CN106490066A - A kind of microbial environment protecting insecticide and preparation method thereof - Google Patents
A kind of microbial environment protecting insecticide and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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Abstract
The invention belongs to technical field of pesticide, and in particular to a kind of microbial environment protecting insecticide and preparation method thereof, the microbial environment protecting insecticide include that the raw material of following parts by weight is made：8 13 parts of bacillus subtilis powder end, 6 10 parts of lactic acid bacteria powder, 5 10 parts of trichoderma powder, 8 13 parts of Herba et Flos Pyrethri Cinerariifolii, 38 parts of Rhizoma Osmundae, 7 12 parts of Rhizoma Polygoni Cuspidati, 36 parts of Celastrus angulatus, 58 parts of Cortex erythrinae, 100 150 parts of 7 11 parts of Radix Coicis, 10 20 parts of emulsifying agent, 0 10 parts of synergist and water.Microbial environment protecting insecticide environmental protection of the present invention, abnormal smells from the patient are refrigerant, benumb insect nervous system, and significantly, killing rate also helps plant growth up to 93.3% to insecticidal effect.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of microbial environment protecting insecticide and preparation method thereof.
Background technology
Tradition is applied to veterinary antibiotics, the various pesticide of grain are respectively provided with stronger toxicity, and after use, its toxicity is different
Degree it is absorbed into inside veterinary antibiotics, grain, can not be fully erased before eating, serious harm human health, Er Qienong
Medicine is remained and causes certain pollution to environment.In recent years, with the improvement of people's living standards, the extract of plant or plant is added
Work makes insecticide, and which possesses low toxicity, degradable, and Environmental compatibility is good, and to killing natural enemy, insect is not likely to produce drug-fast excellent
Point, is increasingly welcome by people.
Chinese patent CN104170871A discloses a kind of agricultural micro organism Pesticide with Chinese medicinal material, by following parts by weight
Composition is constituted：Lactic acid bacteria powder 5-13 parts, nitrobacteria powder 1-3 part, rhizobium powder 5-6 part, Zymomonas mobiliss powder 8-
10 parts, candidiasises powder 9-13 parts, Rhodococcus fascians powder 4-13 parts, Flos Chrysanthemi 7-13 parts, Radix Hemsleyae Macrospermae 9-13 parts, Radix Dichroae 1-7
Part, Periostracum Serpentiss 3-8 parts, Polyporus 3-7 parts, Fructus Cannabiss 5-9 parts, Radix Chloranthi Serrati 5-8 parts, chrysanthemum height 5-12 part, caffeine 1-6
Part, synergist 1-10 parts, Nicotiana tabacum L. 20-30 parts, Radix Phytolaccae 10-15 parts, Aconitum carmichjaelii Debx. 10-20 parts, 100 parts of water.But, this is special
Sharp microorganism and raw material of Chinese medicine synergy are poor, not degradable, and raw material type is various, high cost.
Chinese patent 105410070A discloses a kind of green pesticide containing microorganism, in parts by weight,
Including the following raw material：Photosynthetic bacteria liquid 3-5 parts, Semen Ignatii 8-12 parts, organosilicon 1-3 parts, Beauveria tenella 8-12
Part, Herba Menthae 10-20 parts, cabo 6-10 parts, Paecilomyces varioti 4-6 parts, galic bolt 20-30 parts, Bulbus Allii Cepae 8-12 parts, seat shell spore
Bacterium 3-5 parts, Radix Tripterygii Wilfordii 8-10 parts, 200 parts of water.But, the patent insecticide insecticidal effect is not obvious.
Content of the invention
For overcoming drawbacks described above, it is an object of the invention to provide a kind of microbial environment protecting insecticide and preparation method thereof.
For achieving the above object, the present invention is adopted the following technical scheme that：
A kind of microbial environment protecting insecticide, the raw material including following parts by weight are made：
Bacillus subtilis powder end（Bacillus subtilis）8-13 parts, lactic acid bacteria powder（lactic acid
bacteria）6-10 parts, trichoderma powder（Trichoderma）5-10 parts, Herba et Flos Pyrethri Cinerariifolii 8-13 parts, Rhizoma Osmundae 3-8 part, Rhizoma Polygoni Cuspidati
7-12 parts, Celastrus angulatus 3-6 parts, Cortex erythrinae 5-8 parts, Radix Coicis 7-11 parts, emulsifying agent 10-20 parts, synergist 0-10 parts and water
100-150 parts.
