CN106513068A - Solution used for bonding and surface modification of micro-fluidic chips made from polymer and application thereof - Google Patents
Solution used for bonding and surface modification of micro-fluidic chips made from polymer and application thereof Download PDFInfo
- Publication number
- CN106513068A CN106513068A CN201610941525.4A CN201610941525A CN106513068A CN 106513068 A CN106513068 A CN 106513068A CN 201610941525 A CN201610941525 A CN 201610941525A CN 106513068 A CN106513068 A CN 106513068A
- Authority
- CN
- China
- Prior art keywords
- bonding
- chip
- solution
- micro
- modified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920000642 polymer Polymers 0.000 title abstract description 8
- 238000012986 modification Methods 0.000 title abstract description 6
- 230000004048 modification Effects 0.000 title abstract description 6
- 230000003139 buffering effect Effects 0.000 claims abstract 3
- 238000000034 method Methods 0.000 claims description 37
- 239000000463 material Substances 0.000 claims description 15
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims description 12
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 11
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 11
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 claims description 8
- 229910000077 silane Inorganic materials 0.000 claims description 8
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 7
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 6
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 6
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims description 6
- 229910052736 halogen Inorganic materials 0.000 claims description 6
- 150000002367 halogens Chemical class 0.000 claims description 6
- 230000002209 hydrophobic effect Effects 0.000 claims description 6
- 150000001335 aliphatic alkanes Chemical group 0.000 claims description 5
- JRZJOMJEPLMPRA-UHFFFAOYSA-N 1-nonene Chemical compound CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 claims description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 4
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical compound CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 claims description 4
- CSRZQMIRAZTJOY-UHFFFAOYSA-N trimethylsilyl iodide Chemical compound C[Si](C)(C)I CSRZQMIRAZTJOY-UHFFFAOYSA-N 0.000 claims description 4
- 125000005647 linker group Chemical group 0.000 claims description 3
- LIKMAJRDDDTEIG-UHFFFAOYSA-N 1-hexene Chemical compound CCCCC=C LIKMAJRDDDTEIG-UHFFFAOYSA-N 0.000 claims description 2
- KWKAKUADMBZCLK-UHFFFAOYSA-N 1-octene Chemical compound CCCCCCC=C KWKAKUADMBZCLK-UHFFFAOYSA-N 0.000 claims description 2
- BKOOMYPCSUNDGP-UHFFFAOYSA-N 2-methylbut-2-ene Chemical group CC=C(C)C BKOOMYPCSUNDGP-UHFFFAOYSA-N 0.000 claims description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 239000004793 Polystyrene Substances 0.000 claims description 2
- KERCGYZCAYGEQW-UHFFFAOYSA-N [SiH4].CIC Chemical compound [SiH4].CIC KERCGYZCAYGEQW-UHFFFAOYSA-N 0.000 claims description 2
- 229910052794 bromium Inorganic materials 0.000 claims description 2
- IYYIVELXUANFED-UHFFFAOYSA-N bromo(trimethyl)silane Chemical compound C[Si](C)(C)Br IYYIVELXUANFED-UHFFFAOYSA-N 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 229910052801 chlorine Inorganic materials 0.000 claims description 2
- QABCGOSYZHCPGN-UHFFFAOYSA-N chloro(dimethyl)silicon Chemical compound C[Si](C)Cl QABCGOSYZHCPGN-UHFFFAOYSA-N 0.000 claims description 2
- 230000008020 evaporation Effects 0.000 claims description 2
- 238000001704 evaporation Methods 0.000 claims description 2
- 229910052731 fluorine Inorganic materials 0.000 claims description 2
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 2
- 125000002346 iodo group Chemical group I* 0.000 claims description 2
- TVMXDCGIABBOFY-UHFFFAOYSA-N octane Chemical compound CCCCCCCC TVMXDCGIABBOFY-UHFFFAOYSA-N 0.000 claims description 2
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 2
- -1 polydimethylsiloxane Polymers 0.000 claims description 2
- 229920002223 polystyrene Polymers 0.000 claims description 2
- RYPYGDUZKOPBEL-UHFFFAOYSA-N trichloro(hexadecyl)silane Chemical compound CCCCCCCCCCCCCCCC[Si](Cl)(Cl)Cl RYPYGDUZKOPBEL-UHFFFAOYSA-N 0.000 claims description 2
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 claims 1
- 239000004205 dimethyl polysiloxane Substances 0.000 claims 1
- 239000002861 polymer material Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 13
- 239000000203 mixture Substances 0.000 abstract description 9
- 230000004907 flux Effects 0.000 abstract description 4
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 19
- 230000008569 process Effects 0.000 description 18
- 239000011259 mixed solution Substances 0.000 description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 230000008901 benefit Effects 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 230000008021 deposition Effects 0.000 description 4
