CN106480166A - One class detects the application of the high specific fluorescent probe of carboxy-lesterase 1 - Google Patents
One class detects the application of the high specific fluorescent probe of carboxy-lesterase 1 Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
Abstract
One class detection carboxy-lesterase 1（Carboxylesterase 1, CE1）High specific fluorescent probe application, it belongs to field of fine chemical.This Specific probe is 2 hydroxyl 3 pi-allyl benzo azole compounds esters derivative, and it can be used for detecting the quantitative determination of the presence or absence of CE1 and its vigor in different biological samples.It is as follows that enzyme activity specifically measures flow process：Select 2 hydroxyl 3 pi-allyl benzothiazole esters derivative hydrolysis to be probe reaction, select suitable substrate concentration in linear reaction interval by the growing amount of its hydrolysis metabolite 2 hydroxyl 3 pi-allyl benzothiazole in the detection by quantitative unit interval measure each biological sample, cell, in body and overall organ CE1 enzyme substantial activity.This probe cannot be only used for the qualitative assessment of CE1 enzyme activity in separate sources biological specimen, can be additionally used in the inhibitor of external rapid screening CE1 in addition by this probe reaction.
Description
Technical field
The present invention relates to a class detects the high specific fluorescent probe of carboxy-lesterase 1 (Carboxylesterase 1, CE1)
Application, it belongs to field of fine chemical.
Background technology
Carboxy-lesterase 1 (carboxylesterase 1, CE1), is a kind of broad spectrum activity serine protease, participates in a lot of medicines
Thing and the metabolism of noxious substance, and participate in some other physiological process.CE1 mainly expresses in liver, in small intestinal, kidney, lung, testis
There are a small amount of distribution, CE1 and carboxy-lesterase 2 (carboxylesterase 2 in ball, heart；CE2), there is 47% sequence homology
Property, but its tissue distribution and preference substrate structure feature substantially differ from CE2.CE1 catalysis triangle includes Ser-221, Glu-
353, and His-467, it is related to two-step reaction mechanism.The first step, formed under serine residue effect covalent acetyl-
Enzyme intermediate, discharges containing alcoholic extract hydroxyl group portion of product simultaneously.Second step, a molecular water attack acetyl-enzyme intermediate, release simultaneously
Release carboxy moiety product.If other compound attack acetyl-enzyme intermediate, acyl group transfer reaction will occur, rather than
Hydrolysis.
The major function of CE1 is xenobiotic metabolism, and it can hydrolyze containing structures such as carboxylic acid ester bond, amido link, thioester bonds
Compound.And, some clinical medicines of CE1 energy metabolism, including ***e, morphine, Pethidine, dolantin and benefit card
Cause.Additionally, CE1 can be by some prodrugs, such as lovastatin is hydrolyzed to its form active in vivo.CE1 is by R-
The bonded partial hydrolysiss of methyl ester in ***e, (main metabolic of the ***e that can detect in urine is produced to generate ecgonine benzoate.
Thing).When ***e and ethanol are simultaneous, CE1 energy metabolism ***e generates noxious substance ***e second alkali.
The second largest biological function of CE1 is during the cholesterol and fatty acid of metabolism and transport liver and its periphery
Play a significant role.Although there being some other enzymes to participate in these processes, researcher finds, in mononuclear cell, huge bite thin
In born of the same parents and hepatocyte, CE1 has non-gallbladder-salt-dependent, also referred to as cholesterol hydrolase (cholesteryl ester
Hydrolase, CEH), the process that cholesterol is transported to liver from blood vessel wall is referred to as-macrophage reverse cholesterol transport ",
It is the process restoring atherosclerotic lesions, and the cholesterol ester hydrolysis of CHE catalysis, it is that cholesterol regulating is transferred out of huge biting
The rate-limiting step of cell processes.Additionally, CE1 has fatty acetyl-CoA hydrolase function, CE1 catalysis acyl CoA and ethanol
The fatty acyl ethyl ester (FAEEs) generating and alcoholic hepatic necrosis damage closely bound up.
