CN106434337A - Ultrasonic cell disruption device based on spherical wave resonance principle - Google Patents
Ultrasonic cell disruption device based on spherical wave resonance principle Download PDFInfo
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- CN106434337A CN106434337A CN201610782529.2A CN201610782529A CN106434337A CN 106434337 A CN106434337 A CN 106434337A CN 201610782529 A CN201610782529 A CN 201610782529A CN 106434337 A CN106434337 A CN 106434337A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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Abstract
The invention discloses an ultrasonic cell disruption device based on a spherical wave resonance principle. The ultrasonic cell disruption device comprises an ultrasonic wave generator, a transducer and amplitude-change poles, wherein a medium container is arranged at one side of the ultrasonic wave generator; the transducer is arranged between the ultrasonic wave generator and the medium container; the amplitude-change poles are connected to the transducer and are arranged in the medium container; a sample container is arranged in the medium container; and the sample container is fixedly arranged in the medium container by virtue of a sample container fixing device. The ultrasonic cell disruption device provided by the invention has the advantages that the ultrasonic cell disruption device has the property that spherical waves are formed and enriched in the sample container on the basis of ultrasonic waves under the precondition of not increasing the energy of the ultrasonic energy generator, so that an ultrasonic tissue disruption effect is enhanced and a material disruption effect is effectively improved; a normal disruption operation cannot be affected; and the sample container can be taken out from the medium container, so as to facilitate cleaning of the medium container and to avoid the phenomenon that medium liquid remains in the medium container.
Description
Technical field
The invention belongs to ultrasonic disruption equipment technical field is and in particular to a kind of ultrasonic based on spherical wave resonance principle
Ripple cell breaking plant.
Background technology
Ultrasonic disruption device is cavitation effect in a liquid using ultrasound wave, makes liquid produce numerous minute bubbles,
By the explosion of minute bubbles, so that the solid particle in liquid or cell tissue crush, it can be used for multiple animals and plants, disease
Poison, cell, antibacterial and tissue broken, also can be used to simultaneously emulsifying, discrete, homogenize, extract, froth breaking, clear, nano material
Preparation, dispersion and acceleration chemical reaction etc., ultrasonic disruption device is widely used in biology, microbiology, physicss, animal
The fields such as, agronomy, pharmacy, chemical industry, sewage disposal, nano material.
Traditional ultrasonic disruption device is made up of supersonic generator, transducer and three parts of sound proof box, transducer
Operationally because vibration can produce larger noise, in actual use, typically all transducer is put in sound proof box, can
It is effectively isolated noise, have a special broken bottle in sound proof box, the horn on transducer is stretched into the liquid in broken bottle
In, thus cell or tissue is carried out with fragmentation procedure, the structure setting of traditional ultrasonic disruption device is unreasonable, is unfavorable for locating
The radiating of reason liquid, treatment fluid can remain at the dead angle of broken bottle, is not easy to the cleaning of broken bottle.
Ultrasonic cell-break device has been widely used in the middle of scientific experimentss and actual production, by ultrasound wave in liquid
Cavitation effect in body, causes the covibration in material structure, to destroy the structure of material using resonance, and that commonly uses at present is super
, mainly based on scattered wave and T-shaped ripple, scattered wave scope is wide, and intensity is relatively weak for sonication device, although T-shaped ripple is strong
Degree is higher, but rapidly to surrounding diffusion after its generation, can't form the accumulation of Wave energy, crushing effect depends primarily on ripple
First set reaction after dynamic impact, because Wave energy can not be assembled, leads to material crushing effect not good, the experiment in impact later stage
And production and processing, inefficiency.
Content of the invention
It is an object of the invention to provide a kind of ultrasonic cell-break device based on spherical wave resonance principle, existing to solve
There is Wave energy in technology can not assemble, material crushing effect is not good, affect experiment and the production and processing in later stage, work efficiency
Low, media fluid radiating is bad, the problem that media fluid residual is not easy to clear up.
For reaching above-mentioned purpose, the technical scheme is that：A kind of supersonic cell based on spherical wave resonance principle
Breaker, including the supersonic generator 2 being connected by transmission line 1, transducer 3 and horn 4, supersonic generator 2
Side is provided with media Containers 5, and transducer 3 is arranged between supersonic generator 2 and media Containers 5, horn 4 and transducer 3
Connect and be arranged in media Containers 5, in media Containers 5, be provided with sample container 6, sample container 6 is by the fixing dress of sample container
Put and be fixed in media Containers 5.
