CN106367326B - A kind of device of immobilized cell continuous production extraction pentanediamine - Google Patents
A kind of device of immobilized cell continuous production extraction pentanediamine Download PDFInfo
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- CN106367326B CN106367326B CN201610609856.8A CN201610609856A CN106367326B CN 106367326 B CN106367326 B CN 106367326B CN 201610609856 A CN201610609856 A CN 201610609856A CN 106367326 B CN106367326 B CN 106367326B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C209/00—Preparation of compounds containing amino groups bound to a carbon skeleton
- C07C209/82—Purification; Separation; Stabilisation; Use of additives
- C07C209/86—Separation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
Abstract
The invention discloses the devices that a kind of immobilized cell continuous production extracts pentanediamine, part, column absorb-elute part and Centrifugical extraction part are adjusted including feedstock transportation part, pentanediamine transform portion, PH, head tank, pentanediamine conversion integrated tank and phytic acid calcium column are connected by circulation by pipeline, PH is adjusted on the pipeline that flow container is connected to by pipeline between head tank and pentanediamine conversion integrated tank, eluent holding vessel connects phytic acid calcium column by pipeline, and centrifugal extractor connects on phytic acid calcium column.Compared with prior art; a kind of device of immobilized cell continuous production extraction pentanediamine of the present invention; can preferably solve the problems, such as the extraction of pentanediamine continuous production; large-scale production suitable for pentanediamine operates, which has the characteristics that easy to operate, utilization rate of equipment and installations is high, product with stable quality, raw material make full use of, are easy to spread.
Description
Technical field
The present invention relates to a kind of production extraction equipment of compound, especially a kind of immobilized cell continuous production extracts penta
The device of diamines belongs to industrial processes production technical field.
Background technology
Pentanediamine (cadaverine, alias cadaverine) is the fuming liquids of colorless viscous, there is special odor.Fusing point：9
DEG C, it is dissolved in water, ethyl alcohol etc..Pentanediamine monomer is a kind of intermediate having important and extensive industrial use, is widely used in each
Kind polyamide product（Nylon）, chelating agent and additive etc..With the raising of environmental consciousness and the rise of biological economy, as flat
The pentanediamine of platform compound is increasingly paid attention to.The pentanediamine of biological method mass production can be substituted and traditional be given birth to by chemical industry
Biogenic amine-hexamethylene diamine of production, high-quality high molecular material-novel nylon can be synthesized by carrying out polymerisation with binary organic acid, be
A kind of environment-friendly type, sustainable development, heat safe biological plastics, have a wide range of applications.Traditional chemistry closes
, pollution environment harsh at law article part, is highly dependent on petrochemical industry.Resting cell production pentanediamine has economy, ring
The advantages of guarantor, sustainable development.The pentanediamine of traditional resting cell production pentanediamine high concentration is larger to cytotoxic, carefully
Born of the same parents cannot reuse, and separation is complex.
Invention content
The technical problem to be solved in the invention be in view of the above shortcomings of the prior art, and provide it is a kind of it is easy to operate,
The high pentanediamine of utilization rate of equipment and installations produces extraction equipment.
In order to solve the above technical problems, the technical solution adopted by the present invention is：
A kind of device of immobilized cell continuous production extraction pentanediamine, including feedstock transportation part, pentanediamine conversion portion
Divide, PH adjusts part, column absorb-elute part and Centrifugical extraction part；Feedstock transportation part includes head tank；Pentanediamine turns
It includes that pentanediamine converts integrated tank to change part, has immobilized cell bacterial spawn in the pentanediamine conversion integrated tank；PH adjustment portions
Divide includes that PH adjusts flow container；Column absorb-elute part includes eluent holding vessel and phytic acid calcium column；Centrifugical extraction part include from
Heart extracter；The head tank, pentanediamine conversion integrated tank and phytic acid calcium column are connected by circulation by pipeline, and the PH adjusts flow container
It is connected on the pipeline between head tank and pentanediamine conversion integrated tank by pipeline, the eluent holding vessel is connected by pipeline
Phytic acid calcium column is connect, the centrifugal extractor connects on phytic acid calcium column.
As further preferred embodiment, having heaters is set between the head tank and pentanediamine conversion integrated tank.
As further preferred embodiment, the feedstock transportation part further include the first delivery pump, the first solenoid valve and
Second solenoid valve；The head tank, the first delivery pump, the first solenoid valve and second solenoid valve are connected by circulation by pipeline, described
Heater is connected to by pipeline on the pipeline between the first solenoid valve and second solenoid valve.
