CN106434755A - Inonotus obliquus submerged fermentation product and application - Google Patents
Inonotus obliquus submerged fermentation product and application Download PDFInfo
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Abstract
The invention discloses an inonotus obliquus submerged fermentation product and application. The inonotus obliquus submerged fermentation product comprises a composition A, a composition B and a composition C. By the utilization of a biological fermentation technology, inonotus obliquus strains are fermented to obtain the new composition, and the composition belongs to a natural alpha-glucosidase inhibitor, and is low in price and environmentally friendly; the preparation technology is easy to industrialize. The inonotus obliquus submerged fermentation product is suitable for a broad crowd of people, convenient to eat and capable of serving as a daily drink to be drunk by people with diabetes, will not generate adverse reactions such as abdominal discomfort, flatulence and exsufflation, and can achieve a health-care effect after being drunk by average persons.
Description
Technical field
The present invention relates to technical field of biological fermentation is and in particular to a kind of birch fuscoporia ferrugineo-fusca teng submerged fermentation product and purposes.
Background technology
Diabetes and its complication have become as the serious the third-largest killer threatening human health.Currently for II type glycosuria
The oral antidiabetic drug of disease has：Sulfonylurea, biguanideses, thiazolidinedioneses and alpha-glucosidase inhibitor, wherein α-Fructus Vitis viniferae
Glycosidase inhibitor is because of the energy absorption to glucose for the delaying human body, and does not suppress protein and the absorption of fat, therefore clinically can
Effectively reduce post-prandial glycemia.The alpha-glucosidase inhibitor of listing mainly has：Acarbose, Voglibose and meter Ge Lie
Alcohol.Although these three alpha-glucosidase inhibitor of current listing have the effect of good reduction post-prandial glycemia, its valency
Lattice are higher, there is also the untoward reaction such as abdominal discomfort, flatulence, aerofluxuss, and mostly are synthetic drug.With people to healthy day
Benefit concern, obtains natural alpha-glucosidase inhibitor from Chinese medicine, develops safe and reliable, low toxicity and user further
Just new natural hypoglycemic medicine becomes a kind of trend.
Inonqqus obliquus are a kind of rare medicinal fungis, are praised as " glossy ganoderma in siberia ", tool in terms for the treatment of diabetes
There is the advantage of uniqueness.However, the growth only on the Betula lived of Inonqqus obliquus wild varieties just has good medicine in 10～15 years
With being worth, wild Inonqqus obliquus price is sufficiently expensive, and the rare of resource is difficult to meet the needs producing and studying.
Liquid submerged fermentation technology is to solve a kind of effective ways of Inonqqus obliquus wild resource shortage.In liquid fermentation mistake
Can produce in Cheng Zhong, mycelium and fermentation liquid and sclerotium or the same or analogous chemical composition of sporophore and pharmacologically active.One
Aspect mycelium is bred in a large number, and another aspect thalline is secreted metabolism in fermentation liquid and gone out polysaccharide, polypeptide, alkaloid, terpenoid
Multiple stimulation metabolite such as thing, sterol, enzyme, nucleic acid, aminoacid, vitamin, phytohormone, these metabolites have mostly
Strengthen the physiological functions such as immunity, anticancer, antiinflammatory, blood sugar lowering, antibacterial, antiviral, defying age, antiulcer.Sent out by deep liquid
Ferment, can quickly, the compositionss that stable, the acquisition of scale has given efficacy.
From tunning, the traditional method of effective component extracting has：(1) carried out with silica gel column chromatography etc. after extracting separating.
(2) tunning (mycelia and bacterium solution) is blended together as product to use.The problem of method (1) is introduce in experiment
Substantial amounts of organic solvent, and workload is big, poor repeatability.The removal that later stage remains toxic solvent is also very serious asking
Topic.The problem of method (2) is that the species of compound is complicated, if the simple use that mixes, can give rear quality standard
Formulation increase difficulty, in addition the pharmacological action pattern of different types of compound and effect might have very big difference, single
Pure mixing is lost specific aim.
Content of the invention
For solving defect in prior art, the present invention provides a kind of birch fuscoporia ferrugineo-fusca teng submerged fermentation product, is sent out by suitable
Fermenting process and tunning extracting method, obtain a kind of natural alpha-glucosidase inhibitor, that is, the present invention obtains
Tunning compositionss A, compositionss B and compositionss C, this tunning has preferable blood sugar decreasing effect.
