CN106434840A - 一种用于微生物法定量检测维生素b12的微孔板、其试剂盒及其制备方法 - Google Patents
一种用于微生物法定量检测维生素b12的微孔板、其试剂盒及其制备方法 Download PDFInfo
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- CN106434840A CN106434840A CN201611094901.7A CN201611094901A CN106434840A CN 106434840 A CN106434840 A CN 106434840A CN 201611094901 A CN201611094901 A CN 201611094901A CN 106434840 A CN106434840 A CN 106434840A
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- microwell plate
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- bacterium solution
- trehalose
- vitamin
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Links
- 229930003779 Vitamin B12 Natural products 0.000 title claims abstract description 39
- 239000011715 vitamin B12 Substances 0.000 title claims abstract description 39
- 235000019163 vitamin B12 Nutrition 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 title claims abstract 11
- 238000013048 microbiological method Methods 0.000 title abstract 2
- 241000894006 Bacteria Species 0.000 claims abstract description 57
- 238000000034 method Methods 0.000 claims abstract description 39
- 239000001110 calcium chloride Substances 0.000 claims abstract description 27
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 27
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 26
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 26
- 229930006000 Sucrose Natural products 0.000 claims abstract description 26
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 26
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 26
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 25
- 238000001514 detection method Methods 0.000 claims abstract description 25
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- 241000186660 Lactobacillus Species 0.000 claims description 51
- 239000005720 sucrose Substances 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 230000004913 activation Effects 0.000 claims description 18
- 239000008267 milk Substances 0.000 claims description 18
- 235000013336 milk Nutrition 0.000 claims description 18
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- 230000000813 microbial effect Effects 0.000 claims description 16
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- 239000000843 powder Substances 0.000 claims description 14
- 230000001580 bacterial effect Effects 0.000 claims description 13
- 239000012530 fluid Substances 0.000 claims description 13
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 9
- 238000007710 freezing Methods 0.000 claims description 8
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- 238000004821 distillation Methods 0.000 claims description 7
- 239000012153 distilled water Substances 0.000 claims description 7
- 238000001704 evaporation Methods 0.000 claims description 7
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- 238000005119 centrifugation Methods 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 229940099596 manganese sulfate Drugs 0.000 claims description 5
- 239000011702 manganese sulphate Substances 0.000 claims description 5
- 235000007079 manganese sulphate Nutrition 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 claims description 5
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 5
- 229940068968 polysorbate 80 Drugs 0.000 claims description 5
- 229920000053 polysorbate 80 Polymers 0.000 claims description 5
- 239000012138 yeast extract Substances 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- 235000015278 beef Nutrition 0.000 claims description 4
- 239000000284 extract Substances 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 239000001632 sodium acetate Substances 0.000 claims description 4
- 235000017281 sodium acetate Nutrition 0.000 claims description 4
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 3
- 239000003223 protective agent Substances 0.000 abstract description 8
- 239000007788 liquid Substances 0.000 abstract description 5
- 238000004108 freeze drying Methods 0.000 abstract description 4
- 241000186673 Lactobacillus delbrueckii Species 0.000 abstract 1
- 235000013681 dietary sucrose Nutrition 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 29
- 239000000243 solution Substances 0.000 description 28
