CN106497785A - A kind of Universal Cell culture dish - Google Patents
A kind of Universal Cell culture dish Download PDFInfo
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- CN106497785A CN106497785A CN201610845230.7A CN201610845230A CN106497785A CN 106497785 A CN106497785 A CN 106497785A CN 201610845230 A CN201610845230 A CN 201610845230A CN 106497785 A CN106497785 A CN 106497785A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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Abstract
The invention discloses a kind of Universal Cell culture dish, belongs to biological consumptive material field.A kind of Universal Cell culture dish of the present invention, including ware box and camera lens, the ware box and camera lens are detachably connected by connector, the ware cassette bottom portion is made up of high light transmissive material, the thickness in the ware cassette bottom portion is less than 0.7mm, the ware cassette bottom portion is covered with flourescent sheet, and the thickness of the flourescent sheet is 0.3mm.The present invention a kind of Universal Cell culture dish have disclosure satisfy that the micro- requirement of high power, integrate cultured cells and observe, can be observed while culture, cell body ingress of air is avoided to affect cell function, can facilitate in observation simultaneously and nutrient solution is suctioned out, reduce and observe the characteristics of affecting.
Description
Technical field
The present invention relates to a kind of culture dish, particularly a kind of Universal Cell culture dish.
Background technology
In view of extensive application of the microscopy in Basic of Biology research and medical industry, various living cells show
Micro mirror imaging technique becomes the important tool in biomedical research.Go for high-quality, high-resolution living cells microscope
The parameters such as image, the consumptive material thickness of its cultured cells, refraction coefficient are extremely important, especially go for high-resolution living cells
Micro-image, it is often necessary to which the microscope using high power is first-class, the now requirement to Universal Cell culture dish base material, especially
Be thickness and refraction coefficient requirement very harsh.The correlations such as cell culture consumptive material common at present, the thickness of its base material
Parameter is unsatisfactory for the demand of high-resolution imaging so that carry out high-resolution living cells imaging process become extremely complex；Often
After needing first to cultivate cell, then cell is moved on slide, then slide is fixed in imaging systems, fiber is allowed
Camera lens can be imaged directly facing slide, in this course, may be revealed along with nutrient solution, and slide may be sudden and violent
Reveal time course in atmosphere and affect cell function, cell function will may be affected during cell body immigration slide
The shortcomings of.
Content of the invention
The goal of the invention of the present invention is：For above-mentioned problem, there is provided a kind of disclosure satisfy that high power micro- will
Ask, integrate cultured cells and observe, can be observed while culture, it is to avoid cell body ingress of air affects cell
Function, while can facilitate in observation suctioning out nutrient solution, reduces the Universal Cell culture dish that observation affects.
The technical solution used in the present invention is as follows：
A kind of Universal Cell culture dish of the present invention, including ware box, the ware box is detachably connected with connector, the ware cassette bottom
Portion is made up of high light transmissive material, and the thickness in the ware cassette bottom portion is less than 0.7mm, and the ware cassette bottom portion is covered with flourescent sheet, described glimmering
The thickness of mating plate is 0.3mm..
As a result of above-mentioned technical proposal, ware box is detachably connected with microscopical camera lens by connector, you can
Ware box is connected with camera lens by connector when needing to observe, ware cassette bottom portion is made up of high light transmissive material, it is seen that light
Transmitance is 98.43%, while the toughness in incubation to vessel requirement is disclosure satisfy that, can be further by flourescent sheet
To observing gain, High Accuracy Observation demand is met.
A kind of Universal Cell culture dish of the present invention, the ware box surface are evenly equipped with some culture tanks, and the culture tank is in
Rectangular array is arranged, and the culture tank is rounded, and the horizontal and vertical upper two adjacent culture tank is by connecting canal connection, institute
State the depth of the depth less than culture tank of connection canal.
As a result of above-mentioned technical proposal, cell culture is carried out in culture tank, connection canal can will ensure nutrient solution
Flowing in whole cultivation region, meanwhile, if nutrient solution is impacted to observation, nutrient solution can all be inhaled in fixed point
/ injection is played, reduces the destruction during picking up/injecting nutrient solution to cell.
