CN106370874B - A kind of ultramicron nucleic acid-protein analyzer goes up sample testing apparatus automatically - Google Patents
A kind of ultramicron nucleic acid-protein analyzer goes up sample testing apparatus automatically Download PDFInfo
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- CN106370874B CN106370874B CN201610750313.8A CN201610750313A CN106370874B CN 106370874 B CN106370874 B CN 106370874B CN 201610750313 A CN201610750313 A CN 201610750313A CN 106370874 B CN106370874 B CN 106370874B
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- detection probe
- sample
- decelerator
- mandrel
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Robotics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to a kind of ultramicron nucleic acid-protein analyzer to go up sample testing apparatus automatically so that the efficiency and accuracy rate of the detection and analysis of nucleic acid-protein greatly improve.Scheme is, including probe operating mechanism, wiper mechanism, point sample mechanism, sample feeding mechanism, probe operating mechanism is that swinging up and down for detection probe is realized under the control of stepper motor, wiper mechanism drives the first mandrel to rotate under step motor control so that the cleaning paper on the second mandrel is wrapped in the cleaning realized on the first mandrel to upper detection probe and lower detection probe.Point sample mechanism makes sample in sample rifle inject in lower detection probe, and sample feeding mechanism drives moving horizontally for wiper mechanism and point template mechanism.The invention enables the amount control to check analysis sample is more accurate, using automatic control device, the upper pattern detection of automation is realized so that the efficiency and accuracy rate of nucleic acid-protein detection and analysis significantly improve, so that examines is simple to operate, the unnecessary work of related scientific research personnel is reduced.
Description
Technical field
The present invention relates to medical laboratory instrument, more particularly, to a kind of upper sample inspection automatically of ultramicron nucleic acid-protein analyzer
Survey device.
Background technology
On existing ultramicron nucleic acid-protein analyzer, in pattern detection operating process, first having to detection probe
Cleared up, then some circulate operations such as sample detection analysis, cleaning, loading, analysis, cleaning again.Inspector is at this
In operating process, computer is also operated, analysis result is arranged, whole process is cumbersome and easy error.
The content of the invention
For the above situation, to overcome the defect of prior art, the purpose of the present invention is just to provide a kind of ultramicron nucleic acid
Protein analyzer goes up sample testing apparatus automatically, on the basis of existing ultramicron nucleic acid-protein analyzer, increases present invention dress
Put so that the efficiency and accuracy rate of the detection and analysis of nucleic acid-protein greatly improve so that related scientific research personnel are in scientific research
It is more handy.
Its solution is, including probe operating mechanism, wiper mechanism, point sample mechanism, sample feeding mechanism;
The probe operating mechanism includes the first bearing for being fixedly mounted on nucleic acid-protein analyzer upper surface, the first bearing
On be fixed with the first decelerator, the first reducer input shaft is coaxially fixed with the first stepper motor being fixed on the first bearing,
The vertical output shaft of first decelerator is fixed with the driving swing rod for being transversely to the machine direction output shaft axis, one end of the driving swing rod
It is connected on the vertical output shaft of the first decelerator, drives the free end of swing rod to be connected with rod ends, rod ends band is oriented
The cavity that end face is opened, the spring to be stretched along side pole joint axis is fixed with cavity, one end of spring is connected in cavity
Bottom, the free end of spring are connected on the end face of driving swing rod free end, drive the free splaying of swing rod to extend into rod end
In the cavity of joint, the head of rod ends is hinged with detection probe depression bar, is hinged in the middle part of detection probe depression bar with rod ends,
On the bearing of lower hinge first of detection probe depression bar and hinged place is located at the left side of the first reducer output shaft, detection probe pressure
Bar free end is fixed with detection probe;
Automatically upper sample testing apparatus also includes being fixed on nucleic acid-protein point a kind of ultramicron nucleic acid-protein analyzer
The guide-rail plate of analyzer upper surface, be fixed with guide-rail plate laterally the first guide rail and on the left of guide-rail plate with the first guide rail parallel
Laterally the second guide rail;
The wiper mechanism includes the second bearing being slidably connected with the first guide rail, and the second deceleration is fixed with the second bearing
Device, the input shaft of the second decelerator are coaxially fixed with the second stepper motor being fixed on the second bearing, and the second decelerator is indulged
Axis and the first guide rail parallel are coaxially fixed with to output shaft and is also turned positioned at the first mandrel of the first guide upper, the second bearing
It is dynamic to be connected with the second mandrel being longitudinally spaced-apart with the first mandrel, the axis of the second mandrel and the first mandrel diameter parallel
And it is highly identical, the second mandrel, which is fixed with volume, the paper roll of clean cleaning paper, has position between the first mandrel and the second mandrel
