CN106490523A - A kind of radioprotective spiral algae sheet and preparation method thereof - Google Patents
A kind of radioprotective spiral algae sheet and preparation method thereof Download PDFInfo
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- CN106490523A CN106490523A CN201610882249.9A CN201610882249A CN106490523A CN 106490523 A CN106490523 A CN 106490523A CN 201610882249 A CN201610882249 A CN 201610882249A CN 106490523 A CN106490523 A CN 106490523A
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- sargassum smell
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- 241000195493 Cryptophyta Species 0.000 title claims abstract description 29
- 230000004223 radioprotective effect Effects 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 235000016425 Arthrospira platensis Nutrition 0.000 claims abstract description 132
- 240000002900 Arthrospira platensis Species 0.000 claims abstract description 132
- 229940082787 spirulina Drugs 0.000 claims abstract description 132
- 241000195474 Sargassum Species 0.000 claims abstract description 131
- 239000000843 powder Substances 0.000 claims abstract description 125
- 238000000034 method Methods 0.000 claims abstract description 54
- 230000008569 process Effects 0.000 claims abstract description 39
- 239000002994 raw material Substances 0.000 claims abstract description 21
- 230000005684 electric field Effects 0.000 claims abstract description 20
- 238000005469 granulation Methods 0.000 claims abstract description 19
- 230000003179 granulation Effects 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 238000001035 drying Methods 0.000 claims abstract description 10
- 238000005516 engineering process Methods 0.000 claims abstract description 9
- 239000000835 fiber Substances 0.000 claims abstract description 8
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 6
- 238000009777 vacuum freeze-drying Methods 0.000 claims abstract description 6
- 238000002203 pretreatment Methods 0.000 claims abstract description 3
- 239000012530 fluid Substances 0.000 claims description 56
- 239000007788 liquid Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 108010059892 Cellulase Proteins 0.000 claims description 13
- 229940106157 cellulase Drugs 0.000 claims description 13
- 229940088598 enzyme Drugs 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 238000010926 purge Methods 0.000 claims description 7
- 239000000758 substrate Substances 0.000 claims description 7
- 108010053210 Phycocyanin Proteins 0.000 claims description 6
- 150000004676 glycans Chemical class 0.000 claims description 5
- 229920001282 polysaccharide Polymers 0.000 claims description 5
- 239000005017 polysaccharide Substances 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 2
- 238000002604 ultrasonography Methods 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims 1
- 239000004615 ingredient Substances 0.000 abstract description 8
- 210000002421 cell wall Anatomy 0.000 abstract description 6
- 238000000605 extraction Methods 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 abstract description 3
- 239000000470 constituent Substances 0.000 abstract description 2
- 238000007599 discharging Methods 0.000 abstract description 2
- 238000006911 enzymatic reaction Methods 0.000 abstract description 2
- 230000007062 hydrolysis Effects 0.000 abstract description 2
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 2
- 238000010008 shearing Methods 0.000 abstract description 2
- 238000012546 transfer Methods 0.000 abstract description 2
- 238000002137 ultrasound extraction Methods 0.000 abstract description 2
- 230000000593 degrading effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 12
- 230000000694 effects Effects 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 230000005855 radiation Effects 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 241000192700 Cyanobacteria Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 241001262079 Cutleriales Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241001466077 Salina Species 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003471 anti-radiation Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- VZCCETWTMQHEPK-UHFFFAOYSA-N gamma-Linolensaeure Natural products CCCCCC=CCC=CCC=CCCCCC(O)=O VZCCETWTMQHEPK-UHFFFAOYSA-N 0.000 description 1
- VZCCETWTMQHEPK-QNEBEIHSSA-N gamma-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCC(O)=O VZCCETWTMQHEPK-QNEBEIHSSA-N 0.000 description 1
- 235000020664 gamma-linolenic acid Nutrition 0.000 description 1
- 229960002733 gamolenic acid Drugs 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 201000002364 leukopenia Diseases 0.000 description 1
- 231100001022 leukopenia Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000005453 pelletization Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 235000020995 raw meat Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000011121 sodium hydroxide Nutrition 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
A kind of radioprotective spiral algae sheet and preparation method thereof, pulverized and sieved with 200 280 mesh, the eliminating sargassum smell Spirulina powder of moisture 1% 8% is raw material, drying, sieves, ultrasonic pretreatment, high-voltage pulse electric field technology auxiliary fiber element enzyme Controlled-enzymatic Hydrolysis, concentration, vacuum freeze-drying are dried, and granulation, tablet forming technique process are obtained.Using ultrasonic pretreatment method, local can be made to produce powerful high-speed jet, increased mass-transfer efficiency, the powerful shearing force that ultrasonic cavitation is produced can make medium cell wall rupture, make cell easily discharge inclusions.Make cell breakage simultaneously, be conducive to discharging inclusions.Ultrasonic extraction can improve the yield of effective ingredient, shorten extraction time, simple to operate.Using high-pressure pulse electric auxiliary fiber element enzymolysis method, operating condition is gentle, does not destroy effective ingredient, while using the high specificity of enzyme reaction, by the constituent hydrolysis of cell wall or degrading, destroying cell wall, so as to improve the extraction ratio of effective ingredient.
