CN106418496A - Process for producing instant kudzuvine root powder by use of enzymolysis method - Google Patents
Process for producing instant kudzuvine root powder by use of enzymolysis method Download PDFInfo
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Abstract
The invention mainly researches a process for producing instant kudzuvine root powder by use of enzymolysis method. The process comprises the following steps of: measuring and researching factors which influence the viscosity by use of a rheometer; then inspecting influence of factors such as enzyme amount, hydrolysis time and hydrolysis temperature for kudzuvine root powder hydrolysis on instant dissolution effect, and determining optimal conditions for the process through an orthogonal test; and finally, measuring various physical and chemical properties of the instant kudzuvine root powder, including moisture content, reducing sugar content, total sugar content, starch granule structure and flavonoid content, wherein according to the result, the optimal conditions for the process for preparing the instant kudzuvine root powder are as follows: the enzyme amount is 0.8g, the gelatinization temperature is 60 DEG C, the gelatinization time is 10 minutes, and the drying time is 19h. Under such optimal process conditions, the product dissolubility is increased, the instant dissolution is improved, observation can be realized by use of a scanning electron microscope (SEM), most of instant kudzuvine root powder particles are in an irregular polygon shape and have shrunk surfaces, and particle distribution is relatively loose.
Description
Technical field
The present invention provides a kind of technique of Production by Enzymes distant Radix Puerariae powder, belongs to functional food preparation field.
Background technology
Powder of Radix Puerariae, is the starch extracting in pulse family Pueraria Radix Puerariae, is the medicine food common source that China's Ministry of Public Health is announced.
It is《China's book on Chinese herbal medicine》The medical herbs recording, medicinal source is legume pueraria lobata, the tuber of Radix Puerariae rattan.Wild authentic Radix Puerariae, outward
Face has one layer of oxide.Radix Puerariae starch and kudzuvine root total powder can be divided into, both differences are that processing technique is different, medicinal ingredient
Content is different.Radix Puerariae includes 12% flavone compound, such as the nutritional labeling such as puerarin, daizeol glycosides, peanut element, also
Protein, aminoacid, sugar and the mineral such as ferrum needed by human, calcium, copper, selenium, are all-ages famous and precious tonic, have " thousand
The good reputation of year Radix Ginseng ".Existing medical value, and the effect of nutritious health care.Mainly originate in Hubei, Anhui, Guizhou, Guangdong, wide
The ground such as west, Jiangsu, Jiangxi, Sichuan, Chongqing, Hunan.
While people gradually recognize pueraria root health care and pharmacological function, also more and more stronger to the attention rate of Radix Puerariae abroad
Strong.Abroad, some western developed country creative utilization Radix Puerariaes produce kudzu root oral liquid, Pueraria lobota freezes canned food, Radix Puerariae melange etc.
Best-selling product.Also broadly fall into best seller always in Japanese kudzu root foods, wherein produced Pueraria DC beverage, noodles, vermicelli,
The daily food such as ice cream and bread.More even, kudzu root foods are listed in the specially offered product of imperial family of Japan, but due to resource
Deficient and for the protection to national natural resourcess and ecological environment, Japan throughout the year in a large number cheap from China, Thailand etc.
Ground import Radix Puerariae raw material, transports this country back and is processed on finished product input market both domestic and external.
Content of the invention
The purpose of the present invention is to change molecular structure and the physicochemical characteristic of Amylum Puerariae Radicis by enzymatic isolation method appropriateness, optimizes enzyme process system
The technique of standby instant kudzu vine powder, and have detected the moisture of instant kudzu vine powder, total sugar, reducing sugar, the content property indices of flavone：I.e.
