CN106396836A - A biological seed coating agent for controlling soybean phytophthora root rot, a preparing method thereof and a using method of the seed coating agent - Google Patents
A biological seed coating agent for controlling soybean phytophthora root rot, a preparing method thereof and a using method of the seed coating agent Download PDFInfo
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- CN106396836A CN106396836A CN201610605504.5A CN201610605504A CN106396836A CN 106396836 A CN106396836 A CN 106396836A CN 201610605504 A CN201610605504 A CN 201610605504A CN 106396836 A CN106396836 A CN 106396836A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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Abstract
The invention provides a biological seed coating agent for controlling soybean phytophthora root rot, a preparing method thereof and a using method of the seed coating agent and belongs to the technical field of agricultural biotechnology. The seed coating agent comprises 20% by mass of streptomyces dorenlacusensis M1-4 inoculum, 10% by mass of a plant activator, 5% by mass of a biological conditioning agent, 10% by mass of a nutrition element solution and 10% by mass of an auxiliary agent, with the balance being water. The preparing method includes adding 1% of carboxymethylcellulose sodium and 10% of polyvinyl alcohol into water, heating to 80 DEG C, stirring for 20 min until film forming agents are fully dissolved, cooling to room temperature, adding 0.4% of rhodamine, 3% of glycerol and 0.5% of peregal O-25, and adding 20% of the actinomycete streptomyces M1-4 inoculum, 5% of the biological conditioning agent, 10% of the nutrition element solution, 10% of the plant activator and the water into the auxiliary agent according to mass percentages of components in the seed coating agent. The seed coating agent significantly inhibits soybean diseases and pests, can increase the root shoot ratio, can increase soybean stress resistance and can increase soybean quality and yields.
Description
Technical field
The invention belongs to agricultural biological technical field and in particular to a kind of soybean phytophthora root rot preventing and treating biological seed coating agent and
Its preparation method, using method.
Background technology
Seed coating technology is initially the thirties in 20th century, the success first on crop of Germain seeds company of Britain
Develop coating agent for seed, the Soviet Union developed and compounded by polymeric set compound adhesive agent and plant growth regulator the initial stage eighties
Seed coat agent, seed coat agent technology develops by leaps and bounds.At present, the seed coating technology of agriculture developed country and degree be all in the world
Very high, contain Semen Maydiss, Oryza sativa L., Semen Tritici aestivi, Semen sojae atricolor, millet, vegetable, Cotton Gossypii, Semen arachidis hypogaeae, vegetable etc..
The research of China's seed coat agent and application, start to develop very fast from the eighties in 20th century, up to the present in crops
In commonly use, in China market, existing popular main seed coat agent is chemical seed coat agent, its main component around phenylate, cough up bacterium
The chemical drugss such as nitrile, Fluoxastrobin, Diacloden, metalaxyl, Tebuconazole, imidacloprid, triticonazole, Silthiopham are prepared from.Make
With during there is certain risk and harm.The general composition of these chemical seed coat agent products is single or function is unilateral or poisonous
Property high, there is certain risk and harm in use.Although occurring in that a small amount of biological seed coating agent in the market, solution
Determine seed coat agent toxic residue problem, but action effect is not notable in terms of diseases prevention insect protected.
Content of the invention
In order to overcome the shortcomings of background technology presence, the present invention provides a kind of soybean phytophthora root rot to prevent and treat biological seed coating agent
And preparation method thereof, using method, this seed coat agent have environmental protection, height suppress soybean phytophthora root rot generation, improve crop
Anti-adversity ability, the comprehensive function of strong sprout, raising yield and improving quality.
This soybean phytophthora root rot preventing and treating biological seed coating agent is made up of following component by mass percentage：20% Duo Lunhu
Streptomycete (Streptomyces dorenlacusensis) M1-4 microbial inoculum, 10% activating plants agent, 5% biological regulation
Agent, 10% nutrient solution, 10% auxiliary agent, balance of water；Described activating plants agent is concentration is 0.5mmol/L benzene
And thiadiazoles aqueous solution；Described biological regulator is the uniconazole P aqueous solution of concentration 2mg/L.
