CN106390197B - One kind stress active mass's engineering scaffold material and preparation method thereof - Google Patents
One kind stress active mass's engineering scaffold material and preparation method thereof Download PDFInfo
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- CN106390197B CN106390197B CN201610945937.5A CN201610945937A CN106390197B CN 106390197 B CN106390197 B CN 106390197B CN 201610945937 A CN201610945937 A CN 201610945937A CN 106390197 B CN106390197 B CN 106390197B
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- polyvinyl alcohol
- fibroin albumen
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/26—Mixtures of macromolecular compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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Abstract
The present invention relates to one kind by polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition stress active mass's engineering scaffold material and preparation method thereof, belong to biomedical materials field, described stress fibroin albumen and polyvinyl alcohol mass ratio be 1/9~1/1 in active mass's engineering scaffold material, the mass ratio of Kynoar and polyvinyl alcohol is 1/99~5/95, preparation method uses the suspension of polyvinylidene fluoride nanometer fiber, silk fibroin protein solution or fibroin albumen powder dispersion and poly-vinyl alcohol solution mix to obtain uniform and stable mixing suspension, it is formed through casting die, cryocoagulation, demoulding, freeze-drying obtains cavernous body, it handles and is prepared using vapour phase esterification, the tissue engineering bracket material has uniform and stable micro-structure, good biocompatibility, provide biological tissue The matrix condition of growth is assigned to material by the piezoelectricity response characteristic after external force.
Description
Technical field
The present invention relates to one kind stress active mass's engineering scaffold material and preparation method thereof, belong to bio-medical material neck
Domain.
Background technique
Organizational project is the emerging interdisciplinary field to grow up in the late three decades, wherein most basic thinking is in body
In outer separation, culture cell inoculation to the bracket with certain space structure, by mutually sticking between cell, increasing is grown
It grows, extracellular matrix secretion, to form the tissue and organ with certain structure and function, the bracket of this certain space structure
It is referred to as tissue engineering bracket, such as skin, bone, cartilage, tendon, ligament.Need to meet four items as tissue engineering bracket
Part is suitble to biocompatible materials, the pore structure for providing cell growth and the cambium of cell tactophily to be adapted
Degradation characteristic, the structure and mechanical property for meeting tissue engineering bracket.From timbering material in tissue repair or the process mesh of reconstruction
Predecessor is confined to occupancy substitution and repairs or based on occupancy rebuilds, for tissue growth provide physical space and matrix environment,
Respective reaction cannot be made with physiological stress, electromagnetic action or biochemical stimulate as living tissue, i.e., do not had biological anti-
Ying Xing, and biological respinse have to the foundation of the neoblastic speed of growth, bioactivity and with the healing of matrix etc. it is important
Meaning.Therefore, exploitation has the response of bionical physiological stress, the biological reactivity material of galvanomagnetic-effect becomes current tissue and repairs
The Main Trends of The Development of multiple material.
Many tissues are natural piezoelectrics in human body, such as: skin, bone, cartilage, tendon, ligament, piezoelectric effect are derived from
Collagen component in tissue can maintain the normal physiological activity of tissue and new by completing the mutual conversion of mechanical energy and electric energy
Old metabolism, the moulding, reconstruction and function for being conducive to tissue maintain, and tissue can promote tissue because of the polarization charge that piezoelectric effect generates
Growth and healing.This piezoelectric effect be exactly for biology angle biology stress activity.Due to the piezoelectricity of tissue
I.e. stress key effect of the activity characteristic in tissue reconstruction and repair process, will be tissue engineering bracket material design
A kind of new thinking is provided with building.
Kynoar is one of the high molecular material with best piezoelectric property, is provided simultaneously with good flexibility, can
Kynoar with piezoelectric property is compound in become in existing tissue engineering material and assign by processability and biocompatibility
Give the effective ways of tissue engineering bracket material piezoelectric property.But Kynoar is according to its processing conditions and the process of processing
The available α of difference, beta, gamma, five kinds of crystal phases of δ, ε can also be in different processing conditions and the process kind phase of processing between five kinds of crystal phases
Mutually conversion, different crystal phases have different characteristics, and only β crystal phase has piezoelectric property, and Kynoar is only in high pressure
It can just be obtained under conditions of electric field polarization or mechanical commutation draft.The Kynoar of β crystal phase can be obtained by finding one kind, and can be made
The Kynoar of β crystal phase, which is dispersed in tissue engineering bracket material, obtains that a kind of have stress active mass's engineering rack
Material and preparation method thereof becomes starting point of the invention.