Description
Technical field
A kind of radioprotective spiral algae sheet and preparation method thereof, is related to spirulina development and application field, is crushed with 200-280 mesh
Sieve, the eliminating sargassum smell Spirulina powder of moisture 1%-8% is raw material, drying is sieved, ultrasonic pretreatment, high-pressure pulse electric
Technology auxiliary fiber element enzyme Controlled-enzymatic Hydrolysis, concentration, vacuum freeze-drying are dried, and granulation, tablet forming technique process are obtained, many in effective ingredient
The yield of sugar is 80%-90%, and the yield of phycocyanin is 70%-75%, and unit tablet quality is 0.5-2.5g with radioprotective work(
The spiral algae sheet of energy.
Background technology
With science and technology and the modernization of people's life, people more and more contact ray, computer. mobile phone, TV
Machine, microwave oven, air-conditioning and armarium etc., radioactivity infringement are increasingly paid attention to.
Spirullina Cyanophyta, Cyanophyceae, section grow Cutleriales, the Spirullina in Oscillariaceae.Usually said spirulina one
As refer to the larger spirulina plalensiss of body and spirulina maxim in Spirullina, be that one kind is distributed in each sea area in the world and land
Algae in light, salina, in aeruginouss.Spirulina has very high nutritive value, containing up to 50 ~ 75% protein,
Multivitamin, it is necessary to aminoacid, it is necessary to fatty acid and mineral etc.；Additionally, in spirulina also containing polysaccharide, phycocyanin,
The biological active substanceies such as gamma-Linolenic acid, FAO (Food and Agriculture Organization of the United Nation) (FAO) are listed spirulina in 21 century human food's resource and are opened
Plan is sent out, and is considered the optimal food of human future.Therefore functional food, health product are developed to more and more
Deng.The 60Co gamma-radiations such as Ruan Jihong irradiate mice, and spirulina polysaccharide albumen has significantly to radiation-induced leukopenia
Improvement（Ruan Jihong, Pang Qishen, Guo Baojiang, etc. the radiation-resistant research of spirulina [J]. heredity, 1988,10 (2)：27.）.
Guo Chunsheng etc.（Guo Chunsheng, Sun Xiaohong, Ge Wei, etc. high dose compound spirulina anti-radiation protection Effect study [J]. Shi Zhen traditional Chinese medical science state
Medicine, 2008,19 (8)：1913-1914.）Test finds that high dose spirulina polysaccharide is with Ginkgo Biloba Extract Application of composite in little
Mus radioprotective has synergistic function, and its effect is better than exclusive use, can be obviously prolonged through 60Co gamma-radiation total irradiation mices
Time-to-live.Zhang Chengwu etc.（Zhang Chengwu, Zeng Shaoqi, Zhang Yuanzhen, etc. blunt top polysaccharides and phycocyanin are put to chmice acute
Penetrate the protection effect [J] of disease. Journal of Nutrition, 1996,18 (3)：327-331.）Report, lumbar injection phycocyanin 125mg/kg
The formation of mice CFU CM and hematopoietic stem cell after 60Co γ-radiation can be stimulated, and increase the number of bone marrow nucleated cell
Amount, the recovery of mice peripheral leukocytes function after enhanced rad.And mouse survival rate can be improved.The research of Wang Yanli etc. is also demonstrate,proved
Real, spirulina polysaccharide can improve the survival rate of raying mice, be effectively increased the relative populations of hematopoietic stem cell.Xuchang is splendid etc.