A kind of Universal Cell culture dish of the present invention, the culture tank positioned at array edges are transversely connected with longitudinal direction
Connect canal, the spacing >=0.02cm between two culture tank, the depth of the culture tank is 0.47cm, the depth of the connection canal
≤0.21cm.
As a result of above-mentioned technical proposal, nutrient solution can be picked up/be injected in edge fixed point batch, will not be to training
Damage in cell in foster groove so that observed result is more accurate, connect the depth of the depth much smaller than culture tank of canal, energy
During enough avoiding cell culture, cell overflows and is grown in connecting in canal, affects observation.
A kind of Universal Cell culture dish of the present invention, is connected with gain piece, the gain piece between the connector and ware box
Thickness including the photic zone one being linked in sequence from top to bottom, gain layer and photic zone two, the photic zone one and photic zone two
For 0.08cm, the thickness of the gain layer is 1.22mm, and the photic zone one and photic zone two are made by high light transmissive material.
As a result of above-mentioned technical proposal, gain layer can increase the degree of accuracy of observation to carrying out gain with regard to light, full
The observation condition that sufficient High Accuracy Observation needs.
A kind of Universal Cell culture dish of the present invention, is provided with stepped hole in the middle part of the connector, and the stepped hole includes
Hole and lower opening, the diameter in the upper hole are equal to the diameter of gain piece；The upper hole inwall is provided with profile of tooth buckling groove,
The gain piece bottom is provided with clamping engagement groove；The gain piece and ware box are flexibly connected by screw thread.
As a result of above-mentioned technical proposal, the distance of distance of camera lens ware box is substantially reduced, the effect of gain piece is obtained
Farthest play, meanwhile, in observation, the space between ware box, gain piece and camera lens is less, substantially reduce three it
Between air flow, impact of the air to cytoactive is down to minimum；The diameter of lower opening is equal to the diameter of general camera lens
Ware box, when using, can be attached with all purpose microscope camera lens, so as to be observed by size by connector.
A kind of Universal Cell culture dish of the present invention, the hole depth in the upper hole are equal to the thickness of gain piece.
As a result of above-mentioned technical proposal, connector is high with the compatible degree of camera lens and gain piece, ensure that maximum journey
The distance between the reduction ware box of degree and camera lens.
A kind of Universal Cell culture dish of the present invention, the high light transmissive material is by 37 parts of poly-methyl methacrylates of mass parts
Ester, 59 parts of polyamine
13 parts of methacrylic acids, 40 parts of SnO2With 18 parts of Sm2O3Composition, the polyamine n：M is 5：7, the SnO2And Sm2O3Grain
Footpath is less than 700nm.
As a result of above-mentioned technical proposal, the translucency of material is fabulous, it is seen that the transmitance of light can reach 98.43%,
In observation, although have the stop compared with multilayer, but optical attenuation degree is low, therefore, observation effect is good, disclosure satisfy that high accuracy is seen
The demand of survey；Material had good ductility before hardening is dried, and can prepare the light transmissive material of thinner thickness.
A kind of Universal Cell culture dish of the present invention, the gain layer is by 59 parts of polyamine of mass parts
13 parts of methacrylic acids, 5 parts of InGaAs, 0.7 part of Graphene and 7 parts of YVO4Composition, the polyamine n：M is 5：7.
As a result of above-mentioned technical proposal, gain layer has certain gain effect to visible ray, can stimulated radiation,
The intensity of a small amount of amplification visible ray, can make up the light intensity decays that light transmissive material brings.
A kind of Universal Cell culture dish of the present invention, the flourescent sheet is by 71 parts of polyamine of mass parts
20 parts of methacrylic acids, 6 parts of CsBr, 11 parts of TmBr3With 3 parts of PbBr2Composition, the CsBr, TmBr3And PbBr2Crosslinking
Nanocrystal is formed, the particle diameter of the nanocrystal is less than 50nm.
As a result of above-mentioned technical proposal, transient optical state can be presented within the excited by visible light state short time and be entered
Journey, carries out gain to visible ray.