Summary of the invention
In view of the above problems, the invention aims to solve to lack existing for existing tissue engineering bracket material
It falls into, provides that a kind of have stress active tissue engineering bracket material and preparation method thereof.Realize the technical scheme is that
One kind stress active mass's engineering scaffold material, it is described stress active mass's engineering scaffold material be by polyvinylidene fluoride
The cavernous body of alkene nanofiber, fibroin albumen and polyvinyl alcohol composition, the cavernous structure obtained through esterification treatment stress live
Property tissue engineering bracket material, wherein fibroin albumen and polyvinyl alcohol mass ratio are 1/9~1/1, polyvinylidene fluoride nanometer fiber
Mass ratio with polyvinyl alcohol is 1/99~5/95.
The polyvinylidene fluoride nanometer fiber is diameter 50nm-1000nm, length 5000nm-100000nm.
The fibroin albumen is the powder shaped fibroin albumen that the diameter that crushes is less than 500nm after natural silk degumming, or
After natural silk degumming, by dissolution, dialysis, the fibroin albumen being freeze-dried.
It is a kind of stress active mass's engineering scaffold material preparation method, the system including polyvinylidene fluoride nanometer tunica fibrosa
The preparation of standby, silk fibroin protein solution or fibroin albumen powder dispersion and poly-vinyl alcohol solution, which is characterized in that the preparation side
Method sequentially includes the following steps:
A. after taking the polyvinylidene fluoride nanometer tunica fibrosa that diameter is 50nm-1000nm to cut, crush ultrasonic disperse in water
Obtained diameter 50nm-1000nm, the mass concentration that length is 5000nm-100000nm are 5wt%~20wt% polyvinylidene fluoride
Alkene nanofiber dispersion liquid.
B. configured polyvinylidene fluoride nanometer fiber dispersion, silk fibroin protein solution or fibroin albumen powder dispersion are taken
It mixes to obtain uniform and stable mixing suspension with poly-vinyl alcohol solution, wherein the mass ratio of Kynoar and polyvinyl alcohol
It is 1/99~5/95, the mass ratio of fibroin albumen and polyvinyl alcohol is 5/95~50/50, Kynoar, fibroin albumen and poly-
The total soluble matters mass concentration of vinyl alcohol is 5wt%~20wt%.
C. the uniform and stable mixing suspension prepared by step b is in casting die, cryocoagulation, demoulding, and freezing is dry
It is dry, obtain the cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol.
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is handled
The cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained is in 80
DEG C, esterification and crosslinking 2-4 hours in saturation maleic acid anhydride vapours, the sealing atmosphere that percent by volume 1%-5% is HCl steam
Afterwards, take out cavernous body and be heat-treated 15-30min in 120 DEG C of baking ovens, obtain by polyvinylidene fluoride nanometer fiber, fibroin albumen with
Polyvinyl alcohol composition cavernous structure stress active mass's engineering scaffold material.
Due to using the technology described above, the present invention stress active mass's engineering scaffold material can be by people after being implanted into human body
The physical stimulations such as compression, the drawing-off that body movement generates tissue engineering bracket material are converted to polarization charge, pole by piezoelectric effect
Tissue growth and the healing between matrix will be promoted by changing charge, realize promoted using physical stimulation tissue reconstruction,
Accelerate rehabilitation course.The even porous cavernous body of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol has good
Biocompatibility and uniform pore structure provide good matrix condition for histiocytic tactophily.The present invention stress
Active mass's engineering material is polyvinylidene fluoride nanometer fiber dispersion, silk fibroin protein solution or the fibroin albumen taken water as a solvent
Powder dispersion and poly-vinyl alcohol solution mixing, eliminate in existing portion of tissue engineering material preparation process and need organic solvent
Dissolution, since organic solvent can not completely remove, remaining organic solvent is to have stronger toxicity to cell culture, breeding, is made
Later death at cell culture, breeding is serious.The preparation for not needing the tissue engineering material of organic solvent further improves
The biocompatibility of tissue engineering material.The present invention stress active mass's engineering material with polyvinylidene fluoride nanometer fiber, fibroin
Albumen and polyvinyl alcohol are material, and wherein polyvinylidene fluoride nanometer fiber has good piezoelectric effect and biocompatibility, are gathered
Vinyl alcohol is a kind of synthetic material of good biocompatibility, and natural material fibroin albumen further increases and ensure that a group weaver
The matrix condition that the biocompatibility of engineering support material, especially fibroin powder remain natural biologic material cell development, three
Compound and technique the control of kind of material becomes that have stress active tissue engineering bracket material.