The third-largest biological function of CE1 be in endoplasmic reticulum by N linked glycosylation site modification after, have exchange and retain egg
Contour painting energy.In endoplasmic reticulum, CE1 is directly combined with C reactive protein (C-reactive protein, CRP), until CRP release
To in blood.The release of CRP is related to tissue injury, and so far, it is the most sensitively to detect atherosclerotic mark
Thing.Additionally, CE1 is equally combined with the II phase drug metabolism enzyme GRD beta-glucuronidase in endoplasmic reticulum, GRD beta-glucuronidase is used
In removing different in nature biomass, endogenous material, the outer row of the such as chemical combination beyond the region of objective existence of organophosphorus compoundses is associated.
Additionally, CE1 can be used as early hepatocarcinoma tumor markerses.Na etc. is by entering to the hepatic tissue sample of 58 liver cancer patients
Row research finds, compared with normal liver tissue, in liver cancer tissue, CE1 expression substantially reduces, and shows that the growth of hepatoma carcinoma cell can
Can suppress the expression of CE1.Also find that the content of CE1 in liver cancer patient blood serum is significantly raised recently, CE1 is likely to become a kind of latent
Hepatocarcinoma early diagnosis blood serum designated object, the detection to CE1 is significant.
The invention provides the esters derivative of class 2- hydroxyl -3- pi-allyl benzo azole compounds and its conduct
The application of CE1 enzyme fluorescent probe substrate, it can generate, after carboxy-lesterase CE1 hydrolysis, the water that fluorescence emission spectrum is different from prototype
Solution product.This enzymatic reaction has that selectivity is high, metabolite easily detects, enzyme activity and inhibitory activity evaluate the spy such as rapidly and efficiently
Point.
Content of the invention
It is an object of the invention to provide a class detects high specific fluorescent probe and its application of carboxy-lesterase 1 (CE1),
The Fluorescence Fluorescence attribute of this substrate prototype and hydrolyzate has notable difference.Can be to multiple living things systems using this probe reaction
The distribution of middle CE1 and function carry out quantitative assessment.
It is an object of the invention to provide a class detects high specific fluorescent probe and its application of carboxy-lesterase 1 (CE1),
The ester bond of this substrate can be corresponding hydrolyzate by CE1 specific for hydrolysis in human tissue or cell, and product has fluorescence；
This substrate is with 2- hydroxyl -3- pi-allyl benzo azole compounds as raw material, obtains corresponding 2- hydroxy ester by esterification
Analog derivative, shown in its general structure such as formula (1), wherein, R is methyl, ethyl, n-pro-pyl, normal-butyl, n-pentyl, n-hexyl,
Phenyl, p-methylphenyl, to ethylphenyl, to propyl group phenyl, to hexyl propyl group, p-nitrophenyl, rubigan, 1- how base,
One of 2- furyl, 2- thienyl, (4- phenyl) phenyl, 4- ethoxyl phenenyl substituent group.
As when R is for n-pro-pyl, this substrate is 2- butyryl acyloxy 3- pi-allyl benzothiazole.
Present invention also offers the application of the specificity fluorescent probe substrate of CE1, this substrate is as the specificity bottom of CE1
, there are hydrolysis, enzyme or cell preparation solution etc. measured by the growing amount of hydrolyzate in the detection by quantitative unit interval in thing
The activity of CE1 in biological sample and cell；Specifically assay method is：
Using the esters derivative of 2- hydroxyl -3- pi-allyl benzo azole compounds 2- hydroxyl as special in system
Property probe substrate；Concentration of substrate selects 1/10～10Km；Concentration of substrate preferred K during single point assaym.
In the conventional buffer such as PBS or Tris-HCl, between reaction temperature is 20 DEG C to 60 DEG C, preferably 37 DEG C are
The peak optimization reaction time；Between 5.5～10.5, preferably pH 7.4 is peak optimization reaction pH value to incubation system pH；
Response time is 5～120 minutes, is guaranteeing that the corresponding hydrolyzate of above substrate reaches quantitative limit and substrate
Terminating reaction when conversion ratio is less than 20%；
In the analytical unit time, hydrolyzate growing amount is as the evaluation index of carboxy-lesterase CE1 activity.
Application, described substrate elimination factor or the hydrolysis of the human carboxylatase 1 specificity fluorescent probe substrate that the present invention provides
The production rate of product should be between 0.1%～20%.
