CN106386820A - Pesticide composition containing 2-methylsulfonyl-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and tebuconazole - Google Patents

Pesticide composition containing 2-methylsulfonyl-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and tebuconazole Download PDF

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Publication number
CN106386820A
CN106386820A CN201610793024.6A CN201610793024A CN106386820A CN 106386820 A CN106386820 A CN 106386820A CN 201610793024 A CN201610793024 A CN 201610793024A CN 106386820 A CN106386820 A CN 106386820A
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China
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tebuconazole
bacterium azoles
dichloro
agent
dislikes bacterium
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Inventor
和立连
韦庆书
邓明娟
蓝宏彦
谭仁景
梁永星
谭仁卫
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LIUZHOU HUINONG CHEMICAL CO Ltd
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LIUZHOU HUINONG CHEMICAL CO Ltd
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Priority to CN201610793024.6A priority Critical patent/CN106386820A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a pesticide composition containing 2-methylsulfonyl-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and tebuconazole. The pesticide composition comprises the following active components by weight: 1 to 30 parts of 2-methylsulfonyl-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and 1 to 50 parts of tebuconazole. The pesticide composition can overcome and delay resistance of pathogens, has better protection effect compared with individual usage of the active components, is resistant to rain wash, good in fast action performance and long in acting period, reduces usage frequency, saves time and labor, prevents and treats a plurality of pests and diseases at the same time and lowers the usage cost of pesticide.

Description

A kind of composition pesticide disliking bacterium azoles and Tebuconazole containing dichloro
Technical field
The invention belongs to pesticide field is and in particular to a kind of composition pesticide disliking bacterium azoles and Tebuconazole containing dichloro.
Background technology
Due to agricultural chemicals single using and medication not science, unreasonable, rapidly increased pathogen to currently used The resistance of agricultural chemicals is so that high cost, long-time some the agricultural chemicals new components developed that put into shorten service life, simultaneously using secondary Number is many, has also increased environmental pollution and has increased drug cost.Citrus psylla is unique medium that yellow twig is propagated, and has tender in the same year In the case of the tip, can occur 11～14 generations for 1 year, field generations are overlapping, single wood louse passes sick rate and is up to 70-80%, and adult can Fly, difficulty of prevention and cure is high.Meanwhile, in each growth period of oranges and tangerines, except wood louse, also red spider, blister mite, leaf miner, aleyrodid, Occur while the disease such as the insect pests such as aphid and resin disease (rubber disease), anthracnose, so such pest and disease damage in the urgent need to address Problem.At present for the control of insect being agriculturally also easy to produce resistance, main method is exploitation and existing kind no interactions resistance Compounding between new composition or existing pesticide species, the former is because required development cost is high, the cycle is long, and develops speed at present Degree is much less than the speed that pest resistance to insecticide produces.
Dichloro dislikes bacterium azoles：It is a kind of azoles sulfone series bactericidal agent, chemical name is：2- methyl sulfuryl -5- (2,4 dichloro benzene Base) -1,3,4- diazole, molecular structure isIts molecular formula is C9H6Cl2N2O3S, divides relatively Protonatomic mass is 293.1.It is a kind of new type bactericide, be low toxicity, the green chemical pesticide of safety, but this agricultural chemicals is not also extensive Use.Physicochemical property：Active compound is acicular crystal, water insoluble, is soluble in conventional organic solvent.Active compound to light, thermally-stabilised, in alkali Easily decompose under property condition (pH10).Mammalian toxicity：Rat acute LD50=1780mg/kg.Rat acute is percutaneous and inhales Enter LD50>2000mg/kg.
Tebuconazole, this product is clear crystal, and fusing point is 102.4 DEG C, vapour pressure 0.0133mPa (20 DEG C)：Solubility (20 ℃)：Water 32mg/L, toluene 50-100g/L.Tebuconazole is a kind of efficient, wide spectrum, absorbability triazole type sterilization pesticide, has guarantor Protect, treat, rooting out three zones, wide sterilization spectrum, lasting period are long.As all of triazole bactericidal agent, Tebuconazole can press down The biosynthesis of the ergosterol of fungi processed.It is the efficient sterilizing of Seed Treatment for Important Economic crop or foliage spray Agent, can prevent and treat multiple rust of cereal crop, powdery mildew, net blotch, root rot, head blight effectively, and smut and planting passes Zonate spot and Rice shealth blighy etc..
Above-mentioned citrus red mite, oranges and tangerines blister mite, oranges and tangerines resin disease, citrus anthracnose, oranges and tangerines downy mildew, c itrus canker Disease, oranges and tangerines gray mold, orange caterpillar, diaphorina citri create certain resistance to the action of a drug to Tebuconazole, are used alone and do not reach reason The prevention effect thought.At present, we not yet find and for dichloro to dislike Patents and the document report that bacterium azoles and Tebuconazole are applied in combination Road.
Content of the invention
The purpose of the present invention solves above-mentioned technical problem, provides a kind of pesticide combination disliking bacterium azoles and Tebuconazole containing dichloro Thing, single dose, resistance of rainwater washing against, quick-acting is good, the lasting period is long to overcome and delay the pathogen resistance to the action of a drug, preventive effect to be better than, and reduces Access times, save time, laborsaving, can prevent and treat various pests and disease simultaneously, reduce drug cost.
For realizing above-mentioned purpose, the technical scheme is that：
A kind of composition pesticide disliking bacterium azoles and Tebuconazole containing dichloro, including active component, described active component is dichloro Dislike bacterium azoles and Tebuconazole, be calculated by weight as：
Dichloro dislikes bacterium azoles 1-30 part；
Tebuconazole 1-50 part.
As further technical scheme, it is 1-5 part that the above dichloro dislikes bacterium azoles, and described Tebuconazole is 1-50 part.
As further technical scheme, it is 1-20 part that the above dichloro dislikes bacterium azoles, and described Tebuconazole is 1-30 part.
As further technical scheme, it is 1-15 part that the above dichloro dislikes bacterium azoles, and described Tebuconazole is 1-30 part.
As further technical scheme, it is 1-30 part that the above dichloro dislikes bacterium azoles, and described Tebuconazole is 1-30 part.
As further technical scheme, above composition pesticide also includes auxiliary agent, and described auxiliary agent includes dispersant, emulsification One or more of agent, antifreezing agent, thickener, defoamer, solvent, wetting agent, disintegrant and filler.
As further technical scheme, the parts by weight that the above auxiliary agent adds are：
As further technical scheme, the above emulsifying agent is calcium dodecyl benzene sulfonate, dodecyl polyoxy second Alkene ether, styrylphenol polyoxyethylene ether, NPE, thermally coupled distillation columns, fatty acid polyglycol In oxyethylene group ester, polyoxyethylene aliphatic alcohol ether, dodecyl polyoxyethylene sulfonic acid sodium salt and phenethyl phenol polyethenoxy ether One or more；
The above solvent is dimethylbenzene, diesel oil, toluene, cyclohexanol, methyl alcohol, soybean oil, turpentine oil, methyl oleate, molten One of agent oil, dimethylformamide, ethanol, N- pyrrolidones, ethyl acetate and water or two or more combinations；
The above wetting agent is lauryl sodium sulfate, calcium dodecyl benzene sulfonate, methyl naphthalene sulfonic acid sodium formaldehyde are condensed One of thing, pull open powder, Negel and spaonin powder or two or more combinations；
The above dispersant is sodium lignin sulfonate, calcium lignosulfonate, APES formaldehyde condensation product Sodium sulphate, calcium dodecyl benzene sulfonate, APES, polycarboxylic acids sodium, aliphatic amine polyoxyethylene ether and glycerine polyoxy One of ethene ether fatty acid ester or two or more combinations；
The above antifreezing agent is one of ethylene glycol, propane diols, glycerine and urea or two or more combinations；
The above thickener is one of xanthans, Magnesiumaluminumsilicate, hydroxymethyl cellulose and polyvinyl alcohol or two kinds Above combination；
The above defoamer is one of silicone oil, organic silicone and polypropylene glycerol aether or two or more groups Close；
The above disintegrant be one of bentonite, ammonium sulfate, sodium acid carbonate, aluminium chloride and succinic acid or two kinds with On combination；
The above filler is one of white carbon, kaolin, diatomite, precipitated calcium carbonate and starch or two or more Combination.