Observe the starch structure of Radix Puerariae by scanning electron microscope sem, compared for digesting instant kudzu vine powder structure after front Amylum Puerariae Radicis and enzymolysis；Then adopt
With DPPH method and Fe3+Reduction force method is furtherd investigate to the antioxidant activity of Amylum Puerariae Radicis extract, and is made using ascorbic acid
For positive control.Exploitation for the functional food of distant Radix Puerariae powder provides related data.Concrete technology is as follows：
(1) clean Radix Puerariae is cut into 2-3 centimetre of segment, adds water, pulverized pulping, Radix Puerariae and water with soy bean milk making machine
Mass ratio be 1：3-5, after pulverizing, carries out extruding and filters, filtrate is stood 48h, after its layering, in siphon with two-layer gauze
Layer liquid, and lower sediment is placed in 24h in 50 DEG C of baking ovens, until drying, the solid after drying is broken into pieces, continues in an oven to dry
Dry 24h, the solid pulverizer of moisture drying completely is ground into powder, that is, obtains powder of Radix Puerariae, be sealed against, in 4 DEG C of refrigerators
Middle stored refrigerated；
(2) configuration quality concentration is the powder of Radix Puerariae solution of 30-60%, adds amylase, and diastatic addition is Radix Puerariae
The 1-5% of grain weight amount, after gelatinizing 10-60min in 35 DEG C -65 DEG C of water bath with thermostatic control, being placed in 18h-45h in 50 DEG C of baking oven is
Distant Radix Puerariae powder can be obtained.
In step (2), under optimum condition, powder of Radix Puerariae concentration of polymer solution is 50%, and added amylase diastatic adds
Dosage is the 4% of powder of Radix Puerariae weight, after gelatinizing 20min in 50 DEG C of water bath with thermostatic control, is placed in 22h in 50 DEG C of baking oven
Prepared distant Radix Puerariae powder.
13.123% is reached containing moisture in Amylum Puerariae Radicis；In instant kudzu vine powder, content of reducing sugar is 4.49%, and total sugar content is
39.1%, wherein total sugar content is apparently higher than reducing sugar；Using 70% ethanol as extractant, using ultrasonic wave extraction from
Extract in 50g Amylum Puerariae Radicis and obtain crude flavonoid powder, its extraction ratio is 2.38%；The content of total flavones in measuring Amylum Puerariae Radicis, result is 50g
Containing flavone quality in Amylum Puerariae Radicis is 21.525mg；The observation of Amylum Puerariae Radicis grain structure before and after enzymolysis, starch particle shapes before and after enzymolysis
All assume irregular polygon, without enzymolysis Amylum Puerariae Radicis particle surface is smooth, flawless, distribution of particles are intensive, and after digesting
Instant kudzu vine powder granule, surface shrinkage, little particle quantity reduces relatively, and distribution of particles is more loose.
Brief description
Fig. 1 is the process chart of Production by Enzymes distant Radix Puerariae powder.
Fig. 2 is the impact to powder of Radix Puerariae viscosity for the different heating time.
Fig. 3 is the impact to powder of Radix Puerariae viscosity for the different powder of Radix Puerariae concentration.
Fig. 4 is the impact to powder of Radix Puerariae viscosity for the different shear rates.
Fig. 5 is glucose standard curve.
Fig. 6 is the impact to powder of Radix Puerariae hydrolysis degree for the different enzyme dosages.
Fig. 7 is the impact to powder of Radix Puerariae hydrolysis degree for the differential responses temperature.
Fig. 8 is the impact to powder of Radix Puerariae hydrolysis degree for the differential responses time.
Fig. 9 is the outside drawing of powder of Radix Puerariae after amylase before processing, and wherein A is amylase before processing powder of Radix Puerariae；B is amylase
Powder of Radix Puerariae after process.
Figure 10 is instant capacity figure after amylase before processing, and wherein, A is amylase before processing powder of Radix Puerariae；B is at amylase
Powder of Radix Puerariae after reason.
Figure 11 is powder of Radix Puerariae and ascorbic acid compares to the clearance rate of DPPH.
Figure 12 is the grain structure contrast of Amylum Puerariae Radicis before and after enzymolysis under 5000 times of Electronic Speculum, before A is enzymolysis；After B is enzymolysis.
Figure 13 is that the grain structure of Amylum Puerariae Radicis compares, before A is enzymolysis before and after enzymolysis under 2000 times of Electronic Speculum；After B is enzymolysis.
Specific embodiment
Experimental drug
α-amylase, glucose, 3,5- dinitrosalicylic acids, sodium hydroxide, sodium potassium tartrate tetrahydrate, anhydrous calcium chloride, rutin,
DPPH, the potassium ferricyanide, trichloroacetic acid.