Application, probe substrate and its hydrolyzate of the human carboxylatase 1 specificity fluorescent probe substrate that the present invention provides
It is respectively provided with fluorescence properties, the rapid sensitive detection of product and substrate can be realized using fluorescence detector；Fluoroscopic examination condition is：Swash
Send out wavelength 330nm, carry out the detection of fluorescence emission spectrum in 450～600nm.Additionally, this Specific probe and corresponding CE1 live
Property detection process will not be disturbed by living things system substrate and impurity, can be used for CE1 enzyme activity in various living things systems quantitation survey
Fixed.
The reaction of this specific probe can be used for recombinating in carboxy-lesterase, people and animal tissue's preparation solution and various
The quantitative determination of CE1 enzyme activity, it may also be used for the inhibitor of rapid screening CE1 enzyme and the quantitative assessment of rejection ability.
Using the restructuring mono- enzyme of carboxy-lesterase CE1, liver microsomes incubation system investigated, by correlation analysiss, specifically
Property Inhibition test, the single enzyme metabolic response of restructuring, and many evidences such as enzyme kinetics are it was demonstrated that 2- hydroxyl -3- allyl
The esters derivative of yl benzazoles compound 2- hydroxyl can specific through carboxy-lesterase CE1 metabolism, generate corresponding water
Solution product.
As the fluorescent probe substrate of the carboxy-lesterase CE1 of high specific, this compound can be used to detect carboxy-lesterase
The activity of CE1, is especially suitable for antibacterial, insect cell, mammalian cell and yeast clonal expression system are produced
Carboxy-lesterase CE1 recombinase enzyme activity determination, and the tissue microsomal of multiple mammalian tissues organ origin, S-9 etc.
The activity demarcation of CE1 in prepared product.
Specific probe detection carboxy-lesterase CE1 enzyme external activity tool from carboxy-lesterase CE1 of the present invention
There is advantage following outstanding：
(1) high specific：The esters derivative of 2- hydroxyl -3- pi-allyl benzo azole compounds 2- hydroxyl can be by carboxylic acid
Esterase CE1 is metabolized to a metabolite with high specificity, i.e. the hydrolyzate of 2 ester linkage breakings.
(2) cheap and easy to get：The esters derivative of 2- hydroxyl -3- pi-allyl benzo azole compounds 2- hydroxyl and its hydrolysis
Product all can obtain through chemosynthesis, and synthesis technique is simple.
(3) high sensitivity：Have 2- hydroxyl -3- pi-allyl benzothiazole mother nucleus structure compound be respectively provided with good glimmering
Optical emission spectroscopy characteristic (450～600nm), this substrate and its hydrolysis metabolite have different fluorescence emission spectrum signatures,
Detection can preferably be made a distinction, can be quantitative determined by drawing standard curve simultaneously.
Brief description
The mono- enzyme selectivity of Fig. 1.In figure numeral represents following (1 respectively：Carbon neuraminidase, 2：Pepsin, 3：Trypsin,
4：Digestive Enzyme, 5：A- Chymotrypsin (a-CT) 6：E.C. 3.4.21.64,7：Retinol binding protein, 8：Lysozyme (Lozyme), 9：Acid
Glycoprotein, 10：Acetylcholinesterase, 11：Butyrylcholine esterase, 12：Human serum albumin, 13：Bovine serum albumin, 14：Carboxylic
Acid esters enzyme 2,15：Carboxy-lesterase 1,16：Buffer solution)
Fig. 2 2- butyryl acyloxy -3- pi-allyl benzothiazole and carboxy-lesterase 1 concentration linear relationship.
Fig. 3 carboxy-lesterase 1 hydrolysis 2- butyryl acyloxy -3- pi-allyl benzothiazole speed and hydrolysis clopidogrel speed phase
Guan Xing.
Fig. 4 2- butyryl acyloxy -3- pi-allyl benzothiazole cytotoxicity.
Fig. 5 2- butyryl acyloxy -3- pi-allyl benzothiazole cell two photon imaging.
Specific embodiment
The following examples will be further described to the present invention, not thereby limiting the invention.
