CN106479863B - For making the pushing device of cell monolayer - Google Patents
For making the pushing device of cell monolayer Download PDFInfo
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- CN106479863B CN106479863B CN201611167340.9A CN201611167340A CN106479863B CN 106479863 B CN106479863 B CN 106479863B CN 201611167340 A CN201611167340 A CN 201611167340A CN 106479863 B CN106479863 B CN 106479863B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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Abstract
The invention discloses a kind of for making the pushing device of cell monolayer, comprising: substrate, two sides are equipped with guide rail bar；Cell liquid is laid on substrate in a manner of slidable movement by push jack in guide rail bar；Push jack folder, connect with push jack；First axle component comprising have first axle mounting base and first axle；First axle component realizes connection by fixation hole and push jack folder；Second hinge component comprising have second hinge mounting base and second hinge；Second hinge component is connect with first axle component；The direction of propulsion of first axle and second hinge spatially Relative vertical.The case where using the push jack posture automatic fine tuning technology on double freedom, the quick continuous single layer for realizing 1,000,000,000 cells is spread out for this case, the superposition of no multi-layer cellular.The pushing device can be used cooperatively with imaging device, rapidly find out rare cell and sick cell, be of great significance to the early clinic detection of communicable disease and tumor and cancer.
Description
Technical field
It is the present invention relates to a kind of pushing device, in particular to a kind of for making the pushing device of cell monolayer.
Background technique
How rare cell and the micro- life of trace cause of disease fast and accurately identified in billions of a cells in peripheral blood
Object, be life science important subject and EARLY RECOGNITION communicable disease, tumor and cancer etc. it is extremely effective
Means are detected relative to traditional iconography, and detection time shifts to an earlier date significantly, thus it is for medical test, appliance for clinic examination
There is very important meaning.
The method of current existing human peripheral blood cell detection is to be detected using CTC cell cytometry, basic
Principle be by all cells by label processing one by one flow through detection zone, by fluorescence detection to rare cell or
Sick cell carries out detection counting.Be individual cells serial sensing method since it is used, detection cycle is very long, detection at
This is very high.
Using push jack technology, by whole blood cells, single layer is unfolded on slide, and is known using naked eyes identification or optical imagery
Other rare cell or sick cell, the screening of Lai Jinhang rapid cellular count, and are novel cell detection techniques.It is based on streaming
Counting method, speed greatly improve, and detection time is short, at low cost.
The push jack machine of clinical use includes the SC-120 type sheet pushing machine of the SP-1000i and Mai Rui of western gloomy Meikang, often
It is matched with blood routine, for detecting all kinds of white blood cell count(WBC)s in blood.60 μ L-200 μ of this two instrument push jack sample volume
L can accurately examine a large amount of existing cell of blood, such as eosinophil, lymphocyte, monocyte
It surveys, the preferable minute sites of push jack need to be only selected in clinical detection, identify the general ratio of pathogenic cell.
But this two, reason helpless for the detection of rare cell and trace pathogenic microorganism are as follows:
1) sample size is inadequate, this two instrument does not have continuously tonic apparatus module, can only to 60-200 μ L sample into
Row push jack, and rare cell and trace the pathogenic microorganism examination need in 1,000,000,000 peripheral bloods (about 7.5mL sample) billions of
Tens or even several rare cells and trace pathogenic microorganism are fast and accurately identified in cell.
2) push jack quality is inadequate.It is limited by application, the SC-120 of the SP-1000i and Mai Rui of western gloomy Meikang are for push jack
Head and tail portion do not require, and only need to have small cell monolayer segment in push jack middle section, head-tail is divided into more
Confluent monolayer cells are gathered together.And for fast and accurately knowing in billions of a cells in 1,000,000,000 peripheral bloods (7.5ml sample)
It Chu not be for tens or even several rare cells and trace pathogenic microorganism, it is clear that each section of entire push jack is required
It is single layer, to guarantee that rare cell will not be missed because cell is superimposed.
Summary of the invention
Aiming at the deficiencies in the prior art, this case proposes a kind of for making the pushing device of cell monolayer.
Technical solution of the present invention is summarized as follows:
It is a kind of for making the pushing device of cell monolayer comprising:
Substrate, two sides are equipped with guide rail bar；
Push jack, one end are used to contact with the guide rail bar, and will in a manner of slidable movement in the guide rail bar
Cell liquid tiles on the substrate；
Push jack folder, connect with the push jack；
First axle component comprising have first axle mounting base and first axle；The first axle component passes through institute
It states first axle and push jack folder realizes connection；
Second hinge component comprising have second hinge mounting base and second hinge；The second hinge component with it is described
The connection of first axle component；
Wherein, the direction of propulsion of the first axle and the second hinge spatially Relative vertical.
