CN106501514A - Detection reagent for alanine aminotransferase box - Google Patents
Detection reagent for alanine aminotransferase box Download PDFInfo
- Publication number
- CN106501514A CN106501514A CN201610895043.XA CN201610895043A CN106501514A CN 106501514 A CN106501514 A CN 106501514A CN 201610895043 A CN201610895043 A CN 201610895043A CN 106501514 A CN106501514 A CN 106501514A
- Authority
- CN
- China
- Prior art keywords
- reagent
- alanine aminotransferase
- detection
- stabilizer
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to alanine aminotransferase (ALT) detection kit, belongs to clinical in vitro diagnosis in vitro reagent technique field.The purpose of the present invention is to provide the Detection reagent for alanine aminotransferase box for effectively eliminating the interference of endogenouss alpha Ketoglutarate and having good stability, and makes the result of measure alanine aminotransferase more accurately and reliably.The test kit of the present invention includes reagent 1 and reagent 2：The component of wherein reagent 1 includes：Tris HCl buffer, L alanine, reduced coenzyme (NADH), lactic acid dehydrogenase (LDH), enzymatic protective reagent, stabilizer, preservative；The component of reagent 2 includes：Tris HCl buffer, alpha Ketoglutarate, stabilizer, preservative.ALT detection kit provided by the present invention adds enzymatic protective reagent, stabilizer, improves the stability of Detection reagent for alanine aminotransferase box, it is adaptable to all kinds of automatic clinical chemistry analyzers, simple to operate, safety, is easy to clinical practice to promote.
Description
Technical field：
The invention belongs to clinical in vitro diagnosis in vitro reagent technique field, is related to Detection reagent for alanine aminotransferase box.
Background technology：
Alanine aminotransferase (ALT) is the aminotransferase that can be catalyzed between alanine and α-ketoglutaric acid, is
One kind participates in the metabolic enzyme of human body protein, plays a part of quickening people's vivo protein and converts in vivo, and the enzyme is extensive
It is present in the various tissues of human body, organ, muscle, skeleton, with most in the cytoplasm of liver cell.Once there is hepatocyte to suffer
Infringement, liver function decline, hepatic necrosis, intracellular ALT can be discharged into extracellular, so as to cause serum alt content
Raise.ALT activity is raised and indicates hepatocyte injury, also seen in cardiac muscle and Skeletal muscle injury.Serum ALT activities are hepatocyte
The important symbol of the sensitive indicator of damage, especially viral hepatitis, current ALT are clinically mainly used to detect liver function.
The main You Laishi colorimetrys of the method for vitro detection alanine aminotransferase (ALT), Pyruvate oxidase method and
Continuous monitoring method (performance rate method).The shortcoming of Lai Shi colorimetrys is easily to be affected by the feedback suppression of product acetone acid, causes to survey
The accuracy and precision for determining result be not high；Although Pyruvate oxidase method measurement result is preferably, 4- amino peace used is replaced
More poisonous than woods, have teratogenecity, be unsatisfactory for green principle；The advantage of continuous monitoring method is to can determine the linear phase and calculate per minute
The change (Δ A/min) of absorbance, calculates enzymatic activity again exactly according to this value, it is possible to use automatic clinical chemistry analyzer is carried out
Detection；The specificity of enzyme and substrate reactions is good, and testing result accuracy is high.Three of the above method compares, using continuous monitoring
Method is preferable.