Preferably, bacillus subtilis powder end living bacteria count >=3 × 108Cfu/g, the effective viable bacteria of lactic acid bacteria powder
Number >=5 × 106Cfu/g, trichoderma powder living bacteria count >=1 × 108cfu/g.
Preferably, Folium Artemisiae Argyi 7-12 parts are also included.
Preferably, the emulsifying agent is agriculture breast 600 and/or agriculture breast 2201.
Preferably, the synergist is at least one of organosilicon, Butacide, alkyl carbonate and laurocapram.
A kind of above-mentioned microbial environment protecting insecticide preparation method, comprises the following steps：
（1）The various raw material of Chinese medicine of the parts by weight are weighed, be dry, pulverize, mix homogeneously；
（2）By step（1）The water of 8-12 times of weight is added in compound, soaks 1-2h, be subsequently adding under the conditions of 40-60 DEG C
The cellulase of 0.3-1.0% carries out enzymolysis 1-2h, Sonication assisted treatment 20-40min, filters, and collects extracting solution；
（3）By step（2）Middle gained extracting solution concentration removes solvent, is spray-dried, obtains Chinese medicine extract；
（4）By step（3）Chinese medicine extract and microorganism raw material are sequentially added in water, then sequentially add emulsifying agent, synergist,
Stir, obtain final product microbial environment protecting insecticide.
Preferably, step（2）The enzymolysis pH is 4.0-5.5.
Preferably, step（2）Ultrasonic frequency 15-25kHz.
Positive beneficial effect of the present invention：
1. the present invention is shared using microorganism Bacillus subtilis, lactic acid bacteria, trichoderma and raw material of Chinese medicine, is contained in microorganism
Enzyme, can convert the ingredient of Chinese medicine, make the release completely of Effective Component of Chinese Medicine, and microorganism is to person poultry safety, right
Environmental nonpollution, be not likely to produce Drug resistance, high-efficiency broad spectrum, promote plant growth.Herba et Flos Pyrethri Cinerariifolii can make respiratory of insect maincenter fiber crops
Numbness and dead；Rhizoma Osmundae is bitter, is slightly cold, heat-clearing and toxic substances removing, and parasite killing stops blooding；Rhizoma Polygoni Cuspidati slight bitter, is slightly cold, and with Huoxue San " expectorant, wind-dispelling removing toxic substances, disappears
Scorching pain relieving, remuval of damp and hot jaundice, the effects such as control chronic tracheitiss, reduction blood fat, can pesticide processed, to preventing snout moths larva, aphid etc. effectively；
Celastrus angulatus has stomach toxicity, anesthetic action containing Celastrus angulatus to insect；Cortex erythrinae bitter in the mouth, pungent, mild-natured, wind-damp dispelling, the meridian dredging, kill
Worm；Radix Coicis are sweet, light, are slightly cold, heat clearing away, dampness removing, parasite killing, and each raw material type of the invention is simple, with low cost, various raw material functions
Complementation, gained insecticide environmental protection, abnormal smells from the patient are refrigerant, benumb insect nervous system, and significantly, killing rate is reachable for insecticidal effect
93.3%, also help plant growth.
2. microorganism Bacillus subtilis of the present invention, Bacillus thuringiensis, trichoderma vigor are high, enhance insecticide antibacterial
Effect.Folium Artemisiae Argyi fragrance is strong, inhibited to polypide gastrointestinal tract, enhances the anthelminthic effect of insecticide.Emulsifying of the present invention
Agent is agriculture breast 600 and/or agriculture breast 2201, makes various water-soluble materials and Oil soluble materials dissolve each other completely, and the synergist is have
At least one of machine silicon, Butacide, alkyl carbonate and laurocapram, with low surface tension, good plate-out ability, infiltration
Property and emulsifying dispersivity, effectively suppress or reduction insect be to the anti-poisoning effect of the removing toxic substances of pesticide activity.Parasite killing prepared by the present invention
Agent is easily flowed, not bleed, not stratified, does not lump, and can stably preserve 1-2.
