CN106376601A - Spreading oil suspension, and application thereof in control of insect diseases of crops - Google Patents
Spreading oil suspension, and application thereof in control of insect diseases of crops Download PDFInfo
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- CN106376601A CN106376601A CN201610794422.XA CN201610794422A CN106376601A CN 106376601 A CN106376601 A CN 106376601A CN 201610794422 A CN201610794422 A CN 201610794422A CN 106376601 A CN106376601 A CN 106376601A
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- suspending agent
- beauveria bassiana
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The invention provides a Beauveria bassiana-containing spreading oil suspension. The spreading oil suspension comprises, by weight, 0.1-60% of an effective component comprising Beauveria bassiana, 1.0-20% of a surfactant, 2.0-20% of a film forming agent, and the balance of an oily disperse medium. The Beauveria bassiana spreading oil suspension has a substantial control effect on insect diseases, and the control efficiency can reach 84-85%. Compared with traditional spreading oil suspensions, the Beauveria bassiana spreading oil suspension has the advantages of good effects, and good spreading performance on the liquid level.
Description
Technical field
The present invention relates to biological pesticide technical field, more particularly, to one kind open up film oil-suspending agent and its in preventing and treating crops elder brother
The application of pests aspect.
Background technology
Film-spreading oil solution is to impose on the oil preparation that the water surface forms thin film, has prevention effect outstanding feature, and dart transfer is prevented and treated
Expense is low, and compared with other pesticide, consumption and cost are all relatively low.And in use, easy and simple to handle, without plant protection device
Tool, directly instills medicine in water, improves 20 times of work efficiency than conventional dispenser, greatly mitigate dispenser labor intensity, be conducive to labour to turn
Change, change existing traditional heavy application method.In addition no matter the impact of this dosage form not climate, rain or cloudy in Control stage
It all can directly drip medicine.This dosage form belongs to low toxicity dosage form, due to special insecticide-applying way so as to not produce medicine in use
Liquid drift, free from environmental pollution, do not produce poisoning, little to natural enemy lethality, to person poultry safety.
And the dosage form product of film-spreading oil solution does not need to be watered dilution, directly spread and can sprawl rapidly after dropping in the crop water surface
Open, form one layer of uniform medicine film, simultaneously in the presence of auxiliary agent, medicine film can be sprawled upwards along crop stem, concentrates on water
Plant stalk base portion, thus realize effective preventing and treating of the aquatic crops disease pest and weed that conventional insecticide-applying way is difficult to.
However, the species of existing film-spreading oil solution is not good enough in water surface spreading property, the preventing and treating to product of the medicine film performance of formation
Effect has larger fluctuating.
Content of the invention
In view of this, present invention aims to the deficiencies in the prior art, and provide and a kind of contain beauveria bassiana
Exhibition film oil-suspending agent.
In order to realize foregoing invention purpose, the present invention provides technical scheme below：
The invention provides a kind of exhibition film oil-suspending agent, including the component of following weight/mass percentage composition：Effective ingredient 0.1～
60%, described effective ingredient includes beauveria bassiana；Surfactant 1.0～20%；Film former 2.0～20%；Surplus
Oiliness disperse medium.
Preferably, spore content of living in described beauveria bassiana is 100～30,000,000,000/gram.
Preferably, described effective ingredient also includes organic insecticide, and described organic insecticide is methylamino Avermectin
Element, MTI-446, Diacloden, avilamycin, thiacloprid, pleocidin, flonicamid, d ichlorbenzuron, Rynaxypyr, deinsectization
One or more of urea, effective cypermethrin, decises and indoxacarb.
Preferably, described beauveria bassiana and the weight ratio of organic insecticide are (1～45)：(1～55).
Preferably, described film former includes one of Lauryl Alcohol ester, octadecanol, propylene glycol phenylate and monobutyl ether acetate
Or it is several.
Preferably, weight/mass percentage composition in exhibition film oil-suspending agent for the described film former is 5～15%.
Preferably, described surfactant includes one of nonyl phenol oxygen vinyl Ether and methyl oleate or two kinds.