As further technical scheme, the formulation of above composition pesticide is suspending agent, aqueous emulsion, water dispersible granules, Microemulsion or wettable powder.
A kind of preparation method of the composition pesticide disliking bacterium azoles and Tebuconazole containing dichloro as above, it is to become activity Divide and auxiliary agent mixes by weight, can get by the conventional machining mode of its formulation.
A kind of purposes of the composition pesticide disliking bacterium azoles and Tebuconazole containing dichloro as above, it is red in preventing and treating oranges and tangerines Spider, oranges and tangerines blister mite, oranges and tangerines resin disease, citrus anthracnose, oranges and tangerines downy mildew, citrus bacterial canker disease, oranges and tangerines gray mold, oranges and tangerines aphid Application in worm, diaphorina citri.
The component of the present invention is commercially available prod.
Compared with prior art, the device have the advantages that being：Tebuconazole in the present invention is due to time of application relatively Early, citrus red mite, oranges and tangerines blister mite, oranges and tangerines resin disease, citrus anthracnose, oranges and tangerines downy mildew, citrus bacterial canker disease, oranges and tangerines grey mold Disease, orange caterpillar, diaphorina citri have created stronger drug resistance to it；Dichloro dislike bacterium azoles to soil-borne disease and migrate disease and Other fungal diseases have good inhibiting effect, it has been investigated that, dichloro dislikes bacterium azoles and Tebuconazole with mass ratio 1:50～30: 1 compounds, and can produce higher synergistic effect, expands broad-spectrum, overcomes and delay pathogen to develop immunity to drugs.Preventive effect is better than single Agent, resistance of rainwater washing against, quick-acting are good, the lasting period is long, reduce access times, save time, laborsaving, can prevent and treat simultaneously multiple pathogens with And disease, reduce drug cost.Especially to citrus red mite, oranges and tangerines blister mite, oranges and tangerines resin disease, citrus anthracnose, oranges and tangerines Downy mildew, citrus bacterial canker disease, oranges and tangerines gray mold, orange caterpillar, diaphorina citri have obvious synergistic effect, and prevention effect is good.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to The scope that embodiment represents.
Embodiment 1：50% Tebuconazole dichloro dislikes bacterium azole suspending agent
Embodiment 2：2% Tebuconazole dichloro dislikes bacterium azole suspending agent
Embodiment 3：60% Tebuconazole dichloro dislikes bacterium azole suspending agent
Embodiment 4：40% Tebuconazole dichloro dislikes bacterium azole suspending agent
Embodiment 5：30% Tebuconazole dichloro dislikes bacterium azole suspending agent
The preparation method of embodiment 1-5 is：Proportioning according to each embodiment weighs after raw material, and raw material, solvent mixing are stirred Mix dissolving, add the auxiliary agents such as emulsification, dispersion to carry out high speed shearing emulsification, make corresponding dichloro and dislike the suspension of bacterium azoles Tebuconazole Agent.
Embodiment 6：40% Tebuconazole dichloro dislikes bacterium azoles aqueous emulsion
Embodiment 7：2% Tebuconazole dichloro dislikes bacterium azoles aqueous emulsion
Embodiment 8：60% Tebuconazole dichloro dislikes bacterium azoles aqueous emulsion
Embodiment 9：40% Tebuconazole dichloro dislikes bacterium azoles aqueous emulsion
Embodiment 10：30% Tebuconazole dichloro dislikes bacterium azoles aqueous emulsion
The preparation method of embodiment 6-10 is：Proportioning according to each embodiment weighs after raw material, and raw material, solvent mixing are stirred Mix dissolving, add emulsifying agent to carry out high speed shearing emulsification, make corresponding Tebuconazole dichloro and dislike bacterium azoles aqueous emulsion.
Embodiment 11：25% Tebuconazole dichloro dislikes bacterium azoles water dispersible granules
Embodiment 12：2% Tebuconazole dichloro dislikes bacterium azoles water dispersible granules
Embodiment 13：55% Tebuconazole dichloro dislikes bacterium azoles water dispersible granules
Embodiment 14：55% Tebuconazole dichloro dislikes bacterium azoles water dispersible granules
Embodiment 15：40% Tebuconazole dichloro dislikes bacterium azoles water dispersible granules
The preparation method of embodiment 11-15 is：Proportioning according to each embodiment weighs after raw material, and raw material mixed powder is broken, Make corresponding Tebuconazole dichloro and dislike bacterium azoles water dispersible granules through granulation, drying.
Embodiment 16：30% Tebuconazole dichloro dislikes bacterium azoles wettable powder
Embodiment 17：2% Tebuconazole dichloro dislikes bacterium azoles wettable powder
Embodiment 18：60% Tebuconazole dichloro dislikes bacterium azoles wettable powder
Embodiment 19：65% Tebuconazole dichloro dislikes bacterium azoles wettable powder
Embodiment 20：40% Tebuconazole dichloro dislikes bacterium azoles wettable powder
The preparation method of embodiment 16-20 is：Proportioning according to each embodiment weighs after raw material, by raw material mixing Ultramicro-powder Broken, make corresponding Tebuconazole dichloro and dislike bacterium azoles wettable powder.
Embodiment 21：40% Tebuconazole dichloro dislikes bacterium azoles microemulsion
Embodiment 22：10% Tebuconazole dichloro dislikes bacterium azoles microemulsion
Embodiment 23：60% Tebuconazole dichloro dislikes bacterium azoles microemulsion
Embodiment 24：40% Tebuconazole dichloro dislikes bacterium azoles microemulsion
Embodiment 25：30% Tebuconazole dichloro dislikes bacterium azoles microemulsion
The preparation method of embodiment 20-25 is：Proportioning according to each embodiment weighs after raw material, by raw material, solvent mixing Stirring and dissolving, adds emulsifying agent, water to carry out high speed shear, makes corresponding Tebuconazole dichloro and dislikes bacterium azoles microemulsion.
Application Example：
In order to detect the insecticidal effect of the composition of the present invention, now enumerate following experimental data：
, all by the conventional spray requirement of this area, that is, leaf is being just for the fountain height to the medicament dilution of table 10 for the table 1 below The back side, fruit surface are sprayed to formation drop-wise and flow down.
