CN106482988A - A kind of Portable high-flow air sampler - Google Patents
A kind of Portable high-flow air sampler Download PDFInfo
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- CN106482988A CN106482988A CN201611149701.7A CN201611149701A CN106482988A CN 106482988 A CN106482988 A CN 106482988A CN 201611149701 A CN201611149701 A CN 201611149701A CN 106482988 A CN106482988 A CN 106482988A
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- 238000005070 sampling Methods 0.000 claims abstract description 49
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/2202—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/2273—Atmospheric sampling
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of Portable high-flow air sampler, including blower fan, collection pipeline, humidification sampling head, sampling media, bracing frame, battery and circuit control module, wherein, one end of collection pipeline is seamless with blower fan is fixedly connected, and the other end installs humidification sampling head；The position for being close to blower fan inside collection pipeline is provided with bracing frame, and sampling media is placed on bracing frame；By battery power fan operation；The particulate matter of in the air enters collection pipeline under blower fan driving, is sampled capture medium.The sampler has the advantages such as small, flow is big, collecting efficiency is high, can be widely applied to the sampling of various site environment aerosols, has good application prospect in fields such as environmental monitorings.
Description
Technical field
The present invention relates to a kind of air sampler, especially a kind of big flow, efficient and portable air sampling rig and
The method of sampling, can be widely applied to the fast of the acquisition testing of indoor and outdoor surroundingses particulate in air, particularly pathogenic microorganism
Fast examination and monitoring.
Background technology
Current air pollution is increasingly subject to worldwide concern, seriously threatens residents ' health.In the air is except existing
Chemical contamination beyond the region of objective existence, also substantial amounts of bioaerosol, such as bacterium, virus, fungal particles etc..Research shows that bioaerosol is sudden and violent
Dew can cause many health problems, such as infection in respiratory system, allergy etc., and the generation of bioaerosol, diffusion, propagation can more add
This harm of weight, (such as 2003 SARS, H1N1 in 2009, the acute epidemic situation such as H5N9 and 2015 year 2013 MERS are quick-fried
Send out).Therefore quick detection to bioaerosol or even real-time monitoring just become particularly important to find potential danger.But make
For the vital first step in bioaerosol quick detection or even real-time monitoring, effective collection of air sample is always
One difficult problem.
It is the Biosampler (SKC) and Anderson designed using collision theory to apply more two kind sampler at present
Sampler, the former are to gather air sample in liquid medium, overcome the problem of particulate matter bounce-back and secondary particulate,
Flow is 12.5L/min；The latter collects air sample on solid medium, is the bioaerosol collection side of international standard
Method, existing more than 50 years usage history, flow is 28.3L/min.But both approaches are required for aspiration pump and rely on alternating current,
It is difficult to portable use when participating in the cintest；In addition, both sampling flows are too low, it is impossible to which development and application is in quick detection or even the reality of surrounding air
When monitor.Meanwhile, Anderson sampler stroke speed is big (up to 24m/s), can produce impact damage to microorganism and cause
Culturability is lost, causes Bacterial diversity concentration to reduce, so as to cause to underestimate the concentration level in environment.
The rise of the gene amplification method with PCR as representative significantly reduces the Cmin of biology sample detection, improves
The efficiency of microorganism detection, the appearance of constant-temperature amplification method further shorten the reaction time, simplifies course of reaction；Meanwhile,
Some biology sensors based on electrochemistry occur can be by microorganism biological signal nothing (as silicon nanowires field-effect transistor)
Postpone to be converted into electric signal, so that the automatic on-line monitoring of microorganism becomes possible to.But, the concentration of in the air pathogenic bacteria has
When can be very low, particularly with some high disease-producing pathogens, the research and development of the rapid and handy sampler of high flow capacity are extremely important.Effectively sharp
Bioaerosol quick detection is carried out with existing technology at present, it is necessary to more powerful bioaerosol sampler is depended on,
The higher volume of air sample of collection in time as short as possible, while transitioned into liquid to carry out gene magnification or spy
Different in nature electrochemical reaction.
Content of the invention
The present invention is intended to provide the portable air particle sampler of a kind of super-flow (more than 1 cubic meters per minute),
More aerosol particles are gathered within the time as short as possible, be easy to subsequent analysis to detect.
The main technical principle of the present invention is：Using lithium battery as power supply, miniature Wind Volume blower fan drives air to lead to
Collection pipeline is crossed, duct bottom center fixture agar medium, the airborne particles thing for entering pipeline are struck on culture medium
Captured.For improving collecting efficiency further, humidification sampling head is installed additional in collection pipeline air inlet to overcome drying effect, make little
Particle moisture absorption increases, moreover it is possible to mitigates the impact damage to biologic grain further and mitigates the secondary ejection of the particle for collecting.
