CN106431695A - Lentinus edodes medium - Google Patents

Lentinus edodes medium Download PDF

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Publication number
CN106431695A
CN106431695A CN201610918096.9A CN201610918096A CN106431695A CN 106431695 A CN106431695 A CN 106431695A CN 201610918096 A CN201610918096 A CN 201610918096A CN 106431695 A CN106431695 A CN 106431695A
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parts
culture medium
sea duck
mushroom culture
pieris
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文雪妮
谭小红
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Guangxi Yulin Hexing Agricultural Technology Co Ltd
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Guangxi Yulin Hexing Agricultural Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F1/00Fertilisers made from animal corpses, or parts thereof
    • C05F1/002Fertilisers made from animal corpses, or parts thereof from fish or from fish-wastes
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Soil Sciences (AREA)
  • Plant Pathology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention provides a lentinus edodes medium. The lentinus edodes medium is mainly prepared from the following raw materials: cercis chinensis branches, fins, sea duck manure, pieris polita leaves, northern asarum, geranium carolinianum roots, puberulous glochidion herb, sea duck eggshells, alfalfa seeds and potassium fulvic acid. The preparation method specifically comprises the following steps: mixing the cercis chinensis branches, fins, sea duck manure, pieris polita leaves, northern asarum, geranium carolinianum roots, puberulous glochidion herb, sea duck eggshells and alfalfa seeds, spraying water until the moisture content is 60-70% during stirring, so as to obtain a mixture; composting the mixture, so as to obtain composted fermentation broth; uniformly stirring the obtained composted fermentation broth and the potassium fulvic acid, filling a polyethylene film charging barrel bag with the specification of 30*40*0.005cm, sterilizing, so as to obtain the lentinus edodes medium. The lentinus edodes medium prepared by the invention is capable of effectively killing insects and inhibiting bacteria, so that the yield of lentinus edodes is improved, and the quality is high.

Description

A kind of mushroom culture medium
【Technical field】
The invention belongs to culture medium of edible fungus field is and in particular to a kind of mushroom culture medium.
【Background technology】
China has abundant edible fungi species resource, traditional mode of production and modernization planting patternss and deposits, edible fungi yield Account for more than the 70% of Gross World Product, ranking in Chinese planting industry for its gross output value is only second to grain, cotton, oil, dish, really, occupy the Six.Except abundant wild mushroom resource, the cultivation species of edible fungi of china has reached kind more than 70, large kind have Lentinus Edodess, Pleurotus ostreatus, Auricularia, Agaricus Bisporus, Flammulina velutiper (Fr.) Sing, Volvariella volvacea (Bull.Ex Franch.) Singer. etc., a series of rare kind such as Pleurotus nebrodensis, Pleurotus eryngii, agrocyb eaegerita, Hypsizygus marmoreuss, ash tree Flower etc. is also favored by market, becomes edible fungi of china industry new growth point.
Lentinus Edodess as a kind of edible fungi, containing high protein, low fat, polysaccharide, several amino acids and multivitamin.Lentinus Edodess There are multiple medicinal functions, such as improve body's immunity, antioxidation, slow down aging, cancer-resisting, lowering blood pressure and blood fat and Cholesterol, Lentinus Edodess also have therapeutical effect to diabetes, pulmonary tuberculosis, infectious hepatitis, neuritiss etc., can be used for digestion not again Good, constipation etc..
Lentinus Edodess in incubation, because of place sanitary condition is poor, sterilizing not thoroughly, inoculation misoperation, packaging The reasons such as breakage, therefore, during Edible Fungi, the pollution of pest and disease damage is increasingly severe.Wherein, most common, be most serious By the pollution of fungus-caused compost and multiple fungal diseases, cause heavy losses to production, or even total crop failure.As Trichoderma viride just It is one of most common in edible fungus culturing, harm most serious pathogenic bacteria, it not only pollutes compost, causes strain or bacterium bag report Useless, also can cause parasitic disease on hypha of edible fungus and sporophore simultaneously.In cultivating in bag, insect is mainly mite class and line Worm, gnaws Lentinus Edodess, reduces yield.People are usually used Multiple Pesticides to prevent and treat fungal contamination, the fungal disease of edible fungi at present And insect, but due to limited conditions, in Edible Fungi, the phenomenon of pesticide abuse is fairly common.Pesticide residues excessively not only endanger Healthy, and directly influence the outlet of the edible fungus of China.Thus, find and a kind of there is antibacterial insecticidal function Mushroom culture medium becomes the new demand in edible fungi field.
