CN106509421A - Microecological preparation culture medium for cattle prepared from guar meal and cassava residues - Google Patents
Microecological preparation culture medium for cattle prepared from guar meal and cassava residues Download PDFInfo
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- CN106509421A CN106509421A CN201611190367.XA CN201611190367A CN106509421A CN 106509421 A CN106509421 A CN 106509421A CN 201611190367 A CN201611190367 A CN 201611190367A CN 106509421 A CN106509421 A CN 106509421A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention relates to the technical field of feed additives and more particularly relates to a microecological preparation fermentation medium for cattle prepared from guar meal and cassava residues as base materials. The medium is a solid medium. The medium comprises the following raw materials in parts by mass: 100 parts of guar meal, 100 parts of cassava residues, 18-23 parts of bran, 8-10 parts of glucose, 7-10 parts of soybean meal, 3-5 parts of urea, 3-4 parts of ammonium sulfate, 0.2-0.5 part of potassium dihydrogen phosphate, and 0.3-0.4 part of magnesium sulfate. The provided microecological preparations aim at the development of cattle breeding, are strong in targets, and can relatively obviously improve the disease resistance of the cattle. Besides, the preparation technology is relatively mature, the energy consumption is relatively low, the production process is clean and free of pollution, the production cost is cheap, and the technological effect is obvious, so that the medium has a relatively good practical value and a promotion and application value.
Description
Technical field
Field of feed additive technology of the present invention, and in particular to a kind of is prepared by base material using guar meal, manioc waste
Cattle microbial ecological agent fermentation medium.
Background technology
Since the middle of last century finds that adding antibiotic in feedstuff has growth-promoting effect, antibiotic is extensive
Be applied to animal husbandry, and achieve larger economic benefit in husbandry sector.But antibiotic is used in a large number for a long time so that
Pathogenic microorganism produces drug resistance, livestock products drug residue and poultry body autoimmunity and becomes increasingly conspicuous the problems such as decline,
The generation that causes livestock and poultry is increased, mortality of livestock increasingly rises, and brings huge challenge to husbandry sector.Simultaneously
In animal body, the antibiotic etc of residual also has potentially hazardous property by the accumulation of food chain to human health.Therefore,
Countries in the world start to take the use of antibiotic and the measure such as limit the use of and disable, while actively finding new green, safe efficient
Antibiotic substitute.
Microbial ecological agent is a kind of green, safe and efficient feed additive, has no toxic side effect, has no drug resistance, can adjust
Microecological balance in whole and maintenance animal gastrointestinal tract, enhancing human body immunity promote digesting and assimilating for nutrition.While its tunning
In contain substantial amounts of vitamin, trace element, aminoacid and some unknown growth promoting substances, farming animals can be improved and increased day by day
Weight, meat productss quality.
At present, in existing feed additive, microbial ecological agent used has lactic acid bacteria class (lactobacilluss, bacillus bifiduss etc.), bud
The antibacterial classes such as spore bacillus class (bacillus subtilises, Bacillus licheniformis etc.), aspergillosis class (aspergillus niger, aspergillus oryzae) and yeast class
Single bacteria preparation and composite bacteria preparations such as (beer yeast and saccharomyces cerevisiaes).But Preliminary Applications effect shows, these Tiny ecosystem systems
When agent is applied to the ruminants such as finished cattle, due to for feedstuff lack of targeted, thus resulting in and can not effectively improve in feedstuff
Therefore utilization of nutrients, also have impact on popularization and application of the microbial ecological agent in cattle feedstuff.
On the other hand, with the development of food industry deep processing, for example, in bean cake processing industry, Maninot esculenta crantz. processing industry, can often produce
The biological wastes such as the substantial amounts of guar meal of life, manioc waste, and these waste materials are typically only capable to fermentation due to lacking Processes For Effective Conversion
Bury discarded afterwards as farmland base fertilizer or directly, cause the production cost that increased enterprise to a certain extent.Due to Guar
Containing nutritional labelings such as abundant carbohydrate, vitamin, aminoacid and mineral in the biomaterials such as bean cake, manioc waste,
Carbon-nitrogen ratio is moderate, is the preferable basic material of fermentable after proper treatment, thus to these biomaterials if in addition
Make full use of, reducing food processing enterprises waste processing cost simultaneously, also preferably reduce the production of biofermentation product into
This, with preferable economic worth and practical value.