Lower detection probe that is fixed and being contacted with the upper detection probe under the effect of detection probe depression bar, the first mandrel and the second core
There are two be rotatably connected on the second bearing and axis and the first mandrel axis in longitudinal both sides above lower detection probe between axle
Parallel pressure roller, cleaning paper is adjacent to be enclosed on the first mandrel around the bottom volume of two pressure rollers, is cleaned below paper under being close to
Detection probe and detection probe can be close to above, second bearing is fixedly connected with the drawing parallel with the first guide rail direction
Bar;
The point sample mechanism include on the left of wiper mechanism and lower detection probe and be connected pull bar and with the second guide rail
The support being slidably connected, it is fixedly connected with support towards inclined of bottom right sample rifle, the 3rd decelerator is fixed with support,
The input shaft of three decelerators is coaxially fixed with the 3rd stepper motor being fixed on support, and the output shaft of the 3rd decelerator is coaxially solid
It is connected with the first nut of axis inclined upward to the left, the first nut screw, which is connected with axis and point sample gun axis line is parallel, is located at point
First leading screw of sample rifle lower left, one end of the first leading screw are screwed in the first nut, and its free end is fixed with vertical first
The pressing plate of thick stick axis, pressing plate are also connected in the point sample rifle piston of its lower right by hand, the head direction of described sample rifle
Lower right and the top of lower detection probe can be located at and under sample is injected under pressing plate function in detection probe, the support
Bottom is fixed with diameter parallel in the second nut of the second guide rail;
The sample feeding mechanism includes the 4th decelerator being connected on guide-rail plate, and the input shaft of the 4th decelerator is coaxially connected
There is the 4th stepper motor being fixed on final drive casing, coaxially connected second leading screw of horizontal output shaft of the 4th decelerator,
Two leading screws are connected with the second nut screw.
The invention enables more accurate to the control of the amount of check analysis sample, using automatic control device, realize automatic
Pattern detection in change so that nucleic acid-protein detection and analysis efficiency and accuracy rate significantly improve so that inspection it is simple to operate, subtract
The unnecessary work of few related scientific research personnel, has very high application and popularization value.
Brief description of the drawings
Fig. 1 is front view schematic diagram of the present invention.
Fig. 2 is top view illustration of the present invention.
Fig. 3 is detection probe operating mechanism schematic diagram of the present invention.
Fig. 4 is wiper mechanism front view schematic diagram of the present invention.
Fig. 5 is wiper mechanism left view schematic diagram of the present invention.
Fig. 6 is point sample mechanism schematic diagram of the present invention.
Fig. 7 is deposit structural scheme of mechanism of the present invention.
Embodiment
The embodiment of the present invention is described in further detail below in conjunction with accompanying drawing.
Provided by Fig. 1 to Fig. 7, a kind of ultramicron nucleic acid-protein analyzer goes up sample testing apparatus automatically, it is characterised in that
Including probe operating mechanism 1, wiper mechanism 2, point sample mechanism 3, sample feeding mechanism 4;
First bearing 9 of the probe operating mechanism including being fixedly mounted on the upper surface of nucleic acid-protein analyzer 5, first
The first decelerator 8 is fixed with seat 9, the input shaft of the first decelerator 8 is coaxially fixed with the first stepping being fixed on the first bearing
Motor 7, the vertical output shaft of the first decelerator 8 are fixed with the driving swing rod 10 for being transversely to the machine direction output shaft axis, the driving
One end of swing rod 10 is connected on the vertical output shaft of the first decelerator 8, and the free end of driving swing rod 10 is connected with rod ends
12, the cavity that rod ends 12 are opened with oriented end face, the spring 11 to be stretched along the axis of side pole joint 12 is fixed with cavity,
One end of spring 11 is connected in the bottom of cavity, and the free end of spring 11 is connected on the end face of driving swing rod 10 free end, driven
The free splaying of driven fork 10 is extend into the cavity of rod ends 12, and the head of rod ends 12 is hinged with detection probe pressure
Bar 6, the middle part of detection probe depression bar 6 is be hinged with rod ends 12, on the first bearing of lower hinge 9 of detection probe depression bar 6 and cuts with scissors
Place is met positioned at the left side of the first reducer output shaft, the free end of detection probe depression bar 6 is fixed with detection probe 13;
Automatically upper sample testing apparatus also includes being fixed on nucleic acid-protein point a kind of ultramicron nucleic acid-protein analyzer
The guide-rail plate 35 of analyzer upper surface, be fixed with guide-rail plate 35 laterally the first guide rail 20 and on the left of guide-rail plate with the first guide rail
20 parallel laterally the second guide rails 30;
The wiper mechanism includes the second bearing 17 for being slidably connected with the first guide rail 20, and the is fixed with the second bearing 17