For achieving the above object, the present invention adopts the following technical scheme that：
The present invention uses biofermentation technique, obtains birch fuscoporia ferrugineo-fusca teng submerged fermentation product, and described tunning includes combining
Thing A, compositionss B and compositionss C, described tunning is prepared by the following method and obtains：
(1) PDA solid medium, PDA liquid medium and culture medium of edible fungus are prepared respectively；Described culture medium of edible fungus
Formula is：Glucose 20.0g, peptone 4.0g, KH2PO42.0g、MgSO41.0g and water 1000ml, pH are natural；
(2) in the PDA solid medium after Inonqqus obliquus strain transfer extremely being sterilized and cools down through 121 DEG C, 20～35 DEG C
Under the conditions of culture 7～14 days a generation pass on strain；
(3) by PDA liquid medium through 121 DEG C of sterilizing 10～30min, cooling；Then a generation is passed on strain to be seeded to
Carry out level liquid fermentation culture in PDA liquid medium, cultivate 7～20 days at 20～35 DEG C, shaking speed is 50～320r/
Min, then proceed to PDA liquid medium by 10% inoculum concentration and carry out secondary liquid fermentation culture, cultivate 1 under the conditions of 20～35 DEG C
～14 days, shaking speed 50～320r/min, finally proceed to PDA liquid medium by 10% inoculum concentration and carry out tertiary liquid fermentation
Culture, cultivates 1～14 day at 20～35 DEG C, shaking speed 50～320r/min, obtains tunning；(wherein it is preferred to, one
Level liquid fermentation and culture is to cultivate 14 days at 28 DEG C, and shaking speed is 160r/min, secondary liquid fermentation culture and three-level liquid
Body fermentation culture is all to cultivate 7 days under the conditions of 28 DEG C, shaking speed 160r/min.)
(4) tunning is carried out sucking filtration, separate mycelia and bacterium solution, mycelia crushed after being dried；Macroporous resin column in bacterium solution
Afterwards, use water, 30% ethanol and 50% ethanol gradient elution respectively, respectively obtain water section, 30% ethanolic moiety and 50% ethanol
Three kinds of flow points of part；These three flow points are containing birch fuscoporia ferrugineo-fusca teng submerged fermentation product compositions A, compositionss B and combination respectively
Thing C.
Wherein, above-mentioned step (3) can also be replaced with following methods：Fermentation is amplified using 200L fermentation tank, specially：
By PDA liquid medium through 121 DEG C of sterilizing 10～30min, cooling, a generation is passed on strain and is seeded to level liquid culture medium
In, cultivate 7～20 days at 20～35 DEG C, shaking speed 50～320r/min, then proceed to secondary liquid by 10% inoculum concentration and send out
In ferment culture medium, cultivate 1～14 day at 20～35 DEG C, shaking speed 50～320r/min, finally proceed to by 10% inoculum concentration and send out
In fermentation tank culture medium, cultivate 1～14 day at 20～35 DEG C, rotating speed 50～320r/min；
Described level liquid culture medium and secondary liquid fermentation medium all using PDA liquid medium, described fermentation tank
Culture medium adopts culture medium of edible fungus.
Preferably, when 200L fermentation tank amplifies fermentation, in level liquid culture medium, cultivate 14 days at 28 DEG C, shaking table turns
Fast 180r/min；In secondary liquid fermentation medium, cultivate 7 days at 28 DEG C, shaking speed 180r/min, in fermentor cultivation
In base, cultivate 7 days at 28 DEG C, rotating speed 55r/min.
After obtaining above-mentioned three kinds of flow points, applicant has further proceeded with following mensure, and three kinds of flow points are concentrated respectively, cold
Lyophilizing is dry, weighs, and measures the content of total sugar content, DNS method survey reducing sugar with phend-sulphuric acid；The polysaccharide recording compositionss A contains
Amount is not less than 75%, and the polyoses content of compositionss B is not higher than 35%, and the polyoses content of compositionss C is not higher than 4%.
And also it is experimentally confirmed described birch fuscoporia ferrugineo-fusca teng submerged fermentation product compositions A, compositionss B and compositionss C
It is respectively provided with alpha-glucosaccharase enzyme inhibition activity, there is effect of lowering blood sugar.
