CN1064708C - Composite juice of diplobacillus ferment carrot and making method - Google Patents
Composite juice of diplobacillus ferment carrot and making method Download PDFInfo
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- CN1064708C CN1064708C CN95102893A CN95102893A CN1064708C CN 1064708 C CN1064708 C CN 1064708C CN 95102893 A CN95102893 A CN 95102893A CN 95102893 A CN95102893 A CN 95102893A CN 1064708 C CN1064708 C CN 1064708C
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- bifidus bacillus
- bacterium
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- carrot
- milk
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Abstract
The present invention belongs to a processing field of food and beverages. Bifidobacteria separated from longevous people are screened to obtain bacterial strains with good characters, wherein the bacterial strains are suitable for the fermentation of beverages, such as vegetable juice, etc. and are applied to the fermentation of compound carrot juice, and the compound juice is reasonably prepared from carrot juice, skimmed milk and adjuvant materials. The fermented juice contains a large amount of live bifidobacteria, rich nutrients, such as beta-carotene, etc. and various nutrients, such as metabolic products of the bifidobacteria, such as beta family vitamins, acetic acid, lactic acid, amino acids, etc. The product has the advantages of bright color and good flavor, and is a good nutritive health care beverage for various people, especially persons in middle and old age.
Description
The present invention relates to a kind of bifidobacterium strains and with this strain fermentation carrot compound juice beverage and manufacture method thereof.
Kind surplus the product of external bifidus bacillus has reached 70, they comprise yogurt, buttermilk, yogurt fat, milk powder, quick-frozen dessert, tablet and lozenge etc.Domestic applied bifidus bacillus is adopted the bacterial classification that separation obtains from infant faeces more, and to healthy old men, particularly the bifidus bacillus that comes from long-lived crowd is studied seldom, and the application in juice beverage still is not reported.
Purpose of the present invention is separated bifidus bacillus exactly from the world's the 5th Changshou District-Guangxi crust horse long lived elder, therefrom screening has the bacterial strain of good character, moderate, the synthetic more bacterial strain of vitamin B group of acid can be grown fast, be produced to selection in carrot compound juice, and with this strain fermentation trailing plants compound juice and other fermented drink recklessly, and solve its fermentation process.
The present invention includes following steps:
1. a separation is from the bifidus bacillus (Bifidobacterium) of long lived elder, bacterial classification is bifidobacterium adolescentis (B.aldolescentis), bacterium numbering is BAU-B0330, preserving number is: CGMCCNO.0223.1 or bifidus longum bb (B.longum), bacterium numbering is BAU-B1024, preserving number is: CGMCC NO.0223.2 or bifidumbacterium bifidum (B.bifidum), bacterium numbering is BAU-B0111, preserving number is: CGMCC NO.0223.3 or bifidobacteria infantis (B.infantis), bacterium numbering is BAU-B007, and preserving number is: CGMCC NO.0223.4.
2. a bifidus bacillus carrot compound juice contains bifidus bacillus, Radix Dauci Sativae, skimmed milk or degreasing reconstituted milk or soya-bean milk, sugar and salt.
3. bifidus bacillus carrot compound juice, the weight ratio of various compositions is carrot paste 50-95%.Degreasing fresh cow milk or degreasing reconstituted milk or soya-bean milk 5-50% (the degreasing reconstituted milk: skim-milk is 1: 7 with the ratio of water) are 100% calculating with above-mentioned compound juice, add sugared 2-7%, or add other sweeting agent, and salt 1-3%, bifidus bacillus content are 10
7-10
9Individual/ml.
4. the preparation technology of a bifidus bacillus carrot compound juice, it comprises separation, screening, the carrot compound juice preparation of bacterial classification:
A: the cultivation of bifidus bacillus
The inoculation of employing liquid nutrient medium is transferred 2-3 time continuously, and the bacterium number reaches 10 in the seed liquor
8-10
9/ ml.When being used for ferment-seeded (bacterial classification), enlarge step by step according to the needs of fermentation base-material amount, last enlarged culturing based formulas is identical with the base-material that ferments.All the other nutrient solutions adopt conventional bifidus bacillus substratum (BL substratum, GAM, modified MRS).The seed liquor index: do not have assorted bacterium, pH is 4.2-4.5, bacterium several 10
8-10
9Individual/ml.