Preferably, the pushing device for being used to make cell monolayer, wherein the height of the guide rail bar is 5-20
μm。
Preferably, the pushing device for being used to make cell monolayer, wherein set in the first axle mounting base
It is equipped with the block piece for limiting the push jack folder rotation angle.
Preferably, the pushing device for being used to make cell monolayer, wherein also set up in the first axle
Flexible part, the elastic component generate screen resilience when the push jack is pressed from both sides and rotated.
Preferably, the pushing device for being used to make cell monolayer, wherein be also provided with use inside the push jack
In the runner of fluid injection.
Preferably, the pushing device for being used to make cell monolayer, wherein the push jack surface is also provided with use
In the groove of accommodating liquid injection pipe.
Preferably, the pushing device for being used to make cell monolayer, wherein the push jack surface is also provided with use
In the routing hole that liquid injection pipe interts.
Preferably, the pushing device for being used to make cell monolayer, wherein between the push jack and push jack folder also
Equipped with elastomeric pad.
The beneficial effects of the present invention are: the device that this case proposes infuses sample technology using continuous, and using on double freedom
Push jack posture automatic fine tuning technology, the quick continuous single layer that can be realized 1,000,000,000 cells (7.5mL blood sample) are spread out, and whole
The case where being not in multi-layer cellular superposition on a cellular layer.The pushing device can be cooperated with big visual field pattern imaging equipment
It uses, the individual rare cells and sick cell in 1,000,000,000 cells (7.5mL blood sample) can be quickly found out, for infectiousness
The detection of the early clinic of disease and tumor and cancer is of great significance.
Detailed description of the invention
Fig. 1 is the structural schematic diagram for making the pushing device of cell monolayer.
Fig. 2 is the structural schematic diagram of the pushing device of component containing first axle and second hinge component section.
Fig. 3 is the partial enlarged view of A in Fig. 2.
Fig. 4 is the structural schematic diagram that pushing device uses sample introduction fluid injection mode one.
Fig. 5 is the structural schematic diagram that pushing device uses sample introduction fluid injection mode two.
Fig. 6 is the structural schematic diagram of substrate in pushing device.
Dynamic schematic diagram when Fig. 7 is first axle and second hinge relative rotation spatially.
Fig. 8 is structural schematic diagram when multiple groups pushing device is combined simultaneously.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
As shown in figures 1-8, the pushing device for being used to make cell monolayer of an embodiment is listed in this case comprising:
Substrate 1, two sides are equipped with guide rail bar 2；
Push jack 3, one end for being contacted with guide rail bar 2, and in guide rail bar 2 in a manner of slidable movement by cell liquid
Tiling is on substrate 1；The effect of guide rail bar 2 is: 1) overcomes blood sample viscosity, while reducing the contact area of push jack 3 Yu substrate 1,
To reduce resistance when promoting on substrate 1；2) gap between push jack 3 and substrate 1 is controlled, to meet the flat of cell monolayer
Paving needs.
Push jack folder 4, connect with push jack 3；
First axle component comprising have first axle mounting base 5 and first axle 6；First axle component passes through first
Hinge 6 and push jack folder 4 are realized and are connected；
Second hinge component comprising have second hinge mounting base 7 and second hinge 8；Second hinge component and the first hinge
Chain component connection；
Wherein, the direction of propulsion of first axle 6 and second hinge 8 spatially Relative vertical (as shown in Figure 7).First hinge
Chain 6 and second hinge 8 are that can be realized the bindiny mechanism of relative rotation, including but not limited to the connection side of " axis-bearing " form
Formula.For first axle 6 for connecting push jack folder 4 and first axle mounting base 5, push jack folder 4 and first axle installation is may be implemented in it
Relative rotation between seat 5 along the x axis.Second hinge 8 is used to connect first axle mounting base 5 and second hinge mounting base 7,
The relative rotation between first axle mounting base 5 and second hinge mounting base 7 along the y axis may be implemented in it.6 He of first axle
Second hinge 8 makes push jack 3 have two rotational freedoms (as shown in Figure 7) in X, Y direction.
Wherein, the highly preferred of guide rail bar 2 is 5-20 μm, and the diameter of cell is able to guarantee in push jack at 5-20 μm
Cell is effectively protected in the process.