In sum, as a result of above-mentioned technical proposal, the invention has the beneficial effects as follows：
1st, the micro- requirement of high power is disclosure satisfy that, is integrated cultured cells and is observed, can be observed while culture,
Cell body ingress of air is avoided to affect cell function, while can facilitate in observation suctioning out nutrient solution, reducing observation affects.
2nd, the transmitance of visible ray is 98.43%, disclosure satisfy that the toughness to vessel requirement in incubation, to visible
Light has certain gain effect, can stimulated radiation, the intensity of a small amount of amplification visible ray can make up light transmissive material and bring
Light intensity decays, meet the demand of High Accuracy Observation.
Description of the drawings
Fig. 1 is a kind of Universal Cell culture dish structural representation.
Mark in figure：2 is ware box, and 3 is gain piece, and 4 is connector, and 5 is camera lens.
Specific embodiment
Below in conjunction with the accompanying drawings, the present invention is described in detail.
In order that the purpose of invention, technical scheme and advantage become more apparent, below in conjunction with drawings and Examples, to this
Invention is further elaborated.It should be appreciated that specific embodiment described herein is only in order to explain the present invention, not
For limiting the present invention.
Embodiment 1
As shown in figure 1, a kind of Universal Cell culture dish, including ware box 2, ware box 2 is detachably connected with connector 4,2 bottom of ware box
It is made up of high light transmissive material, the thickness of 2 bottom of ware box is less than 0.7mm, and 2 bottom of ware box is covered with flourescent sheet 1, the thickness of flourescent sheet 1
For 0.3mm.2 surface of ware box is evenly equipped with some culture tanks, and culture tank rectangular array is arranged, and culture tank is rounded, laterally and vertical
Two adjacent culture tanks connect the depth of the depth less than culture tank of canal by connecting canal connection upwards.It is located at array edges
Culture tank is transversely connected with connection canal with longitudinal direction, the spacing >=0.02cm between two culture tanks, and the depth of culture tank is 0.47cm,
Depth≤the 0.21cm of connection canal.Gain piece 3 is connected between connector 4 and ware box 2, and gain piece 3 includes sequentially connecting from top to bottom
The thickness of the photic zone one for connecing, gain layer and photic zone two, photic zone one and photic zone two is 0.08cm, and the thickness of gain layer is
1.22mm, photic zone one and photic zone two are made by high light transmissive material.Stepped hole is provided with the middle part of connector 4, and stepped hole includes
Upper hole and lower opening, the diameter in upper hole are equal to the diameter of gain piece 3, and the diameter of lower opening is equal to all purpose microscope mirror
The diameter of head；Upper hole inwall is provided with profile of tooth buckling groove, and 3 bottom of gain piece is provided with clamping engagement groove；Gain piece 3 and ware box 2 are logical
Cross screw thread flexible connection；Camera lens 5 is flexibly connected with lower opening by screw thread.The hole depth in upper hole is equal to the thickness of gain piece 3.
High light transmissive material is by 37 parts of polymethyl methacrylates of mass parts, 59 parts of polyamine
13 parts of methacrylic acids, 40 parts of SnO2With 18 parts of Sm2O3Composition, polyamine n：M is 5：7, SnO2And Sm2O3Particle diameter be less than
700nm.Gain layer is by 59 parts of polyamine of mass parts
13 parts of methacrylic acids, 5 parts of InGaAs, 0.7 part of Graphene and 7 parts of YVO4Composition, polyamine n：M is 5：7.Flourescent sheet is by matter
Amount 71 parts of polyamine of part
20 parts of methacrylic acids, 6 parts of CsBr, 11 parts of TmBr3With 3 parts of PbBr2Composition, CsBr, TmBr3And PbBr2It is cross-linked to form
Nanocrystal, the particle diameter of nanocrystal are less than 50nm.
