CN106517507B - Method and device for quickly starting anaerobic ammonia oxidation at normal temperature - Google Patents
Method and device for quickly starting anaerobic ammonia oxidation at normal temperature Download PDFInfo
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Abstract
The invention relates to a method and a device for quickly starting anaerobic ammonia oxidation at normal temperature, wherein the method comprises the following steps: 1) Inoculating common activated sludge in the reactor; 2) Starting an oxygen-limited autotrophic nitrification-denitrification process under the condition of normal temperature and low substrate: preparing simulated wastewater according to an OLAND culture medium, continuously flowing into a reactor through a water inlet pump, and feeding initial NH 4 + The concentration range of N is 20-30mg/L, the dissolved oxygen is controlled at 0.5-1mg/L in the stage, the hydraulic retention time is 2-4 days, and NH in the inlet water is gradually increased 4 + N concentration, NH when influent 4 + Increasing the concentration of N to 100mg/L, and enabling the removal rate of ammonia nitrogen and total nitrogen in effluent to be more than 90%, so as to realize the start of an OLAND process at normal temperature and obtain an OLAND biomembrane; 3) Starting an Anammox process under the condition of normal temperature: preparing simulated wastewater according to an Anammox culture medium, adopting a continuous water inlet mode for reactor operation, and adopting NH 4 + -N and NO 2 ‑ Initial concentration of-N is 60-80 mg/L, hydraulic retention time is 2-4 days, and NH in inlet water is gradually increased 4 + -N and NO 2 ‑ N concentration as effluent NH 4 + -N and NO 2 ‑ The removal rate of-N is more than 95%, and the anaerobic ammonia oxidation process is successfully started at normal temperature.
Description
Technical Field
The invention relates to the technical field of novel sewage denitrification, in particular to a method and a device for quickly starting anaerobic ammonia oxidation at normal temperature.
Background
With the acceleration of industrialization and urbanization, a large amount of nitrogen-containing wastewater is discharged into the natural environment, and the nitrogen pollution situation of the water body is increasingly severe. Nitrogen circulation in the water environment is destroyed, and nitrogen pollution of the water body is induced. Ammonia nitrogen is one of main pollutants in wastewater discharged by China, and nitrogen pollution control, particularly high ammonia nitrogen wastewater treatment, is a urgent priority for water pollution treatment in China. The conventional biological denitrification technology is based on nitrification-denitrification reaction, wherein ammonia nitrogen is converted into nitrite nitrogen or nitrate nitrogen in a nitrification stage, and the nitrite nitrogen or the nitrate nitrogen is converted into nitrogen again in a denitrification stage, so that the wastewater denitrification is realized. With the increasing trend of nitrogen pollution and the improvement of sewage discharge standard, the traditional denitrification process has many defects, for example, when high ammonia nitrogen wastewater is treated, a large amount of oxygen is required to be supplied in a nitrification stage, a large amount of organic electron donors are additionally added in a denitrification stage, acid and alkali regulation is required in two stages, the material consumption and energy consumption are greatly increased, and the treatment effect is not ideal enough.
Anammox (Anammox) is a novel biological denitrification technology suitable for high ammonia nitrogen wastewater treatment, and is one of the important support technologies for controlling the nitrogen pollution of the water body at present. Anammox is the biochemical process of Anammox bacteria oxidizing ammonia nitrogen into nitrogen gas with nitrite nitrogen as electron acceptor under anaerobic condition. The Anammox process is an anaerobic autotrophic biochemical process, does not need oxygen supply and an additional organic electron donor, has extremely high denitrification efficiency and does not generate carbon dioxide in reaction. Therefore, anammox is an efficient, economical and environment-friendly denitrification technology, and attracts extensive attention in the research field. However, the Anammox bacteria grow slowly, have a long generation time (about 11 days), and are susceptible to external environmental conditions (temperature, pH, dissolved oxygen, etc.), and thus the starting of the Anammox device is difficult. The choice of reactor is particularly important for the rapid start-up of the Anammox process. The start of the Anammox process is essentially a process of Anammox bacteria enrichment and its activity enhancement. The selected reactor should have high efficiency biological retention rate and be suitable for long-term enrichment culture of slow-growing Anammox bacteria. A fixed bed reactor is one of the devices suitable for start-up of the Anammox process. Strous et al (Strous M., van Gerven E., zheng P., et al, 1997.Ammonium removed from centralized water streams with the anaerobic ammonium oxidation (anamox) process in differential reaction, water Research, 31-1952.) successfully started the Anamox process for the first time with an upflow fixed bed reactor, however, the filler of this test was sintered glass beads, resulting in problems with difficult discharge of large amounts of bubbles generated during the reaction and clogging of the packing layer, which was not stable enough after 60 days of operation. Fujii et al (Fujii T., sugino H., rouse J.D., et al.,2002.Characterization of the microbial communication in an anaerobic ammonium-oxidizing bio-membrane concentrated on a non-woven bio-reactor carrier. Journal of Bioscience and Bioengineering,94 (5): 412-418.) use of a non-woven fabric filler improves the efficiency of the up-flow fixed bed reactor in retaining the Anammox bacteria and also improves the stability of the Anammox process operation, which is disadvantageous in that the non-woven fabric is used as the filler, and excessive thickness of the Anammox biofilm on the non-woven fabric affects the mass transfer of the substrate to cause the biofilm to blacken or autolysis of the cells.