- 239000003292 glue Substances 0.000 description 4
- 238000011031 large-scale manufacturing process Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000002480 mineral oil Substances 0.000 description 4
- 235000010446 mineral oil Nutrition 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 238000004026 adhesive bonding Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 125000004836 hexamethylene group Chemical class [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 241001282153 Scopelogadus mizolepis Species 0.000 description 1
- OATWCNSODQAOQC-UHFFFAOYSA-N [SiH4].Br Chemical compound [SiH4].Br OATWCNSODQAOQC-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000002346 layers by function Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- 239000012756 surface treatment agent Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Lining Or Joining Of Plastics Or The Like (AREA)
Abstract
The invention discloses a solution used for bonding and surface modification of micro-fluidic chips made from polymer, and the solution comprises a solution buffering composition, a bonding composition and a surface modification composition; the solution buffering composition, the bonding composition and the surface modification composition are mutually soluble, reaction doesn't occur, and the chip cannot be corroded simultaneously. The solution used for bonding and surface modification of the micro-fluidic chips made from the polymer is suitable for large scale chip bonding, and the production flux is high; the bonding dead zones are few, and the strength is high; the equipment is simple, and the production cost is low; and after bonding, the pipeline deformation is small.
Description
Technical field
The present invention relates to a kind of micro-fluidic chip field, and in particular to a kind of micro-fluidic chip key for polymerizable material
Close the solution and its application being modified with surface.
Background technology
Microfluidic chip technology feature is, based on micro-processing technology, will to realize sample feeding, mixing, reaction and detect
Etc. function structure assembly in several square centimeters of chip.In field of biochemistry detection, microfluidic chip technology has logical
Measure high high, detection sensitivity, low cost and be easy to the potential advantages such as automatization, thus with very wide application prospect.
Chip bonding is an important step in micro-fluidic chip process of manufacture.Chip bonding is referred to by micro-
The structures such as micro- raceway groove, micro- hole and through hole that processing is obtained, are packaged into closed microchannel, microcavity body and sample import and export etc..With
Polymer has thermocompression bonding, mono-/bis-face glue bonding and organic solution auxiliary for the conventional bonding method of the micro-fluidic chip of material
Bonding.
(1) mode of thermocompression bonding is adapted to the bonding between polymer chip, is by applying certain temperature to bonding face
Degree, temperature close on or reach polymer glass temperature, allow bonding face to reach the state of softening, while apply certain pressure,
Allow bonding face that fusion occurs, complete chip bonding.Thermocompression bonding has bond strength high, without the need for extra auxiliary reagent, thus
Noresidue in pipeline.But being disadvantageous in that needs higher temperature;And due to the problem of heat transfer, often thermocompression bonding needs
Longer time;Stress control also has certain requirement.
(2) glue bonding in mono-/bis-face referred to and acted on bonding face using mono-/bis-face glue, the method for making chip bonding.It is this
Requirement of the method to mono-/bis-face glue is relatively more, and the compatibility of the intensity, biological sample that are such as bonded, hydrophilic and hydrophobic etc. are required.This
Plant method bonding process simple, it is easy to large-scale production.But compared with other bonding patterns, bond strength is not often high;Glue
Composition is easily formed to biochemical reaction and is disturbed;Most of glue all easily produces background fluorescence, optical detection is formed and is disturbed.
(3) organic solution auxiliary bonding is that have infiltration, the effect for softening to polymer chip substrate using organic solution,
So that bonding face permeates each other in bonding process, after organic solvent volatilization, bonding face is hardened again and is bonded together, and is completed
Bonding.Organic solution auxiliary bonding is high with bond strength, and bonding blind area is few, and bonding speed is fast, low cost and remains without reagent
The advantages of, it is particularly suitable for large-scale production process.But traditional organic solvent easily corrodes chip structure so that chip structure is sent out
Raw deformation, affects the work of chip.