Preferably, the surfactant including 5～18%.
Preferably, described oiliness disperse medium is one of white oil, dimethylbenzene, solvent naphtha, methyl naphthalene and ethanol or several
Kind.
Further, the invention provides described opens up film oil-suspending agent answering in terms of preventing and treating crops insect pest
With.
A kind of exhibition film oil-suspending agent containing beauveria bassiana that the present invention provides, including the group of following weight percentage
Point：Effective ingredient 0.1～60%, described effective ingredient includes beauveria bassiana；Surfactant 1.0～20%；Film former 2.0
～20%；Balance of oiliness disperse medium.The exhibition film oil-suspending agent of the beauveria bassiana that the present invention provides is prevented to insect pest
Control effect is significant, prevention effect is up to 84～85%.The exhibition film oil-suspending agent of the beauveria bassiana that the present invention provides compares tradition
Exhibition film oil-suspending agent is respond well, and exhibition film oil-suspending agent is in the spread excellent performance of liquid level.
Biological deposits prove
Beauveria bassiana (Beauveria bassiana), was deposited in Chinese microorganism strain and protects on 08 13rd, 2013
Hide administration committee's common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the micro- life of the Chinese Academy of Sciences
Thing institute, it is CGMCC NO.7997 or CGMCC NO.7998 that biological deposits are numbered.
Specific embodiment
The invention provides a kind of exhibition film oil-suspending agent, including the component of following weight/mass percentage composition：Effective ingredient 0.1～
60%, described effective ingredient includes beauveria bassiana；Surfactant 1.0～20%；Film former 2.0～20%；Surplus
Oiliness disperse medium.
The mass content of effective ingredient described in the present invention is preferably 0.1～60%, more preferably 10～45%,
It is preferably 18～35%.Heretofore described effective ingredient includes beauveria bassiana, spore of living in described beauveria bassiana
Content is preferably 100～30,000,000,000/gram, more preferably 150～25,000,000,000/gram, most preferably 20,000,000,000/gram.
In the present invention, described beauveria bassiana be preferably selected from biological deposits and number be CGMCC NO.7997 and/or
The strain activation and culture of CGMCC NO.7998 obtains.
In the present invention, the preparation method of described beauveria bassiana comprises the following steps：
1) by beauveria bassiana actication of culture；
2) beauveria bassiana after described activation is inoculated in beauveria bassiana fluid medium and carries out liquid culture, training
Support 30～50h and stop culture, obtain beauveria bassiana culture fluid；
3) by described step 2) the beauveria bassiana culture fluid that obtains is inoculated in beauveria bassiana solid medium, carries out
Solid fermentation, obtains beauveria bassiana.
In the present invention, the activation of beauveria bassiana strain is that the beauveria bassiana strain of preservation is inoculated in equipped with nutrition fine jade
In the eggplant type bottle of fat culture medium, 25-28 DEG C of culture 8～12h carries out activation culture.
In the present invention, described activation after beauveria bassiana be inoculated in fluid medium inoculum concentration be preferably 20～
30%, more preferably 23～27%, most preferably 25%.
In the present invention, described fluid medium preferably includes following components：Analysis for soybean powder 30～50g/L, white sugar 20～
40g/L, flour 0.01～0.05g/L, dehydrated potato powder 0.01～0.05g/L, dipotassium hydrogen phosphate 0.0002g/L；More preferably：Yellow
Semen Glycines powder 35～45g/L, white sugar 25～35g/L, flour 0.02～0.04g/L, dehydrated potato powder 0.02～0.04g/L, phosphoric acid hydrogen
Dipotassium 0.0002g/L；Most preferably：Analysis for soybean powder 40g/L, white sugar 30g/L, flour 0.03g/L, dehydrated potato powder 0.03g/L, phosphorus
Sour hydrogen dipotassium 0.0002g/L.
In the present invention, described liquid culture preferably uses after 120～130 DEG C of sterilizing 30～45min.The present invention is to training
The equipment of foster base sterilizing does not have special restriction, using sterilizing installation well known to those skilled in the art, such as high steam
Autoclave.