Embodiment 2
Polyamine
And the synthetic method of high light transmissive material is as follows：
4 '-diaminodiphenyl ether and with 2,5- bis- (4- aminobenzenes) pyrimidine according to mol ratio 5：7 be well mixed after add there-necked flask
In, in there-necked flask, add appropriate DMAC to cause system solid content is 8% ~ 12%, is passed through excessive nitrogen as shielding gas, heating
5 ~ 8h of backflow, after question response thing is complete, obtains yellow solution, according to both summations and 4,4 '-hexafluoroisopropyli,ene-neighbour's benzene
Dioctyl phthalate mol ratio 1：1.005 add 4 in there-necked flask, and 4 '-hexafluoroisopropyli,ene-phthalic acid continues to be passed through excessive
Nitrogen reacts 5h at room temperature, obtains polyamine glue as shielding gas.
Polymethyl methacrylate, methacrylic acid, nano SnO is added in glue according to mass ratio2With nanometer Sm2O3
After 2 ~ 3h is stirred at room temperature, mixed glue solution casting film-forming on totally smooth glass plate is taken, is existed after 2h is dried at 80 DEG C again
8 ~ 12h, the high light transmission film material of water white transparency is dried under vacuum condition.
Embodiment 3
The preparation method of nanocrystal is as follows：
By Tm3O3It is added in few excessive hydrobromic acid, slow evaporation is concentrated, cooling, suction filtration, after solid sample recrystallization,
Washed with absolute ether, TmBr is obtained after 9 d are placed in silica gel drier3.
CsBr, TmBr are weighed in proportion3And PbBr2After being well mixed with hydrobromic acid, stirring at normal temperature 1d adjustment pH be 3.1 ~
3.4,75 DEG C are to slowly warm up to, after constant temperature stirring 1d, are placed under condition of high voltage（>150MPa）, cooled down according to 7 DEG C/s rapidly
To -30 DEG C, crystal is separated out, at low temperature crystal is filtered, and is washed with absolute ether, obtain nanocrystal.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and
Any modification, equivalent and improvement for being made within principle etc., should be included within the scope of the present invention.
Claims (9)
1. a kind of Universal Cell culture dish, it is characterised in that：Including ware box（2）, the ware box（2）With connector（4）Detachably
Connection, the ware box（2）Bottom is made up of high light transmissive material, the ware box（2）The thickness of bottom is less than 0.7mm, the ware box
（2）Bottom is covered with flourescent sheet（1）, the flourescent sheet（1）Thickness be 0.3mm.
2. a kind of Universal Cell culture dish as claimed in claim 1, it is characterised in that：The ware box（2）If surface is evenly equipped with
Dry culture tank, the culture tank rectangular array arrangement, the culture tank are rounded, the horizontal and vertical upper two adjacent culture
By connecting canal connection, the depth of the connection canal is less than the depth of culture tank to groove.
3. a kind of Universal Cell culture dish as claimed in claim 2, it is characterised in that：It is located at the culture tank of array edges
Connection canal, the spacing >=0.02cm between two culture tank are connected with longitudinal direction transversely, and the depth of the culture tank is
0.47cm, the depth≤0.21cm of the connection canal.
4. a kind of Universal Cell culture dish as claimed in claim 1 or 2, it is characterised in that：The connector（4）With ware box
（2）Between be connected with gain piece（3）, the gain piece（3）Including the photic zone one being linked in sequence from top to bottom, gain layer and printing opacity
Layer two, the thickness of the photic zone one and photic zone two is 0.08cm, and the thickness of the gain layer is 1.22mm, the photic zone
One and photic zone two made by high light transmissive material.
5. a kind of Universal Cell culture dish as claimed in claim 3, it is characterised in that：The connector（4）Middle part is provided with platform
Rank hole, the stepped hole include that hole and lower opening, the diameter in the upper hole are equal to gain piece（3）Diameter；Described
Upper hole inwall is provided with profile of tooth buckling groove, the gain piece（3）Bottom is provided with clamping engagement groove；The gain piece（3）With ware box
（2）It is flexibly connected by screw thread.
6. a kind of Universal Cell culture dish as claimed in claim 5, it is characterised in that：The hole depth in the upper hole is equal to gain piece
（3）Thickness.
7. a kind of Universal Cell culture dish as claimed in claim 6, it is characterised in that：The high light transmissive material is by mass parts 37
Part polymethyl methacrylate, 59 parts of polyamine
13 parts of methacrylic acids, 40 parts of SnO2With 18 parts of Sm2O3Composition, the polyamine n：M is 5：7, the SnO2And Sm2O3Grain
Footpath is less than 700nm.