It is another important link in micro-fluidic chip production that surface is modified.Modified the referring in surface is keeping chip material
On the premise of originality energy, give its surface new performance.Due to the characteristic of chip base material, such as hydrophobe characteristic, often without
Method meets requirement, so that surface is carried out to its pipe surface being modified, the conventional process for modifying surface of micro-fluidic chip has wet
Method is modified and vapour deposition both approaches.
(1) it is wet-process modified by liquid reagent process chip pipeline, change its hydrophobe characteristic.This method has very extensive
Application, especially in micro-fluidic chip academic research field, principle is by the liquid reagent micro-fluidic chip made of injection
It is interior so as to hydrophilic or hydrophobic functional layer is formed on inner-walls of duct, reach the purpose of modification surface characteristic.This method has
Reagent cost is low, be adapted to manual operation the advantages of.But in large-scale production, wet treatment needs for reagent to lead to every chip
Pipeline in, this just becomes very poorly efficient, limits production flux.
(2) vapour deposition be using the physics, chemical process occurred in gas phase, chip pipe surface formed feature or
Ornamental metal, nonmetallic or compound coat.Vapour deposition can process large batch of chip simultaneously, thus relatively be adapted to big
Scale processes.But as vapour deposition generally requires the environment of vacuum, therefore equipment cost is very high.Meanwhile, to control
The modified concordance in good surface everywhere, it is also desirable to very complicated control.
The content of the invention
The present invention is in order to solve the above problems, there is provided a kind of micro-fluidic chip bonding for polymerizable material is changed with surface
The solution of property, the solution include following components:Buffer components, linked component and surface-modifying component, the buffer components, key
Charge-coupled point dissolves each other with surface-modifying component, and does not react, while chip will not be corroded;The buffer components control key is charge-coupled
Divide the softening rate to chip so that can realize that chip bonding softens chip again not too much, the buffer components are molten with described
The volume range of liquid is 20%~90%, it is therefore preferable to 30%~80%, more preferably 40%-70%;The bonding group
Divide and cause chip surface to become to soften, after its evaporation, bonding, the linked component and the solution are realized in chip bonding face
Volume range is 10%~90%, it is therefore preferable to 20%~80%, more preferably 30%-60%;It is modified with the surface
Component is hydrophobic silane, and its molecular structure isWherein halogen can be F, Cl, Br, I atom, halogen
Prime number amount is 1 to 3, and the carbon number of alkane chain is 1 to 20, and the H atom of alkane chain can be partly or entirely by halogen substiuted;Institute
The volume range that surface-modifying component is stated with the solution is 0%~20%;Preferably 5%~15%, more preferably
8%-12%.
In one embodiment, buffer components are selected from pentane, hexane, octane, amylene, hexene, octene and nonene one
Plant or various.
In one embodiment, linked component is in the middle of acetone, dichloromethane, chloroform, toluene, benzene
Plant or various.
In one embodiment, surface-modifying component is selected from dimethylchlorosilane, trim,ethylchlorosilane, diformazan bromide
Silane, bromotrimethylsilane, dimethyl iodine silane, Iodotrimethylsilane, hexadecyl trichlorosilane, trichlorine (octyl group) silane and
One or more in the middle of trichlorine (1H, 1H, 2H, 2H- perfluoro capryl) silane.
In one embodiment, polymerizable material be Merlon, polymethyl methacrylate, polydimethylsiloxanes
Alkane or polystyrene.
In one embodiment, the present invention is provided a kind of micro-fluidic chip bonding for polymerizable material and is changed with surface
Property method, the method comprising the steps of:
Step 1:The solution that the above-mentioned micro-fluidic chip bonding for polymerizable material of configuration is modified with surface;
Step 2:The bonding face that needs of the micro-fluidic chip of polymerizable material is fully infiltrated into configured in step 1 molten
In liquid;
Step 3:By the chip bonding infiltrated in step 2 in the face of neat assembling, be bonded, control bonding temperature 10~
120 DEG C, bonding time is controlled at 10 seconds~20 minutes;
Step 4:After the completion of bonding, the solution remained in chip is blown off with dry air.Beneficial effects of the present invention:
1. produce flux high, be adapted to monster chip bonding.As, in monster chip production process, surface is modified past
It is past more time-consuming.The present invention is bonded polymer chip and surface the two important processing environments that are modified unite two into one,
Surface is carried out while bonding to be modified, the wet-process modified problem for being not suitable for large-scale production process is not only solved, while also
Eliminating individually carries out the modified production link of chip surface, therefore greatly improves the flux of production.