The test kit of the present invention is mainly detected using continuous monitoring method that its Cleaning Principle is：ALT is catalyzed L-Alanine
Transamination to α-ketoglutaric acid, generate acetone acid and L-Glutamic Acid.It is same that lactic acid dehydrogenase (LDH) catalysis acetone acid is reduced
When reduced coenzyme (NADH) is oxidized to oxidized coenzyme (NAD+), as NADH has characteristic absorption at 340nm, so
The vigor of the fall off rate and ALT of NADH is directly proportional.Reaction equation is as follows：
Pyruvate oxidase method, such as 103320497 B of CN (2015.09.09), CN are adopted ALT detection kit at present more
100595282 C (2010.03.24), 1086205 C of CN (2002.06.12) adopt this method；CN 104195220 A
(2014.12.10) impact of the buffer to ALT reagents is mainly talked about；104404127 A of CN (2015.03.11) the ALT examinations
Agent box adopts single agents, it is impossible to eliminate endogenouss interference.Detection reagent for alanine aminotransferase box according to the present invention is adopted
With liquid double reagent, endogenouss interference can be effectively eliminated, without the need for Sample pretreatment, can directly using automatic clinical chemistry analyzer
High-volume pattern detection is carried out, simple to operate, accuracy is high, good stability, be suitable for clinical practice and promote.
Content of the invention：
It is an object of the invention to provide Detection reagent for alanine aminotransferase box supplies clinical practice, mainly solve existing
Accuracy that ALT detection fields are present is low, cumbersome, pollution environment, the problems such as reagent is unstable.
In order to solve the interference that endogenouss α-ketoglutaric acid of the present invention is determined to alanine (ALT), using following technical side
Case：Detect using two-step method, serum is mixed with the substrate solution of (lacking α-ketoglutaric acid) that 37 DEG C of insulation 5min are made in sample
The side reaction that contained endogenouss α-ketoglutaric acid causes is finished.The catalytic reaction that α-ketoglutaric acid starts ALT is subsequently adding,
Continuous monitoring absorbance fall off rate at the dominant wavelength 340nm, according to linear response phase absorbance fall off rate (- Δ A/min),
Calculate ALT vigor.
The test kit that the present invention is provided is made up of reagent 1 and reagent 2, and the composition and composition of wherein reagent 1 is as follows：
Tris-HCl buffer 120mmol/L-180mmol/L, PH=8.4
L-Alanine 650mmol/L-850mmol/L
Reduced coenzyme (NADH) 0.2mmol/L-0.4mmol/L
Lactic acid dehydrogenase (LDH) 2000U/L-3000U/L
Enzymatic protective reagent
Stabilizer
Preservative
The composition and composition of reagent 2 is as follows：
Tris-HCl buffer 60mmol/L-100mmol/L, PH=7.2
α-ketoglutaric acid 35mmol/L-55mmol/L
Stabilizer
Preservative
One or more compositionss of the enzymatic protective reagent in bovine serum albumin (BSA) or glycerol and saccharide, its
Consumption is BSA0.1%-2%, glycerol 5%-20%, preferably BSA 0.5%-1.5%, glycerol 10%-15%.
The above saccharide is selected from one or more in glucose, sucrose, maltose, Fructose, and its consumption is 1g/L-
15g/L, preferably 5g/L-10g/L.
The stabilizer of the reagent 1 and reagent 2 is selected from KCl and surface activator composition, and its consumption is KCl 1g/L-
15g/L, preferably 5g/L-10g/L.
The above surfactant is selected from tween 20 (Tween-20), tween 80 (Tween-80), TritonX X-
One kind or many in 100 (Triton X-100), polyethylene glycol 6000 (PEG 6000) and Macrogol 8000 (PEG 8000)
Kind, its consumption is 0.1%-2%, preferably 0.5%-1.5%.
The preservative of the reagent 1 and reagent 2 is selected from NaN3Or Proclin-300, its consumption is NaN30.1g/L-
2g/L, Proclin-3000.1%-2%, preferably NaN30.6g/L-1g/L, Proclin-3000.5%-1%.
Alanine aminotransferase (ALT) of the present invention detection kit be applied to all kinds of automatic clinical chemistry analyzers, now in
Application on automatic clinical chemistry analyzer BS-420, its specifically used method are as follows：
1 pattern detection operation sequence of table
Computational methods：ALT contents (U/L) in sample=Δ AT/min × F
ΔAT/min：Pipe average minute clock is determined with respect to blank tube absorbance change
Note：TV：Reagent sample cumulative volume, SV are sample volume；6.22：MM molecule extinctions of the NADH at 340nm
Coefficient；
1.0：Cuvette optical path (cm)；1000：The conversion coefficient of U/mL to U/L.