Application Example 5
The 30% Tebuconazole dichloro that the embodiment of the present invention 5 is obtained dislike bacterium azole suspending agent and 25% Tebuconazole missible oil, 20% dichloro is disliked bacterium azole suspending agent and is carried out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- group of navel orange of Guilin Liang Feng Orchard, spray-on process, adopt double dilution method during preparating liquid, uniformly spray in oranges and tangerines branches and leaves and fruit, first in resin disease morbidity Phase carries out first time dispenser, applies altogether 4 times, is investigated within 20 days after last medicine, and (every cell is for 2～3 plants with cell calculation for investigation area Mandarin tree), every cell investigates 2 plants of trees, every plant press east, south, west, north, in five points samplings, two branches of every investigation, each Branch investigates top down 4 leaves and whole fruit, and total leaf (really) of record investigation is several, sick leaf (really) counts and sick level, calculates disease Feelings index and preventive effect.
Application Example 5 result of the test such as table 1：
Table 1 30% Tebuconazole dichloro dislikes bacterium azole suspending agent preventing and treating oranges and tangerines resin disease (rubber disease) test of pesticide effectiveness result
Table 1 result of the test shows, starts to apply first time medicine in oranges and tangerines resin disease (rubber disease) early period of origination, is thanking to colored back seat Fruit middle and later periods, young fruit period, fruit growth period are respectively applied once again, and 30% Tebuconazole dichloro dislikes bacterium azole suspending agent preventing and treating oranges and tangerines resin Disease (rubber disease), by 1500 times of dilution sprayings, has preferable preventive effect.
Application Example 7
The 2% Tebuconazole dichloro that the embodiment of the present invention 7 is obtained dislike bacterium azoles aqueous emulsion and 25% Tebuconazole missible oil, 20% Dichloro is disliked bacterium azole suspending agent and is carried out field control effectiveness test, and test site is mono- group of navel orange fruit of Guilin City Guangxi land reclamation and cultivation Guilin Liang Feng Garden, foliar spray method, it is watered dilution by process dosage and is made into liquid, uniformly spray in oranges and tangerines leaf tow sides, in oranges and tangerines Aphid is contained and sends out phase dispenser 1 time, investigates insect population shortly before dispenser, 1 day after dispenser, respectively investigates borer population of once surviving, altogether within 3 days, 7 days 3 times.Investigation area calculates (every cell is for 2～3 plants of mandarin trees) with cell, and every cell investigates 2 mandarin trees, every plant by east, south, West, north, in 5 orientation investigate 5 killed tips, every tip investigates the aphid number of 5 leaves of top tip, calculates relative control effect.
Application Example 7 result of the test such as table 2：
Table 2 2% Tebuconazole dichloro dislikes bacterium azoles aqueous emulsion preventing and treating orange caterpillar test of pesticide effectiveness result
Table 2 result of the test shows, orange caterpillar contain send out a phase dispenser, 2% Tebuconazole dichloro dislike bacterium azoles aqueous emulsion press with Upper usage amount spray, has preferable preventive effect to preventing and treating orange caterpillar.
Application Example 15
The 40% Tebuconazole dichloro that the embodiment of the present invention 15 is obtained dislikes bacterium azoles water dispersible granules and 25% Tebuconazole breast Oil, 20% dichloro are disliked bacterium azole suspending agent and are carried out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation Guilin Liang Feng mono- team Navel orange orchard, foliar spray method, it is watered dissolved dilution by process dosage and is made into liquid, whole strain is sprayed to mandarin tree, Orange young is taken out and is sent out phase dispenser 1 time, investigates wood louse insect population shortly before dispenser, 5 days after dispenser, respectively investigates once within 10 days, 15 days Survival borer population, totally 3 times.Investigation area calculates (every cell is for 2～3 plants of mandarin trees) with cell, and investigation method is determined the tip using singling and adjusted Look into, every cell investigates 2 mandarin trees, every plant by east, south, west, north, in 5 different azimuth are each chooses 2 and have the worm tip to enter rower Note, investigation records every tip work borer population.Compare with blank district, calculate relative control effect.
Application Example 15 prevents and treats diaphorina citri result of the test such as table 3：
Table 3 40% Tebuconazole dichloro is disliked bacterium azoles water dispersible granules and is prevented and treated diaphorina citri test of pesticide effectiveness result
Table 3 result of the test shows, endangers phase dispenser in diaphorina citri, and 40% Tebuconazole dichloro dislikes bacterium azoles water dispersible granules In the case of amount used above, there is preferable preventive effect to preventing and treating diaphorina citri.
The 40% Tebuconazole dichloro that the embodiment of the present invention 15 is obtained dislikes bacterium azoles water dispersible granules and 25% Tebuconazole breast Oil, 20% dichloro are disliked bacterium azole suspending agent and are carried out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation Guilin Liang Feng mono- team Navel orange orchard, foliar spray method, it is watered dilution by process dosage and is made into liquid, uniformly spray in oranges and tangerines leaf tow sides, Contain in orange caterpillar and send out phase dispenser 1 time, shortly before dispenser, investigate insect population, 1 day after dispenser, 3 days, each investigation in 7 days once survives Borer population, totally 3 times.Investigation area calculates (every cell is for 2～3 plants of mandarin trees) with cell, and every cell investigates 2 mandarin trees, presses for every plant East, south, west, north, in 5 orientation investigate 5 killed tips, every tip investigates the aphid number of 5 leaves of top tip, calculates relative control effect.
Application Example 15 prevents and treats orange caterpillar result of the test such as table 4：
Table 4 40% Tebuconazole dichloro dislikes bacterium azoles water dispersible granules preventing and treating orange caterpillar test of pesticide effectiveness result
Table 4 result of the test shows, contains in orange caterpillar and sends out phase dispenser, and 40% Tebuconazole dichloro dislikes bacterium azoles water dispersible granules In the case of amount used above, there is preferable preventive effect to preventing and treating orange caterpillar.
Application Example 16
The 30% Tebuconazole dichloro that the embodiment of the present invention 16 is obtained dislikes bacterium azoles wettable powder and 25% Tebuconazole breast Oil, 20% dichloro are disliked bacterium azole suspending agent and are carried out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation Guilin Liang Feng mono- team Navel orange orchard, foliar spray method, it is watered dilution by process dosage and is made into liquid, uniformly spray in mandarin tree blade, branch extremely Moistening, in oranges and tangerines blister mite peak of occurrence early stage dispenser 1 time, investigate, shortly before dispenser, mite quantity of living, 1 day after dispenser, 7 days, 14 days, 21 days, 28 days each remaining mite numbers alive investigating once fixed point leaf, totally 5 times.(every cell is for 2～3 plants of mandarin oranges with cell calculation for investigation area Tangerine), investigation method is that every cell investigates 2 mandarin trees, every plant press east, south, west, north, in 5 azimuth mark side shoots, investigation The mite quantity alive of 20 new leaf backs, directly observes the mite number in each visual field with 10 times of folding hand held magnascopes.Right with blank Compare according to area, calculate relative control effect.
Application Example 16 prevents and treats oranges and tangerines blister mite result of the test such as table 5：
Table 5 30% Tebuconazole dichloro dislikes bacterium azoles wettable powder preventing and treating oranges and tangerines blister mite test of pesticide effectiveness result
Table 5 result of the test shows, in oranges and tangerines blister mite peak of occurrence early stage dispenser, 30% Tebuconazole dichloro is disliked bacterium azoles and existed In the case of amount used above, there is preferable preventive effect to wettable powder preventing and treating oranges and tangerines blister mite.