Experimental apparatus
SP-754 ultraviolet-uisible spectrophotometer, Shanghai Spectrum Apparatus Co., Ltd.；Electronic balance ME204E, Bo Tele-
Support benefit instrument (Shanghai) Co., Ltd.；Flow graph；Far infrared Quick drying box, Shanghai leap Medical Devices Co., Ltd.；Number
Aobvious thermostat water bath HH-2, Changzhou Zhi Borui instrument manufacturing company limited；Pulverizer, Tianjin Stettlen Instrument Ltd.；
RE-5285A rotary evaporator, Shanghai Yarong Biochemical Instrument Plant；Scanning electron microscope sem, NEC jeol-7500f.
Amylum Puerariae Radicis make
Raw material is chosen：It is preferred in Qiu Mo with the beginning of the winter with more than 3 years persons of growth, because experiment has limitation so this experiment is selected
Take fresh Radix Puerariae, remove clean water 2 times after soil, clean silt debris with brush.Clean Radix Puerariae is cut into 2-3 centimetre little
Section.According to solid-liquid ratio 1：4 ratio that adds water, is pulverized pulping with soy bean milk making machine.After pulverizing, carry out extruding with two-layer gauze
Filter, filtrate is stood 48h, after its layering, siphon supernatant liquid, and lower sediment is placed in 24h in 50 DEG C of baking ovens, until drying
Dry.Solid after drying is broken into pieces, continues in an oven to dry (24h).The solid pulverizer of moisture drying completely is pulverized
Become powder, that is, obtain powder of Radix Puerariae.It is sealed against, stored refrigerated in 4 DEG C of refrigerators.
The mensure of impact viscosity considerations
The impact to solution viscosity for the different heating time：Prepare five groups of the Amylum Puerariae Radicis solution of 4g/100ml, boiling water bath adds respectively
Hot 20min, 30min, 40min, 50min, 60min.It is cooled to 25 DEG C, its viscosity is surveyed with 60r/min mixing speed.
The impact of different solutions concentration on viscosity：Respectively prepare 4g/100ml, 5g/100ml, 6g/100ml, 7g/100ml,
8g/100ml Amylum Puerariae Radicis solution.Boiling water bath heats 20min, is cooled to 25 DEG C, surveys its viscosity with 60r/min mixing speed.
The impact to viscosity for the different shear rates：Prepare the Amylum Puerariae Radicis solution of 4g/100ml, boiling water bath heats 20min, cooling
To 25 DEG C, respectively its viscosity is surveyed with the mixing speed of 15r/min, 30r/min, 40r/min, 50r/min, 60r/min.
Determination of Reducing Sugars
(1) preparation of glucose standards solution
Accurately weigh the pure glucose 100mg of analysis, be settled to 100mL with after a small amount of distillation water dissolution, be configured to quality
Concentration is the glucose standards solution of 1mg/mL, saves backup.
(2) drafting of glucose standard curve
Glucose standard curve：Take the colorimetric test tube of 9 brace plugs, the order pressing table respectively adds various reagents.By each examination
Agent sequentially add and mix homogeneously after, heat 5min in boiling water bath, cooled down with flowing water immediately after, each test tube adds distilled water extremely
25ml, is sufficiently mixed uniformly.With the zeroing of blank tube solution, at 540nm, measure light absorption value with ultraviolet spectrophotometer.
(3) sample treatment
Accurately weigh 2.0g Amylum Puerariae Radicis, be placed in 100mL beaker or conical flask, add 40mL distilled water, mix；Beaker is put
It is incubated 20min in 50 DEG C of water-baths, frequently stir；Take out beaker, beaker contents are proceeded to the volumetric flask of a 100mL
In, add water constant volume.It is sufficiently mixed, filters, filter liquor is used for measuring reducing sugar.
(4) reducing sugar test
Color comparison tube is numbered, is separately added into reagent；It is separately added into DNS reagent 1.5mL in above-mentioned color comparison tube, fully mixed
Close；Then in boiling water bath, heating 5min is developed the color；Each pipe is put into the beaker cooling filling cold water, add distilled water to each pipe
To each color comparison tube 25mL graduation mark, shake up；Do blank with No. 1 pipe, measure often absorbance value at 540nm wavelength for the pipe,
Record result；According to the OD value of reducing sugar and total sugar, using the standard working curve of glucose, calculate content of reducing sugar.
