CN106417021A - Internet+ remote tissue culture management method - Google Patents
Internet+ remote tissue culture management method Download PDFInfo
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- CN106417021A CN106417021A CN201610873102.3A CN201610873102A CN106417021A CN 106417021 A CN106417021 A CN 106417021A CN 201610873102 A CN201610873102 A CN 201610873102A CN 106417021 A CN106417021 A CN 106417021A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06K—GRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
- G06K17/00—Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
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- Theoretical Computer Science (AREA)
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Abstract
The invention provides an Internet+ remote tissue culture management method, and belongs to a tissue culture management technology. An NFC chip and a recognizing device are added to a tool for tissue culture, in the whole tissue culture process including all stages from inoculation to strong seedling output of bottles and the like, the whole tissue culture process is recorded, tracked and adjusted by means of an NFC near-field communication technology and a computer system, a mobile terminal and a server are then connected through the Internet, and managers can remotely check and adjust the tissue process. The Internet+ remote tissue culture management method has the advantages of being modern, informationized and easy to manage.
Description
Technical field
The invention belongs to tissue culture's management technology, more particularly to a kind of the Internet+remote tissue training orientation method.
Background technology
Plant tissue culture（Broad sense）It is isolated culture again, refers to isolate the tissue, organ, thin for suiting the requirements from plant
Born of the same parents or protoplast etc., by sterile working, are cultivated to obtain whole plant or the life of regeneration under manual control condition
Produce the technology of other products with economic worth.Plant tissue culture（Narrow sense）Finger plant parts tissue, such as cambium layer,
Parenchyma, mesophyll tissue, endosperm etc. carry out culture and obtain regeneration plant, also refer to produce from each organ in incubation and heal
The culture of injured tissue, calluss are again through being differentiated to form aftergrowth again.By tissue culture technique, plant is multiplied
Rapid multiplying can be reached and the purpose of maternal plant merit is kept, while tissue culture technique is not limited by regions,
Through be widely used in famous and precious plant rapid multiplying, rareness species protection and make among famous and precious botanical derivative.
Whether produce or scientific research, particularly factorial praluction, tissue culture is belonging to extensive intensive work, with
When be required for per batch of tissue culture plant into excessively multiple propagation, change culture medium, different kinds needed using different trainings
Foster base, even if identical kind is required for using different culture medium under different phase or different culture purposes, wherein
Management process extremely complex and loaded down with trivial details, error is tended to by manual record tissue culture information, and traditional method many
Label paper, recording-related information is pasted in tissue culture body, but label paper is easily damaged in tissue culture's operation or dropped
And information loss is caused, managing to tissue culture increases difficulty, while the method using manually label paper is pasted on tissue culture bottle
The development trend of modern agriculture, information-based agriculture is not met.
NFC technology is recognized by non-contact radio-frequency（RFID）And Technology Integration differentiation of interconnecting,
Combining induction card reader, icah wavw and point-to-point function on one chip, can be in short distance and compatible equipment
It is identified data exchange.
Content of the invention
The problems referred to above are had based on prior art, the present invention provides a kind of the Internet+remote tissue training orientation method, its
By with the addition of NFC chip and identification equipment on the instrument of tissue culture, in tissue culture's overall process, including from being inoculated into
In each stage such as strong sprout bottle outlet, whole tissue culture is adjusted using NFC technology and computer system record, tracking
Process, in addition recycles the Internet connection mobile terminal server, so that management personnel is remotely checked and adjust tissue culture mistake
Journey, a kind of the Internet+remote tissue training orientation method that the present invention is provided have modernization, information-based, be easily managed excellent
Point.