Two decelerators 16, the input shaft of the second decelerator 16 are coaxially fixed with the second stepper motor 15 being fixed on the second bearing, the
The vertical output shaft of two decelerators 16 is coaxially fixed with axis and the first guide rail parallel and positioned at the first core of the first guide upper
Axle 18, the second bearing 17 are also rotatably connected to the second mandrel 23 with the 18 parallel and longitudinally spaced setting of the first mandrel, the second core
Axle 23, which is fixed with volume, the paper roll 24 of clean cleaning paper, have that position fixes between the first mandrel 18 and the second mandrel 23 and energy
The lower detection probe 14 contacted with the upper detection probe 13 under the effect of detection probe depression bar, the first mandrel 18 and the second mandrel 23
Between the longitudinal both sides in the lower top of detection probe 14 have two be rotatably connected on the second bearing 17 and axis and the first mandrel 18
The pressure roller 20 of diameter parallel, cleaning paper is adjacent to be enclosed on the first mandrel 18 around the bottom volume of two pressure rollers 20, is cleaned below paper
Lower detection probe 14 can be close to and detection probe 13 can be close to above, second bearing 17 is fixedly connected with and first
The parallel pull bar 21 in the direction of guide rail 20;
The point sample mechanism is included positioned at wiper mechanism 2 and the left side of lower detection probe 14 and the pull bar 21 and with second of being connected
The support 22 that guide rail 30 is slidably connected, it is fixedly connected with support towards inclined of bottom right sample rifle, is fixed with support 22
Three decelerators 28, the input shaft of the 3rd decelerator 28 are coaxially fixed with the 3rd stepper motor 29 being fixed on support 22, and the 3rd
The output shaft of decelerator 28 is coaxially fixed with the first nut of axis inclined upward to the left, the first nut screw be connected with axis with
For the diameter parallel of point sample rifle 25 positioned at the first leading screw 27 of point sample rifle lower left, one end of the first leading screw 27 screws in the first nut
In, its free end is fixed with the pressing plate 26 of vertical first leading screw axis, and pressing plate 26 is also connected in the point sample rifle 25 of its lower right
For piston by hand, the head of described sample rifle 25 is towards lower right and can be located at the top of lower detection probe and in pressing plate 26
Sample is injected in lower detection probe 14 under effect, the bottom of support 22 is fixed with diameter parallel in the second of the second guide rail 30
Nut 34;
The sample feeding mechanism includes the 4th decelerator 32 being connected on guide-rail plate 35, and the input shaft of the 4th decelerator 32 is same
Axle is fixed with the 4th stepper motor 33 being fixed on final drive casing, the horizontal output shaft coaxially connected of the 4th decelerator 32
Two leading screws 31, the second leading screw 31 are connected with the second nut screw 34.
The present invention can be used in the entitled Thermo SCIENTIFIC of INSTRUMENT MODEL:NANODROP 1000
On Spectrophotometer ultramicron nucleic acid-protein analyzer
The present invention in use, four stepper motors in the control of SCM program, realize corresponding action, detection when
Between, interval is controllable.Sample sample is placed in a sample rifle 25, the 4th stepper motor 33 drives the second screw mandrel
31 rotations cause nut 34 and support 22 to be moved along 30 downward detection probe direction of the second guide rail, and then by being connected to support
Pull bar 21 on 22 drives wiper mechanism 2 to be moved to same direction so that entirely lower detection probe 14 is completely covered in cleaning paper.
Then the 4th stepper motor 33 is stopped, and the first stepper motor 7 starts so that detection probe depression bar 6 turns to detection and visited
The first stepper motor 7 is stopped after first 13 contact cleaning paper, and the second stepper motor 15 works, and drives the first mandrel 18 to rotate,
So that clean paper roll on the first mandrel 18, then will be by upper inspection by the cleaning paper of upper detection probe 13 and lower detection probe 14
Probing first 13 and lower detection probe 14 clean up.After cleaning terminates, the second stepper motor 15 is stopped, the first stepper motor
Work starts 7 and causes detection probe depression bar 6 to be raised up to certain altitude, and then the first stepper motor 7 is stopped, the 4th stepping electricity
Machine 33 works so that sample presentation mechanism 3 and wiper mechanism 2 continue to move right along guide rail direction, when a head for sample rifle 25
When above lower detection probe 14, and now wiper mechanism is located at the right side of lower detection probe 14, and the 4th stepper motor 33 stops
Only work, the electric motor starting of the 3rd stepping 29, the 3rd stepper motor 29 drives the rotation of the first nut so that the first leading screw 27 and pressing plate
26 move along lower right, the point sample in lower detection probe 14 of point sample rifle 25.After point sample terminates, the 3rd stepper motor 29 stops
Only work, the 4th stepper motor 33 works so that sample presentation mechanism 3 and wiper mechanism 2 are moved to the left certain distance, now clean
The cleaning paper of mechanism is still located on the lower right side of detection probe 14.4th stepper motor 33 is stopped, the work of the first stepper motor 7
Make so that detection probe depression bar 6 is depressed, and then contacts detection probe 13 and lower detection probe 14, to sample pattern detection.