Technical scheme is as follows：
A kind of Portable high-flow air sampler, including blower fan, collection pipeline, humidification sampling head, sampling media, support
Frame, battery and circuit control module, wherein, one end of collection pipeline is seamless with blower fan to be fixedly connected, and the other end is installed humidification and adopted
Sample head；The position for being close to blower fan inside collection pipeline is provided with bracing frame, and sampling media is placed on bracing frame；The blower fan leads to
Oversampling circuit control module connects battery, by battery power fan operation.
For ease of gripping, the blower fan can set up on one panel, be installed for hand-held lever on panel.The face
Plate can be made using engineering plastics, and the lever can be Z-type one-handed grip, and material can be stainless steel, solid using tapping screw
On panel.
The blower fan is miniature Wind Volume blower fan, it is preferred that its a diameter of 140 ± 60mm, and rated voltage is 5～24V, volume
Electric current is determined for 0.3～6A, air quantity is 30～300CFM, most preferably diameter 12cm, rated voltage 12V, rated current 3A, air quantity
200CFM.
Further, the collection pipeline is preferably reducing type collection pipeline, installs humidification sampling head one end (i.e. air intake
Mouthful) bore less, the bore of connecting fan one end (i.e. air outlet) is larger.
Picking and placeing for ease of collection medium, forms half in the crosscutting processing of collection pipeline bottom (near blower fan one end) side wall
Cylinder, the semicircular cylinder and parent pipeline one end gemel connection, (line of hinge and lock is conduit cross-sectional for other end lock connection
Face diameter of a circle), allow semicircular cylinder outwards Unscrew.On the inwall of the semicircular cylinder that outwards can be rotated, screw can be passed through
A support pallet is fixed as the bracing frame for carrying sampling media.In one embodiment of the invention, the support pallet position
The position of about 1cm, is a round tray above the blower fan, and its center of circle is overlapped with the collection pipeline cross circular section heart, and diameter is bigger
In culture dish to place and film solid media, when the outside Unscrew of the semicircular cylinder, training of can easily placing and take
Foster base.
The material of collection pipeline can be metal (such as stainless steel, aluminium alloy) or plastics (such as PVC, ABS, PC) etc., but manage
Road inwall is polytetrafluorethylecoatings coatings.Air inlet internal diameter is preferably 10～100mm, and air outlet internal diameter is preferably 80～200mm.?
In one embodiment of the present of invention, the collection pipeline is polytetrafluoroethylene (PTFE) material, air inlet internal diameter 50mm, air outlet internal diameter
100mm, the high 20mm of the semicircular cylinder of crosscutting formation.
The humidification sampling head is preferably ring-like pipe-like container, and which is internally provided with one or more for ultrasonic atomizatio
Atomizing piece, corresponding, humidification sampling head inner wall lower symmetric configuration water smoke spray orifice, atomizing piece by sonic oscillation will manage
Aseptic water atomization in road container is sprayed through spray orifice.The internal diameter of humidification sampling head gathers the external diameter of pipeline air inlet, Ke Yian
It is attached to collection pipeline air inlet.In one embodiment of the invention, the internal-and external diameter difference of humidification sampling head ring pipe is 10mm,
Highly 10～50mm, the water smoke injection diameter on inwall are 5mm or so.
The sampling media can be Solid agar culture, for gathering the educable microorganism of in the air, including thin
Bacterium culture medium and fungi culture medium, for example：TSA bacteria culture media is used for gathering bacterial aerosol, and sabouraud culture medium is used for gathering
Fungi aerosol.
The sampling media can also be liquid, for gathering the suspended particulate substance of in the air, can including but not limited to train
Foster microorganism.Liquid sample medium is preferably not volatile liquid, such as sterilized water, fluid nutrient medium, organic solvent, grease
Deng.
The battery is preferably lithium ion chargeable battery, and such as voltage is 12V, and capacity is the lithium-ion electric of 20000mAh
Pond.
It is single-chip microcomputer that the circuit of the sampler controls preferred master control core.Control circuit adopts speed and current double closed loop
Control mode is to strengthen mechanical property and the interference rejection ability of motor, it is ensured that rotation speed of fan is constant, so as to by arranging the sampling time
Realize the sampling of quantitative air volume.Except atomizing piece is located in humidification sampling head, other are used for the circuit of ultrasonic atomizatio and element
It is integrated on circuit board.