A kind of the Internet+remote tissue training orientation method, which comprises the following steps：
Step S10 prepares culture medium, prepares culture medium, subpackage according to culture medium prescription and carries out high temperature sterilize, treats that culture medium is cold
But NFC chip base being loaded onto for tissue culture flasks after solidifying, recognizing device scan tissue culture bottle using NFC, computer is by tissue culture bottle
Match corresponding culture medium information；
Step S20 is inoculated with, advance typing tissue culture information, and tissue culture technology person scans oneself using the NFC device on aseptic operating platform
ID card, the plan according to computer arrangement is taken corresponding culture medium and scans record on NFC identification equipment, takes plant
Explant is inoculated with work accordingly, the tissue culture bottle after scanning inoculation is finished again after the completion of inoculation；
Manage between step S30 bottle, between bottle, management personnel check tissue culture plant daily, choose the tissue culture of contaminated or complete death
Bottle, scans record to contaminated and complete dead tissue culture bottle respectively on NFC identification equipment；
Step S40 is bred, and computer determines propagation number of times according to the growth coefficient of plant variety, and sends propagation information, tissue culture
Technician scans the ID card of oneself using the NFC device on aseptic operating platform, and being taken accordingly according to propagation information needs to increase
Batch is grown, taking out needs the tissue culture bottle of propagation to scan on NFC identification equipment and then propagation sterile working is carried out, after propagation
New bottle reuses NFC identification device scan, and the information in old bottle is added in new bottle information, and between execution step S30 bottle
Management, until it reaches default proliferation times；
Step S50 goes out root, and after default proliferation times are reached, computer sends out root information, and tissue culture technology person is using aseptic
NFC device on operating board scans the ID card of oneself, and being taken accordingly according to propagation information needs root batch, and taking out needs
The tissue culture bottle for going out root is scanned on NFC identification equipment and then carries out Chu Gen sterile working, and the new bottle for changing after bottle is reused NFC
Identification device scan, the information in old bottle is added in new bottle information, and is managed between execution step S30 bottle；
Step S60 strong sprout, after default proliferation times are reached, computer sends strong sprout information, and tissue culture technology person is using aseptic
NFC device on operating board scans the ID card of oneself, and being taken accordingly according to strong sprout information needs strong sprout batch, and taking out needs
The tissue culture bottle of strong sprout is scanned on NFC identification equipment and then carries out strong sprout sterile working, and the new bottle for changing after bottle is reused NFC
Identification device scan, the information in old bottle is added in new bottle information, and is managed between execution step S30 bottle；
NFC identification equipment is connected with computer, and the data for recording during tissue culture and calculate are uploaded onto the server by computer,
All tissue culture data that technical staff can be checked on server by mobile client；
In the overall process of tissue culture, management personnel can carry out long-range adjustment monitoring by mobile client to tissue culture process.
Wherein, execution step S11 also being needed before described step S10, culture medium prescription that advance typing will be prepared, joins
Personal information processed and automatically record preparation time.
Wherein, step S20 inoculation in tissue culture information include species numbering, goods number, production quantity, the production time and
Propagation number of times.
Wherein, also include step S21 in described step S20 inoculation, computer calculates each according to the information of record
The workload of tissue culture technology person.
Wherein, also include step S31 in managing between described step S30 bottle, computer is calculated according to the information of record
Kind pollution rate, mortality rate, technician's operational pollution rate.
Wherein, also include step S32 bottle outlet in managing between described step S30 bottle, the tissue cultured seedling after strong sprout reaches
Can with bottle outlet when, between bottle, management personnel take out and can record bottle outlet information using NFC identification device scan with the tissue cultured seedling of bottle outlet,
After scanned, Seedling is transferred between seedling exercising.
Wherein, the no root of quantification is inoculated with each tissue culture bottle in described step S40 propagation, and computer is according to group
Training bottle quantity converses plant generation number.
Wherein, which is made using the identification of NFC mechanics of communication, each bottle seedling of recording and tracking in whole tissue culture procedures
With the real-time record information of computer, calculate the work efficiency of tissue culture technology person, pollution rate, volume of production and to manage control whole
Individual tissue culture procedures.
Beneficial effects of the present invention：Recognize and track each bottle of tissue cultured seedling by NFC technology, recycle and calculate
The information that collects is counted by machine software, is calculated and data required for production or scientific research, such as pollution rate, teething rate and
Value-added coefficient, production efficiency, production quantity etc..Avoid because tissue cultured seedling quantity is too many and cause artificial missing error.
Specific embodiment
With reference to specific embodiment, the invention will be further described.
A kind of the Internet+remote tissue training orientation method, it is characterised in which comprises the following steps：
Step S10 prepares culture medium, when culture medium prescription, formulator's information and the automatic record that typing will be prepared is prepared
Between, culture medium, subpackage are prepared according to culture medium prescription and high temperature sterilize is carried out, be tissue culture flasks after culture medium cooled and solidified
NFC chip base being loaded onto, device scan tissue culture bottle being recognized using NFC, tissue culture bottle is matched corresponding culture medium information by computer；
Step S20 is inoculated with, and advance typing species numbering, goods number, production quantity, production time and propagation number of times etc. tissue culture are believed
Breath, tissue culture technology person scans the ID card of oneself using the NFC device on aseptic operating platform, and the plan according to computer arrangement takes
Record being scanned with corresponding culture medium on NFC identification equipment, takes plant explant and be inoculated with work accordingly, be inoculated with
Tissue culture bottle after scanning inoculation is finished again after becoming, computer calculates the work of each tissue culture technology person according to the information of record
Amount.