Beneficial effects of the present invention are：
(1) present invention adopts suitable fermentation process that birch fuscoporia ferrugineo-fusca teng submerged fermentation product, this preparation method effective gram are obtained
The shortcoming having taken two kinds of traditional methods in prior art.Effective ingredient due to playing hypoglycemic activity in bacterium solution is mainly polysaccharide
Compound, next is only triterpenoid compound, and macroporous resin does not adsorb to polysaccharide compound, and triterpenoid compound is had again
There is good adsorption and desorption effect, so the present invention is according to the feature of type of compounds in fermentation liquid, innovation choice macropore
Resin carries out to bacterium solution separating, and not only can separate the polysaccharide in bacterium solution and triterpenoid compound well, farthest
Reduce the loss rate of sample, and be easy to the quality standard formulation in later stage and the pharmacological action pattern of further investigated compositionss,
Simple in production operation afterwards, reproducible, organic solvent pollution, environmental protection.
(2) present invention uses biofermentation technique, and the natural α obtaining from the liquid submerged fermentation product of Inonqqus obliquus-
Glucosidase inhibitor is a kind of new compositionss, and said composition is cheap, and preparation technology easily realizes industrialization, and green
Environmental protection.Suitable crowd extensively, and instant, can as the daily drinks Instant Drinks of diabetes patient, will not produce abdominal discomfort,
The untoward reaction such as flatulence, aerofluxuss.Normal person drinks the effect that can reach health care.
(3) present invention passes through the natural alpha-glucosidase inhibitor obtaining from Inonqqus obliquus liquid submerged fermentation product
New compositions, can solve the problems, such as presently commercially available alpha-glucosidase inhibitor, and blood sugar decreasing effect is obvious, preparation technology
Easily realize industrialization, cheap, environmental protection, and can quickly, stable, scale obtain, can be developed further into
Medicine.Said composition can also be used in the deep processing of beverage, Folium Camelliae sinensis etc. as food additive, make microcapsule and be applied to protect
Strong product field.Additionally, entering in cosmetic field, tobacco business, textile industry, feedstuff industry, frontier branch of science or even medicine field
Row application.
Brief description
Fig. 1 is birch fuscoporia ferrugineo-fusca teng submerged fermentation product preparation technology flow chart of the present invention.
Specific embodiment
It is more fully described the specific embodiment of the present invention below with reference to accompanying drawings.Although showing the present invention's in accompanying drawing
Specific embodiment is it being understood, however, that may be realized in various forms the present invention and should not being limited by embodiments set forth here
System.On the contrary, these embodiments are provided to be able to be best understood from the present invention, and can be complete by the scope of the present invention
Convey to those skilled in the art.
If mentioned "comprising" in the middle of description in the whole text and claim or " inclusion " they are an open language, therefore should
It is construed to " comprise but be not limited to ".Description subsequent descriptions are to implement the better embodiment of the present invention, and so described description is
For the purpose of the rule of description, it is not limited to the scope of the present invention.Protection scope of the present invention is when regarding appended power
Profit requires defined person to be defined.
Embodiment 1
Birch fuscoporia ferrugineo-fusca teng submerged fermentation product, including compositionss A, compositionss B and compositionss C, described tunning pass through with
Lower section method is prepared：
(1) PDA solid medium, PDA liquid medium and culture medium of edible fungus are prepared respectively；Described culture medium of edible fungus
Formula is：Glucose 20.0g, peptone 4.0g, KH2PO42.0g、MgSO41.0g and water 1000ml, pH are natural；
(2) in the PDA solid medium after Inonqqus obliquus strain transfer extremely being sterilized and cools down through 121 DEG C, 35 DEG C of conditions
Lower culture obtains a generation for 7 days and passes on strain；
(3) by PDA liquid medium through 121 DEG C of sterilizing 10min, cooling；Then a generation is passed on strain and be seeded to PDA liquid
Carry out level liquid fermentation culture in body culture medium, cultivate 7 days at 35 DEG C, shaking speed is 320r/min, then by 10% connect
The amount of kind proceeds to PDA liquid medium and carries out secondary liquid fermentation culture, cultivates 5 days, shaking speed 160r/min under the conditions of 35 DEG C,
Finally proceed to PDA liquid medium by 10% inoculum concentration and carry out tertiary liquid fermentation culture, cultivate 14 days at 35 DEG C, shaking speed
50r/min, obtains tunning；
(4) tunning is carried out sucking filtration, separate mycelia and bacterium solution, mycelia crushed after being dried；Macroporous resin column in bacterium solution
Afterwards, use water, 30% ethanol and 50% ethanol gradient elution respectively, respectively obtain water section, 30% ethanolic moiety and 50% ethanol
Three kinds of flow points of part；These three flow points are containing birch fuscoporia ferrugineo-fusca teng submerged fermentation product compositions A, compositionss B and combination respectively
Thing C.