The preparation method of tissue engineering bracket of the invention, due to being with polyvinylidene fluoride nanometer fiber dispersion, fibroin
Protein solution or fibroin albumen powder dispersion are mixed with poly-vinyl alcohol solution, through casting die, cryocoagulation, demoulding, freezing
The even porous cavernous body shape material being dried to obtain, it may be convenient to which organizational project material is adjusted by concentration in change mixture
The size of hole in material.Resulting mandruka body is handled using the vapour phase esterification of maleic anhydride, and making stress active group
Fibroin albumen or silk fibroin powder the bluk recombination polyvinyl alcohol knitted in engineering material do not dissolve in water, with the vapour phase esterification processing time
Extension can not only make fibroin albumen or silk fibroin powder bluk recombination polyvinyl alcohol not dissolve in the pore structure that water keeps stable, can
Further regulate and control the degradation speed of fibroin albumen or silk fibroin powder bluk recombination polyvinyl alcohol.Of the invention stress active group weaver
Engineering support material preparation method is simple and convenient to operate, is low in cost, has good controllability to preparation process, should activate
Property tissue engineering bracket material can be adapted for the tissue engineering bracket for processing Various Tissues and various shapes.
Detailed description of the invention
Fig. 1 is tissue engineering bracket material surface topography electron microscope.
Specific embodiment
Present invention is further described in detail with reference to the accompanying drawings and examples.
See attached drawing.
One kind stress active mass's engineering scaffold material, the tissue engineering bracket material be by polyvinylidene fluoride nanometer fibre
Dimension, fibroin albumen and polyvinyl alcohol composition cavernous body, the cavernous structure obtained through esterification treatment stress active group weaver
Engineering support material, wherein fibroin albumen and polyvinyl alcohol mass ratio are 1/9~1/1, polyvinylidene fluoride nanometer fiber and polyethylene
The mass ratio of alcohol is 1/99~5/95.
The polyvinylidene fluoride nanometer fiber is diameter 50nm-1000nm, length 5000nm-100000nm.
The fibroin albumen is the powder shaped fibroin albumen that the diameter that crushes is less than 500nm after natural silk degumming, or
After natural silk degumming, by dissolution, dialysis, the fibroin albumen being freeze-dried.
It is a kind of stress active mass's engineering scaffold material preparation method, the system including polyvinylidene fluoride nanometer tunica fibrosa
The preparation of standby, silk fibroin protein solution or fibroin albumen powder dispersion and poly-vinyl alcohol solution, which is characterized in that the preparation side
Method sequentially includes the following steps:
A. after taking the polyvinylidene fluoride nanometer tunica fibrosa that diameter is 50nm-1000nm to cut, crush ultrasonic disperse in water
Obtained diameter 50nm-1000nm, the mass concentration that length is 5000nm-100000nm are 5wt%~20wt% polyvinylidene fluoride
Alkene nanofiber dispersion liquid.
B. configured polyvinylidene fluoride nanometer fiber dispersion, silk fibroin protein solution or fibroin albumen powder dispersion are taken
It mixes to obtain uniform and stable mixing suspension with poly-vinyl alcohol solution, wherein the mass ratio of Kynoar and polyvinyl alcohol
It is 1/99~5/95, the mass ratio of fibroin albumen and polyvinyl alcohol is 5/95~50/50, Kynoar, fibroin albumen and poly-
The total soluble matters mass concentration of vinyl alcohol is 5wt%~20wt%.
C. the uniform and stable mixing suspension prepared by step b is in casting die, cryocoagulation, demoulding, and freezing is dry
It is dry, obtain the cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol.
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is handled
The cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained is in 80
DEG C, esterification and crosslinking 2-4 hours in saturation maleic acid anhydride vapours, the sealing atmosphere that percent by volume 1%-5% is HCl steam
Afterwards, take out cavernous body and be heat-treated 15-30min in 120 DEG C of baking ovens, obtain by polyvinylidene fluoride nanometer fiber, fibroin albumen with
Polyvinyl alcohol composition cavernous structure stress active mass's engineering scaffold material.