At present, the Anammox starting period is still long (generally more than 3 months), most Anammox starting devices are controlled to be 35-37 ℃, constant-temperature hot water in the temperature range is obtained through a heating rod and a temperature controller, and temperature control is realized through constant-temperature hot water bath, so that the long-term operation of the temperature control system can bring about consumption of a large amount of electric energy, and the operation cost is increased; in addition, the temperature controller will break down, leading to the heating rod out of control, can influence the device performance, bring the potential safety hazard even. Therefore, further optimization of the choice, structure and operating strategy of the packing is required to improve the performance of the fixed bed start-up Anammox process. Meanwhile, in order to save energy consumption caused by temperature control, improve the safety and stability of device operation, and widen the applicable conditions of the Anammox process, it is urgently needed to establish a high-efficiency Anammox starting device operating at normal temperature.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to solve the technical problem of providing a method and a device for quickly starting anaerobic ammonia oxidation at normal temperature. The starting method is used for inoculating common activated sludge at normal temperature, firstly an oxygen-limited autotrophic nitrification-denitrification (OLAND) process is started to culture an OLAND biomembrane, and then the Anammox process is started by enriching Anammox bacteria by the biomembrane. The method can greatly shorten the starting time of the Anammox process, improve the treatment capacity of the nitrogen-containing sewage and save energy consumption. The starting device is constructed according to the starting method, so that the starting process can be accelerated, the starting effectiveness can be improved, and meanwhile, the stable and efficient denitrification performance can be realized under the normal temperature condition without temperature control. This starting drive need not temperature controller and heating rod, through setting up biological nest type filler, foraminiferous baffle, and lay the non-woven fabrics on foraminiferous baffle, the function bacterial species volume that has showing and having improved the main reaction zone, improve the mass transfer between function fungus and the substrate, the operation effect of device has been guaranteed, device simple structure, operation control is more convenient, and long-term operation's security is high, the operation process is more reliable, therefore, but investment and the running cost of greatly reduced Anammox technology in engineering application, satisfy the actual production needs.
The technical scheme adopted by the invention for solving the technical problems is that,
a method for quickly starting up anaerobic ammonia oxidation at normal temperature comprises the following steps:
1) Inoculating common activated sludge into the reactor, wherein the common activated sludge is aerobic activated sludge, nitrified sludge or denitrifying sludge, the suspended solid concentration MLSS of the inoculated sludge mixed solution is 2000-3000mg/L, and MLVSS/MLSS =0.6-0.85;
2) Starting an oxygen-limited autotrophic nitrification-denitrification (OLAND) process under the condition of normal temperature and low substrate: preparing simulated wastewater according to an OLAND culture medium, continuously flowing into a reactor through a water inlet pump, and feeding initial NH 4 + The concentration range of N is 20-30mg/L, the Dissolved Oxygen (DO) is controlled at 0.5-1mg/L in the stage, the hydraulic retention time is 2-4 days, and NH in the inlet water is gradually increased 4 + N concentration, NH when influent 4 + The concentration of N is increased to 100mg/L, the removal rate of the ammonia nitrogen and the total nitrogen in the effluent is over 90 percent, the start of an OLAND process at normal temperature is realized, and O is obtainedLAND biomembrane to realize pre-enrichment of Anammox (Anamox) bacteria;
3) Starting an Anammox process under the condition of normal temperature: preparing simulated wastewater according to an Anammox culture medium, and adopting a continuous water inlet mode for reactor operation, namely NH 4 + -N and NO 2 - Initial concentration of-N is 60-80 mg/L, hydraulic retention time is 2-4 days, and NH in inlet water is gradually increased 4 + -N and NO 2 - N concentration, stepwise enrichment of Anammox bacteria, NH when effluent 4 + -N and NO 2 - The removal rate of-N is more than 95%, the transition from OLAND biomembrane to Anammox biomembrane is realized, and the anaerobic ammonia oxidation process is successfully started at normal temperature.