【Content of the invention】
For deficiency of the prior art, it is an object of the invention to provide a kind of mushroom culture medium, this culture medium is antibacterial Good disinsection effect, mushroom production and quality all can be improved.
For reaching above-mentioned purpose, the present invention adopts the following technical scheme that:A kind of mushroom culture medium, described mushroom culture medium master Will be with branch of Lignum cercis chinensis, fin, sea duck excrement, pieris leaf, Herba Asari, wild geranium root, Fructus glochidionis puberi(Herba Glochidii Puberi), sea duck eggshell, alfalfa seed and Huang Rotten acid potassium is made up of raw material.
Further, described mushroom culture medium is mainly made up of the raw material of following weight portion:Branch of Lignum cercis chinensis 40-55 part, fin 7-10 part, sea duck excrement 10-18 part, pieris leaf 5-7 part, Herba Asari 4-6 part, wild geranium root 5-8 part, Fructus glochidionis puberi(Herba Glochidii Puberi) 5-10 part, Sea duck eggshell 5-10 part, alfalfa seed 4-7 part and potassium fulvate 1-3 part.
Further, described mushroom culture medium is mainly made up of the raw material of following weight portion:48 parts of branch of Lignum cercis chinensis, 9 parts of fin, 14 parts of sea duck excrement, 6 parts of pieris leaf, 5 parts of Herba Asari, wild 7 parts of geranium root, 8 parts of Fructus glochidionis puberi(Herba Glochidii Puberi), 8 parts of sea duck eggshell, alfalfa seed 6 Part and 2 parts of potassium fulvate.
Further, the preparation method of described mushroom culture medium comprises the following steps:
(1) pick described material on request;
(2) by branch of Lignum cercis chinensis, fin, sea duck excrement, pieris leaf, Herba Asari, wild geranium root, Fructus glochidionis puberi(Herba Glochidii Puberi), sea duck eggshell, lucerne Mu seed mixes, and spraying water while stirring to moisture content is 60%~70%, obtains compound;
(3) compost:1. build heap:By the compound of step (2) gained according to height:Wide:Long=1:1:2 require to stack, every 0.6~0.8m inserts wooden stick, builds after heap finishes, wooden stick is taken out, inserts and form passage at wooden stick, promote stockpile in stockpile Breathability;Stockpile covered rearing with plastic film, so that heat and moisture preserving, through two days later, opening heap top plastic sheeting, increases stockpile Aeration;2. turning:Daily thermometric once, starts mechanical turning when heap temperature rise is to 60 DEG C, turns over twice daily, heap temperature rise to 70 DEG C when daily turning three times, when compost produces white flock hair but during odorless abnormal smells from the patient, stack fermentation and complete, obtain compost fermentation thing;
(4) loading specification after mixing the compost fermentation thing of step (3) gained and potassium fulvate thoroughly is 30 × 40 × 0.005cm In polyethylene film barrel bag, under atmospheric pressure state, thing will be packed and sterilize in 110 DEG C of steam 12~24h, then be cooled to 25 ~30 DEG C, obtain final product mushroom culture medium.
