CN106509895A - 一种益生菌保健品及其制备方法 - Google Patents
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Abstract
本发明提供了一种益生菌保健品,由下列重量份的原料制成:半叶马尾藻提取物60‑100份、羽脉野扇花提取物40‑80份、天蓝变豆菜提取物30‑60份、益生菌冻干粉45‑80份,本发明抗疲劳效果好且使用方便。
Description
技术领域
本发明涉及一种益生菌保健品及其制备方法,属于保健品技术领域。
背景技术
疲劳又称疲乏,是主观上一种疲乏无力的不适,感觉疲劳不是特异症状,很多疾病都可引起疲劳,很少有患病后感觉浑身不是劲的情况,不同疾病引起不同程度的疲劳,有些疾病表现更明显有时可作为就诊的首发症状。现代中医认为,疲劳为一个病名,有其病因病机,是临床上常见病、多发病,是必须重视的新病种,归于亚健康范畴,涉及五脏六腑,主要以脾、肝、肾为主。可以通过中医辨证组方调治,以达到预防、治疗、控制的目的。疲劳为元气耗伤之虚证与心理变化(或不畅)双重因素所致。治疗上不仅要补虚扶正,也应祛疲安神。一方面,补虚固元,滋养机体,虚得补,正则旺,疲劳自除;另一方面,调畅心志,舒心畅意,神得安,心则宁,疲劳可消。临床应用于缓解体力疲劳的药物和补品很多,但这些品种毒副作用较大,而且不方便服用,或没有经过药理验证,迄今为止,人们正在寻找一种制作简单、服用方便的抗疲劳的保健品。
发明内容
发明目的:本发明的目的是提供一种效果明显的益生菌保健品及其制备方法。
技术方案:本发明的目的是通过如下的方案实现的:
一种益生菌保健品,由下列重量份的原料制成:半叶马尾藻提取物60-100份、羽脉野扇花提取物40-80份、天蓝变豆菜提取物30-60份、益生菌冻干粉45-80份。
所述的益生菌保健品,益生菌冻干粉由以下重量份的菌组成:短双歧杆菌10-20份、德氏乳杆菌15-20份、干酪乳杆菌20-40份。
益生菌保健品,半叶马尾藻提取物的制备方法包括以下步骤:将半叶马尾藻粉碎至40-60目,取100g牛膝粉末,加入半叶马尾藻质量5-8倍、质量浓度为75%-90%的乙醇,再加入乙醇与半叶马尾藻总重量0.5-3.3%的琥珀辛酯磺酸钠,得混合物;将混合物在超声波频率为40KHz、超声波功率为100-200W、温度为30-50℃的条件下超声浸泡20-40min,然后在微波反应器中用微波加热20-30分钟,微波功率为260-520W,频率为2450MHz,静置、冷却,得超声提取液,蒸去溶剂,干燥,得到半叶马尾藻提取物。
所述的益生菌保健品,羽脉野扇花提取物的制备方法包括以下步骤:取中药材羽脉野扇花,剪短至1-2cm,加入8-10倍量的80%乙醇浸泡过夜,加热回流提取2-3次,每次2h,合并提取液,过滤,滤液减压回收至无醇味,得醇提物;在醇提物中加入1-1.5倍体积的沸点60℃-90℃的石油醚,液液萃取2-3次,分离除去石油醚部位;在石油醚萃取后的剩余液体中加入1-1.5倍体积的乙酸乙酯,液液萃取2-3次,分离除去乙酸乙酯部位;在乙酸乙酯萃取后的剩余液体中加入1-1.5倍体积的正丁醇,液液萃取2-3次,收集、合并萃取液,回收溶剂,浓缩至浸膏,60℃真空干燥,粉碎得羽脉野扇花提取物。
所述的益生菌保健品,天蓝变豆菜提取物的制备方法包括以下步骤:向天蓝变豆菜原料中加入8-12倍量的90-98%乙醇,50-80℃搅拌提取0.5-1.5h后滤出,得到第一提取液和第一滤渣,第一提取液备用;向第一滤渣中加入8-12倍量的90-98%乙醇,50-80℃搅拌提取0.5-1.5h后滤出,得到第二提取液和第二滤渣,第二提取液备用;向第二滤渣中加入8-12倍量的90-98%乙醇,50-80℃搅拌提取0.5-1.5h后滤出,得到第三提取液;合并第一提取液、第二提取液和第三提取液得初提液;浓缩至浸膏,60℃真空干燥,得天蓝变豆菜提取物。
所述的益生菌保健品,制备方法包括以下步骤:按重量份计,培养复合益生菌,冻干,按配方比例称取其他三种原料,混合,辐照灭菌。