The present invention does not have special restriction to described culture device, using Zymolysis Equipment well known to those skilled in the art is
Can, such as fermentation tank.
In the present invention, the temperature of described beauveria bassiana liquid culture is preferably 25～30 DEG C, and more preferably 26～29
DEG C, most preferably 28 DEG C；The pH value of described beauveria bassiana liquid culture is preferably 6.6～7.2, more preferably 6.8～7.0,
Most preferably 6.9；Pressure inside the tank is preferably 0.05～0.1MPa, more preferably 0.07～0.08MPa；Described liquid culture logical
Tolerance is preferably 0.8～1.2V/V min, more preferably 1V/V min；The time of described liquid culture is preferably 30～50h,
More preferably 35～45h, most preferably 40h.
After completing described beauveria bassiana liquid culture, the beauveria bassiana obtaining culture fluid is inoculated in solid by the present invention
In culture medium, carry out solid fermentation, obtain solid beauveria bassiana.In the present invention, described beauveria bassiana fermentation liquid inoculation
Inoculum concentration in solid medium is preferably 20～30%, more preferably 23～27%, most preferably 25%.
In the present invention, described solid medium preferably includes the component of following weight portion：50～90 parts of Testa Tritici, Semen Maydis powder
10～30 parts, 5～15 parts of rice husk；More preferably 60～80 parts of Testa Tritici, 15～25 parts of Semen Maydis powder, 7～13 parts of rice husk；Most preferably
70 parts of Testa Tritici, 20 parts of Semen Maydis powder, 10 parts of rice husk.The present invention adds distilled water in described beauveria bassiana solid medium, makes
Solid medium water content is preferably 45%～55%, more preferably 47%～52%, most preferably 50%, then carries out solid
Fermentation.
In the present invention, the culture medium thickness of described solid culture are preferably 1～6cm, more preferably 2～4cm, most preferably
For 3cm.
In the present invention, the temperature of described beauveria bassiana solid fermentation is preferably 24～30 DEG C, and more preferably 26～29
DEG C, most preferably 28 DEG C；The humidity of described beauveria bassiana solid fermentation preferably 40%～95%, more preferably 55%～
75%, most preferably 65%；The time of described beauveria bassiana solid fermentation is preferably 48～96h, more preferably 60～84h,
Most preferably 72h.
After described solid fermentation, preferred pair of the present invention obtains being collected of spore powder, obtains beauveria bassiana spore.?
In the present invention, the collection of described spore powder is specifically carried out using the instrument of microorganism solid fermentation fungal spore separation equipment.Institute
The instrument stating microorganism solid fermentation fungal spore separation equipment is purchased from Jiangxi Tianren Ecology Co., Ltd..
In the present invention, described effective ingredient preferably also includes organic insecticide；Specifically described organic insecticide is preferred
For Affirm (Merck Co.), MTI-446, Diacloden, avilamycin, thiacloprid, pleocidin, flonicamid, d ichlorbenzuron, chlorine
One or more of worm Benzoylamide, diflubenzuron, effective cypermethrin, decises and indoxacarb.The present invention has to above-mentioned
The source of machine insecticide is not particularly limited, using commercial goods.
In the present invention, when described effective ingredient includes beauveria bassiana and organic insecticide simultaneously, beauveria bassiana
It is preferably (1～45) with the mass ratio of organic insecticide：(1～55), more preferably (4～40)：(2～50), most preferably (10
～30)：(10～35).
The exhibition film oil-suspending agent that the present invention provides includes the surfactant that mass content is 1.0～20%, preferably 5～
18%, more preferably 10～16%.In the present invention, described surfactant is not particularly limited, using this area skill
Commercial goods known to art personnel.It is preferably specifically one of nonyl phenol oxygen vinyl Ether and methyl oleate or two
Kind, more preferably nonyl phenol oxygen vinyl Ether and methyl oleate.When described surfactant is above two reagent, described nonyl
The mass ratio of phenol oxygen vinyl Ether and methyl oleate is preferably (2～10)：(2～10), more preferably (4～8)：(4～8), optimum
Elect (5～7) as：(5～7).In the present invention, described SURFACTANT ADSORPTION, in pesticide microparticle surfaces, forms different dispersions
System, plays emulsifying, moistening, solubilising, froth breaking, stable effect.During Pesticide use, surfactant can improve medicine
Distribution on plant leaf surface or controlling object surface for the liquid, attachment, infiltration etc., improve the effective transfer of pesticide dosage, directly or
Indirectly improve the effective rate of utilization of pesticide.