It is micro- that described Duolun lake streptomycete (Streptomyces dorenlacusensis) M1-4 microbial inoculum is preserved in China
Biological inoculum preservation administration committee common micro-organisms center, preserving number is CGMCC No 11789；Preservation address, court of Beijing
Positive area North Star West Road 1 institute 3, preservation date on December 4th, 2015.Duolun lake streptomycete M1-4 microbial inoculum is to causing soybean phytophthora
The main pathogenic fungi of root rot, has relatively including soybean phytophthora (P.sojae), Fusarium oxysporum (Fusariumoxysporun)
High inhibition effect, in addition equally inhibited to other pathogen, such as Herba bromi japonici Fusariumsp (Fusarium
Avenaceum), gibberella saubinetii (FusariumGraminearu), Fusarium graminearum (Fusarium graminearum),
Microbial inoculum spore concentration is >=1011Individual/ml.
Described activating plants agent is concentration is 0.5mmol/L diazosulfide aqueous solution, by 0.5mmol diazosulfide
With after the dissolving of 10ml ethanol plus distilled water is settled to 1L and makes.Do not have bactericidal action in vitro diazosulfide, right
Described streptomycete does not have inhibitory action.This activating plants agent is used for protecting agricultural plant disease and the preventing and treating of gardening plant disease,
Mainly pass through to induce crop to produce the resistance to pest and disease damage for the disease resistance substance activating plant, the silk floss that controlling object includes Oomycete is mould
Genus, Aphanomyces, pythium, Phytophthora, Sclerospora, Plasmopara, Pseudoperonospora, Peronospora etc. more than 20 belongs to generation
Disease.The disease resistance that induction produces has long-acting and wide spectrum characteristic, and the disease resisting effect that induction produces has hysteresis quality, is difficult to produce
Raw Drug resistance, no affects on the mushroom not having pathogenic, is conducive to maintaining ecological balance and carries out environmental conservation.
Described nutrient solution is made up of following component by mass percentage：0.5% ammonium molybdate, 1% boric acid,
1% zinc sulfate, 1% manganese sulfate, 1% NaFeEDTA, balance of water.It is quick that component in this nutrient solution is Semen sojae atricolor
Inductive element, appropriate interpolation all has good result to the yield and quality of Semen sojae atricolor.
Described biological regulator is the uniconazole P aqueous solution of concentration 2mg/L, and 2mg uniconazole P is dissolved in 10ml ethanol, uses
Distilled water is settled to 1L and makes.Uniconazole P can adjust plant root/shoot ratio, adjusts gibberellins, abscisic acid and cell division in plant
The endogenous hormones such as element, improve the opposing to adverse circumstance for the plant.
Described auxiliary agent is made up of following component by mass percentage：1% sodium carboxymethyl cellulose, 10% polyethylene
Alcohol, 0.4% rhodamine B, 3% glycerol, 0.5% peregal O-25, balance of water.This auxiliary agent has slow-release function,
The action period of seed coat agent can be extended, its film property and coating strength reach like product standard.Described auxiliary agent is to funguses, unwrapping wire
Bacterium and nanobacteria innocuous or hypotoxicity, streptomycete M1-4 can be survived in seed coat agent, can breed in soil, strengthens suppression
Soyabean phytophthora acts on.
The preparation method that this soybean phytophthora root rot prevents and treats biological seed coating agent is as follows：Percent mass by component each in auxiliary agent
It is added to the water than by the polyvinyl alcohol of 1% sodium carboxymethyl cellulose and 10%, be heated to 80 DEG C, stir 20 minutes to film former
It is completely dissolved, after dropping to room temperature, the rhodamine B of addition 0.4%, 3% glycerol, 0.5% peregal O-25 make auxiliary agent；
The mass percent pressing each component in seed coat agent again adds 20% Duolun lake streptomycete M1-4,5% biological modulated in auxiliary agent
Section agent, 10% nutrient solution, 10% activating plants agent and the water of surplus, are prepared into multiple pathogenic bacterias and insect pest to Semen sojae atricolor
Inhibited seed coat agent.