2 bonding blind areas are few, and intensity is high.The method of the bonding chip of the present invention is that the side of bonding is aided in based on organic solution
Method, thus also inherit its bonding blind area it is few, the high advantage of bond strength.
3 equipment are simple, low production cost.The present invention by it is wet-process modified be integrated in organic solution auxiliary bonding during,
Thus required device systems are fairly simple.Simultaneously as wet-process modified and organic solution auxiliary bonding all has production cost
Low advantage, thus the present invention similarly has the advantages that low production cost.
After 4 bondings, the deformation of pipeline is little.Due to containing buffer components in the present invention, chip pipeline configuration can be formed and be protected
Shield, reduces impact of the linked component to pipeline form so that the deformation of pipeline is little after bonding.
Description of the drawings
For the technical scheme being illustrated more clearly that in the embodiment of the present application, below by to be used needed for embodiment
Accompanying drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments described in the application, right
For those of ordinary skill in the art, on the premise of not paying creative work, can be obtaining which according to these accompanying drawings
Its accompanying drawing.
The Contact-angle measurement figure of PMMA chip surfaces of the Fig. 1 to process without mixed solution in embodiment 1;
The Contact-angle measurement figure of PMMA chip surfaces of the Fig. 2 to process through mixed solution in embodiment 1;
The result figure of drop formation chip production drops of the Fig. 3 to be bonded by mixed solution A in embodiment 2;With
The result figure of drop formation chip production drops of the Fig. 4 to be bonded by mixed solution B in embodiment 2.
Specific embodiment
In order that art technology field personnel more fully understand the technical scheme in the application, below in conjunction with following knot
The invention will be further described to close embodiment, it is clear that described embodiment is only some embodiments of the present application, and not
It is whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not before creative work is made
The all other embodiment for being obtained is put, should all belong to the scope of the application protection.Below in conjunction with the accompanying drawings and instantiation
The invention will be further described.
Change of one mixed solution of embodiment to PMMA material hydrophilic and hydrophobics
Step one:The PMMA chips of two panels surfacing are taken, length and width are respectively about 3cm, thickness 5mm;It is positioned in pure water,
Cleaned with supersonic cleaning machine, and dried.
Step 2:Configuration 1ml mixed solutions, wherein 500 μ l of buffer components, 400 μ l of linked component, surface-modifying component
100μl.Wherein, buffer components composition is normal hexane, and linked component is chloroform, surface-modifying component be trichlorine (1H, 1H,
2H, 2H- perfluoro capryl) silane, its structural formula isThen stir.
Step 3:Oxygen plasma process, process time 60 seconds are carried out to the two panels PMMA chip in step one.
Step 4:Two panels PMMA chip is kept flat, by 1ml mixed solutions uniform application a piece of PMMA chip lists wherein
Face, another is not processed.Two panels chip is each positioned in ventilated container, in container, temperature is set to 50 DEG C.Treat 30 points
Take out after clock, and the solution that will be remained on processed chip is blown away with dry air, stand 10 minutes.
Step 5:Two panels chip is positioned on contact angle measurement, respectively the distilled water water droplet of 2 μ l of Deca, is clapped after 5 seconds
Contact angle photo is taken the photograph, and is measured with goniometry.Untreated PMMA chip surfaces contact angle is 72 °, as a result such as Fig. 1.It is mixed
The PMMA chip surfaces contact angle for closing solution process is 113 °, and contact angle increased 41 °, as a result such as Fig. 2.
Embodiment two is bonded the generation that PC chips realize drop using mixed solution
Step one:The PC chip two panels with cross pipeline is taken, chip length and width are 3cm, and pipeline is deep 100 μm, wide 100 μ
M, four branches ends of cross pipeline have been all connected with through hole, and through hole can be used by the gateway as fluid.Separately take and do not contain pipe
The PC chips of road and through-hole structure, length and width are 3cm.Four chips are cleaned with supersonic cleaning machine, and is dried.