3. each raw material of Chinese medicine of invention is root, stem and leaf class, biological enzymolysis and ultrasound wave associated methods to effectively into
Divide and extract completely, effective component extraction rate is high, with low cost.
Specific embodiment
With reference to some specific embodiments, the present invention is further described.
Embodiment 1
A kind of microbial environment protecting insecticide, the raw material including following parts by weight are made：
8 parts of bacillus subtilis powder end, 6 parts of lactic acid bacteria powder, 6 parts of trichoderma powder, 10 parts of Herba et Flos Pyrethri Cinerariifolii, 5 parts of Rhizoma Osmundae, tiger
100 parts of 7 parts of cane, 3 parts of Celastrus angulatus, 5 parts of Cortex erythrinae, 8 parts of Radix Coicis, 10 parts of emulsifying agent, 2 parts of synergist and water.
Bacillus subtilis powder end living bacteria count >=3 × 108Cfu/g, lactic acid bacteria powder living bacteria count >=5 ×
106Cfu/g, trichoderma powder living bacteria count >=1 × 108cfu/g.
The emulsifying agent is agriculture breast 600.
The synergist is Butacide.
Above-mentioned microbial environment protecting insecticide preparation method, comprises the following steps：
（1）The various raw material of Chinese medicine of the parts by weight are weighed, be dry, pulverize, mix homogeneously；
（2）By step（1）The water of 8 times of weight is added in compound, soaks 1h, be subsequently adding 0.5% fibre under the conditions of 40 DEG C
The plain enzyme of dimension carries out enzymolysis 1.5h, and the enzymolysis pH is 4.0, Sonication assisted treatment 30min, filters, collects extracting solution；Described super
Frequency of sound wave 15kHz；
（3）By step（2）Middle gained extracting solution concentration removes solvent, is spray-dried, obtains Chinese medicine extract；
（4）By step（3）Chinese medicine extract and microorganism raw material are sequentially added in water, then sequentially add emulsifying agent, synergist,
Stir, obtain final product.
Embodiment 2
A kind of microbial environment protecting insecticide, the raw material including following parts by weight are made：
8 parts of bacillus subtilis powder end, 6 parts of lactic acid bacteria powder, 5 parts of trichoderma powder, 8 parts of Herba et Flos Pyrethri Cinerariifolii, 3 parts of Rhizoma Osmundae, tiger
100 parts of 7 parts of cane, 3 parts of Celastrus angulatus, 5 parts of Cortex erythrinae, 7 parts of Radix Coicis, 7 parts of Folium Artemisiae Argyi, 10 parts of emulsifying agent and water.
Bacillus subtilis powder end living bacteria count >=3 × 108Cfu/g, lactic acid bacteria powder living bacteria count >=5 ×
106Cfu/g, trichoderma powder living bacteria count >=1 × 108cfu/g.
The emulsifying agent is agriculture breast 600.
Above-mentioned microbial environment protecting insecticide preparation method, comprises the following steps：
（1）The various raw material of Chinese medicine of the parts by weight are weighed, be dry, pulverize, mix homogeneously；
（2）By step（1）The water of 10 times of weight is added in compound, soaks 1.5h, be subsequently adding 0.3% under the conditions of 50 DEG C
Cellulase carries out enzymolysis 2h, and the enzymolysis pH is 5.5, Sonication assisted treatment 20min, filters, collects extracting solution；Described super
Frequency of sound wave 20kHz；
（3）By step（2）Middle gained extracting solution concentration removes solvent, is spray-dried, obtains Chinese medicine extract；
（4）By step（3）Chinese medicine extract and microorganism raw material are sequentially added in water, then sequentially add emulsifying agent, and stirring is
Even, obtain final product.
Embodiment 3
A kind of microbial environment protecting insecticide, the raw material including following parts by weight are made：
9 parts of bacillus subtilis powder end, 7 parts of lactic acid bacteria powder, 6 parts of trichoderma powder, 9 parts of Herba et Flos Pyrethri Cinerariifolii, 4 parts of Rhizoma Osmundae, tiger
110 parts of 8 parts of cane, 3.5 parts of Celastrus angulatus, 6 parts of Cortex erythrinae, 8 parts of Radix Coicis, 8 parts of Folium Artemisiae Argyi, 12 parts of emulsifying agent, 1 part of synergist and water.