Quality-control product used in the present invention is the high and low value quality-control product of Roche；Institute's test sample product are not haemolysis serum.
Beneficial effect：Detection reagent for alanine aminotransferase box of the present invention is liquid double reagent, using two-step method, effectively
The interference of endogenouss α-ketoglutaric acid is eliminated, the accuracy of ALT measure is improve；Enzymatic protective reagent and steady is added in test kit of the present invention
Determine agent, improve the stability of ALT test kits, can stablize at least one week in 37 DEG C of water-baths, illustrate that 2-8 DEG C of preservation can be at least steady
Fixed 1 year.
The present invention is further illustrated below in conjunction with specific embodiment, it should be appreciated that specific embodiment is merely to illustrate this
Bright, and the unrestricted present invention.
Description of the drawings：
Fig. 1 is the blank absorbency change contrast test that 2-8 DEG C of embodiment of the present invention 1-3 and comparative example preserve a week
Result figure；
Fig. 2 is 37 DEG C of water-baths of embodiment of the present invention 1-3 and comparative example blank absorbency of week change contrast test
Result figure.
Specific embodiment
Embodiment 1
Detection reagent for alanine aminotransferase box, is made up of reagent 1 and reagent 2, and concrete composition and content are as follows：
The composition of reagent 1：
Tris-HCl buffer 120mmol/L, PH=8.4
L-Alanine 650mmol/L
NADH 0.2mmol/L
LDH 2000U/L
KCl 5g/L
Triton x-100 1%
Glycerol 15%
Sucrose 5g/L
NaN31g/L
The composition of reagent 2：
Tris-HCl buffer 100mmol/L, PH=7.2
α-ketoglutaric acid 35mmol/L
KCl 6g/L
PEG 8,000 1.5%
NaN30.6g/L
Embodiment 2
Detection reagent for alanine aminotransferase box, is made up of reagent 1 and reagent 2, and concrete composition and content are as follows：
The composition of reagent 1：
Tris-HCl buffer 150mmol/L, PH=8.4
L-Alanine 750mmol/L
NADH 0.3mmol/L
LDH 2500U/L
KCl 8g/L
Tween 20 1%
BSA 1.5%
Maltose 10g/L
Proclin-300 0.8%
The composition of reagent 2：
Tris-HCl buffer 80mmol/L, PH=7.2
α-ketoglutaric acid 45mmol/L
KCl 8g/L
PEG 6,000 1%
Proclin-300 0.5%
Embodiment 3
Detection reagent for alanine aminotransferase box, is made up of reagent 1 and reagent 2, and concrete composition and content are as follows：
The composition of reagent 1：
Tris-HCl buffer 180mmol/L, PH=8.4
L-Alanine 850mmol/L
NADH 0.4mmol/L
LDH 3000U/L
KCl 10g/L
Tween 80 1.5%
PEG 8,000 0.5%
Glycerol 10%
Fructose 6g/L
Glucose sugar 8g/L
Proclin-300 1%
The composition of reagent 2：
Tris-HCl buffer 60mmol/L, PH=7.2
α-ketoglutaric acid 55mmol/L
KCl 10g/L
Triton x-100 0.5%
PEG 6,000 0.5%
Proclin-300 0.8%
Comparative example
The Detection reagent for alanine aminotransferase box of stabilizer and enzymatic protective reagent is not added with, is made up of reagent 1 and reagent 2,
Concrete composition and content are as follows：
The composition of reagent 1：
Tris-HCl buffer 180mmol/L, PH=8.4
L-Alanine 850mmol/L
NADH 0.4mmol/L
LDH 3000U/L
NaN31g/L
The composition of reagent 2：
Tris-HCl buffer 60mmol/L, PH=7.2
α-ketoglutaric acid 55mmol/L
NaN30.6g/L
Embodiment of the present invention 1-3 and comparative example are carried out accuracy validation test.Quality-control product selects Roche high level matter
Control (ALT target value：118 U/L, target value scope (± 10%)：106.2U/L-129.8U/L)；Roche low value Quality Control (ALT target value：
37U/L, target value scope (± 10%)：33.3U/L-40.7U/L).Detected have using BS-420 automatic clinical chemistry analyzers
, as described in table one, after being calibrated using One point standard method, ALT measured values and Blank absorbance values can be straight from instrument for body operational approach
Reading is connect, in order to reduce incidental error, is repeated three times and is taken average.