As further preferred embodiment, it further includes the second delivery pump, third solenoid valve and that the PH, which adjusts part,
Four solenoid valves；The PH adjusts flow container, the second delivery pump, third solenoid valve and the 4th solenoid valve and is connected by circulation by pipeline, institute
It states on the pipeline that heater is connected to by pipeline between third solenoid valve and the 4th solenoid valve.
As further preferred embodiment, column absorb-elute part further includes third delivery pump, the 5th solenoid valve
With the 6th solenoid valve；The eluent holding vessel, third delivery pump, the 5th solenoid valve and the 6th solenoid valve are recycled by pipeline to be connected
It connects, the phytic acid calcium column is connected to by pipeline on the pipeline between the 5th solenoid valve and the 6th solenoid valve.
As further preferred embodiment, the phytic acid calcium column is divided into the first calcium column and the second calcium column, perforation the
The first isocon and the 4th isocon is arranged in one calcium column, and the second isocon and third isocon is arranged in the second calcium column of perforation；It is described
First calcium column and the first isocon on second calcium column the same end and the second isocon connect eluent holding vessel, the other end jointly
On the first isocon and the second isocon connect Centrifugical extraction part jointly；On first calcium column and second calcium column the same end
Four isocons and third isocon connect pentanediamine conversion integrated tank, the 4th isocon on the other end and third isocon jointly
Common connection head tank；It is respectively set on first isocon, the 4th isocon, the second isocon and third isocon useful
In the valve of control switch.
As further preferred embodiment, be additionally provided on the pentanediamine conversion integrated tank PH detection feedback systems,
Temperature detection reponse system, chuck and lysine concentration detection device.
As further preferred embodiment, the Centrifugical extraction part further includes the extractant being connected by circulation by pipeline
Holding vessel, the 4th delivery pump, the 7th solenoid valve and the 8th solenoid valve, the centrifugal extractor are connected to the 7th electromagnetism by pipeline
On pipeline between valve and the 8th solenoid valve.
As further preferred embodiment, the Centrifugical extraction part further includes that raffinate collecting tank and extract liquor are collected
Tank.
As further preferred embodiment, the Centrifugical extraction part is at least provided with three centrifugal extractors.
Advantageous effect
Compared with prior art, the device of a kind of immobilized cell continuous production extraction pentanediamine of the invention, can be compared with
Solve the problems, such as well pentanediamine continuous production extraction, bacterium cell is converted using material liquid, obtain concentration it is relatively low and
It is mixed in the pentanediamine of solution, then the higher pentanediamine of content is obtained by elution and Centrifugical extraction, the present apparatus is suitable for penta 2
The large-scale production of amine operates, the device have easy to operate, utilization rate of equipment and installations is high, product with stable quality, raw material make full use of,
The features such as easy to spread, specifically has the following advantages：
Raw material availability is high, production efficiency is high, more environmentally-friendly；
It is adjusted using on-line checking reponse system, it is easy to operate to adjust rapidly；
Utilization rate of equipment and installations is high, economic security；
Production process is closed, safety and sanitation；
Product with stable quality.
Description of the drawings
Fig. 1 is the structural schematic diagram of the present invention；
Fig. 2 is the flow chart of the present invention；
Fig. 3 is distribution map of the isocon on phytic acid calcium column；
Fig. 4 is the structural schematic diagram of pentanediamine conversion integrated tank；
Wherein, 11- head tanks, the first solenoid valves of 12-, 13- second solenoid valves, the first delivery pumps of 14-, 21- pentanediamines turn
Change integrated tank, 22-PH detection feedback systems, 23- temperature detection reponse systems, 24- chucks, 25- immobilized cell bacterial spawns, 26-
Lysine concentration detection device, 31-PH adjusting flow containers, 32- third solenoid valves, the 4th solenoid valves of 33-, the second delivery pumps of 34-,
41- eluent holding vessels, the 5th solenoid valves of 42-, the 6th solenoid valves of 43-, 44- third delivery pumps, 51- centrifugal extractors, 52- extractions
Take solvent holding vessel, the 7th solenoid valves of 53-, the 8th solenoid valves of 54-, the 4th delivery pumps of 55-, 56- raffinate collecting tanks, 57- extractions
Take liquid collecting tank, 61- heaters, 71- the first calcium columns, 72- the second calcium columns, the first isocons of 73-, the 4th isocons of 74-, 75-
Second isocon, 76- third isocons.