- 239000012925 reference material Substances 0.000 description 9
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 235000011194 food seasoning agent Nutrition 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 150000003722 vitamin derivatives Chemical class 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- 244000131522 Citrus pyriformis Species 0.000 description 2
- 241001134654 Lactobacillus leichmannii Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000003149 assay kit Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 230000005059 dormancy Effects 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 238000009533 lab test Methods 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 230000007483 microbial process Effects 0.000 description 2
- 238000011022 operating instruction Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 238000009631 Broth culture Methods 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- BDKZHNJTLHOSDW-UHFFFAOYSA-N [Na].CC(O)=O Chemical compound [Na].CC(O)=O BDKZHNJTLHOSDW-UHFFFAOYSA-N 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
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- 229940039696 lactobacillus Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
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CN201611094901.7A CN106434840B (zh) | 2016-12-02 | 2016-12-02 | 一种用于微生物法定量检测维生素b12的微孔板、其试剂盒及其制备方法 |
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CN201611094901.7A CN106434840B (zh) | 2016-12-02 | 2016-12-02 | 一种用于微生物法定量检测维生素b12的微孔板、其试剂盒及其制备方法 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109929901A (zh) * | 2019-03-13 | 2019-06-25 | 深圳容金科技有限公司 | 一种检测乳品中维生素b9含量的试剂盒及其制备方法 |
CN111621542A (zh) * | 2020-06-15 | 2020-09-04 | 深圳市瑞赛生物技术有限公司 | 一种即用型维生素b12检测载体及其制备方法 |
CN111705101A (zh) * | 2020-06-15 | 2020-09-25 | 深圳市瑞赛生物技术有限公司 | 一种即用型微量成分检测试剂盒及其制备方法 |
CN111705102A (zh) * | 2020-06-15 | 2020-09-25 | 深圳市瑞赛生物技术有限公司 | 一种即用型叶酸检测载体及其制备方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1193908A (zh) * | 1995-06-07 | 1998-09-23 | 廓德伦特控股剑桥有限公司 | 将物质稳定掺入干燥泡沫玻璃基质中的方法以及由此制得的组合物 |
CN1226842A (zh) * | 1996-07-15 | 1999-08-25 | 环球保藏技术股份有限公司 | 通过泡沫形成来保存的方法 |
US20080044846A1 (en) * | 2004-07-30 | 2008-02-21 | Ifp Privates Institut Fur Produktqualitat Gmbh | Method and Kit for the Microbiological Determination of Vitamins in Substance Mixtures |
-
2016
- 2016-12-02 CN CN201611094901.7A patent/CN106434840B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1193908A (zh) * | 1995-06-07 | 1998-09-23 | 廓德伦特控股剑桥有限公司 | 将物质稳定掺入干燥泡沫玻璃基质中的方法以及由此制得的组合物 |
CN1226842A (zh) * | 1996-07-15 | 1999-08-25 | 环球保藏技术股份有限公司 | 通过泡沫形成来保存的方法 |
US20080044846A1 (en) * | 2004-07-30 | 2008-02-21 | Ifp Privates Institut Fur Produktqualitat Gmbh | Method and Kit for the Microbiological Determination of Vitamins in Substance Mixtures |
Non-Patent Citations (1)
Title |
---|
黄晓林等: "IFP微孔板试剂盒检测配方乳粉中维生素B12方法探讨", 《中国乳品工业》 * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109929901A (zh) * | 2019-03-13 | 2019-06-25 | 深圳容金科技有限公司 | 一种检测乳品中维生素b9含量的试剂盒及其制备方法 |
CN111621542A (zh) * | 2020-06-15 | 2020-09-04 | 深圳市瑞赛生物技术有限公司 | 一种即用型维生素b12检测载体及其制备方法 |
CN111705101A (zh) * | 2020-06-15 | 2020-09-25 | 深圳市瑞赛生物技术有限公司 | 一种即用型微量成分检测试剂盒及其制备方法 |
CN111705102A (zh) * | 2020-06-15 | 2020-09-25 | 深圳市瑞赛生物技术有限公司 | 一种即用型叶酸检测载体及其制备方法 |
CN111705101B (zh) * | 2020-06-15 | 2021-08-24 | 深圳市瑞赛生物技术有限公司 | 一种即用型微量成分检测试剂盒及其制备方法 |
CN111621542B (zh) * | 2020-06-15 | 2023-08-22 | 深圳市瑞赛生物技术有限公司 | 一种即用型维生素b12检测载体及其制备方法 |
CN111705102B (zh) * | 2020-06-15 | 2023-09-12 | 深圳市瑞赛生物技术有限公司 | 一种即用型叶酸检测载体及其制备方法 |
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Effective date of registration: 20240604 Address after: Room 705, Building 3, No. 88 Kechuang 6th Street, Beijing Economic and Technological Development Zone, Daxing District, Beijing 102600 Patentee after: BEIJING ZHONGJIAN BAOTAI BIOTECHNOLOGY Co.,Ltd. Country or region after: China Address before: No. 32, Group 3, 17th Committee, A District, Mudanjiang Farmland Community, Mishan City, Jixi City, Heilongjiang Province, 158300 Patentee before: Chen Shuying Country or region before: China Patentee before: Wang Xinjian |
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