This is by seed coat agent and soybean seed according to mass ratio 1：(20-30) ratio mixing, air-dries under cool environment, obtains
Semen sojae atricolor capsuled seed.
The present invention has the advantages that：This soybean phytophthora root rot preventing and treating biological seed coating agent is used for preventing and treating soybean seedling
Root disease, especially has good preventive and therapeutic effect to soybean phytophthora root rot；Improve Semen sojae atricolor resistance, to environment-stress and acyclic
The resistance of border stress all has enhancing；Increase root/shoot ratio, play the effect of strong sprout；Supplement Semen sojae atricolor sensitive elements, promote soybean yields
Formed, improve soybean quality.This seed coat agent is suspension type biological seed coat agent, and storage has and rocks under layering, room temperature and can recover
Original state.Product quality meets national standard seed coat agent code requirement：PH value is 6.5-7.5, suspensibility >=98%, viscosity≤
0.06Pa s (25 DEG C), expulsion rate≤5%, film property and low temperature, hot storage stability are qualified.
Brief description
Fig. 1 is the soybean phytophthora potted plant figure of morbidity.
Fig. 2 is soybean biological seed coating agent to soybean phytophthora potted plant preventive effect figure.
Specific embodiment
With reference to Fig. 1 and Fig. 2, the present invention will be further described：
This soybean phytophthora root rot preventing and treating biological seed coating agent is made up of following component by mass percentage：20% Duolun lake strepto-
Bacterium (Streptomyces dorenlacusensis) M1-4 microbial inoculum, 10% activating plants agent, 5% biological regulator,
10% nutrient solution, 10% auxiliary agent, balance of water；The spore concentration of described Duolun lake streptomycete M1-4 microbial inoculum is
≥1011Individual/ml；Described activating plants agent is concentration is 0.5mmol/L diazosulfide aqueous solution；Described biological regulator
Uniconazole P aqueous solution for concentration 2mg/L.
Soybean phytophthora root rot preventing and treating biological seed coating agent in described embodiment is prepared by following methods：(1) preparation helps
Agent：By the mass percent of component each in auxiliary agent, the polyvinyl alcohol of 1% sodium carboxymethyl cellulose and 10% is added to the water, plus
Heat to 80 DEG C, stirring are completely dissolved for 20 minutes to film former, drop to add after room temperature 0.4% rhodamine B, 3% glycerol,
0.5% peregal O-25 makes auxiliary agent.(2) prepare activating plants agent：0.5mmol diazosulfide is molten with 10ml ethanol
After solution plus distilled water is settled to 1L and makes.(3) prepare biological regulator：2mg uniconazole P is dissolved in 10ml ethanol, uses distilled water
It is settled to 1L to make.(4) prepare nutrient solution：By mass percentage by 0.5% ammonium molybdate, 1% boric acid, 1%
Zinc sulfate, 1% manganese sulfate, 1% NaFeEDTA are dissolved in the water of surplus and making.(5) prepare seed coat agent：By each in seed coat agent
The mass percent of component adds 20% Duolun lake streptomycete (Streptomyces dorenlacusensis) in auxiliary agent
M1-4 microbial inoculum, 5% biological regulator, 10% nutrient solution, 10% activating plants agent and the water of surplus are made.
Wherein, peregal O-25, rhodamine B are purchased in Tianjin auspicious sign Dye Co., Ltd；Ammonium molybdate, boric acid, sulphuric acid
Zinc, manganese sulfate, glycerol are purchased in Tianjin great Mao chemical reagent factory；Uniconazole P, NaFeEDTA, sodium carboxymethyl cellulose and poly- second
Enol purchases Tianjin Yong great chemical reagent company limited；Diazosulfide (BTH) is purchased limited in the clear biotechnology that continues in Shanghai
Company.