（Xuchang is splendid. impact [J] of the spirulina to tumor radiotherapy patient's Plasma SOD. Chinese radiological medicine is miscellaneous with protection
Will, 1996, (3)：136-138.）It was found that, phycocyanin may be improved or protect endogenouss SOD, enable preferably to resist
The free radical produced by radiation, allows biomacromolecule from damage, reaches radiation-resistant purpose.
Spirulina Products form in the country's is mainly algae powder at present, or algae powder is added in other food, and product is single, produces
The important effect of product is indefinite, and effective efficiency component content is low, and the comprehensive benefit that Raw material processing is utilized is not high.Using by spirulina
The development and production of the composition such as polysaccharide, phycocyanin are effect, the series helix algae product that purposes is different, such as functional food, can make
Product better adapts to the demand in market, improves the economic benefit of Product processing.
The technical problem to be solved in the present invention is：A kind of radioprotective spiral algae sheet and preparation method thereof is disclosed, prior art is solved
Middle the deficiencies such as spirulina has time-consuming, power consumption, yield is low, the product that obtains is single are processed using hot-water process, cold caustic soda method.There is provided one
Plant rational technology, preparation condition is gentle, effective ingredient is survivable, effective component yield is preferable, the significant radioprotective of feature
Spiral algae sheet and preparation method thereof.
The technical solution used in the present invention is as follows：
1. a kind of radioprotective spiral algae sheet, component contain eliminating sargassum smell Spirulina powder, and described eliminating sargassum smell Spirulina powder is by eliminating sargassum smell spiral
Algae powder raw material is put into and is dried process 20min-60min in 40 DEG C of -80 DEG C of baking ovens, dries terminal for eliminating sargassum smell Spirulina powder water
Point content is down to 1%-8%, then dried eliminating sargassum smell Spirulina powder is carried out 200-280 mesh pulverizes and sieves to obtain.
2. above-mentioned radioprotective spiral algae sheet, unit tablet quality are 0.5-2.5g, and in effective ingredient, the yield of polysaccharide is
80%-90%, the yield of phycocyanin is 70%-75%.
3. the preparation method of the radioprotective spiral algae sheet of the present invention, with eliminating sargassum smell Spirulina powder as raw material, drying is sieved,
Ultrasonic pretreatment, high-voltage pulse electric field technology auxiliary fiber element enzyme Controlled-enzymatic Hydrolysis, concentration, vacuum freeze-drying are dried, granulation, tabletting
Technical process is obtained；
1）Described dry run, is to be put into be dried process in 40 DEG C of -80 DEG C of baking ovens by eliminating sargassum smell Spirulina powder raw material
20min-60min, dries terminal and is down to 1%-8% for eliminating sargassum smell Spirulina powder moisture；
2）Described process of sieving, is that dried eliminating sargassum smell Spirulina powder is carried out 200-280 mesh to pulverize and sieve；
3）Described ultrasonic pretreatment process is eliminating sargassum smell Spirulina powder raw material after drying, 200-280 mesh sieve according to
The feed liquid weight of eliminating sargassum smell Spirulina powder and water and volume 1:10-1:30 carry out batch mixing, after material mix homogeneously, are placed in ultrasound wave