Step 2:Configuration mixed solution A 1ml, including 500 μ l of buffer components, 400 μ l of linked component, surface-modifying component
100μl.Wherein, buffer components composition is 1- hexenes, and linked component is dichloromethane, surface-modifying component be trichlorine (1H, 1H,
2H, 2H- perfluoro capryl) silane.Configure the mixed solution B1ml without surface treatment component, including 600 μ l of buffer components, bonding
400 μ l of component.Wherein, buffer components are 1- hexenes, and linked component is dichloromethane.
Step 3:Oxygen plasma process, process time 60 seconds are carried out to four PC chips in step one.
Step 4:The PC chips that two panels does not contain pipeline configuration are kept flat, by 1ml mixed solution As uniform application wherein
A piece of PC chip surfaces, mixed solution B uniform applications are on another surface.After standing 10 seconds, by other two panels PC chip belt pipe
Road structure faces down, and the face for smearing mixed solution with other two panels PC chip closely forces together, pressure is 50N.Keep
Pressure is simultaneously placed them in ventilated container, and in container, temperature is set to 80 DEG C.Took out after ten minutes.Now chip has been
Bonding is finished, and obtains two complete chips.
Step 5:The solution remained in chip is blown away with dry air, 10 minutes are stood.
Step 6:Utilizing syringe to inject water and mineral oil in two panels chip respectively is used to generate drop.Wherein, cross
One pipeline injection water of pipeline, flow velocity is 800 μ l/h, and its two adjacent pipeline injection mineral oil, flow velocity are 600 μ l/
H, water and mineral oil converge at cross, then flow to remaining pipeline together, and flow out from outlet.In such cases, drop
Generation need pipeline that there is certain hydrophobicity.The interaction process of cross intersection water and mineral oil is used under the microscope
High speed camera is recorded, as a result such as Fig. 3, Fig. 4.With the chip of mixed solution A bonding due to containing surface treatment agent, inner-walls of duct
It is with hydrophobic, therefore, it is possible to generate the drop of size uniformity.And the chip being bonded with mixed solution B does not carry out pipeline
Surface is modified, and unmodified pipeline hydrophobicity not enough, thus cannot generate drop.Whole process chip sealing is good, and mixed
The chip of conjunction solution A bonding can carry out surface and be modified to pipeline, illustrate that the present invention is provided simultaneously with bonding chip and is modified with surface
Ability.Jing is measured, and the chip bonding intensity of mixed solution A bonding is up to 113N/cm2, the chip bonding of mixed solution B bondings
Intensity is up to 152N/cm2。
It should be understood that the present invention for disclosing is not limited only to specific method, scheme and the material for describing, because these
Equal alterable.It will also be understood that purpose of the terminology used here just for the sake of the specific embodiment scheme of description, rather than
It is intended to limit the scope of the present invention, the scope of the present invention is limited solely by appended claim.
Those skilled in the art will also be appreciated that or be able to confirm that and use no more than normal experiment, institute herein
Many equivalents of the specific embodiment of the present invention for stating.These equivalents are also contained in appended claim.
Claims (6)
1. be used for polymerizable material micro-fluidic chip bonding with surface be modified solution, it is characterised in that the solution include with
Lower component:Buffer components, linked component and surface-modifying component, the buffer components, linked component and surface-modifying component are mutual
It is molten, and do not react, while chip will not be corroded;
The buffer components control softening rate of the linked component to chip so that can realize that chip bonding softens core again not too much
Piece, it is 20%~90% with the volume range of the solution that the buffer components are, it is therefore preferable to 30%~80%, more preferably
Ground is 40%-70%;
The linked component causes chip surface to become to soften, and after its evaporation, bonding, the bonding group are realized in chip bonding face
The volume range with the solution is divided to be 10%~90%, it is therefore preferable to 20%~80%, more preferably 30%-60%;
With
The surface-modifying component is hydrophobic silane, and its molecular structure isWherein halogen can be
F, Cl, Br, I atom, halogen quantity are 1 to 3, and the carbon number of alkane chain is 1 to 20, the H atom of alkane chain can part or
All by halogen substiuted;The surface-modifying component is 0%~20% with the volume range of the solution;Preferably 5%
~15%, more preferably 8%-12%.
2. the solution that the micro-fluidic chip bonding according to claim 1 is modified with surface, it is characterised in that the buffering group
It is divided into selected from pentane, hexane, one or more of octane, amylene, hexene, octene and nonene.