The content of the invention
Present invention aim at providing a kind of sending out as base material with guar meal, manioc waste for cattle microbial ecological agent fermentation
Ferment culture medium, with reference to the culture medium, coordinates the optimum organization of fermentable bacterial strain, can preparative preparation cattle Tiny ecosystem system
Agent, so as to improve the nutrient utilization of cattle feedstuff, while reduce the application of corresponding antibiotic.
Details are as follows for the technical solution used in the present invention.
Cattle microbial ecological agent culture medium prepared by a kind of guar meal, manioc waste, is solid medium, with mass parts
Number meter, culture medium raw material have：100 parts of guar meal after drying（Air-dry or dry, moisture content is 8 ~ 14% after being dried）, after drying
100 parts of manioc waste（Air-dry or dry, moisture content is 8 ~ 14% after being dried）, wheat bran 18-23 parts, 8～10 parts of glucose, bean cake
7～10 parts, 3～5 parts of carbamide, 3～4 parts of ammonium sulfate, 0.2～0.5 part of potassium dihydrogen phosphate, 0.3～0.4 part of magnesium sulfate.
Cattle microbial ecological agent culture medium prepared by the guar meal, manioc waste, more excellent proportioning is：Guar meal
100 parts, 100 parts of manioc waste, 20 parts of wheat bran, 8 parts of glucose, 8 parts of bean cake, 4 parts of carbamide, 3 parts of ammonium sulfate, biphosphate
0.3 part of potassium, 0.3 part of magnesium sulfate.
Using the cattle cattle of microbial ecological agent culture medium microbial ecological agent preparation method, following step is specifically included
Suddenly：
（1）Prepare seed liquor,
Ruminococcus albus, bacillus acidophilus, Bacillus subtillis, yeast difference inoculation fermentation are prepared into seed liquor；
In the seed liquor of preparation, for guaranteeing to be inoculated with after fermentation effect, the bacterium number specific requirement of each strain is：Ruminococcus albus are not
Less than 1 × 109Cfu/L, bacillus acidophilus are not less than 4 × 109 Cfu/L, bacillus subtilises are not less than 7 × 109 cfu/
The inferior Dbaly yeast bacterium of L, the Chinese is not less than 2 × 108cfu/L；
During fermentation, Ruminococcus albus, bacillus acidophilus, Bacillus subtillis, yeast can be seeded to respectively LB fluid mediums,
In MRS fluid mediums, meat soup fluid medium, PDA liquid medium, cultivate 17～25 hours at 30 DEG C～37 DEG C；
The Ruminococcus albus, can voluntarily screen acquisition；
The bacillus acidophilus, the concrete preserving number for being, for example, Chinese microorganism strain preservation administrative center is for CICC20985's
Bacterial strain；
The Bacillus subtillis, the preserving number of concrete for example, Chinese microorganism strain preservation administrative center is CICC10732
Bacterial strain；
The yeast, concrete is, for example, the Han Xunde of the preserving number for CICC31235 of Chinese microorganism strain preservation administrative center
Bali yeast strain；
（2）Solid fermentation culture medium is prepared,
Solid fermentation culture medium each raw material was crushed into 40 mesh sieves respectively, 100～130 parts of distilled water is added after mix homogeneously, is stirred
Mix uniform, 121 DEG C of 20 ~ 30min of sterilizing are cooled to room temperature standby；
（3）Solid fermentation,
By step（1）Middle seed liquor is mixed, with volume basis, Ruminococcus albus seed liquor：Bacillus acidophilus' seed liquor：
The inferior Dbaly yeast seed liquor of the Chinese：Bacillus subtillis seed liquor=2～3：2～4：1～2：2～4, by the mixing of mix homogeneously
Seed liquor is with 6 ~ 12% ratio（V/W, mL/g）It is inoculated into step（2）Solid fermentation culture medium on；
After inoculation, 30 ~ 38 DEG C of condition 48 ~ 96h of bottom fermentation（Regular turnover during gravity-flow ventilation, fermentation）, solid fermentation is obtained and produces
Thing；
After fermentation ends, by obtained solid fermentation product under the conditions of 50 ~ 60 DEG C cold drying to moisture content≤10%,
