CN106508824A - Pretreatment method for efficiently improving ova hatching rate in good year - Google Patents
Pretreatment method for efficiently improving ova hatching rate in good year Download PDFInfo
- Publication number
- CN106508824A CN106508824A CN201611161147.4A CN201611161147A CN106508824A CN 106508824 A CN106508824 A CN 106508824A CN 201611161147 A CN201611161147 A CN 201611161147A CN 106508824 A CN106508824 A CN 106508824A
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- Prior art keywords
- ovum
- shelling
- hatching
- worm
- water
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000012447 hatching Effects 0.000 title claims abstract description 18
- 238000002203 pretreatment Methods 0.000 title abstract 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 42
- 238000003756 stirring Methods 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000004061 bleaching Methods 0.000 claims abstract description 16
- 239000013505 freshwater Substances 0.000 claims abstract description 16
- 239000010813 municipal solid waste Substances 0.000 claims abstract description 14
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 210000004681 ovum Anatomy 0.000 claims description 55
- 102000002322 Egg Proteins Human genes 0.000 claims description 54
- 108010000912 Egg Proteins Proteins 0.000 claims description 54
- 235000013601 eggs Nutrition 0.000 claims description 23
- 241000238426 Anostraca Species 0.000 claims description 19
- 239000000243 solution Substances 0.000 claims description 18
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 15
- 238000011534 incubation Methods 0.000 claims description 14
- 125000001309 chloro group Chemical group Cl* 0.000 claims description 12
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 8
- 238000012544 monitoring process Methods 0.000 claims description 7
- 238000002791 soaking Methods 0.000 claims description 6
- 235000015655 Crocus sativus Nutrition 0.000 claims description 3
- 244000124209 Crocus sativus Species 0.000 claims description 3
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 239000002574 poison Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 238000011897 real-time detection Methods 0.000 claims description 3
- 230000000630 rising effect Effects 0.000 claims description 3
- 239000011435 rock Substances 0.000 claims description 3
- 235000013974 saffron Nutrition 0.000 claims description 3
- 239000004248 saffron Substances 0.000 claims description 3
- 239000003643 water by type Substances 0.000 claims description 3
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 238000009360 aquaculture Methods 0.000 abstract description 4
- 241000133262 Nauplius Species 0.000 abstract description 3
- 244000144974 aquaculture Species 0.000 abstract description 3
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 3
- 238000002156 mixing Methods 0.000 abstract description 2
- 239000011259 mixed solution Substances 0.000 abstract 2
- 230000001590 oxidative effect Effects 0.000 abstract 1
- 239000013535 sea water Substances 0.000 abstract 1
- 241000143060 Americamysis bahia Species 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 235000004237 Crocus Nutrition 0.000 description 1
- 241000596148 Crocus Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000595940 Notostraca Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000001136 chorion Anatomy 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a pretreatment method for efficiently improving ova hatching rate in a good year, and belongs to the technical field of aquaculture. The method includes steps of mixing bleaching water with a certain concentration and a certain quantity of tablet sodium hydroxide according to a certain ratio, and preparing to be a mixed solution; then stirring the mixed solution until the sodium hydroxide is completely dissolved, and then adding to the ova in good year after being soaked in fresh water; stirring while controlling temperature through adding trash ice; through this process, completely oxidizing and removing the outer shell of the ova in good year; after cleaning, adding to a seawater hatching pond to hatch; the ova in good year after being treated by the pretreatment method only has an embryo coated by a hatching film for being free from the binding of the outer shell, therefore, it is easier to hatch the nauplius; the hatching rate and the hatching speed are obviously improved.
Description
Technical field
The invention belongs to technical field of aquaculture, especially a kind of efficient pretreatment side for improving brine shrimp eggs incubation rate
Method.