Specific implementation mode
The optimal technical scheme that the invention will now be described in detail with reference to the accompanying drawings.
As shown, a kind of device of immobilized cell continuous production extraction pentanediamine of the present invention, including feedstock transportation
Partly, pentanediamine transform portion, PH adjust part, column absorb-elute part and Centrifugical extraction part；
Production pentanediamine bacterial strain refers to patent《A kind of genetic engineering bacterium and its purposes in producing 1,5- pentanediamines》Application
Number CN2014107999948.8, method for immobilizing cell refer to《Patent it is a kind of tolerance toxic products method for immobilizing cell and
Its immobilized cell produces 1,5- pentanediamine techniques》Application number 201610368936.9.
Feedstock transportation part includes head tank 11, the material liquid in head tank 11 be lysine salt solution, PH be 5.0~
8.5, preferably 6.6~7.0；
Pentanediamine transform portion includes pentanediamine conversion integrated tank 21, is additionally provided on the pentanediamine conversion integrated tank 21
PH detection feedback systems 22, temperature detection reponse system 23, chuck 24 and lysine concentration detection device 26, pentanediamine conversion one
Immobilized cell bacterial spawn 25 in body tank 21, immobilized cell material therefor, which is polymer carrier, can enumerate such as fine jade
Fat, carragheen, alginate, cellulose, gelatin, collagen polyacrylamide, polyvinyl alcohol, polyurethane foam, light-cured resin
Deng；Inorganic carrier material can be enumerated such as kaolin, porous silicon, activated carbon, cellular glass, aluminium oxide；Composite carrier
Such as sodium alginate/SiO can be enumerated2Composite hydrogel, calcium alginate, polyethylene glycol, polyvinyl lactam compounding carrier, κ-card
Draw glue and konjac polysaccharide compound gel, gelatin, agar, sodium alginate polyvinyl alcohol composite carrier etc..Above-mentioned material can be used alone
Or it is used in combination two or more；The lysine concentration detection device 26 of pentanediamine conversion integrated tank 21 detects lysine content（g/l）
It is 0~60, preferably 00~30；
PH adjustment portions point include that PH adjusts flow container 31, and the feed liquid that PH adjusts batch can 31 is acid solution, molten as acidity
Liquid can enumerate such as hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, sulfurous acid, hydrofluoric acid, carbonic acid, hydrosulphuric acid, boric acid, formic acid, acetic acid, third
Acid, butyric acid, octanoic acid, adipic acid, ethanedioic acid, malonic acid, succinic acid, maleic acid, tartaric acid, benzoic acid, phenylacetic acid, O-phthalic
Acid, terephthalic acid (TPA), valeric acid, caproic acid, capric acid, stearic acid, palmitic acid, acrylic acid etc..Above-mentioned acid solution can be used alone or
It is used in combination two or more；
Feedstock transportation part and PH adjust part and are separately connected heater 61 by pipeline, and heater 61 is directly connected to two
Amine transform portion；
Column absorb-elute part includes eluent holding vessel 41 and phytic acid calcium column, in order to ensure good elution effect elution
Liquid holding vessel 41, the PH of eluent is 4.5~13.4 in eluent holding vessel 41；
Centrifugical extraction part includes centrifugal extractor 51, and the Centrifugical extraction part is at least provided with three centrifugal extractors
51；
The head tank 11, pentanediamine conversion integrated tank 21 and phytic acid calcium column are connected by circulation by pipeline, and the PH is adjusted
Flow container 31 is connected to by pipeline on the pipeline between head tank 11 and pentanediamine conversion integrated tank 21, the eluent holding vessel
41 connect phytic acid calcium column by pipeline, and the centrifugal extractor 51 connects on phytic acid calcium column；The head tank 11 turns with pentanediamine
Having heaters 61 is set between change integrated tank 21.
Further, the feedstock transportation part further includes the first solenoid valve 12, second solenoid valve 13 and the first delivery pump
14；It the head tank 11, the first delivery pump 14, the first solenoid valve 12 and second solenoid valve 13 and is connected by circulation by pipeline, institute
It states on the pipeline that heater 61 is connected to by pipeline between the first solenoid valve 12 and second solenoid valve 13, is formed into a loop, this is
Make head tank 11 can be by the first delivery pump 14, the first solenoid valve 12 and second solenoid valve 13 again after discharging excessive raw material
Head tank 11 is returned, avoids wasting.