Manage between step S30 bottle, between bottle, management personnel check tissue culture plant daily, choose contaminated or complete death
Tissue culture bottle, scans record to contaminated and complete dead tissue culture bottle respectively on NFC identification equipment, and computer is according to record
Information calculates kind pollution rate, mortality rate, technician's operational pollution rate, and the tissue cultured seedling after strong sprout reaches can be with bottle outlet
When, between bottle, management personnel take out and can record bottle outlet information with the tissue cultured seedling of bottle outlet using NFC identification device scan, scanned
Afterwards Seedling is transferred between seedling exercising.
Step S40 is bred, and computer determines propagation number of times according to the growth coefficient of plant variety, and sends propagation information,
Tissue culture technology person scans the ID card of oneself using the NFC device on aseptic operating platform, takes corresponding need according to propagation information
Batch to be bred, taking out needs the tissue culture bottle of propagation to scan on NFC identification equipment and then carry out propagation sterile working, each group
The no root of quantification is inoculated with training bottle, and computer converses plant generation number according to tissue culture bottle quantity, after propagation
New bottle reuses NFC identification device scan, and the information in old bottle is added in new bottle information, and between execution step S30 bottle
Management, until it reaches default proliferation times.
Step S50 goes out root, and after default proliferation times are reached, computer sends out root information, and tissue culture technology person uses
NFC device on aseptic operating platform scans the ID card of oneself, and being taken accordingly according to propagation information needs root batch, takes out
Need the tissue culture bottle of root to scan on NFC identification equipment and then Chu Gen sterile working is carried out, the new bottle for changing after bottle is made again
Device scan is recognized with NFC, the information in old bottle is added in new bottle information, and manage between execution step S30 bottle.
Step S60 strong sprout, after default proliferation times are reached, computer sends strong sprout information, and tissue culture technology person uses
NFC device on aseptic operating platform scans the ID card of oneself, and being taken accordingly according to strong sprout information needs strong sprout batch, takes out
Need the tissue culture bottle of strong sprout to scan on NFC identification equipment and then strong sprout sterile working is carried out, the new bottle for changing after bottle is made again
Device scan is recognized with NFC, the information in old bottle is added in new bottle information, and manage between execution step S30 bottle.
Using the identification of NFC mechanics of communication, each bottle seedling of recording and tracking in whole tissue culture procedures, using computer
Real-time record information, calculates the work efficiency of tissue culture technology person, pollution rate, volume of production and manages the whole tissue training of control
Foster flow process.
NFC identification equipment is connected with computer, and the data for recording during tissue culture and calculate are uploaded to clothes by computer
Business device, all tissue culture data that technical staff can be checked on server by mobile client.
In the overall process of tissue culture, management personnel can carry out long-range adjustment prison by mobile client to tissue culture process
Control.
Embodiment described above only have expressed one embodiment of the present invention, and its description is more concrete and detailed, but simultaneously
The restriction that therefore can not be interpreted as to the scope of the claims of the present invention.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, some deformation can also be made and improved, these belong to the guarantor of the present invention
Shield scope.Therefore, the protection domain of patent of the present invention should be defined by claims.