The exhibition film oil-suspending agent that the present invention provides includes the film former that mass content is 2.0～20%, preferably 5～
15%, more preferably 8～12%.In the present invention, the source of described film former is not particularly limited, using art technology
The conventional commercial goods selected of personnel.It is preferably specifically Lauryl Alcohol ester, octadecanol, propylene glycol phenylate and butyl ether vinegar
One or more of acid esters, more preferably 2～3 kinds, most preferably 4 kinds.When described film former is 2～3 kinds of mentioned reagent
When, can arbitrary proportion mixing.When described film former is 4 kinds of mentioned reagent, described Lauryl Alcohol ester and octadecanol, propylene glycol
Phenylate, the mass ratio of monobutyl ether acetate are preferably (1～7)：(1～9)：(0.5～9)：(0.5～6), more preferably (2～6)：
(2～8)：(1～8)：((1～5) elects (3～5) most as：(4～6)：(4～5)：(2～4).In the present invention, described one-tenth
Film oil-suspending agent Plastic Flow and elastic deformation are opened up in the promotion that act as of membrane, and exhibition film oil-suspending agent can be made to have more preferable paving
Malleability energy.
The exhibition film oil-suspending agent of the beauveria bassiana that the present invention provides includes the oiliness disperse medium of surplus.In the present invention
In, the source of described oiliness disperse medium is not particularly limited, using conventional commercial commodity well known to those skilled in the art
?.It is preferably specifically one or more of white oil, dimethylbenzene, solvent naphtha, methyl naphthalene and ethanol.Oiliness disperse medium
Can be specifically arbitrarily several in mentioned reagent, can arbitrary proportion mixing.In the present invention, described oiliness disperse medium can
Effective ingredient is uniformly dispersed, makes that exhibition film oil-suspending agent is homogeneous, dispersive property is good.
The preparation method of the exhibition film oil-suspending agent to described beauveria bassiana for the present invention does not have special restriction, using ability
The preparation method of the exhibition film oil-suspending agent known to field technique personnel.Specifically can be by described effective ingredient, surface activity
Prepare after agent, film former and the mixing of oiliness disperse medium.
Present invention also offers beauveria bassiana opens up application in terms of preventing and treating crops insect pest for the film oil-suspending agent.Tool
Body, applicable crop specie includes cereal crops, industrial crops, vegetable crop, orchard crop, forage crop etc..This
The described beauveria bassiana of invention opens up lepidoptera pest and the coleopteran pest that film oil-suspending agent can be prevented and treated in various crop, such as
Pieris rapae, diamondback moth, bollworm, Pyrausta nubilalis (Hubern)., chafer, trialeurodes vaporariorum, aphid, greenery Cicadae, white backed planthopper etc..
The application method of the exhibition film oil-suspending agent to described beauveria bassiana for the present invention is not particularly limited, using this area
The application method of the exhibition film oil-suspending agent known to technical staff.
Below in conjunction with the embodiment in the present invention, the technical scheme in the present invention is clearly and completely described.Aobvious
So, described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on the reality in the present invention
Apply example, the every other embodiment that those of ordinary skill in the art are obtained under the premise of not making creative work, all belong to
In the scope of protection of the invention.
Following examples active constituent content is all added as after hundred kilograms of calculating of folding, and wherein beauveria bassiana is as no referred in particular to
Go out spore content, spore content is 100～30,000,000,000/gram.