Preferably, sample container 6 is spherical container, and sample container 6 is provided with tremie pipe 7, one end of tremie pipe 7 and sample
Container 6 is fixedly connected, and the other end is extended on outside media Containers 5.
Preferably, sample container fixing device includes fixed frame 8, connecting rod 9 and spacing ring 10, fixed frame 8 and Jie
The inwall of matter container 5 is adapted, and the internal diameter of spacing ring 9 is adapted with the external diameter of sample container 6, and fixed frame 8 passes through connecting rod 9
It is fixedly connected with spacing ring 10.
Preferably, it is provided with the jacking block 11 being adapted with sample container 6, the side of jacking block 11 and sample container 6 in connecting rod 9
Contact, opposite side is connected with spring 12.
Preferably, the connecting portion of spacing ring 10 and connecting rod 9 is provided with the bottom gag lever post 13 being adapted with sample container 6.
Preferably, the inwall of media Containers 5 is provided with limited block 14.
Preferably, it is filled with external agency 15 in media Containers 5, in sample container 6, be filled with interior media 16, outside Jie
The tall and big spherical portion in sample container 6 of liquid of matter 15, the tall and big spherical portion in sample container 6 of liquid of interior media 16 and less than outer
The liquid of portion's medium 15 is high.
Preferably, sample container 6 to horn 4 distance be external agency 15 in ultrasonic frequency wavelength integral multiple,
The internal diameter of sample container 6 is the integral multiple of ultrasonic frequency wavelength in interior media 16, and the wall thickness of sample container 6 is less than internal Jie
The 1/10 of ultrasonic frequency wavelength in matter 16 material therefor, the density of external agency 15 is more than the density of interior media 16.
Preferably, the top of media Containers 5 is provided with top cover 17, top cover 17 by hinge 18 with media Containers 5 activity even
Connect.
Preferably, top cover 17 is provided with the through hole 19 that tremie pipe 7 can pass through, and through hole 19 is socketed with tremie pipe sealing ring
20, the side plate of media Containers 5 is provided with the top seal pad 21 corresponding with top cover 17.
It is an advantage of the current invention that：The present invention, on the premise of not improving supersonic generator energy, is existed using ultrasound wave
Form spherical wave in sample container and the feature that is enriched with is it is achieved that strengthening to ultrasonic wave tissue crushing effect, effectively
Improve the effect that material crushes, ultrasonic energy will not spread to surrounding, and sample container is by sample container fixing device
It is firmly fixed in media Containers, it is to avoid phenomenon that sample container is subjected to displacement is it is ensured that the stablizing of material shattering process
Property, effectively raise the work efficiency of breaker, the experiment for the later stage and production and processing provide facility, inside and outside two media
Good heat dissipation effect, does not interfere with and normally crushes work, sample container can be taken out in media Containers, facilitates media Containers
Cleaning, it is to avoid media fluid remains in the phenomenon in media Containers.
Brief description
Fig. 1 is the cross section structure diagram of the present invention.
Fig. 2 is the structural representation of sample container fixing device of the present invention.
Fig. 3 is the section view perspective view of the present invention.
Reference implication is as follows：Transmission line（1）, supersonic generator（2）, transducer（3）, horn（4）, medium appearance
Device（5）, sample container（6）, tremie pipe（7）, fixed frame（8）, connecting rod（9）, spacing ring（10）, jacking block（11）, spring
（12）, bottom gag lever post（13）, limited block（14）, external agency（15）, interior media（16）, top cover（17）, hinge（18）, lead to
Hole（19）, tremie pipe sealing ring（20）, top seal pad（21）.
Specific embodiment
The invention will be further described with specific embodiment for explanation below in conjunction with the accompanying drawings.
A kind of ultrasonic cell-break device based on spherical wave resonance principle as shown in Figure 1, Figure 2 and Figure 3, including logical
Cross supersonic generator 2, transducer 3 and the horn 4 of transmission line 1 connection, the side of supersonic generator 2 is provided with media Containers
5, transducer 3 is arranged between supersonic generator 2 and media Containers 5, and horn 4 is connected with transducer 3 and is arranged on medium
In container 5, in media Containers 5, it is provided with sample container 6, sample container 6 is fixed on media Containers 5 by sample container fixing device
Interior.
