CN106489554A - A kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis - Google Patents

A kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis Download PDF

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Publication number
CN106489554A
CN106489554A CN201611214442.1A CN201611214442A CN106489554A CN 106489554 A CN106489554 A CN 106489554A CN 201611214442 A CN201611214442 A CN 201611214442A CN 106489554 A CN106489554 A CN 106489554A
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root
cuttage
parent
old
parent root
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蒋泽平
汪有良
蒋春
邢玮
黄利斌
马骧
朱朝芹
王玉刚
戴惠忠
吴瑞石
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Jiangsu Forestry Academy
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Jiangsu Forestry Academy
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present invention relates to a kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis, mainly includes the following steps that:(1), parent root is chosen:Take kind of a root after 10 November fallen leaves, selection rugosity is the root bar of 2 4mm, and root bar is cut into the segment of 4 8cm length, as parent root;(2), parent root storage:Parent root is carried out husky Tibetan, first 10 20 days, thickness is placed in for storing in 5cm, the sandy soil that relative humidity is 50%, after be covered with the dry sand of 2 3cm, 57 days before parent root cuttage, carry out spraying irrigation to sandy soil upper strata;(3), parent root is processed:Carry out ultraviolet after parent root is taken out from sandy soil immediately and irradiate 2 3min, two ends are trimmed to horse ear shape;(4), cuttage:The parent root otch that has pruned is put down down and is embedded in substrate, root depth is buried for 0.5 1cm;(5), manage after cuttage.The present invention is significant for the selection-breeding of yellow wingceltis new varieties, and cuttage survival rate is high.