3. the solution that the micro-fluidic chip bonding according to claim 1 is modified with surface, it is characterised in that the bonding group
One or more be divided in the middle of acetone, dichloromethane, chloroform, toluene, benzene.
4. the solution that the micro-fluidic chip bonding according to claim 1 is modified with surface, it is characterised in that the surface changes
Property component be selected from dimethylchlorosilane, trim,ethylchlorosilane, dimethyl bromo-silicane, bromotrimethylsilane, dimethyl iodine silane,
Iodotrimethylsilane, hexadecyl trichlorosilane, trichlorine (octyl group) silane and trichlorine (1H, 1H, 2H, 2H- perfluoro capryl) silane
Central one or more.
5. the solution that the micro-fluidic chip bonding according to claim 1 is modified with surface, it is characterised in that the polymer
Material is Merlon, polymethyl methacrylate, polydimethylsiloxane or polystyrene.
6. a kind of micro-fluidic chip for polymerizable material is bonded and surface modifying method, it is characterised in that methods described includes
Following steps:
Step 1:The solution of configuration claim 1;
Step 2:The bonding face that needs of the micro-fluidic chip of polymerizable material is fully infiltrated into the solution for being configured in step 1
In;
Step 3:By the chip bonding infiltrated in step 2 in the face of neat assembling, be bonded, bonding temperature is controlled at 10~120 DEG C,
Bonding time is controlled at 10 seconds~20 minutes;
Step 4:After the completion of bonding, the solution remained in chip is blown off with dry air.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610941525.4A CN106513068B (en) | 2016-10-25 | 2016-10-25 | The solution and its application that micro-fluidic chip bonding for polymerizable material is modified with surface |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610941525.4A CN106513068B (en) | 2016-10-25 | 2016-10-25 | The solution and its application that micro-fluidic chip bonding for polymerizable material is modified with surface |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106513068A true CN106513068A (en) | 2017-03-22 |
CN106513068B CN106513068B (en) | 2018-10-30 |
Family
ID=58293061
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610941525.4A Active CN106513068B (en) | 2016-10-25 | 2016-10-25 | The solution and its application that micro-fluidic chip bonding for polymerizable material is modified with surface |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106513068B (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107262173A (en) * | 2017-08-03 | 2017-10-20 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | PDMS micro-fluidic chips and the method that PDMS micro-fluidic chips are prepared based on wet etching |
CN111167526A (en) * | 2018-11-13 | 2020-05-19 | 中国科学院大连化学物理研究所 | Sealing method of acrylic glass microfluidic chip |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002033410A2 (en) * | 2000-10-16 | 2002-04-25 | Board Of Trustees Of The University Of Arkansas | Microvolume immunoabsorbant assays with amplified electrochemical detection |
US20020153251A1 (en) * | 1999-02-03 | 2002-10-24 | Alexander Sassi | Multichannel control in microfluidics |
CN101633742A (en) * | 2009-09-01 | 2010-01-27 | 博奥生物有限公司 | Amino plastic substrate, and preparation method and application thereof |
CN101643701A (en) * | 2009-07-23 | 2010-02-10 | 清华大学 | Cell sorter micro-fluidic chip based on immunomagnetic separation technology and application thereof in aspect of enrichment of rare cells |
CN101914435A (en) * | 2010-05-24 | 2010-12-15 | 博奥生物有限公司 | Microtube device and using method thereof |
CN103344464A (en) * | 2013-06-08 | 2013-10-09 | 南京理工大学 | Micro-fluidic agglutinin chip for glycosyl separation, and preparation method thereof |
CN105363503A (en) * | 2015-11-02 | 2016-03-02 | 华东理工大学 | Multicomponent micro droplet microfluidic chip and processing method thereof |
-
2016
- 2016-10-25 CN CN201610941525.4A patent/CN106513068B/en active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020153251A1 (en) * | 1999-02-03 | 2002-10-24 | Alexander Sassi | Multichannel control in microfluidics |
WO2002033410A2 (en) * | 2000-10-16 | 2002-04-25 | Board Of Trustees Of The University Of Arkansas | Microvolume immunoabsorbant assays with amplified electrochemical detection |
CN101643701A (en) * | 2009-07-23 | 2010-02-10 | 清华大学 | Cell sorter micro-fluidic chip based on immunomagnetic separation technology and application thereof in aspect of enrichment of rare cells |