8. a kind of Universal Cell culture dish as claimed in claims 6 or 7, it is characterised in that：The gain layer is by mass parts 59
Part polyamine
13 parts of methacrylic acids, 5 parts of InGaAs, 0.7 part of Graphene and 7 parts of YVO4Composition, the polyamine n：M is 5：7.
9. a kind of Universal Cell culture dish as claimed in claim 7 or 8, it is characterised in that：The flourescent sheet（1）By mass parts
71 parts of polyamine
20 parts of methacrylic acids, 6 parts of CsBr, 11 parts of TmBr3With 3 parts of PbBr2Composition, the CsBr, TmBr3And PbBr2Crosslinking shape
Into nanocrystal, the particle diameter of the nanocrystal is less than 50nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610845230.7A CN106497785A (en) | 2016-09-24 | 2016-09-24 | A kind of Universal Cell culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610845230.7A CN106497785A (en) | 2016-09-24 | 2016-09-24 | A kind of Universal Cell culture dish |
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| Publication Number | Publication Date |
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| CN106497785A true CN106497785A (en) | 2017-03-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610845230.7A Withdrawn CN106497785A (en) | 2016-09-24 | 2016-09-24 | A kind of Universal Cell culture dish |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1646613A (en) * | 2002-02-19 | 2005-07-27 | 光子－X有限公司 | Polymer nanocomposites for optical applications |
| CN1963663A (en) * | 2006-12-07 | 2007-05-16 | 上海交通大学 | Photography system for welding process of compound heat reservoir in carbon dioxide laser-melting argon-arc welding |
| CN202322860U (en) * | 2011-11-14 | 2012-07-11 | 江苏百奥特医疗仪器科技有限公司 | High-resolution imaged cell culture dish with glass bottom |
| CN102703301A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Darkroom used between installing seat of sequencing chip and CCD (Charge Coupled Device) camera |
| CN202945247U (en) * | 2012-12-07 | 2013-05-22 | 广西壮族自治区妇幼保健院 | Human embryo culture vessel with single culture, co-culture and group culture advantages |
| CN203960183U (en) * | 2014-05-14 | 2014-11-26 | 杨栎 | A kind of darkroom connecting joint for CCD camera |
| CN204125459U (en) * | 2014-10-03 | 2015-01-28 | 亳州师范高等专科学校 | A kind of biological culture laboratory apparatus being convenient to observe |
| US20150344625A1 (en) * | 2014-06-02 | 2015-12-03 | Samsung Electronics Co., Ltd. | Polyimide film, method of preparing polyimide film, optical device including polyimide film |
-
2016
- 2016-09-24 CN CN201610845230.7A patent/CN106497785A/en not_active Withdrawn
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1646613A (en) * | 2002-02-19 | 2005-07-27 | 光子－X有限公司 | Polymer nanocomposites for optical applications |
| CN1963663A (en) * | 2006-12-07 | 2007-05-16 | 上海交通大学 | Photography system for welding process of compound heat reservoir in carbon dioxide laser-melting argon-arc welding |
| CN202322860U (en) * | 2011-11-14 | 2012-07-11 | 江苏百奥特医疗仪器科技有限公司 | High-resolution imaged cell culture dish with glass bottom |
| CN102703301A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Darkroom used between installing seat of sequencing chip and CCD (Charge Coupled Device) camera |
| CN202945247U (en) * | 2012-12-07 | 2013-05-22 | 广西壮族自治区妇幼保健院 | Human embryo culture vessel with single culture, co-culture and group culture advantages |
| CN203960183U (en) * | 2014-05-14 | 2014-11-26 | 杨栎 | A kind of darkroom connecting joint for CCD camera |
| US20150344625A1 (en) * | 2014-06-02 | 2015-12-03 | Samsung Electronics Co., Ltd. | Polyimide film, method of preparing polyimide film, optical device including polyimide film |
| CN204125459U (en) * | 2014-10-03 | 2015-01-28 | 亳州师范高等专科学校 | A kind of biological culture laboratory apparatus being convenient to observe |
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