In order that forming sphere resonance in sample container 6, make the ultrasonic activation can be in the interior media 16 of sample container 6
Interior formation is enriched with, and improves crushing efficiency and crushing effect, and setting sample container 6 is spherical container, and sample container 6 is provided with blanking
Pipe 7, one end of tremie pipe 7 is fixedly connected with sample container 6, and the other end is extended on outside media Containers 5.
In order to ensure stability in external agency 15 for the sample container 6, it is to avoid sample container 6 body is in the work of ultrasound wave
Vibrated with lower, make the ultrasound wave in sample container 6 can form enrichment, make fixing more firm, the sample of sample container 6
Receptacle fixture includes fixed frame 8, connecting rod 9 and spacing ring 10, and fixed frame 8 is adapted with the inwall of media Containers 5,
The internal diameter of spacing ring 9 is adapted with the external diameter of sample container 6, and fixed frame 8 is fixedly connected with spacing ring 10 by connecting rod 9.
In order to be further ensured that the stability of sample container 6, it is provided with the top being adapted with sample container 6 in connecting rod 9
Block 11, the side of jacking block 11 is contacted with sample container 6, and opposite side is connected with spring 12.
In order to ensure the stability of sample container 6 spherical bottom so as to fixing is more firm, in spacing ring 10 and connection
The connecting portion of bar 9 is provided with the bottom gag lever post 13 being adapted with sample container 6.
In order to ensure the stability of sample container fixing device, it is facilitated to be fixed on the inwall of media Containers 5, in medium
The inwall of container 5 is provided with limited block 14.
In order to make the ultrasound wave in sample container 6 form enrichment further, improve crushing effect, fill out in media Containers 5
It is filled with external agency 15, in sample container 6, be filled with interior media 16, the tall and big ball in sample container 6 of liquid of external agency 15
Shape portion, the tall and big spherical portion in sample container 6 of liquid of interior media 16 and the liquid height less than external agency 15.
In order to improve broken efficiency and effect further, make ultrasound wave form enrichment in sample container 6, improve ultrasonic
The transmission speed of ripple, the distance of setting sample container 6 to horn 4 is the integer of ultrasonic frequency wavelength in external agency 15
Times, the internal diameter of sample container 6 is the integral multiple of ultrasonic frequency wavelength in interior media 16, and the wall thickness of sample container 6 is less than interior
The 1/10 of ultrasonic frequency wavelength in portion's medium 16 material therefor, the density of external agency 15 is more than the density of interior media 16.
Take out sample container 6 after crushing end-of-job for convenience, be provided with top cover 17, top cover at the top of media Containers 5
17 be flexibly connected with media Containers 5 by hinge 18.
In order to ensure the seal of media Containers, prevent external agency 15 from overflowing, make the more firm of tremie pipe 7 fixation,
Top cover 17 is provided with the through hole 18 that tremie pipe 7 can pass through, and through hole 18 is socketed with tremie pipe sealing ring 19, media Containers 5
Side plate is provided with the top seal pad 20 corresponding with top cover 17.
Embodiment one
The power of supersonic generator 2 is 100W, and the frequency of ultrasound wave is 20kHZ, and in media Containers 5, external agency 15 is water,
The conduction velocity of the ultrasound wave in water is 1473m/s, and wavelength is 7.365cm, and the distance of sample container 6 to horn 4 is outside
The integral multiple of ultrasonic frequency wavelength in medium 15, thus the distance arranging sample container 6 to horn 4 is 73.65cm；Internal
Medium 16 is ethanol, and the conduction velocity of the sound wave in ethanol is 1168m/s, and wavelength is 5.84cm, and the internal diameter of sample container 6 is interior
The integral multiple of ultrasonic frequency wavelength in portion's medium 16, thus the internal diameter arranging sample container 6 is 58.4cm；Sample container 6
Material is glass, and the conduction velocity of the ultrasound wave in glass is 5640m/s, and wavelength is 28.2cm, and the wall thickness of sample container 6 is less than
The 1/10 of ultrasonic frequency wavelength in interior media 16 material therefor, thus the wall thickness arranging sample container 6 is less than 2.82cm.
So that DPPH is in the dissolving in the middle of ethanol as a example, DPPH is the important examination of analysis antioxidant radical scavenging activity
Agent, water insoluble, it is dissolvable in water ethanol, but its rate of dissolution is extremely slow, 0.1gDPPH is dissolved completely in the middle of 250ml ethanol about
Time-consuming 6 hours；T-shaped ultrasonic unit is under Same Efficieney, the output condition of frequency（100W、20kHZ）, needing 30 minutes can be complete
Dissolving DPPH；Using the ultrasonic Treatment condition in the embodiment of the present invention one, can effectively accelerate dissolving in the middle of ethanol for the DPPH
Speed, can be completely dissolved for 5 minutes, effectively the grain breakage of DPPH and dissolved efficiency can be improved 6 times.