CN101633742A (en) * | 2009-09-01 | 2010-01-27 | 博奥生物有限公司 | Amino plastic substrate, and preparation method and application thereof |
CN101914435A (en) * | 2010-05-24 | 2010-12-15 | 博奥生物有限公司 | Microtube device and using method thereof |
CN103344464A (en) * | 2013-06-08 | 2013-10-09 | 南京理工大学 | Micro-fluidic agglutinin chip for glycosyl separation, and preparation method thereof |
CN105363503A (en) * | 2015-11-02 | 2016-03-02 | 华东理工大学 | Multicomponent micro droplet microfluidic chip and processing method thereof |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107262173A (en) * | 2017-08-03 | 2017-10-20 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | PDMS micro-fluidic chips and the method that PDMS micro-fluidic chips are prepared based on wet etching |
CN111167526A (en) * | 2018-11-13 | 2020-05-19 | 中国科学院大连化学物理研究所 | Sealing method of acrylic glass microfluidic chip |
CN111167526B (en) * | 2018-11-13 | 2021-05-25 | 中国科学院大连化学物理研究所 | Sealing method of acrylic glass microfluidic chip |
Also Published As
Publication number | Publication date |
---|---|
CN106513068B (en) | 2018-10-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Lin et al. | Magnetically induced low adhesive direction of nano/micropillar arrays for microdroplet transport | |
CN104672962B (en) | A kind of inorganic matter super hydrophobic coating and application thereof | |
CN106644189B (en) | Pliable pressure sensor and preparation method thereof | |
Yin et al. | Magnetic-directed assembly from Janus building blocks to multiplex molecular-analogue photonic crystal structures | |
Kaufmann et al. | 'Sandwich'microcontact printing as a mild route towards monodisperse janus particles with tailored bifunctionality | |
EP2155620B1 (en) | Preparation of super water repellent surface | |
Su et al. | One-step bonding and hydrophobic surface modification method for rapid fabrication of polycarbonate-based droplet microfluidic chips | |
CN105347690B (en) | Recoverable coating material of a kind of transparent and ultra-hydrophobicity and preparation method thereof | |
CN104002474A (en) | Preparation method and application of super-hydrophobic and adhesion-adjustable surface with micro-nano composite structure | |
CN106513068B (en) | The solution and its application that micro-fluidic chip bonding for polymerizable material is modified with surface | |
CN106232696A (en) | There is the fluid control films of water-wetted surface, its manufacture method and the method for cleaning structure surface | |
KR20090108371A (en) | Microfluidic circuit element comprising microfluidic channel with nano interstices and fabrication thereof | |
CN101000290B (en) | Sample enrichment chip, manufacturing method | |
CN104947169A (en) | Preparation method of super-hydrophobic silicon dioxide film | |
CN102580799A (en) | Machining method of micro-drop and micro-fluidic control chip | |
CN107442191A (en) | A kind of centrifugal type microfludic chip for Water-In-Oil drop formation | |
CN1764842B (en) | Microchip, nucleic acid extracting kit, and nucleic acid extracting method | |
CN108579828A (en) | A kind of Surface modification of microfluidic chip method that flow velocity is controllable | |
Chen et al. | Robust raspberry-like all-polymer particles for the construction of superhydrophobic surface with high water adhesive force | |
Li et al. | Empowering microfluidics by micro-3D printing and solution-based mineral coating | |
Nabesawa et al. | Low-pressure plasma-etching of bulk polymer materials using gas mixture of CF4 and O2 | |
CN111359683A (en) | Gradient micro-fluid channel for unpowered liquid drop transportation and preparation method thereof | |
CN107262173A (en) | PDMS micro-fluidic chips and the method that PDMS micro-fluidic chips are prepared based on wet etching | |
Yin et al. | Multilayer patterning technique for micro-and nanofluidic chip fabrication | |
CN106563626A (en) | Preparation method of super-hydrophobic resistance-reducing coating |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP01 | Change in the name or title of a patent holder |
Address after: 100084 Tsinghua Yuan, Beijing, Haidian District Co-patentee after: Beijing Xinyi Biotechnology Co., Ltd. Patentee after: Tsinghua University Address before: 100084 Tsinghua Yuan, Beijing, Haidian District Co-patentee before: Beijing Tianjian Wellcome Biotechnology Co. Ltd. Patentee before: Tsinghua University |
|
CP01 | Change in the name or title of a patent holder |