A normal-temperature rapid starting device for anammox is characterized in that the device is constructed based on a fixed bed reactor by applying the starting method, and comprises a device body, a water inlet tank, a water seal structure and a water collecting tank,
the device body is a cylinder and comprises an inner layer pipe and an outer layer pipe, an air interlayer is formed between the inner layer pipe and the outer layer pipe, the cylinder space of the inner layer pipe is a main reaction area, perforated clapboards are arranged at the middle part and the bottom part of the main reaction area of the inner layer pipe, non-woven fabrics are laid on each perforated clapboard, the main reaction area of the inner layer pipe is divided into an upper layer and a lower layer by the perforated clapboards at the middle part and the bottom part, namely, an area between the perforated clapboard at the middle part and the perforated clapboard at the bottom part is a lower packing layer, and an area above the perforated clapboard at the middle part is an upper packing layer; biological nest type fillers are filled in the lower layer filler layer and the upper layer filler layer; the lower part of the main reaction zone is a funnel-shaped sludge hopper, the bottom end of the sludge hopper is provided with a water inlet and a sludge circulation bottom outlet, the upper part of the main reaction zone is provided with a sludge circulation top inlet and a water outlet, the water inlet is connected with a water inlet tank through a water inlet pump and a water inlet pipe, the sludge circulation bottom outlet is connected with the sludge circulation top inlet through an external sludge pipeline, the water outlet is connected with one end of a water outlet pipe, and the other end of the water outlet pipe is immersed in the water collecting tank, so that the device body is isolated from the air; and a gas discharge port is arranged at the top of the main reaction zone and is connected with the water seal structure through an exhaust pipe.
Compared with the prior art, the invention has the following beneficial effects:
1) Compared with most of Anammox process starting schemes (the temperature is controlled to be 35-37 ℃), the device does not need temperature control, greatly reduces energy consumption, realizes energy conservation and remarkable reduction of operation cost, and also improves the safety of long-term operation of the device; and the Anammox starting period at the temperature is about 40 days, and compared with most of the current Anammox starting periods (more than 3 months), the starting process is greatly shortened.
2) In the starting method, an OLAND process is started under the condition of normal temperature and low substrate (the initial ammonia nitrogen concentration in inlet water is 20-30 mg/L), an OLAND biomembrane is cultured, the process of starting the Anammox process from the OLAND biomembrane directly enters an Anammox activity expression period, and compared with the process of starting the Anammox process from common activated sludge (common activated sludge is inoculated or the Anammox process is directly started from the Anammox sludge), the starting method has no cell autolysis period and stagnation period, and shortens the starting process of the Anammox process; meanwhile, preaccumulated Anammox bacteria in OLAND biofilms increase the effectiveness and stability of subsequent Anammox process start-up.
3) According to the starting device, the packing layer of the starting device adopts biological nest type packing, the whole packing layer is layered into an upper part and a lower part through the two porous clapboards, and the non-woven fabric is laid on each porous clapboard, so that the interception effect of the reactor on functional bacteria can be enhanced, and the nitrogen generated by reaction can be easily discharged; the middle part of the clapboard with holes divides the packing layer into an upper packing layer and a lower packing layer, so that functional bacteria are better distributed in the upper packing layer and the lower packing layer, the reaction contact time between a substrate and a biological phase is prolonged, the mass transfer between the substrate and the biological phase is strengthened, and the strengthening anaerobic ammonia oxidation starting efficiency of the fixed bed reactor is improved.
4) The lower part of the device body of the starting device is provided with the sludge bucket and the external sludge circulating system, the sludge bucket collects the sludge settled on the upper part, the redistribution of the sludge in the main reaction zone and the re-filming on the filler are realized through the external sludge circulating system, the substrate mass transfer and the interception of the reactor on functional bacteria are enhanced, and the anaerobic ammonia oxidation starting efficiency is further enhanced.