Beneficial effects of the present invention：The Portable high-flow air sampler that the present invention is provided overcomes current air sampling
On some defects, for example existing flow existed with golden mark method Anderson sampler is low, need external air pump, surface
The problems such as impact damage is serious, drying effect is notable, the present invention drive air using open impacting type sampling method by blower fan
Culture medium is clashed into, the efficient sampling to biologic grain thing is realized, sampler is small, flow is big, collecting efficiency is high, can be extensive
The sampling of environmental organism aerosol is applied to, has good application prospect in fields such as environmental monitorings.It is mainly characterized by：
(1) the sampler flow is big, reaches 1000L/min, but the stroke speed to culture medium is less than 10m/s, during unit
The quantity of the interior Bacterial diversity for collecting is greatly increased, sampler miniature portable, can be existing in environmental organism aerosol
Widely use in terms of quarry sampling and experiment chamber microbiological culture.
(2) increase little particle moisture absorption using humidification sampling head, collecting efficiency is improved, while mitigate hitting to biologic grain
Hit damage；
(3), when sampling media is changed to liquid, the sampler can be in conjunction with biochemistry such as enzyme linked immunological, PCR and constant-temperature amplifications
Analysis method can carry out effective detection to the biotic component of in the air.
Description of the drawings
Fig. 1 is the structural representation of Portable high-flow sampler described in the embodiment of the present invention, and wherein A is side view, and B is
Top view；1- blower fan, 2- gather pipeline, and 3- humidifies sampling head, 4- sampling media, 5- bracing frame, 6- battery, 7- lever, 8- face
Plate, 9- screw, 10- are latched, 11- control circuit.
Fig. 2 is the structure schematic top plan view of bracing frame and pipeline connection, and wherein 2- gathers pipeline, and 5- bracing frame, 10- are locked
Button, 12- screw, 13- hinge.
Fig. 3 shows the biology of Portable high-flow samplers sample in the air bacteria particles thing described in the embodiment of the present invention
Property efficiency.
Specific embodiment
Below in conjunction with the accompanying drawings, the present invention is described in further detail by embodiment.
Fig. 1 is the structural representation of Portable high-flow air sampler.The sampler is by mini fan 1 and sampling conduit
2 constitute column type sample space.The optimum diameter of blower fan 1 is 120mm, and rated voltage is 12V, and rated current is 3A, and air quantity is
200CFM.Blower fan 1 is fixed on certain altitude on panel 8 using 4 M5 screws 9, is not less than 40mm.Panel 8 adopts ABS engineering
Plastics, firm solid and easy to process, a size of 120mm × 200mm, thick 5mm.Collection pipeline 2 gathers pipeline for reducing type, interior
Footpath becomes 100mm for 50mm.Reducing type collection 2 seamless binding of pipeline makes collection pipeline 2 just to blower fan on blower fan 1.Collection pipeline
Humidification sampling head 3 is placed in 2 tops, and 3 height of humidification sampling head is 50mm, internal equipped with two atomizing pieces for being used for ultrasonic atomizatio,
And two water smoke spray orifices of inner wall lower symmetric configuration.It is bracing frame 5 at collection center of 2 bottom of pipeline at blower fan 10mm, uses
In placement culture medium, i.e. sampling media 4.Bracing frame 5 is round tray, as shown in Fig. 2 its center of circle and collection pipeline cross circular section
The heart overlaps, and is fixed on the semicircular cylinder that outwards can be rotated being processed into by screw 12, and semicircular cylinder is passed through with parent pipeline one end
Hinge 13 connects, and the other end is connected by lock 10, and outside Unscrew is conveniently placed and take culture medium.
The capacity of lithium battery 6 is 20000mAh, can drive sampler continuously work more than 6 hours.Control circuit 11
Master control core is single-chip microcomputer, and control mode is speed and current double closed loop, is corrected by test, and the flow for arranging sampler is
1000L/min, sampling time (sampled air volume) arbitrarily can be arranged, generally 1-10min (1m3-10m3).Place training
After foster base, switch is opened, start after selecting the suitable sampling time and start sampling.Blower fan drives air from sampling conduit air inlet
(humidifying sampling head one end) enters pipeline, and the water smoke that the particulate matter carried by air-flow is sprayed with water smoke spray orifice meets and mixes, gas
Stream carries particulate matter and impinges upon so as to captured on culture medium.Heart stroke speed is 8.32m/s to actual measurement particulate matter in the medium,
And 2.35m/s is outwards gradually decrease to along culture medium radius, fast much smaller than the shock of the 24m/s of Anderson sampler on the whole
Degree.