The screening technique of described Duolun lake streptomycete (Streptomyces dorenlacusensis) M1-4, including such as
Lower step：
(1) gather bed mud sample from lake, dry in dark ventilation, become powder with mortar grinder；
(2) carry out separating using dilution method, soil sample is put in the bottle equipped with bead and sterilized water, concussion, after standing, dilute
Release, preparation mass concentration is 10-3、10-4、10-5Diluent, draws diluent and is added drop-wise to addition cycloheximide (50 μ g/ml) and naphthalene
On the Gause I culture medium flat plate of pyridine keto acid (40 μ g/ml), coating uniformly, culture dish is placed on incubator to be inverted culture, growth
28 DEG C of temperature, cultivates 5-7 days, isolates and purifies；
(3) there are the actinomycetes of inhibitory action to soyabean phytophthora using flat board face-off method screening：By the actinomycetes strain of purification, use
Gause I culture medium culturing, actinomycetes are transferred to Radix Dauci Sativae culture medium center, are cultivated 3 days with Φ 9cm culture dish；Greatly
After bean phytophthora is cultivated 4-5 days in Radix Dauci Sativae culture medium, it is cut into Φ 0.5cm mycelia block, is inoculated into the training containing Radix Dauci Sativae culture medium
Foster ware surrounding decussation line position, culture observed measurement antibacterial circle diameter after 7-10 days, selected the obvious unwrapping wire of antagonism
Bacterium, preserves.This bacterial strain thus it is speculated that this bacterium is streptomyces novel species, and will be named as many after physiological and biochemical property and Molecular Identification
Human relations lake streptomycete (Streptomyces dorenlacusensis) M1-4.
The preparation method of described Duolun lake streptomycete (Streptomyces dorenlacusensis) M1-4 microbial inoculum：
(1) amplification culture of strain：Take the conidium of Duolun lake streptomycete M1-4 bacterial strain, be inoculated into liquid seed culture medium
In, shaking table culture obtains actinomycetes liquid seeds；28 DEG C of shaking table culture temperature, rotating speed 180r/min cultivates 48h.Described liquid strain
The formula of sub- culture medium is：Yeast extract 4g/L, glucose 4g/L, Fructus Hordei Germinatus extract 10g/L and CaCO32g/L, pH
7.3；
(2) preparation of microbial inoculum：Soybean cake powder and Testa Tritici are pressed in mass ratio 1:Moisture content is adjusted to 55%, pH after 1 mix homogeneously
To 8, in every Kg substrate, add 5g CaCO3, after mixing, after loading 121 DEG C of sterilizing 30min in fermentation flask, in mass ratio 15%
Ratio accesses step (1) bacterium solution prepared, 28-30 DEG C, mixing speed be 60rpm/min, fermentation culture 7 days, add sterilized water
Filter, prepared Duolun lake streptomycete M1-4 microbial inoculum, the spore concentration of Duolun lake streptomycete M1-4 microbial inoculum is >=1011Individual/ml.
This soybean phytophthora root rot prevents and treats the potted plant efficiency test to soybean phytophthora root rot prevention effect for the biological seed coating agent：
With soybean phytophthora root rot susceptible variety pacify agriculture 28 as material, with fall ill and soybean biomass index of correlation investigate be according to
According to.Potted plant use 15cm flowerpot, every basin loads 500g continuous cropping soil.Seed coat agent and soybean seed are according to mass ratio 1：30 mixing,
Air-dry under cool environment, obtain Semen sojae atricolor capsuled seed.The seed that processed seed coat agent and untreated seed are seeded in respectively
In basin, 10 seeds sowed by every basin, keep a full stand of seedings 6 plants, are repeated 4 times.Grow to 35 days sampling statistics occurring degrees in basin, calculate disease
Feelings index, measures overground part fresh weight, and root fresh weight investigates growth-promoting effect, is shown in Table 1.Soybean biological seed coating agent is processed, and shows preferably
Prevention effect, its sickness rate prevention effect more than 70%；Soybean biological seed coating agent has obvious growth-promoting functions, can promote big
Beanstalk leaf fresh weight, the increase of root fresh weight, increase by 87% and 125% respectively.