Embodiment 2
Birch fuscoporia ferrugineo-fusca teng submerged fermentation product, including compositionss A, compositionss B and compositionss C, described tunning pass through with
Lower section method is prepared (its method flow is as shown in Figure 1)：
(1) PDA solid medium, PDA liquid medium and culture medium of edible fungus are prepared respectively；Described culture medium of edible fungus
Formula is：Glucose 20.0g, peptone 4.0g, KH2PO42.0g、MgSO41.0g and water 1000ml, pH are natural；
(2) in the PDA solid medium after Inonqqus obliquus strain transfer extremely being sterilized and cools down through 121 DEG C, 28 DEG C of conditions
Lower culture obtains a generation for 10 days and passes on strain；
(3) by PDA liquid medium through 121 DEG C of sterilizing 20min, cooling；Then a generation is passed on strain and be seeded to PDA liquid
Carry out level liquid fermentation culture (one-level shaking flask) in body culture medium, cultivate 14 days at 28 DEG C, shaking speed is 160r/min, then
Proceed to PDA liquid medium by 10% inoculum concentration and carry out secondary liquid fermentation culture (second-level shake flask), under the conditions of 28 DEG C, cultivate 7
My god, shaking speed 160r/min, finally proceed to PDA liquid medium by 10% inoculum concentration and carry out tertiary liquid fermentation culture (three
Level shaking flask), cultivate 7 days at 28 DEG C, shaking speed 160r/min, obtain tunning；
(4) tunning is carried out sucking filtration, separate mycelia and bacterium solution, mycelia crushed after being dried；Macroporous resin column in bacterium solution
Afterwards, use water, 30% ethanol and 50% ethanol gradient elution respectively, respectively obtain water section, 30% ethanolic moiety and 50% ethanol
Three kinds of flow points of part；These three flow points are containing birch fuscoporia ferrugineo-fusca teng submerged fermentation product compositions A, compositionss B and combination respectively
Thing C.
Embodiment 3
Birch fuscoporia ferrugineo-fusca teng submerged fermentation product, including compositionss A, compositionss B and compositionss C, described tunning pass through with
Lower section method is prepared：
(1) PDA solid medium, PDA liquid medium and culture medium of edible fungus are prepared respectively；Described culture medium of edible fungus
Formula is：Glucose 20.0g, peptone 4.0g, KH2PO42.0g、MgSO41.0g and water 1000ml, pH are natural；
(2) in the PDA solid medium after Inonqqus obliquus strain transfer extremely being sterilized and cools down through 121 DEG C, 20 DEG C of conditions
Lower culture obtains a generation for 14 days and passes on strain；
(3) by PDA liquid medium through 121 DEG C of sterilizing 30min, cooling；Then a generation is passed on strain and be seeded to PDA liquid
Carry out level liquid fermentation culture in body culture medium, cultivate 20 days at 20 DEG C, shaking speed is 280r/min, then by 10% connect
The amount of kind proceeds to PDA liquid medium and carries out secondary liquid fermentation culture, cultivates 4 days, shaking speed 320r/min under the conditions of 35 DEG C,
Finally proceed to PDA liquid medium by 10% inoculum concentration and carry out tertiary liquid fermentation culture, cultivate 14 days at 20 DEG C, shaking speed
50r/min, obtains tunning；
(4) tunning is carried out sucking filtration, separate mycelia and bacterium solution, mycelia crushed after being dried；Macroporous resin column in bacterium solution
Afterwards, use water, 30% ethanol and 50% ethanol gradient elution respectively, respectively obtain water section, 30% ethanolic moiety and 50% ethanol
Three kinds of flow points of part；These three flow points are containing birch fuscoporia ferrugineo-fusca teng submerged fermentation product compositions A, compositionss B and combination respectively
Thing C.