Crush, cattle microbial ecological agent when as feeding；
Prepared cattle microbial ecological agent, for guaranteeing preferable using effect, concrete viable count requirement is：
Ruminococcus albus >=2 × 108CFU/g,
Bacillus acidophilus >=3 × 108CFU/g,
Inferior Dbaly yeast bacterium >=1 × 10 of the Chinese8CFU/g,
Bacillus subtilises >=2 × 108CFU/g。
Ruminococcus albus（Ruminococcusalbus）, it is distinctive strain in a kind of bovine rumen, the bacterium can secrete
The enzyme of specific degradation cellulose.The application by by this strain and the species specific matched combined of other bacterium, for specific
Property microecosystem regulation formulation products to cattle feed improvement result, so as to increase the using effect of microbial ecological agent.In general,
The major technique novelty of the application is embodied in following aspects：
1st, Ruminococcus albus targetedly, are innovatively added in microbial ecological agent, in prepared microbial ecological agent,
Strain eurythmy, growth conditions are excellent, and with unique fruit aroma, palatability is strong, and the body that preferably improve cattle is exempted from
The utilization rate of epidemic disease power and feedstuff, while the problems such as can reducing cattle manure foul odour just and then mitigate breeding pollution；
When the 2nd, preparing microbial ecological agent, the Major Nutrient material with biomass waste materials such as guar meal, manioc wastes as culture medium, because
And production cost is relatively low, while also preferably solving the difficulty that a large amount of biomass waste materials are difficult to effective process and easy environmental pollution
Topic；
3rd, the microbial ecological agent prepared by the application, which is directed to cattle feeding research and development, with strong points, more can significantly improve cattle
Premunition, adds that its preparation technology is more ripe, and energy consumption is relatively low, production process cleanliness without any pollution, adds low production cost, skill
Art effect is significant, thus there is preferably practical value and application value.
Specific embodiment
Explanation is further explained to the application with reference to embodiment, before further explaining, with regard to following realities
It is related to the briefly introduction of part biological material context to be described as follows in applying example.
Microbial strains：
Bacillus acidophilus used in following embodiments, from Chinese microorganism strain preservation administrative center, preserving number is
CICC20985；
Bacillus subtillis used, from Chinese microorganism strain preservation administrative center, preserving number is CICC10732；
Used yeast, from Chinese microorganism strain preservation administrative center, Han Xunde Bali ferment of the preserving number for CICC31235
It is female；
Ruminococcus albus used in following embodiments, by inventor using sodium carboxymethyl cellulose plating medium from bovine rumen
Middle screening is obtained（The bacterial strain belongs to a kind of conventional bacterial strain, it is easy to which screening is obtained）, the sodium carboxymethyl cellulose plating medium
（Ruminococcus albus screening culture medium）, per the formula of 1000mL it is：20g sodium carboxymethyl cellulose（CMC-Na）、 2g
KH2PO4、1.4g(NH4)2SO4 、0.3g MgSO4、0.3g CaCl2, 1g cysteine, 20g agar powders；
Need to explain and illustrate, above-mentioned bacterial strains are and can openly obtain, or voluntarily screen the conventional bacterial strain of acquisition, the present invention
Technical scheme not necessarily depend on above-mentioned specific bacterial strain, similar technique effect can also be obtained using similar strain；
Fermentation culture sill：
Manioc waste, from Sanmenxia City Jun Jian Food Co., Ltd, using front detection：Crude fibre 11.9%, coarse ash 2.1%,
Moisture 11.8%；
Guar meal, from Nanning natural resources planting base, using front detection：Crude protein 48.58%, coarse ash
12.36%th, moisture 8.86%.