After detection terminates, the first stepper motor 7 works so that detection probe depression bar 6 lifts, and then the first stepper motor 7 is stopped
Then the 4th motor work 33 causes sample presentation mechanism 3 and wiper mechanism 2 to be moved to the left the lower detection probe 14 of cleaning paper covering,
4th stepper motor 33 is stopped, and the first stepper motor work 7 causes the pressure contact cleaning paper of detection probe depression bar 6, then
First stepper motor 7 is stopped, and the second stepper motor 15 works, and upper detection probe 13 and lower detection probe 14 are carried out clearly
Wash, the second stepper motor 15 is stopped after cleaning terminates, and depression bar 6 lifts after detection is visited, sample presentation mechanism 3 and wiper mechanism 2
Original position is moved to the left, a working cycles is completed, repeats said process when examining for the second time, fully achieve in automation
Pattern detection.Because it is to realize elastic connection by spring to drive swing rod and rod ends, can be protected in detection process
Upper detection probe and lower detection probe are not subject to crushing, and point sample mechanism realizes that Automated condtrol ensure that the dosage essence of sample sample
Really so that test result is more reliable
Embodiment described above is not defined to the scope of the present invention, before design concept of the present invention is not departed from
Put, the various modifications and improvement that technical staff described in this area makes to technical scheme, the present invention all should be included
Claims determine protection domain in.
Claims (1)
1. a kind of ultramicron nucleic acid-protein analyzer goes up sample testing apparatus automatically, it is characterised in that including operating mechanism of popping one's head in
(1), wiper mechanism(2), point sample mechanism(3), sample feeding mechanism(4);
The probe operating mechanism(1)Including being fixedly mounted on nucleic acid-protein analyzer(5)First bearing of upper surface(9), the
One bearing(9)On be fixed with the first decelerator(8), the first decelerator(8)Input shaft, which is coaxially fixed with, is fixed on the first bearing(9)
On the first stepper motor(7), the first decelerator(8)Vertical output shaft be fixed with the driving for being transversely to the machine direction output shaft axis
Swing rod(10), the driving swing rod(10)One end be connected in the first decelerator(8)Vertical output shaft on, drive swing rod(10)
Free end be connected with rod ends(12), rod ends(12)The cavity opened with oriented end face, edge is fixed with cavity
Side pole joint(12)The flexible spring of axis(11), spring(11)One end be connected in the bottom of cavity, spring(11)Freedom
End is connected in driving swing rod(10)On the end face of free end, swing rod is driven(10)Free splaying extend into rod ends(12)
Cavity in, rod ends(12)Head be hinged with detection probe depression bar(6), detection probe depression bar(6)Middle part connects with rod end
Head(12)It is be hinged, detection probe depression bar(6)The bearing of lower hinge first(9)Upper and hinged place is located at the first decelerator(8)It is defeated
The left side of shaft, detection probe depression bar(6)Free end is fixed with detection probe(13);
Automatically upper sample testing apparatus also includes being fixed on nucleic acid-protein analyzer a kind of ultramicron nucleic acid-protein analyzer
The guide-rail plate of upper surface(35), guide-rail plate(35)On be fixed with laterally the first guide rail(20)With positioned at guide-rail plate(35)Left side and the
One guide rail(20)Parallel laterally the second guide rail(30);
The wiper mechanism(2)Including with the first guide rail(20)The second bearing being slidably connected(17), the second bearing(17)It is upper solid
It is connected with the second decelerator(16), the second decelerator(16)Input shaft be coaxially fixed with and be fixed on the second bearing(17)On second
Stepper motor(15), the second decelerator(16)Vertical output shaft be coaxially fixed with axis and the first guide rail parallel and positioned at first
Guide rail(20)First mandrel on top(18), the second bearing(17)Also it is rotatably connected to and the first mandrel(18)Between parallel and longitudinal direction
Every the second mandrel of setting(23), the second mandrel(23)Being fixed with volume has the paper roll of clean cleaning paper(24), the first mandrel