Shown in Fig. 3 is the experimental result of the Portable high-flow samplers sample outdoor air cultivable bacteria.From figure
In as can be seen that being above golden mark using the cultivable bacteria sum obtained by the samplers sample 1 minute, 5 minutes, 10 minutes
For the 10 minutes cultivable bacteria sum collected by method Anderson sampler.The Portable high-flow sampling as can be seen here
The collecting efficiency of device is greatly improved, and can greatly shorten the time of sampling when detecting at the scene.
Claims (10)
1. a kind of Portable high-flow air sampler, including blower fan, collection pipeline, humidifies sampling head, sampling media, support
Frame, battery and circuit control module, wherein, one end of collection pipeline is seamless with blower fan to be fixedly connected, and the other end is installed humidification and adopted
Sample head；The position for being close to blower fan inside collection pipeline is provided with bracing frame, and sampling media is placed on bracing frame；The blower fan leads to
Oversampling circuit control module connects battery, by battery power fan operation.
2. Portable high-flow air sampler as claimed in claim 1, it is characterised in that the blower fan is erected at a face
On plate, it is provided with panel for hand-held lever.
3. Portable high-flow air sampler as claimed in claim 2, it is characterised in that the panel material is engineering plastic
Material；The lever is Z-type one-handed grip, and material is stainless steel, is fixed on panel by tapping screw.
4. Portable high-flow air sampler as claimed in claim 1, it is characterised in that the blower fan is miniature Wind Volume
Blower fan, its a diameter of 140 ± 60mm, rated voltage are 5～24V, and rated current is 0.3～6A, and air quantity is 30～300CFM.
5. Portable high-flow air sampler as claimed in claim 1, it is characterised in that the collection pipeline is reducing type
Collection pipeline, it is the less air inlet of bore to install humidification sampling head one end；Connecting fan one end is the larger air outlet of bore.
6. Portable high-flow air sampler as claimed in claim 5, it is characterised in that the material of the collection pipeline is
Metal or plastics, inwall are polytetrafluorethylecoatings coatings, and air inlet internal diameter is 10～100mm, and air outlet internal diameter is 80～200mm.
7. Portable high-flow air sampler as claimed in claim 1, it is characterised in that in collection pipeline near blower fan one
The crosscutting processing of the side wall at end formed one can outside Unscrew semicircular cylinder, the semicircular cylinder and parent pipeline one end gemel connection,
Other end lock connection.
8. Portable high-flow air sampler as claimed in claim 6, it is characterised in that support frame as described above is a supporting bracket
Disk, its side are fixedly connected with the inwall of the semicircular cylinder by screw.
9. Portable high-flow air sampler as claimed in claim 1, it is characterised in that the humidification sampling head is arranged on
Collection pipeline air inlet, is ring-like pipe-like, and the atomizing piece being internally provided with for ultrasonic atomizatio, inner wall lower correspond to layout
Water smoke spray orifice, sonic oscillation make the aseptic water atomization in pipeline and spray through water smoke spray orifice, mix with the air for entering collection pipeline
Close.
10. Portable high-flow air sampler as claimed in claim 1, it is characterised in that the sampling media is solid
Culture medium or not volatile liquid；The battery is lithium ion chargeable battery；The circuit control module is single-chip microcomputer control
Circuit processed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611149701.7A CN106482988A (en) | 2016-12-13 | 2016-12-13 | A kind of Portable high-flow air sampler |
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| CN201611149701.7A CN106482988A (en) | 2016-12-13 | 2016-12-13 | A kind of Portable high-flow air sampler |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106918476A (en) * | 2017-04-21 | 2017-07-04 | 苏州尚科洁净技术有限公司 | A kind of big flow ram for air sampling |
| CN111500427A (en) * | 2020-03-21 | 2020-08-07 | 深圳市朗司医疗科技有限公司 | Portable wet wall cyclone microorganism aerosol collector |
| CN112226345A (en) * | 2020-09-25 | 2021-01-15 | 闽江学院 | In-situ culture device and collection method for aerosol microorganisms on ocean air-sea interface |
| CN112903375A (en) * | 2021-01-19 | 2021-06-04 | 南京信息工程大学 | Aerosol collecting and enriching instrument and working method |
| CN113310754A (en) * | 2021-06-09 | 2021-08-27 | 华南师范大学 | Atmospheric particulate continuous classification wet sampling device |
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