Single factor experiment
Starch Hydrolysis single factor experiment
The impact to hydrolysis for the different enzyme additions：Adjust Amylum Puerariae Radicis breast concentration 50% (20g Amylum Puerariae Radicis, 40ml water), add respectively
0.2g, 0.4g, 0.6g, 0.8g, 1.0g amylase, in 50 DEG C of thermostat water bath gelatinizing 20min, puts into drying baker and is dried.
The impact to hydrolysis for the different gelatinization points：Adjust Amylum Puerariae Radicis breast concentration 50% (20g Amylum Puerariae Radicis, 40ml water), add 0.8g
Amylase, respectively in 35 DEG C, 45 DEG C, 50 DEG C, 55 DEG C, 65 DEG C of thermostat water bath gelatinizing 20min, puts into drying baker and is dried.
The impact to hydrolysis for the different gelatinization times：Adjust Amylum Puerariae Radicis breast concentration 50% (20g Amylum Puerariae Radicis, 40ml water), add 0.8g
Amylase, respectively in 50 DEG C of thermostat water baths gelatinizing 10min, 20min, 30min, 40min, 50min, 60min, puts into drying baker
It is dried.
The impact to hydrolysis for the different drying times：：Adjust Amylum Puerariae Radicis breast concentration 50% (20g Amylum Puerariae Radicis, 40ml water), add 0.8g
Amylase, in 50 DEG C of thermostat water bath gelatinizing 20min, different drying times are dried.
Sample treatment
Dried sample is put into pulverizer smash, put into plastic packaging bag and save backup.
The determination of optimum process condition
On the basis of single factor experiment, each factor chooses 3 levels, using L9(34) table makees orthogonal test.The orthogonal reality of table 1
Test factor level
Table 1
Instant capacity is tested
Moistening sinking is tested
Accurately weigh 10g Amylum Puerariae Radicis, be dispersed on 25 DEG C of waters surface of 250mL, respectively under conditions of standing and stirring, measure
The time that the whole moistening of Amylum Puerariae Radicis is sunk.
The mensure of instant capacity
With (80 DEG C) dissolving 5g powder of 45g distilled water, start agitator stirring at low speed, the time that record powder is completely dissolved
(s).According to the instant standard of food industry, all the time of dissolving is as instant in 5s.
The mensure of instant kudzu vine powder moisture
After drying, the quality of instant kudzu vine powder sample loss is exactly moisture.Using 105 DEG C of oven method measurings：First will clean
Glass drying oven dry 90min at 105 DEG C, after adding a cover, in exsiccator, be cooled to room temperature, be precisely weighed.Add sample, record
Sample quality m0, in 105 DEG C of baking ovens, then dry 90min, taking-up is added a cover, and cools down, weigh in exsiccator.Dry 30min again, take
Go out cooling to weigh.So repeatable operation, until constant weight, sample quality m after record drying1.It is calculated as follows moisture.
Moisture (%)=(m0-m1)×100/m0
m0:Example weight (g)；m1：Weight (g) after sample drying.
The mensure of general flavone content in Amylum Puerariae Radicis
(1) drafting of rutin standard curve
Accurately weigh control substance of Rutin 10mg being dried under the conditions of 105 DEG C to constant weight, add 95% ethanol to dissolve simultaneously
It is settled to 10ml, be made into the rutin standard solution that concentration is 1mg/ml, standby as prepare liquid.
Precision measure rutin standard solution 0,0.1,0.2,0.3,0.4,0.5,0.6ml, every test tube all adds water to
3ml.It is subsequently adding 0.5ml 5% sodium nitrite in aqueous solution, after standing 6min, add the aluminum nitrate solution of 0.5ml 10%, mix
Close and uniformly place 6min afterwards, add 5% sodium hydroxide solution, after mixing, place 15min.Then it is settled to distilled water
10ml, measures light absorption value with ultraviolet spectrophotometer after mixing at 500nm wavelength.According to the data obtained, with rutin quality it is
Abscissa, absorbance is vertical coordinate, draws rutin standard curve.
(2) in Amylum Puerariae Radicis sample general flavone content mensure
Take above-mentioned ultrasonic extract 1ml, draw operation by rutin standard curve and carry out, measure its light absorption value, and by institute
The standard curve obtaining calculates the content of total flavones.