Embodiment 4
Birch fuscoporia ferrugineo-fusca teng submerged fermentation product, including compositionss A, compositionss B and compositionss C, described tunning pass through with
Lower section method is prepared：
(1) PDA solid medium, PDA liquid medium and culture medium of edible fungus are prepared respectively；Described culture medium of edible fungus
Formula is：Glucose 20.0g, peptone 4.0g, KH2PO42.0g、MgSO41.0g and water 1000ml, pH are natural；
(2) in the PDA solid medium after Inonqqus obliquus strain transfer extremely being sterilized and cools down through 121 DEG C, 20 DEG C of conditions
Lower culture obtains a generation for 12 days and passes on strain；
(3) 200L fermentation tank is adopted to amplify fermentation：By PDA liquid medium through 121 DEG C of sterilizing 20min, cooling, by a generation
Pass on strain to be seeded in level liquid culture medium, cultivate 20 days at 20 DEG C, shaking speed 50r/min, then the inoculation pressing 10%
Amount proceeds in secondary liquid fermentation medium, cultivates 7 days, shaking speed 180r/min, finally turn by 10% inoculum concentration at 35 DEG C
Enter in fermentation tank culture medium, cultivate 14 days at 20 DEG C, rotating speed 320r/min；
Described level liquid culture medium and secondary liquid fermentation medium all using PDA liquid medium, described fermentation tank
Culture medium adopts culture medium of edible fungus；
(4) tunning is carried out sucking filtration, separate mycelia and bacterium solution, mycelia crushed after being dried；Macroporous resin column in bacterium solution
Afterwards, use water, 30% ethanol and 50% ethanol gradient elution respectively, respectively obtain water section, 30% ethanolic moiety and 50% ethanol
Three kinds of flow points of part；These three flow points are containing birch fuscoporia ferrugineo-fusca teng submerged fermentation product compositions A, compositionss B and combination respectively
Thing C.
Embodiment 5
Birch fuscoporia ferrugineo-fusca teng submerged fermentation product, including compositionss A, compositionss B and compositionss C, described tunning pass through with
Lower section method is prepared：
(1) PDA solid medium, PDA liquid medium and culture medium of edible fungus are prepared respectively；Described culture medium of edible fungus
Formula is：Glucose 20.0g, peptone 4.0g, KH2PO42.0g、MgSO41.0g and water 1000ml, pH are natural；
(2) in the PDA solid medium after Inonqqus obliquus strain transfer extremely being sterilized and cools down through 121 DEG C, 28 DEG C of conditions
Lower culture obtains a generation for 8 days and passes on strain；
(3) 200L fermentation tank is adopted to amplify fermentation, specially：By PDA liquid medium through 121 DEG C of sterilizing 20min, cold
But, a generation is passed on strain to be seeded in level liquid culture medium, cultivate 14 days at 28 DEG C, shaking speed 180r/min, then press
10% inoculum concentration proceeds in secondary liquid fermentation medium, cultivates 7 days, shaking speed 180r/min, finally press at 28 DEG C
10% inoculum concentration proceeds in fermentation tank culture medium, cultivates 7 days, rotating speed 180r/min at 28 DEG C；
Described level liquid culture medium and secondary liquid fermentation medium all using PDA liquid medium, described fermentation tank
Culture medium adopts culture medium of edible fungus；
(4) tunning is carried out sucking filtration, separate mycelia and bacterium solution, mycelia crushed after being dried；Macroporous resin column in bacterium solution
Afterwards, use water, 30% ethanol and 50% ethanol gradient elution respectively, respectively obtain water section, 30% ethanolic moiety and 50% ethanol
Three kinds of flow points of part；These three flow points are containing birch fuscoporia ferrugineo-fusca teng submerged fermentation product compositions A, compositionss B and combination respectively
Thing C.
