 7mg/L, vitamin C 0.8mmol/L, phenylalanine-3,4-quinone 0mg/L, cinnamic acid 30mg/L,
Salicylic acid 0.6mg/L, peptone 350mg/L, salicylic acid 0.1mg/L, Caulis Bauhihiae Championii squeezing juice 1g/L, Herba Mimosae Pudicae squeezing juice 1g/L,
Weldering dish squeezing juice 1g/L, river Pennisetum centrasiaticum Tzvel squeezing juice 1g/L.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611030210.0A CN106520665A (en) | 2016-11-15 | 2016-11-15 | Culture medium for rhodiola rosea cell |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611030210.0A CN106520665A (en) | 2016-11-15 | 2016-11-15 | Culture medium for rhodiola rosea cell |
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| CN201611030210.0A Pending CN106520665A (en) | 2016-11-15 | 2016-11-15 | Culture medium for rhodiola rosea cell |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022117230A1 (en) | 2020-12-02 | 2022-06-09 | De Klebnikoff, Nicolas | Cosmetic composition based on cells of rhodiola rosea |
| BE1028846A1 (en) | 2020-12-02 | 2022-06-27 | De Klebnikoff Nicolas | Cosmetic composition based on Rhodiola Rosea cells |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1417343A (en) * | 2001-10-31 | 2003-05-14 | 东北林业大学 | Plant callus particls suspending culture to produce rhodiola glycoside |
| CN101531991A (en) * | 2009-04-17 | 2009-09-16 | 北京林业大学 | Rhodida plant callus and suspension cell granule culture method |
| CN101836585A (en) * | 2009-03-19 | 2010-09-22 | 中国科学院过程工程研究所 | Tissue-culture seedling raising method of rhodiola crenulata |
| CN105191790A (en) * | 2015-08-20 | 2015-12-30 | 中央民族大学 | In-vitro culturing method for rhodiola dumulosa |
-
2016
- 2016-11-15 CN CN201611030210.0A patent/CN106520665A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1417343A (en) * | 2001-10-31 | 2003-05-14 | 东北林业大学 | Plant callus particls suspending culture to produce rhodiola glycoside |
| CN101836585A (en) * | 2009-03-19 | 2010-09-22 | 中国科学院过程工程研究所 | Tissue-culture seedling raising method of rhodiola crenulata |
| CN101531991A (en) * | 2009-04-17 | 2009-09-16 | 北京林业大学 | Rhodida plant callus and suspension cell granule culture method |
| CN105191790A (en) * | 2015-08-20 | 2015-12-30 | 中央民族大学 | In-vitro culturing method for rhodiola dumulosa |
Non-Patent Citations (1)
| Title |
|---|
| 吴双秀: "高山红景天颗粒状愈伤组织悬浮培养和红景天试的诱导", 《中国博士学位论文全文数据库 农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022117230A1 (en) | 2020-12-02 | 2022-06-09 | De Klebnikoff, Nicolas | Cosmetic composition based on cells of rhodiola rosea |
| BE1028846A1 (en) | 2020-12-02 | 2022-06-27 | De Klebnikoff Nicolas | Cosmetic composition based on Rhodiola Rosea cells |
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