所述的益生菌保健品,其特征在于,辐照灭菌条件:调控辐照剂量60rad,辐照剂量率240rad/min,辐照时间46h。
所述的益生菌保健品在制备抗疲劳保健品中的应用。
半叶马尾藻Sargassum hemiphyllum(Tuen.)C.Ag.产东海至南海。全草:软坚散结,消炎,利水,泄热。用于瘰疬,瘿瘤,积聚,水肿,脚气,睾丸肿痛,慢性气管炎,疝气。羽脉野扇花Sarcococca hookeriana Baill.产四川,西藏。全株:散瘀止血,行气止痛,拔毒生肌。用于胃痛,支气管炎,肝炎,蛔虫。外用治跌打损伤,刀伤出血,无名肿毒,黄水疮。天蓝变豆菜Sanicula coerulescens Franch.产湖北,四川,云南。全草:祛风散寒,清肺,化痰止咳,行血通经。用于风寒感冒,咳嗽,哮喘,***,经闭,腰痛。。
有益效果:
本发明益生菌保健品制备方法简单、配方合理、成本低廉,食用安全,无任何不良副作用,用于抗疲劳有明显效果。
具体实施方式
以下通过实施例形式,对本发明的上述内容再作进一步的详细说明,但不应将此理解为本发明上述主题的范围仅限于以下的实例,凡基于本发明上述内容所实现的技术均属于本发明的范围。
实施例1:取半叶马尾藻提取物80g、羽脉野扇花提取物60g、天蓝变豆菜提取物50g、益生菌冻干粉60g,益生菌冻干粉由以下重量的菌组成:短双歧杆菌15g、德氏乳杆菌15g、干酪乳杆菌30g。制备方法包括以下步骤:按重量份计,培养复合益生菌,冻干,按配方比例称取其他三种原料,混合,辐照灭菌。所述的益生菌保健品,其特征在于,辐照灭菌条件:调控辐照剂量60rad,辐照剂量率240rad/min,辐照时间46h。
半叶马尾藻提取物的制备方法包括以下步骤:将半叶马尾藻粉碎至40-60目,取100g牛膝粉末,加入半叶马尾藻质量5-8倍、质量浓度为75%-90%的乙醇,再加入乙醇与半叶马尾藻总重量0.5-3.3%的琥珀辛酯磺酸钠,得混合物;将混合物在超声波频率为40KHz、超声波功率为100-200W、温度为30-50℃的条件下超声浸泡20-40min,然后在微波反应器中用微波加热20-30分钟,微波功率为260-520W,频率为2450MHz,静置、冷却,得超声提取液,蒸去溶剂,干燥,得到半叶马尾藻提取物。
所述的益生菌保健品,羽脉野扇花提取物的制备方法包括以下步骤:取中药材羽脉野扇花100g,剪短至1-2cm,加入8-10倍量的80%乙醇浸泡过夜,加热回流提取2-3次,每次2h,合并提取液,过滤,滤液减压回收至无醇味,得醇提物;在醇提物中加入1-1.5倍体积的沸点60℃-90℃的石油醚,液液萃取2-3次,分离除去石油醚部位;在石油醚萃取后的剩余液体中加入1-1.5倍体积的乙酸乙酯,液液萃取2-3次,分离除去乙酸乙酯部位;在乙酸乙酯萃取后的剩余液体中加入1-1.5倍体积的正丁醇,液液萃取2-3次,收集、合并萃取液,回收溶剂,浓缩至浸膏,60℃真空干燥,粉碎得羽脉野扇花提取物。
所述的益生菌保健品,天蓝变豆菜提取物的制备方法包括以下步骤:向天蓝变豆菜100g原料中加入8-12倍量的90-98%乙醇,50-80℃搅拌提取0.5-1.5h后滤出,得到第一提取液和第一滤渣,第一提取液备用;向第一滤渣中加入8-12倍量的90-98%乙醇,50-80℃搅拌提取0.5-1.5h后滤出,得到第二提取液和第二滤渣,第二提取液备用;向第二滤渣中加入8-12倍量的90-98%乙醇,50-80℃搅拌提取0.5-1.5h后滤出,得到第三提取液;合并第一提取液、第二提取液和第三提取液得初提液;浓缩至浸膏,60℃真空干燥,得天蓝变豆菜提取物。
实施例2:抗疲劳的药效学研究
实验动物:昆明种小鼠,雄性,体重18-22g,上海斯莱克实验动物有限公司提供,生产许可证:SCXK(沪)2007-0005。
实验药物:按实施例1的制备方法制备样品,则按小鼠体重计,低剂量、中剂量和高剂量分别为20g生药量/kg、40g生药量/kg和80g生药量/kg。