The synthesis of embodiment 1 2- acetoxy-3-pi-allyl benzothiazole (AABO)
Under nitrogen protection, will be equipped with 0.5mmol 2- hydroxyl -3- pi-allyl benzothiazole, 0.6mmol triethylamine and 10mL
The 25mL two-mouth bottle of dichloromethane is placed in ice bath, and 0.75mmol chloroacetic chloride is dissolved in 5mL dichloromethane, in 30min, slowly
It is added drop-wise in bottle, after stirring 1h under ice bath, be stirred overnight at room temperature.Reactant liquor adopts column chromatography to separate, and (developing solvent is acetic acid second
Ester: petroleum ether=1: 5, v: v), obtain 117.7mg colourless transparent liquid (yield, 80.3%).1H NMR(400MHz,CDCl3)δ
8.16 (1H, dd, J=7.8,1.4), 7.74 (1H, dd, J=6.1,3.0), 7.59 7.49 (1H, m), 7.44 (1H, d, J=
6.5), 7.39 7.30 (3H, m), 6.07 5.81 (1H, m), 5.14 (2H, dd, J=12.3,5.8), 3.42 (2H, d, J=
6.6),2.49(3H,s).13C NMR(100MHz,CDCl3)δ169.68,160.04,150.13,147.62,142.02,
135.42,134.35,133.34,128.47,126.35,125.39,124.54,120.56,120.31,116.83,110.43,
34.64,21.30.HRMS(ESI positive)[M+H]+Theoretical value 294.1125, measured value 294.1128.
The synthesis of embodiment 2 2- butyryl acyloxy -3- pi-allyl benzothiazole (BABO)
Under nitrogen protection, will be equipped with 0.5mmol 2- hydroxyl -3- pi-allyl benzothiazole, 0.6mmol triethylamine and 10mL
The 25mL two-mouth bottle of dichloromethane is placed in ice bath, and 0.75mmol butyl chloride is dissolved in 5mL dichloromethane, in 30min, slowly
It is added drop-wise in bottle, after stirring 1h under ice bath, be stirred overnight at room temperature.Reactant liquor adopts column chromatography to separate, and (developing solvent is acetic acid second
Ester: petroleum ether=1: 5, v: v), obtain 109.7mg colourless transparent liquid (yield, 68.3%).1H NMR(400MHz,CDCl3)δ
8.13 (d, J=7.7Hz, 1H), 7.83 7.74 (m, 2H), 7.57 (d, J=7.4Hz, 1H), 7.50 7.39 (m, 3H), 5.85
5.95 (m, 1H), 5.10 (t, J=12.8Hz, 2H), 3.38 (d, J=6.5Hz, 2H), 2.75 (t, J=7.2Hz, 2H), 1.78
1.64 (m, 2H), 1.00 (t, J=7.4Hz, 3H).13C NMR(100MHz,DMSO-d6)δ172.05,159.98,150.04,
147.57,141.59,136.14,134.66,134.26,128.65,127.07,126.36,125.45,120.41,117.14,
111.31,35.93,34.41,18.06,14.09.HRMS(ESI positive)[M+H]+Theoretical value 322.1438, measured value
322.1430.
The synthesis of embodiment 3 2- benzoyloxy -3- pi-allyl benzothiazole (BZABO)
Under nitrogen protection, will be equipped with 0.5mmol 2- hydroxyl -3- pi-allyl benzothiazole, 0.6mmol triethylamine and 10mL
The 25mL two-mouth bottle of dichloromethane is placed in ice bath, and 0.75mmol Benzenecarbonyl chloride. is dissolved in 5mL dichloromethane, in 30min, delays
Slowly it is added drop-wise in bottle, after stirring 1h under ice bath, be stirred overnight at room temperature.Reactant liquor adopts column chromatography to separate, and (developing solvent is acetic acid
Ethyl ester: petroleum ether=1: 5, v: v), obtain 77.4mg weak yellow liquid (yield, 43.6%).1H NMR(400MHz,CDCl3)δ
8.20 8.31 (3H, m), 7.69 (1H, t, J=7.2), 7.63 7.46 (4H, m), 7.42 (1H, dd, J=14.6,7.0),
7.33 7.16 (3H, m), 6.01 (1H, m), 5.22 5.03 (2H, m), 3.49 (2H, d, J=6.6).13C NMR(100MHz,
CDCl3)δ165.45,160.29,150.30,147.76,141.69,138.59,136.49,135.37,134.55,133.49,
133.38,132.82,130.41,128.97,128.61,126.48,125.17,124.40,120.19,116.95,110.31,
34.66.HRMS(ESI positive)[M+H]+Theoretical value 356.1281, measured value 356.1290.