Bacillus subtilis powder end living bacteria count >=3 × 108Cfu/g, lactic acid bacteria powder living bacteria count >=5 ×
106Cfu/g, trichoderma powder living bacteria count >=1 × 108cfu/g.
The emulsifying agent is agriculture breast 2201.
The synergist is organosilicon.
Above-mentioned microbial environment protecting insecticide preparation method, comprises the following steps：
（1）The various raw material of Chinese medicine of the parts by weight are weighed, be dry, pulverize, mix homogeneously；
（2）By step（1）The water of 12 times of weight is added in compound, soaks 2h, be subsequently adding 0.5% fibre under the conditions of 60 DEG C
The plain enzyme of dimension carries out enzymolysis 1h, and the enzymolysis pH is 4.5, Sonication assisted treatment 40min, filters, collects extracting solution；The ultrasound
Wave frequency 25kHz；
（3）By step（2）Middle gained extracting solution concentration removes solvent, is spray-dried, obtains Chinese medicine extract；
（4）By step（3）Chinese medicine extract and microorganism raw material are sequentially added in water, then sequentially add emulsifying agent, synergist,
Stir, obtain final product.
Embodiment 4
A kind of microbial environment protecting insecticide, the raw material including following parts by weight are made：
10 parts of bacillus subtilis powder end, 8 parts of lactic acid bacteria powder, 7 parts of trichoderma powder, 10 parts of Herba et Flos Pyrethri Cinerariifolii, 5 parts of Rhizoma Osmundae,
9 parts of Rhizoma Polygoni Cuspidati, 4 parts of Celastrus angulatus, 6.5 parts of Cortex erythrinae, 9 parts of Radix Coicis, 9 parts of Folium Artemisiae Argyi, 15 parts of emulsifying agent, 5 parts of synergist and water 120
Part.
Bacillus subtilis powder end living bacteria count >=3 × 108Cfu/g, lactic acid bacteria powder living bacteria count >=5 ×
106Cfu/g, trichoderma powder living bacteria count >=1 × 108cfu/g.
The emulsifying agent is agriculture breast 600 and agriculture breast 2201, and both weight ratios are 1：1.
The synergist is Butacide.
Above-mentioned microbial environment protecting insecticide preparation method, comprises the following steps：
（1）The various raw material of Chinese medicine of the parts by weight are weighed, be dry, pulverize, mix homogeneously；
（2）By step（1）The water of 10 times of weight is added in compound, soaks 1.5h, be subsequently adding 0.8% under the conditions of 50 DEG C
Cellulase carries out enzymolysis 1.5h, and the enzymolysis pH is 5.0, Sonication assisted treatment 30min, filters, collects extracting solution；Described
Ultrasonic frequency 20kHz；
（3）By step（2）Middle gained extracting solution concentration removes solvent, is spray-dried, obtains Chinese medicine extract；
（4）By step（3）Chinese medicine extract and microorganism raw material are sequentially added in water, then sequentially add emulsifying agent, synergist,
Stir, obtain final product.
Embodiment 5
A kind of microbial environment protecting insecticide, the raw material including following parts by weight are made：
10 parts of bacillus subtilis powder end, 8 parts of lactic acid bacteria powder, 7 parts of trichoderma powder, Paecilomyces lilacinus powder
（Paecilomyces lilacinus(Thom.)Samson）5 parts, 10 parts of Herba et Flos Pyrethri Cinerariifolii, 5 parts of Rhizoma Osmundae, 9 parts of Rhizoma Polygoni Cuspidati, bitter skin
120 parts of 4 parts of rattan, 6.5 parts of Cortex erythrinae, 9 parts of Radix Coicis, 9 parts of Folium Artemisiae Argyi, 15 parts of emulsifying agent, 5 parts of synergist and water.
Microorganism Paecilomyces lilacinus are added in the present embodiment, and Paecilomyces lilacinus bacterium is novel pure microorganism work spore system
Agent, with efficient, wide spectrum, long-acting, safe and pollution-free, noresidue feature, can obvious stimulation plant growth, close with other raw materials
With, can more preferably prevent and treat soil pests and infect, enhancing insecticide drug effect.