阳性药精方堂牌养和胶囊,上海精方堂生物科技有限公司,国食健字G20050641,批号:20110321。规格:每粒装0.5g。病人使用一般每次2粒,每日2次,即人日用量2g,相当于每只20g小鼠用量为2*0.0026=0.0052g,按每公斤小鼠算,即阳性药组给予精方堂牌养和胶囊0.26g/kg。
实验过程:选健康小鼠50只,随机分出10只作为正常组,其余40只小鼠,随机分成4组:高剂量组、中剂量组、低剂量组、阳性药精方堂牌养和胶囊组,其中3个剂量组、精方堂牌养和胶囊组按上述剂量灌胃给药,正常组给0.2ml的水,每天上午1次,连续30d,进行小鼠负重游泳试验,小鼠尾部负小鼠体重5%的铅皮,放入水温为25℃,水深35cm的游泳箱中,用秒表记录自游泳至力竭死亡的时间,各组数据以均值±标准差表示,组间进行t测验。结果见表1
表1.本发明对小鼠负重游泳试验的影响(n=10)
注:同正常组相比*p<0.05**<0.01
结论:本发明与正常组比较,明显延长小鼠负重游泳时间,说明本发明具有抗疲劳作用,与阳性药比较,时间延长,说明本发明抗疲劳效果优于阳性药。
Claims (8)
1.一种益生菌保健品,其特征在于,由下列重量份的原料制成:半叶马尾藻提取物60-100份、羽脉野扇花提取物40-80份、天蓝变豆菜提取物30-60份、益生菌冻干粉45-80份。
2.如权利要求1所述的益生菌保健品,其特征在于,益生菌冻干粉由以下重量份的菌组成:短双歧杆菌10-20份、德氏乳杆菌15-20份、干酪乳杆菌20-40份。
3.如权利要求1所述的益生菌保健品,其特征在于,半叶马尾藻提取物的制备方法包括以下步骤:将半叶马尾藻粉碎至40-60目,取100g牛膝粉末,加入半叶马尾藻质量5-8倍、质量浓度为75%-90%的乙醇,再加入乙醇与半叶马尾藻总重量0.5-3.3%的琥珀辛酯磺酸钠,得混合物;将混合物在超声波频率为40KHz、超声波功率为100-200W、温度为30-50℃的条件下超声浸泡20-40min,然后在微波反应器中用微波加热20-30分钟,微波功率为260-520W,频率为2450MHz,静置、冷却,得超声提取液,蒸去溶剂,干燥,得到半叶马尾藻提取物。
4.如权利要求1所述的益生菌保健品,其特征在于,羽脉野扇花提取物的制备方法包括以下步骤:取中药材羽脉野扇花,剪短至1-2cm,加入8-10倍量的80%乙醇浸泡过夜,加热回流提取2-3次,每次2h,合并提取液,过滤,滤液减压回收至无醇味,得醇提物;在醇提物中加入1-1.5倍体积的沸点60℃-90℃的石油醚,液液萃取2-3次,分离除去石油醚部位;在石油醚萃取后的剩余液体中加入1-1.5倍体积的乙酸乙酯,液液萃取2-3次,分离除去乙酸乙酯部位;在乙酸乙酯萃取后的剩余液体中加入1-1.5倍体积的正丁醇,液液萃取2-3次,收集、合并萃取液,回收溶剂,浓缩至浸膏,60℃真空干燥,粉碎得羽脉野扇花提取物。
5.如权利要求1所述的益生菌保健品,其特征在于,天蓝变豆菜提取物的制备方法包括以下步骤:向天蓝变豆菜原料中加入8-12倍量的90-98%乙醇,50-80℃搅拌提取0.5-1.5h后滤出,得到第一提取液和第一滤渣,第一提取液备用;向第一滤渣中加入8-12倍量的90-98%乙醇,50-80℃搅拌提取0.5-1.5h后滤出,得到第二提取液和第二滤渣,第二提取液备用;向第二滤渣中加入8-12倍量的90-98%乙醇,50-80℃搅拌提取0.5-1.5h后滤出,得到第三提取液;合并第一提取液、第二提取液和第三提取液得初提液;浓缩至浸膏,60℃真空干燥,得天蓝变豆菜提取物。
6.如权利要求1所述的益生菌保健品,其特征在于,制备方法包括以下步骤:按重量份计,培养复合益生菌,冻干,按配方比例称取其他三种原料,混合,辐照灭菌。
7.如权利要求5所述的益生菌保健品,其特征在于,辐照灭菌条件:调控辐照剂量60rad,辐照剂量率240rad/min,辐照时间46h。
8.如权利要求1所述的益生菌保健品在抗疲劳保健品中的应用。
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