The each impact processing to soybean phytophthora root rot prevention effect, Semen sojae atricolor growth of table 1 greenhouse pot culture experiment
Note：Soybean phytophthora root rot occurring degree is divided into 6 grades, does not fall ill for root system for 0 grade；1 grade is root system sickness rate≤30%, leaf
Piece is normal；2 grades is 30%<Root system sickness rate≤60%, blade is normal；3 grades is 60%<Root system sickness rate≤80%；Blade becomes
Huang, 4 grades is root system sickness rate more than 80%, and blade is withered, and 5 grades is that whole strain is dead, and blade dries up.
Disease index and prevention effect calculate respectively according to the following formula：
Disease index=[(sick level strain number x representative value)/(total strain number x highest disease level representative value)] x100,
Prevention effect=[(comparison disease index-process disease index)/comparison disease index] × 100%.
This soybean phytophthora root rot prevents and treats the land for growing field crops efficiency test to soybean phytophthora root rot prevention effect for the biological seed coating agent：
Experiment is in Heilongjiang Province Daqing Lindian County Heilongjiang Bayi Agricultural Reclamation University Experimental Base and Heilongjiang Province's Bao Quan ridge scientific research
Second crop soil of standing is carried out.To the east of agriculture 42 and Sui Nong 28 be material, seed coat agent and soybean seed are according to mass ratio 1：30 mixing, shady and cool ring
Air-dry under border, obtain Semen sojae atricolor capsuled seed.Investigated as foundation with the morbidity of different regions different cultivars and yield index of correlation, biological
Seed coat agent has good preventive and therapeutic effect to phytophthora root rot of soybean.Application soybean phytophthora root rot preventing and treating biological seed coating agent kind makes Semen sojae atricolor
Root rot sickness rate is reduced to 7.55～12.31 by 24.25～37.66, and death rate is reduced to 1.25 by 6.25～10.91～
6.67, it is shown in Table 2.Apply this biological seed coating agent to increased the survival rate of seedlings of Semen sojae atricolor, single-strain grain number, 100-grain weight, yield increase by 24% with
On.
The impact to soybean phytophthora root rot prevention effect and soybean yields for table 2 seed coat agent
Note：Number application SPSS software analysis, there is significant difference in 0.05 level in each column difference lowercase letter.
Claims (6)
1. a kind of soybean phytophthora root rot prevents and treats biological seed coating agent, and this seed coat agent is made up of following component by mass percentage：
20% Duolun lake streptomycete (Streptomyces dorenlacusensis) M1-4 microbial inoculum, 10% activating plants agent, 5%
Biological regulator, 10% nutrient solution, 10% auxiliary agent, balance of water；Described activating plants agent is that concentration is
0.5mmol/L diazosulfide aqueous solution；Described biological regulator is the uniconazole P aqueous solution of concentration 2mg/L.
2. a kind of soybean phytophthora root rot preventing and treating biological seed coating agent according to claim 1 it is characterised in that：Described battalion
Foster Element Solution is made up of following component by mass percentage：0.5% ammonium molybdate, 1% boric acid, 1% zinc sulfate, 1%
Manganese sulfate, 1% NaFeEDTA, balance of water.
3. a kind of soybean phytophthora root rot preventing and treating biological seed coating agent according to claim 1 it is characterised in that：Described helps
Agent is made up of following component by mass percentage：1% sodium carboxymethyl cellulose, 10% polyvinyl alcohol, 0.4% rhodamine
B, 3% glycerol, 0.5% peregal O-25, balance of water.
4. a kind of soybean phytophthora root rot preventing and treating biological seed coating agent according to claim 1 it is characterised in that：Described is many
The spore concentration of human relations lake streptomycete M1-4 microbial inoculum is >=1011Individual/ml.