Background technology
Shrimps, it is that one kind is distributed widely in global and halophilic small-sized shellfish.Brine shrimp ovum is by rich
Resting egg produced by year shrimp, the brine shrimp ovum recorded in the world have more than 100 strain.Shrimps nauplius has big
The ovum of amount, and containing rich in protein and fat (note:Wherein protein containing about 60%, fat containing about 20%), its adult institute
It is also very high containing nutrition.Therefore, shrimps are constantly subjected to the extensive attention of people as a kind of important biological feed.When
On former world, the nursery of more than 85% aquiculture animal is using shrimps as active bio feed source.And the current good year
The production technology of worm foolproof production model always for simple coarse, causes the incubation rate of worm's ovum all to exist always in the industry
50%-70% hovers, and incubation rate is relatively low in the general 24h incubation cycles, so as to draw high production cost.With aquaculture row
Increasingly the development of industry, breeding industry are more and more difficult, and nursery is into several low, of poor quality, the cost more and more higher of production, profit margin
It is less and less, the larger fairy shrimp bait plate of component is occupied in cost, if can be relatively low by cost control, be to nursery industry
One it is big contribute, but as the price level of this several years worm's ovums gradually rises, improve egg hatch rate become it is the most urgent
With an important tackling key problem difficult problem.
The content of the invention
It is an object of the invention to overcome the shortcomings of above-mentioned prior art, there is provided one kind can reduce production cost, improve
The preprocess method of brine shrimp eggs incubation rate.
The technical solution adopted for the present invention to solve the technical problems is:It is a kind of it is efficient improve brine shrimp eggs incubation rate it is pre-
Processing method, it is characterised in that:Comprise the steps:S1:Shelling barreled freshwater soaking brine shrimp eggs 1 first with conical bottom are little
When;S2:Add toward in shelling bucket again and use preprepared trash ice, and monitoring temperature, worm's ovum is cooled to into 21 degree;S3:Drop
After temperature, start to prepare dejacketing solution, specifically flaky sodium hydrate is added in bleaching water, and stirring at once is up to sheet
Till NaOH is completely dissolved;S4:The dejacketing solution for preparing is poured at once inside shelling bucket, with the worm's ovum after cooling
Mixing, and while at the uniform velocity stir;S5:While at the uniform velocity stirring, the solution temperature inside real-time detection shelling bucket, is being risen
When to 29 degree, it is necessary to plus trash ice cooling, and temperature will stablize the color that worm's ovum is observed in the range of 28-30 degree Celsius
Change, from canescence to brown to it is light yellow it is last to it is saffron when stop stirring, and at once by opening below shelling bucket
Close and open, and ovum net is received with 150 mesh collect worm's ovum;S6:After the worm's ovum inside shelling bucket has been collected with receipts ovum net, that is, use fresh water
Will be attached to the bleaching water above brine shrimp eggs to clean up;S7:After the shelling ovum for cleaning up is drained the water, amount is divided on demand
Egg hatching, during hatching 56 purpose of standby hatch net carry out hatching 24 hours.
Further, the process of ovum is steeped in step S1, specifically:Measure according to demand first, five are used according to one jin of ovum
The ratio of fresh water is risen soaking 1 hour, wherein, if the fresh water for using is running water, need first to detect whether with chlorine residue,
Can just use without chlorine residue.
Further, when adding trash ice in step S2, can be with by the way of stirring when trash ice is added, and in real time
Monitoring temperature, when treating that temperature reaches 21 degree, stops stirring and on the rocks.
Further, the compound method of the dejacketing solution in step S3 is:Required raw material be 10% bleaching water and
99% flaky sodium hydrate, according to Production requirement, is entered with the ratio of 1.5 liters of bleaching waters and 60 grams of NaOH according to 1 jin of worm's ovum
Row mixed preparing, needs to wear when preparation sour gas anti-poison respirator, and stirs flaky sodium hydrate to being completely dissolved
Till.
Further, after the ovum that shells in step S6 is accepted with the receipts ovum net of 150 mesh, need dry with fresh water flush
Only the bleaching water on shelling ovum surface is attached to, clean level is tested with chlorine residue kit, and chlorine residue is judged to cleaning when being 0mg/L
Totally.
The invention has the beneficial effects as follows:It is simple to operate, by using above-mentioned preprocess method, worm's ovum shell being oxidized away,
Do not have the constraint of shell, shelling ovum is the only coated embryo of a surplus hatching membrane, is more prone to hatch nauplius, incubation rate
All it is improved obviously with hatching speed.During practical application, egg hatch rate can bring up to 85%-90%.
Specific embodiment
With reference to embodiments the present invention is explained further, but embodiment does not do any type of limit to the present invention
It is fixed.