The 30% Tebuconazole dichloro that the embodiment of the present invention 16 is obtained dislikes bacterium azoles wettable powder and 25% Tebuconazole breast Oil, 20% dichloro are disliked bacterium azole suspending agent and are carried out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation Guilin Liang Feng mono- team Navel orange orchard, foliar spray method, it is watered dilution by process dosage and is made into liquid, uniformly spray in mandarin tree blade positive and negative two Face, limb, to moistening, contained in citrus red mite and are sent out initial stage dispenser 1 time, investigate, shortly before dispenser, mite quantity of living, 15 days after dispenser, 20 My god, each investigation in 30 days once pinpoint the remaining red spider quantity of leaf, totally 3 times.(every cell is for 2～3 plants with cell calculation for investigation area Mandarin tree), investigation method is that every cell investigates 2 mandarin trees, tree crown press east, south, west, north, in each mark in 5 orientation one Treetop branch, 5 blades on investigation every tip top, investigate remaining red spider quantity on 25 blades altogether, with 10 times of collapsible hands Hold the mite number that each visual field directly observed by magnascope.Compare with blank district, calculate relative control effect.
Application Example 16 prevents and treats citrus red mite result of the test such as table 6：
Table 6 30% Tebuconazole dichloro dislikes bacterium azoles wettable powder preventing and treating citrus red mite test of pesticide effectiveness result
Table 6 result of the test shows, contains in citrus red mite and sends out initial stage dispenser, and 30% Tebuconazole dichloro dislikes bacterium azoles wettable Pulvis, in the case of amount used above, has preferable preventive effect to preventing and treating citrus red mite.
Application Example 22
The 10% Tebuconazole dichloro that the embodiment of the present invention 22 is obtained dislike bacterium azoles microemulsion and 25% Tebuconazole missible oil, 20% dichloro is disliked bacterium azole suspending agent and is carried out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- group of navel orange of Guilin Liang Feng Orchard, foliar spray method, it is watered dissolved dilution by process dosage and is made into liquid, whole strain is sprayed, in oranges and tangerines to mandarin tree Young sprout is taken out and is sent out phase dispenser 1 time, investigates insect population shortly before dispenser, 5 days after dispenser, respectively investigates borer population of once surviving within 10 days, 15 days, Totally 3 times.Investigation area calculates (every cell is for 2～3 plants of mandarin trees) with cell, and investigation method determines tip investigation, every cell using singling 2 mandarin trees of investigation, every plant by east, south, west, north, in 5 different azimuth are each chooses 2 and have the worm tip to be marked, investigation records Every tip work borer population.Compare with blank district, calculate relative control effect.
Application Example 22 result of the test such as table 7：
Table 7 10% Tebuconazole dichloro is disliked bacterium azoles microemulsion and is prevented and treated diaphorina citri test of pesticide effectiveness result
Table 7 result of the test shows, causes harm phase dispenser in diaphorina citri, 10% Tebuconazole dichloro dislike bacterium azoles microemulsion with In the case of upper usage amount, there is preferable preventive effect to preventing and treating diaphorina citri.
Application Example 6
The 40% Tebuconazole dichloro that the embodiment of the present invention 6 is obtained dislike bacterium azoles aqueous emulsion and 25% Tebuconazole missible oil, 20% dichloro is disliked bacterium azole suspending agent and is carried out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- group of navel orange of Guilin Liang Feng Orchard, using spray-on process, adopts double dilution method, uniformly sprays in oranges and tangerines branches and leaves and fruit, send out in downy mildew during preparating liquid The sick initial stage carries out first time dispenser, applies 4 times altogether.Investigated within 20 days after last medicine.(every cell is for 2 with cell calculation for investigation area ～3 plants of mandarin trees), every cell investigates 2 plants of trees, every plant press east, south, west, north, in five points samplings, two branches of every investigation, Each branch investigates top down 4 leaves and whole fruit, and total leaf (really) of record investigation is several, sick leaf (really) counts and sick level, meter Calculate disease index and preventive effect.
Application Example 6 downy mildew) result of the test such as table 8：
Table 8 40% Tebuconazole dichloro dislikes bacterium azoles aqueous emulsion preventing and treating oranges and tangerines downy mildew test of pesticide effectiveness result
Table 8 result of the test shows, starts to apply first time medicine in oranges and tangerines downy mildew early period of origination, thank spend after in setting after Phase, young fruit period, fruit growth period are respectively applied once again, and 40% Tebuconazole dichloro dislikes what bacterium azoles aqueous emulsion was tested in amount used above In the case of, there is preferable preventive effect to preventing and treating oranges and tangerines downy mildew.
The 40% Tebuconazole dichloro that the embodiment of the present invention 6 is obtained dislike bacterium azoles aqueous emulsion and 25% Tebuconazole missible oil, 20% dichloro is disliked bacterium azole suspending agent and is carried out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- group of navel orange of Guilin Liang Feng Orchard, using spray-on process, adopts double dilution method during preparating liquid, uniformly spray in oranges and tangerines branches and leaves and fruit, in oranges and tangerines anthrax Sick early stage or premorbid carry out first time dispenser, apply altogether 3 times, and dispenser is spaced 10 days, carry out within 15 days after last medicine disposable Investigation, investigation area calculates (every cell is for 2～3 plants of mandarin trees) with cell.
Blade disease survey method：Every cell investigates 2 plants of trees, every plant press east, south, west, north, in five points samplings, every point Whole blades of two tips of investigation, total leaf tree of record investigation, sick leaf tree and sick level, calculate disease index and preventive effect.
Fruit disease investigation method：Every cell investigates two plants of mandarin trees, 100 fruits of every plant of random searching, record investigation Total fruit tree and disease fruit number, calculate diseased fruit rate and preventive effect.
Application Example 6 prevents and treats citrus anthracnose result of the test such as table 9：
Table 9 40% Tebuconazole dichloro dislikes bacterium azoles aqueous emulsion preventing and treating citrus anthracnose test of pesticide effectiveness result
Table 9 result of the test shows, carries out first time dispenser in citrus anthracnose early stage or premorbid, young fruit period, really Grow directly from seeds and respectively apply once for a long time, 40% Tebuconazole dichloro dislikes bacterium azoles aqueous emulsion in the case that above dosage is tested, to anti- Controlling citrus anthracnose has preferable preventive effect.
Application Example 11
The 25% Tebuconazole dichloro that the embodiment of the present invention 11 is obtained dislikes bacterium azoles water dispersible granules and 25% Tebuconazole breast Oil, 20% dichloro are disliked bacterium azole suspending agent and are carried out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation Guilin Liang Feng mono- team Navel orange orchard, foliar spray method, it is watered dilution by process dosage and is made into liquid, uniformly spray in mandarin tree blade, branch extremely Moistening, in oranges and tangerines blister mite peak of occurrence early stage dispenser 1 time, investigate, shortly before dispenser, mite quantity of living, 1 day after dispenser, 7 days, 14 days, 21 days, 28 days each remaining mite numbers alive investigating once fixed point leaf, totally 5 times.(every cell is for 2～3 plants of mandarin oranges with cell calculation for investigation area Tangerine), investigation method is that every cell investigates 2 mandarin trees, every plant press east, south, west, north, in 5 azimuth mark side shoots, investigation The mite quantity alive of 20 new leaf backs, directly observes the mite number in each visual field with 10 times of folding hand held magnascopes.Right with blank Compare according to area, calculate relative control effect.