B: bifidus bacillus carrot compound juice zymotechnique
The carrot compound juice that homogeneous is good pumps in the bio-reactor, is heated to 115 ℃ with jacket steam, sterilizes 15-20 minute, is cooled to 40 ℃ rapidly, inserts bifidus bacillus seed liquid, and inoculum size is calculated as 10 by the bacterium number
5-10
6Individual/ml (volume ratio is 0.2-2%) fed aseptic nitrogen heat-preservation fermentation 10-18 hour, when the bacterium number reaches 10
7-10
9Individual/ml, fermentation ends during pH4.0-4.5 is cooled to rapidly below 20 ℃, and sterile filling in the clean room of cleanliness factor 100-1000 level needs storing below 10 ℃ for keeping high viable count, or adds bifid bacterium microcapsule.
Advantage of the present invention
The present invention is an isolating bifidus bacillus from long lived elder, selects the bacterial strain with good character, is used for the ferment carrot compound juice, and this bifidus bacillus is easier to adapt to field planting in middle-aged and old enteron aisles easily to middle-aged and old digestive tract environment.The beneficial effect of bifidus bacillus generally acknowledged by common people, but the used bacterial classification of product on the market all separates from the baby now, and the elderly is not exclusively adapted to, and therefore product of the present invention is more suitable in the elderly.Along with China moves towards aging society gradually, the elderly's health care is outstanding day by day, so product of the present invention will have vast market.Except that the effect of bifidus bacillus, having selected the Radix Dauci Sativae of being rich in β-Hu Luobusu in the present invention for use is raw material.Match with nourishing milk, the fermentation of process bifidus bacillus has increased B group vitamin contents.Also having more acetate and lactic acid in the fermented juice, to helping digest, suppress enteron aisle spoilage organism and pathogenic bacterium good effect is arranged, make product have higher health-care effect and nutritive value, is that other beverage all can't be compared on the market.
Product has bright-coloured color and luster and good local flavor, can be accepted by most of people, and raw materials cost is lower in addition, and therefore market outlook and economic benefit are preferably arranged.
The present invention has following accompanying drawing figure l fermented carrot juice using bifidobacteria and manufacturing approach craft schema thereof
Most preferred embodiment of the present invention
1. bifidus bacillus separates, cultivates embodiment:
A. bifidus bacillus (Bifidobacterium), bifidobacterium adolescentis (B.aldolescentis), be numbered: BAU-B0330, preserving number is: CGMCC NO.0223.1 or bifidus longum bb (B.longum), be numbered: BAU-B1024, preserving number is: CGMCC NO.0223.2 or bifidumbacterium bifidum (B.bifidum), be numbered: BAU-B0111, preserving number is: CGMCC NO.0223.3 or bifidobacteria infantis (B.infantis), be numbered: BAU-B007, preserving number is: CGMCC NO.0223.4.Depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center, preservation date: March 16 nineteen ninety-five.
B. the separation of bifidus bacillus: get good health and a long life old man fresh excreta, under anaerobic separation method separates routinely, obtains single bacterium colony, is further purified, and belongs to according to uncle's Jie Shi bacteriology handbook (1986) then and plants evaluation, obtains above-mentioned bacterial strains.
C. bacterial screening: the purified and bifidus bacillus of identifying of inoculation in compound Radix Dauci Sativae juice, cultivate 24h down at 37 ℃, measure bacterium number, pH value, Titrable acid and vitamin B group (B1, B2, B6, B12), taste the fermented liquid local flavor simultaneously, compare, select the best performance bacterial strain and use for fermentative production.