Further, it further includes third solenoid valve 32, the 4th solenoid valve 33 and the second delivery pump 34 that the PH, which adjusts part,；
The PH adjusts flow container 31, the second delivery pump 34, third solenoid valve 32 and the 4th solenoid valve 33 and is connected by circulation by pipeline, described
Heater 61 is connected to by pipeline on the pipeline between third solenoid valve 32 and the 4th solenoid valve 33, and forming circuit, this is to make
PH adjusts flow container 31 can pass through the second delivery pump 34, third solenoid valve 32 and the 4th solenoid valve after discharging excessive adjusting liquid
33, which return again to PH, adjusts flow container 31, avoids wasting.
Further, column absorb-elute part further includes the 5th solenoid valve 42, the 6th solenoid valve 43 and third conveying
Pump 44；The eluent holding vessel 41, third delivery pump 44, the 5th solenoid valve 42 and the 6th solenoid valve 43 are recycled by pipeline to be connected
It connecing, the phytic acid calcium column is connected to by pipeline on the pipeline between the 5th solenoid valve 42 and the 6th solenoid valve 43, forming circuit,
This is that eluent holding vessel 41 is allow to pass through third delivery pump 44, the 5th solenoid valve 42 and the 6th in the excessive eluent of release
Solenoid valve 43 returns again to eluent holding vessel 41, avoids wasting.
Further, the phytic acid calcium column is divided into the first calcium column 71 and the second calcium column 72, the first calcium column 71 of perforation setting the
The second isocon 75 and third isocon 76 is arranged in one isocon 73 and the 4th isocon 74, the second calcium column 72 of perforation；Described
The first isocon 73 and the second isocon 75 on 72 the same end of one calcium column 71 and the second calcium column connect eluent holding vessel jointly
41, the first isocon 73 and the second isocon 75 on the other end connection Centrifugical extraction part jointly；First calcium column 71 and second
Connection pentanediamine converts integrated tank 21 jointly for the 4th isocon 74 and third isocon 76 on 72 the same end of calcium column, on the other end
The 4th isocon 74 and third isocon 76 jointly connection head tank 11；First isocon 73, the 4th isocon 74,
The valve of control switch is separately provided on two isocons 75 and third isocon 76.
Further, the Centrifugical extraction part further includes the extractant holding vessel the 52, the 7th being connected by circulation by pipeline
Solenoid valve 53, the 8th solenoid valve 54 and the 4th delivery pump 55, the centrifugal extractor 51 are connected to the 7th solenoid valve by pipeline
53 and the 8th pipeline between solenoid valve 54 on, forming circuit, this is that extractant holding vessel 52 is made to discharge excessive extraction
Solvent can return again to extractant holding vessel 52 by the 4th delivery pump 55, the 7th solenoid valve 53 and the 8th solenoid valve 54, keep away
Exempt to waste；The Centrifugical extraction part further includes raffinate collecting tank 56 and extract liquor collecting tank 57.
In use, material liquid lysine salt solution is discharged from head tank 11, integrated tank 21 is converted into pentanediamine, with penta 2
The contact of immobilized cell bacterial spawn 25 in amine conversion integrated tank 21 forms pentanediamine, passes through on pentanediamine conversion integrated tank 21
PH detection feedback systems 22 detect, when PH is too high or too low, PH adjust flow container 31 then start release adjust liquid to material liquid into
Row is adjusted, and temperature detection reponse system 23 is detected, and when temperature is less than 36 degree, heater 61 starts, to subsequently entering penta
Diamines converts the material liquid heat treatment of integrated tank 21, and chuck 24 can keep equalized temperature, and temperature is allowed to be in suitable model
It encloses, lysine concentration detection device 26 can detect lysine concentration in pentanediamine conversion integrated tank 21, contain pentanediamine raw material
Liquid discharges into phytic acid calcium column from pentanediamine conversion integrated tank 21, and the first calcium column 71 and the second calcium column 72 in turn carry out pentanediamine
Elution procedure and absorption process enter after the first calcium column 71 containing pentanediamine material liquid and carry out pentanediamine absorption, and the second calcium
Column 72 injects eluent by eluent holding vessel 41 and carries out pentanediamine elution, just by eluting after the first calcium column 71 adsorbs pentanediamine
Liquid holding vessel 41 injects eluent and carries out pentanediamine elution, and material liquid then enters the second calcium column 72 and carries out pentanediamine absorption, instead
Multiple cycle operation, the first isocon 73 are turned on and off with the 4th isocon 74 under the control of valve simultaneously, the second isocon
75 are turned on and off with third isocon 76 under the control of valve simultaneously, when the first isocon 73 and the 4th isocon 74 are opened
When, the second isocon 75 is in third isocon 76 closes, when the second isocon 75 is in third isocon 76 to be opened,
Then the first isocon 73 and the 4th isocon 74, which are in, closes, and the solution containing pentanediamine then enters centrifugal extractor after elution
51, material liquid is adsorbed on after the first calcium column 71 and the second calcium column 72 to be come back to the preparation of head tank 11 and recycles again again, pentanediamine
Solution carries out centrifugal treating in centrifugal extractor 51, and extractant holding vessel 52 injects extract liquor, centrifugation to centrifugal extractor 51
The pentanediamine extraction solution of high concentration enters extract liquor collecting tank 57 after processing, and raffinate then enters raffinate collecting tank 56.