Application Example 11 prevents and treats oranges and tangerines blister mite result of the test such as table 10：
Table 10 25% Tebuconazole dichloro dislikes bacterium azoles water dispersible granules preventing and treating oranges and tangerines blister mite test of pesticide effectiveness result
Table 10 result of the test shows, in oranges and tangerines blister mite peak of occurrence early stage dispenser, 25% Tebuconazole dichloro dislikes bacterium azoles Water dispersible granules, in the case that amount used above is tested, have preferable preventive effect to preventing and treating oranges and tangerines blister mite.
Application example embodiment 14
The 55% Tebuconazole dichloro that the embodiment of the present invention 14 is obtained dislikes bacterium azoles water dispersible granules and 25% Tebuconazole breast Oil, 20% dichloro are disliked bacterium azole suspending agent and are carried out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation Guilin Liang Feng mono- team Navel orange orchard, using spray-on process, adopts double dilution method during preparating liquid, uniformly spray in oranges and tangerines branches and leaves and fruit, in oranges and tangerines Canker early stage or premorbid carry out first time dispenser, apply altogether 3 times, and dispenser is spaced 10 days, carries out one within 15 days after last medicine Secondary sex investigation, investigation area calculates (every cell is for 2～3 plants of mandarin trees) with cell.
Blade disease survey method：Every cell investigates 2 plants of trees, every plant press east, south, west, north, in five points samplings, every point Whole blades of two tips of investigation, total leaf tree of record investigation, sick leaf tree and sick level, calculate disease index and preventive effect.
Fruit disease investigation method：Every cell investigates two plants of mandarin trees, 100 fruits of every plant of random searching, record investigation Total fruit tree and disease fruit number, calculate diseased fruit rate and preventive effect.
Application Example 14 prevents and treats citrus bacterial canker disease result of the test such as table 11：
Table 11 55% Tebuconazole dichloro dislikes bacterium azoles aqueous emulsion preventing and treating citrus bacterial canker disease test of pesticide effectiveness result
Table 11 result of the test shows, carries out first time dispenser in citrus bacterial canker disease early stage or premorbid, young fruit period, really Grow directly from seeds and respectively apply once for a long time, 55% Tebuconazole dichloro dislikes bacterium azoles water dispersible granules in the case that above dosage is tested, There is preferable preventive effect to preventing and treating citrus bacterial canker disease.
Application example embodiment 19
The 65% Tebuconazole dichloro that the embodiment of the present invention 19 is obtained dislikes bacterium azoles wettable powder and 25% Tebuconazole breast Oil, 20% dichloro are disliked bacterium azole suspending agent and are carried out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation Guilin Liang Feng mono- team Navel orange orchard, using spray-on process, adopts double dilution method during preparating liquid, uniformly spray in oranges and tangerines branches and leaves and fruit, in oranges and tangerines Gray mold early stage or premorbid carry out first time dispenser, apply altogether 3 times, and dispenser is spaced 10 days, carries out one within 15 days after last medicine Secondary sex investigation, investigation area calculates (every cell is for 2～3 plants of mandarin trees) with cell.
Blade disease survey method：Every cell investigates 2 plants of trees, every plant press east, south, west, north, in five points samplings, every point Whole blades of two tips of investigation, total leaf tree of record investigation, sick leaf tree and sick level, calculate disease index and preventive effect.
Fruit disease investigation method：Every cell investigates two plants of mandarin trees, 100 fruits of every plant of random searching, record investigation Total fruit tree and disease fruit number, calculate diseased fruit rate and preventive effect.
Application Example 19 prevents and treats oranges and tangerines gray mold result of the test such as table 12：
Table 12 65% Tebuconazole dichloro dislikes bacterium azoles wettable powder preventing and treating oranges and tangerines gray mold test of pesticide effectiveness result
Table 12 result of the test shows, carries out first time dispenser in oranges and tangerines gray mold early stage or premorbid, young fruit period, really Grow directly from seeds and respectively apply once for a long time, 65% Tebuconazole dichloro dislikes bacterium azoles wettable powder in the case that above dosage is tested, There is preferable preventive effect to preventing and treating oranges and tangerines gray mold.
Following instance illustrates the synergistic effect of composition
The Toxicity Determination to diaphorina citri for the pesticide sterilizing Pesticidal combination
Subjects are diaphorina citri.
Test adopts medicine embrane method.Take cylindrical transparent glass preserving jar (base diameter 6.8cm, bottleneck diameter 5.5cm, height 8.7cm), add 50ml to be diluted to the liquid of desired concn, cover tightly lid, gently shake up, be inverted 30s, make bottle inwall all viscous Then liquid is poured out, then allows bottle naturally dry by full liquid.Oranges and tangerines tender shoots is soaked 1min with identical liquid, naturally dries in the air Put in the bottle having dried after dry；Encase tender shoots base portion moisturizing with the cotton of dipped identical liquid.Wood louse adult band plug glass Test tube gathers from kamuning tree.Each process is repeated 3 times, and repeats 10 adults every time.CO is used during process2Anesthesia (18-20s) After put into each process bottle in, blank clear water, each bottleneck is covered with 40 mesh gauzes, in case adult flies out.20 minutes After check whether there is dead worm, borer population of living before record medicine.The vial after worm will be connect it will be placed in the illumination box of temperature (25 ± 1) DEG C In.After medicine, 24h estimates dead borer population, borer population of living, and calculates the death rate, it is efficiency test that the clear water comparison death rate is less than 10%.
First concentration conversion is become natural logrithm value, corrected mortality is converted into arcsine transformation value.Again with arcsine transformation It is worth for dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Software, draw each The toxicity regression linear equation of individual medicament.Calculate toxicity index and the co-toxicity coefficient (CTC) of medicament according to the abundant method of Sun Yun.
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content in mixture for the toxicity index × single dose
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Experimental result such as table 13：
Table 13：The toxicity index of each medicament and the co-toxicity coefficient of Tebuconazole dichloro evil bacterium azoles
Table 13 shows that Tebuconazole dislikes bacterium azoles in mass ratio 50 with dichloro：1～1：30 compound, and diaphorina citri are all had synergy make With especially proportioning is 1:5～40:When between 1, synergy is more notable, and co-toxicity coefficient is all higher than 160, is far longer than synergistic effect Evaluation criterion 120.
The Toxicity Determination to citrus red mite for the composition of the present invention：
Test the slide infusion process recommended using FAO (Food and Agriculture Organization of the United Nation) (FAO).20 work inoculated by every piece of slide Female one-tenth mite, sets series concentration value on the basis of 3 pilot studies, and clear water compares, and often processes 3 repetition (totally 60 female one-tenth Mite).Leaching medicine is blotted with blotting paper after 5 seconds immediately, is then placed in 24-26 DEG C of insulating box, checks within 48 hours after medicine dead Rate, calculates corrected mortality.Concentration conversion is become natural logrithm value, corrected mortality is converted into arcsine transformation value, then with anti- Sinusoidal replacement is worth for dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression with DPS Biological Statistic Analysis Software and divides Analysis, draws the toxicity regression linear equation of each medicament.Correction is obtained according to the toxicity regression linear equation of each medicament dead Rate arcsine transformation value (y), draws EC by looking into antilogarithm table50Value, then according to the abundant method of Sun Yun calculate medicament toxicity index and Co-toxicity coefficient (CTC).
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content in mixture for the toxicity index × single dose
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect. Experimental result such as table 14：
Table 14：The toxicity index of each medicament and the co-toxicity coefficient of Tebuconazole dichloro evil bacterium azoles
Table 14 test result indicate that dichloro dislikes bacterium azoles and Tebuconazole in mass ratio 1:50～30:1 compounds, the red spider to oranges and tangerines Spider all has a synergistic effect, and especially proportioning is 1:40～20:When between 1, synergy is more notable.