Beneficial effects of the present invention:
In the present invention, branch of Lignum cercis chinensis contains cellulose, lignin, hemilignin etc., provides abundant carbon source for mushroom growth; Fin main component is protein, also contains crude protein, coarse ash, fat, saccharide, inorganic salt, vitaminss, is mushroom growth Abundant nitrogen source is provided;Sea duck excrement contains carbamide, uric acid and a small amount of vitamin, also contains a great number of elements calcium, phosphorus, magnesium and potassium simultaneously Deng;Pieris leaf has severe toxicity, and its water decoction can kill agricultural pests;The pure sun of Herba Asari great Xin, is suddenly brave product in medicine, can parasite killing, The varied bacteria growings such as aspergillus niger, rhizopus niger and Trichoderma spp. can be suppressed;The extract of wild geranium root contains flavonoid, catechin, list Rather, the duplication of multiple viruses can be suppressed, the effect of wherein resisiting influenza virus is the most obvious, it is possible to decrease the sense of various Influenza virus strain Metachromia, it is also possible to the growth of suppression S. aureus in vitro and Candida albicans, has antibacterial and sterilizing effect;Folium glochidionis puberi Other dysentery bacteriums (Shu Shi, Freund, Song Shi) and Salmonella (typhoid fever, paratyphoid fever, hog cholera, bacillus enteritidiss) are had necessarily Antibacterial action, Herb can parasite killing;Sea duck eggshell is mainly existed with calcium carbonate form, also calcium, phosphorus, ferrum, zinc, iodine, magnesium, potassium, selenium Deng multiple element and 10 multivitamins;Alfalfa seed mainly contains carbohydrate, protein and dietary fiber, also contains thiamine The element such as element, riboflavin, retinol and calcium, magnesium, ferrum, manganese, zinc, copper, potassium, phosphorus, sodium, selenium, seeks containing necessary to Lentnus edodes Foster material;Potassium fulvate can improve the permeability of cell, the more conducively absorption to nutrient for the Lentinus Edodess, improves the utilization of culture medium nutrient Rate, reaches the purpose of volume increase.
In sum, advantage of the present invention has:(1) culture medium of present invention preparation greatly reduces Pesticide use amount, can have Effect parasite killing and suppression miscellaneous bacteria, reduce pesticide dosage, pollution-free to environment and edible fungi, protect ecological environment, promote the well-being of mankind again, It is the general orientation of following edible fungi development;(2) comprehensively, concentration is high for this product nutrient, and mushroom production significantly improves, and quality is more excellent.
【Specific embodiment】
The following examples can help those skilled in the art to be more completely understood by the present invention, but cannot be with any Mode limits the present invention.
Embodiment 1:
A kind of mushroom culture medium, is mainly made up of the raw material of following weight portion:40 parts of branch of Lignum cercis chinensis, 7 parts of fin, sea duck excrement 10 Part, 5 parts of pieris leaf, 4 parts of Herba Asari, wild 8 parts of geranium root, 10 parts of Fructus glochidionis puberi(Herba Glochidii Puberi), 10 parts of sea duck eggshell, 7 parts of alfalfa seed and Huang Rotten 3 parts of potassium of acid.Concrete preparation method comprises the following steps:
(1) pick described material on request;
(2) by branch of Lignum cercis chinensis, fin, sea duck excrement, pieris leaf, Herba Asari, wild geranium root, Fructus glochidionis puberi(Herba Glochidii Puberi), sea duck eggshell, lucerne Mu seed mixes, and spraying water while stirring to moisture content is 60%~70%, obtains compound;
(3) compost:1. build heap:By the compound of step (2) gained according to height:Wide:Long=1:1:2 require to stack, every 0.6~0.8m inserts wooden stick, builds after heap finishes, wooden stick is taken out, inserts and form passage at wooden stick, promote stockpile in stockpile Breathability;Stockpile covered rearing with plastic film, so that heat and moisture preserving, through two days later, opening heap top plastic sheeting, increases stockpile Aeration;2. turning:Daily thermometric once, starts mechanical turning when heap temperature rise is to 60 DEG C, turns over twice daily, heap temperature rise to 70 DEG C when daily turning three times, when compost produces white flock hair but during odorless abnormal smells from the patient, stack fermentation and complete, obtain compost fermentation thing;
(4) loading specification after mixing the compost fermentation thing of step (3) gained and potassium fulvate thoroughly is 30 × 40 × 0.005cm In polyethylene film barrel bag, under atmospheric pressure state, thing will be packed and sterilize in 110 DEG C of steam 12~24h, then be cooled to 25 ~30 DEG C, obtain final product mushroom culture medium.