Description

A kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis
Technical field
A kind of the present invention relates to raising technology of yellow wingceltis, more particularly to root segment cutting propagation side of century-old raw old-age group yellow wingceltis Method.
Background technology
Yellow wingceltis (scientific name:Dalbergia hupeana Hance), another name:Do not know the spring, hope water wingceltis, Tan Shu, sandalwood, Symplocoris Paniculatae; Papilionaceae, Dalbergia magaphanerophytes.Bark lead, winglike compound leaf, panicle, corolla lavender or white, pod are long Circular or wealthy ligule, the flowering fruit bearing stage 5-10 months.
Not tight to growing environmental requirement, it is Landscape Application high-quality seeds;Wood quality structure is fine and closely woven, is high-quality negative G and drawing The strong apparatus of power and equipment making material.Root bark is pharmaceutically acceptable, the effect of with heat-clearing and toxic substances removing, hemostasia and detumescence.In being distributed widely in State various places.
The asexual propagation of yellow wingceltis can adopt the method such as branch cutting and grafting, but as inventor is in election effects examination Test middle discovery, aged yellow wingceltis branch cutting or engrafting method, survival rate is low, more defective be, this asexual propagation into Seedling after work, poor growth, vitality be not strong, should not carry out the Clone Selection And Reproduction research of next step.And grind in yellow wingceltis selection-breeding During the germ plasm resource that studies carefully is collected, the age of tree of most resource Superior line has been above 100 years, adopts in Clone Selection And Reproduction research With Cutting root reproduction, as root segment phasic development is young, cytoactive is strong, and not only survival rate is high for cuttage, and the childrenization that raises up seed, Growth is normal, is highly suitable for Clone Selection And Reproduction.
Content of the invention
In order to solve above-mentioned technical problem, there is provided a kind of cuttage survival rate is high, the aged yellow wingceltis root segment that juvenile traits are stable Cuttage and seedling culture method, the present invention provide technical scheme below:
A kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis, comprises the following steps:
(1), parent root is chosen:Take kind of a root after falling leaves the 10-11 months, rugosity is selected for the root bar of 2-4mm, and root bar is cut into The segment of 4-8cm length, as parent root;
In conventional root insert method, select the root bar that rugosity is 15-20mm as parent root, root bar diameter is thick, and then which includes more Nutrient substance and Endogenous Hormone Contents in Vitro high, be conducive to the growth of rudiment, but rugosity height mean the range of choice of root bar Diminish, qualified bar quantity tails off, and root bar is more thick is more unfavorable for which is taken root on the contrary, therefore, the present invention is breakthrough From less bar of rugosity as parent root, on the premise of its rooting rate is ensured, root bar rugosity is made up by subsequent treatment little The adverse effect brought by sprout growth.
(2), parent root storage:Parent root is carried out husky Tibetan, and first 10-20 days, it was 50% thickness to be placed in for 5cm, relative humidity Store in sandy soil, after be covered with the dry sand of 2-3cm, 5-7 days before parent root cuttage, carry out spraying irrigation to sandy soil upper strata, adjust humidity to 60%-70%;
Conventional parent root storage prevents root for remaining a constant humidity in the case where ensureing not do sth. in advance rudiment Section dehydration, differentiation that is of the invention then having carried out humidity to the parent root of Sha Zangzhong are controlled, and can keep cutting by humidity regulation Vigor, and the content of plant endogenous hormones can be improved to a certain extent.
(3), parent root is processed:The 2-3 months, carry out ultraviolet irradiation 2-3min immediately, irradiate agent after parent root being taken out from sandy soil Measure as 350-450mW/cm2, then two ends are trimmed to after horse ear shape the mixed solution for dipping washing water of rice and garlic extraneous liquid;
Tests prove that, at short notice, be conducive to improving aged yellow wingceltis cuttage using the ultraviolet irradiation of optimal dose Survival rate, parent root two ends are trimmed to horse ear shape is prepared for the plug flat of next step, be conducive to increasing parent root with The contact area of parent root and substrate after the contact area of washing water of rice and cuttage.Washing water of rice is dipped before cuttage can promote tangent plane The healing of wound, accelerates the speed of growth of root system.
(4), cuttage:The parent root otch that has pruned is put down down and is embedded in substrate, root depth is buried for 0.5-1cm;Test card Bright for aged yellow wingceltis, it is higher that the plug flat of root segment compares the perpendicular motility rate that is inserted into, and earthing is unsuitable blocked up, and earthing is blocked up to cutting seedling Take root unfavorable.
(5), manage after cuttage:It is constant temperature and humidity condition 40d to keep cutting propagation room, and thermostat temperature is 18-20 DEG C, constant humidity Humidity is 50%-60%.