The Toxicity Determination to oranges and tangerines resin disease (broken skin disease) for the composition of the present invention：
Test adopts mycelial growth rate method, takes the experiment liquid that 5ml prepares to be added to equipped with 45ml hot culture medium (PDA Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, band poured into by each culture dish Medicine culture medium 12ml, each process 4 repetition, horizontal rest, after cooling flat board.With diameter 5mm card punch from culture 4d Cut bacteria cake for examination bacterium edge, with choosing pin, the one side with mycelia is connected on the malicious culture medium of band, all operations are all ultra-clean Workbench carries out sterile working.After process, flat board is placed in 20 DEG C of constant temperature sterile culture case and adopts right-angled intersection after culture 4d Method measures the colony diameter of each process respectively, calculates each mean value processing colony diameter, the average net growth of colony diameter And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((comparison bacterium colony net growth-process bacterium colony net growth)/comparison bacterium colony is net Increment) × 100 concentration conversion is become natural logrithm value, suppress rate conversion arcsin function value, be worth with arcsine letter replacement and be Dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Software, draws recurrence side Journey, then carry out the conversion of anti-natural logrithm and draw EC50.Calculate toxicity index and the co-toxicity coefficient (CTC) of medicament according to the abundant method of Sun Yun.
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content in mixture for the toxicity index × single dose
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect. Experimental result such as table 15；
Table 15：The toxicity index of each medicament and the co-toxicity coefficient of Tebuconazole dichloro evil bacterium azoles
Reagent agent Proportioning EC50(mg/kg) Co-toxicity coefficient
Dichloro dislikes bacterium azoles - 9.88 -
Tebuconazole - 6.21 -
Dichloro dislikes bacterium azoles:Tebuconazole 1：50 4.68 133.67
Dichloro dislikes bacterium azoles:Tebuconazole 1：40 4.12 152.11
Dichloro dislikes bacterium azoles:Tebuconazole 1：30 3.68 170.8
Dichloro dislikes bacterium azoles:Tebuconazole 1：15 3.28 193.83
Dichloro dislikes bacterium azoles:Tebuconazole 1：1 3.63 210.1
Dichloro dislikes bacterium azoles:Tebuconazole 5：1 4.51 199.43
Dichloro dislikes bacterium azoles:Tebuconazole 10：1 4.88 192.14
Dichloro dislikes bacterium azoles:Tebuconazole 15：1 5.19 183.59
Dichloro dislikes bacterium azoles:Tebuconazole 20：1 5.83 164.83
Dichloro dislikes bacterium azoles:Tebuconazole 30：1 6.61 146.67
Table 15 test result indicate that dichloro dislikes bacterium azoles and Tebuconazole composition in mass ratio 1:50～30:1 compounds, to mandarin orange Tangerine fat disease (broken skin disease) all has a synergistic effect, and especially proportioning is 1：40～20：When between 1, synergy is more notable.
The Toxicity Determination to citrus anthracnose for the composition of the present invention：
Test adopts mycelial growth rate method, takes the experiment liquid that 5ml prepares to be added to equipped with 45ml hot culture medium (PDA Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, band poured into by each culture dish Medicine culture medium 12ml, each process 4 repetition, horizontal rest, after cooling flat board.With diameter 5mm card punch from culture 4d Cut bacteria cake for examination bacterium edge, with choosing pin, the one side with mycelia is connected on the malicious culture medium of band, all operations are all ultra-clean Workbench carries out sterile working.After process, flat board is placed in 20 DEG C of constant temperature sterile culture case and adopts right-angled intersection after culture 4d Method measures the colony diameter of each process respectively, calculates each mean value processing colony diameter, the average net growth of colony diameter And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((comparison bacterium colony net growth-process bacterium colony net growth)/comparison bacterium colony is net Increment) × 100 concentration conversion is become natural logrithm value, suppress rate conversion arcsin function value, be worth with arcsine letter replacement and be Dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Software, draws recurrence side Journey, then carry out the conversion of anti-natural logrithm and draw EC50.Calculate toxicity index and the co-toxicity coefficient (CTC) of medicament according to the abundant method of Sun Yun.
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content in mixture for the toxicity index × single dose
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect. Experimental result such as table 16；
Table 16：The toxicity index of each medicament and the co-toxicity coefficient of Tebuconazole dichloro evil bacterium azoles
Table 16 test result indicate that dichloro dislikes bacterium azoles and Tebuconazole composition in mass ratio 1:50～30:1 compounds, to mandarin orange Tangerine anthracnose all has a synergistic effect, and especially proportioning is 1：20～30：When between 1, synergy is more notable.
The Toxicity Determination to citrus bacterial canker disease for the composition of the present invention：
Test adopts mycelial growth rate method, takes the experiment liquid that 5ml prepares to be added to equipped with 45ml hot culture medium (PDA Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, band poured into by each culture dish Medicine culture medium 12ml, each process 4 repetition, horizontal rest, after cooling flat board.With diameter 5mm card punch from culture 4d Cut bacteria cake for examination bacterium edge, with choosing pin, the one side with mycelia is connected on the malicious culture medium of band, all operations are all ultra-clean Workbench carries out sterile working.After process, flat board is placed in 20 DEG C of constant temperature sterile culture case and adopts right-angled intersection after culture 4d Method measures the colony diameter of each process respectively, calculates each mean value processing colony diameter, the average net growth of colony diameter And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((comparison bacterium colony net growth-process bacterium colony net growth)/comparison bacterium colony is net Increment) × 100 concentration conversion is become natural logrithm value, suppress rate conversion arcsin function value, be worth with arcsine letter replacement and be Dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Software, draws recurrence side Journey, then carry out the conversion of anti-natural logrithm and draw EC50.Calculate toxicity index and the co-toxicity coefficient (CTC) of medicament according to the abundant method of Sun Yun.
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content in mixture for the toxicity index × single dose
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect. Experimental result such as table 17；
Table 17：The toxicity index of each medicament and the co-toxicity coefficient of Tebuconazole dichloro evil bacterium azoles
Table 17 test result indicate that dichloro dislikes bacterium azoles and Tebuconazole composition in mass ratio 1:50～30:1 compounds, to mandarin orange Tangerine canker all has a synergistic effect, and especially proportioning is 1：20～30：When between 1, synergy is more notable.
The Toxicity Determination to oranges and tangerines downy mildew for the composition of the present invention：
Test adopts mycelial growth rate method, takes the experiment liquid that 5ml prepares to be added to equipped with 45ml hot culture medium (PDA Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, band poured into by each culture dish Medicine culture medium 12ml, each process 4 repetition, horizontal rest, after cooling flat board.With diameter 5mm card punch from culture 4d Cut bacteria cake for examination bacterium edge, with choosing pin, the one side with mycelia is connected on the malicious culture medium of band, all operations are all ultra-clean Workbench carries out sterile working.After process, flat board is placed in 20 DEG C of constant temperature sterile culture case and adopts right-angled intersection after culture 4d Method measures the colony diameter of each process respectively, calculates each mean value processing colony diameter, the average net growth of colony diameter And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((comparison bacterium colony net growth-process bacterium colony net growth)/comparison bacterium colony is net Increment) × 100 concentration conversion is become natural logrithm value, suppress rate conversion arcsin function value, be worth with arcsine letter replacement and be Dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Software, draws recurrence side Journey, then carry out the conversion of anti-natural logrithm and draw EC50.Calculate toxicity index and the co-toxicity coefficient (CTC) of medicament according to the abundant method of Sun Yun.