Embodiment 2:
A kind of mushroom culture medium, is mainly made up of the raw material of following weight portion:48 parts of branch of Lignum cercis chinensis, 9 parts of fin, sea duck excrement 14 Part, 6 parts of pieris leaf, 5 parts of Herba Asari, wild 7 parts of geranium root, 8 parts of Fructus glochidionis puberi(Herba Glochidii Puberi), 8 parts of sea duck eggshell, 6 parts of alfalfa seed and yellow humic 2 parts of sour potassium.Concrete preparation method is same as Example 1.
Embodiment 3:
A kind of mushroom culture medium, is mainly made up of the raw material of following weight portion:55 parts of branch of Lignum cercis chinensis, 10 parts of fin, sea duck excrement 18 parts, 7 parts of pieris leaf, 6 parts of Herba Asari, wild 5 parts of geranium root, 5 parts of Fructus glochidionis puberi(Herba Glochidii Puberi), 5 parts of sea duck eggshell, 4 parts of alfalfa seed and Huang Rotten 1 part of potassium of acid.Concrete preparation method is same as Example 1.
Comparative example 1:
A kind of mushroom culture medium, is mainly made up of the raw material of following weight portion:48 parts of branch of Lignum cercis chinensis, 9 parts of fin, sea duck excrement 14 Part, 8 parts of sea duck eggshell, 6 parts of alfalfa seed and 2 parts of potassium fulvate, concrete preparation method is same as Example 1.
Comparative example 2:
Conventional mushroom culture medium.
By the pack of the mushroom culture medium of embodiment 1-3, comparative example 1 and comparative example 2 gained, inoculation, every kind of fill 15 bags, every bag Weight 0.9kg.To all participate in contrast tests edible fungi carry out Routine Management, and record mycelial growth rate, fruiting yield and Living contaminantses situation, to measure application effect in cultivating champignon for the culture medium of the present invention.Be Mucor racemosus Fres for trying mold species, Trichoderma viride, aspergillus niger, bread mold, penicillium sp, are culture environment identical quality xianggu strain for examination edible mushroom strainses.Various trainings Application effect in cultivating champignon for the foster base is as shown in table 1.
Application effect table in cultivating champignon for the various culture medium of table 1
Title Mycelial growth rate (mm/d) Fruiting yield (g/bag) Contaminate miscellaneous rate (%)
Embodiment 1 5.31 178.35 3.13
Embodiment 2 5.36 181.34 2.68
Embodiment 3 4.95 177.55 3.21
Comparative example 1 4.92 158.73 14.13
Comparative example 2 4.68 154.68 14.25
As can be seen from the above table, embodiment 1-3 mycelial growth rate, fruiting yield are all high than comparative example, and contaminate miscellaneous rate Well below comparative example;From embodiment 2 and comparative example 1 as can be seen that pieris leaf, Herba Asari, wild geranium root, Fructus glochidionis puberi(Herba Glochidii Puberi) have The effect of certain killing pests and suppressing bacteria.So, using the culture medium of the present invention, can parasite killing and suppression varied bacteria growing, mushroom production is notable Improve, quality is more excellent.
Although, above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.

Claims (4)

1. a kind of mushroom culture medium it is characterised in that:Described mushroom culture medium is mainly with branch of Lignum cercis chinensis, fin, sea duck excrement, pieris Leaf, Herba Asari, wild geranium root, Fructus glochidionis puberi(Herba Glochidii Puberi), sea duck eggshell, alfalfa seed and potassium fulvate are made up of raw material.
2. mushroom culture medium as claimed in claim 1 it is characterised in that:Described mushroom culture medium is mainly by following weight portion Raw material is made:Branch of Lignum cercis chinensis 40-55 part, fin 7-10 part, sea duck excrement 10-18 part, pieris leaf 5-7 part, Herba Asari 4-6 part, open country Geranium root 5-8 part, Fructus glochidionis puberi(Herba Glochidii Puberi) 5-10 part, sea duck eggshell 5-10 part, alfalfa seed 4-7 part and potassium fulvate 1-3 part.
