CN1063787C

CN1063787C - Application of candelabrella sp on agriculture

Info

Publication number: CN1063787C
Application number: CN94100221A
Authority: CN
Inventors: 周广泉; 蒋冬荣
Original assignee: GUANGXI PLANT INST
Current assignee: GUANGXI PLANT INST
Priority date: 1994-01-05
Filing date: 1994-01-05
Publication date: 2001-03-28
Anticipated expiration: 2014-01-05
Also published as: CN1105066A

Abstract

The present invention relates to an application of Candelabrella sp in agriculture. A strain is screened out by a soil plate method of a corn flour culture medium, nematodes are added to a culture dish so as to successfully and rapidly complete screening when the air temperature is 30 DEG C, and the strain can be used after three-stage culture. The strain can be cultured by monospore isolation or the alternative use of the corn flour culture medium and a PDA culture medium if the strain degrades. The microbial inoculum can prey on nematodes at the roots of grosvenor momordica fruit, tobacco, nightshades and sugarcanes, and the specially good effect period is one year. The microbial inoculum has the main functions of preying on nematodes, secreting antibiotics, resisting various root diseases, promoting the growth and the development of plants, increasing yield and improving quality.

Description

The application of candelabrella sp on agricultural

The present invention relates to the application of carnivorous fungi microbe on agricultural.

Nematode harms the crops widely, for everybody confirms, calculates according to USDA, near 10% of insect pest summation.Nematode host's specialization is not strong, though imprecisely know that certain nematode can infect all plants, we can say that not having a kind of vascular plant all is immune to all parasitic nematodes, so the harm of nematode is apparent.The twenty's of this century causes the concern of organic sphere, and the forties is produced efficient nematode killing agent, as the furans pellet.Adopt chemical nematode killing agent, people and animals are had in various degree murder by poisoning, polluted environment; It is surplus that host plant has residual hazard to stay, and belongs to the food person and avoided by people; Use for a long time with chemical nematode killing agent furans pellet, nematode develops immunity to drugs, and has to increase dose, and cost is more and more higher, and environmental pollution is on the rise.Annual breeding has 4 to 5 generations or more and nematode is in China south.

For keeping a good ecotope, prevent soil pollution, people bet sight in biological control." a kind of fungi that prevents and treats nematode " according to 1989 1 phases " plant protection " introduction.Paecilomyces lilacinus Paccilomyces lilacinus is the soil facultative saprophyte, after the packing of nematode or ovum contact, stretches into chorion.A series of superstructures have taken place in chorion changed, chorion is broken.If formed larva in the ovum, this fungi is just renderd a service and weakens greatly.The defective of this kind fungi also is: apply undergroundly before broadcasting, crop growth period is just executed and has been produced little effect; Claim according to reports also can only be confined to the potato root knot nematode; Effect and furans pellet are similar.

The objective of the invention is to disclose a kind of, can be used for the fungi candelabrella sp Candelabrella of perennial plant unspent, carnivorous seizure nematode of next year various plants, free from environmental pollution, perennial to the public

The present invention is candelabrella sp Candelabrella sp in the fungi, with pure strain method, is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on October 14th, 1993, is numbered CGMCC No. 0201.Detected the bacterial strain survival on October 18th, 1993 according to depositary institution.

The screening method of this bacterium adopts agar disk method, agar strip coating method, soil plate method respectively, and (this three therapeutic methods of traditional Chinese medicine is seen L, F, Johnson EA Ke Er, soil-borne plant pathogen ecological Studies method, the disease research department is planted by Beijing Agricultural University), directly postmortem method, (this two method is seen 1979 agriculture press to the graceful funnel method of shellfish, the plant research method, Fang Zhongda).These five kinds of methods adopt any, directly from different areas be rich in corruption plant the different plant rhizosphere soils of matter and the nematode of diseased plant on, isolate

This hyphae colorless, conidiophore is elongated, and is upright, colourless, and end is given birth to little storied limb ray, and shape is gained the name like candlestick.The conidium of 2 to 5 cells, colourless long clavate are given birth in the top.Under neutrality or alkaline condition, be down pyriform or ellipse, can produce on the mycelia and have the ring that three microscler cells link to each other and to surround, i.e. the organ of nematode-trapping, nematode touches then cell expansion and vise nematode of its inboard.Its secretory product can be cleared up nematode, i.e. nematode-trapping.This bacterial strain is stablized by force the gesture of catching of nematode.This bacteria growing comfort zone is wide.All can normal growth between 20～30 degree Celsius.The little acid of property happiness.Happiness oxygen is water-fast.

Confirm as genus Mycophyta (Fungi) imperfect fungi (Fungiimperfecfi) according to form of the present invention, Moniliales (Moniliales), many spores of Moniliaceae subfamily (Phragmosporoideae Moniliaceae) candelabrella sp (Candelabre lla sp).

Adopt maize powder medium, (1000 milliliters in agar 20 gram Semen Maydis powder 20 gram water).Place 20 to 30 degree Celsius the substratum ware is housed, potential of hydrogen 4.5-9 can filter out the bacterial classification that adapts under the hot conditions.Insert nematode in culture dish, assorted bacterium destroys.After bacterial classification grows, can be used as original seed (being the one-level kind), treat that first class inoculum grows up to after, bacterial classification is moved into secondary kind substratum.Under 20-30 Celsius ℃ condition, need 7-14 days.

The composition of secondary kind substratum is: boil soft Chinese sorghum and account for 90% of volume, husk accounts for volume 10%, water a little, can make that substratum is moistening to get final product.Train and move into three grades of kind substratum after 10-14 days, temperature is the same.