In processing meanss, control ultrasonic power for 100W-800W, ultrasonic time be 5min-35min after, obtain eliminating sargassum smell Spirulina powder pre-
Treatment fluid；
4）Described high-voltage pulse electric field technology auxiliary fiber element enzyme Controlled-enzymatic Hydrolysis process, is that cellulase is added to eliminating sargassum smell spiral shell
In spirulina powder treatment fluid, enzyme is 5%-7% than concentration of substrate, and aids in, with high-pressure pulse electric circulating treatment procedure, using distilled water
With ethanol purge constant flow pump and high-pressure pulse electric feed back pipe 2-5 time, at ambient temperature, by the process of eliminating sargassum smell Spirulina powder
Liquid pumps into high-pressure pulse electric system with the flow velocity of 1.6-4.8 mL/min, opens high-voltage pulse electric field switch, adjusts electric field
Field intensity 5-25 kV/cm and pulse frequency 800-2400HZ, after each run 2-3 min, collect treatment fluid, add cellulase
Treatment fluid pH value 6-10, circular treatment 6-8 time is kept to obtain the process of eliminating sargassum smell Spirulina powder after final regulation treatment fluid pH value 8-11
Liquid；
5）Described concentration process, is that eliminating sargassum smell Spirulina powder treatment fluid is carried out process concentrated in vacuo, and vacuum is 0.065Mpa-
0.095Mpa, temperature are 40 DEG C -85 DEG C, make eliminating sargassum smell Spirulina powder treatment fluid be concentrated to give eliminating sargassum smell Spirulina powder concentrated solution；
6）Described vacuum freeze-drying dry run, is that eliminating sargassum smell Spirulina powder concentrated solution is refrigerated to subzero 25-50 DEG C, then takes out true
Sky heats to obtain eliminating sargassum smell spirulina powder, and wherein evacuation makes pressure be maintained at 100-120Pa, and temperature is maintained at 25-50 DEG C；
7）Described granulation, tableting processes, are by eliminating sargassum smell spirulina powder, cross the granulation of 50-80 mesh sieves by oscillating granulator,
Eliminating sargassum smell spirulina powder after by granulation, tabletting under conditions of temperature 20-22 DEG C, humidity 10-30%, selection pressure is
3000N-4000N, obtains radioprotective eliminating sargassum smell spiral algae sheet.
4. the preparation method of above-mentioned radioprotective eliminating sargassum smell spiral algae sheet, described concentration process, the eliminating sargassum smell spiral after concentration
Algae powder treatment fluid is preferably the 1/2-1/6 of original volume.
The method of the ultrasonic pretreatment that the present invention is adopted, can make local produce powerful high-speed jet, increased mass transfer effect
Rate, the powerful shearing force that ultrasonic cavitation is produced can make medium cell wall rupture, make cell easily discharge inclusions.Make simultaneously thin
Born of the same parents crush, and are conducive to discharging inclusions.Ultrasonic extraction can improve the yield of effective ingredient, shorten extraction time, behaviour
Make simple.Using high-pressure pulse electric auxiliary fiber element enzymolysis method, operating condition is gentle, does not destroy effective ingredient, with
The high specificity of Shi Liyong enzyme reactions, by the constituent hydrolysis of cell wall or degrades, destroys cell wall, so as to improve effectively
The extraction ratio of composition.According to《The function assessment assessment process of health food and method》Through tested 30 days, tested 60 days, tested 90 days
The zoopery of three different batches knows which has anti-radiation effect.