Embodiment 1
The preparation of beauveria bassiana:The beauveria bassiana strain of preservation is inoculated in the eggplant type equipped with nutrient agar
In bottle, 26 DEG C of culture 9h carry out activation culture；Beauveria bassiana after activation culture is inoculated in 121 DEG C with 25% inoculum concentration and goes out
In fluid medium after bacterium 35min, fermentation tank carries out liquid culture, specific condition of culture is：Temperature is 28 DEG C；pH
It is worth for 6.9；Pressure inside the tank is 0.08MPa, and culture ventilation is 1V/V min；Stop culture after culture 40h, obtain ball spore white
Stiff bacteria culture fluid；The ball obtaining spore Bombyx Batryticatus culture fluid is inoculated in solid medium with 25% inoculum concentration, in solid culture
Add distilled water in base, make solid medium water content be 50%, then carry out solid fermentation, fermentation condition is：Fermentation temperature
For 26 DEG C, fermentation humidity is 65%, and fermentation time is 72h；Bacterium material after solid fermentation is adopted microbe in solid state to cultivate funguses
Spore separation equipment collects beauveria bassiana spore, and the beauveria bassiana obtaining effective spore amount is 100～30,000,000,000/gram.Will
The spore collected examines under a microscope spore shape, to be defined as beauveria bassiana spore.
Embodiment 2
By weight percentage, the beauveria bassiana 30% prepared by embodiment 1, nonyl phenol oxygen vinyl Ether 7.5%, Oleic acid
Methyl ester 7.5%, Lauryl Alcohol ester 2%, octadecanol 8%, propylene glycol phenylate 7%, monobutyl ether acetate 1%, solvent naphtha complements to
100%.
Preparation technology：In the mixing kettle of belt stirrer, first by beauveria bassiana, nonyl phenol oxygen vinyl Ether, methyl oleate,
Lauryl Alcohol ester, octadecanol, propylene glycol phenylate, monobutyl ether acetate, solvent naphtha, after being thoroughly mixed, then pump in sand mill
Sand milling, after sand milling to granular size D90≤5 μm, obtains final product the exhibition film oil-suspending agent of beauveria bassiana containing sorosphere.
According to preparation technology same as Example 2, prepare following examples.
Embodiment 3
By weight percentage, the beauveria bassiana 10% prepared by embodiment 1, Affirm (Merck Co.) 2%, nonyl phenol
Oxygen vinyl Ether 7.5%, methyl oleate 8%, Lauryl Alcohol ester 6%, octadecanol 2%, propylene glycol phenylate 1%, monobutyl ether acetate
5%, white oil 20%, ethanol complements to 100%；Obtain the oil of the exhibition film containing Affirm (Merck Co.) and beauveria bassiana to suspend
Agent.
Embodiment 4
By weight percentage, the beauveria bassiana 30% prepared by embodiment 1, MTI-446 20%, nonyl phenol oxygen ethylene
Ether 5%, methyl oleate 10%, Lauryl Alcohol ester 3%, octadecanol 4%, propylene glycol phenylate 8%, monobutyl ether acetate 3%, ethanol
8%, methyl naphthalene complements to 100%.Obtain the exhibition film oil-suspending agent containing MTI-446 and beauveria bassiana..
Embodiment 5
By weight percentage, the beauveria bassiana 20% prepared by embodiment 1, Diacloden 14%, nonyl phenol oxygen ethylene
Ether 89%, methyl oleate 10%, Lauryl Alcohol ester 5%, white oil complements to 100%, obtains containing Diacloden and beauveria bassiana
Exhibition film oil-suspending agent.
Embodiment 6
By weight percentage, the beauveria bassiana 30% prepared by embodiment 1, avilamycin 6%, nonyl phenol oxygen ethylene
Ether 5%, methyl oleate 4.5%, octadecanol 3%, propylene glycol phenylate 4%, solvent naphtha complements to 100%.Obtain containing Avermectin
Element and the exhibition film oil-suspending agent of beauveria bassiana.
Embodiment 7
By weight percentage, the beauveria bassiana 40% prepared by embodiment 1, thiacloprid 15%, nonyl phenol oxygen ethylene
Ether 10%, methyl oleate 7%, monobutyl ether acetate 3%, Lauryl Alcohol ester 6%, white oil complements to 100%, obtains containing thiacloprid
Exhibition film oil-suspending agent with beauveria bassiana.