(18)With the second mandrel(23)Between have position fix and can with detection probe depression bar(6)Upper detection probe under effect
(13)The lower detection probe of contact(14), the first mandrel(18)With the second mandrel(23)Between lower detection probe(14)Top longitudinal direction
Both sides have two to be rotatably connected on the second bearing(17)On and axis and the first mandrel(18)The pressure roller of diameter parallel(20), clearly
Paper is washed to be adjacent to around two pressure rollers(20)Bottom volume be enclosed on the first mandrel(18)On, lower detection can be close to by, which cleaning below paper, visits
Head(14)And detection probe can be close to above(13), second bearing(17)It is fixedly connected with and the first guide rail(20)Side
To parallel pull bar(21);
The point sample mechanism(3)Including positioned at wiper mechanism(2)With lower detection probe(14)Left side and the pull bar that is connected(21)And
With the second guide rail(30)The support being slidably connected(22), support(22)On be fixedly connected with towards inclined of bottom right sample rifle
(25), support(22)On be fixed with the 3rd decelerator(28), the 3rd decelerator(28)Input shaft be coaxially fixed with and be fixed on branch
Frame(22)On the 3rd stepper motor(29), the 3rd decelerator(28)Output shaft be coaxially fixed with axis inclined upward to the left
First nut, the first nut screw are connected with axis with putting sample rifle(25)Diameter parallel is positioned at a sample rifle(25)Lower left
First leading screw(27), the first leading screw(27)One end screw in the first nut in, its free end is fixed with vertical first leading screw axis
Pressing plate(26), pressing plate(26)Also it is connected in the point sample rifle of its lower right(25)Piston is pressed on hand, described sample rifle(25)
Head towards lower right and lower detection probe can be located at(14)Top and in pressing plate(26)Under sample is injected under effect
Detection probe(14)In, the support(22)Bottom is fixed with diameter parallel in the second guide rail(30)The second nut(34);
The sample feeding mechanism(4)Including being connected in guide-rail plate(35)On the 4th decelerator(32), the 4th decelerator(32)It is defeated
Enter axle and be coaxially fixed with the 4th stepper motor being fixed on final drive casing(33), the 4th decelerator(32)Horizontal output shaft
Coaxially connected second leading screw(31), the second leading screw(31)With the second nut screw(34)Connection.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610750313.8A CN106370874B (en) | 2016-08-27 | 2016-08-27 | A kind of ultramicron nucleic acid-protein analyzer goes up sample testing apparatus automatically |
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CN201610750313.8A CN106370874B (en) | 2016-08-27 | 2016-08-27 | A kind of ultramicron nucleic acid-protein analyzer goes up sample testing apparatus automatically |
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Publication Number | Publication Date |
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CN106370874A CN106370874A (en) | 2017-02-01 |
CN106370874B true CN106370874B (en) | 2017-11-17 |
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CN201610750313.8A Expired - Fee Related CN106370874B (en) | 2016-08-27 | 2016-08-27 | A kind of ultramicron nucleic acid-protein analyzer goes up sample testing apparatus automatically |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1878496A1 (en) * | 2006-07-14 | 2008-01-16 | Roche Diagnostics GmbH | Apparatus for performing nucleic acid analysis |
CN103282782A (en) * | 2010-10-27 | 2013-09-04 | 株式会社百奥尼 | Automatic real-ime pcr system for the various analysis of biological smaple |
CN103305412A (en) * | 2013-05-31 | 2013-09-18 | 广州安必平自动化检测设备有限公司 | Full-automatic nucleic acid extraction machine |
CN203929772U (en) * | 2014-06-27 | 2014-11-05 | 珠海科域生物工程有限公司 | A kind of sampling point sample cleaning device |
-
2016
- 2016-08-27 CN CN201610750313.8A patent/CN106370874B/en not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1878496A1 (en) * | 2006-07-14 | 2008-01-16 | Roche Diagnostics GmbH | Apparatus for performing nucleic acid analysis |
CN103282782A (en) * | 2010-10-27 | 2013-09-04 | 株式会社百奥尼 | Automatic real-ime pcr system for the various analysis of biological smaple |
CN103305412A (en) * | 2013-05-31 | 2013-09-18 | 广州安必平自动化检测设备有限公司 | Full-automatic nucleic acid extraction machine |
CN203929772U (en) * | 2014-06-27 | 2014-11-05 | 珠海科域生物工程有限公司 | A kind of sampling point sample cleaning device |
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