Embodiment 1-3 and comparative example's accuracy result are as follows：
2 embodiment 1-3 of table and comparative example's accuracy compare
Embodiment of the present invention 1-3 and comparative example are carried out stability checking test.Will be every kind of for above four kinds of test kits
Two parts are divided into, a as 2-8 DEG C of preservation, another preserves as in 37 DEG C of water-baths, every other day determines once, continuously
Monitoring one week, the change of observation blank absorbency and determination of serum value (serum definite value 75.6U/L, it is allowed to range of error (± 10%)：
68.04U/L-83.16U/L), reagent stability is compared by the change of blank absorbency and the measure of accuracy.
3 embodiment of table, 1 stabilization of kit data
4 embodiment of table, 2 stabilization of kit data
5 embodiment of table, 3 stabilization of kit data
6 comparative example's stabilization of kit data of table
Test kit accuracy obtained in embodiment 1-3 is interior in the range of error (± 10%) for allowing as shown in Table 2, and right
Than test kit accuracy obtained in embodiment beyond error allowed band：Embodiment 1-3 be can be seen that by Fig. 1 and Fig. 2 to be obtained
The stability of test kit be significantly stronger than test kit obtained in comparative example.Although test kit reagent obtained in embodiment 1-3
Blank has a certain degree of decline, but the amplitude for declining is less, in allowed band, illustrates to add suitable stabilizer and enzyme
Protective agent can slow down the activity of the decay of NADH, protective enzyme, and then the stability of raising test kit, extend the preservation of test kit
Phase.
Claims (6)
1. Detection reagent for alanine aminotransferase box, is made up of reagent 1 and reagent 2, it is characterised in that
The composition and composition of reagent 1 is as follows：
Tris-HCl buffer 120mmol/L-180mmol/L, PH=8.4
L-Alanine 650mmol/L-850mmol/L
Reduced coenzyme (NADH) 0.2mmol/L-0.4mmol/L
Lactic acid dehydrogenase (LDH) 2000U/L-3000U/L
Enzymatic protective reagent
Stabilizer
Preservative
The composition and composition of reagent 2 is as follows：
Tris-HCl buffer 60mmol/L-100mmol/L, PH=7.2
α-ketoglutaric acid 35mmol/L-55mmol/L
Stabilizer
Preservative.
2. Detection reagent for alanine aminotransferase box as claimed in claim 1, it is characterised in that the enzymatic protective reagent is selected from
Bovine serum albumin (BSA) or glycerol and one or more compositions in saccharide, its consumption is BSA 0.5%-1.5%, sweet
Oily 10%-15%.
3. saccharide as claimed in claim 2, it is characterised in that the one kind being selected from glucose, sucrose, maltose, Fructose
Or multiple, its consumption is 5g/L-10g/L.
4. Detection reagent for alanine aminotransferase box as claimed in claim 1, it is characterised in that the reagent 1 and reagent 2
Stabilizer be selected from KCl and surface activator composition, wherein KCl consumptions are 5g/L-10g/L.
5. surfactant as claimed in claim 4, it is characterised in that be selected from tween 20 (Tween-20), tween 80
(Tween-80), triton x-100 (Triton X-100), polyethylene glycol 6000 (PEG 6000) and Macrogol 8000 (PEG
8000) one or more in, its consumption are 0.5%-1.5%.