3. mushroom culture medium as claimed in claim 2 it is characterised in that:Described mushroom culture medium is mainly by following weight portion Raw material is made:48 parts of branch of Lignum cercis chinensis, 9 parts of fin, 14 parts of sea duck excrement, 6 parts of pieris leaf, 5 parts of Herba Asari, wild 7 parts of geranium root, calculation 8 parts of plate, 8 parts of sea duck eggshell, 6 parts of alfalfa seed and 2 parts of potassium fulvate.
4. described mushroom culture medium as arbitrary in claim 1-3 it is characterised in that:The preparation method bag of described mushroom culture medium Include following steps:
(1) pick described material on request;
(2) by branch of Lignum cercis chinensis, fin, sea duck excrement, pieris leaf, Herba Asari, wild geranium root, Fructus glochidionis puberi(Herba Glochidii Puberi), sea duck eggshell, alfalfa seed Mixing, spraying water while stirring to moisture content is 60%~70%, obtains compound;
(3) compost:1. build heap:By the compound of step (2) gained according to height:Wide:Long=1:1:2 require to stack, every 0.6~ 0.8m inserts wooden stick, builds after heap finishes, wooden stick is taken out, inserts and form passage at wooden stick in stockpile, promote the ventilative of stockpile Property;Stockpile covered rearing with plastic film, so that heat and moisture preserving, through two days later, opening heap top plastic sheeting, increases the ventilation of stockpile Property;2. turning:Daily thermometric once, starts mechanical turning when heap temperature rise is to 60 DEG C, turns over twice daily, when heap temperature rise is to 70 DEG C Daily turning three times, when compost produces white flock hair but during odorless abnormal smells from the patient, stacks fermentation and completes, obtain compost fermentation thing;
(4) loading specification after mixing the compost fermentation thing of step (3) gained and potassium fulvate thoroughly is the poly- second of 30 × 40 × 0.005cm In alkene thin film barrel bag, under atmospheric pressure state, thing will be packed and sterilize in 110 DEG C of steam 12~24h, then be cooled to 25~30 DEG C, obtain final product mushroom culture medium.
CN201610918096.9A 2016-10-20 2016-10-20 Lentinus edodes medium Withdrawn CN106431695A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103724114A (en) * 2013-12-12 2014-04-16 颍上县鸿涛菌业专业合作社 Shiitake cultivation material using orange branches as raw material and preparation method thereof
CN103739364A (en) * 2013-12-12 2014-04-23 颍上县鸿涛菌业专业合作社 Mushroom cultivation material by taking apple branch as raw material and preparation method thereof
CN103880546A (en) * 2014-03-26 2014-06-25 泗阳县农业科学研究所 Lentinula edodes culturing base stock cultivated by utilizing pleurotus ostreatus and preparation method thereof
CN104945129A (en) * 2015-06-18 2015-09-30 曾少兰 Mushroom culture medium
CN105613047A (en) * 2016-02-26 2016-06-01 云南神奕农业科技有限公司 Planting method of Letinus edodes

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103724114A (en) * 2013-12-12 2014-04-16 颍上县鸿涛菌业专业合作社 Shiitake cultivation material using orange branches as raw material and preparation method thereof
CN103739364A (en) * 2013-12-12 2014-04-23 颍上县鸿涛菌业专业合作社 Mushroom cultivation material by taking apple branch as raw material and preparation method thereof
CN103880546A (en) * 2014-03-26 2014-06-25 泗阳县农业科学研究所 Lentinula edodes culturing base stock cultivated by utilizing pleurotus ostreatus and preparation method thereof
CN104945129A (en) * 2015-06-18 2015-09-30 曾少兰 Mushroom culture medium
CN105613047A (en) * 2016-02-26 2016-06-01 云南神奕农业科技有限公司 Planting method of Letinus edodes

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Application publication date: 20170222