Specific embodiment
Embodiment 1：
Eliminating sargassum smell Spirulina powder raw material is put into process 20min is dried in 40 DEG C of baking ovens, dry to moisture and be down to 1%,
Dried eliminating sargassum smell Spirulina powder is carried out 200 mesh again to pulverize and sieve, the eliminating sargassum smell Spirulina powder after drying, 200 mesh are sieved
Feed liquid weight and volume 1 of the raw material according to eliminating sargassum smell Spirulina powder and water:10 carry out batch mixing, after material mix homogeneously, are placed in super
In sonicated device, control ultrasonic power for 100W, ultrasonic time be 5min after, obtain eliminating sargassum smell Spirulina powder pretreatment fluid；
Again cellulase is added in eliminating sargassum smell Spirulina powder treatment fluid, enzyme is 5% than concentration of substrate, and aids in high-pressure pulse electric
Circulating treatment procedure, with distilled water and ethanol purge constant flow pump and high-pressure pulse electric feed back pipe 2 times, in room temperature condition
Under, eliminating sargassum smell Spirulina powder treatment fluid is pumped into high-pressure pulse electric system with the flow velocity of 1.6 mL/min, high voltage pulse is opened
Electric field is switched, and adjusts 5 kV/cm of electric field strength and pulse frequency 800HZ, after 2 min of each run, collects treatment fluid, is added fine
The plain enzyme of dimension keeps treatment fluid pH value 6, circular treatment 6 times to obtain the process of eliminating sargassum smell Spirulina powder after final regulation treatment fluid pH value 8
Liquid.Eliminating sargassum smell Spirulina powder treatment fluid is carried out process concentrated in vacuo, vacuum is 0.065Mpa, and temperature is 40 DEG C, makes eliminating sargassum smell spiral shell
Spirulina powder treatment fluid is concentrated into the 1/2 of original volume, obtains eliminating sargassum smell Spirulina powder concentrated solution.Eliminating sargassum smell Spirulina powder concentrated solution is freezed
To subzero 25 DEG C, then evacuation heats to obtain eliminating sargassum smell spirulina powder, and wherein evacuation makes pressure be maintained at 100Pa, and temperature is protected
Hold at 25 DEG C；By eliminating sargassum smell spirulina powder, the granulation of 50 mesh sieves is crossed by oscillating granulator, by granulation after eliminating sargassum smell spirulina do
Powder, tabletting under conditions of 20 DEG C of temperature, humidity 10%, selection pressure is 3000N, obtains radioprotective eliminating sargassum smell spiral algae sheet.
Embodiment 2：
Eliminating sargassum smell Spirulina powder raw material is put into process 30min is dried in 50 DEG C of baking ovens, dry to moisture and be down to 2%,
Dried eliminating sargassum smell Spirulina powder is carried out 240 mesh again to pulverize and sieve, the eliminating sargassum smell Spirulina powder after drying, 240 mesh are sieved
Feed liquid weight and volume 1 of the raw material according to eliminating sargassum smell Spirulina powder and water:15 carry out batch mixing, after material mix homogeneously, are placed in super
In sonicated device, control ultrasonic power for 300W, ultrasonic time be 25min after, obtain eliminating sargassum smell Spirulina powder pretreatment fluid；
Again cellulase is added in eliminating sargassum smell Spirulina powder treatment fluid, enzyme is 6% than concentration of substrate, and aids in high-pressure pulse electric
Circulating treatment procedure, with distilled water and ethanol purge constant flow pump and high-pressure pulse electric feed back pipe 4 times, in room temperature condition
Under, eliminating sargassum smell Spirulina powder treatment fluid is pumped into high-pressure pulse electric system with the flow velocity of 3.2 mL/min, high voltage pulse is opened
Electric field is switched, and adjusts electric field strength 15kV/cm and pulse frequency 1100HZ, after 3 min of each run, is collected treatment fluid, is added
Cellulase keeps treatment fluid pH value 8, circular treatment 7 times to obtain at eliminating sargassum smell Spirulina powder after final regulation treatment fluid pH value 10
Reason liquid.Eliminating sargassum smell Spirulina powder treatment fluid is carried out process concentrated in vacuo, vacuum is 0.075Mpa, and temperature is 50 DEG C, makes eliminating sargassum smell
Spirulina powder treatment fluid is concentrated into the 1/4 of original volume, obtains eliminating sargassum smell Spirulina powder concentrated solution.Will be cold for eliminating sargassum smell Spirulina powder concentrated solution
Freeze to subzero 30 DEG C, then evacuation heats to obtain eliminating sargassum smell spirulina powder, wherein evacuation makes pressure be maintained at 110Pa, temperature
It is maintained at 30 DEG C；By eliminating sargassum smell spirulina powder, the granulation of 60 mesh sieves is crossed by oscillating granulator, by granulation after eliminating sargassum smell spirulina
Dry powder, tabletting under conditions of 21 DEG C of temperature, humidity 20%, selection pressure is 3300N, obtains radioprotective eliminating sargassum smell spiral algae sheet.