Conventional cutting propagation room frequently with 25 DEG C or so of temperature, the selection of seeds and parent root for the present invention and Speech, if still maintain 25 DEG C of nursery temperature, it will the development for causing sprout growth too fast and being unfavorable for root.
Further, in step (1) parent root rugosity be 3mm, length be 5cm, this specification parent root application the present invention side Survival rate highest during method.
Further, the preparation method of the garlic extraneous liquid in step (3) is that the Bulbus Allii taken after crushing is placed in microwave In extractor, and ethanol that quality is 15-20 times of Bulbus Allii quality is added as extractant, in 85-95 DEG C, microwave output power For microwave extraction is carried out under conditions of 300W, filter after extracting 3-5min, obtain garlic extraneous liquid, add the master of garlic extraneous liquid Syllabus goes bad for preventing from dipping the notch after washing water of rice.
Further, step (4) mesostroma is made up of the following component in terms of mass fraction:Yellow soil 50-60 parts, peat Native 30-40 parts, coconut palm chaff 20-30 part, fermentation Glycine max (L.) Merr. 10-20 parts.Fermentation Glycine max (L.) Merr. is conducive to the shape that parent root incision wound is organized Into and the content of organic matter is abundant, may advantageously facilitate the accumulative of nutrient substance and endogenous hormones in parent root.
Further, step (4) mesostroma is made up of the following component in terms of mass fraction:55 parts of yellow soil, peat soil 35 parts, 25 parts of coconut palm chaff, fermentation 15 parts of Glycine max (L.) Merr..
The beneficial effects of the present invention is:
(1), the breakthrough less bar of selection rugosity of the present invention be used as parent root, on the premise of its rooting rate is ensured, The little adverse effect brought by sprout growth of root bar rugosity is made up by subsequent treatment, while so that the range of choice of parent root becomes Greatly.
(2) humid control of differentiation is adopted when, parent root sand is hidden, and can keep the vigor of parent root.
(3), before cuttage, parent root, using ultraviolet and rice washing water process, can promote the healing of tangent plane wound, accelerate root system The speed of growth.
(4), the cutting medium of improvement can significantly improve the content of endogenous hormones in parent root.
(5), the cutting seedling that is cultivated using the method for the present invention is highly suitable for the Clone Selection And Reproduction of yellow wingceltis.
Specific embodiment
Embodiment 1,
A kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis, comprises the following steps:
(1), parent root is chosen:Take kind of a root after falling leaves the 10-11 months, rugosity is selected for the root bar of 3mm, and root bar is cut into The segment of 5cm length, as parent root;
(2), parent root storage:Carry out husky Tibetan, first 15 days, being placed in thickness for 5cm, the sandy soil that relative humidity is 50% by parent root Middle storage, after be covered with the dry sand of 3cm, 7 days before parent root cuttage, carry out spraying irrigation to sandy soil upper strata, adjust humidity to 65%;
(3), parent root is processed:The 2-3 months, carry out ultraviolet irradiation 2.5min immediately, irradiate agent after parent root being taken out from sandy soil Measure as 400mW/cm2, then two ends are trimmed to after horse ear shape the mixed solution for dipping washing water of rice and garlic extraneous liquid;
(4), cuttage:The parent root otch that has pruned is put down down and is embedded in substrate, root depth is buried for 0.8cm.The substrate It is made up of the following component in terms of mass fraction:55 parts of yellow soil, 35 parts of peat soil, 25 parts of coconut palm chaff, 15 parts of Glycine max (L.) Merr. of fermentation.
(5), manage after cuttage:It is constant temperature and humidity condition 40d to keep cutting propagation room, and thermostat temperature is 18 DEG C, and constant humidity is wet Spend for 55%.
Embodiment 2,
A kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis, comprises the following steps:
(1), parent root is chosen:Take kind of a root after falling leaves the 10-11 months, rugosity is selected for the root bar of 4mm, and root bar is cut into The segment of 8cm length, as parent root;
(2), parent root storage:Carry out husky Tibetan, first 15 days, being placed in thickness for 5cm, the sandy soil that relative humidity is 50% by parent root Middle storage, after be covered with the dry sand of 3cm, 7 days before parent root cuttage, carry out spraying irrigation to sandy soil upper strata, adjust humidity to 65%;
(3), parent root is processed:The 2-3 months, carry out ultraviolet irradiation 2.5min immediately, irradiate agent after parent root being taken out from sandy soil Measure as 400mW/cm2, then two ends are trimmed to after horse ear shape the mixed solution for dipping washing water of rice and garlic extraneous liquid;
(4), cuttage:The parent root otch that has pruned is put down down and is embedded in substrate, root depth is buried for 0.8cm.The substrate It is made up of the following component in terms of mass fraction:55 parts of yellow soil, 35 parts of peat soil, 25 parts of coconut palm chaff, 15 parts of Glycine max (L.) Merr. of fermentation.