Embodiment 6
Birch fuscoporia ferrugineo-fusca teng submerged fermentation product, including compositionss A, compositionss B and compositionss C, described tunning pass through with
Lower section method is prepared：
(1) PDA solid medium, PDA liquid medium and culture medium of edible fungus are prepared respectively；Described culture medium of edible fungus
Formula is：Glucose 20.0g, peptone 4.0g, KH2PO42.0g、MgSO41.0g and water 1000ml, pH are natural；
(2) in the PDA solid medium after Inonqqus obliquus strain transfer extremely being sterilized and cools down through 121 DEG C, 30 DEG C of conditions
Lower culture obtains a generation for 8 days and passes on strain；
(3) 200L fermentation tank is adopted to amplify fermentation, specially：By PDA liquid medium through 121 DEG C of sterilizing 30min, cold
But, a generation is passed on strain to be seeded in level liquid culture medium, cultivate 12 days at 30 DEG C, shaking speed 200r/min, then press
10% inoculum concentration proceeds in secondary liquid fermentation medium, cultivates 10 days, shaking speed 200r/min, finally press at 25 DEG C
10% inoculum concentration proceeds in fermentation tank culture medium, cultivates 10 days, rotating speed 200r/min at 25 DEG C；
Described level liquid culture medium and secondary liquid fermentation medium all using PDA liquid medium, described fermentation tank
Culture medium adopts culture medium of edible fungus.
(4) tunning is carried out sucking filtration, separate mycelia and bacterium solution, mycelia crushed after being dried；Macroporous resin column in bacterium solution
Afterwards, use water, 30% ethanol and 50% ethanol gradient elution respectively, respectively obtain water section, 30% ethanolic moiety and 50% ethanol
Three kinds of flow points of part；These three flow points are containing birch fuscoporia ferrugineo-fusca teng submerged fermentation product compositions A, compositionss B and combination respectively
Thing C.
In order to verify the effect of tunning further, applicant has also carried out following test：
Respectively three kinds obtained using said method flow points are concentrated, lyophilization, weigh.Measured with phend-sulphuric acid
Total sugar content, DNS method surveys the content of reducing sugar.The polyoses content of birch fuscoporia ferrugineo-fusca teng submerged fermentation compositionss A obtaining through this technique
It is not less than 75%.The polyoses content of birch fuscoporia ferrugineo-fusca teng submerged fermentation compositionss B is not higher than 35%, birch fuscoporia ferrugineo-fusca teng submerged fermentation compositionss
The polyoses content of C is not higher than 4%.
And lived with the alpha-glucosaccharase enzyme level of determination of glucose oxidase compositionss A, compositionss B and compositionss C
Property.Test result shows that birch fuscoporia ferrugineo-fusca teng submerged fermentation compositionss A, compositionss B and compositionss C all have good alpha-glucosidase
Inhibitory activity.Draw especially by following controlled trial.
(1), with Acarbose as positive control, when concentration is 100mg/ml, the suppression ratio of Acarbose is 99%, Pyropolyporus fomentarius (L.ex Fr.) Teng
The suppression ratio of pore fungi submerged fermentation compositionss A, compositionss B and compositionss C is respectively 99%, 92%, 98%, and effect is with A Kabo
Sugar is quite.
(2) continue with Acarbose as positive control, when concentration is reduced to 25mg/ml further, the suppression of Acarbose
Rate processed is 99%, the suppression ratio of birch fuscoporia ferrugineo-fusca teng submerged fermentation compositionss A, compositionss B and compositionss C is respectively 98%, 26%,
29%, the blood sugar decreasing effect of birch fuscoporia ferrugineo-fusca teng submerged fermentation compositionss A is optimum, suitable with Acarbose effect.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all substantive in the present invention
Any modification, equivalent and simple modifications of being made in content etc., should be included within the scope of the present invention.