Claims (8)
1. a kind of the Internet+remote tissue training orientation method, it is characterised in which comprises the following steps：
Step S10 prepares culture medium, prepares culture medium, subpackage according to culture medium prescription and carries out high temperature sterilize, treats that culture medium is cold
But NFC chip base being loaded onto for tissue culture flasks after solidifying, recognizing device scan tissue culture bottle using NFC, computer is by tissue culture bottle
Match corresponding culture medium information；
Step S20 is inoculated with, advance typing tissue culture information, and tissue culture technology person scans oneself using the NFC device on aseptic operating platform
ID card, the plan according to computer arrangement is taken corresponding culture medium and scans record on NFC identification equipment, takes plant
Explant is inoculated with work accordingly, the tissue culture bottle after scanning inoculation is finished again after the completion of inoculation；
Manage between step S30 bottle, between bottle, management personnel check tissue culture plant daily, choose the tissue culture of contaminated or complete death
Bottle, scans record to contaminated and complete dead tissue culture bottle respectively on NFC identification equipment；
Step S40 is bred, and computer determines propagation number of times according to the growth coefficient of plant variety, and sends propagation information, tissue culture
Technician scans the ID card of oneself using the NFC device on aseptic operating platform, and being taken accordingly according to propagation information needs to increase
Batch is grown, taking out needs the tissue culture bottle of propagation to scan on NFC identification equipment and then propagation sterile working is carried out, after propagation
New bottle reuses NFC identification device scan, and the information in old bottle is added in new bottle information, and between execution step S30 bottle
Management, until it reaches default proliferation times；
Step S50 goes out root, and after default proliferation times are reached, computer sends out root information, and tissue culture technology person is using aseptic
NFC device on operating board scans the ID card of oneself, and being taken accordingly according to propagation information needs root batch, and taking out needs
The tissue culture bottle for going out root is scanned on NFC identification equipment and then carries out Chu Gen sterile working, and the new bottle for changing after bottle is reused NFC
Identification device scan, the information in old bottle is added in new bottle information, and is managed between execution step S30 bottle；
Step S60 strong sprout, after default proliferation times are reached, computer sends strong sprout information, and tissue culture technology person is using aseptic
NFC device on operating board scans the ID card of oneself, and being taken accordingly according to strong sprout information needs strong sprout batch, and taking out needs
The tissue culture bottle of strong sprout is scanned on NFC identification equipment and then carries out strong sprout sterile working, and the new bottle for changing after bottle is reused NFC
Identification device scan, the information in old bottle is added in new bottle information, and is managed between execution step S30 bottle；
NFC identification equipment is connected with computer, and the data for recording during tissue culture and calculate are uploaded onto the server by computer,
All tissue culture data that technical staff can be checked on server by mobile client；
In the overall process of tissue culture, management personnel can carry out long-range adjustment monitoring by mobile client to tissue culture process.
2. a kind of the Internet according to claim 1+remote tissue training orientation method, it is characterised in that step S10 it
Front also need execution step S11, culture medium prescription that advance typing will be prepared, formulator's information and when record is prepared automatically
Between.
3. a kind of the Internet according to claim 1+remote tissue training orientation method, it is characterised in that step S20 connects
Tissue culture information in kind includes species numbering, goods number, production quantity, production time and propagation number of times.
4. a kind of the Internet according to claim 1+remote tissue training orientation method, it is characterised in that step S20 connects
Also include step S21 in kind, computer calculates the workload of each tissue culture technology person according to the information of record.
5. a kind of the Internet according to claim 1+remote tissue training orientation method, it is characterised in that step S30 bottle
Between manage in also include step S31, computer according to record information calculate kind pollution rate, mortality rate, technician operation
Pollution rate.
6. a kind of the Internet according to claim 1+remote tissue training orientation method, it is characterised in that step S30 bottle
Between manage in also include step S32 bottle outlet, the tissue cultured seedling after strong sprout reach can with bottle outlet when, between bottle management personnel take out can
With the tissue cultured seedling of bottle outlet, record bottle outlet information using NFC identification device scan, scanned after Seedling is transferred between seedling exercising.
7. a kind of the Internet according to claim 1+remote tissue training orientation method, it is characterised in that step S40 increases
Grow the no root that quantification is inoculated with each tissue culture bottle, computer converses plant generation number according to tissue culture bottle quantity.
8. a kind of the Internet according to claim 1+remote tissue training orientation method, it is characterised in which is whole
Using the identification of NFC mechanics of communication, each bottle seedling of recording and tracking in tissue culture procedures, letter is recorded real-time using computer
Breath, calculates the work efficiency of tissue culture technology person, pollution rate, volume of production and manages the whole tissue culture procedures of control.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610873102.3A CN106417021A (en) | 2016-10-08 | 2016-10-08 | Internet+ remote tissue culture management method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610873102.3A CN106417021A (en) | 2016-10-08 | 2016-10-08 | Internet+ remote tissue culture management method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106873459A (en) * | 2017-02-23 | 2017-06-20 | 佛山市融信通企业咨询服务有限公司 | A kind of tissue culture culture medium Nutrition monitoring system |
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2016
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| CN101826179A (en) * | 2010-04-16 | 2010-09-08 | 陶然 | Orchild intelligent management system |
| CN103782954A (en) * | 2014-01-25 | 2014-05-14 | 广东燕塘乳业股份有限公司 | Cow fine breeding method based on RFID technology |
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Application publication date: 20170222 |
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