(5), manage after cuttage:It is constant temperature and humidity condition 40d to keep cutting propagation room, and thermostat temperature is 18 DEG C, and constant humidity is wet Spend for 55%.
Embodiment 3,
A kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis, comprises the following steps:
(1), parent root is chosen:Take kind of a root after falling leaves the 10-11 months, rugosity is selected for the root bar of 3mm, and root bar is cut into The segment of 5cm length, as parent root;
(2), parent root storage:Carry out husky Tibetan, first 15 days, being placed in thickness for 5cm, the sandy soil that relative humidity is 50% by parent root Middle storage, after be covered with the dry sand of 2cm, 5 days before parent root cuttage, carry out spraying irrigation to sandy soil upper strata, adjust humidity to 70%;
(3), parent root is processed:The 2-3 months, carry out ultraviolet irradiation 2min, exposure dose immediately after parent root being taken out from sandy soil For 350mW/cm2, then two ends are trimmed to after horse ear shape the mixed solution for dipping washing water of rice and garlic extraneous liquid;
(4), cuttage:The parent root otch that has pruned is put down down and is embedded in substrate, root depth is buried for 1cm;The substrate by Following component composition in terms of mass fraction::60 parts of yellow soil, 40 parts of peat soil, 30 parts of coconut palm chaff, 20 parts of Glycine max (L.) Merr. of fermentation.
(5), manage after cuttage:It is constant temperature and humidity condition 40d to keep cutting propagation room, and thermostat temperature is 20 DEG C, and constant humidity is wet Spend for 60%.
Comparative example 1,
A kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis, comprises the following steps:
(1), parent root is chosen:Take kind of a root after falling leaves the 10-11 months, rugosity is selected for the root bar of 15mm, and root bar is cut into The segment of 8cm length, as parent root;
(2), parent root storage:Parent root is carried out husky Tibetan, remains the humidity of sandy soil 50% or so;
(3), parent root is processed:Two ends are trimmed to horse ear shape;Both ends of the surface dip 2000 times of naphthalene acid solution;
(4), cuttage:The parent root otch that has pruned is put down down and is embedded in substrate, root depth is buried for 1cm;The substrate by Yellow soil, coconut palm chaff, rice chaff ash are with 3:1:1 ratio is mixed;
(5), manage after cuttage:It is constant temperature and humidity condition 40d to keep cutting propagation room, and thermostat temperature is 18 DEG C, and constant humidity is wet Spend for 50%.
Comparative example 2,
A kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis, comprises the following steps:
(1), parent root is chosen:Take kind of a root after falling leaves the 10-11 months, rugosity is selected for the root bar of 3mm, and root bar is cut into The segment of 5cm length, as parent root;
(2), parent root storage:Carry out husky Tibetan, first 15 days, being placed in thickness for 5cm, the sandy soil that relative humidity is 50% by parent root Middle storage, after be covered with the dry sand of 2.5cm, 7 days before parent root cuttage, carry out spraying irrigation to sandy soil upper strata, adjust humidity to 65%;
(3), parent root is processed:The 2-3 months, carry out ultraviolet irradiation 3min, exposure dose immediately after parent root being taken out from sandy soil For 400mW/cm2, then two ends are trimmed to horse ear shape;
(4), cuttage:The parent root otch that has pruned is erected down and is inserted in substrate, root depth is buried for 0.5cm;The substrate It is made up of the following component in terms of mass fraction:55 parts of yellow soil, 35 parts of peat soil, 25 parts of coconut palm chaff, 15 parts of Glycine max (L.) Merr. of fermentation.
(5), manage after cuttage:It is constant temperature and humidity condition 40d to keep cutting propagation room, and thermostat temperature is 25 DEG C, and constant humidity is wet Spend for 55%.
The method that above-mentioned 3 embodiments and 2 comparative examples is respectively adopted carries out root segment cutting propagation examination to century-old SHENGHUANG wingceltis Test, test data is as follows:
Analysis upper table understands that embodiment 1 is highly preferred embodiment of the present invention, and the survival rate of yellow wingceltis cutting seedling is 90% left side Right;Relative analyses embodiment 1 understands that with embodiment 2 it is slightly long more that embodiment 2 takes out can cutting seedling using the bigger parent root of rugosity Long, but survival rate reduction;Relative analyses embodiment 1 understood with embodiment 3, and substrate is affected with the survival rate for being compared to cutting seedling Larger;Relative analyses embodiment 1 understood with comparative example 1, and the improvement of the selection of rugosity, rice washing water process and substrate cooperates energy Survival rate, root system development speed and the sprout growth speed of root segment cuttage are enough greatly improved.
With above-mentioned foundation desirable embodiment of the present invention as enlightenment, by above-mentioned description, relevant staff is complete Various change and modification can be carried out in the range of without departing from this invention technological thought.This invention technical Scope is not limited to the content in description, it is necessary to determine its technical scope according to right.