Claims (6)
1. birch fuscoporia ferrugineo-fusca teng submerged fermentation product is it is characterised in that described tunning includes compositionss A, compositionss B and compositionss
C, described tunning is prepared by the following method and obtains：
(1) PDA solid medium, PDA liquid medium and culture medium of edible fungus are prepared respectively；Described culture medium of edible fungus formula
For：Glucose 20.0g, peptone 4.0g, KH2PO42.0g、MgSO41.0g and water 1000ml, pH are natural；
(2) in the PDA solid medium after Inonqqus obliquus strain transfer extremely being sterilized and cools down through 121 DEG C, 20～35 DEG C of conditions
Lower culture obtains a generation for 7～14 days and passes on strain；
(3) by PDA liquid medium through 121 DEG C of sterilizing 10～30min, cooling；Then a generation is passed on strain and be seeded to PDA liquid
Carry out level liquid fermentation culture in body culture medium, cultivate 7～20 days at 20～35 DEG C, shaking speed is 50～320r/min,
Proceed to PDA liquid medium by 10% inoculum concentration again and carry out secondary liquid fermentation culture, under the conditions of 20～35 DEG C, cultivate 1～14
My god, shaking speed 50～320r/min, finally proceed to PDA liquid medium by 10% inoculum concentration and carry out tertiary liquid fermentation training
Support, cultivate 1～14 day at 20～35 DEG C, shaking speed 50～320r/min, obtain tunning；
(4) tunning is carried out sucking filtration, separate mycelia and bacterium solution, mycelia crushed after being dried；After macroporous resin column in bacterium solution, point
Not Yong water, 30% ethanol and 50% ethanol gradient elution, respectively obtain water section, 30% ethanolic moiety and 50% ethanolic moiety three
Plant flow point；These three flow points are containing birch fuscoporia ferrugineo-fusca teng submerged fermentation product compositions A, compositionss B and compositionss C respectively.
2. birch fuscoporia ferrugineo-fusca teng submerged fermentation product according to claim 1 is it is characterised in that step (3) can also be with following
Method replaces：Fermentation is amplified using 200L fermentation tank, specially：By PDA liquid medium through 121 DEG C of sterilizing 10～30min, cold
But, a generation is passed on strain to be seeded in level liquid culture medium, cultivate 7～20 days at 20～35 DEG C, shaking speed 50～
320r/min, then proceed in secondary liquid fermentation medium by 10% inoculum concentration, cultivate 1～14 day at 20～35 DEG C, shaking table
Rotating speed 50～320r/min, finally proceeds in fermentation tank culture medium by 10% inoculum concentration, cultivates 1～14 day at 20～35 DEG C, turns
Fast 50～320r/min；
Described level liquid culture medium and secondary liquid fermentation medium all using PDA liquid medium, described fermentor cultivation
Base adopts culture medium of edible fungus.
3. birch fuscoporia ferrugineo-fusca teng submerged fermentation product according to claim 1 it is characterised in that level liquid fermentation culture be
Cultivate 14 days at 28 DEG C, shaking speed is 160r/min, secondary liquid fermentation culture and tertiary liquid fermentation culture are all 28
Cultivate 7 days under the conditions of DEG C, shaking speed 160r/min.
4. birch fuscoporia ferrugineo-fusca teng submerged fermentation product according to claim 2 is it is characterised in that in level liquid culture medium,
Cultivate 14 days at 28 DEG C, shaking speed 180r/min；In secondary liquid fermentation medium, cultivate 7 days at 28 DEG C, shaking speed
180r/min, in fermentation tank culture medium, cultivates 7 days at 28 DEG C, rotating speed 55r/min.
5. birch fuscoporia ferrugineo-fusca teng submerged fermentation product according to claim 1 is it is characterised in that concentrate three kinds of flow points respectively,
Lyophilization, weighs, and measures the content of total sugar content, DNS method survey reducing sugar with phend-sulphuric acid；Record the polysaccharide of compositionss A
Content is not less than 75%, and the polyoses content of compositionss B is not higher than 35%, and the polyoses content of compositionss C is not higher than 4%.
6. the purposes of the birch fuscoporia ferrugineo-fusca teng submerged fermentation product described in any one of Claims 1 to 5 is it is characterised in that described Pyropolyporus fomentarius (L.ex Fr.) Teng
Pore fungi submerged fermentation compositionss A, compositionss B and compositionss C are respectively provided with alpha-glucosaccharase enzyme inhibition activity, have fall blood
Sugared effect.
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| CN107173394A (en) * | 2017-07-18 | 2017-09-19 | 黑龙江省科学院微生物研究所 | Crop growth regulator, preparation method and its usage based on Inonotus obliquus |
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| CN112703967A (en) * | 2020-12-07 | 2021-04-27 | 贵州大学 | Method for preparing wood rot fungus liquid strain by using yellow serofluid and bean dregs |
| CN114587007A (en) * | 2022-02-23 | 2022-06-07 | 张淑军 | Health-preserving herbal cigarette with lotus leaf and gynostemma pentaphylla as main raw materials |
| CN114587007B (en) * | 2022-02-23 | 2023-08-04 | 张淑军 | A health herbal cigarette prepared from folium Nelumbinis and herba Gynostemmatis |
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