D. spawn culture: get a ring or 0.2ml is seeded to (BL or MRS add bifidus factor) the anaerobism test tube that the 10ml liquid nutrient medium is housed from preservation bacterial classification test tube, in 37 ℃ of constant temperature culture 24h, taking out 0.2ml then inserts in second anaerobism pipe, cultivate 18h, get 0.2ml and insert the 3rd anaerobism pipe and cultivate 18h, get in the anaerobism bottle that 1ml inserts the compound Radix Dauci Sativae juice that the 50ml sterilization is housed, cultivate 18h, microscopy does not have assorted bacterium, measures the bifidus bacillus number and reaches bacterium several 10
8-10
9Individual/ml, pH is 4.2-4.5, cools off standby.
2. product embodiments:
Product is by bifidus bacillus, carrot compound juice, skimmed milk or degreasing reconstituted milk (or replace cow's milk with soya-bean milk), sweeting agent and salt.Its formula rate is: contain bifidus bacillus 10 in the product
6-10
9Individual/ml, Radix Dauci Sativae juice 50-95%, degreasing fresh cow milk or degreasing reconstituted milk (or soya-bean milk) 5-50%, totally 100%, add the 2-7% sucrose (or add other sweeting agent, ratio is allocated according to taste) of above-mentioned total amount, salt 1-2% on this basis.
3. carrot compound juice complete processing embodiment:
Remove the Radix Dauci Sativae 1000g behind the skin, be cut into the thick sheet of 2-3mm, put into 2000g95-100 ℃ of hot water, keep 95-100 ℃ temperature 5-10 minute, connect water band carrot slice making beating (available homogenizer or hollander) then, use colloidal mill homogeneous 3-10 minute, add the 5-10% skimmed milk, the salt of 3-4% sucrose and 1-2% makes it to become even and not stratified compound juice after colloidal mill or clarifixator.Add pine for noting following some:
1). Radix Dauci Sativae need be selected redness, fine and tender taste, kind that fiber is few.
2). peeling can be adopted artificial or the peeling of thermokalite water.
3). heat iron temperature is unsuitable too high, and the time is unsuitable long, minute is advisable with 90-100 ℃, 3-10, otherwise ＂ cooked taste ＂ will be arranged.
4). homogenization pressure and time should reach the requirement of carrot cell fragmentation within a short period of time, and general homogenization pressure is 150-250kg/cm
2, 15-30 minute.
4. carrot compound juice zymotechnique embodiment:
The good compound juice of homogeneous is put into small-sized glass biological reaction device,, be cooled to 40 ℃ rapidly through 115 ℃ of sterilizations in 15 minutes.
Get the seed liquor 6-30ml for preparing, insert in the bio-reactor under aseptic technique, stir, feed aseptic nitrogen and cause anaerobic condition, at 36-38 ℃ of temperature bottom fermentation, every 2h sampling and measuring pH and bacterium number are when the bacterium number reaches 10
8-10
9Individual/ml, stop fermentation during pH4.2-4.5, cool, under aseptic condition, pour in the vial of sterilization, promptly obtain product.
Because product is without sterilization, product is nutritious, prevents that living contaminants is very important, therefore will take strict sterile filling.In time send the freezer preservation after the can.For keeping high viable count, can add bifid bacterium microcapsule.
Claims (4)
1. a separation is from the bifidus bacillus (Bifidobacterium) of long lived elder, it is characterized in that bacterial classification is bifidobacterium adolescentis (B.aldolescentis), bacterium numbering is BAU-B0330, preserving number is CGMCC NO.0223.1 or bifidus longum bb (B.longum), bacterium numbering is BAU-B1024, preserving number is CGMCC NO.0223.2 or bifidumbacterium bifidum (B.bifidum), bacterium numbering is BAU-B0111, preserving number is NO.0223.3 or bifidobacteria infantis (B.infantis). be numbered BAU-B007, preserving number is CGMCC NO.0223.4.
2. a bifidus bacillus carrot compound juice is characterized in that containing one of described bifidus bacillus of claim 1, Radix Dauci Sativae, skimmed milk or degreasing reconstituted milk or soya-bean milk, sugar and salt.