Bacillus subtilis powder end living bacteria count >=3 × 108Cfu/g, lactic acid bacteria powder living bacteria count >=5 ×
106Cfu/g, trichoderma powder living bacteria count >=1 × 108Cfu/g, Paecilomyces lilacinus powder living bacteria count >=3 ×
106cfu/mg.
The emulsifying agent is agriculture breast 600 and agriculture breast 2201, and both weight ratios are 1：1.
The synergist is Butacide.
Above-mentioned microbial environment protecting insecticide preparation method is shown in embodiment 4.
Embodiment 6
A kind of microbial environment protecting insecticide, the raw material including following parts by weight are made：
11 parts of bacillus subtilis powder end, 9 parts of lactic acid bacteria powder, 10 parts of trichoderma powder, 11 parts of Herba et Flos Pyrethri Cinerariifolii, 6 parts of Rhizoma Osmundae,
10 parts of Rhizoma Polygoni Cuspidati, 4.5 parts of Celastrus angulatus, 7 parts of Cortex erythrinae, 9.5 parts of Radix Coicis, 10 parts of Folium Artemisiae Argyi, 16 parts of emulsifying agent, 6 parts of synergist and water
130 parts.
Bacillus subtilis powder end living bacteria count >=3 × 108Cfu/g, lactic acid bacteria powder living bacteria count >=5 ×
106Cfu/g, trichoderma powder living bacteria count >=1 × 108cfu/g.
The emulsifying agent is agriculture breast 600.
The synergist is alkyl carbonate.
Above-mentioned microbial environment protecting insecticide preparation method, comprises the following steps：
（1）The various raw material of Chinese medicine of the parts by weight are weighed, be dry, pulverize, mix homogeneously；
（2）By step（1）The water of 9 times of weight is added in compound, soaks 1h, be subsequently adding 1.0% fibre under the conditions of 50 DEG C
The plain enzyme of dimension carries out enzymolysis 1.5h, and the enzymolysis pH is 5.0, Sonication assisted treatment 20min, filters, collects extracting solution；Described super
Frequency of sound wave 15kHz；
（3）By step（2）Middle gained extracting solution concentration removes solvent, is spray-dried, obtains Chinese medicine extract；
（4）By step（3）Chinese medicine extract and microorganism raw material are sequentially added in water, then sequentially add emulsifying agent, synergist,
Stir, obtain final product.
Embodiment 7
A kind of microbial environment protecting insecticide, the raw material including following parts by weight are made：
12 parts of bacillus subtilis powder end, 10 parts of lactic acid bacteria powder, 9 parts of trichoderma powder, 12 parts of Herba et Flos Pyrethri Cinerariifolii, 7 parts of Rhizoma Osmundae,
11 parts of Rhizoma Polygoni Cuspidati, 5 parts of Celastrus angulatus, 7.5 parts of Cortex erythrinae, 10 parts of Radix Coicis, 11 parts of Folium Artemisiae Argyi, 18 parts of emulsifying agent, 8 parts of synergist and water
140 parts.
Bacillus subtilis powder end living bacteria count >=3 × 108Cfu/g, lactic acid bacteria powder living bacteria count >=5 ×
106Cfu/g, trichoderma powder living bacteria count >=1 × 108cfu/g.
The emulsifying agent is agriculture breast 2201.
The synergist is laurocapram.
Above-mentioned microbial environment protecting insecticide preparation method, comprises the following steps：
（1）The various raw material of Chinese medicine of the parts by weight are weighed, be dry, pulverize, mix homogeneously；
（2）By step（1）The water of 10 times of weight is added in compound, soaks 1h, be subsequently adding 1.0% fibre under the conditions of 60 DEG C
The plain enzyme of dimension carries out enzymolysis 1h, and the enzymolysis pH is 5.5, Sonication assisted treatment 30min, filters, collects extracting solution；The ultrasound
Wave frequency 25kHz；
（3）By step（2）Middle gained extracting solution concentration removes solvent, is spray-dried, obtains Chinese medicine extract；
（4）By step（3）Chinese medicine extract and microorganism raw material are sequentially added in water, then sequentially add emulsifying agent, synergist,
Stir, obtain final product.