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content in mixture for the toxicity index × single dose
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect. Experimental result such as table 18；
Table 18：The toxicity index of each medicament and the co-toxicity coefficient of Tebuconazole dichloro evil bacterium azoles
Reagent agent Proportioning EC50(mg/kg) Co-toxicity coefficient
Dichloro dislikes bacterium azoles -- 6.63 -
Tebuconazole -- 4.38 -
Dichloro dislikes bacterium azoles:Tebuconazole 1:50 3.2 137.79
Dichloro dislikes bacterium azoles:Tebuconazole 1:40 3.01 146.73
Dichloro dislikes bacterium azoles:Tebuconazole 1:30 2.54 174.35
Dichloro dislikes bacterium azoles:Tebuconazole 1:20 3.28 187.06
Dichloro dislikes bacterium azoles:Tebuconazole 1:15 2.26 198.01
Dichloro dislikes bacterium azoles:Tebuconazole 1:10 2.12 213.18
Dichloro dislikes bacterium azoles:Tebuconazole 1:1 2.46 214.43
Dichloro dislikes bacterium azoles:Tebuconazole 5:1 3.08 198.28
Dichloro dislikes bacterium azoles:Tebuconazole 10:1 3.46 183.07
Dichloro dislikes bacterium azoles:Tebuconazole 15:1 3.84 167.29
Dichloro dislikes bacterium azoles:Tebuconazole 20:1 4.02 160.99
Dichloro dislikes bacterium azoles:Tebuconazole 30:1 4.48 145.58
Table 18 test result indicate that dichloro dislikes bacterium azoles and Tebuconazole composition in mass ratio 1:50～30:1 compounds, to mandarin orange Tangerine downy mildew all has a synergistic effect, and especially proportioning is 1：30～30：When between 1, synergy is more notable.
The Toxicity Determination to oranges and tangerines gray mold for the composition of the present invention：
Test adopts mycelial growth rate method, takes the experiment liquid that 5ml prepares to be added to equipped with 45ml hot culture medium (PDA Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, band poured into by each culture dish Medicine culture medium 12ml, each process 4 repetition, horizontal rest, after cooling flat board.With diameter 5mm card punch from culture 4d Cut bacteria cake for examination bacterium edge, with choosing pin, the one side with mycelia is connected on the malicious culture medium of band, all operations are all ultra-clean Workbench carries out sterile working.After process, flat board is placed in 20 DEG C of constant temperature sterile culture case and adopts right-angled intersection after culture 4d Method measures the colony diameter of each process respectively, calculates each mean value processing colony diameter, the average net growth of colony diameter And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((comparison bacterium colony net growth-process bacterium colony net growth)/comparison bacterium colony is net Increment) × 100 concentration conversion is become natural logrithm value, suppress rate conversion arcsin function value, be worth with arcsine letter replacement and be Dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Software, draws recurrence side Journey, then carry out the conversion of anti-natural logrithm and draw EC50.Calculate toxicity index and the co-toxicity coefficient (CTC) of medicament according to the abundant method of Sun Yun.
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content in mixture for the toxicity index × single dose
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect. Experimental result such as table 19；
Table 19：The toxicity index of each medicament and the co-toxicity coefficient of Tebuconazole dichloro evil bacterium azoles
Reagent agent Proportioning EC50(mg/kg) Co-toxicity coefficient
Dichloro dislikes bacterium azoles -- 6.98 -
Tebuconazole -- 4.76 -
Dichloro dislikes bacterium azoles:Tebuconazole 1:50 3.61 132.68
Dichloro dislikes bacterium azoles:Tebuconazole 1:40 3.21 149.45
Dichloro dislikes bacterium azoles:Tebuconazole 1:30 2.78 173
Dichloro dislikes bacterium azoles:Tebuconazole 1:20 2.54 190.28
Dichloro dislikes bacterium azoles:Tebuconazole 1:15 2.38 204.06
Dichloro dislikes bacterium azoles:Tebuconazole 1:10 2.28 214.99
Dichloro dislikes bacterium azoles:Tebuconazole 1:1 2.59 216.58
Dichloro dislikes bacterium azoles:Tebuconazole 5:1 3.01 215.17
Dichloro dislikes bacterium azoles:Tebuconazole 10:1 3.52 190.23
Dichloro dislikes bacterium azoles:Tebuconazole 15:1 3.84 176.62
Dichloro dislikes bacterium azoles:Tebuconazole 20:1 4.01 170.28
Dichloro dislikes bacterium azoles:Tebuconazole 30:1 4.48 152.42
Table 19 test result indicate that dichloro dislikes bacterium azoles and Tebuconazole composition in mass ratio 1:50～30:1 compounds, to mandarin orange Tangerine gray mold all has a synergistic effect, and especially proportioning is 1：40～30：When between 1, synergy is more notable.
The Toxicity Determination to oranges and tangerines blister mite for the composition of the present invention：
Test the slide infusion process recommended using FAO (Food and Agriculture Organization of the United Nation) (FAO).20 work inoculated by every piece of slide Female one-tenth mite, sets series concentration value on the basis of 3 pilot studies, and clear water compares, and often processes 3 repetition (totally 60 female one-tenth Mite).Leaching medicine is blotted with blotting paper after 5 seconds immediately, is then placed in 24-26 DEG C of insulating box, checks within 48 hours after medicine dead Rate, calculates corrected mortality.Concentration conversion is become natural logrithm value, corrected mortality is converted into arcsine transformation value, then with anti- Sinusoidal replacement is worth for dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression with DPS Biological Statistic Analysis Software and divides Analysis, draws the toxicity regression linear equation of each medicament.Correction is obtained according to the toxicity regression linear equation of each medicament dead Rate arcsine transformation value (y), draws EC by looking into antilogarithm table50Value, then according to the abundant method of Sun Yun calculate medicament toxicity index and Co-toxicity coefficient (CTC).
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content in mixture for the toxicity index × single dose
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect. Experimental result such as table 20
Table 20：The toxicity index of each medicament and the co-toxicity coefficient of Tebuconazole dichloro evil bacterium azoles
Reagent agent Proportioning EC50(mg/kg) Co-toxicity coefficient
Dichloro dislikes bacterium azoles -- 8.03 -
Tebuconazole -- 5.72 -
Dichloro dislikes bacterium azoles：Tebuconazole 1：50 4.46 147.04
Dichloro dislikes bacterium azoles：Tebuconazole 1：40 4.12 159.35
Dichloro dislikes bacterium azoles：Tebuconazole 1：30 3.84 171.26
Dichloro dislikes bacterium azoles：Tebuconazole 1：15 3.52 188.08
Dichloro dislikes bacterium azoles：Tebuconazole 1：1 3.42 214.4
Dichloro dislikes bacterium azoles：Tebuconazole 5：1 3.61 221.25
Dichloro dislikes bacterium azoles：Tebuconazole 10：1 3.95 206.39
Dichloro dislikes bacterium azoles：Tebuconazole 15：1 4.31 190.63
Dichloro dislikes bacterium azoles：Tebuconazole 20：1 4.89 168.71
Dichloro dislikes bacterium azoles：Tebuconazole 30：1 5.21 159.02
Table 20 test result indicate that dichloro dislikes bacterium azoles and Tebuconazole in mass ratio 1:50～30:1 compounds, and become rusty wall to oranges and tangerines Lice all has a synergistic effect, and especially proportioning is 1:40～30:When between 1, synergy is more notable.