Claims (5)

1. a kind of a century gives birth to the root segment cuttage and seedling culture method of old-age group yellow wingceltis, it is characterised in that comprise the following steps:
(1), parent root is chosen:Take kind of a root after falling leaves the 10-11 months, rugosity is selected for the root bar of 2-4mm, and root bar is cut into 4- The segment of 8cm length, as parent root;
(2), parent root storage:Carry out husky Tibetan, first 10-20 days, being placed in thickness for 5cm, the sandy soil that relative humidity is 50% by parent root Middle storage, after be covered with the dry sand of 2-3cm, 5-7 days before parent root cuttage, carry out spraying irrigation to sandy soil upper strata, adjust humidity to 60%- 70%;
(3), parent root is processed:The 2-3 months, carry out ultraviolet irradiation 2-3min, exposure dose is immediately after parent root being taken out from sandy soil 350-450mW/cm2, then two ends are trimmed to after horse ear shape the mixed solution for dipping washing water of rice and garlic extraneous liquid;
(4), cuttage:The parent root otch that has pruned is put down down and is embedded in substrate, root depth is buried for 0.5-1cm;(5), after cuttage Management:It is constant temperature and humidity condition 40d to keep cutting propagation room, and thermostat temperature is 18-20 DEG C, and constant humidity humidity is 50%-60%.
2. a kind of a century gives birth to the root segment cuttage and seedling culture method of old-age group yellow wingceltis as claimed in claim 1, it is characterised in that:Step (1) in parent root rugosity be 3mm, length be 5cm.
3. a kind of a century gives birth to the root segment cuttage and seedling culture method of old-age group yellow wingceltis as claimed in claim 1, it is characterised in that:Step (3) preparation method of the garlic extraneous liquid in is that the Bulbus Allii taken after crushing is placed in microwave extraction device, and adds the quality to be The ethanol of 15-20 times of Bulbus Allii quality is carried out under conditions of being 300W in 85-95 DEG C, microwave output power micro- as extractant Ripple is extracted, and filters, obtain garlic extraneous liquid after extracting 3-5min.
4. a kind of a century gives birth to the root segment cuttage and seedling culture method of old-age group yellow wingceltis as claimed in claim 1, it is characterised in that step (4) mesostroma is made up of the following component in terms of mass fraction:Yellow soil 50-60 parts, peat soil 30-40 parts, coconut palm chaff 20-30 Part, fermentation Glycine max (L.) Merr. 10-20 parts.
5. a kind of a century gives birth to the root segment cuttage and seedling culture method of old-age group yellow wingceltis as claimed in claim 4, it is characterised in that step (4) mesostroma is made up of the following component in terms of mass fraction:55 parts of yellow soil, 35 parts of peat soil, 25 parts of coconut palm chaff, fermentation bean curd 15 parts of slag.
CN201611214442.1A 2016-12-26 2016-12-26 A kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis Pending CN106489554A (en)

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CN107306729A (en) * 2017-09-20 2017-11-03 上海市园林科学规划研究院 Asimina triloba buries root propagation method
CN108055932A (en) * 2017-12-19 2018-05-22 安徽泓森高科林业股份有限公司 Locust tree radicula section seedling-raising technique
CN108093906A (en) * 2017-12-28 2018-06-01 湖北民族学院 A kind of Cutting root reproduction method of idesia female seedling
CN110959457A (en) * 2019-12-20 2020-04-07 漳州市林业科技推广站 Cutting seedling raising method for dalbergia odorifera

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Publication number Priority date Publication date Assignee Title
CN107306729A (en) * 2017-09-20 2017-11-03 上海市园林科学规划研究院 Asimina triloba buries root propagation method
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CN108093906A (en) * 2017-12-28 2018-06-01 湖北民族学院 A kind of Cutting root reproduction method of idesia female seedling
CN110959457A (en) * 2019-12-20 2020-04-07 漳州市林业科技推广站 Cutting seedling raising method for dalbergia odorifera

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Application publication date: 20170315