Embodiment 8
A kind of microbial environment protecting insecticide, the raw material including following parts by weight are made：
13 parts of bacillus subtilis powder end, 10 parts of lactic acid bacteria powder, 10 parts of trichoderma powder, 13 parts of Herba et Flos Pyrethri Cinerariifolii, Rhizoma Osmundae 8
Part, 12 parts of Rhizoma Polygoni Cuspidati, 6 parts of Celastrus angulatus, 8 parts of Cortex erythrinae, 11 parts of Radix Coicis, 12 parts of Folium Artemisiae Argyi, 20 parts of emulsifying agent, 10 parts of synergist and water
150 parts.
Bacillus subtilis powder end living bacteria count >=3 × 108Cfu/g, lactic acid bacteria powder living bacteria count >=5 ×
106Cfu/g, trichoderma powder living bacteria count >=1 × 108cfu/g.
The emulsifying agent is agriculture breast 2201.
The synergist is Butacide and laurocapram, and both weight ratios are 1：1.
Above-mentioned microbial environment protecting insecticide preparation method, comprises the following steps：
（1）The various raw material of Chinese medicine of the parts by weight are weighed, be dry, pulverize, mix homogeneously；
（2）By step（1）The water of 8 times of weight is added in compound, soaks 2h, be subsequently adding 0.5% fibre under the conditions of 40 DEG C
The plain enzyme of dimension carries out enzymolysis 2h, and the enzymolysis pH is 4.5, Sonication assisted treatment 40min, filters, collects extracting solution；The ultrasound
Wave frequency 20kHz；
（3）By step（2）Middle gained extracting solution concentration removes solvent, is spray-dried, obtains Chinese medicine extract；
（4）By step（3）Chinese medicine extract and microorganism raw material are sequentially added in water, then sequentially add emulsifying agent, synergist,
Stir, obtain final product.
Comparative example 1
The present embodiment microbial environment protecting insecticide is substantially the same manner as Example 4, and difference is：
The microbial environment protecting insecticide includes that the raw material of following parts by weight is made：
10 parts of Herba et Flos Pyrethri Cinerariifolii, 5 parts of Rhizoma Osmundae, 9 parts of Rhizoma Polygoni Cuspidati, 4 parts of Celastrus angulatus, 6.5 parts of Cortex erythrinae, 9 parts of Radix Coicis, 9 parts of Folium Artemisiae Argyi, breast
120 parts of 15 parts of agent, 5 parts of synergist and water.
Comparative example 2
The present embodiment microbial environment protecting insecticide is substantially the same manner as Example 4, and difference is：
The microbial environment protecting insecticide includes that the raw material of following parts by weight is made：
10 parts of bacillus subtilis powder end, 8 parts of lactic acid bacteria powder, 7 parts of trichoderma powder, 5 parts of Rhizoma Osmundae, 9 parts of Rhizoma Polygoni Cuspidati, Cortex erythrinae
6.5 parts, 9 parts of Radix Coicis, 9 parts of Folium Artemisiae Argyi, 15 parts of emulsifying agent, 120 parts of 5 parts of synergist and water.
Comparative example 3
The present embodiment microbial environment protecting insecticide is substantially the same manner as Example 4, and difference is：
The microbial environment protecting insecticide includes that the raw material of following parts by weight is made：
10 parts of bacillus subtilis powder end, 8 parts of lactic acid bacteria powder, 7 parts of trichoderma powder, 10 parts of Herba et Flos Pyrethri Cinerariifolii, Celastrus angulatus 4
120 parts of part, 6.5 parts of Cortex erythrinae, 9 parts of Radix Coicis, 9 parts of Folium Artemisiae Argyi, 15 parts of emulsifying agent, 5 parts of synergist and water.
Comparative example 4
The present embodiment microbial environment protecting insecticide is substantially the same manner as Example 4, and difference is：The microbial environment protecting parasite killing
Agent includes that the raw material of following parts by weight is made：
10 parts of bacillus subtilis powder end, 8 parts of lactic acid bacteria powder, 7 parts of trichoderma powder, 10 parts of Herba et Flos Pyrethri Cinerariifolii, 5 parts of Rhizoma Osmundae,
120 parts of 9 parts of Rhizoma Polygoni Cuspidati, 4 parts of Celastrus angulatus, 9 parts of Folium Artemisiae Argyi, 15 parts of emulsifying agent, 5 parts of synergist and water.