3. bifidus bacillus carrot compound juice according to claim 2, it is characterized in that: the weight ratio of various compositions is carrot paste 50-95%, degreasing fresh cow milk or degreasing reconstituted milk or soya-bean milk 5-50% (the degreasing reconstituted milk: skim-milk is 1: 7 with the ratio of water), with above-mentioned compound juice is 100% calculating, add sugared 2-7%, or add other sweeting agent, and salt 1-3%, the described bifidus bacillus content of claim 1 is 10
7-10
9Individual/ml.
4. the preparation technology of a bifidus bacillus carrot compound juice, it comprises separation, screening, the carrot compound juice preparation of bacterial classification, it is characterized in that:
A: claim 1 is cultivated by described bifidus bacillus
The inoculation of employing liquid nutrient medium is transferred 2-3 time continuously, and the bacterium number reaches 10 in the seed liquor
8-10
9Individual/ml, when being used for ferment-seeded (bacterial classification), enlarge step by step according to the needs of fermentation base-material amount, last enlarged culturing based formulas is identical with the fermentation base-material, and all the other nutrient solutions adopt conventional bifidus bacillus substratum, the seed liquor index: do not have assorted bacterium, pH is 4.2-4.5, bacterium several 10
8-10
9Individual/ml.
B: bifidus bacillus carrot compound juice zymotechnique
The carrot compound juice that homogeneous is good pumps in the bio-reactor, is heated to 115 ℃, sterilizes 15-20 minute, is cooled to 40 ℃ rapidly, inserts above-mentioned bifidus bacillus seed liquid, and inoculum size is calculated as 10 by the bacterium number
5-10
6Individual/ml (volume ratio is 0.2-2%) fed aseptic nitrogen heat-preservation fermentation 10-18 hour, when the bacterium number reaches 10
7-10
9Individual/ml, fermentation ends during pH4.0-4.5 is cooled to rapidly below 20 ℃, and sterile filling in the clean room of cleanliness factor 100-1000 level needs for keeping high viable count, is storing below 10 ℃, or is adding bifid bacterium microcapsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95102893A CN1064708C (en) | 1995-03-22 | 1995-03-22 | Composite juice of diplobacillus ferment carrot and making method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95102893A CN1064708C (en) | 1995-03-22 | 1995-03-22 | Composite juice of diplobacillus ferment carrot and making method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1116237A CN1116237A (en) | 1996-02-07 |
| CN1064708C true CN1064708C (en) | 2001-04-18 |
Family
ID=5074511
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95102893A Expired - Fee Related CN1064708C (en) | 1995-03-22 | 1995-03-22 | Composite juice of diplobacillus ferment carrot and making method |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1092520C (en) * | 1996-09-19 | 2002-10-16 | 段卫平 | Ecological enema liquid for curing functional constipation |
| CN101785516B (en) * | 2010-01-26 | 2012-05-23 | 东北农业大学 | Lactic acid bacterium fermentation fruit tea and preparation method thereof |
| CN102657261B (en) * | 2012-05-22 | 2014-01-08 | 光明乳业股份有限公司 | Carrot-containing yogurt and method for producing same |
| CN103275893A (en) * | 2013-05-06 | 2013-09-04 | 哈尔滨美华生物技术股份有限公司 | Complex microbial inoculants of bifidobacterium longum and bifidobacterium animalis and preparation method of complex microbial inoculants |
| CN107889994A (en) * | 2017-12-19 | 2018-04-10 | 南达新农业股份有限公司 | Fermentation type active lactic acid bacteria carrot juice beverage and preparation method thereof |
| CN109363021A (en) * | 2018-10-16 | 2019-02-22 | 宾川县华侨庄园农业科技开发有限公司 | A kind of processing method of orange blossom |
| CN111165710A (en) * | 2020-03-04 | 2020-05-19 | 任彦芬 | Bifidobacterium bifidum probiotic solid beverage with cell aging resistance |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1065397A (en) * | 1991-03-23 | 1992-10-21 | 董宝瑶 | A kind of preparation method of microbial nutrient solution |
-
1995
- 1995-03-22 CN CN95102893A patent/CN1064708C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1065397A (en) * | 1991-03-23 | 1992-10-21 | 董宝瑶 | A kind of preparation method of microbial nutrient solution |
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| CN1116237A (en) | 1996-02-07 |
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