Wherein, the block piece 9 for limiting 4 rotation angle of push jack folder is provided in first axle mounting base 5.Block piece 9
The movement that 4 can be pressed from both sides to push jack carries out resistance and works as, and effect is carried out to the rotation angle between push jack 3 and first axle mounting base 5
Limitation, to guarantee in the moment that push jack 3 is in contact with substrate 1, without departing from one definite value of angle between the two (such as: 30
Degree), to protect push jack 3 itself and raising to be bulldozed quality.
Wherein, elastic component 10 is additionally provided in first axle 6, which generates rebound in push jack 4 rotation of folder
Power.Elastic force can be applied between push jack folder 4 and first axle mounting base 5 by elastic component 10, when push jack folder 4 is relative to first
When having relative rotation between hinge installation seat 5, the deformation of elastic component 10 allows to generate certain screen resilience, one side of this power
Face can press from both sides the angle between 4 and first axle mounting base 5 to push jack in a free state and constrain, and on the other hand push away
When piece 3 is in contact with substrate 1 and carries out push jack, so that the guide rail bar 2 that on push jack 3 there is certain pushing power to act on substrate 1
On, it is fitted closely always with guaranteeing during push jack push jack 3 with guide rail bar 2.Elastic component 10 can be with are as follows: torsionspring, with
One hinge 6 coaxial (such as Fig. 3), one end are supported on push jack folder 4, and the other end is supported in first axle mounting base 5；Or it stretches
Spring, one end are fixed on push jack folder 4, in other end fixation and first axle mounting base 5；Or compressed spring, one end are fixed
It is pressed from both sides on 4 in push jack, in other end fixation and first axle mounting base 5.
In addition, the present apparatus can also realize continuous sample introduction fluid injection, it can take various forms to realize:
Mode one: the runner 11 for fluid injection is also provided with inside push jack 3, such as Fig. 4.
Mode two: 3 surface of push jack is also provided with the groove 12 for accommodating liquid injection pipe.3 surface of push jack also preferably opens up
There is the routing hole 13 interted for liquid injection pipe, such as Fig. 5.
In addition, elastomeric pad 14 is additionally provided between push jack 3 and push jack folder 4, to realize the bullet between push jack 3 and push jack folder 4
Property connection, guarantee during the installation process push jack 3 will not damage.
Second hinge mounting base 7 and substrate 1 when in use can with external driving equipment, as linear motor or rotating electric machine+
The mode of screw rod is generated in this role in relative motion, including Z-direction and Y direction (on the basis of Fig. 7 fixed setting)
Translational motion.Driving equipment may include one or more linear motors or rotating electric machine+screw rod driving equipment.To improve
Flux and efficiency, this pushing device, which may include multiple main bodys, to be come while carrying out push jack operation, sees Fig. 8.
The realization of above-mentioned relative motion can be following methods:
A) substrate 1 is fixed, and second hinge mounting base 7 carries out the translation of Z, Y-direction under the driving of external driving equipment
Movement.
B) substrate 1 carries out Y-direction translational motion in the driving system of external driving equipment, and second hinge mounting base 7 is external
The translational motion of Z-direction is carried out under the driving of driving equipment.
C) substrate 1 carries out Z, the translational motion in Y-direction under the driving of external driving equipment, and second hinge mounting base
7 are kept fixed.
The basic operational process of this pushing device is as follows:
1) substrate 1 is placed；
2) under the driving of external driving device, push jack 3 slowly moves and moves closer to base in the perpendicular direction in substrate 1
Plate 1；
3) in push jack 3 and 1 contact process of substrate, first axle 6 and second hinge 8 will be according between push jack 3 and substrates 1
Contact force, automatic fine tuning push jack state fits closely the sideline of push jack 3 with substrate 1；
4) continue push jack 3 under the driving of external driving device to continue to move towards the direction perpendicular to substrate 1, it is elastic at this time
Part 10 starts to act on, and push jack 3 is made on substrate 1, to guarantee the close contact during push jack with certain pressure effect；
5) sample is slowly injected into note sample runner by external connection power source.Sample is scattered in substrate under the action of capillary force
On 1 sideline.Meanwhile push jack 3 is translated along 1 length direction of substrate under the driving of external driving device, and sample is continuous
Uniform single berth is opened on substrate 1；
6) push jack finishes, and push jack 3 is first done vertically with 1 direction of substrate far from substrate 1 under the driving of external driving device, after do
It is parallel to 1 direction translational of substrate, it is automatic to restore to initial position, while external connection power source cleaning note sample runner, to guarantee without friendship
Fork pollution.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details.