5. soybean phytophthora root rot described in claim 1 prevent and treat biological seed coating agent preparation method it is characterised in that：By auxiliary agent
In the mass percent of each component the polyvinyl alcohol of 1% sodium carboxymethyl cellulose and 10% is added to the water, be heated to 80 DEG C,
Stirring is completely dissolved for 20 minutes to film former, drop to add after room temperature 0.4% rhodamine B, 3% glycerol, 0.5% flat
Flat plus O-25 makes auxiliary agent；The mass percent pressing each component in seed coat agent again adds 20% Duolun lake streptomycete in auxiliary agent
M1-4 microbial inoculum, 5% biological regulator, 10% nutrient solution, 10% activating plants agent and the water of surplus, are prepared into
Multiple pathogenic bacterias and the inhibited seed coat agent of insect pest to Semen sojae atricolor.
6. soybean phytophthora root rot described in claim 1 prevent and treat biological seed coating agent using method it is characterised in that：Clothing will be planted
Agent and soybean seed are according to mass ratio 1：(20-30) ratio mixing, air-dries under cool environment, obtains Semen sojae atricolor capsuled seed.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108587981A (en) * | 2018-05-30 | 2018-09-28 | 西南大学 | Multi-functional molten algae streptomycete Streptomyces amritsarensis and its application |
| CN111333454A (en) * | 2020-04-01 | 2020-06-26 | 黑龙江八一农垦大学 | Disease-preventing growth-promoting biological small red bean seed coating agent and preparation method and use method thereof |
| CN111393227A (en) * | 2020-04-08 | 2020-07-10 | 黑龙江八一农垦大学 | Seed coating agent |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102433281A (en) * | 2011-12-16 | 2012-05-02 | 华南农业大学 | Streptomyces katrae NB20, as well as culture method and application thereof |
| CN103131646A (en) * | 2011-11-29 | 2013-06-05 | 中国科学院沈阳应用生态研究所 | Germ bacteria agent and preparation method and application thereof |
| CN103992967A (en) * | 2014-04-16 | 2014-08-20 | 中国中化股份有限公司 | Streptomyces albidoflavus and its application in plant disease control |
| CN105385623A (en) * | 2015-11-20 | 2016-03-09 | 江苏省农业科学院 | Actinomycete strain JSX-1 for preventing and curing phytophthora sojae and fungicide thereof |
-
2016
- 2016-07-28 CN CN201610605504.5A patent/CN106396836B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103131646A (en) * | 2011-11-29 | 2013-06-05 | 中国科学院沈阳应用生态研究所 | Germ bacteria agent and preparation method and application thereof |
| CN102433281A (en) * | 2011-12-16 | 2012-05-02 | 华南农业大学 | Streptomyces katrae NB20, as well as culture method and application thereof |
| CN103992967A (en) * | 2014-04-16 | 2014-08-20 | 中国中化股份有限公司 | Streptomyces albidoflavus and its application in plant disease control |
| CN105385623A (en) * | 2015-11-20 | 2016-03-09 | 江苏省农业科学院 | Actinomycete strain JSX-1 for preventing and curing phytophthora sojae and fungicide thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108587981A (en) * | 2018-05-30 | 2018-09-28 | 西南大学 | Multi-functional molten algae streptomycete Streptomyces amritsarensis and its application |
| CN108587981B (en) * | 2018-05-30 | 2021-10-08 | 西南大学 | Streptomyces amyrosarensis and application thereof |
| CN111333454A (en) * | 2020-04-01 | 2020-06-26 | 黑龙江八一农垦大学 | Disease-preventing growth-promoting biological small red bean seed coating agent and preparation method and use method thereof |
| CN111393227A (en) * | 2020-04-08 | 2020-07-10 | 黑龙江八一农垦大学 | Seed coating agent |
| CN111393227B (en) * | 2020-04-08 | 2022-07-08 | 黑龙江八一农垦大学 | Seed coating agent |
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