Embodiment two
The power of supersonic generator 2 is 200W, and ultrasonic frequency is 25kHZ, and the external agency 15 in media Containers 5 is 20%
Calcium chloride solution, the conduction velocity of the ultrasound wave in calcium chloride solution is 1531m/s, and wavelength is 6.124cm, sample container 6 to
The distance of horn 4 is the integral multiple of ultrasonic frequency wavelength in external agency 15, thus arranging sample container 6 to horn 4
Distance be 61.24cm；Interior media 16 is water, and the conduction velocity of the ultrasound wave in water is 1473m/s, and wavelength is 5.892cm,
The internal diameter of sample container 6 is the integral multiple of ultrasonic frequency wavelength in interior media 16, thus the internal diameter arranging sample container 6 is
58.92cm；The material of sample container 6 is lucite, and the conduction velocity of the ultrasound wave in lucite is 2692m/s, wavelength
For 10.77cm, the wall thickness of sample container 6 is less than 1/10 of ultrasonic frequency wavelength in interior media 16 material therefor, thus setting
The wall thickness putting sample container 6 is less than 1.07cm.
, T-shaped ultrasonic unit is in Same Efficieney, frequency taking by the heparin sodium in ultrasound-assisted extraction pulmonis Bovis seu Bubali as a example
Under output condition（200W、25kHZ）, heparin sodium extraction ratio can be improved 16%；At the ultrasound wave in the embodiment of the present invention two
Manage bar part, the extraction ratio of heparin sodium can reach 22%, heparin sodium auxiliary extraction efficiency can be improved 6%.
Embodiment three
The power of supersonic generator 2 is 400W, and ultrasonic frequency is 40kHZ, and the external agency 15 in media Containers 5 is 20%
Calcium chloride solution, the conduction velocity of the ultrasound wave in calcium chloride solution is 1531m/s, and wavelength is 3.8275cm, sample container 6 to
The distance of horn 4 is the integral multiple of ultrasonic frequency wavelength in external agency 15, thus arranging sample container 6 to horn 4
Distance be 76.55cm；Interior media 16 is milk, and the conduction velocity of the ultrasound wave in milk is 1473m/s, and wavelength is
3.6825cm, the internal diameter of sample container 6 is the integral multiple of ultrasonic frequency wavelength in interior media 16, thus arranging sample container
6 internal diameter is 73.65cm；The material of sample container 6 is lucite, and the conduction velocity of the ultrasound wave in lucite is
2692m/s, wavelength is 6.73cm, and the wall thickness of sample container 6 is less than ultrasonic frequency wavelength in interior media 16 material therefor
1/10, thus the wall thickness arranging sample container 6 is less than 0.673cm.
So that ultrasonic disruption is carried out to milk as a example, using the ultrasonic Treatment condition in the embodiment of the present invention three, equal
Under conditions of matter 5 minutes, the mean diameter of milk emulsion can be reduced to less than 1.0 microns.
Above-described be only the present invention preferred embodiments, technical scheme be not limited thereto it should be pointed out that
To those skilled in the art, under technology provided by the present invention enlightenment, as the common knowledge of this area, also
Other equivalent modifications and improvement can be made, also should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of ultrasonic cell-break device based on spherical wave resonance principle, including by transmission line（1）Connect is ultrasonic
Wave producer（2）, transducer（3）And horn（4）It is characterised in that：Described supersonic generator（2）Side be provided with medium
Container（5）, described transducer（3）It is arranged on supersonic generator（2）And media Containers（5）Between, described horn（4）With change
Can device（3）Connect and be arranged on media Containers（5）Interior, described media Containers（5）Inside it is provided with sample container（6）, described sample appearance
Device（6）Media Containers are fixed on by sample container fixing device（5）Interior.
2. as claimed in claim 1 a kind of ultrasonic cell-break device based on spherical wave resonance principle it is characterised in that：
Described sample container（6）For spherical container, described sample container（6）It is provided with tremie pipe（7）, described tremie pipe（7）One end
With sample container（6）It is fixedly connected, the other end is extended on media Containers（5）Outward.