Claims (7)
1. a kind of for making the pushing device of cell monolayer characterized by comprising
Substrate, two sides are equipped with guide rail bar；
Push jack, one end for being contacted with the guide rail bar, and in the guide rail bar in a manner of slidable movement by cell
Liquid tiles on the substrate；
Push jack folder, connect with the push jack；
First axle component comprising have first axle mounting base and first axle；The first axle component passes through described the
One hinge and push jack folder realize connection；
Second hinge component comprising have second hinge mounting base and second hinge；The second hinge component and described first
Hinge component connection；
Wherein, the direction of propulsion of the first axle and the second hinge spatially Relative vertical；
Wherein, the height of the guide rail bar is 5-20 μm.
2. according to claim 1 for making the pushing device of cell monolayer, which is characterized in that the first axle peace
The block piece for limiting the push jack folder rotation angle is provided on dress seat.
3. according to claim 1 for making the pushing device of cell monolayer, which is characterized in that in the first axle
On be additionally provided with elastic component, the elastic component the push jack press from both sides rotate when generate screen resilience.
4. according to claim 1 for making the pushing device of cell monolayer, which is characterized in that inside the push jack also
Offer the runner for fluid injection.
5. according to claim 1 for making the pushing device of cell monolayer, which is characterized in that the push jack surface is also
Offer the groove for accommodating liquid injection pipe.
6. according to claim 5 for making the pushing device of cell monolayer, which is characterized in that the push jack surface is also
Offer the routing hole interted for liquid injection pipe.
7. according to claim 1 for making the pushing device of cell monolayer, which is characterized in that the push jack and push jack
Elastomeric pad is additionally provided between folder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611167340.9A CN106479863B (en) | 2016-12-16 | 2016-12-16 | For making the pushing device of cell monolayer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611167340.9A CN106479863B (en) | 2016-12-16 | 2016-12-16 | For making the pushing device of cell monolayer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106479863A CN106479863A (en) | 2017-03-08 |
| CN106479863B true CN106479863B (en) | 2019-08-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611167340.9A Active CN106479863B (en) | 2016-12-16 | 2016-12-16 | For making the pushing device of cell monolayer |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6110425A (en) * | 1998-12-03 | 2000-08-29 | Coulter International Corp. | Blood smear slide outloader |
| CN2855326Y (en) * | 2005-12-21 | 2007-01-10 | 深圳迈瑞生物医疗电子股份有限公司 | Preparator of blood smear |
| CN201289540Y (en) * | 2008-11-14 | 2009-08-12 | 王志效 | Novel glass carrier |
| CN101694431A (en) * | 2009-10-15 | 2010-04-14 | 北京赛尔蒂扶科技发展有限公司 | Automatic peripheral blood pushing device |
| CN203365204U (en) * | 2013-08-10 | 2013-12-25 | 殷盈祥 | Slide coating device for blood inspection |
| CN204594769U (en) * | 2015-04-28 | 2015-08-26 | 范琳 | Convenient blood sample smear film-making platform |
| CN105300759A (en) * | 2015-09-30 | 2016-02-03 | 爱威科技股份有限公司 | Scraper fixing device and blood smear pushing device |
| CN206337256U (en) * | 2016-12-16 | 2017-07-18 | 中国科学院苏州生物医学工程技术研究所 | Pushing device for making cell monolayer |
-
2016
- 2016-12-16 CN CN201611167340.9A patent/CN106479863B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6110425A (en) * | 1998-12-03 | 2000-08-29 | Coulter International Corp. | Blood smear slide outloader |
| CN2855326Y (en) * | 2005-12-21 | 2007-01-10 | 深圳迈瑞生物医疗电子股份有限公司 | Preparator of blood smear |
| CN201289540Y (en) * | 2008-11-14 | 2009-08-12 | 王志效 | Novel glass carrier |
| CN101694431A (en) * | 2009-10-15 | 2010-04-14 | 北京赛尔蒂扶科技发展有限公司 | Automatic peripheral blood pushing device |
| CN203365204U (en) * | 2013-08-10 | 2013-12-25 | 殷盈祥 | Slide coating device for blood inspection |
| CN204594769U (en) * | 2015-04-28 | 2015-08-26 | 范琳 | Convenient blood sample smear film-making platform |
| CN105300759A (en) * | 2015-09-30 | 2016-02-03 | 爱威科技股份有限公司 | Scraper fixing device and blood smear pushing device |
| CN206337256U (en) * | 2016-12-16 | 2017-07-18 | 中国科学院苏州生物医学工程技术研究所 | Pushing device for making cell monolayer |
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| CN106479863A (en) | 2017-03-08 |
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