Application test
Embodiment of the present invention 1-8 and matched group 1-3 microbial environment protecting insecticides are determined to wireworm using the mixed medicine method of soil
Insecticidal effect, collection sandy loam cross 40 mesh sieves, disinfect 4h, weigh 1kg soil, sequentially add 1mg medicinal liquids under the conditions of 120 DEG C
And suitable quantity of water, it is 18% to make soil moisture content, stirs, and loading has accessed the glass of 1 wireworm and supported in scolite, is put into few
The wheat seed of amount just germination often processes test worm 60, is repeated 3 times, and sets distilled water as blank as food（CK）,
24 ± 2 DEG C are placed in, are cultivated in the greenhouse of humidity 60 ± 5%, after 72h, observe and record the death condition of test worm, polypide is substantially received
It is then dead that contracting or the test worm that touches normally can not be creeped, and measurement result is shown in Table 1.
1 embodiment of the present invention 1-8 microbial environment protecting insecticide insecticidal effect testing result of table
As shown in Table 1, up to more than 80%, embodiment 5 is due to more for embodiment of the present invention 1-8 microbial environment protecting insecticide killing rate
Add plus microorganism raw material Paecilomyces lilacinus, killing rate lacks microorganism raw material up to 93.3% in 1 raw material of comparative example,
Comparative example 2 lacks Herba et Flos Pyrethri Cinerariifolii and Celastrus angulatus, and comparative example 3 lacks Rhizoma Osmundae and Rhizoma Polygoni Cuspidati, and comparative example 4 lacks sea
Cortex paulowniae and Radix Coicis, killing rate then substantially reduce, and illustrate that each raw material collaboration of embodiment of the present invention 1-8 microbial environment protecting insecticide is made
With notable.
Claims (8)
1. a kind of microbial environment protecting insecticide, it is characterised in that the raw material including following parts by weight is made：
Bacillus subtilis powder end（Bacillus subtilis）8-13 parts, lactic acid bacteria powder（lactic acid
bacteria）6-10 parts, trichoderma powder（Trichoderma）5-10 parts, Herba et Flos Pyrethri Cinerariifolii 8-13 parts, Rhizoma Osmundae 3-8 part, Rhizoma Polygoni Cuspidati
7-12 parts, Celastrus angulatus 3-6 parts, Cortex erythrinae 5-8 parts, Radix Coicis 7-11 parts, emulsifying agent 10-20 parts, synergist 0-10 parts and water
100-150 parts.
2. microbial environment protecting insecticide according to claim 1, it is characterised in that the bacillus subtilis powder end is effectively
Viable count >=3 × 108Cfu/g, lactic acid bacteria powder living bacteria count >=5 × 106Cfu/g, trichoderma powder living bacteria count >=1
×108cfu/g.
3. microbial environment protecting insecticide according to claim 1, it is characterised in that also include Folium Artemisiae Argyi 7-12 parts.
4. microbial environment protecting insecticide according to claim 3, it is characterised in that the emulsifying agent be agriculture breast 600 and/or
Agriculture breast 2201.
5. microbial environment protecting insecticide according to claim 3, it is characterised in that the synergist is organosilicon, potentiation
At least one of ether, alkyl carbonate and laurocapram.
6. the microbial environment protecting insecticide preparation method described in a kind of any one of claim 1-5, it is characterised in that including following
Step：
（1）The various raw material of Chinese medicine of the parts by weight are weighed, be dry, pulverize, mix homogeneously；
（2）By step（1）The water of 8-12 times of weight is added in compound, soaks 1-2h, be subsequently adding under the conditions of 40-60 DEG C
The cellulase of 0.3-1.0% carries out enzymolysis 1-2h, Sonication assisted treatment 20-40min, filters, and collects extracting solution；
（3）By step（2）Middle gained extracting solution concentration removes solvent, is spray-dried, obtains Chinese medicine extract；
（4）By step（3）Chinese medicine extract and microorganism raw material are sequentially added in water, then sequentially add emulsifying agent, synergist,
Stir, obtain final product microbial environment protecting insecticide.
7. microbial environment protecting insecticide preparation method according to claim 6, it is characterised in that step（2）The enzymolysis
PH is 4.0-5.5.
8. microbial environment protecting insecticide preparation method according to claim 6, it is characterised in that step（2）The ultrasound
Wave frequency 15-25kHz.
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Application publication date: 20170315 |