Selectivity in the recombinant expressed people's list enzyme of embodiment 4
Reaction is carried out in PBS (pH=7.4,100mM), is to be separately added into following hydrolysis in 200 μ L systems in final volume
Enzyme or desmoenzyme：Carbon neuraminidase (CA), pepsin (Pepsin), trypsin Trypsin), Digestive Enzyme (Lipase), a-
Chymotrypsin (a-CT), E.C. 3.4.21.64 (Proteinase k), retinol-binding proteins (Retinol binding protein
4, RBP4), lysozyme (Lozyme), acidoglycoprotein (AAG), acetylcholinesterase (AChE), butyrylcholine esterase (BChE),
Human serum albumin (HSA), bovine serum albumin (BSA), carboxy-lesterase 2 (CE2) and carboxy-lesterase 1 (CE1), are separately added into
AABO, BABO and BZABO (final concentration is all 40 μM) are incubated 30min altogether, are subsequently adding 200 μ L ice acetonitrile terminating reactions, mix,
20000 × g is centrifuged 5min, takes 150 μ L of supernatant liquid, is detected using microplate reader.(see Fig. 1)
Embodiment 5 is recombinated the protein concentration of CE1 catalyzed linear reaction in single enzyme
Reaction is carried out in PBS (pH=7.4,100mM), is to be separately added into variable concentrations in 200 μ L systems in final volume
Carboxy-lesterase 1 (0.5,1.0,1.5,2.0,3.0,4.0,6.0 μ g/mL), adds BABO (40 μM of final concentration) to be incubated 15min altogether,
It is subsequently adding 200 μ L ice acetonitrile terminating reactions, mixes, 20000 × g is centrifuged 5min, takes 150 μ L of supernatant liquid, is entered using microplate reader
Row detection.Calculate the linear protein concentration (see Fig. 2) of recombined human CE1 enzyme.Linearity curve equation is Y=587.7*X+264.7 (R2
=0.9960), wherein Y represents fluorescence intensity, and X represents carboxy-lesterase 1 concentration.
The Chemical Inhibition experiment of embodiment 6 probe BABO
Reaction is carried out in PBS (pH=7.4,100mM), and reaction system is 200 μ L, 40 μM of BABO final concentration, carboxylate
Enzyme 1 and the final concentration of 1 μ g/mL of people's hepatomicrosome, 4 kinds of inhibitor (BNPP, LPA, HA, EDTA) concentration of selection are 100 μ
M.The suppression percentage ratio that reduced by fluorescence intensity level (I500) of fraction is representing.
Embodiment 7 carboxy-lesterase 1 hydrolysis BABO is tested with clopidogrel Rate-dependency
Choose 12 people's hepatomicrosomes, investigate the dependency of its hydrolysis BABO speed and clopidogrel speed.BABO reacts
Condition is：Reaction is carried out in PBS (pH=7.4,100mM), is to add different people hepatomicrosome in 200 μ L systems in final volume
(final concentration 1 μ g/mL) and BABO (40 μM of final concentration) are incubated 15min altogether, are subsequently adding 200 μ L ice acetonitrile terminating reactions, mix,
20000 × g is centrifuged 5min, takes 150 μ L of supernatant liquid, is detected using microplate reader；Clopidogrel reaction condition is：Substrate 50 μ
M, hepatomicrosome 100 μ g/mL, react 30min；Clopidogrel and hydrolyzate are using using high performance liquid chromatography-ultraviolet combination
Method is analyzed, and Detection wavelength is 240nm.The hydrolysis rate of the hydrolysis rate of BABO and clopidogrel has been carried out dependency plan respectively
Close, correlation coefficient adopts the coefficients R of equation of linear regression2Represent, P<0.005 has significant difference.(see Fig. 3)
Embodiment 8 probe BABO cytotoxicity detects
Measure the cell of probe BABO using 3- (4,5- dimethylthiazole -2) -2,5- diphenyltetrazolium bromide bromide (MTT)
Toxicity.Choose the A549 cell being in exponential phase, the probe BABO of variable concentrations is added in culture medium, be incubated 48h
Afterwards, culture medium is changed to PBS, adds MTT solution (final concentration 0.5mg/L), containing 5%CO2After 37 DEG C of culture 4h in incubator
Add DMSO dissolving, measure the absorbance at 490nm.(see Fig. 4)
Embodiment 9 quantitative determines the activity of CE1 in human pulmonary epithelial cells
A549 cell MEM/EBSS cultivates base (containing 10%FBS) and cultivates, when cell covers with 70% culture bottle, at digestion
Reason, and planted in copolymerization Jiao's capsule with the concentration in 1 × 105/hole, it is placed in containing 5%CO2Incubator, 37 DEG C of incubated overnight.