Silk fibroin protein solution or fibroin albumen powder dispersion in preparation step b as described above are warps after natural silk degumming
Dissolution is crossed, is dialysed, the fibroin albumen being freeze-dried is dispensed into water, and magnetic stirring is until be completely dissolved to obtain stable fibroin
Powder shaped fibroin albumen of the diameter crushed after protein solution or natural silk degumming less than 500nm is dispersed in water, ultrasound point
It dissipates and obtains the dispersion liquid of fibroin powder, the mass concentration of silk fibroin protein solution is 5wt%~20wt%；The fibroin albumen of dispersion liquid
The mass concentration of powder is 5wt%~20wt%；It is taken water as a solvent when preparing polyvinyl alcohol (PVA) solution, and the magnetic at 80 DEG C
Stirring.
Fibroin albumen powder can be prepared, silk for mulberry silk or after squeezing natural silk degumming by the method for mechanical crushing
The micro-structure of fibroin powder does not change with matrix feature；Fibroin albumen can pass through for mulberry silk or after squeezing natural silk degumming
Dissolution, dialysis, freeze-drying obtain.
Below with reference to embodiment, the invention will be further elaborated:
Embodiment one
A. take polyvinylidene fluoride nanometer tunica fibrosa cutting that diameter is 50nm, crush obtain after ultrasonic disperse in water it is straight
Diameter 50nm, the mass concentration that length is 5000nm-100000nm are 5wt% polyvinylidene fluoride nanometer fiber dispersion；
B. the polyvinylidene fluoride nanometer fiber dispersion for being 5wt% by mass concentration, mass concentration are the fibroin egg of 5wt%
The polyvinyl alcohol water solution that white water solution and mass concentration are 5wt% mixes to obtain uniform and stable mixing suspension, wherein
The mass ratio of Kynoar and polyvinyl alcohol is 1/99, and the mass ratio of fibroin albumen and polyvinyl alcohol is 5/95, polyvinylidene fluoride
The total soluble matters mass concentration of alkene, fibroin albumen and polyvinyl alcohol is 5wt%.
C. the uniform and stable mixing suspension prepared by step b is in casting die, cryocoagulation, demoulding, and freezing is dry
It is dry, obtain the cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol.
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is handled
The cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained is in 80
DEG C, in saturation maleic acid anhydride vapours, the sealing atmosphere that percent by volume 1% is HCl steam after esterification and crosslinking 2 hours, take
Cavernous body is heat-treated 15min in 120 DEG C of baking ovens out, obtains by polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol
The cavernous structure of composition stress active mass's engineering scaffold material.
Embodiment two
A. polyvinylidene fluoride nanometer tunica fibrosa cutting that diameter is 500nm is taken, crushes and obtains after ultrasonic disperse in water
Diameter 500nm, the mass concentration that length is 50000nm are 10wt% polyvinylidene fluoride nanometer fiber dispersion.
B. the polyvinylidene fluoride nanometer fiber dispersion for being 10wt% by mass concentration, mass concentration are the fibroin of 10wt%
The polyvinyl alcohol water solution that protein solution and mass concentration are 10wt% mixes to obtain uniform and stable mixing suspension,
The mass ratio of middle Kynoar and polyvinyl alcohol is 3/97, and the mass ratio of fibroin albumen and polyvinyl alcohol is 20/80, gathers inclined fluorine
The total soluble matters mass concentration of ethylene, fibroin albumen and polyvinyl alcohol is 10wt%.
C. the uniform and stable mixing suspension prepared by step b is in casting die, cryocoagulation, demoulding, and freezing is dry
It is dry, obtain the cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol.
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is handled
The cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained is in 80
DEG C, in saturation maleic acid anhydride vapours, the sealing atmosphere that percent by volume 3% is HCl steam after esterification and crosslinking 3 hours, take
Cavernous body is heat-treated 20min in 120 DEG C of baking ovens out, obtains by polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol
The cavernous structure of composition stress active mass's engineering scaffold material.
Embodiment three
A. polyvinylidene fluoride nanometer tunica fibrosa cutting that diameter is 1000nm is taken, crushes and obtains after ultrasonic disperse in water
Diameter 1000nm, the mass concentration that length is 100000nm are 20wt% polyvinylidene fluoride nanometer fiber dispersion.