5) The air interlayer formed between the inner layer pipe and the outer layer pipe in the device body of the starting device can effectively buffer the influence of the indoor temperature change outside the reactor (device body) on the activity of functional bacteria.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of an anammox rapid start-up device at room temperature according to the present invention.
FIG. 2 is a schematic view of the structure of biological nest type filler of one embodiment of the invention.
In the figure: 1, a water inlet tank; 2, water inlet pipe; 3, a water inlet pump; 4, a water inlet; 5, a base; 6, a sludge circulation bottom outlet; 7 a clapboard with holes; 8, an air interlayer; 9, a sludge hopper; 10 biological nested fillers; 11 a sludge circulation top inlet; 12 water outlet; 13 water outlet pipe; 14 a water collection tank; 15 an exhaust port; 16 an exhaust pipe; 17, a water seal structure; 101 a tiny dentate structure; 102 a gear-like structure.
Detailed Description
The present invention is described in detail below with reference to the examples and the accompanying drawings, but the present invention is not limited thereto.
The method for quickly starting the anaerobic ammonia oxidation at normal temperature (starting method or method for short) comprises the following steps:
1) Inoculating common activated sludge (aerobic activated sludge, nitrified sludge or denitrifying sludge) in a reactor, wherein the suspended solid concentration MLSS of the inoculated sludge mixed solution is 2000-3000mg/L, and MLVSS/MLSS =0.6-0.85;
2) Starting an oxygen-limited autotrophic nitrification-denitrification (OLAND) process under the condition of normal temperature: preparing simulated wastewater according to an OLAND culture medium, continuously flowing into a reactor through a water inlet pump, and feeding initial NH 4 + The concentration range of N is 20-30mg/L, the Dissolved Oxygen (DO) is controlled at 0.5-1mg/L in the stage, the hydraulic retention time is 2-4 days, and NH in the inlet water is gradually increased 4 + N concentration, NH when influent 4 + The concentration of N is increased to 100mg/L, the removal rate of ammonia nitrogen and total nitrogen in effluent is over 90 percent, the start of an OLAND process at normal temperature is realized, and an OLAND biomembrane (with the main function of the OLAND biomembrane) is obtainedThe bacteria are Ammonia Oxidizing Bacteria (AOB) and Anammox bacteria, so that the pre-enrichment of the Anammox bacteria is realized;
3) Starting an Anammox process under the condition of normal temperature: preparing simulated wastewater according to an Anammox culture medium, adopting a continuous water inlet mode for reactor operation, and adopting NH 4 + -N and NO 2 - Initial concentration of-N is 60-80 mg/L, hydraulic retention time is 2-4 days, and NH in inlet water is gradually increased 4 + -N and NO 2 - N concentration, stepwise enrichment of Anammox bacteria, NH when effluent 4 + -N and NO 2 - The removal rate of-N is more than 95%, the transition from OLAND biomembrane to Anammox biomembrane is realized, and the anaerobic ammonia oxidation process is successfully started at normal temperature.
The reactor in the above process is referred to as the apparatus body in the starting apparatus of the present invention.
The invention discloses a normal-temperature anaerobic ammonia oxidation quick starting device (a starting device or a device for short, see figure 1) which is constructed based on a fixed bed reactor and comprises a device body, a water inlet tank 1, a water seal structure 17 and a water collecting tank 14,
the device body is a cylinder, the bottom of the device body is connected with a base 5, a top cover is arranged at the top of the device body, the device body comprises an inner layer pipe and an outer layer pipe, an air interlayer 8 is formed between the inner layer pipe and the outer layer pipe, the cylinder space of the inner layer pipe is a main reaction area, perforated clapboards 7 are arranged in the middle and at the bottom of the main reaction area of the inner layer pipe, non-woven fabrics are laid on each perforated clapboard, the main reaction area of the inner layer pipe is divided into an upper layer and a lower layer by the perforated clapboards at the middle and at the bottom, namely, the area between the perforated clapboard at the middle and the perforated clapboard at the bottom is a lower layer packing layer, the area above the perforated clapboard at the middle is an upper layer packing layer, the perforated clapboard can be freely detached and installed, the packing in the upper space of the perforated clapboard plays a supporting role, and mass transfer between strains and substrates in sludge is prevented from being reduced due to most of sludge sinking to the bottom; the lower packing layer and the upper packing layer are both filled with biological nest type packing 10; the lower part of the main reaction zone is a funnel-shaped sludge hopper, the bottom end of the sludge hopper is provided with a water inlet 4 and a sludge circulation bottom outlet 6, the upper part of the main reaction zone is provided with a sludge circulation top inlet 11 and a water outlet 12, the water inlet 4 is connected with the water inlet tank 1 through the water inlet pump 3 and the water inlet pipe 2, the sludge circulation bottom outlet 6 is connected with the sludge circulation top inlet 11 through an external sludge pipeline, the water outlet 12 is connected with one end of the water outlet pipe 13, and the other end of the water outlet pipe 13 is immersed into the water in the water collecting tank 14, so that the device body is isolated from the air; and a gas discharge port 15 is arranged at the top of the main reaction zone and is connected with a water seal structure 17 through a gas discharge pipe 16.