The mensure of Amylum Puerariae Radicis extract antioxygenic property
(1) mensure to DPPH Scavenging activity
Accurately weigh dried testing sample (instant kudzu vine powder extract) and ascorbic acid sample 50mg in an oven, point
Not with distilling water dissolution and being settled to 25mL, make the solution that mass concentration is 2mg/mL.Accurately weigh 6.3mg
DPPH powder, in brown bottle, adds 100ml anhydrous alcohol solution, is configured to the DPPH mother solution of 0.16mmol/L, places
Save backup in the dark.
(2) mensure of reduced iron ion energy
Accurately weigh 0.6g testing sample, with distillation water dissolution and be settled to 100ml and (a small amount of solid may be contained after dissolving
Powder, available filter paper filtering), it is made into the sample solution that mass concentration is 4mg/mL, be then diluted with distilled water into different dense
Degree gradient.Measure the sample solution of 2.5mL variable concentrations, add the phosphate buffer (pH=6.6) of 2.5mL 0.2mol/L with
And the potassium ferricyanide aqueous solution of 2.5mL 1%, 50 DEG C of water-bath reacts 20min, is then cooled down with flowing water rapidly, add
The solution of trichloroacetic acid of 2.5mL 10%, to be mixed uniformly after extract reaction solution 5mL, add the distilled water of 5mL and 0.1%
FeCl3Solution, mixes and reacts after 10min after it, with water as blank, measures its light absorption value at 700nm.The light absorption value of solution
Bigger, indicate that its reducing power is bigger.Above experiment all does positive control with the VC of equal in quality concentration.
SEM observes the grain structure of Amylum Puerariae Radicis
Weigh a small amount of cross instant kudzu vine powder in the little centrifuge tube of 1.5mL without enzymolysis processing, add a small amount of nothing
Water-ethanol dissolves, by supersonic oscillations 3-5 minute so as to abundant dissolve.Actual experiment operating process weighs 0.04g Amylum Puerariae Radicis,
It is subsequently adding 0.8mL anhydrous alcohol solution.Instant kudzu vine powder processing procedure after enzymolysis is ibid.
Draw Amylum Puerariae Radicis solution on the SEM sample stage posting tinfoil with capillary pipette, so that sample is sprawled in monolayer, so as far as possible
Metal spraying or spray carbon (thickness about 100 à) afterwards, observes (accelerating potential 20kV) enzymolysis Amylum Puerariae Radicis surface texture with scanning electron microscope sem, and with
Raw material Amylum Puerariae Radicis compare.
The factor of impact viscosity
The impact to viscosity for the different heating time
Be can be seen that by table 2 and Fig. 2：With the increase of heat time heating time, viscosity does not become regular change, but from data
As can be seen that viscosity does not have too big change, so heat time heating time does not have too much influence to the change of viscosity.
Table 2 heat time heating time of the impact to viscosity
The impact to viscosity for the variable concentrations
Be can be seen that by table 3 and Fig. 3：Increase viscosity with Amylum Puerariae Radicis concentration also increases as, and excursion is very big,
The concentration on viscosity impact of this explanation solution is very big, and the bigger viscosity of concentration is bigger.
The impact of table 3 concentration on viscosity
The impact to viscosity for the different shear rates
Be can be seen that by table 4 and Fig. 4：With the increase of shear rate, viscosity journey downward trend, it can be seen that shearing
Speed is inversely proportional to viscosity.
The impact to viscosity for table 4 shear rate
Single factor experiment
The impact to hydrolysis degree for the enzyme dosage
Shown in Fig. 6：Under experimental condition, when enzyme dosage is less than 0.8g, the enzyme unsaturation in substrate, content of reducing sugar gradually increases
Plus.When enzyme dosage is for 0.8g, the enzyme in substrate reaches saturation, and content of reducing sugar reaches maximum, hydrolysis degree highest.Thereafter,
Increase enzyme dosage, Changes of Reducing Sugar Content is inconspicuous, but on a declining curve.
The impact to hydrolysis degree for the reaction temperature
As shown in Figure 7：Under experimental condition, diastatic optimal activity temperature is about 60 DEG C.When temperature is less than 60 DEG C, enzyme
The rising of activity with temperature and be gradually increased, the hydrolysis degree of system is gradually increased, and content of reducing sugar is continuously increased；When temperature surpasses
When crossing 60 DEG C, enzyme part will lose activity because temperature is too high, leads to the hydrolysis degree of system to reduce, content of reducing sugar drops
Low.When temperature is higher than 75 DEG C, the most of inactivation of enzyme, hydrolysis degree is preferably minimized, and the reducing power of system no longer changes.