Embodiment 1
The method of the immobilized cell of referenced patents and step immobilized cell are uniformly placed on pentanediamine conversion integrated tank
In 21 immobilized cell bacterial spawn 25.To ensure that immobilized cell has larger contact area, immobilized cell bacterial spawn 25 is by more
The uniform netted column box of stainless steel wire of layer assembles.The power that temperature detection reponse system 23 regulates and controls heater 61 ensures to enter
The lysine salt solution temperature that pentanediamine converts integrated tank 21 is 37 DEG C, and PH detection feedback systems 22 adjust 32 He of third solenoid valve
4th solenoid valve 33 ensures every time identical into the ph of the lysine salt solution of pentanediamine conversion integrated tank 21.In feed liquid in proportion
3：1000（mmol：ml）Phosphopyridoxal pyridoxal phosphate is added, by adjusting the first solenoid valve 12 and second solenoid valve 13, feed change tank 11
Flow, lysine concentration detection device 26 detects remaining lysine concentration in pentanediamine conversion integrated tank 21, now by material liquid
By centrifuging diluted processing vapor detection pentanediamine concentration, obtains data by calculation processing and be shown in Table one：
Table one
| Flow | Lysine salt concentration | Pentanediamine concentration | Every gram of cell produces amine amount |
| 200.0ml/h | 250.0g/l | 154.73g/l | 6.19g/g |
| 250.0ml/h | 250.0g/l | 106.23g/l | 4.2 g/g |
| 300.0ml/h | 250.0g/l | 92.13 g/l | 3.67g/g |
Embodiment 2
When solution containing pentanediamine flows through phytic acid calcium column, the pentanediamine in solution can be adsorbed on phytic acid calcium by phytic acid calcium column
On column, the concentration of pentanediamine in solution is reduced, while reducing the ph of solution.But the not sufficiently stable absorption of absorption in this, warp
It crosses experiment to find to be eluted the part pentanediamine that phytic acid calcium column adsorbs with solution, the phytic acid calcium column after elution is highly concentrated
Degree pentanediamine conversion fluid can adsorb pentanediamine again again when flowing through, and achieve the purpose that recycling.First use same concentrations identical
The pentanediamine conversion fluid of volume flows through phytic acid calcium column and adsorbs pentanediamine, then is being protected respectively with the PH solution for being 4.5,7.0,13.4
It demonstrate,proves identical flow velocity and goes down to elute pentanediamine on phytic acid calcium column.It is dense before and after flowing through phytic acid column with vapor detection solution respectively
The concentration of pentanediamine, calculation processing obtain data and are shown in Table two in degree, eluent：
Table two
| The PH of eluent | Phytic acid calcium column pentanediamine amount | The pentanediamine amount of eluent | Eluting rate |
| 4.5 | 93.18mg | 48.23mg | 51.76% |
| 7.0 | 88.23mg | 52.87mg | 59.92% |
| 13.4 | 90.15mg | 57.66mg | 63.96% |
Embodiment 3
Three extraction centrifuges are first opened in order, and the frequency of regulation motor is 45hz, waits extraction centrifuge to stablize normal
Afterwards, first heavy phase is pumped into from heavy phase import with the flow of 100ml/min, when there is liquid outflow in the outlet of heavy phase, at once with
The flow of 100ml/min is pumped into light phase from light phase import, after waiting heavy and lights two-phase outlet stabilization to go out 15~20min of liquid, starts to remember
Heavy phase, the volume of light phase are recorded, and collects heavy and light two-phase outlet liquid again and measures volume, is simultaneously closed off after running a period of time
The pump of heavy and light two-phase entrance exports the motor of the closing centrifugal extractor not when there is liquid outflow when heavy and light two-phase, finally puts
Fall seldom quantity of fluid in centrifugal extractor.Measure feed liquid（Heavy phase into）, extractant（Light phase into）, extract liquor（Light phase goes out）,
Raffinate（Heavy phase goes out）Volume, their pentanediamine contents of vapor detection.