Embodiment 3：
Eliminating sargassum smell Spirulina powder raw material is put into process 35min is dried in 60 DEG C of baking ovens, dry to moisture and be down to 6%,
Dried eliminating sargassum smell Spirulina powder is carried out 250 mesh again to pulverize and sieve, the eliminating sargassum smell Spirulina powder after drying, 250 mesh are sieved
Feed liquid weight and volume 1 of the raw material according to eliminating sargassum smell Spirulina powder and water:180 carry out batch mixing, after material mix homogeneously, are placed in super
In sonicated device, control ultrasonic power for 450W, ultrasonic time be 28min after, obtain eliminating sargassum smell Spirulina powder pretreatment fluid；
Again cellulase is added in eliminating sargassum smell Spirulina powder treatment fluid, enzyme is 5.7% than concentration of substrate, and aids in high-voltage pulse electric
Circulating treatment procedure, with distilled water and ethanol purge constant flow pump and high-pressure pulse electric feed back pipe 4 times, in room temperature condition
Under, eliminating sargassum smell Spirulina powder treatment fluid is pumped into high-pressure pulse electric system with the flow velocity of 3.6 mL/min, high voltage pulse is opened
Electric field is switched, and adjusts 18 kV/cm of electric field strength and pulse frequency 2100HZ, after 3 min of each run, is collected treatment fluid, is added
Cellulase keeps treatment fluid pH value 7, circular treatment 8 times to obtain the process of eliminating sargassum smell Spirulina powder after final regulation treatment fluid pH value 9
Liquid.Eliminating sargassum smell Spirulina powder treatment fluid is carried out process concentrated in vacuo, vacuum is 0.084Mpa, and temperature is 70 DEG C, makes eliminating sargassum smell spiral shell
Spirulina powder treatment fluid is concentrated into the 1/5 of original volume, obtains eliminating sargassum smell Spirulina powder concentrated solution.Eliminating sargassum smell Spirulina powder concentrated solution is freezed
To subzero 35 DEG C, then evacuation heats to obtain eliminating sargassum smell spirulina powder, and wherein evacuation makes pressure be maintained at 117Pa, and temperature is protected
Hold at 40 DEG C；By eliminating sargassum smell spirulina powder, the granulation of 70 mesh sieves is crossed by oscillating granulator, by granulation after eliminating sargassum smell spirulina do
Powder, tabletting under conditions of 21.5 DEG C of temperature, humidity 18%, selection pressure is 3600N, obtains radioprotective eliminating sargassum smell spiral algae sheet.
Embodiment 4：
Eliminating sargassum smell Spirulina powder raw material is put into process 40min is dried in 60 DEG C of baking ovens, dry and be down to moisture
4.5%, then dried eliminating sargassum smell Spirulina powder is carried out 240 mesh pulverize and sieve, the eliminating sargassum smell spiral after drying, 240 mesh are sieved
Feed liquid weight and volume 1 of the algae powder raw material according to eliminating sargassum smell Spirulina powder and water:20 carry out batch mixing, after material mix homogeneously, put
In ultrasonic treatment unit, control ultrasonic power for 450W, ultrasonic time be 20min after, obtain eliminating sargassum smell Spirulina powder and locate in advance
Reason liquid；Again cellulase is added in eliminating sargassum smell Spirulina powder treatment fluid, enzyme is 6% than concentration of substrate, and aids in high-voltage pulse
Electric field circulating treatment procedure, with distilled water and ethanol purge constant flow pump and high-pressure pulse electric feed back pipe 4 times, in room temperature bar
Under part, eliminating sargassum smell Spirulina powder treatment fluid is pumped into high-pressure pulse electric system with the flow velocity of 3.2 mL/min, open high voltage arteries and veins
Electric field switch, adjustment electric field strength 15kV/cm and pulse frequency 1600HZ is rushed, after 2.5 min of each run, treatment fluid is collected,
Cellulase is added to keep treatment fluid pH value 8, circular treatment 7 times to obtain eliminating sargassum smell spirulina after final regulation treatment fluid pH value 9.5
Powder treatment fluid.Eliminating sargassum smell Spirulina powder treatment fluid is carried out process concentrated in vacuo, vacuum is 0.08Mpa, and temperature is 62.5 DEG C, makes
Eliminating sargassum smell Spirulina powder treatment fluid is concentrated into the 1/4 of original volume, obtains eliminating sargassum smell Spirulina powder concentrated solution.Eliminating sargassum smell Spirulina powder is concentrated
Liquid is refrigerated to subzero 37.5 DEG C, and then evacuation heats to obtain eliminating sargassum smell spirulina powder, and wherein evacuation is maintained at pressure
110Pa, temperature are maintained at 37.5 DEG C；By eliminating sargassum smell spirulina powder, the granulation of 65 mesh sieves is crossed by oscillating granulator, after pelletizing
Eliminating sargassum smell spirulina powder, tabletting under conditions of 21 DEG C of temperature, humidity 20%, selection pressure is 3500N, obtain radioprotective take off
Raw meat spiral algae sheet.