The impact to hydrolysis degree for the response time
As seen from Figure 8：Under experimental condition, the content of reducing sugar of system increases with the prolongation in response time, i.e. system
Hydrolysis degree constantly increases.After reaching 30min between when reacted, the content of reducing sugar journey downward trend of system, this shows：Suitably
Response time should be in 30min.
Orthogonal experiment results and analysis
Around the relatively suitable level of single factor experiment, each factor level choosing orthogonal test is as shown in table 1.
According to table 2 test data analyzer result：The primary and secondary order of factor is enzyme dosage → response time → reaction temperature；?
Excellent horizontal combination is enzyme amount 0.8g, 60 DEG C of reaction temperature, response time 10min.The sample reducing sugar obtaining under this process conditions
Content highest；Reconstitute sample with 90 DEG C of water, compare with commercially available instant kudzu vine powder, become paste uniformly, no grumeleuse, color and luster is sparkling and crystal-clear, quality
Be improved significantly.
Table 5 orthogonal experiments
According to orthogonal experiments, choose optimum condition and do three groups of parallel tests
Table 6 parallel test
Instant capacity is analyzed
Moistening sinking is tested
The time measuring full powder whole moistening sinking is respectively 200s and 8s, and the moistening of this explanation Amylum Puerariae Radicis is sunk relatively slowly, speed
Dissolubility is not fine.Amylum Puerariae Radicis moisten time of sinking and are respectively 182s and 4s after treatment, Amylum Puerariae Radicis after treatment heavy
Shallow lake sinking is improved.
Instant capacity
Accurately weigh the full powder of 5g Amylum Puerariae Radicis, reconstituted with 80 DEG C of water of 40mL, the situation that reconstitutes observed after agitation is：Have after reconstituting
A small amount of agglomerate, cup bottom is no precipitated.Place solution jaundice after a period of time.This explanation sweet potato whole powder can dissolve, but still has group
Block, shows that its instant capacity is not fine, needs to be improved.After Amylum Puerariae Radicis agitation after treatment, the situation that reconstitutes of observation is：Punching
After tune, agglomerate significantly reduces, and cup bottom is no precipitated, and places a period of time solution or milky.Amylum Puerariae Radicis instant capacity after explanation process
It is obviously improved.
As can be seen from Figure 9 through enzymatic treatment after Amylum Puerariae Radicis compare whiter before process, after Figure 10 finds out process
Placement for a period of time or milky.
The mensure of instant kudzu vine powder moisture
Weigh instant kudzu vine powder sample 5g, after drying to constant weight at 105 DEG C, weigh, can be calculated Amylum Puerariae Radicis moisture is
13.123%.
The mensure of general flavone content in instant kudzu vine powder
Take 1mL Amylum Puerariae Radicis ultrasonic extraction thing, carry out according to operation under rutin standard curve, recording its light absorption value is 0.087,
According to flavone concentration computing formula in sample：X=(Y+0.0211)/10.31, in calculating sample solution, the concentration of flavone is
0.0105mg/mL, contains flavone 0.105mg in 1mL instant kudzu vine powder extracting solution.Known Amylum Puerariae Radicis are through ultrasonic extraction and mistake
After filter, extraction liquor capacity is 205mL, and calculate extraction in 50g Amylum Puerariae Radicis further to obtain flavone quality is 21.525mg.
The result of study of antioxidant activity
(1) Amylum Puerariae Radicis extract removes DPPH Experiment on Function
This problem measure the scavenging action to DPPH for the Amylum Puerariae Radicis extract, and done with the ascorbic acid of equal in quality concentration right
According to as shown in Figure 11, in concentration range, Amylum Puerariae Radicis extract has stronger scavenging action to DPPH, and effect degree is with concentration
Increase and be gradually increased.Although still increasing with concentration to the clearance rate of DPPH after the concentration of sample reaches to a certain degree,
But amplification gradually slows down.Meanwhile, in this concentration range, ascorbic acid is in higher on the whole to the scavenging action of DPPH
Level.After the mass concentration of VC reaches 0.2mg/mL, its Scavenging activity nearly reaches top level and trend also tends to gently, its
Middle maximal clearance is 94.91%.