6. Detection reagent for alanine aminotransferase box as claimed in claim 1, it is characterised in that the reagent 1 and reagent 2
Preservative be selected from NaN3Or Proclin-300, its consumption is NaN30.6g/L-1g/L, Proclin-300 0.5%-
1%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610895043.XA CN106501514A (en) | 2016-10-03 | 2016-10-03 | Detection reagent for alanine aminotransferase box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610895043.XA CN106501514A (en) | 2016-10-03 | 2016-10-03 | Detection reagent for alanine aminotransferase box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106501514A true CN106501514A (en) | 2017-03-15 |
Family
ID=58293989
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610895043.XA Pending CN106501514A (en) | 2016-10-03 | 2016-10-03 | Detection reagent for alanine aminotransferase box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106501514A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107356544A (en) * | 2017-07-19 | 2017-11-17 | 王贤俊 | A kind of fatty enzyme detection kit |
| CN107449746A (en) * | 2017-07-19 | 2017-12-08 | 王贤俊 | A kind of preparation method of fatty enzyme detection kit |
| CN107589109A (en) * | 2017-11-08 | 2018-01-16 | 北京北检·新创源生物技术有限公司 | A kind of stable reagent for being used to determine ALT |
| CN107815488A (en) * | 2017-11-08 | 2018-03-20 | 北京北检·新创源生物技术有限公司 | A kind of stable reagent for being used to determine alpha hydroxyl butyric dehydrogenase |
| CN107860729A (en) * | 2017-11-08 | 2018-03-30 | 北京北检·新创源生物技术有限公司 | A kind of stable reagent for being used to determine glutamic-oxalacetic transaminease |
| CN108048526A (en) * | 2017-12-22 | 2018-05-18 | 济南风际生物科技有限公司 | A kind of Detection reagent for alanine aminotransferase box |
| CN108845140A (en) * | 2018-05-04 | 2018-11-20 | 湖北科技学院 | Kit for determining alanine aminopherase |
| CN110387398A (en) * | 2019-07-18 | 2019-10-29 | 浙江蓝怡医药有限公司 | A kind of alanine aminotransferase reagent and its preparation method and application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1231341A (en) * | 1998-04-03 | 1999-10-13 | 北京远东协和生物技术有限公司 | Kit for determining alanine aminopherase |
| EP1484414A1 (en) * | 2002-03-12 | 2004-12-08 | Mitsubishi Kagaku Iatron, Inc. | Reagent for measuring alanine aminotransferase activity |
| CN1800852A (en) * | 2005-01-06 | 2006-07-12 | 上海复旦张江生物医药股份有限公司 | Improved clinical diagnosis reagent for detection by rate method |
| CN104195220A (en) * | 2014-08-14 | 2014-12-10 | 苏州康铭诚业医用科技有限公司 | Buffer solution for human serum alanine aminotransferase measurement |
| CN104404127A (en) * | 2014-11-28 | 2015-03-11 | 山东博科生物产业有限公司 | Blood alanine aminotransferase detecting reagent with high stability |
-
2016
- 2016-10-03 CN CN201610895043.XA patent/CN106501514A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1231341A (en) * | 1998-04-03 | 1999-10-13 | 北京远东协和生物技术有限公司 | Kit for determining alanine aminopherase |
| EP1484414A1 (en) * | 2002-03-12 | 2004-12-08 | Mitsubishi Kagaku Iatron, Inc. | Reagent for measuring alanine aminotransferase activity |
| CN1800852A (en) * | 2005-01-06 | 2006-07-12 | 上海复旦张江生物医药股份有限公司 | Improved clinical diagnosis reagent for detection by rate method |
| CN104195220A (en) * | 2014-08-14 | 2014-12-10 | 苏州康铭诚业医用科技有限公司 | Buffer solution for human serum alanine aminotransferase measurement |
| CN104404127A (en) * | 2014-11-28 | 2015-03-11 | 山东博科生物产业有限公司 | Blood alanine aminotransferase detecting reagent with high stability |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107356544A (en) * | 2017-07-19 | 2017-11-17 | 王贤俊 | A kind of fatty enzyme detection kit |
| CN107449746A (en) * | 2017-07-19 | 2017-12-08 | 王贤俊 | A kind of preparation method of fatty enzyme detection kit |
| CN107356544B (en) * | 2017-07-19 | 2021-07-06 | 王贤俊 | Lipase detect reagent box |