Three extraction centrifuges are first opened in order, and the frequency of regulation motor is 45hz, waits extraction centrifuge to stablize normal
Afterwards, first heavy phase is pumped into from heavy phase import with the flow of 200ml/min, when there is liquid outflow in the outlet of heavy phase, at once with
The flow of 200ml/min is pumped into light phase from light phase import, after waiting heavy and lights two-phase outlet stabilization to go out 15~20min of liquid, starts to remember
Heavy phase, the volume of light phase are recorded, and collects heavy and light two-phase outlet liquid again and measures volume, is simultaneously closed off after running a period of time
The pump of heavy and light two-phase entrance exports the motor of the closing centrifugal extractor not when there is liquid outflow when heavy and light two-phase, finally puts
Fall seldom quantity of fluid in centrifugal extractor.Measure feed liquid（Heavy phase into）, extractant（Light phase into）, extract liquor（Light phase goes out）,
Raffinate（Heavy phase goes out）Volume, vapor detection pentanediamine content.
Twice three are shown in Table after data preparation
Table three
| Flow | Extract liquor pentanediamine content | Raffinate pentanediamine content | Extract raffinate volume ratio | Extraction yield |
| 100ml/min | 141.18 | 21.34 | 10:7 | 90.43% |
| 200ml/min | 166.34 | 69.73 | 7:10 | 62.64% |
The present invention can also be the series connection of multiple pentanediamine conversion integrated tanks, series connection and shared form in parallel.
Certainly, the present invention can also have other various embodiments, without deviating from the spirit and substance of the present invention, ripe
It knows those skilled in the art and makes various corresponding change and deformations, but these corresponding changes and change in accordance with the present invention
Shape should all belong to the protection domain of appended claims of the invention.
Claims (10)
1. a kind of device of immobilized cell continuous production extraction pentanediamine, it is characterised in that：Including feedstock transportation part, penta 2
Amine transform portion, PH adjust part, column absorb-elute part and Centrifugical extraction part；Feedstock transportation part includes head tank
（11）；Pentanediamine transform portion includes pentanediamine conversion integrated tank（21）, the pentanediamine conversion integrated tank（21）It is interior to have admittedly
Surely change cell bacterial spawn（25）；PH adjustment portions point include that PH adjusts flow container（31）；Column absorb-elute part includes eluent holding vessel
（41）With phytic acid calcium column；Centrifugical extraction part includes centrifugal extractor（51）；The head tank（11）, pentanediamine convert integrated tank
（21）It is connected by circulation by pipeline with phytic acid calcium column, the PH adjusts flow container（31）It is connected to head tank by pipeline（11）With penta
Diamines converts integrated tank（21）Between pipeline on, the eluent holding vessel（41）Phytic acid calcium column is connected by pipeline, it is described
Centrifugal extractor（51）It connects on phytic acid calcium column.
2. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 1, it is characterised in that：Institute
State head tank（11）Integrated tank is converted with pentanediamine（21）Between having heaters is set（61）.
3. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 2, it is characterised in that：Institute
It further includes the first delivery pump to state feedstock transportation part（14）, the first solenoid valve（12）And second solenoid valve（13）；The head tank
（11）, the first delivery pump（14）, the first solenoid valve（12）And second solenoid valve（13）It is connected by circulation by pipeline, the heater
（61）It is connected to the first solenoid valve by pipeline（12）With second solenoid valve（13）Between pipeline on.
4. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 2, it is characterised in that：Institute
It further includes the second delivery pump to state PH and adjust part（34）, third solenoid valve（32）With the 4th solenoid valve（33）；The PH adjusts liquid
Tank（31）, the second delivery pump（34）, third solenoid valve（32）With the 4th solenoid valve（33）It is connected by circulation by pipeline, the heating
Device（61）It is connected to third solenoid valve by pipeline（32）With the 4th solenoid valve（33）Between pipeline on.
5. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 2, it is characterised in that：Institute
It further includes third delivery pump to state column absorb-elute part（44）, the 5th solenoid valve（42）With the 6th solenoid valve（43）；The elution
Liquid holding vessel（41）, third delivery pump（44）, the 5th solenoid valve（42）It is connected by circulation by pipeline with the 6th solenoid valve, the plant
Sour calcium column is connected to the 5th solenoid valve by pipeline（42）With the 6th solenoid valve（43）Between pipeline on.
6. a kind of device of immobilized cell continuous production extraction pentanediamine according to claims 1 or 2 or 3 or 4 or 5,
It is characterized in that：The phytic acid calcium column is divided into the first calcium column（71）With the second calcium column（72）, penetrate through the first calcium column（71）Setting the
One isocon（73）With the 4th isocon（74）, penetrate through the second calcium column（72）Second isocon is set（75）With third isocon
（76）；The first calcium column（71）With the second calcium column（72）The first isocon on the same end（73）With the second isocon（75）Altogether
With connection eluent holding vessel（41）, the first isocon on the other end（73）With the second isocon（75）Common connection centrifugation extraction
Take part；First calcium column（71）With the second calcium column（72）The 4th isocon on the same end（74）With third isocon（76）Jointly
It connects pentanediamine and converts integrated tank（21）, the 4th isocon on the other end（74）With third isocon（76）Common connection raw material
Tank（11）；First isocon（73）, the 4th isocon（74）, the second isocon（75）With third isocon（76）Upper difference
It is provided with the valve for controlling switch.
7. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 6, it is characterised in that：Institute
State pentanediamine conversion integrated tank（21）On be additionally provided with PH detection feedback systems（22）, temperature detection reponse system（23）And chuck
（24）, lysine concentration detection device（26）.
8. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 1, it is characterised in that：Institute
It further includes the extractant holding vessel being connected by circulation by pipeline to state Centrifugical extraction part（52）, the 4th delivery pump（55）, the 7th electricity
Magnet valve（53）With the 8th solenoid valve（54）, the centrifugal extractor（51）It is connected to the 7th solenoid valve by pipeline（53）With the 8th
Solenoid valve（54）Between pipeline on.
9. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 8, it is characterised in that：Institute
It further includes raffinate collecting tank to state Centrifugical extraction part（56）With extract liquor collecting tank（57）.
10. a kind of device of immobilized cell continuous production extraction pentanediamine according to claim 1, it is characterised in that：
The Centrifugical extraction part is at least provided with three centrifugal extractors（51）.
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| CN106984061A (en) * | 2017-05-17 | 2017-07-28 | 南京工业大学 | A kind of 1,5 pentanediamine continuous countercurrent extraction Processes and apparatus |
| EP3852923A1 (en) * | 2018-09-18 | 2021-07-28 | INVISTA Textiles (U.K.) Limited | Systems and methods for recovering amines and their derivates from aqueous mixtures |
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| CN105368766A (en) * | 2015-05-20 | 2016-03-02 | 天津科技大学 | Genetically engineered bacterium for producing pentamethylene diamine and method for preparing pentamethylene diamine |
| CN105777555A (en) * | 2014-12-17 | 2016-07-20 | 上海凯赛生物技术研发中心有限公司 | Continuous purifying device of 1,5-pentanediamine, and method thereof |
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| US8906653B2 (en) * | 2008-01-23 | 2014-12-09 | Basf Se | Method for fermentatively producing 1,5-diaminopentane |
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| CN103725724A (en) * | 2013-12-28 | 2014-04-16 | 天津北洋百川生物技术有限公司 | Method of preparing cadaverine by virtue of immobilized hafnia alvei |
| CN105777555A (en) * | 2014-12-17 | 2016-07-20 | 上海凯赛生物技术研发中心有限公司 | Continuous purifying device of 1,5-pentanediamine, and method thereof |
| CN105368766A (en) * | 2015-05-20 | 2016-03-02 | 天津科技大学 | Genetically engineered bacterium for producing pentamethylene diamine and method for preparing pentamethylene diamine |
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