Embodiment 8
By weight percentage, the beauveria bassiana 6% prepared by embodiment 1, flonicamid 20%, nonyl phenol oxygen second
Alkene ether 4%, methyl oleate 10%, Lauryl Alcohol ester 4%, octadecanol 4%, propylene glycol phenylate 5%, monobutyl ether acetate 3%, ethanol
Complement to 100%, obtain the exhibition film oil-suspending agent containing flonicamid and beauveria bassiana.
Embodiment 9
By weight percentage, the beauveria bassiana 4% prepared by embodiment 1, chloromethiuron 50%, nonyl phenol oxygen vinyl Ether
6%, methyl oleate 6%, Lauryl Alcohol ester 2%, octadecanol 4%, propylene glycol phenylate 3%, monobutyl ether acetate 2%, dimethylbenzene is mended
Foot, to 100%, obtains the exhibition film oil-suspending agent containing d ichlorbenzuron and beauveria bassiana.
Embodiment 10
By weight percentage, the beauveria bassiana 15% prepared by embodiment 1, Rynaxypyr 25%, nonyl phenol
Oxygen vinyl Ether 6%, methyl oleate 10%, Lauryl Alcohol ester 5%, octadecanol 6%, propylene glycol phenylate 4%, monobutyl ether acetate 1%,
Solvent naphtha complements to 100%, obtains the exhibition film oil-suspending agent containing Rynaxypyr and beauveria bassiana.
Embodiment 11
By weight percentage, the beauveria bassiana 4% prepared by embodiment 1, diflubenzuron 36%, nonyl phenol oxygen vinyl Ether
8%, methyl oleate 8%, Lauryl Alcohol ester 2%, octadecanol 5%, propylene glycol phenylate 3%, monobutyl ether acetate 2%, dimethylbenzene is mended
Foot, to 100%, obtains the exhibition film oil-suspending agent containing diflubenzuron and beauveria bassiana.
Embodiment 12
By weight percentage, the beauveria bassiana 8% prepared by embodiment 1, indoxacarb 22%, nonyl phenol oxygen vinyl Ether
6%, methyl oleate 8%, Lauryl Alcohol ester 4%, octadecanol 2%, propylene glycol phenylate 4%, monobutyl ether acetate 1%, white oil is supplied
To 100%, obtain the exhibition film oil-suspending agent containing indoxacarb and beauveria bassiana.
Embodiment 13
By weight percentage, the beauveria bassiana 16% prepared by embodiment 1, Affirm (Merck Co.) 2%, thiacloprid
16%, nonyl phenol oxygen vinyl Ether 12%, Lauryl Alcohol ester 4%, octadecanol 2%, propylene glycol phenylate 2%, monobutyl ether acetate 1%,
Ethanol complements to 100%, obtains the exhibition film oil-suspending agent containing Affirm (Merck Co.), thiacloprid and beauveria bassiana.
Embodiment 14
By weight percentage, the beauveria bassiana 15% prepared by embodiment 1, d ichlorbenzuron 10%, indoxacarb 4%, nonyl
Base phenol oxygen vinyl Ether 15%, Lauryl Alcohol ester 4%, octadecanol 5%, propylene glycol phenylate 2%, monobutyl ether acetate 2%, methyl naphthalene is mended
Foot, to 100%, obtains the exhibition film oil-suspending agent containing Affirm (Merck Co.), thiacloprid and beauveria bassiana.
To embodiment 2～14 obtain the exhibition film oil-suspending agent containing beauveria bassiana carry out surface capability and to prevent and treat white
The test of backward flight louse effect, with 6% Buprofezin film-spreading oil solution for comparison, the results are shown in Table 1：
Table 1 contains exhibition film oil-suspending agent surface capability and the prevention effect test result of beauveria bassiana
As can be drawn from Table 1, using the present invention provide beauveria bassiana open up film oil-suspending agent sprawl the time be 1.8～
2.1s, spreading rate is 68～74cm/s, and spreading area is 5.8～6.5m2, insecticidal effect is more than 84%.With matched group phase
The spreading property of the exhibition film oil-suspending agent of beauveria bassiana is good, and the insecticidal effect reaching is stable and is higher than 6% Buprofezin exhibition for ratio
Film oil preparation.