| CN107589109A (en) * | 2017-11-08 | 2018-01-16 | 北京北检·新创源生物技术有限公司 | A kind of stable reagent for being used to determine ALT |
| CN107815488A (en) * | 2017-11-08 | 2018-03-20 | 北京北检·新创源生物技术有限公司 | A kind of stable reagent for being used to determine alpha hydroxyl butyric dehydrogenase |
| CN107860729A (en) * | 2017-11-08 | 2018-03-30 | 北京北检·新创源生物技术有限公司 | A kind of stable reagent for being used to determine glutamic-oxalacetic transaminease |
| CN107860729B (en) * | 2017-11-08 | 2022-03-11 | 北京北检·新创源生物技术有限公司 | Stable reagent for determining glutamic-oxalacetic transaminase |
| CN108048526A (en) * | 2017-12-22 | 2018-05-18 | 济南风际生物科技有限公司 | A kind of Detection reagent for alanine aminotransferase box |
| CN108845140A (en) * | 2018-05-04 | 2018-11-20 | 湖北科技学院 | Kit for determining alanine aminopherase |
| CN110387398A (en) * | 2019-07-18 | 2019-10-29 | 浙江蓝怡医药有限公司 | A kind of alanine aminotransferase reagent and its preparation method and application |
| CN110387398B (en) * | 2019-07-18 | 2023-12-19 | 浙江蓝怡医药有限公司 | Alanine aminotransferase reagent and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106501514A (en) | Detection reagent for alanine aminotransferase box | |
| CN102154443B (en) | Creatine jubase MB isozyme activity detection reagent and preparation method thereof | |
| CN102507916B (en) | Ischemia modified albumin liquid stabilization kit | |
| CN106644976A (en) | Aspartate amino transferase detection kit | |
| CN105203472A (en) | Stable NEFA (non-esterified fatty acid) measuring kit | |
| CN106124779A (en) | A kind of test kit for measuring creatinine and method | |
| CN111321198B (en) | Glutathione reductase assay kit and preparation method and application thereof | |
| CN103760357A (en) | Detection kit for ischemia modified albumin | |
| CN104359906B (en) | A kind of stabilization, the serum tolal bile acid detection reagent of strong antijamming capability | |
| CN108287233A (en) | A kind of enzyme process uric acid detection reagent of strong antijamming capability | |
| CN104483487A (en) | Kit for detecting 1, 5-anhydro-D-ghlcitol in human blood | |
| CN106367472A (en) | Kit and method for determining uric acid | |
| CN104195222B (en) | A kind of compound stabilizer for total cholesterol assay kit | |
| CN106290202A (en) | A kind of preparation method of reagent thereof using continuous monitoring method quantitative determination AST | |
| CN101498662A (en) | Reagent kit for monoamine oxidase MAO single-reagent measurement | |
| CN106868096A (en) | A kind of stability is strong and hexokinase method glucose determination reagent of low cost | |
| CN104198422A (en) | Composite stabilizer for alpha-hydroxybutyric dehydrogenase assay kit | |
| Jelikić-Stankov et al. | Determination of uric acid in human serum by an enzymatic method using N-methyl-N-(4-aminophenyl)-3-methoxyaniline reagent | |
| CN106501194A (en) | A kind of employing continuous monitoring method quantitative determines the preparation method of reagent thereof of ALT | |
| CN102994612A (en) | Liquid monomer reagent for determining content of ammonia in blood serum, preparation method and application thereof | |
| Brurok et al. | Effects of MnDPDP, DPDP--, and MnCl2 on cardiac energy metabolism and manganese accumulation: an experimental study in the isolated perfused rat heart | |
| CN108690868A (en) | A kind of adenosine deaminase assay kit and its assay method | |
| Brindle et al. | A 1H‐NMR study of the activity expressed by lactate dehydrogenase in the human erythrocyte | |
| CN102262066A (en) | Method and kit for detecting monoamine oxidase content | |
| CN110343740A (en) | A kind of highly sensitive Plasma lactate method and lactate acid detection kit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170315 |