3. as claimed in claim 2 a kind of ultrasonic cell-break device based on spherical wave resonance principle it is characterised in that：
Described sample container fixing device includes fixed frame（8）, connecting rod（9）And spacing ring（10）, described fixed frame（8）With Jie
Matter container（5）Inwall be adapted, described spacing ring（9）Internal diameter and sample container（6）External diameter be adapted, described fixed frame
Body（8）By connecting rod（9）With spacing ring（10）It is fixedly connected.
4. as claimed in claim 3 a kind of ultrasonic cell-break device based on spherical wave resonance principle it is characterised in that：
Described connecting rod（9）Inside it is provided with and sample container（6）The jacking block being adapted（11）, described jacking block（11）Side and sample container
（6）Contact, opposite side is connected with spring（12）.
5. as claimed in claim 4 a kind of ultrasonic cell-break device based on spherical wave resonance principle it is characterised in that：
Described spacing ring（10）And connecting rod（9）Connecting portion be provided with and sample container（6）The bottom gag lever post being adapted（13）.
6. as claimed in claim 5 a kind of ultrasonic cell-break device it is characterised in that：Described media Containers（5）Interior
Wall is provided with limited block（14）.
7. as claimed in claim 6 a kind of ultrasonic cell-break device based on spherical wave resonance principle it is characterised in that：
Described media Containers（5）Inside it is filled with external agency（15）, described sample container（6）Inside it is filled with interior media（16）, described
External agency（15）Liquid tall and big in sample container（6）Spherical portion, described interior media（16）Liquid tall and big in sample container
（6）Spherical portion and be less than external agency（15）Liquid high.
8. as claimed in claim 7 a kind of ultrasonic cell-break device based on spherical wave resonance principle it is characterised in that：
Described sample container（6）To horn（4）Distance be external agency（15）The integral multiple of middle ultrasonic frequency wavelength, described sample
This container（6）Internal diameter be interior media（16）The integral multiple of middle ultrasonic frequency wavelength, described sample container（6）Wall thickness little
In interior media（16）The 1/10 of ultrasonic frequency wavelength in material therefor, described external agency（15）Density be more than internal Jie
Matter（16）Density.
9. as claimed in claim 8 a kind of ultrasonic cell-break device based on spherical wave resonance principle it is characterised in that：
Described media Containers（5）Top be provided with top cover（17）, described top cover（17）By hinge（18）With media Containers（5）Activity
Connect.
10. a kind of ultrasonic cell-break device based on spherical wave resonance principle as claimed in claim 9, its feature exists
In：Described top cover（17）It is provided with tremie pipe（7）The through hole that can pass through（19）, described through hole（19）On to be socketed with tremie pipe close
Seal（20）, described media Containers（5）Side plate be provided with and top cover（17）Corresponding top seal pad（21）.
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| CN201610782529.2A CN106434337B (en) | 2016-08-31 | 2016-08-31 | A kind of ultrasonic cell-break device based on spherical wave resonance principle |
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| CN201610782529.2A CN106434337B (en) | 2016-08-31 | 2016-08-31 | A kind of ultrasonic cell-break device based on spherical wave resonance principle |
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| CN106434337B CN106434337B (en) | 2019-03-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112774533A (en) * | 2020-12-18 | 2021-05-11 | 昆山微电子技术研究院 | Solid-liquid dispersion device |
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| US5585044A (en) * | 1993-12-28 | 1996-12-17 | Hamamatsu Photonics K.K. | Liquid treating method and liquid treating apparatus |
| CN2581058Y (en) * | 2002-08-29 | 2003-10-22 | 程树群 | Emulsifying device for iodipin and medicinal preparation |
| CN101435064A (en) * | 2008-12-08 | 2009-05-20 | 清华大学 | High sound intensity ultrasonic processing apparatus for metal and alloy solidification and processing method thereof |
| US20120234625A1 (en) * | 2011-03-17 | 2012-09-20 | Covaris, Inc. | Acoustic treatment vessel and method for acoustic treatment |
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| CN1102362A (en) * | 1993-10-07 | 1995-05-10 | 索菲马有限公司 | A method and a device for sonicating and transmitting vibrations to a sonication liquid containing particles |
| US5585044A (en) * | 1993-12-28 | 1996-12-17 | Hamamatsu Photonics K.K. | Liquid treating method and liquid treating apparatus |
| CN2581058Y (en) * | 2002-08-29 | 2003-10-22 | 程树群 | Emulsifying device for iodipin and medicinal preparation |
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