The attached cell no culture medium of FBS, adds 37 DEG C of probe BABO (20 μM) to continue culture 30min, wherein suppresses after rinsing 2 times
Group adds inhibitor BNPP (100 μM) preincubate 30min in advance, adds probe culture.Cell rinses after 3 times through PBS, is placed in
Observe under two-photon Laser Scanning Confocal Microscope.Excitation wavelength 700nm, fluorescent collecting scope 495-540nm (see Fig. 5).
Claims (5)
1. one class detect carboxy-lesterase 1 (CE1) high specific fluorescent probe application it is characterised in that：The ester bond of this probe
The corresponding fluorescence of product for corresponding hydrolyzate and can be produced by CE1 specific for hydrolysis；This probe is as the specificity bottom of CE1
Thing, using its hydrolysis reaction activity, is measured in different enzyme sources by the growing amount of hydrolyzate in the detection by quantitative unit interval
The activity of CE1；
Formula（1）
This probe is 2- hydroxyl -3- pi-allyl benzothiazole esters derivative, its general structure such as formula（1）Shown, wherein R is first
Base, ethyl, n-pro-pyl, normal-butyl, n-pentyl, n-hexyl, phenyl, p-methylphenyl, to ethylphenyl, to propyl group phenyl, right
Hexyl propyl group, p-nitrophenyl, rubigan, 1- naphthyl, 2- furyl, 2- thienyl,（4- phenyl）Phenyl, 4- ethoxybenzene
One of base substituent group.
2. a class according to claim 1 detects the application of the high specific fluorescent probe of carboxy-lesterase 1, and its feature exists
In：The mono- enzyme of CE1, human or animal tissues preparation solution or various biological sample that described enzyme source is recombinant expressed.
3. a class according to claim 1 detects the application of the high specific fluorescent probe of carboxy-lesterase 1, and its feature exists
In：Described hydrolysis reaction system pH is between 5.5 ~ 10.5；The concentration of probe substrate is between 1/10 ~ 10K mBetween；Incubate
Educate system reaction temperature between 20 ~ 60oBetween C, the conversion ratio of hydrolyzate should be between 0.1% ~ 20% simultaneously.
4. a class according to claim 1 detects the application of the high specific fluorescent probe of carboxy-lesterase 1, and its feature exists
In：Described probe substrate does not possess fluorescence and its hydrolyzate has fluorescence properties, can using fluorescence detector realize product and
The rapid sensitive detection of substrate；Fluoroscopic examination condition is：Excitation wavelength 300 ~ 400 nm, carries out fluorescence in 450 ~ 600 nm
The detection of emission spectra.
5. a class according to claim 1 detects the application of the high specific fluorescent probe of carboxy-lesterase 1, and its feature exists
In：Described probe substrate and its hydrolysis can be used for the rapid screening of CE1 inhibitor and the quantitative assessment of rejection ability.
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| CN110294720A (en) * | 2018-03-23 | 2019-10-01 | 香港理工大学深圳研究院 | For detecting fluorescence probe and its application of butyrylcholine esterase |
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| CN104342106A (en) * | 2013-07-29 | 2015-02-11 | 中国科学院大连化学物理研究所 | Human carboxylesterase subtypes specific fluorescent probe and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110294720A (en) * | 2018-03-23 | 2019-10-01 | 香港理工大学深圳研究院 | For detecting fluorescence probe and its application of butyrylcholine esterase |
| CN110294720B (en) * | 2018-03-23 | 2021-05-07 | 香港理工大学深圳研究院 | Fluorescent probe for detecting butyrylcholine esterase and application thereof |
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Application publication date: 20170308 |