B. the polyvinylidene fluoride nanometer fiber dispersion for being 20wt% by mass concentration, mass concentration are the fibroin of 15wt%
The polyvinyl alcohol water solution that protein powder dispersion liquid and mass concentration are 20wt% mixes to obtain uniform and stable mix suspending
Liquid, wherein the mass ratio of Kynoar and polyvinyl alcohol is 5/95, and the mass ratio of fibroin albumen and polyvinyl alcohol is 50/50,
The total soluble matters mass concentration of Kynoar, fibroin albumen and polyvinyl alcohol is 20wt%.
C. the uniform and stable mixing suspension prepared by step b is in casting die, cryocoagulation, demoulding, and freezing is dry
It is dry, obtain the cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol.
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is handled
The cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained is in 80
DEG C, in saturation maleic acid anhydride vapours, the sealing atmosphere that percent by volume 5% is HCl steam after esterification and crosslinking 4 hours, take
Cavernous body is heat-treated 30min in 120 DEG C of baking ovens out, obtains by polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol
The cavernous structure of composition stress active mass's engineering scaffold material.
Example IV
A. polyvinylidene fluoride nanometer tunica fibrosa cutting that diameter is 800nm is taken, crushes and obtains after ultrasonic disperse in water
Diameter 800nm, the mass concentration that length is 80000nm are 15wt% polyvinylidene fluoride nanometer fiber dispersion.
B. the polyvinylidene fluoride nanometer fiber dispersion for being 15wt% by mass concentration, mass concentration are the fibroin of 20wt%
The polyvinyl alcohol water solution that protein powder dispersion liquid and mass concentration are 5wt% mixes to obtain uniform and stable mix suspending
Liquid, wherein the mass ratio of Kynoar and polyvinyl alcohol is 5/95, and the mass ratio of fibroin albumen and polyvinyl alcohol is 1/1, is gathered
The total soluble matters mass concentration of vinylidene, fibroin albumen and polyvinyl alcohol is 5wt%.
C. the uniform and stable mixing suspension prepared by step b is in casting die, cryocoagulation, demoulding, and freezing is dry
It is dry, obtain the cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol.
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is handled
The cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained is in 80
DEG C, in saturation maleic acid anhydride vapours, the sealing atmosphere that percent by volume 4% is HCl steam after esterification and crosslinking 3 hours, take
Cavernous body is heat-treated 25min in 120 DEG C of baking ovens out, obtains by polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol
The cavernous structure of composition stress active mass's engineering scaffold material.
Claims (1)
1. one kind stress active mass's engineering scaffold material preparation method, including diameter be 50nm-1000nm Kynoar
The preparation of nano fibrous membrane, the preparation of silk fibroin protein solution or fibroin albumen powder dispersion and poly-vinyl alcohol solution, feature
It is, the preparation method sequentially includes the following steps:
A. polyvinylidene fluoride nanometer tunica fibrosa cutting that diameter is 50nm-1000nm is taken, crushes and obtains after ultrasonic disperse in water
Diameter 50nm-1000nm, the mass concentration that length is 5000nm-100000nm is that 5wt%~20wt% Kynoar is received
Rice fiber dispersion；
B. configured polyvinylidene fluoride nanometer fiber dispersion, silk fibroin protein solution or fibroin albumen powder dispersion are taken and is gathered
Glycohol solution mixes to obtain uniform and stable mixing suspension, and wherein the mass ratio of Kynoar and polyvinyl alcohol is 1/
99~5/95, the mass ratio of fibroin albumen and polyvinyl alcohol is 5/95~50/50, Kynoar, fibroin albumen and polyethylene
The total soluble matters mass concentration of alcohol is 5wt%~20wt%；
C. the uniform and stable mixing suspension prepared by step b is in casting die, cryocoagulation, demoulds, and freeze-drying obtains
To the cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol；
D. the cavernous body vapour phase esterification processing of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition obtains step c
To the cavernous body being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol in 80 DEG C, be saturated maleic acid
Anhydride vapours, percent by volume 1%-5% be HCl steam sealing atmosphere in after esterification and crosslinking 2-4 hour, taking-up cavernous body in
It is heat-treated 15-30min in 120 DEG C of baking ovens, obtains the sea being made of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol
Continuous body structure stress active mass's engineering scaffold material.
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| CN110694115B (en) * | 2019-10-22 | 2022-03-01 | 上海交通大学医学院附属第九人民医院 | Method for constructing tendon tissue in vitro, and biological material and application thereof |
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