The device of the invention is further characterized in that the whole cross section of the biological nest type filler 10 (see figure 2) is annular, the annular area is of a porous structure, a plurality of micro dentate structures 101 are distributed on the hole wall, and the outer wall of the annular area is provided with a gear-shaped structure 102. The structural characteristic of the biological nest type filler greatly improves the interception efficiency of the reactor to functional bacteria, in particular to Anammox bacteria with extremely slow growth; the porous structure of the filler is also beneficial to the discharge of the produced gas in the reaction process so as to avoid the blockage of the filler layer, and the slight scouring action generated by the gas discharge is beneficial to the updating of the biological membrane so as to keep the biological membrane at a better activity.
The device of the invention is further characterized in that the perforated partition plate is a detachable organic glass plate.
The biological nested filler can be obtained commercially, or can be designed and manufactured according to the structure in figure 2, and the whole biological nested filler is an annular porous structure. All lay the non-woven fabrics on the foraminiferous baffle of middle part and the foraminiferous baffle of bottom, the non-woven fabrics on the foraminiferous baffle of middle part is used for holding back the function fungus that its upper portion subsided and the function fungus that its lower part floated along with the bubble, and the non-woven fabrics on the foraminiferous baffle of bottom is held back and is subsided the function fungus that subsides because of gravity, avoids too much function fungus to flow into sludge hopper to improve the function fungus volume in main reaction zone, in order to guarantee device operation effect. The sludge hopper at the lower part of the device body is funnel-shaped, on one hand, the sludge hopper is beneficial to uniform water inflow at the bottom, on the other hand, the sludge hopper is beneficial to collecting culture sludge which leaks into the bottom due to the effects of gas production slight scouring and gravity sedimentation in a main reaction zone, after a period of operation, a large number of functional bacteria are contained in the sludge, the sludge enters the upper part of the main reaction zone from the sludge circulation bottom outlet 6 through the sludge top circulation inlet 11 via an external sludge pipeline, the entering sludge is subjected to gravity sedimentation to realize redistribution in the main reaction zone and re-film hanging on fillers, and the interception effect of the reactor on the functional bacteria is further strengthened.
The start of the oxygen-limited autotrophic nitrification-denitrification (OLAND) process adopts the conditions of normal temperature and low substrate (namely the temperature range is 15-25 ℃, the initial NH of water inflow 4 + The concentration range of-N is 20-30 mg/L), the starting process is more stable and safer, and the material consumption is greatly reduced.
The nitrification-denitrification (OLAND) process and the Anammox process related in the invention belong to the prior art, and the specific culture medium preparation and the like are operated according to a conventional method.
Example 1
The device (as shown in figure 1) of the embodiment is arranged in an environmental engineering laboratory of an energy-saving building in a new school district of the university of Hebei industry, and the environmental temperature of the laboratory is 15-25 ℃. The device and the method of the invention realize the rapid and effective start of the Anammox process under the normal temperature condition. The effective working volume of the device body was 2.6L, with a height to outside diameter ratio of 2.5. Biological nest type filler is filled to the device body, and biological nest type filler adopts the polyethylene to make, and the cross section of this filler is whole to be cyclic annularly, is porous structure in the annular region, and concrete structure is as shown in figure 2, and biological nest type filler has 19 holes in this embodiment, can improve the efficiency of holding back of reactor to the function fungus, easily reacts gaseous discharge of production simultaneously.
The specific operation method comprises the following steps:
(1) The inoculated sludge is aerobic activated sludge of an aeration tank of a certain sewage treatment plant, and the sludge inoculation amount is 2L. MLSS (mixed liquor suspended solid concentration) and MLVSS (mixed liquor volatile suspended solid concentration) of the sludge mixed liquor at the time of inoculation were 2284mg/L and 1935mg/L, respectively.