Embodiment 1
Cattle microbial ecological agent culture medium prepared by guar meal that the present embodiment is provided, manioc waste, is solid medium,
In terms of mass fraction, culture medium raw material has：100 parts of guar meal, 100 parts of manioc waste, 20 parts of wheat bran, 8 parts of glucose, bean
8 parts of the dregs of rice, 4 parts of carbamide, 3 parts of ammonium sulfate, 0.3 part of potassium dihydrogen phosphate, 0.3 part of magnesium sulfate.
Using the cattle microbial ecological agent prepared by the culture medium, it is made by the steps and forms：
（1）Prepare seed liquor,
Ruminococcus albus, bacillus acidophilus, Bacillus subtillis, the inferior Dbaly yeast of the Chinese are seeded to into LB liquid cultures respectively
In base, MRS fluid mediums, meat soup fluid medium, PDA liquid medium, cultivate 24 hours at 37 DEG C；
In the seed liquor of preparation, Ruminococcus albus content is 1.2 × 109Cfu/L, bacillus acidophilus' content are 4.5 × 109
Cfu/L, bacillus subtilis bacterial content are 7.6 × 109The inferior Dbaly yeast bacterial content of cfu/L, the Chinese is 2.8 × 108 cfu/L。
（2）Solid fermentation culture medium is prepared,
Solid fermentation culture medium each raw material was crushed into 40 mesh sieves respectively, 110 parts of distilled water after mix homogeneously, was added, stirring was equal
Even, 121 DEG C of sterilizing 20min are cooled to room temperature standby.
（3）Solid fermentation,
By step（1）Middle seed liquor is mixed, with volume basis, Ruminococcus albus seed liquor：Bacillus acidophilus' seed liquor：
The inferior Dbaly yeast seed liquor of the Chinese：Bacillus subtillis seed liquor=3：3：1：3, by the seed mixture liquid of mix homogeneously with 10%
Ratio（V/W, mL/g）It is inoculated into step（2）Solid fermentation culture medium on；
After inoculation, 35 DEG C of condition bottom fermentation 45h（Gravity-flow ventilation, uniformly stir once every 6h）, solid fermentation product is obtained；
After fermentation ends, by obtained solid fermentation product, under the conditions of 50 DEG C, cold drying to moisture content is 6.5% or so
Afterwards, crush, cattle microbial ecological agent when as feeding；
Prepared cattle microbial ecological agent, concrete viable count is：
Ruminococcus albus（3~4）×108CFU/g,
Bacillus acidophilus（5~6）×108CFU/g,
The inferior Dbaly yeast bacterium of the Chinese（1.0~2.0）×108CFU/g,
Bacillus subtilises（2.0~3.0）×108CFU/g。
Experimental example：
To the microbial ecological agent prepared by the present embodiment, inventor has carried out feeding experiment in being further added into cattle feedstuff,
Related experiment process is briefly discussed below.
Experimental animal is selected and packet design：
Randomly choose 12 monthly ages health, physically well develop, average weight is that the western miscellaneous of 200kg or so castrates bull 150, test point
For 5 groups, per group of 3 repetitions, each repeats 10 cattle, and between each group, in group, whose body weight difference is not notable（P ＞ 0.05）；
Matched group, feeds basal diet；
Test group（SYA）, in basal diet, add the present embodiment microbial ecological agent（Addition mass ratio 1%）；
Test group（SYB）, in basal diet, add the present embodiment microbial ecological agent（Adding proportion 2%）；
Test group（SYC）, in basal diet, add the present embodiment microbial ecological agent（Adding proportion 4%）；
Experimental group (SYD), adds chlortetracycline in basal diet（Adding proportion is 100ppm）.