Embodiment 5：
Eliminating sargassum smell Spirulina powder raw material is put into process 60min is dried in 80 DEG C of baking ovens, dry to moisture and be down to 8%,
Dried eliminating sargassum smell Spirulina powder is carried out 280 mesh again to pulverize and sieve, the eliminating sargassum smell Spirulina powder after drying, 280 mesh are sieved
Feed liquid weight and volume 1 of the raw material according to eliminating sargassum smell Spirulina powder and water:30 carry out batch mixing, after material mix homogeneously, are placed in super
In sonicated device, control ultrasonic power for 800W, ultrasonic time be 35min after, obtain eliminating sargassum smell Spirulina powder pretreatment fluid；
Again cellulase is added in eliminating sargassum smell Spirulina powder treatment fluid, enzyme is 7% than concentration of substrate, and aids in high-pressure pulse electric
Circulating treatment procedure, with distilled water and ethanol purge constant flow pump and high-pressure pulse electric feed back pipe 5 times, in room temperature condition
Under, eliminating sargassum smell Spirulina powder treatment fluid is pumped into high-pressure pulse electric system with the flow velocity of 4.8 mL/min, high voltage pulse is opened
Electric field is switched, and adjusts 25 kV/cm of electric field strength and pulse frequency 2400HZ, after 3 min of each run, is collected treatment fluid, is added
Cellulase keeps treatment fluid pH value 10, circular treatment 8 times to obtain at eliminating sargassum smell Spirulina powder after final regulation treatment fluid pH value 11
Reason liquid.Eliminating sargassum smell Spirulina powder treatment fluid is carried out process concentrated in vacuo, vacuum is 0.095Mpa, and temperature is 85 DEG C, makes eliminating sargassum smell
Spirulina powder treatment fluid is concentrated into the 1/6 of original volume, obtains eliminating sargassum smell Spirulina powder concentrated solution.Will be cold for eliminating sargassum smell Spirulina powder concentrated solution
Freeze to subzero 50 DEG C, then evacuation heats to obtain eliminating sargassum smell spirulina powder, wherein evacuation makes pressure be maintained at 120Pa, temperature
It is maintained at 50 DEG C；By eliminating sargassum smell spirulina powder, the granulation of 80 mesh sieves is crossed by oscillating granulator, by granulation after eliminating sargassum smell spirulina
Dry powder, tabletting under conditions of 22 DEG C of temperature, humidity 30%, selection pressure is 4000N, obtains radioprotective eliminating sargassum smell spiral algae sheet.
Claims (4)
1. a kind of radioprotective spiral algae sheet, it is characterised in that component contains eliminating sargassum smell Spirulina powder, and described eliminating sargassum smell Spirulina powder is
Eliminating sargassum smell Spirulina powder raw material is put into process 20min-60min is dried in 40 DEG C of -80 DEG C of baking ovens, dry terminal for eliminating sargassum smell
Spirulina powder moisture is down to 1%-8%, then dried eliminating sargassum smell Spirulina powder is carried out 200-280 mesh pulverizes and sieves to obtain
's.
2. according to the radioprotective spiral algae sheet described in claim 1, it is characterised in that unit tablet quality is 0.5-2.5g, effectively
In composition, the yield of polysaccharide is 80%-90%, and the yield of phycocyanin is 70%-75%.