(2) Prepared according to the OLAND culture medium (see Kuai L P, versraete W. Anammonium removal by the Oxygen-Limited aureophile diagnosis-diagnosis system, applied and Environmental Microbiology,1999,39 (7): 13-21.) simulated wastewater, total nitrogen in the feed waterAmmonia nitrogen supply and initial NH of inlet water 4 + N concentration 20mg/L, DO 0.5-1.0mg/L, hydraulic Retention Time (HRT) 2 days. And (3) starting the OLAND process by adopting a continuous water inlet mode operation device under the condition of normal temperature (15-25 ℃). When the total nitrogen of the effluent is less than 5mg/L, the ammonia Nitrogen (NH) in the influent water can be improved 4 + -N) concentration. After 30 days of operation, the ammonia nitrogen concentration of the inlet water of the device is gradually increased to 100mg/L, and the removal rate of the ammonia nitrogen and the total nitrogen in the outlet water is stabilized to be more than 90 percent. The device is shown to realize the start of the OLAND process under the conditions of normal temperature and low substrate to obtain the OLAND biomembrane, wherein a certain amount of Anamox bacteria is pre-enriched.
(3) Anammox bacteria are further enriched by the cultured OLAND sludge under the normal temperature condition (15-25 ℃) so as to start the Anammox process. Wastewater was simulated with Anammox medium configuration (see Dapena-Mora, a., van Hulle, s.w.h., luis, campos j., mendez, r.vanroleghem, p.a.jetten, m.2004. Environment of Anammox biomass free activated sludge and modular measures.j.chem.technol.79) and the DO in the wastewater was removed by exposure to nitrogen for 5 min. The reactor continued to maintain a continuous water feed mode. The reactor and the pipeline interface are sealed, so that the anaerobic environment required by the Anammox reaction is ensured. The total nitrogen in the feed water is provided by ammonia nitrogen and nitrite nitrogen, wherein NH 4 + -N and NO 2 - The initial concentration of-N was 70mg/L, and HRT was controlled at 2d. The device is operated for 10 days at normal temperature (15-25 ℃), 70mg/L NH in the inlet water 4 + -N and NO 2 - The N is synchronously removed by the reactor, and water NH is discharged 4 + -N and NO 2 - The removal rates of-N are 95% and 100%, respectively, and the transition from OLAND biomembrane to Anammox biomembrane is realized, which indicates that the anaerobic ammonia oxidation process is successfully started at normal temperature. Running for 50 days, feeding water NH 4 + -N and NO 2 - The N concentration is gradually increased to 230 mg/L and 260mg/L, the removal rate of the N concentration and the removal rate of the N concentration are both more than 95%, the Anramox bacteria are enriched in enough quantity, and the Anramox process shows stable denitrification performance after being successfully started under the normal temperature condition.
In the embodiment, the starting method and the starting device can realize the quick starting of the anaerobic ammonia oxidation process, the starting time is about forty days, compared with the conventional anaerobic ammonia oxidation starting process, the starting time is greatly shortened, the normal-temperature starting can be realized, the energy consumption caused by temperature control in the prior art is greatly saved, and the device can stably run at the normal temperature after being started and is safer and more reliable.
In addition, the hydraulic retention time was controlled to 2 days in the examples, which greatly increased the treatment capacity of sewage treatment using the start-up method.
Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are illustrative and not restrictive, and that those skilled in the art may make changes, modifications, substitutions and alterations to the above embodiments without departing from the scope of the present invention.
Nothing in this specification is said to apply to the prior art.