As seen from the above embodiment, of the present invention exhibition film oil-suspending agent compared with traditional film-spreading oil solution, the present invention
The spreading property of the exhibition film oil-suspending agent providing is good, the effect stability of the preventing and treating insect pest reaching.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of exhibition film oil-suspending agent, including the component of following weight/mass percentage composition：
Effective ingredient 0.1～60%, described effective ingredient includes beauveria bassiana；
Surfactant 1.0～20%；
Film former 2.0～20%；
The oiliness disperse medium of surplus.
2. exhibition film oil-suspending agent according to claim 1 is it is characterised in that spore content of living in described beauveria bassiana is
100～30,000,000,000/gram.
3. exhibition film oil-suspending agent according to claim 1 is it is characterised in that described effective ingredient also includes organic insecticidal
Agent, described organic insecticide is Affirm (Merck Co.), MTI-446, Diacloden, avilamycin, thiacloprid, pleocidin, fluorine
One of pyridine insect amide, d ichlorbenzuron, Rynaxypyr, diflubenzuron, effective cypermethrin, decises and indoxacarb or several
Kind.
4. exhibition film oil-suspending agent according to claim 3 is it is characterised in that described beauveria bassiana and organic insecticide
Weight ratio is (1～45)：(1～55).
5. according to claim 1 exhibition film oil-suspending agent it is characterised in that described film former include Lauryl Alcohol ester, 18
One or more of alcohol, propylene glycol phenylate and monobutyl ether acetate.
6. open up film oil-suspending agent according to claim 1 or 5 it is characterised in that described film former is in exhibition film oil-suspending agent
Weight/mass percentage composition be 5～15%.
7. open up film oil-suspending agent according to claim 1 it is characterised in that described surfactant includes nonyl phenol oxygen ethylene
One of ether and methyl oleate or two kinds.
8. the exhibition film oil-suspending agent according to claim 1 or 7 is it is characterised in that include 5～18% surfactant.
9. exhibition film oil-suspending agent according to claim 1 is it is characterised in that described oiliness disperse medium is white oil, diformazan
One or more of benzene, solvent naphtha, methyl naphthalene and ethanol.
10. exhibition film oil-suspending agent the answering in terms of preventing and treating crops insect pest according to claim 1～9 any one
With.
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| CN201610794422.XA CN106376601A (en) | 2016-08-31 | 2016-08-31 | Spreading oil suspension, and application thereof in control of insect diseases of crops |
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| CN201610794422.XA CN106376601A (en) | 2016-08-31 | 2016-08-31 | Spreading oil suspension, and application thereof in control of insect diseases of crops |
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| CN107926986A (en) * | 2017-11-30 | 2018-04-20 | 江西省科学院微生物研究所 | A kind of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus and muscardine insecticidal suspending agent and its preparation method and application |
| CN108041030A (en) * | 2017-12-05 | 2018-05-18 | 江西省科学院微生物研究所 | A kind of suspending agent and its application containing pyrethroid |
| CN109287664A (en) * | 2018-11-19 | 2019-02-01 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Pesticidal combination and preparation method thereof containing beauveria bassiana and d ichlorbenzuron |
| CN109511682A (en) * | 2018-11-19 | 2019-03-26 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Pesticidal combination and preparation method thereof containing beauveria bassiana |
| WO2019126922A1 (en) * | 2017-12-25 | 2019-07-04 | Dow Global Technologies Llc | Micro-encapsulation of an insecticide |
| CN112400902A (en) * | 2020-10-22 | 2021-02-26 | 湖南顺新龙辰生物工程有限公司 | Pesticide composition |
| CN117598324A (en) * | 2023-11-21 | 2024-02-27 | 西南大学 | Beauveria bassiana-based pesticide for controlling Bactrocera dorsalis and testing method thereof |
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