Basal diet reference《China Feeding Standard of Beef Cattle》（2005）It is designed, Ju Ti Pei Fang is as follows：
Note：Compound premix provides ferrum 52mg, 35 mg of zinc, 10 mg of copper, 22 mg of manganese, 0.12 mg of cobalt, selenium for every kilogram of daily ration
0.12 mg, vitamin A 9120IU, vitamin D 2200IU, Vitamin E 18IU.
Experimentation：
In open cowshed, foster feeding is fastened on single hurdle for cattle breeding, is fed twice, respectively at the daily morning 06: 30 and afternoon 5 day:
30 feedings；During test, cattle free water, colony house keep hygienically clean, and other programs are routinely carried out according to test site；
Test in 1 day April in 2015,1 end of day of July in 2015, altogether 90 d.
Experimental result：
In experimentation, test ox body weight is weighed in on-test on an empty stomach with the end of respectively, and calculate average daily gain
（ADG）；
Accurate Determining and record the daily feedstuff of every cattle and feed amount during test, and calculate experimental period average dry daily ingestion amount
（ADMI）；
Feedstuff-meat ratio is calculated according to ADG and ADMI（ADMI/ADG）.
After feeding experiment terminates, related data is counted, microbial ecological agent and chlortetracycline are to finished cattle growth performance
Impact result it is as shown in the table：
。
From the point of view of above-mentioned result of the test, addition originally apply new microbial ecological agent in example can significantly improve finished cattle daily gain,
Efficiency of feed utilization, reduction feedstuff-meat ratio, with greater advantage compared with matched group.Its with addition chlortetracycline group compared with, daily gain, feeding
Material utilization rate, feed-weight ratio effect is essentially identical, but this new microbial ecological agent it is safer, it is green avoid antibiotic remainss and
Drug resistance problems.On the whole, the new microbial ecological agent is relative to matched group, chlortetracycline group, can be with greater advantage
Large-scale application in actual cattle farm.
Claims (6)
1. the cattle microbial ecological agent culture medium that prepared by a kind of guar meal, manioc waste, it is characterised in that the culture medium is solid
Body culture medium, in terms of mass fraction, culture medium raw material proportioning is：100 parts of guar meal, 100 parts of manioc waste, wheat bran 18 ~ 23
Part, 8～10 parts of glucose, 7～10 parts of bean cake, 3～5 parts of carbamide, 3～4 parts of ammonium sulfate, potassium dihydrogen phosphate 0.2～0.5
Part, 0.3～0.4 part of magnesium sulfate.
2. the cattle microbial ecological agent culture medium that prepared by guar meal as claimed in claim 1, manioc waste, it is characterised in that
Culture medium raw material proportioning is：100 parts of guar meal, 100 parts of manioc waste, 20 parts of wheat bran, 8 parts of glucose, 8 parts of bean cake, urine
4 parts of element, 3 parts of ammonium sulfate, 0.3 part of potassium dihydrogen phosphate, 0.3 part of magnesium sulfate.
3. the cattle of the microbial ecological agent culture medium microbial ecological agent preparation method of cattle described in claim 1 or 2 is utilized, and which is special
Levy and be, specifically include following steps：
（1）Prepare seed liquor,
Ruminococcus albus, bacillus acidophilus, Bacillus subtillis, yeast difference inoculation fermentation are prepared into seed liquor；
In seed liquor, the bacterium number specific requirement of each strain is：Ruminococcus albus are no less than 1 × 109Cfu/L, bacillus acidophilus
Not less than 4 × 109 Cfu/L, bacillus subtilises are not less than 7 × 109Cfu/L, yeast are not less than 2 × 108cfu/L；
（2）Solid fermentation culture medium is prepared,
Culture medium each raw material was crushed into 40 mesh sieves respectively, 100～130 parts of distilled water is added after mix homogeneously, is stirred,
121 DEG C of 20 ~ 30min of sterilizing, are cooled to room temperature standby；
（3）Solid fermentation,
By step（1）Middle seed liquor is mixed, with volume basis, Ruminococcus albus seed liquor：Bacillus acidophilus' seed liquor：
The inferior Dbaly yeast seed liquor of the Chinese：Bacillus subtillis seed liquor=2～3：2～4：1～2：2～4, by the mixing of mix homogeneously
Seed liquor is inoculated into step（2）Solid fermentation culture medium on；
After inoculation, 30 ~ 38 DEG C of condition 48 ~ 96h of bottom fermentation are obtained solid fermentation product；
After fermentation ends, obtained solid fermentation product is dried to moisture content≤10% under the conditions of 50 ~ 60 DEG C, is crushed,
Cattle microbial ecological agent when as feeding.