The composition of three grades of kind substratum is: boil soft Chinese sorghum and account for 30% of volume, wheat bran accounts for 30% of volume, and husk accounts for 40%, water a little, (between 1650 to 1760 milliliters).Room temperature 20-30 degree was cultivated 10-14 days.

Used this bacterial classification previous month, well by 8 to 10 jin of calculations of every mu of consumption bacterial manure.With the isopyknic organic fertilizer that becomes thoroughly decomposed of bacterial classification, mix thoroughly.The loam cake plastics film, macerate system, the time spent executes around crop root getting final product to the fertilizer that is mixed with bacterial classification.

As find the bacterial strain degeneration, but rejuvenation bacterial strain.Under powerful microscope, the monospore that chooses is peeled off at aseptic condition with needle point.On maize powder medium, cultivate again.Culture temperature is a 20-28 degree Celsius, a rejuvenation generation.Perhaps maize powder medium and PDA substratum substitute use, have substituted once just.

Description of drawings:

Fig. 1 candelabrella sp conidium branch synoptic diagram

Fig. 2 candelabrella sp capture ring synoptic diagram

Fig. 3 candelabrella sp is the conidium synoptic diagram under alkaline condition

The carnivorous fungi of the present invention is take the parasitic nematode of control root system of plant as object, to plant The son for the sowing material the host, catch the integrated control effect of nematode greater than 70%, to take trophosome as The host of sowing material, integrated control effect reaches 60%, and the lasting period is 1 year. Also to tobacco, The bacterial wilt of tomato and the wilt disease of watermelon have the anti-effect of holding concurrently. Especially to continuous cropping ground tobacco bacterial wilt Preventive effect can reach 60%. The secretion of this bacterium has the effect of stimulating growth to host plant, uses This bacterium is free from environmental pollution, to people and animals' nonhazardous.

Table one Momordica grosvenori canopy nematode-trapping effect table

Process	The diseased plant number	The soil line borer population	Preventive effect	Sick level strain number^*				Disease index	Diseased plant rate
				Sick level strain number^*						The I level	The II level	The III level	The IV level
				Use microbial inoculum	0	78	67．2％			The I level	The II level	The III level	The IV level	6	0	0	0	0	0
Furadan	3	189	20．6％	Use microbial inoculum	0	78	67．2％	3	2	1	0	22．2	50．0％	6	0	0	0	0	0
Furadan	3	189	20．6％	C．K	5	238	0．0	3	2	1	0	22．2	50．0％	1	1	2	2	61．1	83．3％

^*I level: do not have nematode gall on the root; II level: 1～3 of eelworm goitre; The III level: the eelworm goitre is more than 4; The IV level: nematode gall is many and big.

The preventive effect of table two watermelon nematodiasis, wilt disease and yield increase effect table thereof

Process	The strain number	The wilt disease death rate	First melon gross output kg	Total number	Average row	Average kg	The nematode-trapping preventive effect		Rate of growth %
							The nematode-trapping preventive effect			April preventive effect	Month preventive effect
							Use microbial inoculum	534		April preventive effect	Month preventive effect	39．3	603	93	15．5	12．97	65．3％	62．3％	70．3
Execute Furadan	89	67．4	42．5	7	7．0	12．14	Use microbial inoculum	534	41．6％	10．6％	28．0	39．3	603	93	15．5	12．97	65．3％	62．3％	70．3
Execute Furadan	89	67．4	42．5	7	7．0	12．14	Contrast	178	41．6％	10．6％	28．0	63．5	118	19	8．5	12．42	0．0	0．0	-

Table three microbial inoculum contrasts average 15.3 59.1 0.0 low peak period-2.8～7.5 7.5, peak period 6.1～33.3 56.7～61.4 0.0～48.2 0.0 average 2.0 32.8 0.0 overall averages 8.7 45.9 0.0 to the prevention effect nematode of Momordica grosvenori planting site soil middle reaches off-line worm from numerous this bacterium of phase Furadan preventive effect % preventive effect %

Annotate: data are inspection in every month statistics once in the table.

The average stem of special efficacy phase effect process of table four sugarcane nematode germ agent control is thick, (cm) average plant height, (cm) average stem is heavy, (kg) amount to are, (kg) volume increase, (%) inferior annual rate fruit 2.49 182.00 0.71 237.61 8.09 contrasts 2.39 188.83 0.69 219.82/

Embodiment adopts maize powder medium, screens bacterial strain with soil plate method, and effect is best, the bacterial strain that filters out, be original seed, also can use, the one-level kind is inoculated into secondary kind substratum between sterilization as the one-level kind, under 25-30 ℃ of condition, cultivated 10-14 days, and can produce three grades of kinds thus as the inoculation material of three grades of kinds, still grew under these conditions 10-14 days, and can use.

Be applied to one mu of tobacco, with 10 jin of bacterial manure amounts.Mix, macerate ripe with isopyknic fertilizer that becomes thoroughly decomposed, cover plastics, parked 20-30 days.Get final product around imposing on the tobacco root.

Claims

1, a kind of candelabrella sp Candelabrella sp fungal bacterial strain is characterized in that with pure strain method, is deposited in Chinese microorganism center, is numbered the bacterial strain of CGMCC NO:0201 on October 14th, 1993.

2, fungal bacterial strain according to claim 1 is characterized in that, to the rejuvenation of degenerative strain, is to peel off with needle point under powerful microscope, cultivates in corn culture medium, or cultivates it with maize powder medium and PD, A and more pass use.

3, the application of candelabrella sp Candelabrella sp is characterized in that, microbial inoculum and the equal-volume organic fertilizer that becomes thoroughly decomposed is mixed thoroughly, imposes on around Grosvenor Momordica, tobacco, tomato, celery, the watermelon root.