A kind of efficient preprocess method for improving brine shrimp eggs incubation rate of the present invention, comprises the steps:
S1:The shelling barreled freshwater soaking brine shrimp eggs of conical bottom are used first 1 hour.Shelling bucket is using the production of Zhanjiang Ming Xing companies
The brine shrimp eggs shelling bucket of volume 500L.
S2:Add toward in shelling bucket again and use preprepared trash ice, and monitoring temperature, worm's ovum is cooled to into 21 degree.
S3:After cooling, start to prepare dejacketing solution, specifically flaky sodium hydrate is added in bleaching water, and at once
Stirring is till the NaOH of sheet is completely dissolved.
S4:The dejacketing solution for preparing is poured into inside shelling bucket at once, is mixed with the worm's ovum after cooling, and it is while even
Speed stirring.
S5:While at the uniform velocity stirring, the solution temperature inside real-time detection shelling bucket, when rising to 29 degree,
It is accomplished by plus trash ice cooling, and temperature will be stablized in the range of 28-30 degree Celsius, observe the color change of worm's ovum, from greyish white
Color to brown to it is light yellow finally arrive saffron when stop stirring, and at once the switch below shelling bucket is opened, is used in combination
150 mesh are received ovum net and collect worm's ovum.
S6:After the worm's ovum inside shelling bucket has been collected with receipts ovum net, i.e., will be attached to above brine shrimp eggs with fresh water
Bleaching water is cleaned up.
S7:After the shelling ovum for cleaning up is drained the water, an amount point egg hatching on demand, during hatching, 56 purpose of standby is hatched net
Carry out hatching 24 hours.
The process of ovum is steeped in above-mentioned steps S1, specifically:Measure according to demand first, according to one jin of ovum ratio of five liters of fresh water
Example is soaking 1 hour, it is therefore an objective to which worm's ovum to be allowed fully absorbs water, and chorion produces tension force, more conducively hatches.Wherein, if used
Fresh water is running water, then need first to detect whether with chlorine residue, can just be used without chlorine residue.
When adding trash ice in above-mentioned steps S2, can be with by the way of stirring when trash ice is added, and real-time monitoring temperature,
When treating that temperature reaches 21 degree, stop stirring and on the rocks.The purpose of this process is to prevent follow-up dejacketing solution
Add the problem for worm's ovum embryo being burnt to death to be fiercely rapidly heated, so will lower the temperature in advance, this is conducive to the suitable of follow-up shelling reaction
Profit is carried out.
Further, the compound method of the dejacketing solution in step S3 is:Required raw material be 10% bleaching water and
99% flaky sodium hydrate, according to Production requirement, is entered with the ratio of 1.5 liters of bleaching waters and 60 grams of NaOH according to 1 jin of worm's ovum
Row mixed preparing, needs to wear when preparation sour gas anti-poison respirator, and stirs flaky sodium hydrate to being completely dissolved
Till.After described dejacketing solution has been prepared, a now with the current principle to be followed, the dejacketing solution for preparing is poured into shelling
Bucket the inside, is mixed with the worm's ovum after cooling, while at the uniform velocity stir, and the solution temperature inside real-time monitoring shelling bucket.
When rising to 29 degree, it is necessary to plus trash ice is lowered the temperature, and also temperature will be stablized at 29 degree or so, and critical maximum temperature is less than 30
Degree, observes the color change of worm's ovum, to light yellow last to stopping stirring when crocus from canescence to brown, and at once
Opening will be switched below shelling bucket, and ovum net is received with 150 mesh collect worm's ovum.
Further, after the ovum that shells in step S6 is accepted with the receipts ovum net of 150 mesh, need dry with fresh water flush
Only the bleaching water on shelling ovum surface is attached to, clean level is tested with chlorine residue kit, and chlorine residue is judged to cleaning when being 0mg/L
Totally.
Above content is only to illustrate technical scheme, technology of the one of ordinary skill in the art to the present invention
Simple modification or equivalent that scheme is carried out, all without departing from the spirit and scope of technical solution of the present invention.