(2) test of Amylum Puerariae Radicis extract reduced iron ion energy
As shown in Figure 12, extract reduces Fe to the ability of Amylum Puerariae Radicis extract and ascorbic acid reduction iron ion3+Ability with
The increase of concentration and increase, and ascorbic acid reducing power maintains higher level, and the reducing power of ascorbic acid is substantially strong
In Amylum Puerariae Radicis extract.Extract and ascorbic acid are to Fe as can be seen from Table 53+Ability have notable difference.
The observation of Amylum Puerariae Radicis grain structure before and after enzymolysis
Scanning electron microscope sem observe instant kudzu vine powder enzymolysis before and after structure as shown in Figure 12,13, according to following two groups of pictures pair
The similarities and differences than Radix Puerariae starch grain structure before and after enzymolysis.Something in common：Before and after enzymolysis Radix Puerariae starch granule all present irregular
Polygon, flawless, granule size is uneven, and volume difference is larger.Difference：Amylum Puerariae Radicis granule before enzymolysis, surface light
Sliding, closely, its small particles number is more relative to bulky grain for starch granuless distribution, is tightly distributed round bulky grain.Speed after enzymolysis
Molten Amylum Puerariae Radicis granule, rough, starch granuless distribution is more loose on the whole, and its small particles number compares bulky grain relatively
Few.
Two groups of picture comparing result analyses understand, α-amylase is mainly the starch granuless decomposing small volume in Amylum Puerariae Radicis,
Most of bulky grains are remained, after this just makes enzymolysis, the starch granuless of instant kudzu vine powder are distributed and compare before enzymolysis more after enzymolysis solution
Sparse.
Target level of product quality
Organoleptic indicator
Physicochemical requirements
Moisture/(%)≤14%；Viscosity (6% 30 DEG C of starch gelatinization liquid) mPa S >=1800 (superfine, one-level, two grades),
>=800 (three-levels)；Ash/(%)≤0.4；Puerarin/(mg/kg) >=800 (superfine), >=400 (one-levels), >=100 (two grades),
>=25 (three-levels).
Laboratory sample brief summary
Experimental raw Amylum Puerariae Radicis are white, powdery, are visible by naked eyes impurity, and it is in homogeneous transparent colloidal substance afterwards that hot water rushes molten.Warp
Cross after α-amylase is processed instant kudzu vine powder is white or yellow-white, compare raw material Amylum Puerariae Radicis more soluble；Experiment records Amylum Puerariae Radicis
Moisture is 13.123% ＜ 14%, meets the physicochemical requirements of Amylum Puerariae Radicis product；This experiment records to extract in 50g Amylum Puerariae Radicis and obtains Huang
Ketone quality is 21.525mg, i.e. 430.5mg/kg ＞ 400mg/kg, meets the prescription of one-level Amylum Puerariae Radicis.
Summarize：
(1) adopt each factor of rheometer measurement to viscosity influence, find that large effect factor is：Solution concentration, shearing
Speed, heat time heating time, the impact to viscosity was less.
(2) found by experiment of single factor, the optimal conditionss of each factor are：Enzyme dosage is 0.8g, and heat time heating time is
20min, heating-up temperature is 60 DEG C, and drying time is 19h.
(3) adopt orthogonal experiment, determine that optimum process is combined as：Starch enzyme dosage is 0.8g, and reaction temperature is 60 DEG C,
Response time be 10min, drying time 19h.The product content of reducing sugar produced under this condition is high, and instant effect is preferable.
(4) this experiment is studied to the property indices of instant kudzu vine powder and antioxidant activity, wherein moisture, reducing sugar
As follows with total sugar, starch granuless structure and determination of total flavonoids result：13.123% is reached containing moisture in Amylum Puerariae Radicis；Instant Pueraria lobota
In powder, content of reducing sugar is 4.49%, and total sugar content is 39.1%, and wherein total sugar content is apparently higher than reducing sugar；With 70% second
Alcohol, as extractant, is extracted from 50g Amylum Puerariae Radicis using ultrasonic wave extraction and obtains crude flavonoid powder, and its extraction ratio is 2.38%；Real
The content of total flavones in Amylum Puerariae Radicis is determined in test, and result is to contain flavone quality in 50g Amylum Puerariae Radicis for 21.525mg；Amylum Puerariae Radicis before and after enzymolysis
The observation of kernel structure, before and after enzymolysis, starch particle shapes all assume irregular polygon, without the Amylum Puerariae Radicis particle surface light of enzymolysis
Cunning, flawless, distribution of particles are intensive, and the instant kudzu vine powder granule after digesting, surface shrinkage, little particle quantity reduces relatively,
Grain distribution is more loose.