4. cattle as claimed in claim 3 microbial ecological agent preparation method, it is characterised in that step（1）In, prepare seed liquor
When, Ruminococcus albus, bacillus acidophilus, Bacillus subtillis, yeast can be seeded to LB fluid mediums, MRS liquid respectively
Culture medium, meat soup fluid medium, PDA liquid medium.
5. cattle as claimed in claim 3 microbial ecological agent preparation method, it is characterised in that step（1）In,
The bacillus acidophilus, are the bacterial strain of the preserving number for CICC20985 of Chinese microorganism strain preservation administrative center；
The Bacillus subtillis, are the bacterial strain of the preserving number for CICC10732 of Chinese microorganism strain preservation administrative center；
The yeast, is the Chinese inferior Dbaly yeast of the preserving number for CICC31235 of Chinese microorganism strain preservation administrative center
Bacterial strain.
6. cattle as claimed in claim 3 microbial ecological agent preparation method, it is characterised in that step（3）In, cattle Tiny ecosystem system
In agent, concrete viable count requirement is：
Ruminococcus albus >=2 × 108CFU/g,
Bacillus acidophilus >=3 × 108CFU/g,
Yeast >=1 × 108CFU/g,
Bacillus subtilises >=2 × 108CFU/g。
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Citations (6)
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| CN101138392A (en) * | 2007-07-30 | 2008-03-12 | 湖南农业大学 | Micro-zoology preparations for feeding |
| CN102948612A (en) * | 2012-12-18 | 2013-03-06 | 陈建军 | Fodder solid fermenting method |
| CN103652328A (en) * | 2013-12-13 | 2014-03-26 | 广西科技大学 | Method for producing high-quality high-protein feed through mixed strain solid-state fermentation of rice dregs and manioc waste |
| CN103960479A (en) * | 2014-05-30 | 2014-08-06 | 青岛科奈尔饲料有限公司 | High-protein orange pomace fermented feed and preparation technique thereof |
| CN104431415A (en) * | 2014-10-28 | 2015-03-25 | 亿利资源集团有限公司 | Active microorganism feed for ruminant animals |
| CN105010758A (en) * | 2015-06-30 | 2015-11-04 | 河南双成生物科技有限公司 | Method of producing protein feed by liquid-solid two-step fermentation method |
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2016
- 2016-12-21 CN CN201611190367.XA patent/CN106509421A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138392A (en) * | 2007-07-30 | 2008-03-12 | 湖南农业大学 | Micro-zoology preparations for feeding |
| CN102948612A (en) * | 2012-12-18 | 2013-03-06 | 陈建军 | Fodder solid fermenting method |
| CN103652328A (en) * | 2013-12-13 | 2014-03-26 | 广西科技大学 | Method for producing high-quality high-protein feed through mixed strain solid-state fermentation of rice dregs and manioc waste |
| CN103960479A (en) * | 2014-05-30 | 2014-08-06 | 青岛科奈尔饲料有限公司 | High-protein orange pomace fermented feed and preparation technique thereof |
| CN104431415A (en) * | 2014-10-28 | 2015-03-25 | 亿利资源集团有限公司 | Active microorganism feed for ruminant animals |
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