3. the preparation method of the radioprotective spiral algae sheet described in a kind of claim 1, with eliminating sargassum smell Spirulina powder as raw material, through dry
Dry, to sieve, ultrasonic pretreatment, high-voltage pulse electric field technology auxiliary fiber element enzyme Controlled-enzymatic Hydrolysis, concentration, vacuum freeze-drying are dried,
Granulation, tablet forming technique process are obtained；
1）Described dry run, is to be put into be dried process in 40 DEG C of -80 DEG C of baking ovens by eliminating sargassum smell Spirulina powder raw material
20min-60min, dries terminal and is down to 1%-8% for eliminating sargassum smell Spirulina powder moisture；
2）Described process of sieving, is that dried eliminating sargassum smell Spirulina powder is carried out 200-280 mesh to pulverize and sieve；
3）Described ultrasonic pretreatment process is eliminating sargassum smell Spirulina powder raw material after drying, 200-280 mesh sieve according to
The feed liquid weight of eliminating sargassum smell Spirulina powder and water and volume 1:10-1:30 carry out batch mixing, after material mix homogeneously, are placed in ultrasound wave
In processing meanss, control ultrasonic power for 100W-800W, ultrasonic time be 5min-35min after, obtain eliminating sargassum smell Spirulina powder pre-
Treatment fluid；
4）Described high-voltage pulse electric field technology auxiliary fiber element enzyme Controlled-enzymatic Hydrolysis process, is that cellulase is added to eliminating sargassum smell spiral shell
In spirulina powder treatment fluid, enzyme is 5%-7% than concentration of substrate, and aids in, with high-pressure pulse electric circulating treatment procedure, using distilled water
With ethanol purge constant flow pump and high-pressure pulse electric feed back pipe 2-5 time, at ambient temperature, by the process of eliminating sargassum smell Spirulina powder
Liquid pumps into high-pressure pulse electric system with the flow velocity of 1.6-4.8 mL/min, opens high-voltage pulse electric field switch, adjusts electric field
Field intensity 5-25 kV/cm and pulse frequency 800-2400HZ, after each run 2-3 min, collect treatment fluid, add cellulase
Treatment fluid pH value 6-10, circular treatment 6-8 time is kept to obtain the process of eliminating sargassum smell Spirulina powder after final regulation treatment fluid pH value 8-11
Liquid；
5）Described concentration process, is that eliminating sargassum smell Spirulina powder treatment fluid is carried out process concentrated in vacuo, and vacuum is 0.065Mpa-
0.095Mpa, temperature are 40 DEG C -85 DEG C, concentrate eliminating sargassum smell Spirulina powder treatment fluid, obtain eliminating sargassum smell Spirulina powder concentrated solution；
6）Described vacuum freeze-drying dry run, is that eliminating sargassum smell Spirulina powder concentrated solution is refrigerated to subzero 25-50 DEG C, then takes out true
Sky heats to obtain eliminating sargassum smell spirulina powder, and wherein evacuation makes pressure be maintained at 100-120Pa, and temperature is maintained at 25-50 DEG C；
7）Described granulation, tableting processes, are by eliminating sargassum smell spirulina powder, cross the granulation of 50-80 mesh sieves by oscillating granulator,
Eliminating sargassum smell spirulina powder after by granulation, tabletting under conditions of temperature 20-22 DEG C, humidity 10-30%, selection pressure is
3000N-4000N, obtains radioprotective eliminating sargassum smell spiral algae sheet.
4. according to the preparation method of the radioprotective eliminating sargassum smell spiral algae sheet described in claim 3, it is characterised in that described is concentrated
Journey, 1/2-1/6 of the eliminating sargassum smell Spirulina powder treatment fluid after concentration for original volume.
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| CN110437309A (en) * | 2019-09-04 | 2019-11-12 | 安徽国肽生物科技有限公司 | A kind of algae protein peptides and preparation method thereof inhibiting function with DPP-IV |
| CN111000022A (en) * | 2019-12-03 | 2020-04-14 | 襄阳蒙肽生物有限公司 | Preparation method of spirulina polypeptide powder and spirulina polypeptide tablet |
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