The Toxicity Determination to orange caterpillar for the composition of the present invention：
Test the slide infusion process recommended using FAO (Food and Agriculture Organization of the United Nation) (FAO).20 work inoculated by every piece of slide Female one-tenth mite, sets series concentration value on the basis of 3 pilot studies, and clear water compares, and often processes 3 repetition (totally 60 female one-tenth Mite).Leaching medicine is blotted with blotting paper after 5 seconds immediately, is then placed in 24-26 DEG C of insulating box, checks within 48 hours after medicine dead Rate, calculates corrected mortality.Concentration conversion is become natural logrithm value, corrected mortality is converted into arcsine transformation value, then with anti- Sinusoidal replacement is worth for dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression with DPS Biological Statistic Analysis Software and divides Analysis, draws the toxicity regression linear equation of each medicament.Correction is obtained according to the toxicity regression linear equation of each medicament dead Rate arcsine transformation value (y), draws EC by looking into antilogarithm table50Value, then according to the abundant method of Sun Yun calculate medicament toxicity index and Co-toxicity coefficient (CTC).
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content in mixture for the toxicity index × single dose
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect. Experimental result such as table 21：
Table 21：The toxicity index of each medicament and the co-toxicity coefficient of Tebuconazole dichloro evil bacterium azoles
Reagent agent Proportioning EC50(mg/kg) Co-toxicity coefficient
Dichloro dislikes bacterium azoles -- 8.03 -
Tebuconazole -- 5.72 -
Dichloro dislikes bacterium azoles：Tebuconazole 1：50 4.82 132.24
Dichloro dislikes bacterium azoles：Tebuconazole 1：40 4.26 149.81
Dichloro dislikes bacterium azoles：Tebuconazole 1：30 3.51 183.73
Dichloro dislikes bacterium azoles：Tebuconazole 1：15 3.29 196.02
Dichloro dislikes bacterium azoles：Tebuconazole 1：1 3.38 216.9
Dichloro dislikes bacterium azoles：Tebuconazole 5：1 3.71 220.6
Dichloro dislikes bacterium azoles：Tebuconazole 10：1 3.99 210.69
Dichloro dislikes bacterium azoles：Tebuconazole 15：1 4.32 196.6
Dichloro dislikes bacterium azoles：Tebuconazole 20：1 4.78 177.68
Dichloro dislikes bacterium azoles：Tebuconazole 30：1 5.03 170.72
Table 21 test result indicate that dichloro dislikes bacterium azoles and Tebuconazole in mass ratio 1:50～30:1 compounds, to orange caterpillar All there is a synergistic effect, especially proportioning is 1:40～30:When between 1, synergy is more notable.

Claims (9)

1. a kind of composition pesticide disliking bacterium azoles and Tebuconazole containing dichloro, including active component it is characterised in that：Described activity becomes It is divided into dichloro to dislike bacterium azoles and Tebuconazole, be calculated by weight as：
Dichloro dislikes bacterium azoles 1-30 part；
Tebuconazole 1-50 part.
2. according to claim 1 a kind of containing dichloro dislike bacterium azoles and Tebuconazole composition pesticide it is characterised in that：Described It is 1-5 part that dichloro dislikes bacterium azoles, and described Tebuconazole is 1-50 part.
3. according to claim 1 a kind of containing dichloro dislike bacterium azoles and Tebuconazole composition pesticide it is characterised in that：Described It is 1-20 part that dichloro dislikes bacterium azoles, and described Tebuconazole is 1-30 part.
4. according to claim 1 a kind of containing dichloro dislike bacterium azoles and Tebuconazole composition pesticide it is characterised in that：Described It is 1-15 part that dichloro dislikes bacterium azoles, and described Tebuconazole is 1-30 part.
5. according to claim 1 a kind of containing dichloro dislike bacterium azoles and Tebuconazole composition pesticide it is characterised in that：Described It is 1-30 part that dichloro dislikes bacterium azoles, and described Tebuconazole is 1-30 part.
6. a kind of composition pesticide disliking bacterium azoles and Tebuconazole containing dichloro according to any one of claim 1-5, it is special Levy and be：This composition pesticide also includes auxiliary agent, described auxiliary agent include dispersant, emulsifying agent, antifreezing agent, thickener, defoamer, One or more of solvent, wetting agent, disintegrant and filler.
7. according to claim 6 a kind of containing dichloro dislike bacterium azoles and Tebuconazole composition pesticide it is characterised in that：Described Auxiliary agent add parts by weight be：
Dispersant 0.1-15 part；
Emulsifying agent 0.1-12 part；
Antifreezing agent 0.1-4 part；
Thickener 0.1-4 part；
Defoamer 0.1-2 part；
Solvent 25.9-95.8 part；
Wetting agent 0.1-15 part；
Disintegrant 0.1-6 part；
Filler 8-97.5 part.
8. according to claim 6 a kind of containing dichloro dislike bacterium azoles and Tebuconazole composition pesticide it is characterised in that：Described Emulsifying agent is calcium dodecyl benzene sulfonate, alkylpolyoxyethylene, styrylphenol polyoxyethylene ether, polyoxyethylene nonyl phenyl Vinethene, thermally coupled distillation columns, aliphatic acid polyethenoxy base ester, polyoxyethylene aliphatic alcohol ether, dodecyl gather One or more of oxygen ethenesulfonic acid sodium salt and phenethyl phenol polyethenoxy ether；
Described solvent is dimethylbenzene, diesel oil, toluene, cyclohexanol, methyl alcohol, soybean oil, turpentine oil, methyl oleate, solvent naphtha, diformazan One of base formamide, ethanol, N- pyrrolidones, ethyl acetate and water or two or more combinations；
Described wetting agent is lauryl sodium sulfate, calcium dodecyl benzene sulfonate, methyl naphthalene sulfonate formaldehyde condensate, pulls open One of powder, Negel and spaonin powder or two or more combinations；
Described dispersant be sodium lignin sulfonate, calcium lignosulfonate, APES formaldehyde condensation product sodium sulphate, ten Dialkyl benzene sulfonic acids calcium, APES, polycarboxylic acids sodium, aliphatic amine polyoxyethylene ether and glycerin polyoxyethylene ether fat One of acid esters or two or more combinations；
Described antifreezing agent is one of ethylene glycol, propane diols, glycerine and urea or two or more combinations；
Described thickener is one of xanthans, Magnesiumaluminumsilicate, hydroxymethyl cellulose and polyvinyl alcohol or two or more groups Close；
Described defoamer is one of silicone oil, organic silicone and polypropylene glycerol aether or two or more combinations；
Described disintegrant is one of bentonite, ammonium sulfate, sodium acid carbonate, aluminium chloride and succinic acid or two or more groups Close；
Described filler is one of white carbon, kaolin, diatomite, precipitated calcium carbonate and starch or two or more combinations.
9. according to claim 6 a kind of containing dichloro dislike bacterium azoles and Tebuconazole composition pesticide it is characterised in that：Agricultural chemicals The formulation of composition is suspending agent, aqueous emulsion, water dispersible granules, microemulsion or wettable powder.
CN201610793024.6A 2016-08-31 2016-08-31 Pesticide composition containing 2-methylsulfonyl-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and tebuconazole Pending CN106386820A (en)

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