Claims (5)
1. it is a kind of it is efficient improve brine shrimp eggs incubation rate preprocess method, it is characterised in that:Comprise the steps:
S1:The shelling barreled freshwater soaking brine shrimp eggs of conical bottom are used first 1 hour;
S2:Add toward in shelling bucket again and use preprepared trash ice, and monitoring temperature, worm's ovum is cooled to into 21 degree;
S3:After cooling, start to prepare dejacketing solution, specifically flaky sodium hydrate is added in bleaching water, and stir at once
Till the NaOH of sheet is completely dissolved;
S4:The dejacketing solution for preparing is poured into inside shelling bucket at once, is mixed with the worm's ovum after cooling, and while is at the uniform velocity stirred
Mix;
S5:While at the uniform velocity stirring, the solution temperature inside real-time detection shelling bucket, when rising to 29 degree, is just needed
Trash ice to be added is lowered the temperature, and temperature will be stablized in the range of 28-30 degree Celsius, observes the color change of worm's ovum, from canescence to
Brown to it is light yellow finally arrive saffron when stop stirring, and at once the switch below shelling bucket is opened, and uses 150 mesh
Receive ovum net and collect worm's ovum;
S6:After the worm's ovum inside shelling bucket has been collected with receipts ovum net, i.e., the bleaching above brine shrimp eggs is will be attached to fresh water
Water is cleaned up;
S7:After the shelling ovum for cleaning up is drained the water, an amount point egg hatching on demand, during hatching, 56 purpose of standby net of hatching is carried out
Hatching 24 hours.
2. it is according to claim 1 it is efficient improve brine shrimp eggs incubation rate preprocess method, it is characterised in that:The step
The process of ovum is steeped in rapid S1, specifically:Measure according to demand first, according to one jin of ovum with the ratio of five liters of fresh water soaking 1 hour,
Wherein, if the fresh water for using is running water, need first to detect whether with chlorine residue, can just use without chlorine residue.
3. it is according to claim 1 it is efficient improve brine shrimp eggs incubation rate preprocess method, it is characterised in that:The step
When adding trash ice in rapid S2, can be with by the way of stirring when trash ice is added, and real-time monitoring temperature treats that temperature reaches 21 degree
When, stop stirring and on the rocks.
4. it is according to claim 1 it is efficient improve brine shrimp eggs incubation rate preprocess method, it is characterised in that:The step
Suddenly the compound method of the dejacketing solution in S3 is:Required raw material is 10% bleaching water and 99% flaky sodium hydrate, according to
Production requirement, carries out mixed preparing with the ratio of 1.5 liters of bleaching waters and 60 grams of NaOH according to 1 jin of worm's ovum, when preparation
Need to wear sour gas anti-poison respirator, and stir flaky sodium hydrate to being completely dissolved.
5. the preprocess method of the efficient raising brine shrimp eggs incubation rate according to claim 1-4 any one claim,
It is characterized in that:After the ovum that shells in step S6 is accepted with the receipts ovum net of 150 mesh, need totally to be adhered to fresh water flush
In the bleaching water on shelling ovum surface, clean level is tested with chlorine residue kit, and chlorine residue is judged to clean up when being 0mg/L.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111466322A (en) * | 2020-04-01 | 2020-07-31 | 中国水产科学研究院南海水产研究所热带水产研究开发中心 | Method for shelling fairy shrimp resting eggs |
CN113508763A (en) * | 2021-04-21 | 2021-10-19 | 安徽农业大学 | Indoor breeding method of Taishi goldfish |
CN114651764A (en) * | 2022-03-21 | 2022-06-24 | 天津农学院 | Method for rapidly identifying quality of newly-laid eggs of fairy shrimp |
CN115399265A (en) * | 2022-09-01 | 2022-11-29 | 海南慈德高科技渔业有限公司 | Method for hatching artemia cysts |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111466322A (en) * | 2020-04-01 | 2020-07-31 | 中国水产科学研究院南海水产研究所热带水产研究开发中心 | Method for shelling fairy shrimp resting eggs |
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CN114651764A (en) * | 2022-03-21 | 2022-06-24 | 天津农学院 | Method for rapidly identifying quality of newly-laid eggs of fairy shrimp |
CN115399265A (en) * | 2022-09-01 | 2022-11-29 | 海南慈德高科技渔业有限公司 | Method for hatching artemia cysts |
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Application publication date: 20170322 |