Claims (2)
1. A method for quickly starting up anaerobic ammonia oxidation at normal temperature comprises the following steps:
1) Inoculating common activated sludge into the reactor, wherein the common activated sludge is aerobic activated sludge, nitrified sludge or denitrifying sludge, the suspended solid concentration MLSS of the inoculated sludge mixed solution is 2000-3000mg/L, and MLVSS/MLSS =0.6-0.85;
2) Starting an oxygen-limited autotrophic nitrification-denitrification (OLAND) process under the condition of normal temperature and low substrate: preparing simulated wastewater according to an OLAND culture medium, continuously flowing into a reactor through a water inlet pump, and feeding initial NH 4 ⁺ -N concentration is 20-30mg/L, dissolved oxygen is controlled at 0.5-1mg/L, hydraulic retention time is 2-4 days, and NH in inlet water is gradually increased 4 ⁺ -N concentration, NH when influent 4 ⁺ -N concentration is increased to 100mg/L, the removal rate of ammonia nitrogen and total nitrogen in effluent is above 90%, the OLAND process is started at normal temperature, OLAND biomembranes are obtained, and pre-enrichment of Anammox (Anamox) bacteria is realized;
3) Starting an Anammox process under the condition of normal temperature: prepared according to Anammox culture mediumSetting simulated waste water, continuous water feeding mode for reactor operation, NH 4 ⁺ -N and NO 2 5363 initial concentration of zxft 5363-N is 60-80 zxft 3242/L, the hydraulic retention time is 2-4 days by gradually increasing NH in the feed water 4 ⁺ -N and NO 2 ⁻ -N concentration, gradually enriching Anamox bacteria, and producing NH in water 4 ⁺ -N and NO 2 5363 the removal rate of ⁻ -N is more than 95%, the transition from OLAND biomembrane to Anammox biomembrane is realized, and the anaerobic ammonia oxidation process is successfully started at normal temperature.
2. A normal-temperature anaerobic ammonia oxidation quick start device is characterized in that the device is constructed on the basis of a fixed bed reactor by applying the start method of claim 1 and comprises a device body, a water inlet tank, a water seal structure and a water collecting tank,
the device body is a cylinder and comprises an inner layer pipe and an outer layer pipe, an air interlayer is formed between the inner layer pipe and the outer layer pipe, the cylinder space of the inner layer pipe is a main reaction area, perforated partition boards are arranged at the middle part and the bottom part of the main reaction area of the inner layer pipe, non-woven fabrics are laid on each perforated partition board, the main reaction area of the inner layer pipe is divided into an upper layer and a lower layer by the perforated partition boards at the middle part and the bottom part, namely, an area between the middle perforated partition board and the bottom perforated partition board is a lower layer packing layer, and an area above the middle perforated partition board is an upper layer packing layer; biological nest type fillers are filled in the lower layer filler layer and the upper layer filler layer; the lower part of the main reaction zone is a funnel-shaped sludge hopper, the bottom end of the sludge hopper is provided with a water inlet and a sludge circulation bottom outlet, the upper part of the main reaction zone is provided with a sludge circulation top inlet and a water outlet, the water inlet is connected with a water inlet tank through a water inlet pump and a water inlet pipe, the sludge circulation bottom outlet is connected with the sludge circulation top inlet through an external sludge pipeline, the water outlet is connected with one end of a water outlet pipe, and the other end of the water outlet pipe is immersed in the water collecting tank, so that the device body is isolated from the air; the top of the main reaction zone is provided with a gas discharge port which is connected with a water seal structure through an exhaust pipe; the perforated partition plate is a detachable organic glass plate; the whole cross section of the biological nest type filler is annular, the annular area is of a porous structure, a plurality of micro dentate structures are distributed on the hole wall, and the outer wall of the annular area is provided with a gear-shaped structure.
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| CN107162196A (en) * | 2017-07-10 | 2017-09-15 | 北京工业大学 | Short distance nitration Anammox coupling denitrification handles the method and apparatus of city domestic sewage |
| CN107523474A (en) * | 2017-10-23 | 2017-12-29 | 江苏艾特克环境工程设计研究院有限公司 | The culture apparatus and cultural method of a kind of anaerobic ammonia oxidizing bacteria |
| CN108178294B (en) * | 2017-11-28 | 2023-04-21 | 郑州轻工业学院 | Method for synchronously starting low-matrix anaerobic ammonia oxidation process in series |
| CN109485153A (en) * | 2018-12-19 | 2019-03-19 | 清华-伯克利深圳学院筹备办公室 | A kind of biofilm reactor and method of wastewater efficient denitrogenation |
| CN110104769B (en) * | 2019-04-22 | 2022-05-27 | 杭州师范大学 | Method for domesticating and starting high-load mainstream anaerobic ammonia oxidation reactor |
| CN111439858A (en) * | 2020-04-03 | 2020-07-24 | 镇江江南化工有限公司 | Method for comprehensively treating glyphosate organic phosphorus wastewater |
| CN113860488B (en) * | 2021-10-08 | 2023-07-11 | 广东中微环保生物科技有限公司 | Anaerobic ammonia oxidizing bacteria particle culture method and device |
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