CN106434856A - 一种测序仪运行测试的方法 - Google Patents
一种测序仪运行测试的方法 Download PDFInfo
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- CN106434856A CN106434856A CN201510477346.5A CN201510477346A CN106434856A CN 106434856 A CN106434856 A CN 106434856A CN 201510477346 A CN201510477346 A CN 201510477346A CN 106434856 A CN106434856 A CN 106434856A
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- 238000012360 testing method Methods 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000012163 sequencing technique Methods 0.000 claims description 88
- 238000009396 hybridization Methods 0.000 claims description 5
- 230000006698 induction Effects 0.000 claims description 5
- JNRLEMMIVRBKJE-UHFFFAOYSA-N 4,4'-Methylenebis(N,N-dimethylaniline) Chemical compound C1=CC(N(C)C)=CC=C1CC1=CC=C(N(C)C)C=C1 JNRLEMMIVRBKJE-UHFFFAOYSA-N 0.000 claims description 2
- 108020004414 DNA Proteins 0.000 description 28
- 238000006243 chemical reaction Methods 0.000 description 8
- 239000012634 fragment Substances 0.000 description 7
- 238000000746 purification Methods 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 4
- 102000003960 Ligases Human genes 0.000 description 4
- 108090000364 Ligases Proteins 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 239000000376 reactant Substances 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000013467 fragmentation Methods 0.000 description 3
- 238000006062 fragmentation reaction Methods 0.000 description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 235000011147 magnesium chloride Nutrition 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000002493 microarray Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 239000013611 chromosomal DNA Substances 0.000 description 2
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- 125000004122 cyclic group Chemical group 0.000 description 2
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- 239000002077 nanosphere Substances 0.000 description 2
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- 238000003752 polymerase chain reaction Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- YTBSYETUWUMLBZ-QWWZWVQMSA-N D-threose Chemical compound OC[C@@H](O)[C@H](O)C=O YTBSYETUWUMLBZ-QWWZWVQMSA-N 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
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- 230000004544 DNA amplification Effects 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
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- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 description 1
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- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
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- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 230000000865 phosphorylative effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
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- 238000010998 test method Methods 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
编号 | 序列(5’-3’) | Seq ID No. |
1 | TTAATTAAGG | 5 |
2 | GACTCACTGA | 6 |
3 | ATAAGGCAGT | 7 |
4 | AATATCTCTC | 8 |
编号 | 序列(5’-3’) | Seq ID No. |
1 | ACTGCTGACGTACTGTTAATTAAG | 9 |
2 | ACTGCTGACGTACTGGACTCACTG | 10 |
3 | ACTGCTGACGTACTGATAAGGCAG | 11 |
4 | ACTGCTGACGTACTGAATATCTCT | 12 |
Claims (6)
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CN201510477346.5A CN106434856B (zh) | 2015-08-06 | 2015-08-06 | 一种测序仪运行测试的方法 |
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CN106434856A true CN106434856A (zh) | 2017-02-22 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109207471A (zh) * | 2017-06-30 | 2019-01-15 | 深圳华大基因股份有限公司 | 一种构建分片段核酸文库的方法及其应用 |
WO2020037571A1 (zh) * | 2018-08-22 | 2020-02-27 | 深圳市真迈生物科技有限公司 | 基于图像构建测序模板的方法、装置和计算机程序产品 |
CN112289381A (zh) * | 2018-08-22 | 2021-01-29 | 深圳市真迈生物科技有限公司 | 基于图像构建测序模板的方法、装置和计算机程序产品 |
WO2024138344A1 (zh) * | 2022-12-26 | 2024-07-04 | 青岛华大智造科技有限责任公司 | 基因测序方法、***、电子设备和存储介质 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101415839A (zh) * | 2006-02-08 | 2009-04-22 | 亿明达剑桥有限公司 | 对多核苷酸模板进行测序的方法 |
CN104293940A (zh) * | 2014-09-30 | 2015-01-21 | 天津华大基因科技有限公司 | 构建测序文库的方法及其应用 |
-
2015
- 2015-08-06 CN CN201510477346.5A patent/CN106434856B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101415839A (zh) * | 2006-02-08 | 2009-04-22 | 亿明达剑桥有限公司 | 对多核苷酸模板进行测序的方法 |
CN104293940A (zh) * | 2014-09-30 | 2015-01-21 | 天津华大基因科技有限公司 | 构建测序文库的方法及其应用 |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109207471A (zh) * | 2017-06-30 | 2019-01-15 | 深圳华大基因股份有限公司 | 一种构建分片段核酸文库的方法及其应用 |
WO2020037571A1 (zh) * | 2018-08-22 | 2020-02-27 | 深圳市真迈生物科技有限公司 | 基于图像构建测序模板的方法、装置和计算机程序产品 |
CN112289381A (zh) * | 2018-08-22 | 2021-01-29 | 深圳市真迈生物科技有限公司 | 基于图像构建测序模板的方法、装置和计算机程序产品 |
CN112289381B (zh) * | 2018-08-22 | 2021-12-14 | 深圳市真迈生物科技有限公司 | 基于图像构建测序模板的方法、装置和计算机产品 |
WO2024138344A1 (zh) * | 2022-12-26 | 2024-07-04 | 青岛华大智造科技有限责任公司 | 基因测序方法、***、电子设备和存储介质 |
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CN106434856B (zh) | 2020-02-07 |
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Address after: Beishan Industrial Zone Building in Yantian District of Shenzhen city of Guangdong Province in 518083 Applicant after: Shenzhen Huada Academy of life science Applicant after: BGI-Shenzhen Co., Ltd. Address before: 518083, Beishan Industrial Zone, 146 North Mountain Road, Yantian District, Shenzhen, Guangdong Applicant before: BGI-Shenzhen Applicant before: BGI-Shenzhen Co., Ltd. |
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CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Feng Hetian Inventor after: Zhang Yongwei Inventor after: Ni Ming Inventor after: Zhang Wenwei Inventor after: Dai Chongchong Inventor after: Chen Ao Inventor after: Wang Jingjing Inventor after: Luo Yufen Inventor after: Luo Yinling Inventor before: Feng Hetian Inventor before: Zhang Yongwei Inventor before: Ni Ming Inventor before: Dai Chongchong Inventor before: Chen Ao Inventor before: Wang Jingjing Inventor before: Luo Yufen Inventor before: Luo Yinling |
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Effective date of registration: 20180523 Address after: 518083 the comprehensive building of Beishan industrial zone and 11 2 buildings in Yantian District, Shenzhen, Guangdong. Applicant after: Shenzhen Hua made Dazhi Technology Co. Ltd. Address before: 518083 comprehensive building, Beishan Industrial Zone, Yantian District, Shenzhen, Guangdong Applicant before: Shenzhen Huada Academy of life science Applicant before: BGI-Shenzhen Co., Ltd. |
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CP01 | Change in the name or title of a patent holder | ||
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Address after: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Patentee after: Shenzhen Huada Zhizao Technology Co., Ltd Address before: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Patentee before: Shenzhen Huada Zhizao Technology Co.,Ltd. |