Claims (2)
1. a kind of technique of Production by Enzymes distant Radix Puerariae powder is it is characterised in that comprise the steps：
(1) clean Radix Puerariae is cut into 2-3 centimetre of segment, adds water, pulverized pulping, the matter of Radix Puerariae and water with soy bean milk making machine
Amount ratio is 1：3-5, after pulverizing, carries out extruding and filters, filtrate is stood 48h with two-layer gauze, after its layering, siphon upper liquid
Body, and lower sediment is placed in 24h in 50 DEG C of baking ovens, until drying, the solid after drying is broken into pieces, continues in an oven to dry
24h, the solid pulverizer of moisture drying completely is ground into powder, that is, obtains powder of Radix Puerariae, be sealed against, in 4 DEG C of refrigerators
Stored refrigerated；
(2) configuration quality concentration is the powder of Radix Puerariae solution of 30-60%, adds amylase, and diastatic addition is Radix Puerariae grain weight
The 1-5% of amount, after gelatinizing 10-60min in 35 DEG C -65 DEG C of water bath with thermostatic control, being placed in 18h-45h in 50 DEG C of baking oven can make
Obtain distant Radix Puerariae powder.
2. the technique of the Production by Enzymes distant Radix Puerariae powder described in claim 1 is it is characterised in that in step (2), powder of Radix Puerariae solution
Mass concentration is 50%, added amylase, and diastatic addition is the 4% of powder of Radix Puerariae weight, in 50 DEG C of water bath with thermostatic control
After middle gelatinizing 20min, it is placed in 22h in 50 DEG C of baking oven and can be prepared by distant Radix Puerariae powder.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109090413A (en) * | 2018-07-20 | 2018-12-28 | 江南大学 | A kind of preparation method of mung bean pueraria lobata instant drink powder |
| CN114403343A (en) * | 2022-01-26 | 2022-04-29 | 广元市帆舟食品有限责任公司 | Pulping process and application thereof |
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| CN103141790A (en) * | 2013-01-26 | 2013-06-12 | 吉首大学 | Method for preparing instant Radix Puerariae powder through adopting compound enzyme |
| CN103416694A (en) * | 2013-09-03 | 2013-12-04 | 湖南农业大学 | Method for producing instant kudzu vine root powder through direct enzymolysis of kudzu vine root raw materials by adoption of compound enzymes |
| CN103739720A (en) * | 2013-12-06 | 2014-04-23 | 山东好当家海洋发展股份有限公司 | Method for preparing lobed kudzuvine root starch from lobed kudzuvine root |
| CN105454529A (en) * | 2015-12-21 | 2016-04-06 | 安徽山葛老天然食品有限公司 | Instant kudzu vine root milky tea |
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| CN103141790A (en) * | 2013-01-26 | 2013-06-12 | 吉首大学 | Method for preparing instant Radix Puerariae powder through adopting compound enzyme |
| CN103416694A (en) * | 2013-09-03 | 2013-12-04 | 湖南农业大学 | Method for producing instant kudzu vine root powder through direct enzymolysis of kudzu vine root raw materials by adoption of compound enzymes |
| CN103739720A (en) * | 2013-12-06 | 2014-04-23 | 山东好当家海洋发展股份有限公司 | Method for preparing lobed kudzuvine root starch from lobed kudzuvine root |
| CN105454529A (en) * | 2015-12-21 | 2016-04-06 | 安徽山葛老天然食品有限公司 | Instant kudzu vine root milky tea |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109090413A (en) * | 2018-07-20 | 2018-12-28 | 江南大学 | A kind of preparation method of mung bean pueraria lobata instant drink powder |
| CN114403343A (en) * | 2022-01-26 | 2022-04-29 | 广元市帆舟食品有限责任公司 | Pulping process and application thereof |
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